LOCUS       DASJRQ010000001        80137 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJRQ010000001 DASJRQ010000000
VERSION     DASJRQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.001"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(212..952)
                     /locus_tag="U4A80_000001"
     CDS             complement(212..952)
                     /locus_tag="U4A80_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973676.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1082..2029)
                     /locus_tag="U4A80_000002"
     CDS             complement(1082..2029)
                     /locus_tag="U4A80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1973677.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2158..2922)
                     /locus_tag="U4A80_000003"
     CDS             complement(2158..2922)
                     /locus_tag="U4A80_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1973678.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2926..3315)
                     /locus_tag="U4A80_000004"
     CDS             complement(2926..3315)
                     /locus_tag="U4A80_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973679.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3450..3992
                     /locus_tag="U4A80_000005"
     CDS             3450..3992
                     /locus_tag="U4A80_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1973680.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4140..4772
                     /locus_tag="U4A80_000006"
     CDS             4140..4772
                     /locus_tag="U4A80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973681.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4906..5031)
                     /locus_tag="U4A80_000007"
     CDS             complement(4906..5031)
                     /locus_tag="U4A80_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1973682.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5165..6058)
                     /locus_tag="U4A80_000008"
     CDS             complement(5165..6058)
                     /locus_tag="U4A80_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1973683.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6240..7166)
                     /locus_tag="U4A80_000009"
     CDS             complement(6240..7166)
                     /locus_tag="U4A80_000009"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1973684.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7251..8012)
                     /gene="pflA"
                     /locus_tag="U4A80_000010"
     CDS             complement(7251..8012)
                     /gene="pflA"
                     /locus_tag="U4A80_000010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1973685.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8256..10487)
                     /gene="pflB"
                     /locus_tag="U4A80_000011"
     CDS             complement(8256..10487)
                     /gene="pflB"
                     /locus_tag="U4A80_000011"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1973686.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10764..13214)
                     /gene="parC"
                     /locus_tag="U4A80_000012"
     CDS             complement(10764..13214)
                     /gene="parC"
                     /locus_tag="U4A80_000012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1973687.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13226..15271)
                     /gene="parE"
                     /locus_tag="U4A80_000013"
     CDS             complement(13226..15271)
                     /gene="parE"
                     /locus_tag="U4A80_000013"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1973688.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15437..15871)
                     /locus_tag="U4A80_000014"
     CDS             complement(15437..15871)
                     /locus_tag="U4A80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1973689.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15998..16636
                     /gene="plsY"
                     /locus_tag="U4A80_000015"
     CDS             15998..16636
                     /gene="plsY"
                     /locus_tag="U4A80_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1973690.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16768..17643)
                     /locus_tag="U4A80_000016"
     CDS             complement(16768..17643)
                     /locus_tag="U4A80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1973691.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17725..18516)
                     /gene="codY"
                     /locus_tag="U4A80_000017"
     CDS             complement(17725..18516)
                     /gene="codY"
                     /locus_tag="U4A80_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1973692.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18534..19934)
                     /gene="hslU"
                     /locus_tag="U4A80_000018"
     CDS             complement(18534..19934)
                     /gene="hslU"
                     /locus_tag="U4A80_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1973693.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19948..20496)
                     /gene="hslV"
                     /locus_tag="U4A80_000019"
     CDS             complement(19948..20496)
                     /gene="hslV"
                     /locus_tag="U4A80_000019"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1973694.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20533..21438)
                     /gene="xerC"
                     /locus_tag="U4A80_000020"
     CDS             complement(20533..21438)
                     /gene="xerC"
                     /locus_tag="U4A80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1973695.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21636..23012)
                     /gene="trmFO"
                     /locus_tag="U4A80_000021"
     CDS             complement(21636..23012)
                     /gene="trmFO"
                     /locus_tag="U4A80_000021"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1973696.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22981..25059)
                     /gene="topA"
                     /locus_tag="U4A80_000022"
     CDS             complement(22981..25059)
                     /gene="topA"
                     /locus_tag="U4A80_000022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1973697.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25181..26038)
                     /gene="dprA"
                     /locus_tag="U4A80_000023"
     CDS             complement(25181..26038)
                     /gene="dprA"
                     /locus_tag="U4A80_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1973698.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26094..26861)
                     /locus_tag="U4A80_000024"
     CDS             complement(26094..26861)
                     /locus_tag="U4A80_000024"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1973699.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26854..27714)
                     /gene="ylqF"
                     /locus_tag="U4A80_000025"
     CDS             complement(26854..27714)
                     /gene="ylqF"
                     /locus_tag="U4A80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1973700.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27974..28372
                     /locus_tag="U4A80_000026"
     CDS             27974..28372
                     /locus_tag="U4A80_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1973701.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28651..29151
                     /locus_tag="U4A80_000027"
     CDS             28651..29151
                     /locus_tag="U4A80_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1973702.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(29234..29785)
                     /gene="lepB"
                     /locus_tag="U4A80_000028"
     CDS             complement(29234..29785)
                     /gene="lepB"
                     /locus_tag="U4A80_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1973703.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29937..30164)
                     /locus_tag="U4A80_000029"
     CDS             complement(29937..30164)
                     /locus_tag="U4A80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1973704.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30161..30679)
                     /gene="msrA"
                     /locus_tag="U4A80_000030"
     CDS             complement(30161..30679)
                     /gene="msrA"
                     /locus_tag="U4A80_000030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1973705.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30699..31412)
                     /locus_tag="U4A80_000031"
     CDS             complement(30699..31412)
                     /locus_tag="U4A80_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1973706.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31415..32311)
                     /locus_tag="U4A80_000032"
     CDS             complement(31415..32311)
                     /locus_tag="U4A80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1973707.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32469..33311)
                     /locus_tag="U4A80_000033"
     CDS             complement(32469..33311)
                     /locus_tag="U4A80_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1973708.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33443..34096
                     /locus_tag="U4A80_000034"
     CDS             33443..34096
                     /locus_tag="U4A80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1973709.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34159..34686)
                     /locus_tag="U4A80_000035"
     CDS             complement(34159..34686)
                     /locus_tag="U4A80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1973710.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34706..35653)
                     /locus_tag="U4A80_000036"
     CDS             complement(34706..35653)
                     /locus_tag="U4A80_000036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1973711.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35665..37551)
                     /locus_tag="U4A80_000037"
     CDS             complement(35665..37551)
                     /locus_tag="U4A80_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1973712.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37711..39789)
                     /locus_tag="U4A80_000038"
     CDS             complement(37711..39789)
                     /locus_tag="U4A80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1973713.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39802..40077)
                     /locus_tag="U4A80_000039"
     CDS             complement(39802..40077)
                     /locus_tag="U4A80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973714.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40439..40894)
                     /locus_tag="U4A80_000040"
     CDS             complement(40439..40894)
                     /locus_tag="U4A80_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973715.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41048..42256)
                     /locus_tag="U4A80_000041"
     CDS             complement(41048..42256)
                     /locus_tag="U4A80_000041"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1973716.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42368..42700)
                     /locus_tag="U4A80_000042"
     CDS             complement(42368..42700)
                     /locus_tag="U4A80_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1973717.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42848..43723
                     /locus_tag="U4A80_000043"
     CDS             42848..43723
                     /locus_tag="U4A80_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1973718.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43805..44386)
                     /locus_tag="U4A80_000044"
     CDS             complement(43805..44386)
                     /locus_tag="U4A80_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1973719.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44392..45651)
                     /locus_tag="U4A80_000045"
     CDS             complement(44392..45651)
                     /locus_tag="U4A80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1973720.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45653..46699)
                     /locus_tag="U4A80_000046"
     CDS             complement(45653..46699)
                     /locus_tag="U4A80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973721.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46934..47209)
                     /locus_tag="U4A80_000047"
     CDS             complement(46934..47209)
                     /locus_tag="U4A80_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1973722.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47452..48762)
                     /gene="der"
                     /locus_tag="U4A80_000048"
     CDS             complement(47452..48762)
                     /gene="der"
                     /locus_tag="U4A80_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1973723.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48944..50173)
                     /gene="rpsA"
                     /locus_tag="U4A80_000049"
     CDS             complement(48944..50173)
                     /gene="rpsA"
                     /locus_tag="U4A80_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1973724.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50308..50988)
                     /locus_tag="U4A80_000050"
     CDS             complement(50308..50988)
                     /locus_tag="U4A80_000050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1973725.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51037..51672)
                     /locus_tag="U4A80_000051"
     CDS             complement(51037..51672)
                     /locus_tag="U4A80_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1973726.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51738..53141)
                     /locus_tag="U4A80_000052"
     CDS             complement(51738..53141)
                     /locus_tag="U4A80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1973727.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53128..54159)
                     /locus_tag="U4A80_000053"
     CDS             complement(53128..54159)
                     /locus_tag="U4A80_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1973728.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54190..54411
                     /locus_tag="U4A80_000054"
     CDS             54190..54411
                     /locus_tag="U4A80_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1973729.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54576..55313)
                     /locus_tag="U4A80_000055"
     CDS             complement(54576..55313)
                     /locus_tag="U4A80_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1973730.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55466..56806)
                     /locus_tag="U4A80_000056"
     CDS             complement(55466..56806)
                     /locus_tag="U4A80_000056"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1973731.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57043..58566)
                     /locus_tag="U4A80_000057"
     CDS             complement(57043..58566)
                     /locus_tag="U4A80_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1973732.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58944..59555)
                     /locus_tag="U4A80_000058"
     CDS             complement(58944..59555)
                     /locus_tag="U4A80_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1973733.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59678..59803)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59885..60601)
                     /locus_tag="U4A80_000059"
     CDS             complement(59885..60601)
                     /locus_tag="U4A80_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1973734.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60607..61176)
                     /gene="scpB"
                     /locus_tag="U4A80_000060"
     CDS             complement(60607..61176)
                     /gene="scpB"
                     /locus_tag="U4A80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1973735.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61160..61957)
                     /locus_tag="U4A80_000061"
     CDS             complement(61160..61957)
                     /locus_tag="U4A80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1973736.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61927..62298)
                     /locus_tag="U4A80_000062"
     CDS             complement(61927..62298)
                     /locus_tag="U4A80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1973737.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62317..63204)
                     /gene="xerD"
                     /locus_tag="U4A80_000063"
     CDS             complement(62317..63204)
                     /gene="xerD"
                     /locus_tag="U4A80_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1973738.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63300..63773)
                     /locus_tag="U4A80_000064"
     CDS             complement(63300..63773)
                     /locus_tag="U4A80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1973739.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63878..64741)
                     /locus_tag="U4A80_000065"
     CDS             complement(63878..64741)
                     /locus_tag="U4A80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1973740.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64937..67036)
                     /locus_tag="U4A80_000066"
     CDS             complement(64937..67036)
                     /locus_tag="U4A80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1973741.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67256..68365)
                     /gene="rpoD"
                     /locus_tag="U4A80_000067"
     CDS             complement(67256..68365)
                     /gene="rpoD"
                     /locus_tag="U4A80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1973742.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68389..70230)
                     /locus_tag="U4A80_000068"
     CDS             complement(68389..70230)
                     /locus_tag="U4A80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1973743.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70412..72727)
                     /locus_tag="U4A80_000069"
     CDS             complement(70412..72727)
                     /locus_tag="U4A80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1973744.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72912..73772)
                     /locus_tag="U4A80_000070"
     CDS             complement(72912..73772)
                     /locus_tag="U4A80_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1973745.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73831..74352)
                     /locus_tag="U4A80_000071"
     CDS             complement(73831..74352)
                     /locus_tag="U4A80_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1973746.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            74630..75391
                     /locus_tag="U4A80_000072"
     CDS             74630..75391
                     /locus_tag="U4A80_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1973747.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75417..78008
                     /gene="mprF"
                     /locus_tag="U4A80_000073"
     CDS             75417..78008
                     /gene="mprF"
                     /locus_tag="U4A80_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1973748.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78196..79383)
                     /gene="tuf"
                     /locus_tag="U4A80_000074"
     CDS             complement(78196..79383)
                     /gene="tuf"
                     /locus_tag="U4A80_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1973749.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79751..80107
                     /locus_tag="U4A80_000075"
     CDS             79751..80107
                     /locus_tag="U4A80_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1973750.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        21977 a        16844 c        13742 g        27574 t
ORIGIN      
        1 tttataaaaa aataaactat ttttgcaaac gatctgcgaa aatagtttgt ttttttattt
       61 tgccattatt ttcttactta ttgggagaaa aaattcgctt aaactcaaaa aaatttgagg
      121 atacgatata aactttcgga tagagtataa aataaggaaa cgccgcagcg tttcctttcc
      181 aactgtagcc gaatataaat ttgtatatca cttatttaaa atagctattt tctcccaaag
      241 agtttccttg atcatcgatc aatccttgta atgagcgttc taagatacca tggctttttt
      301 tcgatttttt ttcttctgac tgctttgttt cctctaacgt ttttgttcga ttgaatgttc
      361 gtacagacgg ctcaccattt cttttttctt gatacggcgt cggttctgta tcgaacaaca
      421 gataagagtc atccgttttt ttcatcgatt cgattaaatg atcctcagaa attttgcttt
      481 ctctagggtc tgggataatc gatgcgggta catatttagg aacaaaataa gaacgctccc
      541 gcaccttcgt gtcaacagat gcgtttcgtg tagtatgctt ggactctgtc cttgtttcac
      601 tttgtctgac agaacgagag gaatcaccaa aaagtttttt tcgtcctgtc gatgtgattt
      661 ctgattggta atggccggta taatccggta attttccttt atgcttttcc aaattttcct
      721 tacgcggagc tgacttcaat ccatttgttg cacgacgctt gcgacggatt ttctcggtgt
      781 tcgtgttcgt gaaatgaaat tctttcatct gttcaaacgg ggactgattc tgttcagtca
      841 aaagatcttc ttgtccgtca aataatactc gcttcttgtc gttggttaat tttacgccta
      901 actcatcatc gtacgcagga aaacggaaat gttttcgtct gatttctttc attcaaacca
      961 tccttctcaa aaatggaact tttctcaata atagcacaga ttcattgttt tcagtagcaa
     1021 tgcgctgatt attttaagaa aatagcgatg aatttcataa atatatatat agtaataaga
     1081 cttaattcgt tgtctctctg ataactagtt caggaaaaaa acgacgataa ccttgactcg
     1141 cccctttttc ttctattttt tttaacaaca tttctccaca tacttcaccc atacgtataa
     1201 tatcttgctt gatcgttgtc aatttaggtg aactgatttg gtctaaaaat accccatcaa
     1261 atccaatcac accaaattct tcgggcaagt gtcctccagc atttataatc cctcttactg
     1321 ctcctagtgc tagccgatct gaactacata caaaacaagt tttatctgta aaatctgccc
     1381 agttttctgt catatatctt tctacttctg tcgaatgatt tggaaaccgc aagattttac
     1441 taggaagatc aacttccttc atcacagata aataaccagt ttcccttgaa atttcaaaag
     1501 gctcgtccac ttcgataccg atataaataa tcgtttgata atctctttct aaggcatatt
     1561 gcgttgcttt tgctgtacca tattcattat cgctgtctac ataatcatat ccttcagaat
     1621 tttccccaaa caagacaact ggtttttcca aacggtcgat ccaatcgaag tctttttttc
     1681 tgacacctgt gatgatgtat ccatcacaag aaccgacgtc gaaggcatca tttgtaacta
     1741 attggagaga ataatgcgct ttaccgatcc gtttagcgat tcccatcagc aaattcatgt
     1801 aatagggctc agttgtgtcg atttcttcca gtataaataa tttgatgatc tgcgaccgat
     1861 tcgtgacaag tgcttttgca gcaatatttg gacggtaatc cagttcagcc attgctgaga
     1921 aaactaactc tttcaattcg tcagtcacct gttccggatg attgatgact cgagagaccg
     1981 tcatttttga aacattggca cgttttgcaa catctgctaa agtgggcatg ctctcttcct
     2041 ttctaacatg ttgtcattct tactatcaat tataccaaat tttcctaaca gagtttatta
     2101 tagcccaaaa taaaaaacgg aaaatcctct atcgtcatga gtgattttcc gtttcaatta
     2161 tttatacttt tttatcaaat tttttgctaa aaaagtggaa gcttcttcca ttagtggtat
     2221 ttgtaccata tgtcgctgtg tttttgagga acgaaaaaca atttcttttc cgtaagactt
     2281 atgccgattt ttttctacaa atcgttccgt ttgtatataa gggatttttt cgtcagcttg
     2341 tccatgccaa aagaacagag gacgtccagc taacttttcc ggctgcaatg acaaatcata
     2401 gctcttaacc catgaaatca aatcaaaata gtcttttggt acgacataat tatattccga
     2461 tgcgcgcctt acgatttctt ttccatattc caaaggtgca ggggaaccca tcaaacacac
     2521 gcccgctact atatgaggat gtttagccaa caaggcgcat gttgtcattc cccccatcga
     2581 aaccccgcca actgcaagct gatcatgtag aagacctctt ttttctaaat gcttgataag
     2641 tgtgtcaaat tcaaacagat ttccgtagat actatcccaa aatgtatagg atggaataga
     2701 agaaaccggc tgtttgcgct ccccatgatt catcgcatct ggcaggatca cacgaatgcc
     2761 ctgctgcgca atttttcttc cttgcgtcaa aactaactcc ttacttgtct gccatccgtg
     2821 ataatagatg acaacgggaa tacgttcctc tcttaattct ttcagaacaa cttctaacac
     2881 tggaatatga tggacagaac gtttcaatat aattggattc aattgttact cacctatttt
     2941 cattaaattt cctgatcgcc atagcaacca tgctccaaca gttgtctgga acaggttcag
     3001 tgtaccaaat aatataaatg caggactgaa tggctcggga aataagcaca gagaacaaag
     3061 tcccaccact cctaatgcta gcgaaaggag tggataaata gaaaaattca aaaaactggc
     3121 aacgctaccg aaaatattac caataaacag tatcaaaaag aaaaaaacgc ttaaaattaa
     3181 accatctgtc agcgacatct gaaaaacaat gttctctacc tcaattggct gttgaatcga
     3241 atagacacct aagtatatac tgagaaggag acttagcacg tggctggcac aaaacacacg
     3301 attgaccgtt ttcattttaa tcacctaacc tatctttata attctattat aatcatttta
     3361 tcattttttt gtatgtataa agtttcaatt tttcttcact tacgattttt tttgatattg
     3421 ttgttatata aatgaaagga gttttacaaa tgattgatcc aaaaatgttg gggatgatct
     3481 tcctcattaa ttttgcttat atcactctga acacgttacg tttcatgttg acaatgaaag
     3541 gctatcgact gattgctcca cttgtcagta tggtcgagat cacgatttat atcgtcggct
     3601 tatcccttgt tttagatcgt ttagataatc cactgaattt atttgtctat gcgttaggtt
     3661 acgcagtagg tatcagtgtc ggaattttga ttgaagacaa attggcatta ggatacatca
     3721 tggtcacaac gattttgcca gcaaatagcg cagaagataa aagtttgccg ggtatactga
     3781 gagaacacgg ttatggtgtg acacagactt ctgcaatggg gctggaaggt gagcggctag
     3841 ttctcgaaat actttctcct agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa
     3901 tcgaagagcg tgcttttatc atttcttatg agccaaaata tatttcaggt ggtttctgga
     3961 ctaaaaaagt gagaaaacga caaggaaaat aactcacaaa cttaaaaact gtctaatatc
     4021 taaattaaat tttaaatgtt agacagtttt tatttattat tttgattaaa aaacatcgac
     4081 atatactcat caatttgtta ttatacttta tatacttatc aatttaattt ggagatatta
     4141 tggaaattca acgtagaacc actaattttt tacatttagg ctcttttttt gcaactttga
     4201 cgatcttatt ttttctatgt tattcatacc ttaagacctc acatgcgcct tctagtgcat
     4261 taggctggtt gcttttggat cattctgata tcacagaagc cgaagaaaat ccgttttttt
     4321 tgattttgtc ttctctttgc ggcttactgt tttctgttta tttcggtgcc tatttttttc
     4381 atcgacatcc tggtacgtta aacgaagctt caagcgtatt gattgcattt aaaacgtttt
     4441 cttttttagc aattcttttt tattcgttta atcacaatac gcttttcttc tttatcatga
     4501 atggtttagt cgtgatttta tccattttta cttattttat tttttcactc atttttagcc
     4561 aattgaaatg tccgctcttc tttcgattag ttcctgtcac ctgttttatt tacagcttgt
     4621 tttccttttt tgtccttgct ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag
     4681 gaaatctgtt ggatatgctc ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa
     4741 acaaacaaaa taaaaagcat tttgaagcct gactattacg acttcataaa tgctttttta
     4801 gttttaaaca cttcaatcta aaagaataac ccaagattgg ataaattatt aatgactcgc
     4861 cattcgtttc ttagtgattc tggattatca aatggacttc ctaatctaat cagctagttc
     4921 ttcccataaa atgccatact tgactgaatc ataatattga ttatttaaaa atctaacttt
     4981 tcttatcact acttcctttg tcattccaca tttctcacca tttttttgca ttcctttgtt
     5041 tccagaccat gtagtgaatc taataggtgg tagataggca aaatactcaa ataattcttt
     5101 taaccataaa ggaagagaaa ggctcctcct gcaagagtca gtataatttt tgcttactgc
     5161 tctcttaggt tttactcact aaaactgttc catattcttt gatataagaa aatgcatgcg
     5221 gtataaattt cttgctcagc tcagaaaaga tttgtcgttt aagaaaggct atttcctcca
     5281 ttgtcaatga aggatccaat gttggtaatt caatctcaaa attgtttttt tcgacaatag
     5341 gtaattctaa tgtgaatgat tcttcttcct ttatagtatt cgtcacaagt agagactgat
     5401 ggaaaatact aggccggcga tttttatatt tattcttacg tatcctgtca acgataactt
     5461 ctatttgatc aaaaaagtac gattcattta cgtctccaat aagttcggct aattctttga
     5521 atgccttcgt tacaggaagc tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg
     5581 ggaagtgttc tatagaggaa agtgtgataa aacggtactt ctcatttcct gcagttgtcc
     5641 tcagcaattc tgtctctcgg ctgtttctta ctccataaaa atacgcttct ttccgttcat
     5701 cttcgaaaca atacgcccaa ataacttctc ccatcgctaa tttttgttca tcaacagaga
     5761 gtctcttcaa ttgttcagct atggtttcag cagccgaagt ggcttgttgc aagtaatctt
     5821 taggatcaaa tattggaaag ttcaaatgat atcttcgttc ttccctcttg attaatcctg
     5881 catcgatcaa taaatcaaga aaatgggaaa atttattttg cgaaaattct tttttcaatt
     5941 ctcttagaat cgtttcttgt tctcgatgtt catatagata atcggtcaat gaagaaaaaa
     6001 gcggatcatc ggtcagtttt tgataaaagt tttccttatt ttttgaataa tagttcatat
     6061 atgttctctt ttctatccgt tttattttac ttaaaaggct tttacgtaaa aaggaggaag
     6121 aacaagagtc ataattgact gttattctcc tccatttctt cggcctttag tatgttctat
     6181 gtttcttttt atatcaatcg cttatccatt cattttgaat gcttcatact accatttgat
     6241 tagctaaata cagccgtcaa ttgaggtacg acttgttttt tacgtgaaac gacaccttta
     6301 agcaaagcac ggttgttggt caaagttgtt tggaaagctt cttctacttt ttcttttcct
     6361 ttaccagcaa caagtaattc tgaatcactg tctaagatgt ttgtgacaac taatacgaac
     6421 agatcgtagc catttgcttt catttcatct tccattgctg cttctagctc tgcttgtcga
     6481 tccatcactt cattaaggtc cactgtattg acttgagcga tacgaacgtt ggaactgccc
     6541 attgggaaac tttttgcatc caaatcaaga agaaccgcag ctgatttatc acttagattt
     6601 gttcctgctt tcaacatttc aagtccataa gtgtttagat cgacttcagc gattgctgca
     6661 agttcttttg cagctgcttc atcttcaggc gtacatgttg gtgatttgaa caacaatgta
     6721 tctgaaatga tagctgagag catgattcct gcgatttctt taggaattgt tacttggttt
     6781 tctttatata gttttaaaat aatcgtactt gtacatccaa ctggttctgc acgatagtac
     6841 aatggattag ctgtttcaaa gtttgcaata cgatggtggt ctactacagc taaaatattt
     6901 acttcgctga tatctgaaat actttgttgg aattcattat gatccacaag catgacatct
     6961 gcattttctg ctttagctga ctctactact cgtggtgcag atagctgaaa gtggtctaat
     7021 gcgtattgtg tttcttcatt aggtgtacct agagcaactg cttctgtttc tttacctaat
     7081 tcattttgca agtgtgaaaa cgcaatggct gccccgattg catctgtatc tggattttgg
     7141 tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt tgtgatttct cctatttaat
     7201 aatagcaaaa aaacggaaag aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt
     7261 aaatattttg tatactcttc tgtatgaagc aatcgtttag cattttcgac acgttctttt
     7321 gtaggtggtt cgatcccttc taatggatat ttcaaaccaa gtgtttccca cttgtatttt
     7381 cccatcgtat gataaggaag aatctctact ctgtctacat ttgacaaggt ttgaataaag
     7441 ttgtctagtc ggatcaaata ttcatcataa tcacttcgct caggaactag tacatggcga
     7501 atccaaaccg gtttgttgat ttttgacaaa tattgagcca tttctaaaat attttcattg
     7561 tcccaatgcg ttagtttttt atgctcttca ttatcgatgt gtttgatgtc aaataacagt
     7621 aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa
     7681 ggttttccgc atgtatccaa tgtagtatgc atcttccgct cttttgcttt tttaaatagt
     7741 tcgatcaaaa aatcgatctg caatagaggt tctccaccac taacagtaat accaccttta
     7801 tctccccagt aaggtctgaa gcgttctgct ttatctaaaa gttcatctgc tgtgtattct
     7861 ttccctccgc caatattcca agtatctgga ttgtgacaaa attggcaacg catgcggcat
     7921 ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata
     7981 gaatgaacat agccgattgt tttttcttcc atttaagttc acatcttttc tttgatttca
     8041 ttttaagtac aagttagctg tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc
     8101 gcttcgttat gtgtaaaatt tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt
     8161 ttctttcaaa taagcatact gattattcga ttttgatgcg atggctattc aacgtccttt
     8221 cgacgctaag tcgaaaaact tttttcacga actttttaca ttctgtcgtg tgaagttctc
     8281 atcaagacat ctaattgttg ttctcttgtc aagtcacgga atttaactgc ataaccagat
     8341 acccggatcg tcaagttcgg atatttcatt ggattttcca ttgcatcacg caataaatca
     8401 ttactgaata cattgacatt caaatgataa gctccttttg agaagtatcc atctaagaca
     8461 ttacgcaagt tttcgattct tgtatcttgt tcttttccaa gacctgaagg attgattgtt
     8521 tgggtatttg aaattccatc caatgcacag ccgtaattca ttttagctgt tgagttcagg
     8581 ctagctaata acccactttg ttccgcattg taacttggat ttgctcctgg cgcaagtggt
     8641 tttccagctt cacggccatc aggagtattt cctgtatttt taccgtaaac aacatttgaa
     8701 gtaatcgtca ataatgaagt tgttggtttt gagttgcggt aagttttatg acgtttgatt
     8761 tgtcccatga agtattccaa gatccatgaa actaattcat ctgcacggtc atcatcattt
     8821 ccgtatttag ggaagtcgcc atccacttca aaatcaactg ctattccttt ttcattacgg
     8881 attgctttta cttttgcata tttgatcgct gacaagctgt ctgccgcatg agagatccca
     8941 gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca tcaaagccag ctgagctgct
     9001 tcataagaat atttatcatg catataatga atgatgttca acgtattgac atatagttct
     9061 gctaaccaat ccatcatatt catgaagtta tgtttcactt cttcaaagtc tagtatttct
     9121 gatgtgattg gacgatattc tggtccaact tgggcgccag tcacttcatc gactccgcca
     9181 ttgatggcat ataatagtgt tttagccaag ttagcacgag cgccaaagaa ttgcatgtct
     9241 tttccgacaa ctgttcctga tacacagcaa gcaatcgcac aatcatcaga accccattgt
     9301 tcacgaagct gattatcatt ttcgaattga atcgaagaac tttcgatagc gattctgcta
     9361 gcatatgttc tgaatccttc tggaaggtgt tcagaataca aaacagtcaa gtttggttct
     9421 ggtgaaggtc ccatatttgt taatgtatga agcagacgga aatcattctt tgtaactaat
     9481 gaacgaccat ctaatcccat acctgcaatg gataaagtag cccagatagg gaatcctgag
     9541 aataattggt tatattcagg tgtacgcgcg aatttgacca tacgtaattt cattactaag
     9601 tggtcgatca attcttgtgc ttctttttct gtgattactc cattatccaa atcacgttgg
     9661 atatagatat caaggaatgc tgagatacgt ccgattgaca tcgcagcacc attttgtgat
     9721 ttgattgctg ctaaatatcc gaagtatagc cattggacag cttcttttgc atttgcagct
     9781 ggtttagaaa tatcatagcc atatttcgca gccatttctt tcatttgcgc taatgcacga
     9841 tattgttcag tgatttcttc acgtaaacga atcacttctt cactcattgt atgataacca
     9901 gtcatatcat gatctttttt cttttgttcc atcaaatagt cgattccata caatgccaca
     9961 cgacgatagt caccgatgat acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt
    10021 ttgtttttac gagcagcacg catttcagca gtataagcgt caaatacacc ttgattatgt
    10081 gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt ttggtgtata gccattgctt
    10141 tttagcgcat tgttagccat attgattccg ccgaacggca tgaatgcttg tttcaaaggt
    10201 ttgtctgtct gtagaccgac gattttttcc aaatctttat ccaaatagcc tgcatcgtgt
    10261 gaagtaatag ttgatacgat ttctgtgtcc atatcataga caccgttacg ctcgaattgg
    10321 atatcattta acttttgcaa ttcatcccat aatttttctg ttgcttctgt tgggccttcc
    10381 aaaaagctgt catcaccgcg atattcagtg tagttatttt gaataaagtc tctagtgtct
    10441 acgccttctt tccatttggt acctttaaat ccattccatt gctccattgt atggacctcc
    10501 taaatttctg ttttatgtaa cagtttttac gtgattagta taacacagca ccctatcttt
    10561 gcaagggttt tcttgttttt tatttaacaa tttttatgtt ttatttacat atatttaaat
    10621 caacgttgac ataccaacat ttaagcattg tgaacacttt catctttttt tgcaaacgta
    10681 taaggattta ttaaaaatga atgaaaaaga gacgccacct gtattaatac agatggcgtc
    10741 tcttcattaa aatactacaa cagttacgac tcgatgaaaa tctcaggagc atcgataact
    10801 tcgatgactt ctccaccttt tttctcatcc atcgcaaatg atccattcga tgttcgatca
    10861 cctataggga aatcagttgg gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt
    10921 atttcttttg atcttccttc cgacatatag ataatacggt gcggattttt tttcagctca
    10981 cgcatgaccg tcactccccg cttcgcacgt gaagtttggc tgatttcttg agcaagcatg
    11041 cgtttaacag ctcctcgctg agtaacgatt acgatcggcg tatcaccttc tgctaacaca
    11101 agcagcccgt tgataatcct atctccatct ttcaaattca tagacttcac tccagctgcc
    11161 tttgctccaa caacgggaac atctgttaaa ggatagcgca aacccattcc ttgatgcgtt
    11221 actaagaaaa catctaagcc tggctgatct tctttcagat acacagcaat cagttcatct
    11281 tcttccgcct ttagcttcat acagctgagc ggacggctct tataggtgcg ccatggttcg
    11341 aactctgtca ttcgtgtttg cttgatcaat ccattcttgc tgatgaatac aaatgttttc
    11401 tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg attcgtctac agacagattg
    11461 gagattgttt gagaaatgtg ctcaccagca tctttccatt tcaaatcggg tagttcatgg
    11521 acagggcggt agatcacatt tgctttattt gtgatcagaa gaacatggtc caatgtactc
    11581 aattcacctg agtatagcaa gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa
    11641 gcactataag aacgaaggct tgttcgcttc acatagcctt ctcgcgtcac actgacaatt
    11701 acgtcttctt gtgccactaa gacttgcgta tcaattttga tttcttcgat ttcgtcttcg
    11761 attgtcgtta gacgagcgga agaataattt tttttcactt ctcgcaattc ttttttcata
    11821 acagagaaaa gctctttgtc atttgaaagg attttattca actctgtgat ttgcgcaatc
    11881 aagctttcgg attccttttg aagctctgtg atatccgtgt tcgtcaaacg gtataattgc
    11941 aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa agacgtcgac taaattcttt
    12001 ttggcgtctt ttttgtcttt actttcacga atcgttgcga ttacttcatc cagaatcgat
    12061 aaagctttca tcaatccttc tacgatatgc tgtctttttt gagctttggc taaatcgaat
    12121 tgactacgtt ttgttatgac ttgctttcga tgctcgatat aacttcttaa aatgtctttc
    12181 agtccaactt gatgcggagt catatggtcg atagcgacca tattgaaatt gtaattgatc
    12241 tgtagctcag tgtttttgaa caaataattc aaaattcctt gagcattggc gtcttttttc
    12301 aattcgacga caatttgcag acctgttcta tcactttcat ctcgtacttc agctattcca
    12361 tcgattttct tattcaaacg gatctcatcc atttttttca ccaaggtcgc tttatttact
    12421 tcatatggga tttctgtgat cacgatttgc tgtttattac ccttcattgc ctctatcttt
    12481 gttttcgaac gaagaataac ttttcctcgt cctgtttcat aagctttttt gatttcatct
    12541 tttccttgca agatccctcc tgttggaaag tcaggtccag gaatatattc catcaatttt
    12601 tcaaggctcg cttgtggatg atcgatcata tagattgtgc catcgatcac ttcagctaag
    12661 ttatgagtcg ggatttctgt tgcatatcca gcagatatac ctgtcgatcc attgacgaga
    12721 aggtttgggt atcgagctgg aaggactgtt ggttcttttt ctgtatcatc aaaattccac
    12781 acgagatcga ctgtttcttt ctcgatatct gctagcatct ctccacttaa cttggataat
    12841 ctggcttcag tataacgcat cgcagcaggt ggatcaccgt ccatactccc gttattcccg
    12901 tgcatttcga tcagtacttc ccgcaacttc cagtcttggc tcatgcggac catcgcttca
    12961 taaatactga tgtcaccatg aggatgataa ttccccatga tatttccgac agattttgct
    13021 gatttacgga atcctttgtc atacgtattg ccatctttgt tcatggcaaa taatatccgg
    13081 cgttgcactg gcttcaatcc atcacggata tcaggcagtg cacgttcttg gataatatat
    13141 ttggaataac gtccgaagcg gtcacccatc acttcttcta aagttaattc ttgtacttct
    13201 tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa taactgattg ttttcatttt
    13261 tatctgctcg ttcttcatct aataaatcat tagaaactga aggagagatt tcagtatcct
    13321 cttttttatc taagatactg ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc
    13381 attttcttcg aggttctact ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg
    13441 cagcatcatc gatacggacg cggatcaacg tacgggaagt cggatccatc gttgtttccc
    13501 acaattgttc cgcattcatc tctccaagtc ctttatatcg ctgcagcata tatcctttgc
    13561 cgactttttt aatcattgct gccaattcgt catctgtcca cgcatattca atgacttgct
    13621 tctttccttg tcctttggat actttatata aaggcggtaa cgcgatatat actttcccag
    13681 cttcaatcag cggtttcata taacgataaa agaaagtaag cagtaatact tgaatatggg
    13741 cgccgtctgt atccgcatcg gtcatgatga ttactttgtc gtaattacag tcttcgatag
    13801 aaaactcagg accgactcct gcaccaatcg tatagatcat cgtattgatt tcttcatttt
    13861 tcaagatatc ttgcatttta gctttttcgg tattgatgac ttttcctcgt aaaggtagaa
    13921 ttgcttggaa ctttctgtct cttccttgtt tagcagatcc gccagcagaa tccccttcga
    13981 ctaaatacaa ttcatttttc ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa
    14041 gaagagattc gcctttttta cgcttcttcc catttcggct ttcttctctg gctttacgtg
    14101 ctgcttctct cgcttctctg gctttgattg ctttacggac caacatctgg ctcatttcgc
    14161 tattttcctg caaataaaag cccatttgtt cactgatgac gttatctact acggtacgag
    14221 caactggcgt accaagtttt tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta
    14281 cacggataga aagtacagca gctaaccctt ctctgaaatc actaccttcc agattcttgt
    14341 cccgttcttt cagcaaacca acttttctcg catattcatt ataagatttc gtcatcgccg
    14401 ctttcatacc agcttcatgg gttcccccat cttttgtccg aacgttattg acaaaagata
    14461 aaacattttc tgaataaccg tcattgtatt gataagcgac ctctacttcg ataccttctt
    14521 tttctcctga aaaataaaca actggtgtca atgtatcttt ttcttcattc agataatcaa
    14581 caaattcttt gattccttct tcgtaatgaa aaatttcctt gactggttct tctccacgaa
    14641 gatcagaaag agagatttta acgcctttta gaagaaaggc agattctctt aaccgttcgg
    14701 ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt atcatctggt aaaaaatgga
    14761 cagtcgtccc gtttgctttt tttgtttttc ctatcttttt caaagtaccg tcaggtttac
    14821 cgccgttttt aaatttctgc tgatactcta cgccatctct aacgatcgtt acaattagcc
    14881 atttcgataa ggcatttacc acactggctc ctacaccgtg cagtccacca gaagttttgt
    14941 accctccttg accaaacttt ccgccagcat gcagcacagt aaaaatcact tcaactgttg
    15001 gtattccgga agcatgcatt cctacaggca tcccacgtcc gctatctgca acagttatac
    15061 tattgtcttc atggatggtc acatcaatct cactgccata acctgataat gcttcgtcaa
    15121 ctgcattatc gacgatttca tagactaaat gatgcaagcc tcggctgtca gtggatccaa
    15181 tgtacatccc tggtcttttt ctaactgcct ctaatccttc caatacttgg atggacgagt
    15241 cattgtattc gttgtttact ttttttgcca aggcaaacgc tccttcaata atactttgcg
    15301 gcaaaaccgc accacgatta tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga
    15361 gaaatcaatt ttaattatct cctaaccgct ttttcgacat tcccctctat cgatcatctc
    15421 ttctttgtgg tcatttttat ttttggtttt ctttcaactt accaagttca atcttaatac
    15481 aacgatccat tacgatatct gtacgtcctg cttttcttag catctcagct gcttctttgt
    15541 tttcaatccc taattgtgcc cagaacactt tagcatcagt tttcaaaaaa tcacctgcta
    15601 tttcaggtaa aaattcactc cgtctaaata catctacgat atcgattgga tcatcgattt
    15661 ctgtcaaatt cgcatagact ttctcgccta gaatttcttc accagccaat aatggattta
    15721 ctggtatgat tttatatcca ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt
    15781 ctggtttatt gctcaagcct acgacggcaa tattttttgc ttcttccaaa tagttgaaaa
    15841 tcgtttcttg tgttggattt tttacagaca tttctgccac cccttgcttt tctttttatt
    15901 ccatctatta tacttgtatc tttcattttt tcaattgttc ctgttaccta ttcatgctaa
    15961 aataaaggat agttttcaac ttgaaagagg gtaaaaaatg aaattgatct tattacttat
    16021 tttagcatat cttttaggtt ctattccttc cggtgtctgg attggcaaat tgtttttcaa
    16081 aaaagatatc cgacaacacg ggagcggtaa tactggaacg accaatacat ttcgtgtatt
    16141 aggcaaaaca gccgggattg ttgtcctgct tatggatatt ttaaaaggta cattggctac
    16201 ttgtttgccg atgatcttcc acctttctgg gattaaccct ctttggtttg gggtatgcgc
    16261 cattcttggc catacatttc cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac
    16321 aagtgcagga atgcttttag gatttaatcc tgtatttttt gtttattctt gtatcatctt
    16381 tatcgttagt ttattttgta caagtatggt gagtttgagc agtatgatca gtgcagtatt
    16441 gatcacttta tctacgatcg tccttcctta tgtagctcct gtgattttgg ctaagcccaa
    16501 ttggttactg acgatcattg cctttttagt ttcttccttt atcttttatc ggcataaaga
    16561 caatatcaag cgtatcagaa accatactga aagtcgaatc ccgtttggtt tgaatagcag
    16621 caagaaaaaa tcctgatact ttttcattcc aaagagataa aacgggagtg tgacaaatcg
    16681 atgtcacact cccttattac ttacaggata gcttttttac acaccttggt aaacggtgtt
    16741 ccctgctaac ttctcggtca caacctctta cttcactgtg atggaatatt ttgtcttgaa
    16801 gatttcatgg gcacctagtt gattgatccc tactttttcc atcaagtttc cactactgtt
    16861 caccgtgtca gcgattccgc accacggttc gatacaaacg aaaggcgctt cttttggata
    16921 aggagaccag atccctacat aaggcatatc tttatagctt aaagttacgc tatgggaaga
    16981 tttttcactt cgaatggcgt acgcatttag accccttgtt tcaaaaacca aggcatcttg
    17041 ctcaaaaagt tcacgattca aagccagatt cgtatttgtc tgacctaacg tcttttgatc
    17101 catatcgatg aatgggcctt tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc
    17161 cagatagaaa tcttcaaatg acaatccttc ttcaagaggt acattgaaag caggatgtcc
    17221 accgatagaa aagaacattt ctttttcacc ggtatttgcc acttgataat gaaccgtgat
    17281 gccgtctcct cctaattcat agctgatcac taattcaaaa tcaaatggat acttttctaa
    17341 tgtctccggc gtactcctta agatgaaact agccatttct tgtccatgtt caatgacttc
    17401 aaaatccata tcccgagcaa atccgtgttg ccccatttga tatgttttcc cttgataaac
    17461 atactgatca tcttttaaac gtccaacaaa agggaataaa accggtgcat gtctgcccca
    17521 atacgctgga tcaccttgcc agatatactc taaaccatgt tcttttgatt tcagactgat
    17581 caattccgca ccgtgttcag cgatttcggc gatcagctgg tcattttgaa taataacagt
    17641 cattgccatt cctccaactt cgacttactt tttaaaatgg gaaggctgga acaaacattt
    17701 ctatctgccc cagtcccctt acttctaata actgtgtttt gctaactcgt ctttaaaacg
    17761 ggaattcaat acttttaaat aagttccctt catgcctaac gaacgagatt caatgattcc
    17821 agctgattcc aattttcgta aagcattgac gatcacagaa cgagtgatgc cgatactatc
    17881 ggcaatatta gaagcagtca gacgaccttc atctccatcc aatgcttcaa agatagcttg
    17941 tacagccttc aattcgctat aagataatgt gttgattgcc atttggacag ccgttgcact
    18001 ccgtacatct gcttcgatgc ttcgagactt ctgataaagg atctgcatac cgacaaccgt
    18061 agctccatat tctgctaaga ctagatcgtc atctgcaaac gattgttcca tacgagataa
    18121 gataatcgta cccaaacgtt cgccagctcc aaagatcgga acgacagtcg tcagaccaaa
    18181 tggatatttc tctcttgact ctaccggaaa tgcagtaata tcactagtaa ttgggatatt
    18241 agcttcagtt ttcatcaact gtgccactgt atcagtataa gattcaggaa attgtttctc
    18301 aacaaacatt gttttgattc gttcattgtt cacatcgtga cgttcattat aacctagtaa
    18361 tactccgtca cttcctatga tataggtgtt gctgtcaaga ataccaccta gtgtgacagc
    18421 cattttatcg taaggcaaat ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt
    18481 gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc gtcctccgat tttttataaa
    18541 atatagcgac ttaaatcttc atttgccgca attgagccaa gtttgtcatc cacatatgct
    18601 tctgtgatcg ttatttctcc catttgcata tctggtgctt caaataatag atcttctagt
    18661 agtttttcca aaatggtatg cagtctgcgt gcgccgatat tatctgtgtc acggttgaca
    18721 tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg taaacgtaac tgacacattt
    18781 tctgtaccga tcaacgcgac gtattgtttt actaaagcat tatttggttc ggtcagaatc
    18841 ttcacaaaat cttcagctgt caaatcatct aattctaccc gtatcgggaa gcgtccttga
    18901 agttctggaa ttagatcact tggtttagac aaatggaaag ctcctgaagc aatgaatagg
    18961 acgtgatctg tttggatgat cccgtatttt gtgtttactt gtgaaccttc gacaattggc
    19021 aatatgtctc tttggacgcc ttctctggat acttctccag attgctgtga tttagaagtg
    19081 atcttatcga tttcatcgat aaaaataatt ccactttctt ccgctaattt gattgcctca
    19141 ctatggatat cagcatcctt gactaatttt gctgattctt ccttgatcaa taactctaaa
    19201 gcttctttga ctgttaccgt ccgttccact tttttcttcg gtgtcaaagc acctagagtt
    19261 tcatttaggt cgatccccat ttgctccaat ccattgttca tcattggtgc aggtttcttt
    19321 tggtcttcaa cttcaatggt tacttcacga ctatccagta aacccttttg tagttgttcc
    19381 aagactgtac ggcgattgat tcgaatatct tctgtcactt cttcttgcgg ttcagcggta
    19441 ttttgctgac tagcattaaa aagattcatc atttgttcaa attgattagt ggattgtttc
    19501 tgttcttttt tgataccagg taccaagatt tttaccaaac gtttttctgc atttttttta
    19561 gcacttgtgt atacacggct gtactgctct ttttctacga tttggatcgc attttctact
    19621 aaatcccgaa ccattgattc tacatctctt ccgacataac cgacttcagt aaattttgtt
    19681 gcttcgactt tgacaaatgg tgcattgacg atttttgcca aacgtcgtgc gatttctgtt
    19741 ttaccgaccc ctgtaggtcc gatcatcagc atattttttg gtgtaatatc ttgttgcatt
    19801 ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag caacggcaac agattttttt
    19861 gctgcgtgct gtcctacgat atattcatct aattcactta cgatttgtct tggtgtttgt
    19921 tttaattcat acatatctac tcacctctta cagttcttct acgataatat tatgattagt
    19981 aaagatacag atatctgcgg caatatttaa cgcattttct gcaatttctt tcgcactcat
    20041 gtcgttttga gcatagtttt tcattgctcg ggctgcagcc aacgcaaaat tccctcctga
    20101 tccgatagct aagataccat catctggtgt gataacttct cctgtacctg aaacaagaag
    20161 catttcttta tcattcatta cgatcagcat cgcttcaagt ttttgcatag attgctgtgt
    20221 ccgccattct tgagctaatt cgacagctgc gcgttgtaga ttcccgttat attcgtttag
    20281 tttgccttca aatttttctt ctaaagtaaa cgcatctgca acacttccgg cgaaacctac
    20341 aacaacttct ccattgtaga tacgtcgtac tttctttgca gtgcctttca tgacaacaga
    20401 ttcacccatt gttacttggc catctccagc catagcaaat ttcccgtctt tttcgacggc
    20461 acagatagtg gttgaatgaa atggtgattc gaccatgttt gtttcctcct aaatttgttc
    20521 ttttccttat ttttacgctc ttggatggaa agaacgatag tttttttgta aactttcttt
    20581 tgttacatgg gcatagatct gggtagtcga taaattggca tgtcctagca attcttggac
    20641 tgtccgcaaa tctgccccat tatttaataa atgagtagca aatgtatggc gcagcatgtg
    20701 aggatggata tcactatcca aactgctttt tttgatcatt tgattcaaga catattcaat
    20761 tccagtaggc gtgattggtt ttccatgatg attcacaaaa acgatatcat gctcttcgtg
    20821 atattttgtc atcaatacct ggcggccttc cctgaaatat tcttgcagag catcttgagc
    20881 aaaagaacct agaggagcat atctctcttt gttcccttta ccatggatca acatgacctc
    20941 tgaatcaaaa tcaatgtctg ataccgacaa actgctacac tcacttaaac ggatgcctga
    21001 cccgtaaagg acttccagta aagcgcggtt cctcaaatcc aaaggactat ctcctgctac
    21061 gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt
    21121 ctttttgaga tgaacgtaag aaaatggatt ttcttttatc acttcttctt tgagaagata
    21181 ctgataaaac gaacgtaggc tggctatctt gcgactgata gaatttctgc tatagctagg
    21241 acgatgatca gctaatgcac tcaaatagac acgaaccgta aaatattccg ccttcagcag
    21301 atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc
    21361 ttctttcgtt ttttctgaat atcctctttc gacaatcaaa taacgcaaaa atttttgttc
    21421 ccacgtggct tctgtcattt tctcaccttc cttttgtccc agagaataat ttagcacagc
    21481 gtttataaaa agacaaataa attgtcagaa tttaaccaat atttctaagt ttgttcacaa
    21541 ttggcgcaat tcctcacatt ttgtttgaga aattcttaag aaaaacgcaa gaaaaagagc
    21601 gagactatcc gtctcaccct cttcatggag tttccttact catttgggat tgcagttttt
    21661 tctaattctg tttttattgc ctcattagct tttaaagctc gttgtgcaat tgcttcatat
    21721 cttgcttttt tgtctcgaat gcgctctggc agttcaggaa acagaccata gttggcattc
    21781 atcggttgga aatgttttcc ttccgcatga gtaatgtagt aagccatact tccaatagct
    21841 gtttcttgtg gaaactcgac tggttccatt tcttttgcta agcgtgcggc gttgattcca
    21901 gcaagtagtc cgctcgctgc actttcaaca tagccttcta caccagtcat ctgacctgca
    21961 aagaacagat cttcgcgttt cgttgattgg taagtcggtt tcagcaattc tggcgaattc
    22021 ataaagctgt ttcggtgcat cacaccatag cgaacgaatt cagcattttc taacccagga
    22081 atcatccgga aaacacgttt ttgctcgccc catttcaagt gagtctgaaa gcccacgatg
    22141 ttgtataaag aagctgcagc attgtcttga cgcagctgga taacagcata aggacgtttg
    22201 ccagttttag gatcttccaa tccaactggt ttcataggtc cgaaaagcat cgttttgaat
    22261 cctcgctgtg ccattacttc aattggcata catccttcaa aatatttttc tttctcaaaa
    22321 gatttaagtt ctgccacttc ggcattgatt agtgcctcat ggaaagcttt gaattcctct
    22381 tctgtcatcg gacagttcaa gtaagctgct tctcctttgt catagcggga cttcaaatat
    22441 actttatcca tatcgatcgt agctttgtct acgattggtg cagctgcatc atagaaataa
    22501 aaaccatctg aaccattgaa tgctttgatg ctttctgcta atggatgaga agtcaaaggt
    22561 cctgttgcta ctacggtgat tccttccggt atttcagtaa tttcttcgtt tatcactgtt
    22621 actaatggat gctctttgac ttttcgggta attgtttcag aaaaagaatc gcggtcaaca
    22681 gctaaggcac caccagctgg cactgctgtt tcatctgccg aagtgattac taccgaattc
    22741 aatcggcgca tttcttcttt caataaccct actgcattag ccaaactgtt tcctcttaat
    22801 gaattggaac atactagttc agcaaaatta cttgtgtgat gcgcctcagt cgatttgact
    22861 ggacgcattt catataaacg gacagggact cctgcttgtg cgatctgcca agcagcttca
    22921 cttccggcaa gccctgctcc aacaacattt accattttca atgaaagaac ctcttttcta
    22981 ttatttttgg atgtcttctt cgtaatctcc atttacacag actacttgtt taccgccttt
    23041 gactttcttc tcgaccagat atccgtcaca ttttggacaa tttctgccca ctggtttatc
    23101 ccaagaagtg aattcacatt ctgggaaacg ggaacaacca tagaatagtc tgtttttctt
    23161 agattttcgt tcaatgactt gtccttcatg acatactggg caagtcacac cgatttcttt
    23221 tacgattggt ttcgtattcc gacaatcagg aaaattactg caagcataaa acttcccata
    23281 tttccctaat tttatcacca tcggatgtcc acagacatca caatcgaatc ctgctggttc
    23341 atctttgatt tgaatttttt cgattttttc ttccgcattc gtcagttctt tttcaaacgg
    23401 tttatagaaa cgatcaacca ctttgaccca cgcttccgtg ccttcttcaa ttttatccaa
    23461 atctccttcc atttctgctg tgaaatggat atcgacgatt tgagggaaga attcacagat
    23521 taatgaattg acaatttctc ctagttcagt cggctcaaat cttttttgtg tcaattttac
    23581 atagtagcgt ctttggatcg tatcaagtgt tggtgcataa gtggaaggtc gccctacccc
    23641 gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa cgtgctggtg gttgagtaaa
    23701 atgttgtttt ggttcaatat caacagattg gactacatcg ccttcttcaa gttctggaag
    23761 aatattttct ttctcttctt tgccgtcatc tctaccttcc acatacactt gcatgaatcc
    23821 tttgaatttg atttttgaac cgttcgcaat gaagatgaca ccattttgtt ctaatgtcac
    23881 tttcattgta tctaaaatag ctggtgtcat ctgactagcc acaaaacgtg accagatcaa
    23941 tgtataaagt ttcaattgat ctttatctaa atattgtttg agttcatctg gtgtacgaag
    24001 cacactcgtt ggacgcacag cttcatgggc atcttgtgcg ccttgagcat ttttgatctt
    24061 tcggtgatca tgtctagaaa attcttttcc atacgtatct tcaataaatg ctgcggcctc
    24121 tgcttttgct gtatcggcaa tccgggtaga atcagtacgc atgtatgtaa tcaagccgac
    24181 tgttccttgt ttacccaaag tgattccttc atataattgc tgtgctacca tcatggtttt
    24241 tcttgttcga aagtttaact tacgggcagc ttcttgttgc aggctacttg tagtgaaagg
    24301 attcgcaggg tttcttttgc gctctttctt ttctactttt ttaacttcga agtctgttcc
    24361 atcaattcta gcagtgactt cttttacact ttgtgcatct ggtaatttct ttttcttacc
    24421 gtcgattccc cagaaatttg ctttgaattt cttgcgagct tttttaaaat taccgtcgat
    24481 gctccaatat tcttctggaa caaattctcg gatctctttt tcacggtcaa taataatttt
    24541 taaagcgaca gattggacac gtcctgcact taaccctttc ttgaccttac gccagagaat
    24601 aggactgatc gaataaccaa cgattctgtc taaggtacga cgagcttgct gtgcatctac
    24661 taaatctaca tcgatcgttc ttggttcctt aaaagctgct ttgactgcgt cttttgtgat
    24721 ttcattgaag accacccgat ttttttcttt cagatccaac ccaagtaggt acgctaaatg
    24781 ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc aagtaaactt tttgagcttt
    24841 ttttgctgcg gctttcaggc ttttgatgac atcgcctttt ccgcgtatag aaatataatg
    24901 gggttcataa ttgttttcaa aatcgatacc cattttactt ttcggtaaat ctcttatatg
    24961 gccaacactg gccattactt tatagtttcg tccaagatat ttctcaattg tcttggcttt
    25021 tgcaggtgat tctacaatca ctaaatattt atacgccatt actgaatgga ctcctttcca
    25081 ttgtcgtgtt cctactatta aaagaggatt catacaaatc gtagctaatc atatccattc
    25141 agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg ttattcttta aattctgcca
    25201 agatatcttc acccgaaacc gtgcaaactg caccgtcttg gatcaaatga tgacatccgg
    25261 tagaatgttc tgttaaaata tttcctggaa cagaaaaaac tgttcttcca agatccaatg
    25321 cttgctgtgc tgttatcaga gagccgcttt ttttcttggc ttcaaagaca catgttcctt
    25381 tagacaaagc tgcgataatc cgatttctca ttggaaaaca atgctttttc gggccggatt
    25441 gaggaggata ttcactcaaa agaagatgat catttgcaat cacctcttgc aagtcacgat
    25501 tttcagcagg atagacttgc tctatccctg ttccaatgac tgcgattgtc cgtccaccat
    25561 gagagattgc tgcttggtga ccgtatgtat ctatcccttt cgcgcagcca ctgacgatca
    25621 catatccagc ttctatcact ggttttagta aataataaac tgtttttttg gctaaagcag
    25681 aagtgtgtct tccaccaaca acgcttaaca tgttacaaga aagtaaagat agatcaccat
    25741 tataaaacag tacaattggc ggataggtca agtgaagcag ctgaggggga tatttgggat
    25801 caaagcaagt gataaacgac tgtttcgatt ggattttgcc ccagttagcg tgaatttcct
    25861 gccaactttt gcgaaacagc tcccggtgtt tggttatttt acctatctta atgatgtctt
    25921 caaatccgat ttctttataa ggatactgca tcatccagtt agcaagtgcc cattttcctt
    25981 gactactgat tccttttgta taagcaagtt tcaataaatt ttcttctatt tgatacatca
    26041 taaaaccgcc tttcgtctct ctttctaata agatacgtaa aagcggctgt tttttattgt
    26101 aaatattttt ttacaggtgc aaaactttta cgatggattg gcgtaattcc ttgtcgttca
    26161 agtccttcta agtgttcctt tgttccataa cctgcattat gcgagaaacc atatccagga
    26221 taaagcacgt catattcttt catcaattgg tctcgatata ctttagcaat gatacttgca
    26281 gcagcaatcg acacagatcg tgcatcccct ttgattattt tttcctgctg aataggcaaa
    26341 tccaaggtca tcgcatcgat caacaaatag cttggtacaa tggacaattt ttctactgct
    26401 tggatcatcg ctaatttact tgcttcataa atattcacat catcgattgt ctgctgatca
    26461 acgatcccga tcccgattgc cagggctttt tcttgaactt gttgataaag aagttctcgc
    26521 tttttctctg ataattgttt ggagtcattc agccctaata tgatctcatt tttcggcaag
    26581 ataactgcag ctgccacgac aggtccagct aatggtcctc ttcctacttc atcaatgccg
    26641 gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa agcaaaacat ttcttcatat
    26701 ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa aagaagccag tgcttgctgc
    26761 acgcctttgc gtggatcatt tcttaatgat tctagtgtag gatcatctat tctctgtatg
    26821 cttttcaatc gttctttgat actttggatg gattcagtca tcactttttt gctccatttc
    26881 ttcgcaacga tccagtgtgt atcgtccaag ttttccttga cgaatatcat gcacgactaa
    26941 ttcgctgcca cgatcatagt cttctttata cccacgtttt gctgtaatat ccatcagtaa
    27001 ttccggtgcc aatttttctt cttgctcgat tgataaacca taacgatcgc tgattcttcc
    27061 tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct aaatcatcta aatggacaag
    27121 ctggtctttg atcgcgcctg tcagcgctag ttttttgccg atttcttcat cttcaaattt
    27181 gggccataaa atccctggtg tgtctaacaa ttccaactca ttacctaaac gaagccactg
    27241 ctgacctttt gttacgccag gcttatttcc tgtctgtgca attttcttgc ctactagtcg
    27301 attcatcaac gtagatttcc caacatttgg aatacctaag accattgccc gaatagcgcg
    27361 aggcttgatc ccccgagcag catctcttgc ccgtttttct ttcaatgctt ctttagccgc
    27421 aggtactaga cctttaattc ctttactttc ttgtgccgtg atagctaatg caagatggcc
    27481 ttgtttacga aaatattctt gccacagctt tgtctgttgt ggatctgcca gatccgcttt
    27541 attcaataaa acaaggcgag gtttttgctg aatgatctga tctagcattg gattacgaga
    27601 agataaaggt aatcgtgcgt ctaccaattc aaatacgatg tccacatatt tcattttttc
    27661 agaaacttct cgtcgggctt ttgccatatg tcccggaaac cattggatgg tcattgttct
    27721 ctccattcct ttcataccgg aatattcccc ggctttactt gtttttttct ttttcaatat
    27781 aaattcactt ttttattata aagaaattcc atctattagt taagtgaaag tgaaccagtc
    27841 ctcattctat cacatcttca cttttttgat gatgcaaatt tttattttgg tttctatata
    27901 tgaatctaaa tttttgacag cgtttcctaa ataagagatg atagacgaac aaaacagaaa
    27961 ggagacgaaa caaatgaata gtatggtttt tgttaatttt cctgttgctg atgtcaatcg
    28021 ttccgttgct ttttatgaaa agctgggttt taaaaaaaat gaagaatttt ctactgaaga
    28081 agctggttca atggtatggg atgacacttt ttggataatg cttctctctc atgattttta
    28141 taaaaaattt ataaaagata aagaaatagc tgataataaa aaaacaagtg gtgctttgat
    28201 ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt gctcaaattg caaaagaaaa
    28261 tggtggtact ttccacgaag tagatatggg aatgcctgaa gaagaaatgt tttcattaga
    28321 agtccaagat ctagacggaa atacattaga acccgtatgg atgaagatgt aagcagataa
    28381 ctaatctgac gttttttata acaaaaaaac aaaggcggat gatacgataa gtattctccg
    28441 cctctatttt ttttattcgt acaatctctt ttttacagtt tataaaagca gtcttctcac
    28501 ccttttttta attgttagat gccagtgtat gtaaatcacc aatgtgtcat gacacattgg
    28561 tgattttatg gtatcctttt attattaact gacaaaggag agaatatttt gaacactttg
    28621 attgcaaaag acgtttttag attgcccgaa atgaatgcaa gattgttgtc gacctctgct
    28681 cttttattag cccttgatgt catcttatac aagatagcga ttggtcctgc ttattttcaa
    28741 atcacattcg gatttttaag tatggcgctt atgggttatc tatatggacc gatctgggct
    28801 ggtttgcttg aaatgttgtc gaacacttta aatttcacga tatttggttc aggaacgttt
    28861 caagctgctt ttcttatcac ggctttcctt ggtggattta ttaatggctt atttttatat
    28921 cataaaaaga taaacctgcc ttccgtcatc gctgcccaat tattgattat gatcccagtc
    28981 agccttttta tcaattcttg gctgatgtct gttttgtttg gtgcagattt caaagtaatt
    29041 tttctaggta gattcatcag gaatatgatc caaatcccta ttcaaataat ggttgtttat
    29101 tcatttatca aaactttaga ggctagaaaa gtgtttaaac gagctgtttg attttacgta
    29161 ctaaggtagt aaactgattt tggattaaac aataaagcgt gcagtctcaa cagactgcac
    29221 gctttattgt ttctcactgg atccatttta tttcatcaag tggataatag acaaatcttg
    29281 ctttacctag aatatcagtt ttattgatcg tgccaaacga acgactatct tttgaaactc
    29341 ttcgattatc tccaagtaca aaataattat cttccggaag cttggattgt cccgttagct
    29401 cttgtagtgt aaagtccgtc gtataggatg catttgcatg atctgatttc aggtttttag
    29461 ttagataagg ttccttaact actttcccat tgatcttcaa ttgatcattt tggtagctga
    29521 cattttctcc tggaagaccg attactcgtt tgatataaat cgtgccatct gctaattgaa
    29581 aaacaacaat atcaaatcgt ctgatctccg aaaatttttc catcacaacc atatcacctt
    29641 gcttcaacac gttttccatt gaattgcctt ccactggtac cgggatgaat aaaaatcccc
    29701 gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt gaatacaagc cagaagcgat
    29761 taatatagcg ttgttttttt gtcaatctct atcaatccat ccctcttttt ttcattcttt
    29821 ctttattata agaaaaatat caaaaaaatg cgactagata gctgaaataa aaataattct
    29881 ttcaattagg ctattggaca aaaaactttt ttgatcataa agtacaaaac tatctcttat
    29941 atgtcatgat tattttctaa atatttttcc catgcttcgt caaagatatc catggacggc
    30001 aaatagtcga cattcagttc cagataatta gaaatttctt cgtagctaga gctgtgctta
    30061 ggaaattgga tgtctttacc ggcttctgta gcgaaaacag ataaagcatc atgtgtgcca
    30121 ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg ttcctccaat
    30181 tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt aaaattttca ggatgctttt
    30241 tataatactc ttgatggtct tcttctgcta gataaaatgg ttgggctggt tcgatttttg
    30301 taacgattgg ctgttcgaaa cgtccacttt cagctaactg tttcttactt ttttcagcaa
    30361 tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg ataattgtca ccgcgatcct
    30421 cgaattgtcc gaaagcgtca gttggatcag tttgctgcca gtaaagttct actaattgtt
    30481 catatgaaat caatgttgga tcatagataa tctccactgc ttctgtatgt ccagtcgttt
    30541 ggctttttac ttgttcataa gtcggatctg gtacatgccc tccagtatat cctgacatca
    30601 ctgtatggat accaggaagc gtgtcgaaag gctggatcat acaccagaag cagcctcctg
    30661 caaatattgc tcgttccatt atgattcccc ctctaatact actcatccga tgatttttta
    30721 ttttcttttg tcttgctttc agtactgctg gtgctagttt tgttggtatt tgagttgttt
    30781 gaagcatcag aggtatcttt tggcaaatat agactaaacc gaatgtcatc atttactaag
    30841 tcgattttat ctgctttaat atacatcccg ttttgcattc ggaattgatc caacctaagc
    30901 atgatagtca gatcatctgt attgacttcg atccaatctg gtaatttata gtttctcttg
    30961 atcatattca tcacatcttt gattggcaaa ctcaatgttc cgatagaaag acttctagct
    31021 tttagttgga catttccgtt ttccatcaca tacggatcaa aatataaata gaaatcgata
    31081 ggaaaaccta gtacttcgaa agtaccattg agtagtgctt cattttccaa ataaaattta
    31141 tattcaatat cactatcttt ttgatactca ctaaggaaaa agctgatgat ttgattgact
    31201 ttttctttat ttgaattgat cgttaagaca ggatctcctt gtctttccgt aagagctggt
    31261 atttctggga gttctggttc ccgattagca aaaatacggc tgcctaaaaa tacggtcgaa
    31321 ccaagtacta ccccaagaag gataaggaag gcgaatttcc agtaatttgt tttctttgtt
    31381 ttgtttgaag atacgtcaga tcttgttttc atgtttattg atcatcctcc ttctctaacc
    31441 attggttttg tgtctcgatc agctgatctc tcactgcatt tgccatcaat tgataaccta
    31501 aattatttgg atggaaatga tcttcttcat agagcacatt atttttgatg ccatttagat
    31561 tttctgtcgt gctagttgtt tgatctgttc cttcttctgt gacacctact tcctgattca
    31621 atccttgata aagcaaatca ttgattggga taaagtagca gttcggcatt ttttttgtca
    31681 ccgtttctgt tcctttgttc caattgttaa caattgtctg catgtcagtg atctcaggaa
    31741 agttcaaata aaatggattg tagatgccta aaatgtaaat aggtgcggtt ttattgaggg
    31801 atcgaaattc ttccaatagt tctgtgacat tttcctgata gtttttcagt ggctttttaa
    31861 aggaattgac cgttaacccg aataaattat tttggataac tttcatcaaa tcattaccgc
    31921 caacagtcag tgtgataaaa tcagctgttt tgatattgtt tcgaatttct tcttttgttt
    31981 ttactcgctt taagatctgg tcacttcgtt caccatttac accatagttt tctgtttcaa
    32041 ccgcggtcaa attataacgg tcttttagat catttgcgac aaggggaacg aatcctcctt
    32101 gcttggtttg atctcctact ccttcagtca gcgaatctcc gatagctatg tagtgaagtt
    32161 tttctttttt atttggatct tctctcacag tagttactgg tttcattagt gggccagctt
    32221 tcggtataga agcagctaaa cctgcataga tgatgatcgc aattaataat gggaacaaaa
    32281 cgaggatcaa atgtcgtttc gttaatttca ttttttcacc tcttgtattc gattggttct
    32341 aatgttatca aaaattttat caaaaaggta gtctttatga aaaaagaaaa caattatttt
    32401 cctaatttta taaagaaaaa gagaataagc tgcaagcctt tcaggctgag ggcttatcct
    32461 cttttcgact aatctacata atacatgatt gcaaacgcgt ctttaccagt atgcgtagcg
    32521 ataaccggat tcgtatgcag tacagggata tgcatgtccg ggaataattc ttgcaaaccc
    32581 tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg aaataccgat ttgtcgcaca
    32641 tttggcaatt ctttcagttc atttttcaaa ttattaaacc atttatcgaa cgttttcttt
    32701 cctcgcccct tagttacagg gattaattca gaatgggcga aattcatcac cacgcgtatg
    32761 ttcaaaacac tagacagcaa accagttgtt cggctgattc ttccaccttt taccaagtta
    32821 tctagtgtgg atattccgat gtataatttt gtattgtctt gtactttttt gacttcttca
    32881 aggattttct ctactgtttt accttctttt gctaattcag ctgctcgaat tacctgaaaa
    32941 gataatcctt ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt tgttagatta
    33001 ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg gatcgaaatc
    33061 acttcactgc cgtcttctcc tagctcatca tacatcgaga tgaattctcc aattggtggt
    33121 tggcttgttt tgggcagatt ttttgctttc gccatcattt ccataaatct ttcgccgtct
    33181 agttggtcat catcgggata tactgttccg tcgatcatga tggatagcgg catgacacga
    33241 atgcctaaag catcgcgtac atttttttcc atcgtacatg aagaatctgt tacgattttt
    33301 acatttgtca ttattttctc tctctctttc tttaacaaga tgccttattc atgttaaact
    33361 acatgtaaga caatttctat ttttagtata acagacaaat aatgattttt cattaattac
    33421 acctagtaaa ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt atgaaatatt
    33481 aaatgaagtg tttaatgcag tcacccatgg aattgggact ggattgagta tcgcgggact
    33541 agtggttctt ttagtaaaag gtgcccactt gcattcctcc ctccatattg tctcttatgc
    33601 tatctatgga tcgatgatga ttcttttatt tttaacgtct actttgtttc attcattgat
    33661 ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat agttcgattt ttcttttgat
    33721 tgcaggaagt tatactcctt tttgtttgct gagtatccga ggatggctag gttggacttt
    33781 atttgtatta atctggttgt ttgcgattct tggcattata tataaagcgt tgacgttgca
    33841 taaacgggag agagtatcaa aagtatctac gatcatctat atttttatgg ggtggctctg
    33901 tgtcgtggct gctgttcctc tttatcactc tcttggaatg actggtgtta ttttgatggt
    33961 agcaggcggg ctttcttaca cgataggcgc attcttttat agcttgaatt ctatccgcta
    34021 tatgcatgtt gtttggcatt tgtttgttat gttaggtgca ggttttatgt acttctctgt
    34081 tttattcttt acttaataaa aaaaataaag ctatggatgg gacagctcta aataacagct
    34141 agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta caggattctc
    34201 attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcca tttctccttg
    34261 actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag catctccatc
    34321 gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa attcttgata
    34381 aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca gagcaagcac
    34441 gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat ctctcgtcaa
    34501 aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaatg tttttcgtcc
    34561 catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat ttggcaaacg
    34621 ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc aaattgcagc
    34681 taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc gccggatgtg
    34741 ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac tgcttttcct
    34801 gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct ttcatttgtt
    34861 cgatatgatt gatataaaga tgagcatctc ctaatgtgtg aacaaattcg ccaaccgcta
    34921 aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg ttgaacggaa
    34981 cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt ccctcttgaa
    35041 cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca tcttccggat
    35101 tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct tcgatcactt
    35161 gtttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat tgatagccgt
    35221 agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg atcttctcgc
    35281 aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc aaacatctat
    35341 ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag cgttcaaatg
    35401 cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc gtatctcctt
    35461 ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc gttgttaaca
    35521 aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt aatgtgcctg
    35581 tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct aagtcaagat
    35641 acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt attcccaccg
    35701 tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca attcttgtaa
    35761 tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga ttttttcttc
    35821 aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt aagataattt
    35881 cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag caggctgctg
    35941 ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt cttgttttcc
    36001 gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac ggtcatggga
    36061 aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg tcaatgttgc
    36121 tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct gaatcagcag
    36181 tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca gtgtcccatg
    36241 cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt gactgccatc
    36301 tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt tatctggtgc
    36361 catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga tagacattgt
    36421 accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt ttcccgcacc
    36481 atttttacca gtaataccta gtctttcccg agattgaatc aataaatcta aatgttctag
    36541 cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt ttttccctaa
    36601 acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt acaagttctc
    36661 ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg cctgtacgcc
    36721 agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt cttgttctac
    36781 ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc ctttatactc
    36841 gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta gaaaataacg
    36901 atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt ccaaccactc
    36961 gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat cgggttcatc
    37021 aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag tactgatttt
    37081 tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg tatctgtagt
    37141 ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt gcacatcagg
    37201 gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa cagtttggat
    37261 caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg aaaattcagc
    37321 tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt gccgatcacc
    37381 gtcgctgcta tcgataccgg caataatatt taagagactt gttttacccg tcccgttcgt
    37441 cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt caaacaaatc
    37501 tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca tgttccctcc
    37561 ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt tggtatttgt
    37621 tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga tatgttttgt
    37681 tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt tttttgactt
    37741 tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg tataagattc
    37801 taacaaattg atattcctgc gatagctgtt cgcagaaaac gtcgtgattt tttcagcaac
    37861 ttcgtattca tcaatgattt ttctttcaag ataataacct cttagcattt ttttcgtaaa
    37921 cagactatct tgctggatca tccacgaaga aatcaaattc cccgagttca tttttattcc
    37981 ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt tcatcataaa tatcagtcaa
    38041 gttatctaaa ctttcaataa tcaactgagt atttttttga tatgtctctc tgatttcttg
    38101 gatatcttca tcactaataa atgtctgacg atcagcatat cttctttgcc aaaacatttt
    38161 cggatggaga acgattcgca ttaacctacg gacaacaatg aaccaaaagc cgataaaaga
    38221 caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt aaaaacgata
    38281 accattagaa gaaagcttgc ccgaacgata actttcatcc aatccatccc gttcgatcga
    38341 caaaatcagt gcttgtattt cttgtacttc ttgcttttca tcatcggtta aatcttccaa
    38401 gtacagatca cgtatccgtt cttgataatt ttctatcact gcttcagtgg ctaaaaattc
    38461 ttttgtatta cgatcaattt cctttatttc ttttcgcaaa acttcaacga cctcttctaa
    38521 aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct gtttccgtca gcataggcaa
    38581 gacaatgatc cctatgacca aagtcactaa gatgacaccc gccgtcagaa acaaaagcaa
    38641 agaccgctgg taaaacatca tattattgat tgaaaatggc aaaataaaaa tcgtagccaa
    38701 actgactgtt ccctttacac cgccaaatgt taagatcaag atctcgttca actgtttttt
    38761 gaacttttta ctgccatctt tgaatacata aaaaagactg atagaaatga atctgatcac
    38821 aaaaagcatg acagagatta atacgataat cgctaataaa agaccgtttg gatataaacc
    38881 gtctccccat actggcgaga acacttgcgt caattcgatt cctaaaaata taaatactaa
    38941 tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg acaattctgc
    39001 atcaaacaca gagattttcc taaaaccaga agcttggaga atacctgctg ccactgcagc
    39061 aatgattcca gatacatcaa atgcctctgc caatacatag gctagaaaag gaagcaaaag
    39121 ttcgatcagc agataccctg tgacatcttg tgctgatgct ttttcgatca aatgaattat
    39181 tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct cccccaatac tagaaactac
    39241 tagactcatc cccgcgttta ctgctgaaaa agatccggtg atcagcgcac caagagcaaa
    39301 ctggaaggca gtaacaccag atgcatcatt caaaagacct tctccttcca aaatatgcat
    39361 cgccttttta gggatattca ctcgtccaga aagagaactg actgctaccg catctgtcgg
    39421 acctaacgct gctccaaaag caaagcaggc agcaagtggg acacttggca acaggaatga
    39481 taacgtcgca cctaccccga caagggtcaa aatgactcct ccaaatgcca agaacaaaat
    39541 tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt tctccttctc taaataacaa
    39601 tggtgcaata atcataacta agaacaattc aggttcaaaa tcgattgatt gtccccactc
    39661 tgtcagacct aataccactc caaggaaaat ttggatcagt ggagctggaa tcattggcaa
    39721 aatgcggcta gctatgttcg ataaggtgat cgttactgca aatacaataa ctaactcagc
    39781 aaattccatc gtccatcccc tttatttttt attttgtgta agcattttta aaaattgttc
    39841 ttttttcttt aaaattttgg tttcgactac ttcaacctgt gtttggttcc cctctaattc
    39901 acactgtaca cgttttgact ccaaacgaag cagatcatgt ttcagacgtt ccaactgacg
    39961 atacatcaat atatttcttt tacgcaactt acaaagtatt tgacggcggt catccatcga
    40021 aagggcgcca tagcgatcat gcaacagttt tttttgttgt tcaaaactct ctctcatttt
    40081 ccagatctcc tatacacaaa cttccatcat tatagcaaag ttctttttgg atgaaaaata
    40141 tttcattgtt tcatcgttta ttgctagtta gaaatttcca taaatttttc aacgcctcat
    40201 tctattccaa atagtatcag atatcaagta aaacttaaga aagataaaag tttctgacaa
    40261 aaggatgttt actttcccat tcctgccttt cttgttcttt tatcacaaca aaaaaatggg
    40321 gctgcgacaa aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc
    40381 ctatcagtag taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt
    40441 attcgaaaaa ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca
    40501 agttacctgt tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc
    40561 ctttatctag atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca
    40621 ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta
    40681 ggttgatatc atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag
    40741 cacacggatg gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt
    40801 gtttgatcca aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa
    40861 aataaaattt tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt
    40921 tattcttgta ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt
    40981 tcttctaatg acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta
    41041 gtgtttttta aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt
    41101 ttattctttg ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac
    41161 caggtttttt tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg
    41221 acttgatagg taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa
    41281 aatagatcat caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga
    41341 atggttccaa agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt
    41401 taatcatttg gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta
    41461 gatccagttg gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg
    41521 gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt
    41581 agcactctgt ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct
    41641 tgatgaattc cacatttata cgttctacag atattttacc taatagctcg tggttttctt
    41701 tgatggcgta gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc
    41761 cgcgcatcat tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca
    41821 agatctgttc ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt
    41881 tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa
    41941 cgaattcgac atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg
    42001 tgatctcata ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat
    42061 ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca
    42121 catcatggat tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt
    42181 catatcctgc attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta
    42241 attgttttaa tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact
    42301 cagttgcatg gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc
    42361 atcttgttta ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca
    42421 tgtttttttc aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa
    42481 cgaataaaac atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc
    42541 ttttttctcc ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc
    42601 gagccatctg agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca
    42661 cttcctgctg catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat
    42721 cttaccaaaa ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg
    42781 ctacatttag gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg
    42841 aattcatatg gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag
    42901 tatttatgca tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt
    42961 ctcagggatc accttgatct taagagcttt atttggtatt gatccagctt atacgacttt
    43021 attaatcaac atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc
    43081 ctatactgtt ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc
    43141 actagaaatc aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc
    43201 aggtgtagga agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat
    43261 cgctcggatt ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga
    43321 tatcatcgtc ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt
    43381 gattgtttcg tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc
    43441 aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg
    43501 tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa
    43561 aatcatctat atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga
    43621 gattgacgaa aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt
    43681 tacttatttg aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata
    43741 ggctatgagg aaagattcca catccagctg acctttgtct tttttagtac tatactattt
    43801 ttaactaatt gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct
    43861 ttcggttaac ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt
    43921 gatcgatttg agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc
    43981 gttcaattaa ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt
    44041 cttctaagat agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat
    44101 ataaaggctt tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt
    44161 cttttccctg caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca
    44221 gtcctcgatc cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat
    44281 gattggcaag ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga
    44341 ccatccacgt taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc
    44401 tgttaggtca tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata
    44461 agtcaataat tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc
    44521 tttcaagaaa tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc
    44581 aacggtaaag tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt
    44641 agcagtttct tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa
    44701 gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt
    44761 cagtaaaagt tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag
    44821 gtgataaagt tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc
    44881 ttcttcaatc atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata
    44941 atgcggatta acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa
    45001 ttgaagattc gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc
    45061 ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa
    45121 acgttcctgc atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata
    45181 aaccactgct gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg
    45241 gatcaatggt tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc
    45301 agggattccg ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc
    45361 actttgctta tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat
    45421 tgcaatttct gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg
    45481 ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc
    45541 agctaatata tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga
    45601 caagtcttca tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc
    45661 ctcttatctc actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt
    45721 ttggatgttg gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt
    45781 tttctgccaa aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt
    45841 ggattggata gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag
    45901 gatatgcttg gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag
    45961 cagaaagtag atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat
    46021 ttgacaggtt ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa
    46081 acggccgagt cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac
    46141 gatgatcaca aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca
    46201 cctgccgctc gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca
    46261 gcactacacg atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag
    46321 aacggatcga tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa
    46381 aacctgccag atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt
    46441 cgggatagcg tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag
    46501 tgttggcaca aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat
    46561 ctgcaaacaa atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa
    46621 gttctcttag ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta
    46681 cttgataatc aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa
    46741 gcaaattcta gaaaagataa gagcaaagct cacctcataa cttgcaaata aataaaaatt
    46801 aaataaaata catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt
    46861 tattcctttt ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc
    46921 tttttcaact gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta
    46981 ctttgctagc tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg
    47041 cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta
    47101 aagcatcttg aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta
    47161 aatcagtagc tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca
    47221 cctcctccac aagagatatg atagaaatat catttagtta aaactttctg agtggtccag
    47281 aagtttaaaa agaaatattg attaacttca atattctgtt atcacgatat catatgaacc
    47341 gcttaatagc aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca
    47401 aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct
    47461 cctacgaggg ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt
    47521 ttctaagaaa cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa
    47581 tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc
    47641 tgttggtgtt ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga
    47701 gatacgaaga ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg
    47761 ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc
    47821 acgaatctct tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt
    47881 attcacgacg ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc
    47941 ttgttcccga atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat
    48001 ggcacgcatt gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt
    48061 acgcatacca gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt
    48121 gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa
    48181 aatcgcattg atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat
    48241 cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat
    48301 atcaccaatc cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga
    48361 ataaaattca tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat
    48421 cactggttta ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc
    48481 ttcgcgtccg ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg
    48541 atgcttgatc tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac
    48601 gctgaattct cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg
    48661 agtatcttcg acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc
    48721 gacgttcggg cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa
    48781 gtttccaatc tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat
    48841 taaacgaagt atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag
    48901 acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat
    48961 cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa
    49021 atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag
    49081 ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct
    49141 ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat
    49201 gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa
    49261 aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt
    49321 ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt
    49381 cttgttcagg attgatagac aacactttga catttacatc atcattgaca gccaatacat
    49441 cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc
    49501 cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat
    49561 ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta
    49621 ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc
    49681 tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg
    49741 cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa
    49801 caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt
    49861 tagcatctaa acggcgtttt gaaagtagat agctgccgtt ttctttatct ttgccgatcg
    49921 tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta
    49981 ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc
    50041 cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt
    50101 ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat
    50161 caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt
    50221 tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa
    50281 caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac
    50341 agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc
    50401 atcctctgct tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg
    50461 tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa
    50521 acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg
    50581 taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt
    50641 tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc
    50701 ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg
    50761 ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc
    50821 ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat
    50881 ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact
    50941 tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct
    51001 ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc
    51061 agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt
    51121 gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga
    51181 accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc
    51241 ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga
    51301 cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt
    51361 tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga
    51421 agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata
    51481 agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa
    51541 tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc
    51601 gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc
    51661 ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag
    51721 tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt
    51781 ccccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt
    51841 gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct
    51901 aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg
    51961 aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac
    52021 tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg ctctgtggca
    52081 tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa
    52141 tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg
    52201 cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc
    52261 acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac
    52321 tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca
    52381 ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct
    52441 ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt
    52501 tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat
    52561 tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa
    52621 tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt
    52681 cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca atgagcctca
    52741 tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc
    52801 aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt
    52861 ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt
    52921 aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct
    52981 tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata
    53041 gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat
    53101 gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct
    53161 caatagatag atttgataca aacgaaattc tccataatct aattgtccat ctacattcca
    53221 ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc
    53281 tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg
    53341 atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc
    53401 attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga
    53461 atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg
    53521 gatacatctg ctttgttgca gataaaccgc tgactcatca ttgttttttg ccagctgata
    53581 aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc
    53641 ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc
    53701 acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt
    53761 ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa
    53821 tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt
    53881 atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt
    53941 ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc
    54001 ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat
    54061 aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt
    54121 gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga
    54181 tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt
    54241 tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc
    54301 caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa
    54361 gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg
    54421 aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc
    54481 ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac
    54541 tagttttttc acggacccta taggacccat ttatattatt tcaacttttt cagccagttt
    54601 tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct
    54661 cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac
    54721 tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta
    54781 tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg
    54841 tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg
    54901 acataacgac ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt
    54961 ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc
    55021 attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa
    55081 ctttctggct ctacgagagc accaggataa aaaataagta atggagactt tccatgattc
    55141 tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact
    55201 tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg
    55261 agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc
    55321 caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa
    55381 taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat
    55441 atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat
    55501 aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa
    55561 agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa
    55621 aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc
    55681 ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa
    55741 attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat
    55801 ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga
    55861 tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca
    55921 cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa
    55981 aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg
    56041 catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat
    56101 cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag
    56161 ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg
    56221 gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat
    56281 ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc
    56341 ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag
    56401 catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg
    56461 atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat
    56521 tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc
    56581 cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta
    56641 gcttttcact gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat
    56701 gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg
    56761 attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct
    56821 aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg
    56881 acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa
    56941 cactcactga atggagctat tgacttatcc acgtgcttat cgatagccgt ttatcggttt
    57001 cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac
    57061 acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca
    57121 ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg
    57181 tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc
    57241 gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga
    57301 agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac
    57361 acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa
    57421 tccattgttc ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat
    57481 caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt
    57541 gattgctact acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc
    57601 acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca
    57661 gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact
    57721 gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt
    57781 gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg
    57841 gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata
    57901 tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc
    57961 catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc
    58021 tagacagcct acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc
    58081 tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc
    58141 gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt
    58201 tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat
    58261 aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt
    58321 ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat
    58381 atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc
    58441 tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc
    58501 aataataata aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg
    58561 ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt
    58621 ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa
    58681 aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt
    58741 cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat
    58801 ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa
    58861 acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag
    58921 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
    58981 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
    59041 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
    59101 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
    59161 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
    59221 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
    59281 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
    59341 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
    59401 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
    59461 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
    59521 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
    59581 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
    59641 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
    59701 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
    59761 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
    59821 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
    59881 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
    59941 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
    60001 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
    60061 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
    60121 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
    60181 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
    60241 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
    60301 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
    60361 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
    60421 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
    60481 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
    60541 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
    60601 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
    60661 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
    60721 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
    60781 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
    60841 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
    60901 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
    60961 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
    61021 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
    61081 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
    61141 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
    61201 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
    61261 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
    61321 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
    61381 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
    61441 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
    61501 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
    61561 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
    61621 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
    61681 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
    61741 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
    61801 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
    61861 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
    61921 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
    61981 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
    62041 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
    62101 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
    62161 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
    62221 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
    62281 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
    62341 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
    62401 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
    62461 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
    62521 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
    62581 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
    62641 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
    62701 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
    62761 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
    62821 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
    62881 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
    62941 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
    63001 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
    63061 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
    63121 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
    63181 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
    63241 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
    63301 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
    63361 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
    63421 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
    63481 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
    63541 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
    63601 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
    63661 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
    63721 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
    63781 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
    63841 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
    63901 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
    63961 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
    64021 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
    64081 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
    64141 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
    64201 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
    64261 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
    64321 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
    64381 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
    64441 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
    64501 ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg
    64561 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
    64621 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
    64681 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
    64741 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacgta
    64801 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
    64861 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
    64921 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
    64981 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
    65041 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
    65101 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
    65161 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
    65221 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
    65281 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
    65341 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
    65401 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
    65461 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
    65521 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
    65581 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
    65641 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
    65701 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
    65761 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
    65821 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
    65881 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
    65941 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
    66001 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
    66061 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
    66121 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
    66181 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
    66241 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
    66301 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
    66361 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
    66421 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
    66481 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
    66541 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
    66601 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
    66661 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
    66721 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
    66781 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
    66841 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
    66901 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
    66961 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
    67021 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
    67081 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
    67141 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc
    67201 ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc cacgcttatt
    67261 ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt aatgctttag
    67321 cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact tcttccaatg
    67381 ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct tcccgatcag
    67441 tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct gcgtgttctg
    67501 ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca tcttcttcac
    67561 cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct cggacttttt
    67621 ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt ccaaggtctt
    67681 gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata tgaactggaa
    67741 tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga atccaccatg
    67801 tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact gccttcatca
    67861 gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct acataacgtt
    67921 tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc gcttcttggt
    67981 cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa agggagacac
    68041 ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg gttggcgcag
    68101 aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag ctgtgaattg
    68161 atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt tggatcaatt
    68221 tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat aaatcatcat
    68281 atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct gcttcatacg
    68341 tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct atttggcttg
    68401 ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt
    68461 tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat cagctaagcc
    68521 agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt cttctgccac
    68581 cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa
    68641 aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata aaaaataaac
    68701 ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat tacggacaga
    68761 aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt gcgtcaaagg
    68821 tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt cctgaggttc
    68881 ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta tcgtctctct
    68941 gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga ttggtgaaac
    69001 aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt tttcattatc
    69061 taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt cgcgcccatg
    69121 agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat caggatcaag
    69181 cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa gcaattcaat
    69241 ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt
    69301 cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca tagaagctaa
    69361 tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt cgaacaacaa
    69421 aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata aaacttgtct
    69481 tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt tttcctgccc
    69541 tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag aattacgaag
    69601 tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa
    69661 caatccagag cgttctaaca attcatttga aattgattca ttcttgaata cttgctgaag
    69721 aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt ctaaagacaa
    69781 cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca atagcatgtg
    69841 atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag cagattcagg
    69901 atttcggaca gctatagtac gctgacgcca ttgatcttct aacggtatct gttcgaagtc
    69961 tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa gaaaagtaaa
    70021 cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga
    70081 aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt taccagactt
    70141 tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc gtactgtttc
    70201 gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat tgatatcttt
    70261 tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact aactgaaatc
    70321 agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc attgcttttt
    70381 caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc agtaactgat
    70441 tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc atgaatacaa
    70501 agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca tggaacagca
    70561 ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc aatgggttga
    70621 agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag taatacatca
    70681 aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac accgcaatga
    70741 tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta ggcaaataac
    70801 ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga tacccttcaa
    70861 tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt gctgtaacgg
    70921 cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg cctgagactt
    70981 tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat gtagtaaaat
    71041 cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct tctgccatag
    71101 caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg cctgtcattg
    71161 caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc tgcggactca
    71221 ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct tcagtaattt
    71281 gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg atattggaga
    71341 cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca tgaagataag
    71401 ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga gcaaccggct
    71461 caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc aacataagta
    71521 cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct tcttccacca
    71581 aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc gcgccccatt
    71641 tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa agctcaaaat
    71701 aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga tacgtcccca
    71761 taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc tggatactca
    71821 ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca tgggccagcg
    71881 tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg gttacccctg
    71941 tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct aacaaagctg
    72001 ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt gcttccaaaa
    72061 atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact ttgcgaatcg
    72121 aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc aagcggacag
    72181 cataattatc tgcacctact cggataactt cagccatgac agaaccgctt gtcaaaaagc
    72241 ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa
    72301 gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt atctgttctc
    72361 ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc gattgttctt
    72421 taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc cgtacaagat
    72481 ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata attgctaaat
    72541 acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca aagttcagaa
    72601 aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt gatgcatctt
    72661 cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc
    72721 cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg tgtttaagtc
    72781 tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa aacacacgaa
    72841 tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct gtttttatta
    72901 tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc tagtgctgta
    72961 ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc cgcgtaatcg
    73021 gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg gaatagcttg
    73081 ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac acaaagaatc
    73141 ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc gtcagccatc
    73201 gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg gacgatcata
    73261 cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc gacttgcccg
    73321 tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt aacaaccatg
    73381 atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa cgcaggaaca
    73441 gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc acctaataaa
    73501 aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc aacaggcaga
    73561 aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc
    73621 tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa gataaatcct
    73681 gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt gtaccccact
    73741 tcttgatttt ccattttttg ccttttttcc actatttgcg tactccttct ttcatttaat
    73801 tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat aggtctctat
    73861 tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat tatgatagag
    73921 atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt tcccttcctc
    73981 atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt ctactgtgtt
    74041 ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat atgcttggac
    74101 agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg ccaaatattt
    74161 gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc catgcagact
    74221 ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata ttatcgcctt
    74281 gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta tttctatttg
    74341 atagctttcc actcgtttta ccgaacgata agttcttcct cataaaaaca acaccgcacc
    74401 aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc gttttcgctt
    74461 catttcatca cctagttatt ttgacatctt tttttatctt actatttctt tatcttcttg
    74521 gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt
    74581 tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa tgactgatat
    74641 aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat
    74701 gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc tctttttact
    74761 gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac tcgaacgcta
    74821 ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg aagcatctat
    74881 gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg aaaaaatcga
    74941 tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat ttgccagcag
    75001 cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg ttaaaggcgt
    75061 tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt tactctcttt
    75121 atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc atgacatcgg
    75181 gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa
    75241 agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa aagcttcctt
    75301 tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac gcttcaatcg
    75361 agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg atttatttgt
    75421 taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca atctttttga
    75481 tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact ctatctatcc
    75541 agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta atgctattga
    75601 ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat cggatgcttg
    75661 aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat acgatcaaca
    75721 atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta tatggacaaa
    75781 agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt ttgatggctg
    75841 gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca gttgctcctt
    75901 ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct gtcgtctttt
    75961 tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa actcaaggac
    76021 agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc ctttctgttg
    76081 gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc attgctgctt
    76141 cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat gtaatgatga
    76201 ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct
    76261 atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc ttcaaaaata
    76321 tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca acagaaattg
    76381 cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta ctttctgcca
    76441 ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca ttgaaatttc
    76501 attttattat ccaatttcct agtatactat tgggatttct tttaatcatt atgggcagag
    76561 ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt gttctagctt
    76621 tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg tctattctgc
    76681 ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc tattcatggg
    76741 agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac atcgttattg
    76801 gggtatacaa tctgccggat ttccctcatc ggcgtcacca ttttatttcc tttttcttat
    76861 ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt agtttcatga
    76921 tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa gcctttaatg
    76981 aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt caattgatct
    77041 tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact gttcttctcc
    77101 aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt aagaaagaag
    77161 attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc tatttaccag
    77221 ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc tacgatttca
    77281 ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca gggaaaaaaa
    77341 tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca tttgatgtct
    77401 tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct gataattggt
    77461 tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat tatctacaaa
    77521 gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt gcaacgatca
    77581 tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat gattctaaaa
    77641 aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat atgaaagaag
    77701 aacatatcca atggttcaat ttagggatgg cccctctttc aaatgtcgga acttccagaa
    77761 agagtttttt acaggaaagg attgcatact tggtttacga gtttggttct catttttatt
    77821 ctttccatgg attaagagaa tataaaagca agtacgctac aaactgggtc tcacgttata
    77881 ctctatattc tagagatagt tggattgcat atgttatgat tgctttgctg attattgata
    77941 atgcgccagt tgagcagaca caaggtagaa taagtggttt gaaaaaatgg atacgaagaa
    78001 agtattaaaa actgcatacc gatagatgaa cactctcatg atgaataaaa gagactgaag
    78061 taaaacgaaa gttcatgctt ctacaatctc ttcttataca attgaagaac aaagagcggt
    78121 ccaaacagaa atcctgtttg gaccgctctt ttattttaaa catttttcat taccaaaata
    78181 ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc
    78241 gccttcacga attgagaatg tcgttccgtt ttcaacagcg attggatgga tcaagtcaac
    78301 atcaatcgtc acgttgtcac caggcatgac catttctgta tcttctggca atgtgattgt
    78361 tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga tagttgttga agaatggagt
    78421 atgacgtcca ccttcttctt ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg
    78481 agtaatggaa cctggtttag caagtacttg tccacgttcg atatcgtcac gttggatccc
    78541 ccgtaacaac acgcctacgt tatccccagc ttccccgtaa tctaacgttt tacggaacat
    78601 ttctacacct gttacaactg ctttttgtgt ttcaggtttg atccctacga tctctacctc
    78661 atcaccgaca cgaacagcac cacgatcaat acgacctgag gcaacagttc ctcgacctgt
    78721 aattgagaag acgtcttcca ctggcaatag taatggttta tctgtatcac gttctggtgt
    78781 tgggatatat tcatctactg tatccatcaa ttccataata gcagcttcag catctggatc
    78841 gccttgcaat gcttttaatg cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc
    78901 atattcgctc aataactcgc gaacttccat ttctaccaaa tcgatcaatt cttcatcatc
    78961 gactaaatca actttgttca agaaaacaat caggtatttt acaccaactt ggcgtgacaa
    79021 taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc
    79081 accatccatt tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc
    79141 atcaatatgc gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg tattgatcgt
    79201 aatcccacgt tcacgttctt ctggagctgc atcaatacta gcatagtctt gaggatttgc
    79261 taggcctttt ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc
    79321 aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt ctgtcataat gttcttttgc
    79381 cataatttac aaacctccta aataatatag atcactgaaa aatttcagct ctcacctttt
    79441 tattatagtc tttttttgac taaatcaaaa agaatacgtc cgacttactt tttgtaagat
    79501 tcgaactaag cttattatac gtcaatattg gtcaaagtca actaagagca ctcgtctgtc
    79561 ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt gttttttgag aaaaacttca
    79621 agatagtcct taactttctt ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc
    79681 agctgttgcc aacaactttc aatagacgaa atcttctatt tcgttaataa taaggaaaga
    79741 ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt ttaggcatat ttctcggcgg
    79801 aagttttgca gcttatcatt atttttacgg cggcgaagct tattatacaa aaatcacgac
    79861 gaatggcgaa atgtcagcta cccaagcaga taacggagag aaattcacga cttataccta
    79921 caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag
    79981 agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt
    80041 attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga
    80101 taattagtaa atggtcttaa aattttatta gataaaa
//
LOCUS       DASJRQ010000002        76169 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJRQ010000002 DASJRQ010000000
VERSION     DASJRQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.002"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..1490)
                     /locus_tag="U4A80_000076"
     CDS             complement(69..1490)
                     /locus_tag="U4A80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1973751.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="U4A80_000077"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="U4A80_000077"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1973752.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="U4A80_000078"
     CDS             complement(3901..5157)
                     /locus_tag="U4A80_000078"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1973753.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="U4A80_000079"
     CDS             5737..7038
                     /locus_tag="U4A80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1973754.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="U4A80_000080"
     CDS             complement(7107..7706)
                     /locus_tag="U4A80_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1973755.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="U4A80_000081"
     CDS             7961..8842
                     /locus_tag="U4A80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1973756.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="U4A80_000082"
     CDS             8855..10465
                     /locus_tag="U4A80_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1973757.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="U4A80_000083"
     CDS             complement(10542..11201)
                     /locus_tag="U4A80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1973758.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12149)
                     /locus_tag="U4A80_000084"
     CDS             complement(11484..12149)
                     /locus_tag="U4A80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1973759.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="U4A80_000085"
     CDS             complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="U4A80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1973760.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="U4A80_000086"
     CDS             complement(12929..13939)
                     /gene="dhaK"
                     /locus_tag="U4A80_000086"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1973761.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="U4A80_000087"
     CDS             complement(13914..14360)
                     /gene="dhaM"
                     /locus_tag="U4A80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1973762.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14463..14783
                     /locus_tag="U4A80_000088"
     CDS             14463..14783
                     /locus_tag="U4A80_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1973763.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14895..15764)
                     /locus_tag="U4A80_000089"
     CDS             complement(14895..15764)
                     /locus_tag="U4A80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1973764.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15860..17164)
                     /locus_tag="U4A80_000090"
     CDS             complement(15860..17164)
                     /locus_tag="U4A80_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1973765.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17161..17670)
                     /locus_tag="U4A80_000091"
     CDS             complement(17161..17670)
                     /locus_tag="U4A80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973766.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17845..19980)
                     /locus_tag="U4A80_000092"
     CDS             complement(17845..19980)
                     /locus_tag="U4A80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1973767.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20063..21718)
                     /locus_tag="U4A80_000093"
     CDS             complement(20063..21718)
                     /locus_tag="U4A80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1973768.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21720..22034)
                     /locus_tag="U4A80_000094"
     CDS             complement(21720..22034)
                     /locus_tag="U4A80_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1973769.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22204..22464
                     /locus_tag="U4A80_000095"
     CDS             22204..22464
                     /locus_tag="U4A80_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1973770.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22478..22792
                     /locus_tag="U4A80_000096"
     CDS             22478..22792
                     /locus_tag="U4A80_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1973771.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23522..24076)
                     /locus_tag="U4A80_000097"
     CDS             complement(23522..24076)
                     /locus_tag="U4A80_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1973772.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24202..26131)
                     /locus_tag="U4A80_000098"
                     /pseudo
     CDS             complement(24202..26131)
                     /locus_tag="U4A80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26437..27153)
                     /locus_tag="U4A80_000099"
     CDS             complement(26437..27153)
                     /locus_tag="U4A80_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1973773.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="U4A80_000100"
     CDS             complement(27154..28977)
                     /gene="glpO"
                     /locus_tag="U4A80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1973774.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="U4A80_000101"
     CDS             complement(28991..30487)
                     /gene="glpK"
                     /locus_tag="U4A80_000101"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1973775.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30623..32083)
                     /locus_tag="U4A80_000102"
     CDS             complement(30623..32083)
                     /locus_tag="U4A80_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1973776.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32073..33368)
                     /locus_tag="U4A80_000103"
     CDS             complement(32073..33368)
                     /locus_tag="U4A80_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1973777.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33700..34377)
                     /locus_tag="U4A80_000104"
     CDS             complement(33700..34377)
                     /locus_tag="U4A80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1973778.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34407..35498)
                     /locus_tag="U4A80_000105"
     CDS             complement(34407..35498)
                     /locus_tag="U4A80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1973779.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35599..36033)
                     /locus_tag="U4A80_000106"
     CDS             complement(35599..36033)
                     /locus_tag="U4A80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1973780.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="U4A80_000107"
     CDS             complement(36617..38074)
                     /gene="ascB"
                     /locus_tag="U4A80_000107"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1973781.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38071..39966)
                     /locus_tag="U4A80_000108"
     CDS             complement(38071..39966)
                     /locus_tag="U4A80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1973782.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40024..41799)
                     /locus_tag="U4A80_000109"
     CDS             complement(40024..41799)
                     /locus_tag="U4A80_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1973783.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41849..43345)
                     /locus_tag="U4A80_000110"
     CDS             complement(41849..43345)
                     /locus_tag="U4A80_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1973784.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43539..45161)
                     /locus_tag="U4A80_000111"
     CDS             complement(43539..45161)
                     /locus_tag="U4A80_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1973785.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45489..46802)
                     /locus_tag="U4A80_000112"
     CDS             complement(45489..46802)
                     /locus_tag="U4A80_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1973786.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47047..47271)
                     /locus_tag="U4A80_000113"
     CDS             complement(47047..47271)
                     /locus_tag="U4A80_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973787.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47291..47713)
                     /locus_tag="U4A80_000114"
     CDS             complement(47291..47713)
                     /locus_tag="U4A80_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1973788.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(47855..48565)
                     /locus_tag="U4A80_000115"
     CDS             complement(47855..48565)
                     /locus_tag="U4A80_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973789.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48583..49080)
                     /locus_tag="U4A80_000116"
     CDS             complement(48583..49080)
                     /locus_tag="U4A80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1973790.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49117..49569)
                     /locus_tag="U4A80_000117"
     CDS             complement(49117..49569)
                     /locus_tag="U4A80_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1973791.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49704..50309)
                     /locus_tag="U4A80_000118"
     CDS             complement(49704..50309)
                     /locus_tag="U4A80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1973792.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50759..51379)
                     /locus_tag="U4A80_000119"
     CDS             complement(50759..51379)
                     /locus_tag="U4A80_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1973793.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51381..52250)
                     /locus_tag="U4A80_000120"
     CDS             complement(51381..52250)
                     /locus_tag="U4A80_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1973794.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52273..52680)
                     /locus_tag="U4A80_000121"
     CDS             complement(52273..52680)
                     /locus_tag="U4A80_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1973795.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52699..53988)
                     /locus_tag="U4A80_000122"
     CDS             complement(52699..53988)
                     /locus_tag="U4A80_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1973796.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(54015..54317)
                     /locus_tag="U4A80_000123"
     CDS             complement(54015..54317)
                     /locus_tag="U4A80_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1973797.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(54342..56435)
                     /locus_tag="U4A80_000124"
     CDS             complement(54342..56435)
                     /locus_tag="U4A80_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1973798.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(56924..57688)
                     /locus_tag="U4A80_000125"
     CDS             complement(56924..57688)
                     /locus_tag="U4A80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1973799.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(57799..58668)
                     /gene="mdcB"
                     /locus_tag="U4A80_000126"
     CDS             complement(57799..58668)
                     /gene="mdcB"
                     /locus_tag="U4A80_000126"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1973800.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(58693..59769)
                     /locus_tag="U4A80_000127"
     CDS             complement(58693..59769)
                     /locus_tag="U4A80_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1973801.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(59793..60386)
                     /locus_tag="U4A80_000128"
     CDS             complement(59793..60386)
                     /locus_tag="U4A80_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1973802.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(60390..62066)
                     /gene="mdcD"
                     /locus_tag="U4A80_000129"
     CDS             complement(60390..62066)
                     /gene="mdcD"
                     /locus_tag="U4A80_000129"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1973803.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(62056..62355)
                     /locus_tag="U4A80_000130"
     CDS             complement(62056..62355)
                     /locus_tag="U4A80_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1973804.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(62457..64103)
                     /gene="mdcA"
                     /locus_tag="U4A80_000131"
     CDS             complement(62457..64103)
                     /gene="mdcA"
                     /locus_tag="U4A80_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1973805.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(64110..65021)
                     /locus_tag="U4A80_000132"
     CDS             complement(64110..65021)
                     /locus_tag="U4A80_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1973806.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            65141..65998
                     /locus_tag="U4A80_000133"
     CDS             65141..65998
                     /locus_tag="U4A80_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1973807.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(66185..66547)
                     /locus_tag="U4A80_000134"
     CDS             complement(66185..66547)
                     /locus_tag="U4A80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1973808.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            66893..68332
                     /locus_tag="U4A80_000135"
     CDS             66893..68332
                     /locus_tag="U4A80_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1973809.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(68339..70891)
                     /locus_tag="U4A80_000136"
     CDS             complement(68339..70891)
                     /locus_tag="U4A80_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1973810.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(71005..72336)
                     /locus_tag="U4A80_000137"
     CDS             complement(71005..72336)
                     /locus_tag="U4A80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1973811.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(72302..73780)
                     /locus_tag="U4A80_000138"
     CDS             complement(72302..73780)
                     /locus_tag="U4A80_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1973812.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(74085..75920)
                     /gene="tdc"
                     /locus_tag="U4A80_000139"
     CDS             complement(74085..75920)
                     /gene="tdc"
                     /locus_tag="U4A80_000139"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1973813.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        21786 a        16166 c        13054 g        25163 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
    11461 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
    11521 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
    11581 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
    11641 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
    11701 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
    11761 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
    11821 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
    11881 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
    11941 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
    12001 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
    12061 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
    12121 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
    12181 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
    12241 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
    12301 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
    12361 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
    12421 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
    12481 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
    12541 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
    12601 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
    12661 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
    12721 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
    12781 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
    12841 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
    12901 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
    12961 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
    13021 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
    13081 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
    13141 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
    13201 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
    13261 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
    13321 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
    13381 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
    13441 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
    13501 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
    13561 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
    13621 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
    13681 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
    13741 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
    13801 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
    13861 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
    13921 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
    13981 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
    14041 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
    14101 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
    14161 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
    14221 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
    14281 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
    14341 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
    14401 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
    14461 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
    14521 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
    14581 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
    14641 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
    14701 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
    14761 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
    14821 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
    14881 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
    14941 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
    15001 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
    15061 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
    15121 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
    15181 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
    15241 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
    15301 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
    15361 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
    15421 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
    15481 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
    15541 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
    15601 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
    15661 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
    15721 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
    15781 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
    15841 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
    15901 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
    15961 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
    16021 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
    16081 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
    16141 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
    16201 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
    16261 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
    16321 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
    16381 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
    16441 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
    16501 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
    16561 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
    16621 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
    16681 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
    16741 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
    16801 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
    16861 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
    16921 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
    16981 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
    17041 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
    17101 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
    17161 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
    17221 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
    17281 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
    17341 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
    17401 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
    17461 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
    17521 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
    17581 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
    17641 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
    17701 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
    17761 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
    17821 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
    17881 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    17941 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    18001 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    18061 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    18121 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    18181 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    18241 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    18301 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    18361 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    18421 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    18481 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    18541 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    18601 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    18661 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    18721 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    18781 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    18841 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    18901 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    18961 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    19021 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    19081 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    19141 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    19201 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    19261 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    19321 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    19381 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    19441 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    19501 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    19561 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    19621 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    19681 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    19741 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    19801 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    19861 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    19921 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    19981 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    20041 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    20101 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    20161 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    20221 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    20281 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    20341 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    20401 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    20461 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    20521 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    20581 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    20641 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    20701 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    20761 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    20821 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    20881 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    20941 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    21001 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    21061 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    21121 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    21181 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    21241 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    21301 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    21361 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    21421 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    21481 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    21541 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    21601 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    21661 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    21721 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    21781 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    21841 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    21901 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    21961 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    22021 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    22081 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    22141 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    22201 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    22261 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    22321 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    22381 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    22441 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    22501 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    22561 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    22621 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    22681 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    22741 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    22801 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    22861 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    22921 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    22981 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    23041 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    23101 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    23161 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    23221 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    23281 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    23341 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    23401 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    23461 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    23521 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    23581 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    23641 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    23701 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    23761 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    23821 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    23881 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    23941 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    24001 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    24061 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    24121 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    24181 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    24241 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    24301 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    24361 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    24421 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    24481 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    24541 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    24601 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    24661 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    24721 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    24781 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    24841 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    24901 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    24961 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    25021 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    25081 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    25141 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    25201 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    25261 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    25321 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    25381 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    25441 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    25501 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    25561 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    25621 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    25681 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    25741 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    25801 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    25861 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    25921 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    25981 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    26041 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    26101 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    26161 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    26221 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    26281 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    26341 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    26401 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    26461 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    26521 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    26581 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    26641 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    26701 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    26761 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    26821 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    26881 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    26941 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    27001 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    27061 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    27121 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    27181 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    27241 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    27301 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    27361 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    27421 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    27481 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    27541 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    27601 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    27661 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    27721 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    27781 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    27841 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    27901 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    27961 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    28021 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    28081 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    28141 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    28201 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    28261 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    28321 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    28381 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    28441 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    28501 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    28561 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    28621 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    28681 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    28741 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    28801 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    28861 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    28921 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    28981 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    29041 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    29101 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    29161 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    29221 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    29281 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    29341 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    29401 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    29461 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    29521 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    29581 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    29641 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    29701 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    29761 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    29821 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    29881 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    29941 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    30001 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    30061 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    30121 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    30181 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    30241 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    30301 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    30361 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    30421 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    30481 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    30541 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    30601 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    30661 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    30721 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    30781 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    30841 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    30901 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    30961 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    31021 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    31081 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    31141 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    31201 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    31261 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    31321 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    31381 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    31441 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    31501 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    31561 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    31621 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    31681 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    31741 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    31801 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    31861 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    31921 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    31981 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    32041 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    32101 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    32161 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    32221 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    32281 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    32341 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    32401 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    32461 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    32521 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    32581 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    32641 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    32701 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    32761 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    32821 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    32881 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    32941 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    33001 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    33061 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    33121 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    33181 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    33241 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    33301 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    33361 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    33421 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    33481 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    33541 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    33601 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    33661 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    33721 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    33781 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    33841 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    33901 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    33961 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    34021 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    34081 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    34141 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    34201 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    34261 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    34321 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    34381 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    34441 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    34501 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    34561 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    34621 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    34681 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    34741 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    34801 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    34861 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    34921 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    34981 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    35041 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    35101 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    35161 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    35221 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    35281 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    35341 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    35401 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    35461 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    35521 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    35581 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    35641 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    35701 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    35761 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    35821 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    35881 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    35941 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    36001 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    36061 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    36121 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    36181 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    36241 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    36301 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    36361 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    36421 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    36481 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    36541 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    36601 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    36661 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    36721 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    36781 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    36841 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    36901 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    36961 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    37021 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    37081 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    37141 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    37201 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    37261 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    37321 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    37381 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    37441 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    37501 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    37561 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    37621 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    37681 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    37741 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    37801 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    37861 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    37921 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    37981 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    38041 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    38101 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    38161 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    38221 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    38281 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    38341 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    38401 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    38461 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    38521 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    38581 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    38641 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    38701 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    38761 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    38821 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    38881 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    38941 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    39001 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    39061 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    39121 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    39181 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    39241 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    39301 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    39361 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    39421 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    39481 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    39541 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    39601 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    39661 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    39721 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    39781 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    39841 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    39901 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    39961 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    40021 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    40081 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    40141 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    40201 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    40261 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    40321 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    40381 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    40441 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    40501 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    40561 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    40621 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    40681 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    40741 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    40801 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    40861 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    40921 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    40981 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    41041 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    41101 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    41161 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    41221 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    41281 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    41341 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    41401 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    41461 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    41521 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    41581 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    41641 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    41701 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    41761 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    41821 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    41881 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    41941 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    42001 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    42061 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    42121 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    42181 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    42241 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    42301 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    42361 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    42421 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    42481 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    42541 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    42601 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    42661 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    42721 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    42781 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    42841 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    42901 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    42961 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    43021 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    43081 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    43141 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    43201 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    43261 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    43321 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    43381 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    43441 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    43501 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    43561 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    43621 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    43681 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    43741 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    43801 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    43861 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    43921 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    43981 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    44041 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    44101 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    44161 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    44221 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    44281 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    44341 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    44401 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    44461 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    44521 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    44581 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    44641 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    44701 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    44761 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    44821 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    44881 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    44941 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    45001 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    45061 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    45121 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    45181 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    45241 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    45301 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    45361 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    45421 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    45481 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    45541 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    45601 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    45661 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    45721 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    45781 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    45841 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    45901 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    45961 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    46021 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    46081 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    46141 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    46201 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    46261 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    46321 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    46381 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    46441 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    46501 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    46561 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    46621 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    46681 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    46741 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    46801 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    46861 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    46921 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    46981 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    47041 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    47101 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    47161 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    47221 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    47281 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    47341 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    47401 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    47461 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    47521 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    47581 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    47641 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    47701 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    47761 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    47821 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    47881 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    47941 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    48001 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    48061 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    48121 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    48181 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    48241 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    48301 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    48361 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    48421 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    48481 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    48541 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    48601 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    48661 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    48721 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    48781 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    48841 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    48901 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    48961 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    49021 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    49081 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    49141 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    49201 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    49261 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    49321 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    49381 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    49441 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    49501 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    49561 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    49621 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    49681 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    49741 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    49801 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    49861 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    49921 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    49981 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    50041 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    50101 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    50161 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    50221 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    50281 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    50341 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    50401 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    50461 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    50521 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    50581 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    50641 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    50701 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    50761 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    50821 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    50881 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    50941 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    51001 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    51061 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    51121 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    51181 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    51241 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    51301 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    51361 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    51421 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    51481 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    51541 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    51601 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    51661 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    51721 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    51781 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    51841 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    51901 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    51961 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    52021 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    52081 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    52141 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    52201 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    52261 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    52321 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    52381 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    52441 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    52501 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    52561 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    52621 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    52681 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    52741 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    52801 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    52861 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    52921 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    52981 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    53041 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    53101 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    53161 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    53221 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    53281 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    53341 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    53401 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    53461 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    53521 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    53581 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    53641 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    53701 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    53761 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    53821 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    53881 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    53941 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt ttttaccctc
    54001 ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat tttttcttca
    54061 atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg atcaactgcg
    54121 aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg tttcatggaa
    54181 actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt gttcaaaaca
    54241 tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa
    54301 atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc aactactgaa
    54361 aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc caacttctct
    54421 tcgttgttga tcaattcaat tagttctttc atgatgttca gatgcgagta ggagtctact
    54481 gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa atcgattggt
    54541 tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg tcgggcatgg
    54601 gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac tgcacgaatc
    54661 atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct agcagctagt
    54721 tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct gtccgtcaaa
    54781 atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt tgtttcgaat
    54841 aatttttcta acttgccata gatgattcga tctactcggc caccactttc tttcatgaca
    54901 tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa ccgctgtagt
    54961 tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt taaaatcgga
    55021 tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa aaccagattg
    55081 acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt aatctcgatc
    55141 tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat cccatggtta
    55201 cagacaacga ctgatttata aacataattc aagtctcgct tgattacact ggcgatcgtc
    55261 gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc aattacttct
    55321 tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt tgttcggatg
    55381 ttttctttca atggattcat gacttggata tgattcttga ttcttacaat cagtgcagcc
    55441 atgtgcttat agagattttc gaataaagct tcttccttta gatggaaagg tacgtgtgtc
    55501 tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac ccactcgatt
    55561 gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata atttctttct
    55621 acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag
    55681 ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc atgtttcctg
    55741 attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat atcatcttct
    55801 tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttccac caaaggaaaa
    55861 taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat aaagaagtcg
    55921 gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga tcgttctgaa
    55981 cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata actgaccagt
    56041 gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg aaattcttct
    56101 tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa
    56161 atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt ttctaatcga
    56221 gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt acggatctcc
    56281 ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg gatcgtccgc
    56341 tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg ggctgtcatg
    56401 attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa
    56461 gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa gtggaaagaa
    56521 agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa cgaaaaagac
    56581 atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg tgaaaaagct
    56641 gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg
    56701 ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagag gttgtgacag
    56761 agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca tattttacaa
    56821 aatcgtgcct tattgccgat aggctatctc tggcacaccg tttattcaga aaaaggagct
    56881 gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc agcataatcg
    56941 gctgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa taccatatct
    57001 gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct tttcgatcga
    57061 ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg tggatctgtc
    57121 gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca ccgtctgctt
    57181 tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa cagcttctgg
    57241 ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc ttttggattt
    57301 ccagaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat atctccgctt
    57361 tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc cctcctactg
    57421 acaaaacagc taatgcaaga aatcccgttt gctcataact agaaggacct gtacctggac
    57481 cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg cccccatatg
    57541 aacgaccacc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct ccaccatgac
    57601 cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc actaacaaaa
    57661 tgattgctaa ataattgatt tttttcattg tcttcctcgt tatcttttat tacaaaaaac
    57721 tatttttatg atagccttgc taaaaggaag agtcaataac cagatgcata aactgttttt
    57781 ttagcaattt tgtcttattt agtcattgag taacatcgga caaatgtatg tgtcggcagt
    57841 ccataattcc tctatcttca gcatgaacaa gcttgcagac aagagatctg cgcttcctcc
    57901 aggagagatt tggttttcct gacaaaagtg caacagtgct gtaaatggac gatttggtaa
    57961 agcactttga ctagcagcat gagcaagttt ttgcatttcc actaacgttt tctgatcact
    58021 gcgatgcaat acgcatgtgt catctagcga actcatcaaa actaacaaca tatgcatttg
    58081 tttctgtaca tatgaatcat tatctagctc cttagctgct tgaattgcaa gcctgatatg
    58141 cggataccct aatatcgctt cttcatatgc accatttacg ccatattttt gcttcaccct
    58201 atgaccatga gtggtttttt tatgacgata ttttgtatca ggaaaagcag caagcttgct
    58261 gacatcatca agtattttcg ataaagaaaa aggctgttcc atactgattt tttgtgcaat
    58321 gacactgatc aatagcccca ttgcccaaat cgctccttta tgggtattga tgccatttgt
    58381 agcaatcagc attgcctttt ccgcttttcg cccaatctca gcaattcttt ctcttaactc
    58441 ctgatcaata gcatgatgcc atgctatttg agccatttct tcaaaaaaag gagttagcgt
    58501 cttagcagac ttaagaaata aagcatagtc catatcggaa tgtgcacccg catccagtgc
    58561 atccactaat ccaggttttg gacttaatgt tacttctgcg atcaatgctt cctctaccaa
    58621 cgcagcgagt tgctgagtca ctgctttttt tcgttcgtca tttatcatga cactcactcc
    58681 ttcttcctac tattacttgc tgaaaccttt tttgatagct acccaaatga tatgaagtaa
    58741 ccagcttaaa gcaaatgcac aacttgccgt cattagataa gctacaagaa tcatgactcc
    58801 tgaaggtaac tgcagccaac tatctttaaa caataataac agtacttgga aaacaaatac
    58861 attggctaaa tacgtacgat aagcataaac agacaaccat ttgaacaatg gcaattttgg
    58921 cgaatcgttt acaatcattt tggaagcgcc tatgaagaca gcaaagatga tgactaaact
    58981 gtataaactt tggatcgtat tcaagtaagg tgcattcgca aagctcaagt ctcctggatg
    59041 tgcgagcaat gaacggacag aaaagaacgc gataactaag gcaacaggta agaaaagata
    59101 acgtactctg tccaaatatt gatagacttt ttcgtgataa acaaatgcgg ctaccccata
    59161 aatcatgaaa ctagcaacaa accgatctaa taagtaccag ctgtcatgat aaggcccagt
    59221 caatacttgt gtttgataga acagatacca tgcgacatat aaaatcgttg aaccaatcaa
    59281 tactggccat acatattttt tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg
    59341 catgaacaat tggatctgca gcatcataac ggtataccat aaatgaggcg ctccatcacc
    59401 tgtgacatat ttcagtaaaa agcttgcagt atccgtatac ggagtttgtt gttggactgc
    59461 tggaaatagc caaagataag ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa
    59521 cttttctttc aaaaattccc aataagacaa atgctgactt gtttttacca tattataaac
    59581 aattgcaaaa ataaacattg gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa
    59641 agctgtattt gttgcgctgt ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat
    59701 aacagccgtt acagctgata ctttctggaa gtcacttacg ttgattttga tttttttgtt
    59761 ttcaaacatc tctttctctc tctttttctt tattaccaaa gttctttggt taatttaggt
    59821 ccttcgactg ttttcaatag aatttttttc tctggctctc tcaaatactc ttttagtgaa
    59881 aaaccgcctt gccgagtcac tacttgggta tcgaattgct caaaaaaagc aggatgcgat
    59941 tgaattacct gaattggcaa ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca
    60001 ctggtttctt ttactgccgg aatcccagtc gataattcaa atcctaagct gccgccgatt
    60061 ccccatttac atcctgataa tatgtttcta gcagcatgaa attgctgata aacaggataa
    60121 tccatgattt tctcttgtct atacgattct tgttcgatga tgtcccacgg atgaatgact
    60181 tgtttgacgg catgttttga gatttctgtt gaccatctct gattgcgggc aggtccacgt
    60241 aaaccaatcg gtagtatttc tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc
    60301 ttttcagtta gaacgaccca ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa
    60361 aggacaatat catgtgcttg aatcattttt tatgcatccc attcttcgtc cattttggca
    60421 cggactttat ttgtttcgac acgtccgaat gttttggctg tttcgtttgt gtatcgattt
    60481 gtcagcatcg tgtcatttgt attgcggaca tcttgaatgg cttcattgat tgcttcgatg
    60541 actttgctga cattttgttc tgaagcagct tggtcggtga tatcttctag gaaatgatag
    60601 atcgctccta gttttgtata attattgata tcgtaagcga tagctggaac tttttcagca
    60661 gctgcttcga tttcagcaac cgaacgtttt gtcacacgtg cagcactttc tttagacatc
    60721 gcttggacgg taatttcttt attgtttaaa gcaatcaagc ggcttgattg taatccatgt
    60781 gctaaaaatg cacctgaaat agcatttcct gcaatcaatc caataactgg atgtccagct
    60841 tgacggagtt ttgcataagc agcagcacta ttagccaaag ccacatggat accgatcaat
    60901 tcttcgttat agccgtatgc ttggcttggt acatcgatca caacgatgat tggccgtttc
    60961 acttttttgt ctttatcttc ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct
    61021 tcaactaaac caacttcacc gtttcgtaca cggtacatcg ggtttttagg atcaggtacg
    61081 attgatgtca cgatggcttc ttctatgaat ttacttgatt taccataata cacagtacta
    61141 atagaagaag ttggattttt catgccagtt agtgccgcaa accaatcgta accaacactt
    61201 gctgcagctg gttcatttcc ttctgctact tctggtaagt caattgattt tgcttgatgg
    61261 ttagcaattg cttcattgta atcgtcaatg gccattggtt ttgaaagatc taaagaatca
    61321 agtaatgata aataaaatgc tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt
    61381 gctgcgatga cagcagcttt gattgcttct acactatcag tcactaattc atcaatgatt
    61441 tttgattcta cccgttgacg agatcctaat gtattccaaa tcaagtcttt gtcactagaa
    61501 tcaaattctc gaacgcctgc ttcttgttca ataacttctg gtccattcaa accgactctc
    61561 gctttttttg ttgcgattaa ataactcatt aatgctgaag taatcgacat accgccaaat
    61621 gatcctacac gtcctggaat caaaccgatg actggtacat attgttttag agcaatgatt
    61681 acatttccga tttctgaaat agataacagt ccgtagtttg cttcttgcaa acgaacacca
    61741 cctgtatcta gtacaatgac tggaaagatc tcatttcctt ttttattttc ttctaacaca
    61801 tgttcaagtg cagcaacgat ttttgcaccg gaaacttcac cgataccgcc accttggaat
    61861 tttccttcca tagcgattac tactgcttgt ttgccattga tcgtgccacg gctgacaacg
    61921 atcccgtcat cactttcagg gacgattcct tgtgctggta aacttggcgc gatgatttga
    61981 tcgaatggat caatcaattc acgtccattg tcaagtagag cgttcactcg ttctctggcg
    62041 tttaattcta caaaactatt tttcatctga aagttcctcc attgcttgcg tcaatcgtag
    62101 gtacacaact cctggtgtag caccgaagtc attgatcacg atatctgctg aaattggata
    62161 acgatcaaaa aatctcgtta atacattgtt ccatacttcg ccgaagccat cgcttcctgt
    62221 acaaattttt acagtagatt ctgttttttc tgttggaaac atcaagattt ccaaatctcc
    62281 tgaaccgacc actcctacat ggattttctt attgatcggc tgtttcgttg gatatttata
    62341 agctaattgt tccatggatc tctccccttt ttgtaaaaca gagcatacac gttctgctta
    62401 ttcgtctttc ttcttccatc tgggtaaatg gcgacaaaag gtttttctcg ccaattttat
    62461 gaccaagttc tgaatttcat tggtggttca tacaggccat ctgaccattc gatcaaatca
    62521 tcgatggttt gtgcagctaa taatgaacgt ttcgcttcac tacgtttgat gcctagatct
    62581 tctggtaatt tcacgatgcc ggctgcacgc aagctatcta gtgattcttt cgtgctagtc
    62641 attccaagag gtgtaacacc gccaattgct gcaattgctg cttgtcgttc ttcttttgtt
    62701 tttgctttgt acaaataagc aattccttct tctgtcacga tatgtgtagt atcttcgcta
    62761 taaatcatca ctggtgcatt ggctaagcct gcttgttttt gaacagctag tgcatctaat
    62821 tcttcgacaa agactggttt tttatttgaa ccatatgttt caaccatttg aacaactaat
    62881 ttttggcctt tccctaatgg atcatcattg tcgcgaagtg attgccatgc tggtgtagaa
    62941 tgacgacgac cgccaggatt atgtcccatg ttaggcgcac cgccaaaacc agataaacgt
    63001 ccttttgtta cggtagaaga gtttccttcg taatctaatt gcaaggttga accgataaac
    63061 atatcgatgg catattgtcc tgcagcttgt gataatgttc ggttactgcg catcgtgccg
    63121 tctttaccga caaaaaagac atctgggcga gcagcaatat atttttccat accaacttcg
    63181 ccaccaaaac tatggatgct ttcaacccaa cctgattcga tggctggaat catcgttgga
    63241 tgtgggttca atacccagtt cttcgcgatt ttccctttta accctagtga ttcaccataa
    63301 gttggcagta acaattcgat agctgctgta ttgaatccaa taccgtgatt caaagattgt
    63361 acgccgtgtt ctgcgtagat tcctttgatt gccatcattg ccattagaat ttgcaattct
    63421 gtgatgtttt gaggatctcg tgtgaataaa gcttctaatt gataaggttt atctgctttc
    63481 acgacaacgt ctacccagtc accagggata tctacacgtg gtaaagaatc gacaacttcg
    63541 ttaacttgaa cgatcacgat accatctttg aaagctgcag cttcgatgat tgttggtgtt
    63601 tcttccgtat tgaaacctgt gtacaagttg ccgtctttat ctgctttatc tgcagctact
    63661 aaaacaacat ttggaatcaa atcgatgaat aagcgtccat aaagttccaa ataagtatgg
    63721 atatcaccta atttcattgt gccgtcttca atcatttggg cgacacgcaa gctttgtgca
    63781 ccagcataag agaaatcgat cttgcttgcg attccttttt caaaaatatt caaatgttct
    63841 ggtcgtgaga tactagacat gatcaaatgc agatcattca ctttttctgc atctactttt
    63901 tcaagacttt ctgaaagaaa acttgcttgt ttttgattgt cgccttctaa tacaactttg
    63961 tcgccgggag caatcagttt ttccattact tctacgattt tttcagtttc tacatacttg
    64021 ccattgagca agttgctgat tttttccaat cgctgcgctt ttttttcgcg tttagaagcc
    64081 cagttgcggt ttggtttttc cataattagt catctccatt tttgatatag aaacgtggcg
    64141 tcatcaatac catgttggtc tagattgatc gtacggatcc ctttatctac accaaatttt
    64201 gtatgaatca gctttgtcaa agtattgccg gggggaaatt caatcgtcag atccattcct
    64261 aattcttttg ccaagtccat catttggttc cactgcacag ggaaagctag gttttccaac
    64321 aaatctttac gaatctcttc tgcgtcctga gtggctcttc catccgtatt ttttatatac
    64381 aaacatctag catcagatag atggacagac tgcatatacg gcttgaattt cttgaccgta
    64441 tcattcatca agagacagtg agatggcaca gggactttca gcagcttcgc tgtttgcgct
    64501 tggtgccgtt tggcttctgc caacgtaacg gataaacctt cgatactccc agataacgta
    64561 tgttgcatcg gacaattttc atttgacaga taaactggat ggtcaggatc aaatgttttt
    64621 gctacaattt cctcagcttc gcttctcgtc acacctacta ctacgcccat tgcataaccg
    64681 cttggataag cttctttcat taagactgcg cggtgataga ctagttccgc tgcattctca
    64741 aatctcaaag atcgacaagc aacagctgca caaaaagcac caatcgaatg tcctgctgca
    64801 atatcaggct gaataccgtt tttttccatt tcatctaaat aaaatactgc ttttgtcagt
    64861 aatgctagct ggataaaaac cgtatcttga tagttttctt ctttatcaac taaccgatac
    64921 cccgtcacat tttctaaacg ttccatgtac atttcaggga tatccgctaa catccctaac
    64981 ttctgactgc cttgtccagg aaataagaaa cctgtactca tgagcctcgc ctcctcagca
    65041 cttacttttt ccactgctat cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag
    65101 aaaaaaccat aacaaaaagg tatggatggg atggataatt atgaaattgc ttcatctgca
    65161 gtattttaaa aaagtattcg aaacaaaaaa tgttacccaa gcagccaaag aattatttat
    65221 ttctcaacct gccttgagcc gtgcgatcaa acatttggaa aacgaattag gtgtcccttt
    65281 gttttaccat agcggcagaa acattgagac gaccgtttat gcagaagaat tttatccata
    65341 tgcagtcaag acattagaca cattagaaga aggaatcaaa ttattgtctt cggtcaatga
    65401 gaagatcgtt acttccgtca tattgtattt agaggtagcc tctgtttcta ttccgaactt
    65461 ggtccgaatt tttatggaaa agcatcctga tatccaatta acgatcatgc aacatcattt
    65521 accagatgat attaccgatc ctgtactgta catcacttcg gagatgaagc ctggcatgac
    65581 caatattcct atcatgaaag aacctgttta tgtcgccatt cctaagaaac atcctcttgc
    65641 taaaaaagaa catctgcaat tagaagatat ccaacgcacc cccatcttga tgctgtcaaa
    65701 gagcaacgcc ttccgtcata cactggatgc agcacttgaa gcgaaaagta tcgaattgac
    65761 gatcagttcg acgacagatg atcctgcgac attacgttct attgtcaagc aaggattagc
    65821 tgttagcttt ttcccaaaag tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg
    65881 ggaaatcaaa gatttgccct tgacacgaac aatttattta tcttctagct tcacagaaga
    65941 tcatcctttg atcaaaatga ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa
    66001 aaaaaagagc tgggacaaaa ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag
    66061 aagtaatttc ttagaaaata agccgaaatt tcccaaaaat ttgagaagca attttcgtaa
    66121 attccttctt atttcttgaa gctaaacatt tctgtcacaa cctcttctct attgtcctag
    66181 acattcattg cgttgattta aaacttccat caatcaaatc tgctagttca tctagctttt
    66241 ctattttacg tagatcaata gagatccaat gttctttatt catgtgatag gctggaaaat
    66301 acccctcttt tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaagaatat
    66361 tgatgatcct atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga
    66421 ccccaaacca ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa
    66481 aaatatattc tggagacaaa ttgtatgttt cattgatgtg atcgattagt tcttttttcg
    66541 ttagcatctg cttccatctc tttcttattg attcgcttta ttcaaagata ccacgtaaga
    66601 atagaaaaat gaatacaagt agttgctaat caaatacttc gatatttttc ttttttatca
    66661 actcttaaat gttcaatccg cctgtgttgg tcttgctacc atctactatt aaaatgttta
    66721 ttattgtttg cccacctatt ttttgatttt agtggtatca tttttcaagt tcatgatgaa
    66781 attcttatca tcagtaaatg ctccagaaaa ataaacaatg attgaaaaat cagttgaaac
    66841 ttgaccaccg tttattttct ccgtattctt agaatagaag ggagtctgat tgatgtattc
    66901 actaatgaaa aaaatcatta ccgaaaaaga tattcgacgt catgtttctt tagtagaaca
    66961 attgctgaat catacaaaga taaccgtaaa tgaattagct gaaatcatag ggacaaccga
    67021 acgaacgatt ttttctgacc ttcaatctat tcgctctcag ctgcccgaag gctgggacat
    67081 catcagcgat caagcaggaa tcagtttgca aaaccagcaa aatttattaa caaatgacct
    67141 ttgggagatt tttttcaaac aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac
    67201 aaaaaaagtt gctgtccctg attttctagc cgaccatggg ctttcttatg ggacattgaa
    67261 aagacatgtg actaaaatca atcagcgttt agcaagttac gacttgcaga tcgatttgac
    67321 gaaatataca gcttgtattc ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt
    67381 gctgattcct ttcactcata acaactattt tttcgaggat tattcgatcc atgaatccca
    67441 ttattttcaa tttttaagaa acctcagtca aactgaacta gcggttgaaa cagaagagat
    67501 tttcggaaca tgttggttct ttatcaatac gatccgtata aaagcgaatt gccgattaga
    67561 ttcttctatc cataccaaca gcacactctc ctccctgtat gactctgcat tgaaaaaatt
    67621 gtatttaacg gaaggtattt atcttaaaga cactgagctt tcttttgctt ctttctgctt
    67681 tctggaaagt tggaattaca acaataacta tggacaagaa accgctcgct gcctgcatca
    67741 ttctccattt ttagaggtcc tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct
    67801 tgatcaacta aaaaaaacat ctctgactga taacttggct ctcctcatat taaaatatca
    67861 tgaatcgccg atattgattg aagcattgga ccgtcaatac catcgttttt tagaaacata
    67921 tgatcagcaa tatgcacact tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa
    67981 agaaatagaa atcaatgaac caaattactt tctccaactc ttgtctctac tgatccaaca
    68041 aacgattttt tctatcaaac cgaatttgtt caatgtctat tttattttcc aaggagaacc
    68101 ttcatggaag gcgttcttgc agcaggaatt aaaagattac ttaggaaaac gtgtctgctt
    68161 cttacctatc gaactaaccg atctttccac tatttctttt gaaaaaacag atatcttgat
    68221 ttccaatttc cctctagatt ctctagatat ccctattgta tacatttctt ctattccaac
    68281 gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa acttttttat agtaacggct
    68341 agcgactgta ttctctagct gttttttctt ttttgcgaaa cagtttgttc atgatccctt
    68401 tttctatttc tacaactaag aatagaagaa tcacatttgc tccaatcaat aactgttgtg
    68461 tcatcgttaa acttactgta ccgatcagct gctggccgac tggtagaaaa ataaccatcc
    68521 cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt
    68581 ggttcaatga cggcttggac aactctctgc aattgatcat gtaaacagct tgagctaaga
    68641 cgatattttg cagaagaatg gtttgctgta agctttgccc ttcaaactgc atagccaata
    68701 aataggcagg aaccatgatc agtagggaga catagatgat ccgaaacaaa tgataaccag
    68761 ataggattcc ttgctttgga tctcttggtg ggcgcttcat gatttctgga tctggcgact
    68821 cgaaccctag tgcataagat aaagtgattg tcgttaccat attgacccat aaaatctgta
    68881 ctggggacaa aggtagcggg cgattcagca taagtgccca aatcaagatc aatccttgag
    68941 ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa attatccaaa atccgccggc
    69001 cctcttttac ggcttttgca atcgtatgga aattatcatc tcctaaaacc atatcagctg
    69061 cctgtttgct tacttcacta cctttgattc ccatagcaat tcctacatcg gatcttttca
    69121 aagcaggtgc atcatttacg ccgtctccgg tcattccgac gatttctcca tttttctgca
    69181 aagctttaac aattcgcagt ttatgttctg gtgtcgttcg agcaaagaca tcgactattg
    69241 gcacttgctg aatcaactct tcatcagaca taagatccaa atcgatccct tctaaaactt
    69301 tggcagtatg ttttaagcca atttgttccg caatcgcttt tgctgtgtct ttgtgatccc
    69361 ctgtaatcat tttcactgag atccctgctt gttggcattc ctttacagct ttgactgcac
    69421 tttcttttgg cggatcgatc aatccggcga tccctgcaaa agttaattgg gttaaagtct
    69481 catgcgtgag ttcctgtttc gaatctactt ttttataagc aaatcctaaa acacgctgac
    69541 cttttctcgc aaattctgca gctgtctgac tccatgcttc ttgttctgca gaagaaagag
    69601 ttgatttagc aaataatact tctggtgcac ctttgacgta aatcaccgct cctttttcct
    69661 ccgcatgcat cgttgccata tatttgtatt cagaactgaa cggaatcttt gcttctattt
    69721 ttttcaaagg tagattggct gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc
    69781 cacttaaatt ttctgttttc tgttcttctt ttgtttttaa ttcctgacag tttttcataa
    69841 tggacaacac gcaggcttct tcttttgcag ctacatccac aactgtcatt tcattttttg
    69901 tcaatgttcc tgtcttatct gaacagatca ctgtcataga acctaatgtt tctaccgaag
    69961 gaagtccttt gatgatcgcc ttttcttttg ccatttcatg tactccaacg gatagaatca
    70021 tggtcaaaac agctggcaat ccttcgggta ccattgacac caccaaagca atcgtagccg
    70081 aaagaagaat atgccaatcc ataccatatc gaaatgtcgt aaaaataact aagaataggc
    70141 tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg gttgattttt ttaatcagag
    70201 gcgtcgtttg ttgttcgacc gattgcaatg cttgattgat tttaccgatt tctgtcgtgt
    70261 ttcctgtttc tacaacgaca ccgactgcag aaccagattg tacaagcgtt cctgaaaaac
    70321 ccatattttt ctggtcgccg atcgattctt cttctgtcaa aacggcatga cttttttcag
    70381 atgcttctga ttctcccgtc aaaatcgatt cttccactaa taaatcatgt gcttcgatta
    70441 atcggatatc tgctggaacg acatctccgg cacgtaagac aatgacatct ccttttacca
    70501 gttctttcgc agggattttt tcttgcatcc cgtttgatag tactatcgct tcttgtccca
    70561 tcagctgctt caaaccattc aatgattctt ccgcttttct ttcttgccag taactaacga
    70621 gtccattgac gatcactacg aataaaataa tacttccttc aatatattca ctagaagcaa
    70681 atttcaaaaa agaggcaatg atcaacacga tcatcagcag atcagtaaaa tgctttgcta
    70741 tttttttcca tcgttttgtc tgctgccttg cttttatttc attatatcct tgatcagtca
    70801 ggcgtttctg tcgttcttct tgcgacaagc cttctgttga tgtttgatag tttttcaata
    70861 catcttctac cgtcaatctg taccagttca tcctatcccc tcctgtttaa ttgatactgt
    70921 tattatacgc agggaagttt tttcaggcgt atcgattatt tcaggggggt ctgaaataat
    70981 tcgttttgca aaatgtatct gggcctaact gatatttcta ttgcttattc ttttatatac
    71041 aaaatttaag aatacaaacc agataaccaa taaaaaagga ataaagcgtg tcgtgctttc
    71101 gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac aaagcaagat ctttatatct
    71161 ttgtgtagtg ttccgtttct tttctttaat aaattgaaca tgtgcacaca agatcgaaga
    71221 ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact
    71281 atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat aacattcctg taattaaaat
    71341 accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac caatggggca aacttttttc
    71401 ttttgctgtt tgatataaaa tcctgctagt actgtagact gcactatttg ctgaggacaa
    71461 agaagcaacc aaaatcatta gattgacaat tgctgctccg ccttggcaat ggatcacttg
    71521 cagcatagtc actaaaggac tttcttttaa atctaaacaa tgccatggaa caacaaataa
    71581 aatcgacaaa atcgttccta tatagaaaaa gagaatacgc caaggcagac agttgattgc
    71641 ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg gttaagccga tcatttcaat
    71701 tccagcaaaa ctgaataaaa ccataggaaa tgagactacc attccagcaa agccaaacgg
    71761 tagtaattca tcgatctgtc caaaattttc tttgattccg atagactggg tatgaaaatc
    71821 tgagacactc aatcctatac ccaataagat aaaaaggaaa atccccaaga tcttgatgat
    71881 tgagaaaaaa gattccaatt caccaaacca cttgactgca acaaggttga ctgccattaa
    71941 gatcattagt gcgctacctc cgcttatcaa agctggtaca tgtacaaacc agaaatggat
    72001 atacaatcct attgctgtta attccgcaag tccagctcca agccagcaaa gccaataagt
    72061 ccaattgaca aaaaatgccc actttttgcc taaataatag tcaacgaaat cactgataga
    72121 gttaaatgta ctatttgcta aaaataaatc acccatggcc cgcatcaaac cataaatagc
    72181 caaaccgcat atcaaataaa cgagaataac agctggccct gctgatgcaa ttgcctttgc
    72241 tgaacctaaa aataatcctg ttccaattgc gccacctaat gcgattaact ggatatttcg
    72301 attagttaga gtccgtttca ttgccggttt ttccatcaag ttcacctttt tctttttcaa
    72361 gcagcatggc gacttcctgt tcccaagaag gtttctttat tttttcaata cagagtggta
    72421 ctacaaacaa aatgacactg atggctgtga ctgcaattag attgatccat gttccagctt
    72481 tcatcgtcag tccgacagaa aatccgattg ccgcaaataa aacaataacg acagtccaaa
    72541 ctccaagatt gctctttttc tctttttttc caattctaaa tggtcgattc aactttgcat
    72601 gtgtataacg caatctcaaa actgcgatcc ccatgatgac atagacgata cagtagataa
    72661 cagtagttga atttacgagc atcaaaaatg cttgattgac gccaggaatc agcaaataaa
    72721 aaagtgcaaa gattgagata atgacagact gagtcaaaat aacggatttg gatacgtcaa
    72781 attgattcgt cttccaaaaa ttgaattttg gtggatacgc accttttcta gcactttctg
    72841 tcactgtctt tgctggtcct gaggcccaag cagataattg gactgctaca ccaatgaaga
    72901 caagtaaact aaacagattc acaataagat gtggcaaacc aagaatttgg caataaatcg
    72961 aaatagattg cgcgatatta ttcaattcca tttgtccttt tggtacgaca tttgctacaa
    73021 ctaaagcatt caaaatattg acgaaaaata taaaaattaa cgcggcaaat actccgcgaa
    73081 tatacgtttt aactggattt tccaatcgag tgatatacac agaggacatt tcgataccag
    73141 taaaaataaa catgatcggc gcaaaataaa caaatgagga tgcagtcgta gtatctggta
    73201 ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg tacgatccca actttgatcc
    73261 acgcagcaaa ccctaataag agcatcatcg tcaaaggcac gtacaatcct aaccaaacac
    73321 cgacttttcc gccgattttt gccatatcaa atttcaaatt caaaaaagtg atgatccaat
    73381 aaacgactaa aatgacaact aatgtaaatt tgctgttcaa accaagcggc acattatcga
    73441 taatattacc aaacaacgga gcgagtgctg aagcgaccat caccatccca ggaaacattt
    73501 ggacccataa cagccatgag acaacgaacc cccatttttt tcctagagca ttcgataccc
    73561 aaagttctgg accacctttt tggggaaaca ttccagcaaa ttcaccagag atcaatgaga
    73621 ttggaaaagc gtacaataat actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg
    73681 ctgctacatc tggaatattt cgtacacttg ctacaagggc gcaggtcatt ccgataaagg
    73741 tcatggtcgt gatttgcttt gatgaaccag tatctttcat ctttattcct tctttcaata
    73801 gcgacatatg tttttgtttt cttttatttt tgttcaagaa cagaaatgac aaggtttcat
    73861 cgattcagta caaaacccag tatcaccgaa acatccattg aacgatctta gtgaactggg
    73921 tttgatacat gaaacgaacg atccaactta tagaacgttt tataaaaaag aatatcttgg
    73981 cgaggaatat atgaaaaaca gggggaaata caattgtttt gatgaagcaa ataattgtct
    74041 gttctccata tgtctcttgt tattctttca cacaaaaatc agttctattg gatgatttcg
    74101 tttagtttct tttccattgc tttggcgatt ttttttgtat aaaaattgaa aattcgatca
    74161 tcattcaaat aaggagtcat gatagccgca cgtagcagag tgattttctg ttcttttttc
    74221 cattcttctt tcgttagacc cattctttca acaaaacgaa tcggagaatc accgtaatct
    74281 tcttgtgtaa atgtagtatg agaagtaata aaccgttcac cataaacatc gccatccata
    74341 taagaagacc gatcgaacat tttttcattt aattcattga tagcgttcaa atctgtacag
    74401 ccttgttctt tgaataccca attgaccata ttaaaatcag gatgattcaa tggatagact
    74461 tcgattgttt ttcctttaac agtaaaagtc agatgatcga gaaattctct gaatctttga
    74521 gctgcctcga ttgaagcccc aatcaattga ccatatccgg tcacattcaa tggcaataca
    74581 cgatgtgctg tccagactgc ggcggcggtt gctccagctt ttgatccttc caaaatataa
    74641 gctcctaaca tatctggagc ctttactgat ttttcaaaca catatggcgc gaaataagaa
    74701 atgatatttc tcatattttt atgtttgatt acgattcctc cagcagcata agggacatag
    74761 cccatcttat gaggatctat cgtgacagaa tccgcttctg aaatagctct gaacccttca
    74821 tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat gatgttcttc aaagaattca
    74881 gctaaggaag catacggcac aaattctcct gcttcgttta gaaacagtga acgcgcatag
    74941 ccgccatatg cggcatcaac atgtaaatag aaatagatcc cttcatcttt taacctttct
    75001 cttaattgga cgattttatc tacactgtct acttgacctt cttctgtcgt tcctacgaca
    75061 gcaaccacac ctagaattgg gattttttgt ccagctaatt cacgaattgt tttttccaaa
    75121 gcattaatat ccattcgata atcctcttga actgggatag caaccatctg atctaagccg
    75181 actccgcaaa tatctaacgc cttcatccaa gaatagtgtt ttgtttgagg gaccagccat
    75241 ttccctaatc tttggatatt ttttccgcta cgggaagaag ctgctttgac ctcatccatt
    75301 tcttcgtccg taaagctttc ggtcatttcc aaaatttctt caacagataa attcaaaagt
    75361 tcccactcag acattttttt cactttttca ggcagcactt ccttgacagc taaaggaatc
    75421 gatttgatgc aacgggcgta ccataatcct tcaagattag caatagaacc atctgctgca
    75481 atgtgtcccc atccgtctgt catattgaat aatgaagcaa aatcctgtcc tacttctgct
    75541 tccatttgcg aagttgccat agaagattcc aatgcaacat tatttgggtt ccacagcatc
    75601 gcatagttgt aagctaataa agcaggcatc aatgtttcag cattcatttg tccccagtaa
    75661 cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc gctgactgac ttcatccaag
    75721 acctctatca tatggtcgcg ggtggctaga taatctggag tgagcttatc tccttcgcta
    75781 attgcaggca tatctgaagg aatataattt tctctccatc caagatgttc atctaccatc
    75841 ttgttcaaca acattttata aactggtcca ttttcagctt tgtcgcctat aaagacggcc
    75901 ttgatatcca tatctttcat ttttcttccc tccatatttt gttggtcaat gtcttgcaga
    75961 tacatgtgtt tatctgtaac acctcttcac tatagcgcca aaaatgagaa tttttttttt
    76021 ataacatcaa atccacgtag atttttacaa acaaaaataa ataaagtcgg atttttctga
    76081 taattcaacg caaaaaagcg aacaattttt caataaatca gtcttctaaa atagataacc
    76141 taaaaaaagc gaacaaacaa ataaaatga
//
LOCUS       DASJRQ010000003        62016 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJRQ010000003 DASJRQ010000000
VERSION     DASJRQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.003"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            204..722
                     /locus_tag="U4A80_000140"
     CDS             204..722
                     /locus_tag="U4A80_000140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1973814.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            850..1533
                     /locus_tag="U4A80_000141"
     CDS             850..1533
                     /locus_tag="U4A80_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1973815.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1842..1915
                     /locus_tag="U4A80_000142"
     tRNA            1842..1915
                     /locus_tag="U4A80_000142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1876..1878,aa:Arg,seq:tct)
     gene            2074..2433
                     /locus_tag="U4A80_000143"
     CDS             2074..2433
                     /locus_tag="U4A80_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1973816.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2556..4187
                     /locus_tag="U4A80_000144"
     CDS             2556..4187
                     /locus_tag="U4A80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1973817.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4249..4890)
                     /locus_tag="U4A80_000145"
     CDS             complement(4249..4890)
                     /locus_tag="U4A80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1973818.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5076..5152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5172..5480
                     /gene="rplU"
                     /locus_tag="U4A80_000146"
     CDS             5172..5480
                     /gene="rplU"
                     /locus_tag="U4A80_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1973819.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5507..5851
                     /locus_tag="U4A80_000147"
     CDS             5507..5851
                     /locus_tag="U4A80_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1973820.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5864..6157
                     /gene="rpmA"
                     /locus_tag="U4A80_000148"
     CDS             5864..6157
                     /gene="rpmA"
                     /locus_tag="U4A80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1973821.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6591..9398
                     /locus_tag="U4A80_000149"
     CDS             6591..9398
                     /locus_tag="U4A80_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1973822.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9590..10081
                     /locus_tag="U4A80_000150"
     CDS             9590..10081
                     /locus_tag="U4A80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1973823.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10184..11173
                     /gene="manX"
                     /locus_tag="U4A80_000151"
     CDS             10184..11173
                     /gene="manX"
                     /locus_tag="U4A80_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1973824.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11206..12009
                     /locus_tag="U4A80_000152"
     CDS             11206..12009
                     /locus_tag="U4A80_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1973825.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12030..12944
                     /locus_tag="U4A80_000153"
     CDS             12030..12944
                     /locus_tag="U4A80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1973826.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13027..13401
                     /locus_tag="U4A80_000154"
     CDS             13027..13401
                     /locus_tag="U4A80_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1973827.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13540..13785
                     /locus_tag="U4A80_000155"
     CDS             13540..13785
                     /locus_tag="U4A80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1973828.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13957..15021
                     /locus_tag="U4A80_000156"
     CDS             13957..15021
                     /locus_tag="U4A80_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1973829.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15267..15692
                     /locus_tag="U4A80_000157"
     CDS             15267..15692
                     /locus_tag="U4A80_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1973830.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15685..16134
                     /gene="nusB"
                     /locus_tag="U4A80_000158"
     CDS             15685..16134
                     /gene="nusB"
                     /locus_tag="U4A80_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1973831.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16231..17085
                     /locus_tag="U4A80_000159"
     CDS             16231..17085
                     /locus_tag="U4A80_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1973832.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17086..18423
                     /locus_tag="U4A80_000160"
     CDS             17086..18423
                     /locus_tag="U4A80_000160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1973833.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18428..18652
                     /locus_tag="U4A80_000161"
     CDS             18428..18652
                     /locus_tag="U4A80_000161"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1973834.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18654..19544
                     /locus_tag="U4A80_000162"
     CDS             18654..19544
                     /locus_tag="U4A80_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1973835.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19553..20371
                     /locus_tag="U4A80_000163"
     CDS             19553..20371
                     /locus_tag="U4A80_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1973836.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20478..20927
                     /locus_tag="U4A80_000164"
     CDS             20478..20927
                     /locus_tag="U4A80_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1973837.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20945..22627
                     /gene="recN"
                     /locus_tag="U4A80_000165"
     CDS             20945..22627
                     /gene="recN"
                     /locus_tag="U4A80_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1973838.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22682..22798)
                     /locus_tag="U4A80_000166"
     CDS             complement(22682..22798)
                     /locus_tag="U4A80_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1973839.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22871..23827)
                     /locus_tag="U4A80_000167"
     CDS             complement(22871..23827)
                     /locus_tag="U4A80_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1973840.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23998..24366
                     /locus_tag="U4A80_000168"
     CDS             23998..24366
                     /locus_tag="U4A80_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1973841.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24536..24967
                     /gene="mraZ"
                     /locus_tag="U4A80_000169"
     CDS             24536..24967
                     /gene="mraZ"
                     /locus_tag="U4A80_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1973842.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24988..25947
                     /gene="rsmH"
                     /locus_tag="U4A80_000170"
     CDS             24988..25947
                     /gene="rsmH"
                     /locus_tag="U4A80_000170"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1973843.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25952..26353
                     /gene="ftsL"
                     /locus_tag="U4A80_000171"
     CDS             25952..26353
                     /gene="ftsL"
                     /locus_tag="U4A80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1973844.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26354..28546
                     /locus_tag="U4A80_000172"
     CDS             26354..28546
                     /locus_tag="U4A80_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1973845.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28572..29534
                     /locus_tag="U4A80_000173"
     CDS             28572..29534
                     /locus_tag="U4A80_000173"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1973846.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29548..30909
                     /locus_tag="U4A80_000174"
     CDS             29548..30909
                     /locus_tag="U4A80_000174"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1973847.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30935..32023
                     /gene="murG"
                     /locus_tag="U4A80_000175"
     CDS             30935..32023
                     /gene="murG"
                     /locus_tag="U4A80_000175"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1973848.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32038..33258
                     /locus_tag="U4A80_000176"
     CDS             32038..33258
                     /locus_tag="U4A80_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1973849.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33414..34739
                     /gene="ftsA"
                     /locus_tag="U4A80_000177"
     CDS             33414..34739
                     /gene="ftsA"
                     /locus_tag="U4A80_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1973850.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34762..36003
                     /gene="ftsZ"
                     /locus_tag="U4A80_000178"
     CDS             34762..36003
                     /gene="ftsZ"
                     /locus_tag="U4A80_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1973851.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36020..36697
                     /locus_tag="U4A80_000179"
     CDS             36020..36697
                     /locus_tag="U4A80_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1973852.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36711..37310
                     /locus_tag="U4A80_000180"
     CDS             36711..37310
                     /locus_tag="U4A80_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1973853.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37325..37612
                     /locus_tag="U4A80_000181"
     CDS             37325..37612
                     /locus_tag="U4A80_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1973854.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37661..38443
                     /locus_tag="U4A80_000182"
     CDS             37661..38443
                     /locus_tag="U4A80_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1973855.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38464..39171
                     /locus_tag="U4A80_000183"
     CDS             38464..39171
                     /locus_tag="U4A80_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1973856.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39433..42231
                     /gene="ileS"
                     /locus_tag="U4A80_000184"
     CDS             39433..42231
                     /gene="ileS"
                     /locus_tag="U4A80_000184"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1973857.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42280..43806)
                     /locus_tag="U4A80_000185"
     CDS             complement(42280..43806)
                     /locus_tag="U4A80_000185"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1973858.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43821..44468)
                     /locus_tag="U4A80_000186"
     CDS             complement(43821..44468)
                     /locus_tag="U4A80_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1973859.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44652..44981)
                     /locus_tag="U4A80_000187"
     CDS             complement(44652..44981)
                     /locus_tag="U4A80_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1973860.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45158..45886
                     /gene="sfsA"
                     /locus_tag="U4A80_000188"
     CDS             45158..45886
                     /gene="sfsA"
                     /locus_tag="U4A80_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1973861.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45902..46915
                     /locus_tag="U4A80_000189"
     CDS             45902..46915
                     /locus_tag="U4A80_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1973862.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46915..48192
                     /locus_tag="U4A80_000190"
     CDS             46915..48192
                     /locus_tag="U4A80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1973863.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48255..50957
                     /locus_tag="U4A80_000191"
     CDS             48255..50957
                     /locus_tag="U4A80_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1973864.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51109..51426
                     /locus_tag="U4A80_000192"
     CDS             51109..51426
                     /locus_tag="U4A80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1973865.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51456..51776
                     /locus_tag="U4A80_000193"
     CDS             51456..51776
                     /locus_tag="U4A80_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1973866.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51884..53344
                     /locus_tag="U4A80_000194"
     CDS             51884..53344
                     /locus_tag="U4A80_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1973867.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53412..53633
                     /locus_tag="U4A80_000195"
     CDS             53412..53633
                     /locus_tag="U4A80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973868.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53664..53846
                     /locus_tag="U4A80_000196"
     CDS             53664..53846
                     /locus_tag="U4A80_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1973869.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53846..54259
                     /locus_tag="U4A80_000197"
     CDS             53846..54259
                     /locus_tag="U4A80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1973870.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54382..55563
                     /locus_tag="U4A80_000198"
     CDS             54382..55563
                     /locus_tag="U4A80_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1973871.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55634..55798)
                     /locus_tag="U4A80_000199"
     CDS             complement(55634..55798)
                     /locus_tag="U4A80_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973872.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55952..56025
                     /locus_tag="U4A80_000200"
     tRNA            55952..56025
                     /locus_tag="U4A80_000200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55986..55988,aa:Arg,seq:cct)
     gene            complement(56094..57233)
                     /locus_tag="U4A80_000201"
     CDS             complement(56094..57233)
                     /locus_tag="U4A80_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1973873.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57532..58167)
                     /locus_tag="U4A80_000202"
     CDS             complement(57532..58167)
                     /locus_tag="U4A80_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973874.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58280..58915)
                     /locus_tag="U4A80_000203"
     CDS             complement(58280..58915)
                     /locus_tag="U4A80_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN1973875.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58949..59410)
                     /locus_tag="U4A80_000204"
     CDS             complement(58949..59410)
                     /locus_tag="U4A80_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN1973876.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59540..59971)
                     /locus_tag="U4A80_000205"
     CDS             complement(59540..59971)
                     /locus_tag="U4A80_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1973877.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59989..60309)
                     /locus_tag="U4A80_000206"
     CDS             complement(59989..60309)
                     /locus_tag="U4A80_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1973878.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60608..61384
                     /locus_tag="U4A80_000207"
     CDS             60608..61384
                     /locus_tag="U4A80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1973879.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61399..61602
                     /locus_tag="U4A80_000208"
     CDS             61399..61602
                     /locus_tag="U4A80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1973880.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61618..61956
                     /locus_tag="U4A80_000209"
     CDS             61618..61956
                     /locus_tag="U4A80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN1973881.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        20459 a        10427 c        13009 g        18121 t
ORIGIN      
        1 ttcctttcta aaacttgtaa tgttagggta aacgtttttt aaactaacgt caattattta
       61 taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt attaaaggga
      121 aacattttta cagctataat ttgaaaaaat ttatcgttat tatatgatta agaaggattg
      181 aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta
      241 taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg
      301 gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc
      361 tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt
      421 atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg
      481 aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga
      541 gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa
      601 cggcatttgc atggattcag gggttattct ttacgattgc tttttgccta acaggaatca
      661 cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt
      721 aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca
      781 cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg
      841 aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat
      901 tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct
      961 catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt
     1021 ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt
     1081 gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc
     1141 atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg
     1201 cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt
     1261 atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga
     1321 aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat
     1381 gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt
     1441 gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta
     1501 atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa
     1561 tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa
     1621 ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt
     1681 ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag
     1741 tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag
     1801 caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg
     1861 atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa
     1921 aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt
     1981 gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa
     2041 aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg
     2101 actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct
     2161 gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa
     2221 acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga
     2281 gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt
     2341 gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta
     2401 gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga atttatctaa
     2461 caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt
     2521 attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac
     2581 taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga
     2641 tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg
     2701 atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag
     2761 gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg
     2821 cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac
     2881 ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta
     2941 cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg
     3001 gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat
     3061 ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct
     3121 atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag
     3181 cagttatgct attgttccat ttaccagtag caactgtggg taagattcca caaacattga
     3241 tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag
     3301 ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg
     3361 ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg
     3421 gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa
     3481 gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct
     3541 tactgctctc catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg
     3601 caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat
     3661 ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta
     3721 tctttgattt aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg
     3781 tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc
     3841 cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta
     3901 tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt
     3961 tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc
     4021 aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag
     4081 aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc
     4141 atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag
     4201 aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg
     4261 taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta
     4321 gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta
     4381 ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt
     4441 ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat
     4501 taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc
     4561 ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat
     4621 ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg
     4681 tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg
     4741 aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg
     4801 aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc
     4861 ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag
     4921 aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga
     4981 agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct
     5041 gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa
     5101 gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg
     5161 tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg
     5221 gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg
     5281 aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa
     5341 ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca
     5401 aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta
     5461 tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac
     5521 atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc
     5581 tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt
     5641 caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg
     5701 cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga
     5761 gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata
     5821 tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa
     5881 tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga
     5941 atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct
     6001 ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga
     6061 cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa
     6121 acaagtgtct gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt
     6181 tgtgacaaga cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc
     6241 tacgaagttt actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg
     6301 ttttgataac tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat
     6361 tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc
     6421 gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta
     6481 aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag
     6541 tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca
     6601 aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa
     6661 ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc
     6721 gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat caaacactcg
     6781 ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg
     6841 aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta
     6901 acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag
     6961 gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga
     7021 tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca
     7081 attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa
     7141 aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg
     7201 gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt
     7261 actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa
     7321 atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg
     7381 ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga
     7441 aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt
     7501 ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac
     7561 aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac
     7621 ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat
     7681 tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg
     7741 tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc
     7801 tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag
     7861 caatcaatca tttcatttct acagatatca acatccatct gaagtctttc tataaagacc
     7921 atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag
     7981 aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa
     8041 actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag
     8101 aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg
     8161 ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa
     8221 gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca
     8281 gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg
     8341 tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc
     8401 cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg
     8461 tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca
     8521 ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac
     8581 gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc
     8641 gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga
     8701 aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat
     8761 tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga
     8821 ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta
     8881 ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc
     8941 aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat
     9001 tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact
     9061 tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct
     9121 ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca
     9181 cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat
     9241 tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg
     9301 aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc
     9361 ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt
     9421 tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta
     9481 aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca
     9541 gaaatgatag gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg
     9601 attggcacgt atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca
     9661 aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa
     9721 gtttctacta aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa
     9781 aatgattcaa atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac
     9841 caatccccaa gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat
     9901 cggcggtatg agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca
     9961 acaagatatt gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa
    10021 agtacctgct gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta
    10081 gcaagaatgg ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat
    10141 atttttgaat ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct
    10201 agcaagtcat ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga
    10261 acaagaaaac gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc
    10321 caaattggaa gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga
    10381 tttatggggc ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa
    10441 atgggcaatc gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt
    10501 ttcaatggaa tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt
    10561 aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc
    10621 acaacctaaa ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt
    10681 attagcacgt gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc
    10741 agtgcaacca aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa
    10801 gagactaatc gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa
    10861 aatgatcgaa gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga
    10921 aaatcctgaa gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt
    10981 tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca
    11041 agaagacgtt gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa
    11101 agtacctaat gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt
    11161 agcaaaagcg taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca
    11221 ataattttag tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt
    11281 caatttcatc aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa
    11341 gctggtatcg tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt
    11401 gccgctgtag caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta
    11461 ggtggacaag gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt
    11521 gcagggttgt tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg
    11581 gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta
    11641 tgtttacaag gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa
    11701 actgtgcaat cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt
    11761 gggggaatgg tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa
    11821 gtatggccat tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt
    11881 gctctaggtg ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt
    11941 ggttcatcaa ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat
    12001 gactattaat cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa
    12061 aagatcgttt agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac
    12121 gtatgcaaaa cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa
    12181 caaaagaaga acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat
    12241 acattgcttc tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg
    12301 cgcctgttga tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg
    12361 gtgttggtga cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt
    12421 cattagctat gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta
    12481 ttcgttgggc attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta
    12541 ctgatgacct ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga
    12601 tgttcgtatt agctgccctt gtacaaagat gggtatcaat caaattctta ccagttgtat
    12661 caactgtaaa attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg
    12721 aaggaatcaa gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg
    12781 taacaacatt acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa
    12841 cattgttatg tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt
    12901 tattcgtagt cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc
    12961 tcgggcgttg tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg
    13021 gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt
    13081 acgggtctga cagattacgg gcaaatcatg ataggtaata atggttttga gttttaccat
    13141 gcgcgagata gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca
    13201 tcagtcatgc taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt
    13261 acatacacat tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta
    13321 gatgaagata gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt
    13381 agtatgttta aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt
    13441 ttttactttg cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc
    13501 catatgctaa aattgattag caaagaagga ggttatatta tgagttggtt atggacattg
    13561 attgttggag ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg
    13621 ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc
    13681 ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg
    13741 attttggtag cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag
    13801 taaactaaaa ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata
    13861 aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa
    13921 tggaaaaaag cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg
    13981 cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt
    14041 cgttacttaa cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca
    14101 ttttttatta ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa
    14161 attattaaaa atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa
    14221 ctgcactcat tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa
    14281 gaaatcatcg atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg
    14341 aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat
    14401 gatcacatcc tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta
    14461 gacttttata tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc
    14521 ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac
    14581 ttgatcacat tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca
    14641 ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct
    14701 caattagcag ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt
    14761 gctagagatt atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat
    14821 gggatcggtt tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt
    14881 gtaccaggaa atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta
    14941 cgtattgagg atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca
    15001 aaagaattga tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc
    15061 acagactttg ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc
    15121 tgtaagagat aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc
    15181 ggcagttttc ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt
    15241 gcttaggcaa agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa
    15301 accaagattt aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg
    15361 cagcttctaa agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg
    15421 aattattagg acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct
    15481 tgaaagtaga tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg
    15541 aaatgcagga acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag
    15601 tcaatatcca tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac
    15661 tttttgaaga cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa
    15721 atggcattgc aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg
    15781 attttcaacg caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt
    15841 gttcctgttt atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat
    15901 aaagtgattg aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat
    15961 ttggtgatct tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct
    16021 gttgctttaa atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa
    16081 ttcgtcaacg gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta
    16141 gcttttttct atgaatcatc ataaacctgt tataaatcgg gaaaattcta tggtagaatg
    16201 aaaacacaaa aaatatctgg gagtggttag atggcagaat tgattaacgg aaaagaatta
    16261 gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga taatggtatc
    16321 catcccggat tagtcgtttt attggtaggg gaaaatccag ccagccaaat ctatgtaaga
    16381 aataaagaac gtgtagcaaa agaaatcgga atccattcat tggtagagcg ttatcctgag
    16441 acgatttctg aagacgaact acttgcagaa attgaaaaat acaatcaaga tcctagtttt
    16501 catggtatat tggtccaatt accattgcca aagcatatag atgaagaaaa gatcctgtta
    16561 gctatccgcc ctgaaaaaga cgtagacggt tttcatccga tgaatttagg cagactcctt
    16621 gcgggtaaac cggacaagat tccttgtact ccatacggca tcatgaaaat gtttgaagct
    16681 tattctatcg atccggcggg taaacgagca gtagttatcg gacgaagcaa tatcgtcggt
    16741 aaaccgatgg ctcagctact cttgatggca gatgcaactg ttactgttgc tcattctaaa
    16801 accgaaaatc tggctgaact ggcacaagaa gcagatattt tagtcgtagc aataggacgt
    16861 ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga tgtagggatg
    16921 aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt ggagcctatt
    16981 gcaggctgga tcactcctgt gccaaagggt gtagggccaa tgactatcac gatgctgatg
    17041 tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg aatagatggc acaagaatat
    17101 ttaactgtca ctacattaac gaagtatttg aaaagaaaat ttgatgccga tccttattta
    17161 gaacgcgtat atttaacagg cgaaatatcg aatttccgtt tacgtgccaa tgcccatcaa
    17221 tatttcagct taaaagatga ccatgcaaaa atatctgcta ttatgttcaa aggagcattc
    17281 caaaaattac gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg gcgtatttca
    17341 ttatatgaag cgggaggttc ttatcaaatc tatgtagagc acatggagcc tgatggaata
    17401 ggtgctttat atcaagaatt ggaagaacga aaagaaaagc tgatgaagga aggccttttt
    17461 caagggccta aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct taccagtcca
    17521 agcggagcgg ttatccgcga tatcatcacg acggtgaaaa gaagatatcc aattgtccaa
    17581 ttggttttgt tcccaacagt tgtacaagga aaccaagcag cggacaatgt tgtcaggaat
    17641 atccagagag tagaagagat gggcaatttc gatacaatga tcatcggtcg tgggggtggt
    17701 tcgattgagg atttatggcc gtttaatgaa gagcgggttg ctcgggcgat ttatcaagct
    17761 aatacgccgg tgatttcatc tgtaggacat gaaacagata ccacgatcgc tgatttagta
    17821 gcagatgttc gggctgccac tccaacagca gctgcagaat tagctgttcc tgttttgtca
    17881 gaagagttga tgcgcattca agaacggcaa gctagattgc agcaggcgtt tgtacgccag
    17941 atccagcgca aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt tcggcagcct
    18001 gaaagattgt atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg attatatcaa
    18061 gctacacagc aaatttatca tgcgaaagaa aaacaaacga tcgcattaag ccaccaattg
    18121 gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc ttatctagaa
    18181 aaacgcttag aagagaaaat ccaacaatat atgaaacaac agcagcagcg ttttgctcaa
    18241 gctgttcaat ctcttgattt gttgagtcca ttgaaaatca tgggaagagg ctacagctat
    18301 acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg ggatttgctg
    18361 acgatccatt atcaagatgg aatcgttaaa ggacaagtac aagagataga caaaggagaa
    18421 taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa aaattgtcat
    18481 gcagttagaa caaggagacg ttccattaga atcagctcta gattcgttta aacgaggaat
    18541 ggaattaagc aaacattgcc aagatacatt agctaaagca gaagagacct tgacgaaaat
    18601 gatgacagaa tcaaatgaag aagttgattt tgacggaaac gaggaaagct gagatgagat
    18661 taactgactt ttcagctatt catctacctg ctgtagaaaa agaaatcttg tcttttttag
    18721 atgagcatac aacggataga ggactctatg atgcgatgag ttattcagtg aaagcaggcg
    18781 ggaaaagaat acgtccctta ttacttttaa cggccgttgc ttcctttgat gagcctattg
    18841 atgttcccgt ctatcaagta gcagctgctt tagaaatggt gcatacgtat tccttgatcc
    18901 acgatgattt gcctgcgatg gacaatgatg atcttcgtag aggaaaacct accaatcata
    18961 aagtattcgg cgaagcatta gctatattgg ccggagatgg tttgcttaca ggagcattcc
    19021 agctaatcag tatggcacac ttaggtaatt ctcctaagct tctgctgctt cagcaattag
    19081 ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc tgcagatatc gagggcgaat
    19141 cgaaaaagct ttctttagaa gaattagcat ttattcatga aagaaagaca gggcatttga
    19201 ttcgttatgc cttgctggct ggtggcatat tagcgaagca acctgaagaa atcttattgc
    19261 tacttcaacg attagccgaa catttaggtt tagcttttca gataagagat gatctactcg
    19321 atgtgattgg tacaacgaaa acattaggga aaacagctgg taaagatgaa cgtatggaaa
    19381 aaaatactta cccgcgttta ttaggcctag aaaagacacg tgaggcgttg gagattgagc
    19441 tgttatccgc caacaagatc atagacaaat tagaagagga cgttttttct tttgatggcg
    19501 ggctgatgcg ccagatgatc aagcaatttg atgtggagaa atgagggaaa gaatggaaaa
    19561 agaacgcgta gatgtattag cggtcaaaca aggattattt gaaacaagag aacaagccaa
    19621 acgttcagtg atggctggtt tgatttacaa cgaaaaaaac gagcggtttg ataaaccggg
    19681 agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc cttatgtttc
    19741 tcgaggggga ttaaaattag aaaaagcact caaagcattt gatctatctg ttgaaggaaa
    19801 aacattgtta gatattggtg catccactgg cggatttact gatgcagctt tgcaaaatgg
    19861 cgcaaaaatg agttatgctt tagacgttgg ctacaatcag ctagcttgga aaataagaca
    19921 agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac ccgaagactt
    19981 taccttagga attccagaag ttgctgttat cgatgtttct tttatttcac taaaactgat
    20041 gctgcctcca ttacatgcta ttttaaaaga agagggagaa gtgattgctt taatcaaacc
    20101 ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga attgttcgtg accctcagac
    20161 ccatcaaaaa gttttagaag acattttgtc atttgcggca caaggtggat atgatgtgct
    20221 ggaattaagt tattctccga tcacgggggg agaaggaaat atcgagttct tggctcactt
    20281 gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt aatatggcag aagtggtttc
    20341 aaatgcacac gaacagtttg accataaata aattgtaact gtgaaaatca agtgagaagt
    20401 atgcggattc ttctttatta taccaaggaa ataagctatc attgattaag aagaatatgc
    20461 taaggagcga agacgtaatg agaaaaaaag atcggcatcg tttgattact cgattgttga
    20521 gtgagcaaga tatccgaaag caggaagaat ttgttgaaat cctgaaaagc aaaggaatat
    20581 ctgttactca agctacgatt tccagagata taaaggaact gaaactgatc aaggtaccgg
    20641 cttcagatgg cggctaccgt tatagcatac cagctgaaac aaacgaagat gtaaatacta
    20701 aattggaaaa actattaaaa gacgctttcg tggcagttga ccaaatggaa aaatttgtta
    20761 ttttgaaaac attgccggga aatgcttcgg cagcagcaaa tttgattgat aaacgttaca
    20821 agaaagaatt attttcgatt atcaatgatg acgataatgt tttgatgatt acacgaactg
    20881 aagaccaggc agttttattg aagaaagact tcctgcatta cctttaagtt gaggagcgaa
    20941 tgtgatgcta caagaaatca gtattactaa ctttgcgatt attccggaat tgcggctttc
    21001 ttttcacgaa ggaatgaccg cgctgaccgg agagactggt gcgggtaaat ccattattat
    21061 cgatgcttta ggtctgttag caggaggaag aggttcaagc gattatatac gtcaaggagc
    21121 agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa caagaaggat tctctgaact
    21181 gatggttgaa ttagggatcg aaacagatga ggacaatctg attgttcgac gggatatgtc
    21241 cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt atcacattag ctaatctgag
    21301 aagaatcggc agttatttag tagatatcca aggacaaaac gaacatcagg aactgctcca
    21361 gcctgaatca catcttgctt tattggatcg ttttggagat gtagctttcc aacagaagaa
    21421 aaagagttat cagcaagaat atatctctta tcgtgagctg gaaaaaaggg tacgaaaagt
    21481 gcagcaaaat gaaaaatcct atgttcagag aatagatatg ctccatttcc agcaagaaga
    21541 aatagctgct gctgatttgc aagtcggaga agaagagaaa ttaaaagaag aaagagaaaa
    21601 acttagcaat tatcaaaaaa ttgctgatgg attagctgca ggttacggag cactgacgaa
    21661 taatgaacaa aacagtgtag atggtgttgg cttggccgtt tcagaactgc aagggatcgc
    21721 ccatctagat gtggagtatg aagcaattta tgaaaatatc caaagtgctt actatttgct
    21781 gcaagatgca atcggcgata tgagtcgtca gattgattta ttggagttag atgagagtcg
    21841 tttagaagaa gttacgcaac gtttagaatt aatccgacag ttgaaacgta aatacggcga
    21901 atccattgag tcaatattgg cttactatga cgaaatcacc gaagagctag cttcttctga
    21961 tttttcagaa ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag agcttctact
    22021 tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag aattggaaaa
    22081 atccattctg catgaattga aaagtctgta tatggaaaac actgagtttg aggtacgttt
    22141 cttaaaagaa gaaaatagac aattgaatca tgatggattt gaccaaatcg aattttacat
    22201 tacgaccaat ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg gtggcgaact
    22261 gtctcggatg ttgcttgctt tgaaaactat tttttcttct gagcaaggtg tgacaagtat
    22321 tatttttgat gaagtggata caggtgtgag cggaagagtt gcacaggcca tcgcagataa
    22381 aatttctcaa atatcgaaat actctcaagt tttgtgcatc actcatctgc ctcaagttgc
    22441 tgcagtcgcc gactatcaat attatattgt gaaagcagtg attgatggac gtacgcaaac
    22501 ttctgtttca gagttgaaaa caaaagaaag agaagaagaa attgcacgta tgctggctgg
    22561 aagtgagatt acaaaactca cactcgaaca tgcaaaagaa ctattgaaac tcgcaaaaag
    22621 gacctgagcc acctcgcagt ggttcaggtc cttttcagca gctgatagct atattttatt
    22681 tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg
    22741 tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg
    22801 ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa
    22861 gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa
    22921 ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa
    22981 tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag
    23041 ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca
    23101 tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca
    23161 atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta
    23221 gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt
    23281 ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca
    23341 aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc
    23401 aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc
    23461 taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg
    23521 gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt
    23581 tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt
    23641 cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt
    23701 caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga
    23761 tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt
    23821 tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat
    23881 acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca
    23941 gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg
    24001 ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt
    24061 tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat
    24121 ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt
    24181 tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc
    24241 caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg
    24301 gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac
    24361 ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt
    24421 ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga
    24481 aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt
    24541 gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact
    24601 tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg
    24661 ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa
    24721 aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga
    24781 taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg
    24841 ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga
    24901 tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg
    24961 actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg
    25021 ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca
    25081 ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg
    25141 tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat
    25201 gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg
    25261 agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc ctctcctcag
    25321 ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg
    25381 gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta
    25441 gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc
    25501 gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa
    25561 caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa
    25621 gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca
    25681 atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac
    25741 agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt
    25801 cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt
    25861 ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca
    25921 gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta
    25981 cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc
    26041 gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt
    26101 ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt
    26161 tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat
    26221 caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac
    26281 tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag
    26341 gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct
    26401 aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt
    26461 gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac
    26521 ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag
    26581 aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat
    26641 taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa
    26701 gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc
    26761 actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta
    26821 tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg
    26881 tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc
    26941 ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact
    27001 aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca
    27061 aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga
    27121 cggacaagat atctacacga ctttagacag cagactacaa agttatttag aaacactgat
    27181 ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa
    27241 aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg
    27301 attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg
    27361 atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa
    27421 tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt
    27481 tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat
    27541 ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt
    27601 tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa
    27661 tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat
    27721 gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag
    27781 caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca
    27841 acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag
    27901 cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa
    27961 aaacggaaca gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta
    28021 cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac
    28081 aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat
    28141 gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga
    28201 aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca
    28261 aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac
    28321 tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa
    28381 gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct
    28441 cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata
    28501 tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga
    28561 gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga
    28621 caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga
    28681 ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct
    28741 tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc
    28801 taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc
    28861 ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat
    28921 tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc
    28981 caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg
    29041 ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat
    29101 tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc
    29161 atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg
    29221 tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg
    29281 gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt
    29341 tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag
    29401 gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg
    29461 agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat
    29521 ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa
    29581 gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg
    29641 ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag
    29701 ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa
    29761 gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa
    29821 gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa
    29881 tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga
    29941 ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat
    30001 ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct
    30061 agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc
    30121 tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac
    30181 cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta
    30241 caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg
    30301 gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa
    30361 atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct
    30421 gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat
    30481 ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat
    30541 tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag
    30601 gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca
    30661 ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct
    30721 ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct
    30781 ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc
    30841 tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga
    30901 ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg
    30961 aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc
    31021 gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga
    31081 tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca
    31141 taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag
    31201 tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta
    31261 cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt
    31321 taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc
    31381 ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc
    31441 agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt
    31501 cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt
    31561 aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga
    31621 agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa
    31681 aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc
    31741 gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac
    31801 gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat
    31861 tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga
    31921 tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc
    31981 aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg
    32041 agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact
    32101 ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca
    32161 aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt
    32221 caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc
    32281 aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta
    32341 aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt
    32401 ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca
    32461 gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta
    32521 ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa
    32581 gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg
    32641 gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg
    32701 attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc
    32761 ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta
    32821 tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa
    32881 aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt
    32941 gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc
    33001 gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg
    33061 ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt
    33121 gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct
    33181 gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca
    33241 agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca
    33301 gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg
    33361 gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa
    33421 aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg
    33481 aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta
    33541 accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac
    33601 aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca
    33661 tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca
    33721 ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac
    33781 gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag
    33841 atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta
    33901 aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa
    33961 tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg
    34021 gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc
    34081 aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta
    34141 ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat
    34201 acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac
    34261 cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt
    34321 tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg
    34381 tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg
    34441 gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa
    34501 atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa
    34561 ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa
    34621 acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag
    34681 agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat
    34741 tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca
    34801 gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa
    34861 gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat
    34921 tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt
    34981 tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct
    35041 ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt
    35101 gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg
    35161 acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct
    35221 cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt
    35281 gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa
    35341 ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct
    35401 gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt
    35461 ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca
    35521 tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg
    35581 ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc
    35641 attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca
    35701 acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa
    35761 atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca
    35821 gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga
    35881 gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa
    35941 gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa
    36001 taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca
    36061 ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa
    36121 atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga
    36181 aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa
    36241 gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga
    36301 ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa
    36361 agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc
    36421 accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt
    36481 tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg
    36541 tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag
    36601 tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt
    36661 tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt
    36721 taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc
    36781 aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga
    36841 atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt
    36901 cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac
    36961 atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac
    37021 ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta
    37081 tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag
    37141 aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg
    37201 gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg
    37261 acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac
    37321 tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct atttctatag
    37381 cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct
    37441 aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt
    37501 gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt
    37561 acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca
    37621 acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca
    37681 gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt
    37741 tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt
    37801 agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca
    37861 ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt
    37921 tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa
    37981 aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc
    38041 ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca
    38101 agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat
    38161 tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag
    38221 tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg
    38281 taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga
    38341 tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa
    38401 aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta
    38461 ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga
    38521 tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg
    38581 actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac
    38641 ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat
    38701 aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa
    38761 gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa
    38821 gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa
    38881 actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag
    38941 tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc
    39001 tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa
    39061 aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat
    39121 acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa
    39181 gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg
    39241 tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac
    39301 ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca
    39361 cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga
    39421 gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg
    39481 cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc
    39541 taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct
    39601 ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt
    39661 atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca
    39721 catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc
    39781 atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga
    39841 gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat
    39901 ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt
    39961 tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct
    40021 gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac
    40081 ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca
    40141 ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc
    40201 gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt
    40261 tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca
    40321 agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac
    40381 gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt
    40441 agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa
    40501 gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta
    40561 aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg
    40621 cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa
    40681 ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa
    40741 acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca
    40801 tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa
    40861 acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa
    40921 gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt
    40981 acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt
    41041 ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa
    41101 tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca
    41161 tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa
    41221 tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt
    41281 cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta
    41341 agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat
    41401 acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac
    41461 aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac
    41521 aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg
    41581 tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa
    41641 cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact
    41701 cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac
    41761 gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat
    41821 acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga
    41881 aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa
    41941 gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca
    42001 gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat
    42061 gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt
    42121 cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc
    42181 gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa
    42241 gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg
    42301 ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc
    42361 gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg
    42421 gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg
    42481 agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg
    42541 gctattttca accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa
    42601 tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata
    42661 gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt
    42721 gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa
    42781 acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa
    42841 tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata
    42901 gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc
    42961 taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg
    43021 gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa
    43081 aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc
    43141 ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca
    43201 ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt
    43261 tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg
    43321 gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt
    43381 gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa
    43441 ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa
    43501 tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg
    43561 atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat
    43621 tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat
    43681 tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa
    43741 tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt
    43801 agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag
    43861 ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg
    43921 tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat
    43981 tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat
    44041 gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga
    44101 caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca
    44161 tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact
    44221 aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt
    44281 gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta
    44341 gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg
    44401 ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt
    44461 ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa
    44521 aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac
    44581 aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata tatatataca
    44641 ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa
    44701 agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa
    44761 cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg
    44821 taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct
    44881 tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa
    44941 cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca
    45001 ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga
    45061 ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt
    45121 atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat
    45181 agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga
    45241 aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc
    45301 tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc
    45361 tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat
    45421 tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat ttacggtata
    45481 caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg
    45541 acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt
    45601 tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca
    45661 agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac
    45721 gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg
    45781 agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc
    45841 cattccattt gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat
    45901 aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc
    45961 tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca
    46021 ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga agactttttt
    46081 caatattact catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca
    46141 agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac
    46201 gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga
    46261 agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa
    46321 agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt
    46381 attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga
    46441 tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca
    46501 ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta
    46561 tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga
    46621 gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca
    46681 atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc
    46741 aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg
    46801 gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga
    46861 tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac
    46921 gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc
    46981 tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta
    47041 gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac
    47101 gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt
    47161 caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg
    47221 gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg
    47281 attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa
    47341 gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc
    47401 attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca
    47461 gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac
    47521 gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata
    47581 cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt
    47641 ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta
    47701 ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa
    47761 ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat
    47821 attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg
    47881 catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa
    47941 gataataatc taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag
    48001 atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct
    48061 gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac
    48121 aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca
    48181 aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc
    48241 agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc
    48301 aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa
    48361 gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag
    48421 tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta
    48481 cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc
    48541 aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa
    48601 ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga
    48661 tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgat caaaatccgc
    48721 aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag
    48781 atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc
    48841 gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc
    48901 cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg
    48961 aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc
    49021 cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac
    49081 aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca
    49141 tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg
    49201 caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag
    49261 aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg
    49321 accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg
    49381 tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca
    49441 cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg
    49501 tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc
    49561 ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt
    49621 tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat
    49681 tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa
    49741 attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac
    49801 aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag
    49861 gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac
    49921 agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag
    49981 cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg
    50041 tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat
    50101 taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga
    50161 tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag
    50221 aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag
    50281 cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg
    50341 aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag
    50401 aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat
    50461 tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc
    50521 catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag
    50581 ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg
    50641 tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg
    50701 atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca
    50761 tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct
    50821 caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa
    50881 aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa
    50941 ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc
    51001 actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat
    51061 gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa
    51121 acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa
    51181 aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca
    51241 gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc
    51301 atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat
    51361 atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg
    51421 aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag
    51481 ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg
    51541 cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat
    51601 cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc
    51661 atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt
    51721 ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac
    51781 aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag
    51841 gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag
    51901 ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt
    51961 tgctttgcgt gatgctttta tcggcatact gcccgcaaca atggcgggat cagtcgctgt
    52021 aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag
    52081 tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt
    52141 tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa
    52201 tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt
    52261 gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc
    52321 aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag atggcacact
    52381 gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac
    52441 agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat
    52501 tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt
    52561 accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc
    52621 tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt
    52681 atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt
    52741 tggtattcat ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca
    52801 actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt
    52861 gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt
    52921 tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc
    52981 taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat
    53041 caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt
    53101 cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt
    53161 cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc
    53221 aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt
    53281 gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca
    53341 ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag
    53401 gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt
    53461 tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc
    53521 tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa
    53581 tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg
    53641 tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt
    53701 ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg
    53761 tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag
    53821 aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc
    53881 gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa
    53941 atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa
    54001 ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc
    54061 gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg
    54121 tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat
    54181 gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat
    54241 gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag
    54301 taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt
    54361 tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt
    54421 gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga
    54481 gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat
    54541 cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac
    54601 ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc
    54661 aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat
    54721 ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa
    54781 atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata
    54841 aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg
    54901 gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa
    54961 aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc
    55021 gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat
    55081 gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa
    55141 tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct
    55201 gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat
    55261 tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg
    55321 aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa
    55381 gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta
    55441 ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa
    55501 tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt
    55561 tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat
    55621 ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt
    55681 ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat
    55741 tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg
    55801 gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt
    55861 ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa
    55921 acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat
    55981 cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg
    56041 tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta
    56101 tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc
    56161 tctcgaacaa tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    56221 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt
    56281 agtccatgca aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat
    56341 ttgctgacgg cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga
    56401 gggtttttga tttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag
    56461 agatcaatcg tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt
    56521 ccccattcat ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa
    56581 tcgatatctt cccatgttaa tcctagaagt ccagcaaatc gcattccagt ggcaccagca
    56641 actaacacga gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt
    56701 aaagatttaa attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt
    56761 cctttgatta ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct
    56821 ttcaacgacg ctctaatatg gttattaaac ttaataacgg tagattttac atggtcttta
    56881 gcatacttat ttaaaaagcg ttgatatccc actctatcta aatcagaaat caataccgct
    56941 ggcatatatt tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat
    57001 actgttcctt ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt
    57061 ttgcgatccc ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt
    57121 gcttctcctt ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt
    57181 ttgtatgaaa tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa
    57241 tcagctctct ttctctgata caataggaac tataaagaag cccattgttt gggttttatt
    57301 attttttgca catccttttt gcttgccggc gcaaggatgt gctatttgtc gttttaatgg
    57361 tataattgga tgtgtattag atcatccaat cagaggtgtt tattatgatt aaagtgattt
    57421 ttaaaagaat aactgaactt ccagagttta gattatttgt tgattattgg tatgtctggt
    57481 tttttattgt tgcattgctg ataggtctag atgtcatatt agaaagaaaa cttaagtaag
    57541 agttgagtta ataaattgaa ttcctaaatg agatattttg tagcccatat tttgtttgcc
    57601 gacatcacct aatactatgg agctaatcgt tttttctaac agaccaaaat cagataatct
    57661 atttaagcta ccatctatta gctcttgtga atagccagac atgctgatat ttccagtgaa
    57721 attttcgttt ccacgactca ttaagcatgc taacaattga atctcaatcg atgttaattg
    57781 attcagtaca agaagataat actcctcgtt atcccatgaa gcgttgttaa ttcgaagtaa
    57841 acaattctta taaagatttt tatacatatc tattttattt tgtgcctttg atttttctac
    57901 ctcgtcattt atcccttcga atattccaat gagttggtcc ttgttctcta agttgaaaac
    57961 atcttctgga atttgatcgc gaatgttttc taatgattcg tttaaatttt tgtagaattt
    58021 ctcgatgcgt ttgaaacgct tctcgttttt agacccaaaa taaagtgact gcacaacgcc
    58081 accaatataa ggaattgctc caataccgcc ttcaactatt aattcaagat attctttttt
    58141 ggttagtttt tcattgctat tatccatagt tacctcctat tttgtcattt cctttacaag
    58201 cattgtgact cctgatggca ctcttttgta taggttttat tattgtgcac aatctaactc
    58261 ttagtggggc gtggttcatt tattttttac ttactttact attaatcata tcccattgag
    58321 cataggattt ttctatgttc gatcctcttg aactaatttc tcctgtaaaa gttactttat
    58381 cgccctttgt aaaggtatca gcattgttta agtccttcgc aaaaattaca tatgaaactt
    58441 taggtgttgc agaaatatca ttccaacttt tatcatcgta ctctttgtct gctataatgg
    58501 ctattctagt agtcattgat tcgataacag ttccttcaaa agtatatgtt ttaccactaa
    58561 tatatttagt aaaagctgta ttttgctcag tagctggagt aatagaataa taactatcta
    58621 agaaagcacc ccagtcatga ttatttgtct ctaaaaattc ttcaaattga gttaggcttt
    58681 tattgctgct gttagatgta gtagtttgag tatcagatga tgagctttcc tgtttgttat
    58741 tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac ggtagtaaaa ataatcccga
    58801 taataaggag aatcataggt gttttcttat ttgttttttt gataaatctt acaattaaca
    58861 tgattaaacc tataataaat aaaacaatac cagtaaaacc aaatataccg tccattaata
    58921 gatcctcttt ctcttttgat attgaatatt ataaaccaag caattctttt ttcttaaaat
    58981 cgaattcttc ttgattaata atcccttcat ctaacaactc tttatacttt ttaatttcat
    59041 ctgcacttga aatttgtgtt tctttggaat tattatctaa tatagattgt tgcttctcta
    59101 tatatttttt taaattttcc atttcattcc aatatttttt tgtaaacata acggtgtttt
    59161 catctttcgt ggccgctaat atacctccgc gactttcgtt tccgcctagc aaggtaaatt
    59221 gtatataacc attactcata cctggttttt ttaattgtac ggctgatata tttttaaagg
    59281 ggatagtttt ttctccttta ataccttgat taactaaatt tataaatcct tttctaataa
    59341 ttgaaataga atcatcagtt actttaatca aggtttttcc tggagatttt attaaaatct
    59401 ctttttccat tttttcttcc tcatttctgt gatatgattt tttttgtaga agctcatata
    59461 tgaggaaaga gcccgtgttg cagcacgggc ttttttaatg ttttgctgaa atcggttttt
    59521 ttaataattc ttgatgtatt catacatatt gccttgcgta agaatatttt ttcttaaaat
    59581 agcattggca gacagcataa caagtgtatc tgagcttatt ctgaatagaa taatactcca
    59641 taaatttttc gagattaaac tgagattcat cagtcagttc gttctcaata tagatattta
    59701 ataaaattag aatagctatt ttatctgctt ctgtttcaaa ttttgagtga aaagttgtag
    59761 aagtatcgta caaaactgaa aattcaaaat gagaggcgct gaaatgtgca agttcatgag
    59821 ataaatgaaa ggcttctgca gtttcaccgt atagattttc attcaaaaaa atgattctgg
    59881 gttttggata gtagaaacca ggctctttca tctccatata aactactttc aaattgtatt
    59941 cgctcaacat ttctttcaat ttcaaataca tacaaaccat cactccaact attcattttc
    60001 ctctaaagct ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc
    60061 atcaaaagaa aaaacagtat cgtcttctga taaatccaca tgtttagggg tttctctttc
    60121 ttctctacct agaaggtagt ctacagagac atcgaaatag tcagcaatcc gacttaattc
    60181 atctgaatta ggagtattat ttttccactt tgctagataa ccgttagaat atccaaaatt
    60241 aatttctaac tgccgtatag atatcttttt cctcttcgcc aagtctttta ttatttcata
    60301 ggtattcatc gataaaacaa cctttctaaa tgtttacaaa aaaagtttag aaaaatacgc
    60361 agaaataatt tgactaattc tgagtaatga gctatactat atcttgtaaa cagatttaac
    60421 aactaaaaag acaacaaaaa acactattga tatgtaaatg cagaccgcca agaaagcttt
    60481 aaaatcaatg tttttatgtc ttatttaatt atgctcttag tatagagtat tactcagata
    60541 ctgtcaattg aatttagaaa aaagttgtta aatttgttta cgaatataaa agaaaggaat
    60601 gagaaatatg aacacaccgc aaatttttaa tttcgaacaa aacgaagttc ggacaatttt
    60661 agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg gttattcaaa
    60721 cactaaagat gctttgtcgc ggcatgtaga tttggaagat aagatggggt cgcgaatcac
    60781 gacctcaggt caatcaagag agatgacaat catcaacgaa tctggtttgt acagtttaat
    60841 cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa gtgaagtgtt
    60901 gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag aagctttgct
    60961 taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa ctggaagatt
    61021 aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag atagcatatt
    61081 atcttctaca gattcagtaa caattagtca gattgcagca gattatggga tgtctccaca
    61141 acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta accaatggtt
    61201 attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg agatcccgaa
    61261 agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag ggcgtctatt
    61321 tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag aggaaattgg
    61381 ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga tagagaaaaa
    61441 atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc tgaaagattt
    61501 ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc tgcagaagca
    61561 aacagaatta tagattcgat tatcactatg tacgaattgt aaagggagga acagctaatg
    61621 caatatctag aagcaaaaat cccaattcca gaaggctatg taattatctc ccaagtggat
    61681 tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc agaagtgcta
    61741 gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa cccaagatat
    61801 agaaatatta tcgatatcga gaaaaacaaa aatggatttg tctattatcc agttgaagga
    61861 agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac aaatttttcg
    61921 gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtgt tcagcgcacc
    61981 gctaatcatc tacgctttag gactttgggg aagcag
//
LOCUS       DASJRQ010000004        61712 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJRQ010000004 DASJRQ010000000
VERSION     DASJRQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.004"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..509)
                     /locus_tag="U4A80_000210"
     CDS             complement(132..509)
                     /locus_tag="U4A80_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973882.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="U4A80_000211"
     CDS             765..1493
                     /locus_tag="U4A80_000211"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1973883.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="U4A80_000212"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="U4A80_000212"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1973884.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="U4A80_000213"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="U4A80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1973885.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="U4A80_000214"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="U4A80_000214"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1973886.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="U4A80_000215"
     CDS             4628..6067
                     /locus_tag="U4A80_000215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1973887.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="U4A80_000216"
     CDS             6090..7142
                     /locus_tag="U4A80_000216"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1973888.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="U4A80_000217"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="U4A80_000217"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1973889.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="U4A80_000218"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="U4A80_000218"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1973890.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="U4A80_000219"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="U4A80_000219"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1973891.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="U4A80_000220"
     CDS             10661..11575
                     /locus_tag="U4A80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1973892.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="U4A80_000221"
     CDS             complement(11708..13273)
                     /locus_tag="U4A80_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1973893.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="U4A80_000222"
     CDS             complement(13266..14717)
                     /locus_tag="U4A80_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1973894.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="U4A80_000223"
     CDS             complement(14731..15747)
                     /locus_tag="U4A80_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1973895.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="U4A80_000224"
     CDS             15967..17055
                     /locus_tag="U4A80_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1973896.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="U4A80_000225"
     CDS             17109..18635
                     /locus_tag="U4A80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1973897.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="U4A80_000226"
     CDS             18637..19842
                     /locus_tag="U4A80_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1973898.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="U4A80_000227"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="U4A80_000227"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1973899.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="U4A80_000228"
     CDS             21340..22764
                     /locus_tag="U4A80_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1973900.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="U4A80_000229"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="U4A80_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1973901.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="U4A80_000230"
     CDS             24337..25314
                     /locus_tag="U4A80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1973902.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="U4A80_000231"
     CDS             25339..26982
                     /locus_tag="U4A80_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN1973903.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="U4A80_000232"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="U4A80_000232"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1973904.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="U4A80_000233"
     CDS             28558..28833
                     /locus_tag="U4A80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1973905.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="U4A80_000234"
     CDS             28839..29606
                     /locus_tag="U4A80_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1973906.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="U4A80_000235"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="U4A80_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1973907.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="U4A80_000236"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="U4A80_000236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1973908.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="U4A80_000237"
     CDS             33750..34052
                     /locus_tag="U4A80_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1973909.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="U4A80_000238"
     CDS             34055..35218
                     /locus_tag="U4A80_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1973910.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="U4A80_000239"
     CDS             35244..38672
                     /locus_tag="U4A80_000239"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1973911.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="U4A80_000240"
     CDS             38693..39817
                     /locus_tag="U4A80_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973912.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39807..40244
                     /locus_tag="U4A80_000241"
     CDS             39807..40244
                     /locus_tag="U4A80_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1973913.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="U4A80_000242"
     CDS             40228..40566
                     /locus_tag="U4A80_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1973914.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="U4A80_000243"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="U4A80_000243"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1973915.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="U4A80_000244"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="U4A80_000244"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1973916.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="U4A80_000245"
     CDS             41723..42766
                     /locus_tag="U4A80_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1973917.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="U4A80_000246"
     CDS             42925..43653
                     /locus_tag="U4A80_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1973918.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="U4A80_000247"
     CDS             43769..44014
                     /locus_tag="U4A80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1973919.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="U4A80_000248"
     CDS             44011..44229
                     /locus_tag="U4A80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1973920.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="U4A80_000249"
     CDS             44365..44904
                     /locus_tag="U4A80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1973921.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="U4A80_000250"
     CDS             44897..47170
                     /locus_tag="U4A80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1973922.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="U4A80_000251"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="U4A80_000251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1973923.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /locus_tag="U4A80_000252"
     CDS             complement(48343..48597)
                     /locus_tag="U4A80_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1973924.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="U4A80_000253"
     CDS             complement(48997..49266)
                     /locus_tag="U4A80_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973925.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49736..50191)
                     /locus_tag="U4A80_000254"
     CDS             complement(49736..50191)
                     /locus_tag="U4A80_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1973926.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="U4A80_000255"
     CDS             complement(50329..52257)
                     /locus_tag="U4A80_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1973927.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="U4A80_000256"
     CDS             complement(52454..53287)
                     /locus_tag="U4A80_000256"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1973928.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="U4A80_000257"
     CDS             complement(53479..54183)
                     /locus_tag="U4A80_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1973929.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="U4A80_000258"
     CDS             complement(54206..54832)
                     /locus_tag="U4A80_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1973930.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="U4A80_000259"
     CDS             55158..57533
                     /locus_tag="U4A80_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1973931.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="U4A80_000260"
     CDS             complement(57557..58048)
                     /locus_tag="U4A80_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1973932.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="U4A80_000261"
     CDS             58235..59155
                     /locus_tag="U4A80_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1973933.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="U4A80_000262"
     CDS             59309..59653
                     /locus_tag="U4A80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1973934.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="U4A80_000263"
     CDS             complement(59843..60343)
                     /locus_tag="U4A80_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1973935.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="U4A80_000264"
     tRNA            60871..60954
                     /locus_tag="U4A80_000264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="U4A80_000265"
     CDS             complement(61278..>61712)
                     /locus_tag="U4A80_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1973936.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20241 a        10663 c        13217 g        17591 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DASJRQ010000005        59163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJRQ010000005 DASJRQ010000000
VERSION     DASJRQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.005"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(77..517)
                     /locus_tag="U4A80_000266"
     CDS             complement(77..517)
                     /locus_tag="U4A80_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1973937.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="U4A80_000267"
     CDS             complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="U4A80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1973938.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1572..2945)
                     /locus_tag="U4A80_000268"
     CDS             complement(1572..2945)
                     /locus_tag="U4A80_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1973939.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="U4A80_000269"
     CDS             complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="U4A80_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1973940.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3771..4103)
                     /locus_tag="U4A80_000270"
     CDS             complement(3771..4103)
                     /locus_tag="U4A80_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1973941.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4155..4784)
                     /locus_tag="U4A80_000271"
     CDS             complement(4155..4784)
                     /locus_tag="U4A80_000271"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1973942.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="U4A80_000272"
     CDS             complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="U4A80_000272"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1973943.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5629..8274)
                     /gene="polA"
                     /locus_tag="U4A80_000273"
     CDS             complement(5629..8274)
                     /gene="polA"
                     /locus_tag="U4A80_000273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1973944.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQLTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8400..9083)
                     /locus_tag="U4A80_000274"
     CDS             complement(8400..9083)
                     /locus_tag="U4A80_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1973945.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9076..9561)
                     /locus_tag="U4A80_000275"
     CDS             complement(9076..9561)
                     /locus_tag="U4A80_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1973946.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9868..11202)
                     /locus_tag="U4A80_000276"
     CDS             complement(9868..11202)
                     /locus_tag="U4A80_000276"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1973947.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11333..12094)
                     /locus_tag="U4A80_000277"
     CDS             complement(11333..12094)
                     /locus_tag="U4A80_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1973948.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12110..12592)
                     /locus_tag="U4A80_000278"
     CDS             complement(12110..12592)
                     /locus_tag="U4A80_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1973949.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12612..14792)
                     /locus_tag="U4A80_000279"
     CDS             complement(12612..14792)
                     /locus_tag="U4A80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1973950.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(14936..15667)
                     /locus_tag="U4A80_000280"
     CDS             complement(14936..15667)
                     /locus_tag="U4A80_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1973951.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15736..16677)
                     /locus_tag="U4A80_000281"
     CDS             complement(15736..16677)
                     /locus_tag="U4A80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1973952.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16857..17225)
                     /locus_tag="U4A80_000282"
     CDS             complement(16857..17225)
                     /locus_tag="U4A80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1973953.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(17836..19227)
                     /locus_tag="U4A80_000283"
     CDS             complement(17836..19227)
                     /locus_tag="U4A80_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1973954.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19440..19826)
                     /locus_tag="U4A80_000284"
     CDS             complement(19440..19826)
                     /locus_tag="U4A80_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1973955.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(19882..20505)
                     /locus_tag="U4A80_000285"
     CDS             complement(19882..20505)
                     /locus_tag="U4A80_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1973956.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20569..21237)
                     /locus_tag="U4A80_000286"
     CDS             complement(20569..21237)
                     /locus_tag="U4A80_000286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1973957.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21249..21689)
                     /locus_tag="U4A80_000287"
     CDS             complement(21249..21689)
                     /locus_tag="U4A80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973958.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21748..22419)
                     /locus_tag="U4A80_000288"
     CDS             complement(21748..22419)
                     /locus_tag="U4A80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1973959.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(22604..23002)
                     /locus_tag="U4A80_000289"
     CDS             complement(22604..23002)
                     /locus_tag="U4A80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1973960.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(22999..23772)
                     /locus_tag="U4A80_000290"
     CDS             complement(22999..23772)
                     /locus_tag="U4A80_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1973961.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23769..24452)
                     /locus_tag="U4A80_000291"
     CDS             complement(23769..24452)
                     /locus_tag="U4A80_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1973962.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24619..25506)
                     /locus_tag="U4A80_000292"
     CDS             complement(24619..25506)
                     /locus_tag="U4A80_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1973963.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25503..26372)
                     /locus_tag="U4A80_000293"
     CDS             complement(25503..26372)
                     /locus_tag="U4A80_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1973964.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26509..27795)
                     /locus_tag="U4A80_000294"
     CDS             complement(26509..27795)
                     /locus_tag="U4A80_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1973965.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28068..28646
                     /locus_tag="U4A80_000295"
     CDS             28068..28646
                     /locus_tag="U4A80_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1973966.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28646..29575
                     /locus_tag="U4A80_000296"
     CDS             28646..29575
                     /locus_tag="U4A80_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1973967.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29572..31308
                     /locus_tag="U4A80_000297"
     CDS             29572..31308
                     /locus_tag="U4A80_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1973968.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="U4A80_000298"
     CDS             complement(31371..32207)
                     /gene="pheA"
                     /locus_tag="U4A80_000298"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1973969.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32204..32713)
                     /locus_tag="U4A80_000299"
     CDS             complement(32204..32713)
                     /locus_tag="U4A80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1973970.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="U4A80_000300"
     CDS             complement(32706..34001)
                     /gene="aroA"
                     /locus_tag="U4A80_000300"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1973971.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34014..35105)
                     /locus_tag="U4A80_000301"
     CDS             complement(34014..35105)
                     /locus_tag="U4A80_000301"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1973972.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="U4A80_000302"
     CDS             complement(35122..36288)
                     /gene="aroC"
                     /locus_tag="U4A80_000302"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1973973.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="U4A80_000303"
     CDS             complement(36290..37354)
                     /gene="aroB"
                     /locus_tag="U4A80_000303"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1973974.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="U4A80_000304"
     CDS             complement(37387..38409)
                     /gene="aroF"
                     /locus_tag="U4A80_000304"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1973975.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(39068..40042)
                     /locus_tag="U4A80_000305"
     CDS             complement(39068..40042)
                     /locus_tag="U4A80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1973976.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(40023..41906)
                     /locus_tag="U4A80_000306"
     CDS             complement(40023..41906)
                     /locus_tag="U4A80_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1973977.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(41924..44149)
                     /locus_tag="U4A80_000307"
     CDS             complement(41924..44149)
                     /locus_tag="U4A80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1973978.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            44264..45298
                     /locus_tag="U4A80_000308"
     CDS             44264..45298
                     /locus_tag="U4A80_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1973979.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(45365..46378)
                     /locus_tag="U4A80_000309"
     CDS             complement(45365..46378)
                     /locus_tag="U4A80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1973980.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(46456..46644)
                     /locus_tag="U4A80_000310"
     CDS             complement(46456..46644)
                     /locus_tag="U4A80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1973981.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(47084..47212)
                     /locus_tag="U4A80_000311"
     CDS             complement(47084..47212)
                     /locus_tag="U4A80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1973982.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="U4A80_000312"
     CDS             complement(47215..48588)
                     /gene="rlmD"
                     /locus_tag="U4A80_000312"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1973983.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(48699..49754)
                     /locus_tag="U4A80_000313"
     CDS             complement(48699..49754)
                     /locus_tag="U4A80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1973984.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="U4A80_000314"
     CDS             complement(49764..51194)
                     /gene="gatB"
                     /locus_tag="U4A80_000314"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1973985.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="U4A80_000315"
     CDS             complement(51191..52663)
                     /gene="gatA"
                     /locus_tag="U4A80_000315"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1973986.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="U4A80_000316"
     CDS             complement(52663..52968)
                     /gene="gatC"
                     /locus_tag="U4A80_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1973987.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="U4A80_000317"
     CDS             complement(53074..55110)
                     /gene="ligA"
                     /locus_tag="U4A80_000317"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1973988.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="U4A80_000318"
     CDS             complement(55127..57361)
                     /gene="pcrA"
                     /locus_tag="U4A80_000318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1973989.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            57516..59075
                     /locus_tag="U4A80_000319"
     CDS             57516..59075
                     /locus_tag="U4A80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1973990.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        16202 a        12869 c        10508 g        19584 t
ORIGIN      
        1 tcccatatct ccttaaaaaa tatacctttt cgttctataa aacaacattt tcttattttt
       61 gaaaaaatgt tattggttat ttgttcaatg aaacatcaat aatcgaatct ccaggaactt
      121 gcggtgggat agacatcgtc atgatcggca tctcaactaa tgtaaattgg actttgtcac
      181 cgattttcaa ttcatttatg tccacgctta ttttttcagc agtagcattt aagatcactc
      241 cgtcattgct gaatgacgtt cccatattcg ctggatcttc tttggccatg acatcgacga
      301 gtttcaactg aagcgtttct tcttctgctt ctacttgagt gatactttca atcgttccga
      361 caaatatgct ttgtctctcc gttttcgtac tgcttgtttt tactgtttga cccgcatcaa
      421 tcgaatcaac agaacctgtg ttgctatctg cgctaccgca gcctgctaaa agaaaaaata
      481 gaccaactac agatatacta gaaaaaatag ccctcataaa aaatcctccg tttaagtttc
      541 tgcattactt gttgcggtga ttgtctccgg tcatttctgt ccatataatg attcgctgac
      601 ctgcttttac tactaggtca aatgtcagtt ggacttgtca actgttcaac cattgcgtcg
      661 gttgcgacca gtcatttcga tttcttttgt caaataacgt atgcgttcca ttatccgttt
      721 cgcttttagc ggctcttcat ctccacgttg agtgacggac aaatgctgtt ccaattcatc
      781 cattgtaaaa ttcgatgaga agaaggttgg taactgttcc tgcatccgat attgaaggat
      841 aacaccgaat acttcgtctc gtatccaact actcatcgca tctgcaccaa tgtcatcgat
      901 catcaaaata ggtgaacgtt tcactgcatc taacttttct gccacttgat cttttccgat
      961 tgcctgtttc atctcaacgg caaatgtcgg aaaatggact agcgtcgtgg tgaatccagc
     1021 aatcgctaat tctctcgctg ttgctcctaa taaatatgtt tttccaatac caaaacttcc
     1081 tactagatac agtcctttat gatacccttt tggatcagca gtatatttct ctacaaaatc
     1141 tagggcttct aagctcgcat atcctcgtcc atctgtacgt tcaaagtttt caaaagaagc
     1201 cgattgaata tcttttggca tatccattgc ttttactcgg cttctgattt cttcttgtct
     1261 ttgccgtgct aacaatgctt ccgttggtac ataagtgaca tccacataat gaaaattcaa
     1321 cgtcagcttc ggctcatagc cgggagcgat catcgttgga tcgttttttt gatatttacg
     1381 cttttcttgc acgaattcat acaatttagc gtaacttttt tcaatatccg cttgtgtcaa
     1441 ttcttcttgg tgtgcgctta gaaaagcttg aacatccgca tcatttaaga cttcagcgat
     1501 catttcagca tacttttctt gataattgcg tttgctgatg atcctcttca attctttgcc
     1561 tacgtcttcc actagatatc accctccttt tttgccaggt attctttaaa acggcggtca
     1621 atctctgctt ttttctcgct gctgatctgt ttttcatctt ttggctggtt gacccaatca
     1681 ggaagctttt cccgtcgaac atttcgcttg ccagtttgat aatttgtttg cttcttttgt
     1741 tttgattgtt ttgcttgatt acttatttgc cgaatgtgct ggatcgcttc ttctgctgtg
     1801 gcgattttct tttgtgccca ttcatttgcc actgtattta cataactggc atttaagcta
     1861 gcattattct tgatcactag tacataattc agcaaaacat tgatcacgct actggaaaga
     1921 cctgattgag aaacaagatc ttgcagtaac cagttttctt gtttagaaac atatccgcct
     1981 ttttctgact tgattgcttc cagatatttc atcggtggaa tgctctgtgc ttcttttatc
     2041 agctgaagcg ctgcgtttgt caaatgttcg tttgacgagg gttcattact gttttcagaa
     2101 accgcaactg tttcttttgt ttgcttcacc ggacgtctct gcattgccaa aatggctttt
     2161 tgcagttctt tttcagatac tttttgttca ctaaagtcat aagattgact gatcagttcg
     2221 ccaagtgtca attcatcgaa accgtaaagc tgatgataca tgtcgacttg tttgatgatt
     2281 ggatcatcaa gacttgcaat atgttttttt gccagccaat cattcaaaaa ctgccaatcg
     2341 aatgtcgaat ctgcttcaag taatgctact gattcagatt cttcaaaaga tttattcagc
     2401 gattcgatgt tttcttgatt agcagcgtac actgcttcat tgaagcgata aacatctaaa
     2461 aatttttttg tgattttttg atatccctct gctgtatgcc ttttaggttc aaagaattgg
     2521 gtaagcttct ggaatcgtcg ttcaccaact ttttccagaa gaagaaaagc cagaacagag
     2581 tctttgaaaa atgctttagg aatgagtggt tctaacagct cataaataaa acgaggtccc
     2641 agatcactat cttctttaaa ataaacatcc agtaagccga ttccttctag cttttttctt
     2701 gcatcataaa attgcgggat accgcagcct agactactaa gcagatctgc atgcatccct
     2761 tcaggacctt tgccagattc ttgagagatt tctgataaca gggtgaaata gatactcaag
     2821 gcttctccgc cgattagtgg ctgataaaga tacagcaagg cgttcttgct gtcttctctc
     2881 agcggagccg cttttcttcc ttggtaaata ttttttggtt gaagctcttt ccaagcattt
     2941 tccaaggaaa tgcctccttt ttgtttctta attgttttct ttattattct ctttattatt
     3001 ttcttttgct ttatccacga tatcttgcaa ctcttttaag aaaacagaca tatctttgaa
     3061 ctggcgataa acacttgcaa aacgaatata agcgatttcg tctaaatcaa ctaaatcttc
     3121 catcacatat tcaccaatta gattcgtcga tacttcgttt tctccgagac tgcgtacgcg
     3181 gttttcgaca tggttgacga tttgttccat ttgttccata gctacaggtc ttttttctgc
     3241 tgaacggatg agtcctctta aaattttatc gcgattgaac tcttcgcgtt ccccgttttt
     3301 cttgattact aataacggag cagcttcaat tcgttcaaat gtagtgaatc ggaacgaaca
     3361 attctcacat tcgcgacggc gacggatcgc acgtccatca tctgcttgac ggctgtcgat
     3421 tactcgtgaa ttattatgtt gacatctggg acatctcatt ttgtctcacc tcttgcatcg
     3481 cattttatat aaaggatgtg tctttcttta caactagttg tattataaca cgaaacaaat
     3541 cacaaacgcg actgtcctac ccaaaaaaca cggatataag aacaattttt tctactttca
     3601 tgcgtaattg tgtcccctgt ccttactaaa tttcaatttt ccaatactga tcaccttgat
     3661 tactgagaaa taacttacac aaaagctgat ttagtcatct agcacaaaaa acttgaagca
     3721 gatgaacacc ttctcatctg cttcaagtca catctcgatt ttttactatt ttataacttc
     3781 tcttcgattc ctgtatagtg tttcattcgc tgttcctcga ttttttgctc atcgggaatg
     3841 tggtcacttt ctgggagcgg tgttttacta cgatcaggtg tcggattttt tcgttcttga
     3901 gtcaaatctt gccgcttcgc ttcttcttct gcctgctggt cttctatttc atcgattctt
     3961 gcttggtact gcttttcttt ttcttctgct gtttctgatt gcgtacgacc ttcatcgatt
     4021 agattttctt tattgagcat ctcgccgatt ttttcttttg cgttacctac gactttatcc
     4081 ttttgatttt ttaatgatcc catcgctatc cctcactttc tatatactca ggatagcaaa
     4141 attgacacag atactcaaag aaagcgatta gcaaaaagcc aattttcgat ttgtcccaca
     4201 agtttttctt ttgttccatt gttgtcgaac aaaatatccg cacgctgctt tttttcttcg
     4261 attggaagtt gactactaat tctctgctgc gcttgttcta gcgtcagttg attacgtttc
     4321 atcaaacgct gaagctgagt ttctggcgta gtataaacga cagctactgc atccatatag
     4381 tggtcataat gaccttcgta gagcaatggg atatctacaa ttactaaagg atgattggaa
     4441 cttagagagg cgatttgatc ttttatcgct tttcggataa acggatctaa tagctgatca
     4501 agtttttgcc gtttttcctt tgatggaaaa acaatcatcc caagtttccc tcgatctagt
     4561 tgcccatcag gctgcaatac ctcttggcca aacactgata caattttcct cagagcaagc
     4621 gttccaggct caacagtttc tctggcaatc acatcgccat cgatcaaagg aaatccatga
     4681 gactgaaaga ctttggctgc cgtactttta ccggtagcta ttccacccgt caaacctaaa
     4741 acaaatccta ctttgcgatt tttagccttt gacaattcgg acaatagtgc gtcccccttt
     4801 gtgccacttt tgttttcacg atcggcgttc cacaacgtat acatggtttg ccagtttgct
     4861 gatagacatg cagtgctact tgaaatccgc ctgattctcc taatgcattc aaataactgc
     4921 gaatcgtggt acctccggct tctattgcac gatccaaaac atcaatgatc gatagacgta
     4981 attgttctac ctctttgggg cgcagtgtgt tcgccggttg ttctggatgg atcttcgctt
     5041 cccataatgc ttcatctaca taaatattac ctagaccagt cactagacgt tggtctagca
     5101 gcaatggctt gattgctttg tgggactttt tcagtccatt tgcgaaatct gctaataaga
     5161 agctatctgg caaaggctca ggtcccaatt tcatgattcc tcggtaagtt gcacttgctc
     5221 ctttttcgac gattgtcata cgaccaaatt ttcgtacatc gttgtagcga agttggcttc
     5281 catcctcaaa aaagaaaagt acatgcgtat gtttatcgat tggtgtattt tctttcgtaa
     5341 attgatattt tccttccatt cgcaagtgtg agatcagttc acaatgatcg agatgaaaaa
     5401 ttaaaaactt ccctctacgt ccaatggatt ggatggtttc gccaacaaga gaagcctcaa
     5461 agatgggtgt ctctggttgc tcgatgatct tgggccacaa gacctgaact ttttgaatcg
     5521 tttttcctac tactaaccgt tccaatcctt tacgaacggt ttctacttct ggtaattctg
     5581 gcattttttc ttttcacctt cattatattg ctctgtctta aacaatgttt attttgcttc
     5641 gtaccacgtt tttccccaac tgctgtcagt gatcagtggt acatgaagag aaacagcttg
     5701 ttccataact tccttcacca atttatctaa tgattcaagt tctttttccg gtacctcgaa
     5761 gacaagttca tcatgaactt gtaacagcat cgtagcttga agacctgttt cttttaatcg
     5821 tttatccaat tcgatcatcg cgattttcaa aatatcagca gcacttcctt ggattggtgt
     5881 attgatagct gttcgttccg caaaagaacg gatattgtaa tttcttgaat tgatgtctgg
     5941 cagatatcga cggcggtgat acagcgtttc gacgtagcct tggtcttttg cttcgcgtac
     6001 gattctttcc atatattctt tgacacctgg gtatttttca aagtacgtat cgatgtattg
     6061 ctgtgctgct tttcggctga ttcctagatt ttgtgaaagt ccgtaatcag agatgccata
     6121 cacgatacca aaattcactg cttttgcctg cctacgcata tttggtgtga catcctctgc
     6181 cttttcgatt ccaaatacac gcatcgctgt gctggcatgg atatcttgac cttctaggaa
     6241 ggcagctttc aaatgttcat catttgagat atgcgcaagt acgcgtaact cgatttgtga
     6301 atagtcagaa gaataaatca gccagtcttt ttctctagga acaaatgcct gacgtatctt
     6361 tctcccttca tctaatcgaa tcgggatgtt ctgcaaatta ggatcaacag agcttaatcg
     6421 tcctgtttgg gtcaatgtct ggacataacg ggtatgcact tttccatctg attggatcac
     6481 tttcagcagt ccctcgacat aggtagattg aatttttgca atttgacgat aggtgaggat
     6541 atcctctacg attggtgctt gttcacgaag ctgttccagt acatctacgg ctgtggaata
     6601 tccagttttt gtttttttga taacgggtaa tcccattttt tcaaataaga tcactcctaa
     6661 ttgctttggt gaattcaaat tgaattcttc gccggcttct tcgtagattt tttgttcgat
     6721 ctctcttaag cgagcagaga actcgccctt catttcttgt aagcgagtcg catctacttt
     6781 gatcccgctt atttccatct ctgccaaaat gatagacaat ggcagttcca ttttccggaa
     6841 aagatcttct tggttttttt ctgccagttc ttggctcaat tttggtgtca acgcattgat
     6901 tgcagctact tttcgcgcta aatgagcaaa aaagagttcc tcttcttctg gtaaaccttt
     6961 tttcgcccct ttgccgtata cagcttcatc tgattggata tcgttgtatc cgtaatgagc
     7021 agctattcct tcgatatctg tgcttttttc attcgtatcg agaaggtatg cacctagcaa
     7081 gacatcaaaa tcgattcctt tcgtttttcc agtatagcgg tttaaagcaa cataagttcg
     7141 tttcgcatca tatacttttt tcagccgagt cggatcagtg atccaagaat ggaacgctcg
     7201 gttttcgaat acagatagat cgttcgtgac ataaattttc ttttctgtcc cccacgctac
     7261 tccgacgatt tcctctgtat gataattgtc gcccatcatt tccacgtaca acgccatatc
     7321 cgtagtaaac atttcctctt tgaattcatg aacgacttca aacaaaatat catccatttt
     7381 tacttcttct tctacgatat tcaatttaga aaggaactga ttgaaatcca tttctttata
     7441 aaacggaacg agtttatcga gatttttccc ttcatacttc aatgagtcga tagagacatc
     7501 cacaggcgct gaagtattga tggtcgccaa ttttttcgat aagaaagcct gctctttgtc
     7561 atttatcaga ttttctttca ttttgctttg cttcatttca tcgatatgtt cataaattcc
     7621 ttcgactgag ccatattgtt tcaatagctt gatagctgtt ttttcaccga ttttagtcac
     7681 accggggata ttgtcagagg catctccggc tagtcccttc atgtcaataa tctgttttgg
     7741 cgtcaaccca tcatattttt ccgctacatg ttctggtgta tagctttcga tatcgctgac
     7801 accttttaca gtgatatcca cttttacttc tttactagct aattgagtca aatcccgatc
     7861 acctgaaagc acgacgacat caaattgatc tttgtctact ttttctgcca aagtaccgat
     7921 aatatcatct gcttcataat tgggcaattc gtaatactgt acccctagtc ctgtcaacaa
     7981 atcacgtata tatggcattt gttctttgaa ttcgccaggt gttttggatc ttccaccttt
     8041 gtactccgca tacatttctg tccgaaaagt cgtttttcct gcatcgaacg caaccagaac
     8101 atgagtcagt tgctcttttt gcataacatt ttcaaacatc gtattgaatg catagattgc
     8161 atttgtatga agaccgtttc tatttttgaa acgttcaaga gaattgtgaa gcgcaaagaa
     8221 tgcgcgaaaa gccacactgt tcccatcaac gagtaataat ttattttttg tcatcttctt
     8281 aactccttac tgtaaagttc ttattcattc taacaaagaa tcctgcggaa tgcgaaaaga
     8341 aaaagcggga ccataccaaa taattcggta tagccccaac tgatgaattt ttttcttttt
     8401 tagttgttcc gtccaaagat tctcagaaac gccaagaaca agttgataaa gtccaaatac
     8461 agcttcaatg ccatgaatac agcaattcca ctatgtgctg ttccgtttgt ctggacatag
     8521 atattacgga tgctttggtt atcataagct gtaatccctg caaagatgac gactaacagt
     8581 agggagatga acagctcaac tacgctactt ctcaaaatga agatgttcaa gactagtgca
     8641 atgatcactc cgattaaagc tgcctgtcct gctcttccaa ccccgctaag atctcttttt
     8701 gttgttactc cgacaacaga catccccatg aacatcaaag tagctgtcaa gaaagcttgc
     8761 gttactgttc caatatcgta gtatgcaaga gtaaccgcta atgttatgcc attcaaaaga
     8821 gagtaggcaa taaatccacc gattgctaag ctcggattct tttgtgcttt tacgcctaga
     8881 accattacta aaatcagttc aacgatccac agacccacaa agcctagtgg aaaattattg
     8941 ataaaagttg ctaattgcaa aggaaatacc caaagagcca agtaagaagt gactgcacta
     9001 agggcaagtc ccatacctaa aaatccataa acttttgcat aaaaacgatt gatacctgac
     9061 attgatgtac ttggattatt cattcaattc accttcactt tcgattgcta tttccaattc
     9121 aggaacttgt ggcggttgga ccataacgac agcatccaac acacgatctt cgttcgtatt
     9181 ctccgcttct acagaaatcg tcaccacatc cttcatctta tctactttta tgacctcaaa
     9241 ctgaaaagtc agtgtttcat aatggaaaac cggttcaata aaattgacag aaaaattgac
     9301 aacatgtgat cctggtccag gaagatgctt tgaaattgca cttgtgatga tccccatcag
     9361 catcactgat ggaacgattg gctgttgata ttctgttttt tgtgcaaagt catgttggat
     9421 atacaaagga tttgcatcat tcgtcaatcc caaatacagc aaaagctggt tatcttcaat
     9481 agattctgtt aaggataacg agtcgccttc ctcgatttca tttattgttt ttcctagttt
     9541 tctcggctta cctatgttca agatagaaac cctccagttt atttttaatc atacattatt
     9601 gtaccaaaag tagcgtaaaa ggcaagttct gctcccttga tttaacagat tcctatagtt
     9661 tacagaggtc gtgaaaaagc tgtcctgcat caaataataa gaacagccaa gaaaaaacag
     9721 cttctcatgt ttttgatttg attgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     9781 ttatcataag ttgcaaaaaa gggggcttgg caagattttt gccactcccc ctctttctat
     9841 ttttataagg acactgcagt aattgtatta cagtacattt ttcgtcgtgc tgcttaaaag
     9901 tttttcatac gcttgttcaa atttttgaac atctccagca cccatgaaga ttactactgc
     9961 atcatgataa tctaacaatg gagatacatt attttctttg atgacttctc cacctttttt
    10021 gattttcgct ccaaggtctt caatcttaac gtttccttgt tcttcccgag ccgaaccaaa
    10081 aatatcgcat aagtaaactt tatcagctaa atcaagtgct tctgcaaatt catccatcaa
    10141 tgcaatcgta cgcgtaaacg tatggggttg gaaaactgca attatttctt tatctggata
    10201 tttttggcgt gcgccatcaa tcgttgcttt gatctctgca gggtgatgcg cataatcatc
    10261 gactacggtc atgtctgcta cgatcttttc actgaatcga cgttttactc ctgggaaagt
    10321 cagcatttct tctgcaactt ctttcagatc cagtttttcg aaataagcta ctgcaataac
    10381 acctaaagca tttaagatat tgtgtttacc gaatgctggt acagtgaagt gcccaacaaa
    10441 ttcatcccca tgatagacat caaaagcaga accagaagtt gtgcgttcga tgtttcgtgc
    10501 ttggatatca tcgttttctg tgacaccata ataataaatc ggcacatttg cttttaattt
    10561 gcgtaaataa gcatcgtcac catacgcaaa aatagctttt ttcacttgtc cagccattgt
    10621 ttggaaagct gtaaatacat cgtcgatact tgtataataa tccggatgat caaaatcgat
    10681 attggtcatg attgcatagt ctggtgaata ggcaaggaaa tgacggcgat attcacaggc
    10741 ctcaaatgaa aagaattccg cttgcggatc accatgtcct gttccatctc caattagata
    10801 acttgttgga cggacaccac tcaatacatg agagagcagt ccagtcgtac ttgtttttcc
    10861 gtgagagcct gttacagcga tactcgtata attttggata aaatgaccaa taaagtcgtg
    10921 atagcgaatc acttccaaac ctaattcttt tgctcgctgg atttcttcat gtgaatcagg
    10981 aaatgcattt cctgcaatga tcgtcatccc tggttttaca ttatctgcgt taaatggcag
    11041 aatcgtgata tttgcttttt ctaaatctct ttgtgtaaag aaatattttt caatgtctga
    11101 tccttggaca tttagacctt gttcatgtaa aacaagagcc aaggagctca ttcctgaacc
    11161 tttgatacca acaaaatgat acaatttatt ttgattttcc atttgaatcc tcttctctta
    11221 tctagcgtcc attcttttag ctattacatt caacacgctc ctcattatct tataaacgat
    11281 tgaaaatttc aaccaaattt atttggaaag gaggattcgc ttgctaaaat ggtcaagtac
    11341 gagaaaaatc cgtttttatt gactctttaa ttctggtgta agaagaccaa acttcctctg
    11401 atatatcaat gtgaggaata aaatcttttc ctttcggacc atagccgttc tcatcgtcat
    11461 ggagacgttc catttctcct aatactagcg catggaaata ttccttaggc cattgatgat
    11521 tcagttcttt tgctgtaaag atgatctctt cagaaaagtg aaggatttcc ggcacaaatg
    11581 gcacagcatt cgcgaaaata gtttgttcat tttgccatac tttctcaaaa gttgctcgag
    11641 tcctgcgttg cagtgtcgga tcattcatca ataagatttt ttctggtaca gctcctaagg
    11701 aatgaagaat ctccagtgaa aaagtggcgt tctctccaga atttgtagac ttcgattcta
    11761 tcagaaaagc cttatcgggc aaatcataga cttcttttag atactgacga cagatttcac
    11821 tttcactcat gccatcttca aaatgaaatc cttgcttttc aaaattctca taaaggattc
    11881 tagtcgcatg ccccacccct ccgaccaaaa acaattgatc tacttggcca tttaaataca
    11941 tttgtgctgc tttatcagct aagataggaa gacagtttcc agctaaaata agtgtaggtg
    12001 ccgtcagttc ctcaaaattt ccctcttcag gtgcggctaa aaaatttaat agctgattcc
    12061 agtcttttag attcctctgc tcactttttt ccaaaggcct tctatccttt catctgattt
    12121 tttgtgcttc attaaagtct tctctttttg tctcaatcca tttgatttct ccatgacatc
    12181 ttccacaacg atatttgcgt acatcgattc ttctcttgcg gcagatcgtg gcagaacaat
    12241 cttggcatcg ataatactcc atctttatct gtttcttcgt caaggctggt gcatagcgca
    12301 accctcctgt ttttttcaaa agatacttga aatccatatc tttatgctga taccctcttc
    12361 cagctagatg aagatgataa tggcaaagtt catgtttgat gattcctgca atcgtctctt
    12421 cgtctgactc ttcaaatagc ttcgcattaa aatctaaatg atgatcgttt aaatgatagc
    12481 gcccgccagt tgtacgtaag cgtggattga acattgcttg gtgcttgaaa ggaagctgga
    12541 agctttttaa tgatatctct tctactaact gctgcagttc ttcttgtgtc aatttttgtt
    12601 actcctcatt gttactcttc ataaggggaa agcatcgtca aactgattct gccttttttc
    12661 gtatcgactt gttctatcca aactgttacg atatctccta ctgacacgac atctgtagga
    12721 tgcttgacga attttttgct gagtttcgat atatggacaa gtccgtcttg cttcacaccg
    12781 atatcaacaa atgcgccgaa gtcaatgaca ttacggaccg ttccagtcaa ttccatccct
    12841 ggtttcaagt cttccatact caatacatct gtacgtaata agggcgctgg catctcatca
    12901 cgcatatctc gtccaggttg tgtcagccca gctaaaatat cggctaacgt ttcttctccg
    12961 actgataggc tttcagccag tttctctacg gataatcgtg tcaatttttc tactgcttct
    13021 tctgtaccaa gctctttttc tgaaagttgg actgaagtta atatttcttt tgcaatagaa
    13081 taactttcgg gatggatacc tgtgttgtcc aaaatgtttt tcccaccagg gactcgcaag
    13141 aatccgattg cttgttcata tgcttttggt cctaagcgtg gcactttttt caattgagtt
    13201 cgtgcggtga attctccgtt ttcttcacga taaatgacaa tgttttgggc agttgtttta
    13261 ttcaatcctg atatatgctg caagagctgc gggcttgctg tattcacatc cacacctact
    13321 tggttaaccg ctgtttccac aacaaaatcc aattgttcag ccaaacgttt ttgagaaacg
    13381 tcatgttggt actggcctac tccgacagct ttaggatcga ttttgactaa ttcagctaaa
    13441 ggatcttgca ggcgacgggc aatagaaaca gcacttcttt cttcaacttg tagatgcgga
    13501 aattcttttc gtgcaatttc gctagcggag taaacagaag cgcctgcttc gttgacgata
    13561 gcgtaatagg ttttatggtc ggcggatttc aactgctcag ccacaaaaag ttcagattcc
    13621 cgactggctg taccatttcc gatagctacc atatctactt gatatttatt aattaattgg
    13681 ataaaagctg gtccagcagc ttctcttttt gcttgcgctg caggtttatg aggataaata
    13741 acttcgattg ctaaaacttt ccctgtcgca tctacgactg ctagtttaca tccagtacga
    13801 taagccggat caaatcccag gacaacttta ccttttagtg gcggttgcaa caaaagatta
    13861 cggaggtttt cgccgaaaat agcgattgct tgctcatctg ccttttcagt caattcattg
    13921 cgcagttctc gctcgattgc tggctgaata aagcgtttat aactatcctg ataggcttct
    13981 ctgacgaatg aagaagaagg agaggcagga tttttgacta tttggcgatc caagtaagct
    14041 aaaattgctg cttcatctac ttgaaagaat actttcagaa tatcttcttt ttctccacga
    14101 ttagtagcta atattcgatg agaaaccatt ttatggacgg gttctgcaaa gtcataatac
    14161 atttcatata cgcctttttc atctgcttgc tcatccttga cattgctgac atacatacct
    14221 ttgttatacg tataagaccg aatccaagtc cgaaattttg cattatcgct gacttgttca
    14281 gcaataatct catgagcacc gtgcaacgct tcttcggcag aagagacttc tttttctttg
    14341 tcaatatatt tttctgcttc agattgcacc tctccatctg taagctgcat cagccacttc
    14401 gcgagtggtt caagtccttt ttctttagca atcgttgctt ttgtccgtcg tttttgtttg
    14461 tatggacgat acaaatcttc cacttgttgc attttcacgg cttgggtgat ttctgcttct
    14521 aactcgggag tcagtttccc ttgttcatcg attagccgaa tcacttcagt tttacgtttt
    14581 tctaagtttt caagataggc ataccgctct tcgatttcac gaatctggac ttcatcaaga
    14641 cttccagtca tttcttttcg gtaacgcgcg ataaaaggaa cagtgtttcc ttcatttaac
    14701 aaattaagta cagtagtcaa ctgcttagga cgatagtctg aaagttcttt ttgtacaagc
    14761 tgaatgatcg tctggttcaa attttccgtc attaaaatgc ctcttttctg ctaaaaattg
    14821 cttctttatt ctatcatact ttttatcctg ggttaaacta ctgaaagaaa caatattctc
    14881 tataaaaata aaaagagccg caacaaagtc acagctcctt cattctttcc attagtcaga
    14941 ttccatgcca aggtccttcg ttgttctgat attcaatatc ttgaagaata ccttccttgt
    15001 caaaaacaac cccatgcaat gaccctccga atacagcccc accgtccatt ccgattttat
    15061 gatcctgaat ccagaggtct gttgtctgca tatctccatg gagcattggt gtaatcgtat
    15121 gtccaaatac aattgtctta cctgtattgt ttttcccttc atgaaatggt tcccttatcc
    15181 aaataaagtc tcttagactt gtcttacgcc agtctttctt tgtcaaatcc acaccggcat
    15241 ggacaaaaag ataattttcc cattcataat acaaggggcg ctggactaaa aatttaatca
    15301 gttctgaata tctcgattgg atcatcatcg agatttcagt tggggagtat tcctccgatg
    15361 cccctgggtg aatcaggctt tcaaacgttt cttttccacc atttctcata taaggcgcat
    15421 accagtcctc cggtgattgc agaaattgca aaaaatattc ttcatggttt ccccggaggt
    15481 aaatggctcc tgtttcttcg actaattgct ttcctaataa aaaacattct tttgtacgag
    15541 gacctcgatc attgagatcc cctaataaaa caagctgatg gatctttgga tcataatagt
    15601 gaataagttt ttcaaaaagc tcatgtttgc catggacatc cccaatagca taaatcaatg
    15661 atttcatgtg gatcaccatc ctcaacgcta gtatagcgca tattggaaat ggtcgacaat
    15721 caaaactatt gttttctatt taattaaagc tgtaaaccgc agttctgctt cttctggaga
    15781 gattccatgg tcttgaacat agcgattgcg cggatgtttc gcacactcta tgctacatgc
    15841 tcctaaatgt ttttcttcat tttcaatgga agcaagaatc tgacgattgc actcaggatt
    15901 gctacaattg atatatcgtt cacatggtgt tccgtcaaac cagtcgcgac cgacgatttg
    15961 tttatctact tggttgatat cgacagcgat ccgttcatca aacacataca tcttgccgtc
    16021 ccataattct ccttggactt caggatcctt accataagta gcaataccgc catgcagctg
    16081 tccgacatct ttgaatcctt cacggacaag ccaaccagaa aacttctcgc aacgaatgcc
    16141 accagtacag taggtcacta cccgtttttc cataaattct tctttgtgct cgcggatcca
    16201 gtctggaagc tctcgaaaac tgcgtatatc tgggcgtaca gcccctcgaa aatgtcccaa
    16261 gtcatattca taatcattcc gtgcatcaat caccactgtc tgttcatcta aaatagcttc
    16321 tttaaattct tgcggtgaaa gataggcacc tgtcacttgg tttggatcaa tgtcctcttc
    16381 tagctttaaa cttacaagct ctggacggta tcttacaaac atcttcttaa aggcatgtcc
    16441 ttcgcttgga tcgatcttga acatcgtatc agcaaatcga ggatcagctt tcattgcttt
    16501 catataggca gtcgtgtttt caacggttcc agacacagta ccatttattc cttcttcagc
    16561 tactagaata cgacctttaa gatccaaaga tttgcaaaag gctaaatgat ttttggcaaa
    16621 ttcctctggt tcttcgattt ttgtataatt gtaataaagc agtacttgat aatccatgta
    16681 tttcttcctt ccttcttttc gttcctaaac agtataaaga aggcgttcgc atttgacgat
    16741 ttatttgctt gtgtataatt acgcatggaa agttttttgt ttacataata ttattatttg
    16801 aattaaaaag acttcgcttt cctgtttcgc acagaaaaac aaagtcttta tttctattat
    16861 ttacctgtaa ccgcaaagta gttgccatcg ggatcagcaa agttaaagac atattcttct
    16921 ccaagctgta ccatatcacc taatgtgact gattgcgtct gcattttttt atatagtcca
    16981 aagatatctt tactgtaaaa catcaaagat ggggcactag tagctgtgcc aggtgaatgg
    17041 gactcaacaa atgctcgatc atataacaga aatgaggttt gactgtcctc aactcctact
    17101 tcaattataa gcgtcccatc catttcttga cgatcttttt ccgcaaagcc aactgctctc
    17161 caaaacgttg catttttttc tacatcgtcc acataaagca tgatttttat ttcattcgta
    17221 aacatctgtt tcctcctaaa cgatctgttc taacattctc aactttgtca gtttaccata
    17281 ggaaaatggg aaaatctacg aaagctctca acagaatcgt cctacttaaa tacgcttttc
    17341 tttaataata aataagttgg ttgtttttgt tatttgcgct ttcacttttc ggaataaata
    17401 atgtgtattt atatggaata aataaaaaaa gaggtcgtga ctaaaaacaa gtcacaacct
    17461 ctggtaaacg gtgttctcct gacaactcct cggtcacaag acacaatatt caacaaacat
    17521 gagaagctat tttttgaaat tgctctttgt gactcagagt gaaacggcag gctcacgacc
    17581 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagctcaac caaacgtaaa
    17641 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    17701 aacctcttat tcacggtgtt tcagaagtag cttctcggaa ataagccgaa atttcacaaa
    17761 aatttgagaa actattttta ggaaacttct tcttatttct taaagctgac cacttctatc
    17821 acaacctctt tttgactatg cattcacatc gatcacttta gaatcatcat tgatgacatc
    17881 gcgatctaaa tctttgatgc gtgcacttgt cacgatacca gctaccatgc taccgctgac
    17941 gtttactgct gtacgtgcca tatcgattaa tggttcaaca gagataacta acccaacgat
    18001 tgctactggc aagttcattg cccctaaaac gatcagtgaa gcaaatgttg cgcccccacc
    18061 gactccagct acaccaaaag aactgattgt tacaattgcc acaagcatca aaataaattc
    18121 caaactaaag acattcactc cagctgctgg tgcgacgatc gttgccaaca tagctggata
    18181 aacgccagca cagccgtttt gtccaataga caaaccaaaa cttgctgaga aattagctgt
    18241 tgcatcatct actcctaaag cttttgtctg agtctcgata ttcaatggca aagctccagc
    18301 acttgaacgt gaagtaaacg caaagctcaa taccggaaag gcttttttga aatagttgat
    18361 tggattgact tttgcaccaa tcaaaatcag tgagtggaca atcaaaacag caagcaatgc
    18421 tacgtaagaa gcaacgacaa atttacctaa attgacgata gctgcaaaat cacttgttgc
    18481 caaaacgttt gtcatcaaag caaatactcc ataaggtgtc aaacgcaaaa caagcgtcac
    18541 gatccgcata atgattttat acagactgtc gactaaatct gaaaagaatg ctgcttcttt
    18601 tggcgctttt cttttcacgc ctagataagc gattccgaca aatgccgcga aaataacgac
    18661 tccaatcgta cttgttgcac gactacctgc taaatcagca aaaacgtttt tagggataaa
    18721 ggataaaatc tgctgtggaa tcgataagtt ttcaactgtt tcctgacgtg ttgccagttc
    18781 agagattcgt gctgtttctg cagctccttg tgtgaattta gctccttgga gtccgaacag
    18841 cattgttgaa ccaattccga tcaatgctgc aatagctgtt gttccaagta aagaagtcaa
    18901 tacagtcgca ctaattttcc ctagcttttc agaaactttc atttttgtga atgcaccaat
    18961 gatcgaaata aacactaaag gcataactag catttgcaag aatgatacat atccttgacc
    19021 tacaatcccg atccattcca ttgattgagt aacgactttg tcgtctacac caaagatcaa
    19081 ttgaagcaga ccgccaaaga cgataccaac tcctaatgct gtaaagacgc gtgttgaaaa
    19141 ttttgtatgg cgcttttgca tacgatagaa cacatataat aatgccgcaa aaactagaac
    19201 cactaaaact gtgatgagtg ttgtcatttt tctcctcctc atattattaa tgacaagtcc
    19261 atcgaaggaa gtaaaggttc ccgagaatga ttcttcaacc atcctcctga aaaaattttt
    19321 ttcagtgact tttatatttt agacgatttt tatcgaaatg acattctttt tgcttacaat
    19381 tagtaataaa aaaacatacc tgtgatagct ttaatctatc gcaggtatgt ttttactgtc
    19441 tattcttgtt tttctgattg aaaattcaat tttaccccag gctgatctaa ggcgatctct
    19501 gtcacctcat aagacattcg gcgtacgata tcgaataacg gtgctactag ctcgtccact
    19561 tcttcttgtg tgacatcatt ttgaccttca attttccgat tgaggatatg attgatctga
    19621 ctcacacttc cagataaaac atatgtatca aacactaaac ggaactctaa gcgtgctcct
    19681 acgatgctat gttctttcgg atagttttcg atcgtttctt ttaaaggcga gaagcctact
    19741 ttcaaatctg tttctacttc ttggttcggc aatctcatat cataatgaaa tgcttctaca
    19801 acttcttttt gtcttctgat ttccatataa ttttatcact cctttatgaa aattataaca
    19861 tatctccttt gaaaggggat actacttttt aatgatgctc tgcaaggtaa ggatttcatt
    19921 cagatttcct accacataat ctgccgggat ctcacccaga aaatgatcga tgtcgtaaag
    19981 acaagtagcg actcctgcat ttttccctgc ttcgatatct aaacgacgat cgccgatcat
    20041 catcgtgtct gttctttcta aatgaaaagt atcgatcaaa taatttaaag atgctggatc
    20101 aggtttacga ggaaagtcct gatcgatccc tactacttcc tcaatcagat gtgccaatcg
    20161 atgttctttt aacaggcccc atgttgattc tgtcaaacgg tgggtcagga tgaaatgacg
    20221 accgccgttg tcttttagca tttccagtac ttgtttcgtt tctggaaatg gacgggaaac
    20281 ttccgtactt tctttttcat atcggtgaaa taattcatta aactctctgg cattcaagtt
    20341 tgattcattg atcaaataac tagtagaata ctttttcatt ttgaagtaga tttctttttt
    20401 gtccatactg ataccaaaat cttttagtgc ttgccatgca ccatctacca ttgcgggata
    20461 cgtatcaaac aatgtaccat caaagtccca tatataattt ttcatgttcc ttttgttcca
    20521 ttcctttgct tcttttttct atctctcact tttagataaa ggtgttcttt aaagtatttt
    20581 tttgtccaga attgtcaaat catctgctaa tgtatactgg attggttgtg catttgggat
    20641 atcgatcgta tccatttgat cttcaggaac atcttctaaa tattttgtca gtgctcggat
    20701 gctattacca tgacccacaa ctagtaaatc ttgccgatct tttagttttg gtgctagatg
    20761 atcttgccat aagggaatga tccgattcac tgtcatttcc agactttctc cgtatgggat
    20821 caaatgcgga tccaagtgat catatctgcg gtcaaatcta ttttctgcgg ttaacggcgg
    20881 aacttccgta tatcctctgc gccatgtttt tacttgatct tttccgtata tttctttcat
    20941 cttatctttg tttttaccca caagatcccc ataatgccgt tcatttagac gccatgtttt
    21001 tattatagga agccataact gacccatttc ttctagtgcg acttgtccgg taagaatcgc
    21061 acgtttcaaa acagaggtat aaacggcatc gaattgaagt ccggcttcaa gcattttttg
    21121 accggcaact gctgcttgtt cttttccttt ttctgttaag gcgacatcta gccatcctgt
    21181 ccagtaattt tcaaaatttg cttcgctttc gccatgacgt aataatacta atttcattct
    21241 tttctccctt aaacattcgt ttcgaatttt ataccacatg ttttgcatgt cacacgtatt
    21301 cgtccttttc ctttaggtgc acgctgcttt tgatgacaat tcgggcactc aaaaaaagta
    21361 taactttttc gatccatttt gacttgcttt ttgtttttta tctgatgata tttttctttg
    21421 actctatcaa gatatttcaa ataacgttga ttctcattta gtctggggta gatttttttt
    21481 gataggaaac gaaatatcat ccatccgaaa aagacgatga acaacaattg tccgattcta
    21541 tagggcaacc aacgacttag caaactaaag acaagcgcag cgatcaatag accgcgattc
    21601 aactgatcaa atttcgcata tcttccctgc ataagtttgt gatagttctg ctgaaatcgt
    21661 attagccttt ctaaccatgt ttgcatcata ttatctcctc catcatctaa tatttattat
    21721 gacaaaaaaa tccaaaaaga aaaagcctta attggccttt tccattgcaa cttggtgaag
    21781 gacattccga tttctggtaa agtcaatcac aatcactttc tttttatctt ctctgttgac
    21841 tactcgaaat gtgtccgtca atggaagaaa cagcttctta gatggtagct gttgcatacg
    21901 tataaaacga tggatcatcg taatcaattg aggaaattga atagacgtgt gaagcagacc
    21961 tggatatttt ttcaacagct gtgtcgtttt gatcagattc cgttttttac tgatcacttt
    22021 taaaaagctt tttagaatct tttgctgacg tctaaaaata gaaaaagaat tttcgtcatc
    22081 tatttgataa cgcatttgtt tttgcagttc tgctgcagtc agatggatct tcccatcgtt
    22141 tgtatcgatc attaaacctt ctggaaacag ttcaaccact gctcgatccc aagattcaag
    22201 agaaagctcg atataatctt ttactggtaa ttccaattct accttaaaaa agtgcaaaac
    22261 agccgacagc ccttctgctt gatagatttc tcctaatgtt agcccctcta gatggaactc
    22321 tggtgaaaaa tgcagcaatg ttttattttc ctgcaattga acatatagaa atgtaaatcg
    22381 ctcaatcaga gtatctttta aaacaactaa aaaattcatt tttctccccc ttttttcatc
    22441 atttttaaaa tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa
    22501 atttactatt tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc
    22561 atccgccttc ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt
    22621 ctctatcgtt attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa
    22681 tcttagtgat accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa
    22741 tatcgatgtt gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta
    22801 ctaatgtcaa cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat
    22861 catgtacacc agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg
    22921 aaaagtcgat aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat
    22981 tcaatgctac aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat
    23041 tctgtctttt tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg
    23101 tgtcaatgca atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat
    23161 ttgtgcagcg attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc
    23221 tactccaccg ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat
    23281 aaatgctagc ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg
    23341 ccacatagac aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt
    23401 cgatcggatt ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg
    23461 ctcagcaaac cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta
    23521 aggcaatccc gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac
    23581 tccgtgcagg aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa
    23641 gaaacgcttt ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat
    23701 aggtgccact gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg
    23761 ccagaaactc attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa
    23821 gcaataccga ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc
    23881 atccctccgg caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc
    23941 gaatcaaaag caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct
    24001 atgtagccta tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca
    24061 aaggctagaa atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc
    24121 tctccgtcag atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc
    24181 gtgatcacga tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg
    24241 atcatatatt tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa
    24301 aagagcgtca ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct
    24361 ccttctaacg gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata
    24421 gcagcaatca cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt
    24481 accttaaaat gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca
    24541 acataaaata acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa
    24601 cgcgcttatt gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg
    24661 caaaacaaaa aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat
    24721 tccatagtct ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt
    24781 atcaggagat tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt
    24841 gatgattgca gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat
    24901 acttagttct tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt
    24961 aacattttgt cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac
    25021 caagaaactg ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg
    25081 gatcatcaat gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc
    25141 ttttcgttta ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc
    25201 aaacacagtg gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag
    25261 ctcattggaa aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc
    25321 cgatgtgata tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat
    25381 ggaaataaat gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg
    25441 aagttctaat gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt
    25501 tttcatcgac tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa
    25561 acaataacaa aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa
    25621 ttatagatgt attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt
    25681 tgtacttcat caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg
    25741 attggtttta aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca
    25801 atagatgctg cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc
    25861 atattgtatc cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt
    25921 ggatgagtca accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt
    25981 acaaggccag attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg
    26041 atacatggaa gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt
    26101 aaaaggttgg aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac
    26161 aggaatgtat taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta
    26221 cctaacccgt taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc
    26281 aaaaccatcg gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca
    26341 tgcccgtttt tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt
    26401 atctacatga aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt
    26461 ttcgtttgtc tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa
    26521 ttgtgcttct acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat
    26581 gattcgatga agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt
    26641 cacttctggt atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta
    26701 aaattcagaa gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag
    26761 tccgatttct ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag
    26821 gaaatctttc gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc
    26881 aaggttagaa gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt
    26941 ccattttcca gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg
    27001 gctagaagct actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg
    27061 ttcgactaga tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc
    27121 attgtcttgg atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg
    27181 cataatcatc cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt
    27241 cttttctttg acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc
    27301 ctcttctgta taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc
    27361 cgaatcgaaa ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg
    27421 tgcaaaatga tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt
    27481 caaatagcct tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt
    27541 cccgctcgct aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac
    27601 ttctatctct gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt
    27661 gaatgtctca tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc
    27721 acttccgcat cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa
    27781 ctttctgatg ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac
    27841 attcatcata ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct
    27901 cctaaaataa taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga
    27961 ccaaagatcg aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga
    28021 aataagtatt tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg
    28081 taattcttat cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt
    28141 ctgagcacgg gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt
    28201 ctttggtaca tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa
    28261 tcgaattatc gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta
    28321 gctatgatga atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt
    28381 taaagcctcg attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa
    28441 tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc
    28501 tttcacagca gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca
    28561 ccttgttttt ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca
    28621 aaaacagtcg atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt
    28681 ttctaccttt tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag
    28741 gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga
    28801 ttcttttgca atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg
    28861 acaacgtttc tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt
    28921 aaccaaccac gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc
    28981 tttagctgtc aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc
    29041 agcttatttg aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga
    29101 cgagcatgtt gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc
    29161 cgatgctgag tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac
    29221 ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt
    29281 cgtccaggaa aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt
    29341 ttcatatagc tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata
    29401 tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt
    29461 gacggaaatc tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa
    29521 agaagagaca ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa
    29581 aaaatcaaag agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt
    29641 ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag
    29701 ctgttcaatc aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg
    29761 aatgatacaa tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat
    29821 ttttccaatg atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa
    29881 atgaatgact ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga
    29941 gaaaatggaa gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt
    30001 tttcaatctg cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt
    30061 gcacaccacg gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa
    30121 ctgaaaaata cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa
    30181 ctagcgaatc tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac
    30241 gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt
    30301 ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt
    30361 cctctgtacc gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac
    30421 caaatgggcg tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc
    30481 tcattggtat ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg
    30541 ctaaaacggc tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa
    30601 gtccaattgc tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa
    30661 cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag
    30721 atacagcccc attttattta taatacgctg actagcttga aatttttgat ttggcagaaa
    30781 aaaacagatg aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt
    30841 gggaaaaaag aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc
    30901 gatcttttga gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa
    30961 ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt
    31021 catgcttttc acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag
    31081 cttcttttat tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa
    31141 aaaaatgatc atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg
    31201 tatggcgatg catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg
    31261 atttgtctac cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag
    31321 cgctgatttt tttattttaa gttaaataaa tcaatcagta ttcttgactt tcatctacgc
    31381 gtctcttcta ctgtcagcac tggataacag cccaatattt gactttctcc tccgagcatc
    31441 ttgatttctt ggagcgcatt ctctactaat gtcattggac ggtccaacaa caaatcgatc
    31501 acgaaaaaat attctcccaa gctagttttc aatggtcggg attcaatctt acttaagttg
    31561 atttcccgcc agccaaaagc tgccagcatt ttatgaagca tccctggccg attagcaggt
    31621 agtgtcaaga tgacactcat ttttactgga gctgactgct ggctggtcat ctttcggtca
    31681 ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt ggatattctc agctaagatc
    31741 tccaatccaa catgctgtgc tgtttctaat gaagcaatcg ctgctgcgtc ttctttcgga
    31801 tgttctgcta catacattgc tgcagcagtt gttgattctg ttgctactaa aggaacatta
    31861 ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg cttgtggatg tgaaaggatt
    31921 tttgtaatct ttgttgctgg attgcctaaa agctgctgct tgataggaag aacgatttca
    31981 cttttaactt cgacttctgg atgtttggaa agaagatcga ttgtgtgatg aacagctcct
    32041 tccaatgaat tctctacagg aacgacagcc agatcgactt gtttgcgaaa caaagcgcgc
    32101 aaacaaacag ggatggaagc atacgcagtc aattgttctg taggaaatag ctgacaagcc
    32161 gcctgataag tgaatgaact ttctggccct agatagctga cgttcatatg cttttgatcc
    32221 tttctataat ggtatcgatg atcttttctg gtgtttgatt ggtcgtgttg attcgatgtg
    32281 tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa tgcttttatt tcttctcttg
    32341 tttttccaag agctaaaggt cgaatagacg tttcatcttg ttggatacga tcaactagac
    32401 agtccgcttc tgcctctaag taaagaacaa tcggctgtgt ttttaaaaaa cgccgatttt
    32461 ctgcactttg gacaatccct ccaccagtcg cgataatttt gtcttcattt gacagctttc
    32521 ttaaaatatc tgactccact tttcgaaagc ttttttcacc gtaatagtga aaatattcgg
    32581 caatcgacaa ttggatatgt tcctcaatct ttgtatctaa atctacatac tctttttgta
    32641 aacattgagc taacccttta ccgattgttg tttttccagc gcccatgaaa ccaatcagca
    32701 aaatgctagc catgagcttc tcctcctgac acaagttctg ctaaatcgtc aaaaaatgcc
    32761 ggataagaga ttgaaacagc ttcagctcct tctagttcgc aaggtccatc agctatcaaa
    32821 gcagctactt gcagcatcat accgattcga tgatcgcctc tgctggacac ttttgcgcca
    32881 tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg tcgcttttat ctttgctccc
    32941 attttctgta attcttctgc caccgcatca attctgttgg tttcttttac ttttaattct
    33001 tctgcgtctt tgatgatcgt gattccttct gcttgagtag ccactaaagc tagaatgggc
    33061 aattcatcaa tcagacgcgg aatgatttct ccaccaatcg ttgctttctt caaatgactc
    33121 gtcttgacac ttaaatcagc cgattggttc tgttcattgt gattcgtctg ggtaatcgct
    33181 gcgcccatct cctccaacac atctaagata ccagttcttg ttggattgac cccgacattt
    33241 tttaacaaca gttggctttc cggaacaaga agtcctgctg ctagaaagaa agcagctgat
    33301 gaaatatcac ctggaacagt tatctgttga ccagttaatt tttgcggtcc ggtcactatg
    33361 atcgttttat cttccactgt gatccttccg ccaaattgac gaatcatttc ttccgtatgg
    33421 ttacgggaac gttctttttc aacgattacg gatgtgcctt ctgcttgtaa tgccgcaaac
    33481 agcaaagcag atttgacttg cgcactggct acaggcatat tgtatcggat aggtgataaa
    33541 gagcgggttc ctgtcacagt gattggtaga tactggtcat tttcttctcc ttgcaagtca
    33601 gcgcccatct cgcgtaaagg ttccatcact cgccccattg gacgtttaga caacgaagca
    33661 tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa tacccatcaa taaacgtatg
    33721 gtcgtaccgg agtttcccat gtcaattgat tcggttggtt ggacaaatga gtggctaccg
    33781 cgaccatgaa tcttgatcac ctcttcctct tcttcgatct cgacacctaa ttgttggaag
    33841 gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat tttgtatagt cgtcgttcct
    33901 tcagcaattg ctccaaacat gatgctgcga tgggaaattg atttatctgc tggtatccta
    33961 acagtccctc ttaatccatg tatttgttgt aataattgca tttatccagc ctcctatttg
    34021 atccagcaat gataactggt attagacata atgattcttt ttgcttgtaa caaatcctgt
    34081 tggtttttaa aactgatttg cagtattcct aaaatatctt ccctggtctc ttggatcttc
    34141 aaattaatca aagaaactcc cgcttttccg atcagtccag ttacttctgc aataacgcca
    34201 ggttcatctg gcacatcgat aaataaatca aaaaaggcag gaatcgcgcc ttgcttatgg
    34261 acaggcaact ggtctcgact ttcttttgcc cgattgaaaa aagaaaagat ttccgattgg
    34321 ttctcagtca tgatccattc tgctatttgt gtcatttctt gttgccaatc tgccaatcgt
    34381 tccaataata tctgcttatt gctcaacaaa atatctgtcc acatgacagg atctgaggaa
    34441 gcgatcctag tgatatctcg aaagcctcct gctgccagtt gtttggctcg cggatactct
    34501 tgattgaatt gttctgcctg attgactaag ccagctgcca gaatatgtgg aaaatgactt
    34561 agcattcctg ttatacggtc atgttctctt gctgtaagag ttacatattt tgctttggtt
    34621 ccgcaaaata gttcttgcag tttttcaaca tcttttttcg tcttttccaa aggtgtaaag
    34681 atgtaatagg cattttcaaa tagtttagca tctgctgcct gtatacctga tttatgagaa
    34741 ccagccattg gatgtccacc gacaaaccga agatttttct gctcagcgga tgtcacgact
    34801 tgctgcttcg tactgctgac atccgtaatg atgacgtgat cagacaagtt gattctttca
    34861 aggacatcaa gataagctaa ggttgtacgg acgggaacgg ctaataagat aacgtctgct
    34921 tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa cgatctgctg ttctactgct
    34981 tgctcacggg tttttgactg actatcccaa ccaactacta tcagttctgg attggtctga
    35041 cgaatacaaa gtgccataga agcaccaatc agtccaagcc ccacgataaa aacatgttgt
    35101 gccatcttat ttgccccctt tttagtaatg tttcaagtag tcacggtact ccttcactgc
    35161 agctttcaac tcaacaagcg aatcacttgg gaattttgac aatagctctg tcgctaccac
    35221 agtggctaca acattttccg caacgacgct agctgcagga actgctgtac tatccgaacg
    35281 ttctacactt gctttgtaag gttcttttgt atcgatatca acactttgaa gtggcttgta
    35341 caatgtagga atgggtttca tgaccccgcg aatgacaagt ggttcgccat tggtcattcc
    35401 gccttcgaag ccgcctagat tattggtacg tcggaaatag cctctttctt cttcccatag
    35461 gatctcatcc atcacttctg agcctggtaa tgaaccagaa gtgaatccga ccccaaattc
    35521 tacgccttta aaggcattga tactaaccac agcttgtgcg attttggcat ctagcttcgt
    35581 gtcccattgt acgtagcttc ctaatccagc tggaacgcct ccaacgagca cttctacaat
    35641 tcctccgatc gtatctccat ttttctttgt ctgatcgatc aaatctttta ttctttgctc
    35701 aactgctgga tcaacaactc gtacttctga cgcttcagaa atttctttga tttcttgaat
    35761 cgagatattt ccaggtattt ctgcttggat tccaccaaga caaatcacgt gtcctgctac
    35821 gatgatgtca agttcttcca gaattttctt ggcaattgcg ccgattgcta cacgcatggt
    35881 cgtctctctt gcagaggacc gttcaagcac attccgaaga tctcgatgat ggtatttcat
    35941 accgcctacc aaatccgcgt gtcctggacg cggacgtgca actcgccgca gtgattggtc
    36001 tttttcaggc acttctgcta cagacatgac agttgtccaa tttttccaat ctttattttc
    36061 taccactaaa gtgacaggtg aaccaattgt tttgccgtga cggatgccag acgtgatccg
    36121 aacacgatct ttttcgatct tcatccttcc tcctctgccg taaccagctt gtctgcgagc
    36181 gagttcgtga ttgatttctt cttctgacag aggtaatcct gctggtagtc cttcaataat
    36241 ggctgttaat tctggtccat gggattctcc cgcagtcata taacgcatct tacagtcctc
    36301 ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat ttttgcttgc ccaatctttt
    36361 ctagcaagat gatattcaat tggcttcctc ttgctttctt atcatgggtt atcgcgtgga
    36421 ataattcatc tggatgactt agatcgtacg taatcggcag atggaatttt tgaagtatcc
    36481 tttctaaagc agctgtcgat ccagttggag acaagccgat tttttctgca tgagaagtga
    36541 tggctaccat tccaatagct actccttctc catgactgac taccccatat cctgctgtat
    36601 tttcaattgc atggccaatt gtatgaccaa aatttaaaat aagtcgattt ccttggtcaa
    36661 agacatcttc ttccactact tttcgtttga tttccaacgt agcagtaatg attggaacag
    36721 catgttccag aagatctttt tcgtctttca gctcgttcag ctgtttccaa agcgttgcat
    36781 ctgctatagc tgctgatttg atgatttcag cgattccttc tcgtaaacga cgtaccggca
    36841 aagtttttag aacctcagga tcaatcaata caccagctgg ctggctaaat gttccgacaa
    36901 ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc aatactagaa tctacttgtg
    36961 caagtagcgt agtcggaatt tgaacgaaat ggatcccacg catgaacgtc gaggccacga
    37021 atgctgctag atcacccacc attcctccac caagtgcaag tatcccatcg ctgcgggtaa
    37081 aatcattatc tgctaaaaaa tcgtataact cagctgcttg ttctaaagat ttactggtct
    37141 cacccgccgg tacgattgaa aggactggtt caaaattagc ttgtctcaat tggaacatga
    37201 ttttttctgc atatagtgga gcaacgtttg tatcagtgat cacagctact ttttgaggtc
    37261 tccaaatcgt ttgaagccat gatccagcct gttctagtgc gcctttttca atatgaatat
    37321 cgtatttttg gttatcaaca tgtacagtaa gcattcgttt cacttctttc ttttaaaaag
    37381 ctggaattaa tcgagtgtct gaatcttttc cattgcttct ttcatgatcg caacttcttt
    37441 catcatgcgt gagtaagtct tttcattcaa cgattgggga ccgtctgacc aagcatttgc
    37501 aggatcagga tggatctcta cgatcatgcc atctgctccg cttgccacac ccgcacgagc
    37561 cattggtggt acaagttccc aaattcctgt accatgactt ggatcaacaa tcacagggaa
    37621 atgagttaat tttttcatca atgggacagc acttaaatca aacgtattac gtgtcgctgt
    37681 ttcgtaagtc cgaatcccac gttcgataaa aatgacagga gatttgtctt cgactgcaat
    37741 gtattctgca gcattaagcc attcattgat cgtaccggaa attccacgtt tcaatccaac
    37801 aggtttacct gttttaccga cagcagatag cagtttaaaa ttttgcatat tacgtgcgcc
    37861 aatctgtagg atatcgctgt actcagccac catatcgata tgcccttcat ccatcacttc
    37921 agtaatcact ttcattccaa attcatcagc cgcttgacgg atgtatttca atccttcttc
    37981 ttctaaacct tggaaagcat atggagaagt acgtggtttg aaggcaccac ctcgcagaat
    38041 ttttgcgccc ccggctttgg ccattcgcgc acattctctg atttggtcta atccttcgat
    38101 agaacaagga ccagccatcg tcacgaaact gccgtcacca atttttactc cgtctacatc
    38161 cacgactgta ttgccaggat ggaattctcg agaagtcaat ttgtatgtgt ttaaaatacg
    38221 cacagcattt tctactcctt catagcgtgt aaatgctcct tcttgtaaac ttcgcgtatc
    38281 accttttagt ccaatcacta ctcgttctgt tccttgatcg attgagactt tgaatccttc
    38341 tttttcgata tgatcgacga ctttttttat ttgttcttcc gttgcttctt ttttcataat
    38401 tactatcatt gtttttcccg ctttcttttg tttacttatc tatcttggct gattttctct
    38461 actttttcaa ggtggtttta cattaattcc tactaaatga ccgtgtcctg ttgttctcat
    38521 tttcgctcct tctttcctac ttatgaccca gataatcaac aaaaaaagat gtccctgtag
    38581 gaaattgcct ctacagtgaa catcttcaac gagccttaaa aagcgctgca aaaatataaa
    38641 atagccacta tagatgaatt tctctacgtg gctgtaagtc gtgtttaaaa atctttagcc
    38701 atcatagata caaacctggt ttgtttgtac ccacgaagtc atgaatacaa acctatctaa
    38761 aatagctaaa gtaatacgta ttcagttttg taagttttct agatttcata aaaaatcgct
    38821 ccaaactgat gtaagaattg tacctagtat agacataaaa aaatcgactg tcaatcattt
    38881 ttatgaaact ttttttaatt ttctgatagt taactttgtt tttcagaaaa ataacaattg
    38941 gttttattga agaaaatagt catgaacaaa agtatctgag aactttttta ctctcttgtt
    39001 catgacatat agaataaagc agctttgtct tgtaaactcc gtttttcaaa taataatcta
    39061 aagattgcta ctgaataata aagccgattt catggtcctc ttcgctgctg attcggtaag
    39121 gtgactcaac atccgctccc cagctattga tacccccgac tcctcttact tttgcaaaaa
    39181 tcgtaagaac cgttcgtctt tctggcggta gttcttctat atgtgttgcc tgttccagtt
    39241 cttctgcttt gaatggtaaa gcggaaaaag caaatggcgt atcctgctgt tcaaatcgca
    39301 acgaaaaatc ttcttgatct ttatccgtat tatccaacgt ttgatttctt gttagttcta
    39361 cccatttcgt ttccatatgc atgccgcatt cttggggaac aaggtatggg gaaacgggga
    39421 gtcctgccac tttataaata ccttcgacac caccagccat ccgatccgga tatgtttccc
    39481 ctgataggcc ttcatactca aaaaattttg ctttcgttgg cataatgaac cttaaaccca
    39541 atgcgggtaa ttccggtaat cctttttgtc cataataatg catattgaca agcattttac
    39601 cctctccagc gatttggtag gaaattgtca cttgggtttt tggtactgtg ttgatttcca
    39661 gtgtaaaaat gatttcaacc gtttcagcgt attcattact tgaatattga ttatttactg
    39721 gagcaatcgg caaatcgatc tttttattgt ccaccagtac ttctatttct ttgacttttg
    39781 gatacaatcc ggctcctaac cacataccgg atttaaatcc aaatccgcta cctctgtcat
    39841 tatcggtcgt tgctcgccaa aaaactggaa gtgtttcccg gtatagccat tcttttccgt
    39901 ttttattgag cgattccagt ccgcctcttg tataagaaaa aatataatgc gtattccccg
    39961 atttgacacc taatgtacag tcgccgaaga cgattgcaac tttttccgta tccatatgac
    40021 ttttatttac tgtatttttc ataataatat cttacctcct gcatggcggg tttttcttgt
    40081 ccatctgcaa aaacgatccc attccctgag aattcatagt cacttggttt atcgtcaaag
    40141 tcaccgccat agcgcatcac ttctctgcct gtgattggat cagtcacttg gatcgcctga
    40201 tctttaaaat cccatatata gcctccttgg tacatttcat aacgatcaag caacgtcatg
    40261 taagaatcca ttccacctaa cgaattcccc atgtcatgca tatattcaca taagatgaac
    40321 ggtttttccg ctttattttc aaggtagtca atgatctctt ctggtggcgc atacatgcag
    40381 ctttttacat ctgaaatcga atcttcgaat gctggatttg gaaaattccc ttcataatgg
    40441 acaagtcgat cggggtctac ttttttgaag taatcgttca tttgtcgaat gttttcttcc
    40501 gcataagatt catttccaag agaccaaaaa agaatacttg gatggttttt taatgtttcg
    40561 aaattcgttt ttgctcgatc aagaacagcc gcctgccact gaggtactgc acctggtacg
    40621 ttgtaagaag gctcaaatgc gcccattttt tgccatgagc catgggattc gaggttcgtt
    40681 tccgccatca catggattcc tgcttggtcg cataaataat accaagtgat ctggttgggg
    40741 taatgagatg tccgtacgcc gttgatcttt gcatttttta tgatagccaa atctttttcc
    40801 atatcttcta tagtgatggc gcgtcctttt ttcggatgcc actcatgtcg atttaccccg
    40861 ttgataatca tccgttttcc attgaagtag atgattttat ctttgatttc gattttacga
    40921 aaaccgaagg aatagtcaat atactccgtt actctttttt cttcgtctat cgtctccaat
    40981 tcaagcttat ataaattggg ctgaaaatgc ccccacagtg ccacattttc aaatccatcg
    41041 atgcgaatcg tcatttgctc ttttaacgga tattgctgtt gatagatttc cttcccatca
    41101 ggatctagta tcgctacatt cagctgactg tttttggcta cttgctcaaa agacaaatct
    41161 acttcaagtt tgccattttg acatttgtca tctaagcgag gtttgatcca aagatcttct
    41221 aaatggtttt gtcctcgagc aactaagaag acatctctga agatcccaaa aaatcggaaa
    41281 aagtcctgat cttccaagaa tgcagctgta cttcttttgt aaacctccac agctagaata
    41341 ttttcttctt ccaaataagg tgttaaatca aattcagacg gagtgaacga atcttcggca
    41401 tacccgataa aatgaccatt caaccaaata aacattgctt gttctacacc agcaaaatga
    41461 atcgagattt gttttccctt tagaccttct tctaatcgaa aagtcgtcaa atacgaacca
    41521 accggattgt ctttagcagc tgaaaagatc ccttccgcct ctttctcagg gcttgtcgtg
    41581 aaagggggac ggcgaaatac atgtccctcc cacggataca tcgtattgat gtaatggatc
    41641 ttgtcataat ttgctaattc aatatgacta ggaacagtga tttcatcaaa agaacaggca
    41701 tcaaaatctt ttttataaaa tgtttttggt cgtaccgaag gatttggcga ataacaaaaa
    41761 cgccatgatc catttaaaga ctgtttaagg gaggatgtgg tttctacttc ttcccttgtt
    41821 gcataacaaa catgatcgct atgtgcattt gattggttca ctctaaaaac ttgtggatca
    41881 tctaaccatt ttatatttgc tttcatactt ttttccttct ctctcataac tttttgaaat
    41941 gaagcagttc tgtttggaaa tcaccttgaa aatcggggaa gtaaagtcca actgtcatca
    42001 attcggcccc cgaaaatatc cgcttcgagg tctgttcctg ataaagtgcc tcatcatcta
    42061 accccatcag atatacttca tggaacgctg gttgagctga agctagtatc ctccctaaaa
    42121 acacaatagc ttcatcttgt ttaggagaca caaacatcca tgcggcaata ttttcttcaa
    42181 aaggactcgc caaacgataa taatggccat actggatcac cggacgaatc gtttgatagg
    42241 atataatttg gtcttttact tgcttttttt cttcaggaga cagctttgtc aaatccagtt
    42301 cataaccaaa aacagcgctc atagctgttt ctcctcttgt tttcaacgaa gtagatctgt
    42361 cggtctgatg atttggtaca gctgatacat gagctgtgat ggacgaaatc ggataagcaa
    42421 ggcttgttcc atattggatt ttcatacgct ccactgcatc cgtgttatca cttgtccatg
    42481 actgcggcat ataataaata aacccggcat caaaccgtcc acctccacca gaacagcctt
    42541 cccataagat ttccggataa tcagttgtca aatgttcaag tagttcataa agcccgagca
    42601 tataacgatg cgctacttct ccttgtctct gtgggtcaag ggtaatcgaa tagacatctg
    42661 aaagcgaacg gttcatatcc catttgatgt agtcaagagg aatcgtatct aaaatctttc
    42721 tcatttgttt ctcaatcgtc tgccgaacat ctaaccttgt aaaatccagt acatgttgtg
    42781 atctggaagc agaaggcatt ctacctggct cctgcataag aaaatcagga tgtgttcgat
    42841 acagttcaga atcaaccgag atcatttcag gttcaaacca taaaccaaat ttcaatcctt
    42901 tgctatgagc ataatctgct atttcacgta atccatttgt caacttgcct tcatactcga
    42961 accagtctcc taatgaagaa ttgtccgcat ctctttttcc aaaccagcca tcatctaata
    43021 cgaacatttc gatgcctagc tcctttgctt catcaatgat cgccttgatt ttttcactat
    43081 tgaagtcaaa gtaagtggct tcccagttat tgaccagaat cggccgttca gcaaattgat
    43141 gacgacctct tgcgattctt tcacgtagca actcatggtg gatctgactc attttgttca
    43201 atccctgact tgagtaagaa aggatcattt caggtgtttg aaaagactgg cctgcattca
    43261 attcccacga aaaacgatgg ctgttgatcc caccgacgac tctcaattga ttgatctgat
    43321 ctttttcaag ttcgaaagaa tggttgccgg aataaacaaa ttgaagtccg atcgcttctc
    43381 cagtattttc tgtcgtatga tgatgaacta aagcaataaa gttattcata tggtgactgc
    43441 tcgttcctcg acggctttca aatacttttg ttccagataa aacagattgc cgtgaaatct
    43501 gtctttcatt gacatgtgct cctggcaaag caatgacttc atcaaatcgc ctagtatggg
    43561 caaagtctag ctggaaacta gccgcttttt ctagtttgat tgtttctccg gtttcatttc
    43621 ttatctttac agatcgtgtg atcactgctc tatcagtaaa tatggtatag aacaaatcga
    43681 aataaatcgc tgtttctcta tccttaagtg aaatcaccaa cgtttgtgcc tctgacttgt
    43741 ccatcacata agtttgaggc aagccttcga tctctggctt cccatctgta atataatggc
    43801 tgtcatatct cagatccagt aagtctgatc cattcttgcg ctgaatgatc gatgcgggcg
    43861 ttcgaaagtc catgctgcca tgagaagagt attcttgcgg tagtgtatct ttcgacagag
    43921 accggtcagg atttaaggga acatttcctg aaaatccacg atctcttcgg ggataatttt
    43981 tattgtcttt gtactgcttg actggtttgc caaaataaat atgcgccaat acattgcttt
    44041 cttctatctt tatcagataa gaaagaaatg tatttcgtaa atggaagact cggctctttt
    44101 catcaaataa gatgaaattt ttatctgttt ttctatgcgt aatatccatc atttcactcc
    44161 caacaaaacg gaaaccgttt actttttttt ataattcctg tatactgttt actaaacaat
    44221 tttgtcaata aaaatttttt atttttttga ggagtgtgta aaaatggttg gaattagaga
    44281 tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc cgtgtgttga ataacgatca
    44341 gacgatccat gtggctcaga ctactcggca acggatagta gaggcagctg ctgcgttgga
    44401 ttatgacatc agtactcgta aatatatcaa aaagagaatg ccttcgattg gtgtgatctc
    44461 tacgatcggc aaagcgagcg aagaacagga tatctattac aaacatttgc gagtagggat
    44521 ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga atctataatc tatctgataa
    44581 tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg atcgttgttg ggactgttac
    44641 gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa atagtcgtcg tggataaccc
    44701 tgacatccag caagaggtcg atatggtgta tgcagatctt gaacggatga ctaaaaccgt
    44761 actggaattg ttcttagaaa atggtcataa aagaatagcc tatatcgggg gatataaagt
    44821 cgatatggat gaattcggtg ggaagcagat ttctataaat gaaaagagga tgcgtgccta
    44881 tcagcatttt atgatcgatc atcacttaga aaaatatatg tcttatcatc taggtggctg
    44941 gacagaaaaa caaggtgagg tacttgcgac taatctatta gaaaatgaat cccctcttcc
    45001 tactgccatt cttgtcggga gcgatccaat ggcaattggt gtctatcggg cattgaaaca
    45061 aaaaaatatc caaattggag aagagattgt tgtagcaagc tttgataata tcgagacaat
    45121 cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat gcgaaatcaa ttgggcaagc
    45181 agctgttcga atggcattgg agcgaattga cggtatacgg gacgaaccaa tcattttgac
    45241 ttatcctgca cgattgattg ttagggaaag ttttatccca agaacaaaaa ggtattgaaa
    45301 gagaggttgt gaaaagccgc ttcgtttctt catactcaaa acgaaacggc tttttcacaa
    45361 cctcttactt atacttattc acaaaatcca atgtctgctg gtaacaattt tgcgattctt
    45421 ttagcgtgta atcaaattgg tattcatggg taatctcttt ttttgtatct gtataaaaca
    45481 aggattgcac gggtaccttt aattctgtca accgattttc taatgcttga ccttgctcct
    45541 ggaatgagta tgcatttccg tcagttatat acgttggcgg aaattcctta gaaacatggt
    45601 cagccacact agcttctttc aattgctcac tcgttttcca atcttttgtt ccaatcaacg
    45661 accacgcaac ggttttgacg aaaaacttca taaatcgatc atccgaatgc tgctgtgctg
    45721 ttagcttcaa atcaacaggt ccgcaataag aaatggttcc tttcagacta tcttttggaa
    45781 gtagctgttt gattttcatt tgtttagcat acgcatcatt cgtttgtata gctgcatatt
    45841 gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa taattgttcc atgtctagca
    45901 atttgtcgtc tttttcttca gctaataaat ctttgatcag ttcattcact tgaagaactt
    45961 gattagggta ttctgaatca ggcgctaatt cataattcat cgcaaccact gcgatgttgg
    46021 cgtctgaaac taattttgtc gcaaattctt tgacacccga tttatctccg ccgacgaatc
    46081 ctccaccatg tacccaaaat agaacaggaa caggcccctt gctgtttttt ggatagtaga
    46141 tgtcataagt attctcttta tacttggact catacgtcaa atctgttttt ttcgccacgt
    46201 ttttttctgc ttgatcgaac gtttttttat cagtgatttg aacagtattt gaaaacatat
    46261 ggttgatcaa gaatgctcca ggtctggggg aaagctggaa ggcaagaaag attgcaaacg
    46321 cgatcccaag aatgattccg aagaaccatc cgataaattt aagaattttc tttttcataa
    46381 ggctccctca gtagttagta ttaagcattc tgattatacc atagtgttat ttcctatcaa
    46441 gctctcctag atgatttaat gattttcccc aaacttcttc tgcaatgctt ttgacaaaag
    46501 aaattttttc cattgttctt caaacaaaac attttcttct tctgttgaag caaatccaca
    46561 ttgaggactt aagcacaaac gatccaaagg cagatatcgt gtcgcttcgt agatccggtc
    46621 aatcacttgc tggtgctctt ccatttcgcc atttttcgtt acagtcaaaa tctaagacaa
    46681 ctaatttatc ttcagagact ttctctaaaa gaacaaaact cccagcacgt tcactatcgt
    46741 attctaaata ataggcacgg acattttctt gagtaaaata atgggtctgc tacgtcgtca
    46801 tcccccctga tgccgccaaa atcgaataat aatttccgcg tcaaacatgt atatttagtg
    46861 ttgaatcatt tgagaatatg aaaataaaaa tctttttgat caatcagtta atcaaattga
    46921 aataacgaaa aatctgagac agaatgacgt tcattctgac ccagatttga ttttgcacaa
    46981 ctcaacagtc cttattggca aatcgccaaa ataatgtaga ctaactaaaa agactaagaa
    47041 acaataacaa aagttaacac ttcttagtct ttctttagca cgtctattta ttaaagattt
    47101 tagggccttt tctcatgttt ttacgatcat tcttaggtgt ttccatttgt ttggatcggc
    47161 ctccaccttg tttctttttg atcaattctt tcattttttc tttttggtcc atttttattc
    47221 tcctttaacg aatgaagcga ctaattcgat atgatgggtt tgcgggaaaa gatctacagg
    47281 ctgaactttc ttcacggcat aacctgcttc tgcaaataac ttagcatctc ttgcaaaagt
    47341 tgccggatta catgagatat agacgattcg ttctggtgac atttcgattg ctgattcaat
    47401 aaaatgatga tctaatcctt ttcgtggagg gtcaactacg atcacagatg gtttgatccc
    47461 tttttcttgc cattttttca taactttttc tgcttttccg acttcataat gaacattttc
    47521 gattttatta tttaaagcat tgaactgtgc atcttcaatt gcttctggaa taacttccat
    47581 cccatacacc ttatccactc tgtctgctaa agacaagcca atcgtaccta ttcctgaata
    47641 agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac tcgatggctg tttggtacag
    47701 atactctgct tgttgagtat ttacttgata aaatgacttt gctgagattc gataggtttt
    47761 tcctaatagc tgatcttcga tatagtctct gccatataaa actttttctt tgtctcctaa
    47821 aatcacattt gttttttctt cattgatatt ttggatgacc gaaaccactt ctggcaattc
    47881 ttcatgaata gcttcagcaa tttccttgga tttaaagaat ttttctttac gagtaactaa
    47941 aacgaccatc atttcatggg aataatgacc acgacggata atgatgtgac gaagctgtcc
    48001 ttcatttttt tcttcattat atcctcgtac ttggaaccgt tgcaagatat ctctgactac
    48061 tttgattgct tgatcgatga caggatcttg gatataaaaa tcttcgatag ggactagatc
    48121 atggctattc tttttgtaaa aaccggtggc taactgtcca tcgatacggc gaacagggat
    48181 ctgtgcttta ttacggtagc cgaatggttc tgccattccg accgttggaa gtacaggaac
    48241 ttctggcatc ttagcaatct tcgccattac atgctctact tgtttttgtt tgaagacaag
    48301 ctgttgttca tacttcaaat gggctaaagg tgcgatacct atgcgaagta atgcattgtt
    48361 ttctacaggc tgacgatcag gtgattgtgt aataaatgtt tcgacttttc catatccaaa
    48421 cttattgcct gcttttacta ctagtatttc tacttcttct ccagggagtg cattttcgat
    48481 aaataatgga taaccatcga tttttgctac ccccattcct tcataactca agtcgacgat
    48541 ttcgactgta tatcgttcgt ttttctttac tatttgctgt tctgtcatat tttgctcctt
    48601 taagcctatt tttcaatcct actgaactct actataaatc agctaattac acaactaaaa
    48661 ccggcactga ccttactacg ttcagcaccg gcttttgatt aattcttttg attaggtgtt
    48721 tcgtctgcta tttttccgtc accgtcgata tcttcatccg ttaaacgttc gacttctttt
    48781 acgaattctt tgctgacaac ctctaattct tcttcatctg tacctgtgat tgcttcatca
    48841 ggaatctgat cggtattggc aaagaactca atgtgttgat gaagattttg gaaatgcatc
    48901 ggtgcttccc cgccgtattc accatctaag ttgatcatta ctggtcgatt gatatcatct
    48961 tctgtttcaa cgaacaaact gcttgttttg gtatagatta ttcttggatc atcgacatgt
    49021 cgaccgccat tcagcatgag tgctgcaata tgcaagatct caaagatatt tgctgtcttc
    49081 acaatgatta aagaaaactt cccatcatcc aatttggcat ctggcgcgat ctgttcgaat
    49141 cctccaacag agttcgtcag ccctaggaaa aacatcgaag catttccgat atattcccct
    49201 tcatcatact tcaagcgcat tttgattggc tttactcgag ggagcatctc tgctcctttc
    49261 gcaagataag caagataacc aaaaatactc tttagttctg atggaacttc ataagtcaat
    49321 tctgtcaaat agccacctgc agcgatattg atgaagtaac tgttatcaga aaggccgatg
    49381 tccattttta cggtttggtg tttttttatc acttctgctg ctgctttaat attatctcta
    49441 ggaattttta atgctcgagc atagtcgttc gtcgtaccag ctgggatgat ggccatttta
    49501 ggacggtgtt caagcggagc gattccatta accacttcgt tgattgtgcc atcgccacca
    49561 gctgccacta ctagatcaaa acctagttct gcgactctac atgcttcatt cttggctgaa
    49621 tctggttctg gtgtagttgc ataggcgctg gcttcaaatc ctgcttcttc taatgcctgt
    49681 aggatatccg ccaaattttt tttcaataat tcttttcccg aggtggggtt ataaattacg
    49741 cgtgcttttt tcattttcag cctctatcgt ttcgctaatt catctttaag tagcttattc
    49801 acgacaccag gatttgcttg tcctttagtt gctttcatga tttgaccgac taggaagcca
    49861 accgcacgat cttttccatt tttgaaatca tcgacagatt gttggttgtt atccaaaatt
    49921 tcattaatga ttggcagtag tttgttcgga tcagataatt gcacccaacc atttttttca
    49981 actacttctt gggcttctcc gccatttgtg atcaattcac ggaagacttt tttggcgatt
    50041 tttgagctga tcgttccatc ggcgatcaaa cgaatcattc ctgctaagtt ctcaggtgtc
    50101 aattttgtct cagctaattc cagtttctca ctgttcaagt aagcagaaac ttcacccatc
    50161 aaccagttag atgcttgctt ggcatctgca ccattttcca aaacagcttc aaaaaaatca
    50221 gacatttctt ttgtaagtgt caataccatt gcgtcatact ctggaagtcc aaactcacgg
    50281 atatagcgtt cgcgacgaga agctggcatc tcaggcaaac tagcttttac tttttgaatc
    50341 cattcgtcat cgatcacgaa tttcgggata tcaggctctg ggaagtaacg ataatcacta
    50401 gacccttctt tgacacgcat caaaactgtt ttattcgttg tttcgtcaaa acggcgtgtt
    50461 tcttgttgga tttctcctcc tgaaagaaga acttttgctt gacgtttttc ttcaaaagct
    50521 aaaccttttt tcacgaaaga taaagagttc aagtttttca attctgcttt cgtaccgaac
    50581 tcttcttgtc cataagggcg aagagaaata ttggcatcac agcgcataga gccttcttcc
    50641 attttcacat cgctgacttc tgtaaattgg ataattgatc gcaatgcttc tagataagcg
    50701 tatgcttctt ctggtgaacg catatcagct tcagaaacga tttcgatcaa aggcgtgcct
    50761 tgtcggttca aatcaacgta agaataaccg ccaataccat gcatgttttt tccagcatct
    50821 tcttctaaat ggacacgttc gatccggatt tttttctttt taccttctac ttcgatttcg
    50881 atccatccgt catgaccgat tggttgatca aattgggaaa tttgatatgc ttttggatta
    50941 tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt tagagatttc acagtttaat
    51001 gccaaagctg ctttcatccc gaattccagc gcagctttat tcattactgg cagcacgcct
    51061 ggataacccc agtcgatcac gtttgtattg ctgtttggtt ctgcaccaaa atgagctggc
    51121 gctggagaga aaatttttga gttggttttt aactcgacgt ggacttctag tccaatgact
    51181 gtttcaaaat tcattcgtct ttccctccta aaatagctgg ttgttgttta tggaattctg
    51241 ttgcttgttc aaacgcatat gccgcttgat acatcgtttt ttcatcaaaa taattaccga
    51301 tgatctgcaa tccaactggt aatccttctg ataaacctgc cggtacagac atacctggaa
    51361 gtccagccaa gttgacaggg atcgttaaga tgtcactcat gtacattgtg attggatcgt
    51421 taatgttctc accgattccg aatgcaactg ttggtgaaga tggcccgatg atcaagtcat
    51481 aatctgcaaa gactttttca aaatctcgtt tgatcaatgt acggacttgt cctgcttttt
    51541 tgaagtaagc gtcataataa cccgcgctta aggagaatgt acctaacatg atacggcgtt
    51601 ttacttcttc accgaatcct tctgaacgtg agttgacata tacatcatct aatgatttca
    51661 cattttcaga gcgataaccg tagcggatac catcgaatcg ttgcaaattg gaacttgctt
    51721 ctgatgaggc aatgatataa taaacagcca caccgtattt tgaatgaggc aagctgactt
    51781 cttccacaac agcacctaat gaacggaatg tttctgctgc tttcaataca gcttctttta
    51841 cgccaggggc cacaccttcg cctaagtatt ctttaggtaa agcaatcttc attcctttga
    51901 tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac gcctgaagaa gtaccgtctt
    51961 tttcgtcata accgctgata gctgtcaatg ctaatgcatt atctttcacc gtacgggtca
    52021 ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa accaaaacgt gagacacggc
    52081 cataagtagg tttcatccct acgatgccgt taaaagctgc tggttggcgg atacttcccc
    52141 cagtatcact acctaaagaa actggaattt gtccagctgc aactgctgct gcagaaccac
    52201 cagaagagcc accaggtact tttgttgggt cccaagcatt ttttgttttc ttgaaataag
    52261 aagtttctgt acttccaccc atagcaaatt catccatgtt tagcttacca acagggatca
    52321 tatctgcttg atagatttta tccatgaccg ttgcatcata aattggttca aaattgtaaa
    52381 ggatcttaga tgcagcggtt gtcaagatat cttttgttac gatattatcc ttgataccaa
    52441 ttggaatacc agcaaggacg ttgctttcat caatcccttt ttcatctaaa gcttttgcct
    52501 gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc gacctcaggc tctgtttctt
    52561 tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt gatttctttc gatacaagca
    52621 tatgatgcag ttcttctaga gaacgattgt gtaattctgt cattatgcgc cagcctcccc
    52681 attatcgatg attgcgggta ctttgataaa gccgtcttct gtttccggca catttttcaa
    52741 taattcatcg cggttccatc cagcttgcgc ccgatcttgt cgcatcacgt ttactgtttc
    52801 aaccacattc gacgtaaatg gaacaccttc cgtatctact tcttccagca tttcaaccat
    52861 atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt tcttcatctg cgaacgcaag
    52921 ttttgctaat ttggcaacgt gtttcacttg ttcttcacta atcgccatac taccccttct
    52981 ttctaaacga gttaaaaact ttgctctcta ttttaacaca agagtgccgt gtctgaaatt
    53041 gtttattgaa atttgtctga aaaaactcat ttttcattct tctactgagc gacttttagc
    53101 caatgcatct gccatttgat cttctgtcca aacttcgatt cctaattctt gtgctttggt
    53161 cagcttgctg ccagcatctt ctcctgctac cacgatatcc gtttttttcg atacgctacc
    53221 agtcactttc ccgccaagat tttcgatcgt ctcttttgct tcttcacgag taaatcgtgt
    53281 tagtttacca gtaaggacta ctgttttttc tttaaaagga gactcgactt cttgcaattg
    53341 ggcatttcga agccctttgt attcgaaatt gacgcctgct tgttttaatt cattcatcaa
    53401 ttcatggact tcttcgttac tgaaataagt gactacactg tcggcaatga tatcaccgat
    53461 cgtatcaagc gcaataatcg actcatagtc acttttactc aatgtttcta gatcaccgaa
    53521 atgttctgct aaaatttttg ctgctttcgc tccgacatgt cggattccta acccgaaaat
    53581 caaccgctcg accgaattat ctttgctgtt gtcgattgct gtcaaaatat tatttgctga
    53641 tttttctttt attttgtcca acgtcaataa ctcttcttct gtcaatttgt aaagatcagc
    53701 aacatctgct actagttttt tatcatacat ctgttctagt acgcgcggcc ccaacccatc
    53761 gatattcatc gcatttcttg aaacaaagtg gttcaaacct tctttcattt gagcagggca
    53821 tttaggattg atgcaccgta aggctacttc ttcatccaaa tggacaagtt cacttccaca
    53881 aactggacaa tgtgtaggca attggtattc ttcgctgtct ttaggtcgtt tatctaacac
    53941 gacttgcgaa acttctggga tgatgtctcc cgctttataa atcagaactg tatctttcaa
    54001 gcggatatcc ttcaacttaa tataatcacc gttatgcagg ctggcacggc ttactgtcgt
    54061 tcctgctact cggactggtg tcatgatagc tgttggcgtt actacccctg ttcgaccgac
    54121 tgtccattcg atgttttcga ttagcgtttc aacttcttct ggcggaaact tgtaagctgc
    54181 tgcccatctc ggtgctttga tagtaaaacc aagttgatct tgtagcgaga attcattcac
    54241 tttgatgacg attccatcga tttcataggg caaatccaca cgtttgtcat gatattcttc
    54301 aatgtatgac cacacttcat caatcgaatg acatagacgt ttttcccgat tcgtgtggaa
    54361 tccaattttt tctagctctt ctagcgcatc atactgggtt tttgcttgca taggaccgaa
    54421 atcagctact gtgtataaaa aagtgtccag atttcttttc gctgtgattt tagaatctaa
    54481 ctgacgcaaa gaaccagctg cggcgttccg aggattcgca aagatatctt ttccctctgc
    54541 ttctctgtct tggttcaatt ggacaaatga ccgttttggc atgaaacatt cgcctcttac
    54601 ttcgatgttc attggttctt ttaatttgat tgggaccgac cggacagttt ttaggttttc
    54661 agtgatgttt tctcctacag ttccatcccc gcgagtcgca cctctcacga aatttccatc
    54721 ttcataacgc aaagaaacgg aaagtccatc tatttttagt tcacagcaat agtcaactac
    54781 acgtcccact gctttttgta cacgttgatc aaaagctatc agctcttctt tgctgaaaac
    54841 gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt tcaaaacctt ctaatacttt
    54901 tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa tcaggatatt gttcctcgat
    54961 tgctactaat tctgcgtatt ctttatcata aacatagtct tccactgttg gtttatcttc
    55021 tacgtagtat tcgcgagacc attggttcag tcttgtacga agttcttctg ctcgtttagc
    55081 agcttcgtca atcgtcattt catgttccat catttaccct ccactactaa gctttttcaa
    55141 ttggcgcaaa ggcagccaaa agacgtttta ctccttgact tgggaaagcc acatctagtt
    55201 ctagatcctt cgacgtcccg cttactcgga cgactgttcc tagaccccat tttttgtgtt
    55261 tgactttatc tccaggtttc cagctgttat tttcccctcc agtagcactt ttagagggaa
    55321 caaccgctgt ctctggctgt ttatagctat atgctgtttt tttagcgaat acaggcgttt
    55381 ttttaggcgt aggacgcatg ccttcgattt ccagtaactc ctgatcgatt tcttctacaa
    55441 aacgagaagg tcggttatat tgcgtccgtc catataatgt tctggagaaa gcatttgtta
    55501 aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc gagacggcgt tcttcctcca
    55561 gttcgctttc ttccatcaat gctcgagata aaggaaaaac cccctcttct agaccaatca
    55621 ggaatacaac tggaaattct aatcctttgg ctgcatgcaa agtcatcaat gtcacttggg
    55681 aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc cagatcattt aagaagaccg
    55741 ttagtttttc ttctggtgca tcagcgtctt cttcattttg ctgttcgaat tgtttgtcga
    55801 attcttgtgt gaccgttaaa aattcctcca agttttcgat gcgtgcttgt gcttctaatg
    55861 tattttggat ttttaagtct tctagatatc cgctacgatc caaaacctct ttcgtcaatt
    55921 ctgtcactgt caagtatggg atcatctgag tgacttcttg gatcatctct ccaaatgcac
    55981 ctaattgctg tcctgctttt ccaccgatat ttgccagatc cacattttgc gctgcttcaa
    56041 gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt ttcgatagat cctggtccga
    56101 tgcctcgttt gggcgaatta acgatacgct caaaactgat tgaatcttga ggatttacca
    56161 gcacattcaa atacgctaaa atatctttga tttctttacg atcgtagaac ttgtgccctc
    56221 caaccatttt ataaggaata tttgctttca acagagtttc ttccattaca cgagattgtg
    56281 cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg atgattgctt cgaatctctt
    56341 cttgcatccg gtctacgata aaacgggttt cgtctcgttc attatctgca cggtagtaag
    56401 tgatcttgtt tccttctttg ttttctgtcc acaagttttt tggttttcga ttgctattgt
    56461 tttcaatgac ttggtttgct gcgctaagga tgtttttcgt ggagcgatag ttttgttcta
    56521 gcaaaattac tgcagcatct ggataatctt tttcaaaatc caagatgttc tgcatatctg
    56581 caccgcgcca gccgtaaata ctttgatctg catctcccac gacgcacagg ttcctaaatc
    56641 tacctgccaa taaattgact agcgtatact gtgcgtgatt tgtatcttgg tactcatcta
    56701 catgaatata atggaatttg ttttgataat acgtgagtga atcaggatgt tcttcaaata
    56761 atcgaatcgt attcatgatc aggtcgtcaa agtccataca ttgattatat tgaagctctt
    56821 tttggtaagc agcataacat tttgccgcaa tctcttcgta ataactgcct tgcatttttg
    56881 caaaatcctg aggggtctgc aggctgtttt ttgcttgaga gattgttcct aatatacttc
    56941 gaggatcata ttttttcgga tcaatgttca gttcttttag tatccgtttc atcaaggtca
    57001 gctgttcaga agaatcgatg attgtaaagt tccggttata cccgataaaa tcaacatctc
    57061 ttcttaaaat acgcacacac atcgagtgaa aagtcgatac ccagacatct tcgcctccag
    57121 atgcgagaat cgcattgaca cgttctttca tttcttttgc agccttattg gtaaaagtaa
    57181 tcgctaggat gttccaagga ttgacttctt tttcctcaat caaataggca atgcggtgtg
    57241 tcagcactct cgtctttcca cttcctgcac ctgccattag cagtaatggt ccgtcagtgt
    57301 gaagaacagc ttctttctga cgggggttca tcccgtttaa taattcagct ttggatgaca
    57361 ttaacaacat cctttctttt atcaatcttg ttaattatag cattttcttg gctttcatta
    57421 aactagctag ttcaaaatca tttgtttctt tttatggatt tttccttgtc aattcgttga
    57481 tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga
    57541 aattagataa gactcagata tattttattc taaaaggaat atttgttgga ctgctgacag
    57601 gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct aagcgattta gttcgaagta
    57661 tctatgaaat gagtcgggaa cagccgattt acttaattgg cctagctatt tgctgcatcc
    57721 ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc ggatatcaag ggaagcggga
    57781 ttccacaagt agagggacag cttcgcgggg aattatcaat gaactggttc tctgttttat
    57841 ggaaaaagtt cattggcggt gtactttcag ttggtgcggg cctttttcta ggtagagaag
    57901 gaccttctat acagcttggc gcatccattg gacaaggagc tggacaattg tttcggagtc
    57961 catcttcaga agaaaagatt ttaatttcta gcggtgcaag tagcggactt gcagcagcat
    58021 tcaatgctcc tatcgcagga ttactctttg ttttagaaga aatccatcac tccttttctc
    58081 cacttgtttg gctgacatca tttgcttcag caatcactgc taacttcgtc tctctttact
    58141 ttttcggctt gagacctgtt ttatatctag gtgaccttcc ttctttgccg ttgaaatatt
    58201 atggttcctt agtgatctta ggagtacttc taggcatctt aggttttatt tatcagaatg
    58261 tcttgctggc tttgccaaaa ttttacaacc gattgccttt accagcttat tttcacgggt
    58321 tcgttccttt cttgctgatt ttgccaattg gttatttatg gcctagtact ctaggcggag
    58381 gcaatcaaat cgttttagct tttggacatg cttccttgcc aatcattatg attatcggtt
    58441 tgtttgttct tcgattcgtt ttttccatga tttcttacgg tgggggtctt ccagggggga
    58501 ttttccttcc gatattgaca ttgggggctt taatcggtag cttttatgga aacattatgg
    58561 ttgatttcgg gatggatcca atctacatca aggattttgt cgttattgcc atggctggtt
    58621 atttcacagc gattggaaaa gcaccgctga cagcaattat tttagtgaca gaaatggttg
    58681 gctctcttaa tcatctgatg cctttaggat tggctgctct tgttgcttat atcgtcaatg
    58741 accttctcgg cggtaatcca atttacgaat cattattgga acgtctactt ggaaatactc
    58801 agaccgcctc actaaaaggt gcaaggacaa cttttgattt tccagtaacg gcagagagtt
    58861 ctttagacgg attaatggta cgagacttca attggcctaa aaatatgctg ctaatctcta
    58921 tacgcagagg aaatcaggaa atcttgactc acggtgatac gatcatgaat gtaggagata
    58981 ttttggttat cttgactgat gaagcaaact tgccaagaat caagcaggaa atcgaggcta
    59041 aatcttctat cacaaatttg aagcaatcat tttaacgaac atggaaaaac tattttacaa
    59101 caactattca tctacactac ttgattctat atgattctat aaaaaaagaa gaggctggga
    59161 aaa
//
LOCUS       DASJRQ010000006        51483 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJRQ010000006 DASJRQ010000000
VERSION     DASJRQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.006"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            210..581
                     /locus_tag="U4A80_000320"
     CDS             210..581
                     /locus_tag="U4A80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1973991.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(855..1547)
                     /locus_tag="U4A80_000321"
     CDS             complement(855..1547)
                     /locus_tag="U4A80_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1973992.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1550..2335)
                     /locus_tag="U4A80_000322"
     CDS             complement(1550..2335)
                     /locus_tag="U4A80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1973993.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2572..3972
                     /gene="lacG"
                     /locus_tag="U4A80_000323"
     CDS             2572..3972
                     /gene="lacG"
                     /locus_tag="U4A80_000323"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1973994.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3997..4314
                     /locus_tag="U4A80_000324"
     CDS             3997..4314
                     /locus_tag="U4A80_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1973995.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4327..6000
                     /locus_tag="U4A80_000325"
     CDS             4327..6000
                     /locus_tag="U4A80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1973996.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6014..7645
                     /locus_tag="U4A80_000326"
     CDS             6014..7645
                     /locus_tag="U4A80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1973997.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8136..9701)
                     /gene="guaA"
                     /locus_tag="U4A80_000327"
     CDS             complement(8136..9701)
                     /gene="guaA"
                     /locus_tag="U4A80_000327"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1973998.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9760..10683)
                     /locus_tag="U4A80_000328"
     CDS             complement(9760..10683)
                     /locus_tag="U4A80_000328"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1973999.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10879..11484
                     /locus_tag="U4A80_000329"
     CDS             10879..11484
                     /locus_tag="U4A80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1974000.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11792..12808
                     /locus_tag="U4A80_000330"
     CDS             11792..12808
                     /locus_tag="U4A80_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1974001.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12949..14250
                     /locus_tag="U4A80_000331"
     CDS             12949..14250
                     /locus_tag="U4A80_000331"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1974002.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14296..14958
                     /gene="deoC"
                     /locus_tag="U4A80_000332"
     CDS             14296..14958
                     /gene="deoC"
                     /locus_tag="U4A80_000332"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1974003.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14979..15377
                     /locus_tag="U4A80_000333"
     CDS             14979..15377
                     /locus_tag="U4A80_000333"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1974004.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15443..16525
                     /locus_tag="U4A80_000334"
     CDS             15443..16525
                     /locus_tag="U4A80_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974005.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16737..18299
                     /locus_tag="U4A80_000335"
     CDS             16737..18299
                     /locus_tag="U4A80_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974006.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18292..19425
                     /locus_tag="U4A80_000336"
     CDS             18292..19425
                     /locus_tag="U4A80_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974007.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19425..20381
                     /locus_tag="U4A80_000337"
     CDS             19425..20381
                     /locus_tag="U4A80_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974008.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20480..21646
                     /gene="deoB"
                     /locus_tag="U4A80_000338"
     CDS             20480..21646
                     /gene="deoB"
                     /locus_tag="U4A80_000338"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1974009.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21670..22488
                     /locus_tag="U4A80_000339"
     CDS             21670..22488
                     /locus_tag="U4A80_000339"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1974010.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22505..23212
                     /gene="deoD"
                     /locus_tag="U4A80_000340"
     CDS             22505..23212
                     /gene="deoD"
                     /locus_tag="U4A80_000340"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1974011.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23510..24196)
                     /gene="gpmA"
                     /locus_tag="U4A80_000341"
     CDS             complement(23510..24196)
                     /gene="gpmA"
                     /locus_tag="U4A80_000341"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1974012.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24368..24949)
                     /locus_tag="U4A80_000342"
     CDS             complement(24368..24949)
                     /locus_tag="U4A80_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1974013.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25082..25765)
                     /gene="rpiA"
                     /locus_tag="U4A80_000343"
     CDS             complement(25082..25765)
                     /gene="rpiA"
                     /locus_tag="U4A80_000343"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1974014.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26084..26497
                     /gene="rpsL"
                     /locus_tag="U4A80_000344"
     CDS             26084..26497
                     /gene="rpsL"
                     /locus_tag="U4A80_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1974015.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26581..27051
                     /gene="rpsG"
                     /locus_tag="U4A80_000345"
     CDS             26581..27051
                     /gene="rpsG"
                     /locus_tag="U4A80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1974016.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27145..29232
                     /gene="fusA"
                     /locus_tag="U4A80_000346"
     CDS             27145..29232
                     /gene="fusA"
                     /locus_tag="U4A80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1974017.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29404..30591
                     /gene="tuf"
                     /locus_tag="U4A80_000347"
     CDS             29404..30591
                     /gene="tuf"
                     /locus_tag="U4A80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1974018.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30998..31306
                     /gene="rpsJ"
                     /locus_tag="U4A80_000348"
     CDS             30998..31306
                     /gene="rpsJ"
                     /locus_tag="U4A80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1974019.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31337..31966
                     /gene="rplC"
                     /locus_tag="U4A80_000349"
     CDS             31337..31966
                     /gene="rplC"
                     /locus_tag="U4A80_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1974020.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31994..32617
                     /gene="rplD"
                     /locus_tag="U4A80_000350"
     CDS             31994..32617
                     /gene="rplD"
                     /locus_tag="U4A80_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1974021.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32617..32907
                     /gene="rplW"
                     /locus_tag="U4A80_000351"
     CDS             32617..32907
                     /gene="rplW"
                     /locus_tag="U4A80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1974022.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32946..33779
                     /gene="rplB"
                     /locus_tag="U4A80_000352"
     CDS             32946..33779
                     /gene="rplB"
                     /locus_tag="U4A80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1974023.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33822..34100
                     /gene="rpsS"
                     /locus_tag="U4A80_000353"
     CDS             33822..34100
                     /gene="rpsS"
                     /locus_tag="U4A80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1974024.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34123..34470
                     /gene="rplV"
                     /locus_tag="U4A80_000354"
     CDS             34123..34470
                     /gene="rplV"
                     /locus_tag="U4A80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1974025.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34484..35140
                     /gene="rpsC"
                     /locus_tag="U4A80_000355"
     CDS             34484..35140
                     /gene="rpsC"
                     /locus_tag="U4A80_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1974026.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35143..35577
                     /gene="rplP"
                     /locus_tag="U4A80_000356"
     CDS             35143..35577
                     /gene="rplP"
                     /locus_tag="U4A80_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1974027.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35567..35755
                     /gene="rpmC"
                     /locus_tag="U4A80_000357"
     CDS             35567..35755
                     /gene="rpmC"
                     /locus_tag="U4A80_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1974028.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35780..36046
                     /gene="rpsQ"
                     /locus_tag="U4A80_000358"
     CDS             35780..36046
                     /gene="rpsQ"
                     /locus_tag="U4A80_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1974029.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36104..36472
                     /gene="rplN"
                     /locus_tag="U4A80_000359"
     CDS             36104..36472
                     /gene="rplN"
                     /locus_tag="U4A80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1974030.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36509..36817
                     /gene="rplX"
                     /locus_tag="U4A80_000360"
     CDS             36509..36817
                     /gene="rplX"
                     /locus_tag="U4A80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1974031.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36845..37384
                     /gene="rplE"
                     /locus_tag="U4A80_000361"
     CDS             36845..37384
                     /gene="rplE"
                     /locus_tag="U4A80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1974032.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37403..37588
                     /locus_tag="U4A80_000362"
     CDS             37403..37588
                     /locus_tag="U4A80_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1974033.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37624..38022
                     /gene="rpsH"
                     /locus_tag="U4A80_000363"
     CDS             37624..38022
                     /gene="rpsH"
                     /locus_tag="U4A80_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1974034.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38054..38590
                     /gene="rplF"
                     /locus_tag="U4A80_000364"
     CDS             38054..38590
                     /gene="rplF"
                     /locus_tag="U4A80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1974035.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38751..39107
                     /gene="rplR"
                     /locus_tag="U4A80_000365"
     CDS             38751..39107
                     /gene="rplR"
                     /locus_tag="U4A80_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1974036.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39128..39628
                     /gene="rpsE"
                     /locus_tag="U4A80_000366"
     CDS             39128..39628
                     /gene="rpsE"
                     /locus_tag="U4A80_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1974037.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39643..39822
                     /gene="rpmD"
                     /locus_tag="U4A80_000367"
     CDS             39643..39822
                     /gene="rpmD"
                     /locus_tag="U4A80_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1974038.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39866..40306
                     /gene="rplO"
                     /locus_tag="U4A80_000368"
     CDS             39866..40306
                     /gene="rplO"
                     /locus_tag="U4A80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1974039.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40306..41601
                     /gene="secY"
                     /locus_tag="U4A80_000369"
     CDS             40306..41601
                     /gene="secY"
                     /locus_tag="U4A80_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1974040.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41663..42310
                     /locus_tag="U4A80_000370"
     CDS             41663..42310
                     /locus_tag="U4A80_000370"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1974041.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42501..42719
                     /gene="infA"
                     /locus_tag="U4A80_000371"
     CDS             42501..42719
                     /gene="infA"
                     /locus_tag="U4A80_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1974042.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42752..42868
                     /gene="rpmJ"
                     /locus_tag="U4A80_000372"
     CDS             42752..42868
                     /gene="rpmJ"
                     /locus_tag="U4A80_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1974043.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42886..43251
                     /gene="rpsM"
                     /locus_tag="U4A80_000373"
     CDS             42886..43251
                     /gene="rpsM"
                     /locus_tag="U4A80_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1974044.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43279..43668
                     /gene="rpsK"
                     /locus_tag="U4A80_000374"
     CDS             43279..43668
                     /gene="rpsK"
                     /locus_tag="U4A80_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1974045.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43748..44686
                     /locus_tag="U4A80_000375"
     CDS             43748..44686
                     /locus_tag="U4A80_000375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1974046.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44714..45094
                     /gene="rplQ"
                     /locus_tag="U4A80_000376"
     CDS             44714..45094
                     /gene="rplQ"
                     /locus_tag="U4A80_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1974047.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45366..46205
                     /locus_tag="U4A80_000377"
     CDS             45366..46205
                     /locus_tag="U4A80_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1974048.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46181..47050
                     /locus_tag="U4A80_000378"
     CDS             46181..47050
                     /locus_tag="U4A80_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1974049.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47040..47837
                     /locus_tag="U4A80_000379"
     CDS             47040..47837
                     /locus_tag="U4A80_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1974050.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48040..48786
                     /gene="truA"
                     /locus_tag="U4A80_000380"
     CDS             48040..48786
                     /gene="truA"
                     /locus_tag="U4A80_000380"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1974051.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            49451..49688
                     /locus_tag="U4A80_000381"
                     /pseudo
     CDS             49451..49688
                     /locus_tag="U4A80_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(49832..50608)
                     /locus_tag="U4A80_000382"
     CDS             complement(49832..50608)
                     /locus_tag="U4A80_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1974052.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            50731..51138
                     /locus_tag="U4A80_000383"
     CDS             50731..51138
                     /locus_tag="U4A80_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEN1974053.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        16380 a         9338 c        11127 g        14638 t
ORIGIN      
        1 tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga aattaaaaat
       61 attatagaat caacactcag agaacgggtt ggtttttaat agatttaata agtactgata
      121 atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag
      181 attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat aaaactcaag
      241 aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa gaagaagcag
      301 ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa atcaattatg
      361 gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt gatgtgaagg
      421 cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc cttgggacag
      481 agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat aagcattttt
      541 caactcagct tggtggagag gaactagtgg aaatctttta accattacgc ccagcattaa
      601 taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag aaataatgtg
      661 aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg gttaaaagaa
      721 tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt atctttcatg
      781 tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt ttctttggta
      841 gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt ccaaaaacca
      901 gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac tgttttgttt
      961 ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc agcaagtaat
     1021 cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag tttgtctgcc
     1081 gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc atagacaatc
     1141 ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc aacattctct
     1201 ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg gtcaagagtc
     1261 gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag catacgataa
     1321 tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg ctctgaaatg
     1381 aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat atcctcgtct
     1441 gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc atgcagatga
     1501 gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt tacgctccta
     1561 tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca tagtgctctt
     1621 gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc aaattataaa
     1681 acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat ttcttacggg
     1741 aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta gagatcgctt
     1801 cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt cttaaattgt
     1861 cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg ccaatcagta
     1921 gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta tttgtaatga
     1981 tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta cctggtccta
     2041 agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt tctttttttt
     2101 cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa
     2161 gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat tcatctaaat
     2221 ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta acggtaagga
     2281 ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc aacattgtct
     2341 tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc taattcgaac
     2401 aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt gctgttttct
     2461 ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc gtttacaatg
     2521 caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta catgcttaaa
     2581 ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga aggagccact
     2641 aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca agggcgattc
     2701 agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga attgtgtgaa
     2761 cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt tcctgatgga
     2821 gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga agaatgtaag
     2881 aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa acgtttgttt
     2941 gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta tgccattttt
     3001 tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat ctatccagtt
     3061 gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga ttttacaaag
     3121 atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa
     3181 gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa atatgcggca
     3241 acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc gatccgtttc
     3301 ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc tcttgatgag
     3361 atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt tgaattgaaa
     3421 aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca ttttgtcaaa
     3481 gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg gacctctgtt
     3541 tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg aactgattgg
     3601 gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa aagcgattac
     3661 ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga tcagtttgaa
     3721 gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt ggaatcctta
     3781 agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat
     3841 cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt tgattttgaa
     3901 acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag tgaaaccaaa
     3961 acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga attacaaatg
     4021 cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt gaaattattg
     4081 aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga agccgacgaa
     4141 aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc atctgggaaa
     4201 gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac cactttattg
     4261 ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg ataggagaag
     4321 aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc
     4381 tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat gccagctatt
     4441 ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg ttaccaatgg
     4501 cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg tgttgtaggg
     4561 ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg
     4621 aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag tttcctgttg
     4681 ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac aaagggacta
     4741 ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg tgtgctgaaa
     4801 gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac cttccgagat
     4861 atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg cattcgacaa
     4921 atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct atttacagca
     4981 gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt ttggtttgtc
     5041 ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta tgccaatgtg
     5101 gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact gacggtgggt
     5161 ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt tccttttatc
     5221 ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc ctttattcca
     5281 gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt gaacccttat
     5341 ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc
     5401 gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc agcaccgatc
     5461 ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc ggttctaatc
     5521 gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt cttagtagag
     5581 aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt
     5641 gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga aatcaatgtg
     5701 ttggtacttt gtgctggcgc agggacaagt gggatgttag ctaatgcttt aaaaaaaggt
     5761 gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc tcattacgaa
     5821 atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta ctatgatgat
     5881 atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg agcagaatat
     5941 atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca atttcaataa
     6001 aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt
     6061 gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg aatttattcg
     6121 cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga
     6181 cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact atgttcgact
     6241 gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag ggacaaatga
     6301 tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct
     6361 ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc ctaaagcatg
     6421 gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa agaacgttct
     6481 gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca atgaactgaa
     6541 tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag aatttgatcg
     6601 attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac ctaaaacaac
     6661 tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt ggtggtttga
     6721 tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt atccgtattg
     6781 gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa
     6841 agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg atactatgcc
     6901 aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac cgacaggaca
     6961 agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata atcgcggggc
     7021 gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg caacacaagc
     7081 aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca accaagcgat
     7141 gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag caggggcgca
     7201 aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca gtccttccaa
     7261 ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg aagcgggcac
     7321 tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg catcagctta
     7381 tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg taatgtctgg
     7441 gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg aactaaatac
     7501 tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc caattacaac
     7561 aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga cgaacgttga
     7621 ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat cggaggttcc
     7681 cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa
     7741 aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc agacttcatc
     7801 tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat gaaaaaattg
     7861 caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta gaattccgtc
     7921 gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac agttcgtttt
     7981 tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata gtctcttttg
     8041 gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa gaatgttgat
     8101 ttatagggat tccccctcca tattatgttg gctctttatt cccactccac tgttgctggt
     8161 ggtttagaag tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt
     8221 acagagattt tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg
     8281 attgaagtta cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca
     8341 ccgacagagc ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca
     8401 gcagctgcga tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct
     8461 tcagtgattt cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa
     8521 acgattgaat caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat
     8581 gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg
     8641 tgagatttga tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt
     8701 ccttgtgcta ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa
     8761 acatatacga attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc
     8821 aacttgtcta agaagcgatc tcttgcattg actttgatga tgttcaagcc gaattttccg
     8881 cctagacttt ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag
     8941 atagatgtca attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca
     9001 acgccacctg aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct
     9061 acttgcatat cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag
     9121 gcaaagtggc gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg
     9181 ataccataaa aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt
     9241 ccgactgttt caaagccttc aggaacttta gttaccaagt caccatggct catccatact
     9301 gtttgttgtt ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag
     9361 gctttcccat attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc
     9421 aattgcatac catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg
     9481 atcccaaatg cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt
     9541 ggattcatcg cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact
     9601 ccaagatcac gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg
     9661 ataatctttt caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct
     9721 ttaaatttta tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat
     9781 cgaatcgatg atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg
     9841 caagatatat tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt
     9901 aaaggcttct tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa
     9961 agccgtatcc agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc
    10021 ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg
    10081 taattgaagc gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta
    10141 cttgtctgga atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg
    10201 accagatttt acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa
    10261 tcctttacgg tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc
    10321 agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag
    10381 tctggctgtt gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg
    10441 tacttctacg tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt
    10501 catataaaga taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga
    10561 aatgcggtca ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc
    10621 attacgataa aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt
    10681 catattagcc tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa
    10741 ccccaccact taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg
    10801 gagtcttatt cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa
    10861 atgagaagga tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac
    10921 gattttgaac aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct
    10981 ggtgtatttt cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat
    11041 tgggaagagt tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt
    11101 gggttagctt tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga
    11161 gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat
    11221 caatcgaata tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg
    11281 ccgatccgtg ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg
    11341 aagaagggcg gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa
    11401 aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat
    11461 atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa
    11521 ctgggcgtgc atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca
    11581 aagaactatt ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa
    11641 aataaataag atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt
    11701 tatcaaaaaa catactttcc ttttttctct attcttttct tgggggtttt ggtacactga
    11761 tagggaattt agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga
    11821 aatggcaggc gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt
    11881 tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga
    11941 ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga
    12001 tgtcactgat ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt
    12061 gggctatgtg atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt
    12121 acaagagctt tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc
    12181 tgagaaatat gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg
    12241 cggattgaat cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca
    12301 agctgtttcg ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac
    12361 tggatactat caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg
    12421 cctgcctttt aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac
    12481 tgcatcaaaa aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc
    12541 catggcgatc ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat
    12601 ctcagtcata ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac
    12661 agtgcagcag ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat
    12721 cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga
    12781 gagcgtaaaa gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac
    12841 aaaggctagt tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt
    12901 tcctaaaaat aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg
    12961 gatctgattg aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc
    13021 gtcacgaact atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca
    13081 attttttatc aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat
    13141 tctggtgatg tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca
    13201 ggtggcgttg gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg
    13261 acagtggcta aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta
    13321 gaagcaattc ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat
    13381 gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg
    13441 tatgccttgc gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt
    13501 atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac
    13561 ggtgctttca tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt
    13621 gggaaactgg ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc
    13681 gaagctatcg gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt
    13741 ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc
    13801 aaagctaaga caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa
    13861 gcgttagcaa aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa
    13921 catccagaac gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga
    13981 gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg
    14041 cgtaaaacca aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt
    14101 ggtgacactg tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc
    14161 acttctgtca ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca
    14221 acgttgattc atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag
    14281 aaaaggggaa aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac
    14341 gcatctaaag aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct
    14401 gtctgcatca atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta
    14461 gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt
    14521 gaaacaacag atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt
    14581 gctttgaaat caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca
    14641 gcaaaagatc gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa
    14701 atcatccgag cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact
    14761 ggtttctcaa caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga
    14821 ccagaaatgg gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg
    14881 atcgaagctg gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca
    14941 actggtgaag gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat
    15001 ggatcaacac agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg
    15061 taggcgcttg tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg
    15121 catcatttgg attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag
    15181 gagaaaaaac atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt
    15241 gtggtgcatg tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag
    15301 taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta
    15361 ctgaaaaaga tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat
    15421 tattttagga ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc
    15481 actagcagca gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg
    15541 tggcaaaagc tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga
    15601 cgaccgttca ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg
    15661 actttcacga ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat
    15721 cccaaatatt gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa
    15781 actaaaacca gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat
    15841 cgatgatgtc atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc
    15901 ttcttatctt gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat
    15961 cggcggtgta aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga
    16021 tgcaggtgcg aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt
    16081 ctctgctcca gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat
    16141 catcttccat gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa
    16201 cgaatcaggc gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg
    16261 cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt
    16321 tggaacagta gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga
    16381 acacactgtt tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga
    16441 cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt
    16501 tccagaaact cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga
    16561 caaggctatg gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt
    16621 ttggataagg gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag
    16681 cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg
    16741 ttcaagaaaa tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg
    16801 caaatgacaa catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa
    16861 acggtgctgg caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag
    16921 ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat
    16981 tagggattgg gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa
    17041 acatcgtgtt gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa
    17101 aagagatcca aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc
    17161 gtgatatttc agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag
    17221 cagatgtact gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat
    17281 taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata
    17341 agttagatga aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa
    17401 tcggtacagt caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa
    17461 gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta
    17521 ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag
    17581 aggttcgtgc tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt
    17641 tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg
    17701 aagacatcac taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag
    17761 aagaccgtca taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac
    17821 aaacctatca tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg
    17881 aacatgccag agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg
    17941 caaaagcttt atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag
    18001 atcctgatct gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat
    18061 ttattcataa acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt
    18121 tcgaattaga agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa
    18181 ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg
    18241 gctatacctt agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat
    18301 cgcagcgaaa aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc
    18361 ttaggcgcga tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg
    18421 ctagatgcag cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg
    18481 ttgattttta cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg
    18541 ctttctggac aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca
    18601 gatctgccaa aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg
    18661 atggcagcgg ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta
    18721 acaatcatga tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg
    18781 cctgaaagct atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg
    18841 cggttaccat ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta
    18901 gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa
    18961 atccgttctg ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt
    19021 acgatcgttt tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa
    19081 ggattaggaa cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac
    19141 ggaatggctg tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc
    19201 ttgttcagta ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt
    19261 gagatcgtca atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt
    19321 cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata
    19381 gaatcgaaac caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat
    19441 taattgcctt gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac
    19501 taggcggaac tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg
    19561 tcatgggtgc ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta
    19621 tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc
    19681 acgcagtagc aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc
    19741 tgatggcacc tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg
    19801 ataacatcac gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca
    19861 ttggaccaat tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg
    19921 tcctagcttg ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg
    19981 aaaatcctca agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg
    20041 tactgatttc tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg
    20101 gtaacttttc agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct
    20161 ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa
    20221 gcttgagtat cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc
    20281 aaattgctcc atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg
    20341 gtccaaaagc aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag
    20401 ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa
    20461 aaagaaagaa ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg
    20521 aattggagaa gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca
    20581 cattgcgaaa gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat
    20641 ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga
    20701 agaagtttct gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat
    20761 caaaactcca tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga
    20821 agaattttct ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga
    20881 cgattacggt gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc
    20941 tgtccttcag atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg
    21001 ccaattcgtt cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg
    21061 tccgtacgtg ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt
    21121 gaatccattc ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc
    21181 tgttggaaag atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa
    21241 aagcaatatg gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat
    21301 cagctttacc aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg
    21361 ctatgcgcat gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga
    21421 agaagacgac ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac
    21481 cgatcatacg cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg
    21541 ggcattgcct caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt
    21601 ttcttcgact gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg
    21661 agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat
    21721 aaaggagtaa aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca
    21781 gatgaaatcg aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg
    21841 acagtagttg ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta
    21901 gccatgcaag ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca
    21961 gtacgtgtga tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt
    22021 gtcaacgaat catttgtacc aggagatttg atgctgatta ccgatcatat caattttact
    22081 ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg
    22141 agtcaagctt atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt
    22201 ttggatctta aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg
    22261 gagatccgaa tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa
    22321 gtcatcgtag cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta
    22381 gcagcaggta tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc
    22441 aaacaatctt ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa
    22501 taaaatgagt gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc
    22561 gggagatcct ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta
    22621 caatcaggta cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt
    22681 tcaaggaaca ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc
    22741 gtatggtgta aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca
    22801 tgttcgtgat ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa
    22861 tttccaagcc tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga
    22921 gatagctaaa gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt
    22981 ctacaaagat gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga
    23041 aatggaagca gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt
    23101 gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc
    23161 tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa
    23221 tgaaaaagct gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt
    23281 tttgacttgg ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg
    23341 ttgtgaatct gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat
    23401 gtttgatgaa tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa
    23461 aaaagggact gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt
    23521 attttttagc aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa
    23581 tttcaagatc catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta
    23641 atgagttacc atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt
    23701 catcttgcca gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa
    23761 tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt
    23821 cttccattaa tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc
    23881 cgtatttttc agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt
    23941 ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt
    24001 tacatgtttt gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag
    24061 cttctttgat tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta
    24121 catcagccca accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa
    24181 aaactaattt tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc
    24241 agttctgttt tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt
    24301 ctatgagaaa acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt
    24361 ttaggtatta gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac
    24421 atgttccaac tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat
    24481 aatagagacg tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt
    24541 ttcctttttt acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact
    24601 ccatgtttgc taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga
    24661 ttccttcatc aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg
    24721 ttaattgccg cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt
    24781 gttgcatgtc agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt
    24841 catatgtctc atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg
    24901 ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt
    24961 tctagcagtg attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa
    25021 agatagtttt ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc
    25081 tttatttacg acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt
    25141 tagaaaccat gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg
    25201 tagctaagct ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac
    25261 catctgtggt taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt
    25321 cttcaaaaat atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga
    25381 acttcccaag tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt
    25441 acgtagcgac tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat
    25501 cagcactgat ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac
    25561 tctttaacgg tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc
    25621 ctgtgatacg taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg
    25681 cggttgaacc ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa
    25741 tgccagctaa ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg
    25801 ctttcctcac tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat
    25861 aaagcttttt caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct
    25921 aacaaaggtg ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca
    25981 tatttgataa ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga
    26041 tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca
    26101 attagtacgt aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa
    26161 aggatataac agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt
    26221 agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc
    26281 acgtgttcgt ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa
    26341 cttacaagaa cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt
    26401 acgttaccat atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag
    26461 ccgttctaaa tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa
    26521 cttgcttcta ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg
    26581 atgccacgta aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca
    26641 aaattagtaa ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct
    26701 aacattatct ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa
    26761 gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt
    26821 gggggttcaa actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt
    26881 cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta
    26941 gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac
    27001 actcataaaa tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc
    27061 tgtttggtcg gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata
    27121 gtaaagagag gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat
    27181 atcggtatca tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac
    27241 tatactggta aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg
    27301 atggaacaag aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg
    27361 aagggtcacc gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt
    27421 gaacgttcat tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt
    27481 gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta
    27541 tttgcaaaca aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat
    27601 gaccgtttgc aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc
    27661 acaggaatta tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca
    27721 gagatcgaag aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa
    27781 aaattggttg aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt
    27841 gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa
    27901 ttctacccag tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac
    27961 gctgttcttg attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct
    28021 aaaactgatg aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca
    28081 tttaaagtta tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt
    28141 gttttacaat caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt
    28201 cgtatcctac agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat
    28261 atcgctgctg ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag
    28321 agtctagtta ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa
    28381 cctaaatcaa aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa
    28441 gatcctactt tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg
    28501 ggtgaacttc acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct
    28561 aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa
    28621 ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt
    28681 acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt
    28741 cctcgtgaat acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc
    28801 ttagctggct atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat
    28861 gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct
    28921 aagaaagcta atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa
    28981 gattatttag gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg
    29041 gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt
    29101 ggttatgcta caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt
    29161 gaccactacg aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga
    29221 aaagctgaat aatttcttga aaccggatag tcctgcaaag agattatcca aagagactat
    29281 atttttaata tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg
    29341 tgtacatcat atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt
    29401 aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga
    29461 cacgttgacc atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa
    29521 aacggcggcc aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc
    29581 ggaatcacaa tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac
    29641 gttgactgcc caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg
    29701 gacggagcta tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac
    29761 atcctattgt ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac
    29821 atggttgatg acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca
    29881 gaatacgaat tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta
    29941 gaaggcgacg cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac
    30001 atcccaactc cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc
    30061 tcaattactg gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt
    30121 ggtgacgaag ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt
    30181 gttgaaatgt tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta
    30241 ctacgtggtg ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca
    30301 atcacacctc atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga
    30361 cgtcatactc cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta
    30421 acaggtgttg ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca
    30481 atggaagttg aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt
    30541 gaaggcggac gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa
    30601 gttttaaagt aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc
    30661 tttgttccga gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat
    30721 agaagaaaca cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat
    30781 gttttttaca agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc
    30841 gtttacttta gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga
    30901 ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct
    30961 atgtctactt ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat
    31021 ccgtttaaaa gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac
    31081 tgcaaaaaga actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct
    31141 ttacacaatc atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg
    31201 tacacacaaa cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat
    31261 gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa
    31321 taatggaggt gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat
    31381 cttcactgaa tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt
    31441 actacaagtg aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga
    31501 tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc
    31561 tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa
    31621 agaaattaca gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa
    31681 aggtaaagga ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca
    31741 cggttctcgt taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt
    31801 taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga
    31861 agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc
    31921 taaaaaatct ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag
    31981 gaggaactaa ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga
    32041 aatcacactt aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc
    32101 aatcgtaatg caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc
    32161 tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg
    32221 atctatccgc tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc
    32281 ttacagctac aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga
    32341 aaaagttgct gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac
    32401 aaaagaattc aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct
    32461 agaaaaaggt aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt
    32521 aacttctgat aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca
    32581 aactgctctt actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa
    32641 cgcccagtga tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa
    32701 gtggacactc gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt
    32761 aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat
    32821 gcaggatata caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa
    32881 atccaattat tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga
    32941 aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt
    33001 ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa
    33061 accatgctgg acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca
    33121 aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc
    33181 aaacgatcga atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg
    33241 gagtgaaagc ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag
    33301 gtccagaagc agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta
    33361 ctgttatcca caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg
    33421 gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg
    33481 gtgaagttcg tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac
    33541 aacacgaatt aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa
    33601 ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag
    33661 ctcctatcgg acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa
    33721 ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat
    33781 tactttgacc atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga
    33841 aaggaccttt tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa
    33901 agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat
    33961 ataccatcgc agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg
    34021 taggacacaa attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg
    34081 ataagaaaac gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca
    34141 tcagctaaag caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt
    34201 gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac
    34261 aaatctgctg gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac
    34321 tttgacttag atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca
    34381 atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt
    34441 catatcacgg tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca
    34501 tccaattgga atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa
    34561 agagtatgct gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact
    34621 tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc
    34681 aattcacaca gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct
    34741 aagaaaagaa ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa
    34801 aaaaccagat ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg
    34861 tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca
    34921 agggatcaaa actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg
    34981 atactcagaa ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga
    35041 agaagcagat acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat
    35101 tcttccaact aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt
    35161 gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa
    35221 gtagcattcg gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa
    35281 atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt
    35341 aaaattttcc ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt
    35401 aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc
    35461 gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca
    35521 atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga
    35581 aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt
    35641 gtttaatctt agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga
    35701 agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg
    35761 gaaggaggcc attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg
    35821 cgtggtttca gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca
    35881 ccctatctat ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa
    35941 cacagcaaaa gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa
    36001 acgtttccgt ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt
    36061 cctatataga ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag
    36121 tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg
    36181 tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc
    36241 aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa
    36301 atcaggagct cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat
    36361 tattcgtgat gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact
    36421 acgtgaaaac aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga
    36481 cacttatcaa aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt
    36541 tatcactgga aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga
    36601 taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc
    36661 tcctcaaggt ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt
    36721 tgactctact ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg
    36781 tgtttctaaa aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta
    36841 ctgaatgaac cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa
    36901 atttaactat agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg
    36961 tgttggtgat gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt
    37021 gatcactggt caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt
    37081 acgtgaagga atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt
    37141 cttagacaaa ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa
    37201 aaaagccttt gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc
    37261 agaagtagat tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac
    37321 agcaaactct gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa
    37381 ataattaaag gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc
    37441 tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt
    37501 ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat
    37561 tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga
    37621 tcaatggtca tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg
    37681 gttaaacatg agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc
    37741 ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg
    37801 atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt
    37861 atctctaaac caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac
    37921 ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt
    37981 gaaaaaaata tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag
    38041 gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac
    38101 agttactcaa gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac
    38161 tttttcagca gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa
    38221 cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt
    38281 tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc
    38341 acaattacaa ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac
    38401 accaccagct ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc
    38461 gaacaaagaa gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc
    38521 ttataaaggt aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac
    38581 tggtaaataa tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc
    38641 tgaagtttac cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac
    38701 gtatttactg cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa
    38761 aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg
    38821 gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa
    38881 ttattgatga cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt
    38941 caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag
    39001 ctgaaaaagg cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg
    39061 tgcaagcttt agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat
    39121 accattcatg gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat
    39181 taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt
    39241 tgtcggtgac aaaaacggac acgtaggatt tggtacaggt aaagctcaag aagttcctga
    39301 agcaatccgt aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg
    39361 ttcaacaatc ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc
    39421 tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc
    39481 tggggtagca gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg
    39541 cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg
    39601 caaatcagtt gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt
    39661 actttaaaac gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta
    39721 ggtcttacaa agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg
    39781 atcaatactg tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact
    39841 agaacattaa ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc
    39901 acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg
    39961 acgcggacaa aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg
    40021 gggacaaaca ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa
    40081 agactatgca gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac
    40141 tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt
    40201 agctaatggg gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc
    40261 tcaagaagca attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta
    40321 aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg
    40381 tttgtatttc gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa
    40441 aacctttctt ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa
    40501 aacttttcca ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag
    40561 cttcttcaaa tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt
    40621 cgtaaaaaac tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct
    40681 gtcggtatca ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac
    40741 gtagcaactt acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg
    40801 ctaggagaac agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca
    40861 ggtatcattt ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat
    40921 attgatcctt cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc
    40981 ttgttgatcg tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa
    41041 tatacgaaac gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca
    41101 gctggagtta ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta
    41161 caagcattga gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt
    41221 aactataata cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc
    41281 ttctacgcct ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga
    41341 agttatatac caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg
    41401 atgcgtttaa gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt
    41461 gctcaaatgg tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc
    41521 gttatcggtg tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa
    41581 tatgttggat tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac
    41641 acatttatta ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa
    41701 aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga
    41761 tatgttccgc gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat
    41821 ggataaaggt gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc
    41881 tgaaccagat acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc
    41941 aaaagctctg gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat
    42001 ccatgttggt gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg
    42061 tggagcaact taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg
    42121 cggtgggcat gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct
    42181 ggctatcaac atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct
    42241 aaacactatt gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat
    42301 tgaaaaatag cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc
    42361 aataaattat gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt
    42421 tgagaatttt gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca
    42481 agtacaagga ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg
    42541 tcgaaacttt gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg
    42601 ctactgtttc aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa
    42661 cggttgaatt gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat
    42721 tgtactccgt aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat
    42781 gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa
    42841 tccaaaacat aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca
    42901 ggagtagata ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt
    42961 ggtaacacta ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt
    43021 cgtgaattaa cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt
    43081 gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct
    43141 taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat
    43201 gcacgtactc gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt
    43261 gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag
    43321 aatatagaag caggtattgc acatatccat tctacattca ataacactat catcatgatc
    43381 actgatactc acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga
    43441 agcaaaaaat caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca
    43501 atggaacacg gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa
    43561 gcagcaattc gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact
    43621 ccagttcctc ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc
    43681 attttgagtt ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa
    43741 gataagaatg attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta
    43801 tggcaagttc atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct
    43861 acgtcgtatt ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg
    43921 tgtactgcat gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt
    43981 gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga
    44041 tattaccgga cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat
    44101 tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt
    44161 aacagtgaaa cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc
    44221 aattggcgtt cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt
    44281 tgagaacaca cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac
    44341 agatggttca attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca
    44401 tttggatatt tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa
    44461 agaagaaaca caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt
    44521 tcgctcttac aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa
    44581 atctgagcct gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa
    44641 agcaaaattg cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag
    44701 gagggaaacc aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat
    44761 gttgcgtgat ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg
    44821 tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt
    44881 gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga
    44941 aaatgaggac atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg
    45001 ttacgcagaa cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga
    45061 tggtgcacca atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata
    45121 gatgattctt ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct
    45181 gctcctcaag cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc
    45241 ttcaattacc tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa
    45301 taattgaaaa ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag
    45361 taatcatgga gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc
    45421 ttcgccccgc attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta
    45481 ttggacacaa tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc
    45541 cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga
    45601 tccgacagat ggttggaatg gtttttcaaa atccagacaa tcagtttgtt ggttcgacag
    45661 tagaagatga cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag
    45721 tgcgtgtgaa agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg
    45781 ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac
    45841 ctgatatcat tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag
    45901 tgatctctac gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc
    45961 atgatattga tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg
    46021 tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc
    46081 tagatctacc atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc
    46141 ccagtgaata tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca
    46201 agtagacttc acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa
    46261 tatgacgatc aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc
    46321 aaccttactt cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg
    46381 agaacgagat attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt
    46441 tggcatcgtc tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat
    46501 tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga
    46561 aacattagaa caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc
    46621 aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact
    46681 tgtattggat gaaccaacgg caggacttga tccccagggt agaaaagaaa tgatggagat
    46741 gttttggcgt cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga
    46801 tgatgtagcc aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag
    46861 cggggagccg caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt
    46921 acctactgcc accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt
    46981 gcctttgaca gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca
    47041 tgatgaataa attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg
    47101 atccccgagc taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata
    47161 attgggaaac gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag
    47221 tagatttgaa attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg
    47281 tagctttgca ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc
    47341 atatcactga atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca
    47401 tttttatgtc gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg
    47461 agtacttgct tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc
    47521 tgtcgattgc tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg
    47581 ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag
    47641 tcattccttt gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga
    47701 ctgctatgga agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc
    47761 attggcataa tagagacacg atggttatca ttgcttttgt attattaact gtgctactga
    47821 tctttttgag aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt
    47881 tgcaagaaag gatgcgagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg
    47941 tcacaggcct ctttctttta tctgaaaaga aagaacgaaa aaattttata aaaaaatcat
    48001 tattccagat aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc
    48061 attgcatacg acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt
    48121 caagaagaag tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt
    48181 ggttcgggaa ggacagatgc cggagtacat gcgattgggc aagtaatcca ttttgattat
    48241 ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat
    48301 attgctgtaa gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa
    48361 gaaaagacgt atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat
    48421 tatgcaagtt attttcctta ccctcttgat ttggaaaaaa ttcagcgggc gcttcctgat
    48481 ttattaggga ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa
    48541 gtacgtacga tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt
    48601 acatttaggg gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg
    48661 aaaattggca atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat
    48721 cgtaatgcag caggacctac agcacatcct gaaggacttt atttgtatga agtgaaatat
    48781 gaataagagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat ttacgaaaat
    48841 tacttctcaa aattttgcaa aatttcggct tattttcgaa ggagctactt ctggaacacc
    48901 gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaaacaaaa
    48961 atagctcgtc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    49021 ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    49081 aaaaatagtt cctcatattt tgccgtttgt tgagctaata ccgaaggtca gctgattgaa
    49141 caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa gtaacaattt
    49201 caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc gttgataagt
    49261 gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac gttgttttga
    49321 ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt tatatttaaa tggcacaaat
    49381 ttttctaatt atttgtcatt tagcgctgaa aatttttgct atttatctaa aaaagtgata
    49441 actacttaag atgaagcaga atatttacgc catacggcat acaaccgcga aaaatataaa
    49501 aaaaagaaaa cgattgaacg cgtttttgca gtcgcaaagg aaaagcatgg aatgcgctac
    49561 atcaagtatc gagacttgga aaacaatcag gtgtatacgc tgattgtttt tgctgcaatg
    49621 aatctgaaaa agttgtccac ttggcagttg gaacatatgt cttcatatct tttattatac
    49681 aaaaatagca aaagcaccag tctacactct tagtaaagag gaaactgatc cttttgtcta
    49741 cattcttaaa gtacttaaac taagtacttt attttactct ttaggattga tacagtagca
    49801 tctatccctt tattaataat cttttatttt tttaaataaa gatgttgtct ccatcttcag
    49861 gcactaaaac tttttcggaa attccttttt ctttattgaa agactttaat tcttctcttg
    49921 acagtcccca atggtttaca gcttccatat gaacggcaat aattgtggaa tctggtattg
    49981 ctttatgcaa ttcatataaa tcgtctttcc ccataataag agattctcca actaaaaaat
    50041 gattatctcc agcatttgct actatgattt ctggattata ctgatcaata gtttgtttaa
    50101 cagcttcata ccaaactgta tctcctgtaa agtatagtgt cttctcagtc gaatgtttga
    50161 aaacaatacc gcaaacatta cctacataat tcaagatttc tcctctgcca tgtttgcctt
    50221 ttgttttaac caattgtata gattcaaaaa ctgtgtcttc tcgtagtacc tccacatttt
    50281 taaaaccata tagtcgaatt tcattcgcat cattttcatc ttgcacaaat aattttattt
    50341 ctttaggtag gacctctttc gctatatcgt cccaatgatc tagatgtaaa tgtgttacaa
    50401 taacagcatc aataccgttg ataatatctt cgactggcat tggcaaatca actagaggat
    50461 tcatttgatc ttttcttggt gatggaaaag gacctttcga aataaatggt ggaagtgttc
    50521 ctttttccgc taacattgga tcaattaaaa attttttacc tccatagtct aagatagctg
    50581 ttgcattacg aatgtgatgt atattcataa acgttgtctc ctttttgttg atatgaaata
    50641 tttttagtat aattcaattc catacaataa ataaaccgaa caaaaaaagt cttttactat
    50701 ttttacttaa tttatgaaag gttgtttacc atgttagacc aaacagatat aaatatcatt
    50761 gaggaactat caaaaaatag tcgaatcaca atgaaagaac ttggagaaaa agtacatttg
    50821 actggacccg ctgtctcggc gagagtgaca aaactagagg aaagtggagt tattgaaaat
    50881 tatacagtaa aattaaatat ggaaaagcta gggttttcga ttcacgcttt tttaattata
    50941 attacgcaaa atttaaatca taaaccctat ttatcattta ttaaaaatca tgaaaaattc
    51001 ttgattagaa attataagat tagtggaggg ggctgttatc ttttggaatg tagatttact
    51061 tcaaaaaaat caatgaacag ctttttggaa cagcttaatg aatatgcgaa ttataaatta
    51121 tctattgtta tagattaata ataaaactag cagatggtaa aaatactaat atgcgagaca
    51181 cacattcaac cataactata taacttatca atttaaaact aaactgatgg aaacaagtaa
    51241 ggcaaaaaca tttatatttt gccctactta tttttttaat taaattcatt gatgattcat
    51301 atttagaaaa ttctatgaag gccaaatgag tatcctgtca cttttttaga taaatagcaa
    51361 aaattttcag cgttaaatga caaataatta gaaatatttg tgccatttaa atataaaaat
    51421 gacaccaagt atatcatgaa cgcacaaaaa gaccaacagg gtataccctg ttggtctaaa
    51481 taa
//
LOCUS       DASJRQ010000007        50004 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJRQ010000007 DASJRQ010000000
VERSION     DASJRQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.007"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(222..707)
                     /locus_tag="U4A80_000384"
     CDS             complement(222..707)
                     /locus_tag="U4A80_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1974054.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="U4A80_000385"
     CDS             1187..1828
                     /locus_tag="U4A80_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1974055.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="U4A80_000386"
     CDS             1847..2482
                     /locus_tag="U4A80_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1974056.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="U4A80_000387"
     CDS             2500..3315
                     /locus_tag="U4A80_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974057.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="U4A80_000388"
     CDS             3634..5070
                     /locus_tag="U4A80_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1974058.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="U4A80_000389"
     CDS             5259..7706
                     /locus_tag="U4A80_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1974059.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="U4A80_000390"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="U4A80_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1974060.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9196..10299)
                     /locus_tag="U4A80_000391"
     CDS             complement(9196..10299)
                     /locus_tag="U4A80_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1974061.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="U4A80_000392"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="U4A80_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1974062.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11946..13607
                     /locus_tag="U4A80_000393"
     CDS             11946..13607
                     /locus_tag="U4A80_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974063.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13677..14024)
                     /locus_tag="U4A80_000394"
     CDS             complement(13677..14024)
                     /locus_tag="U4A80_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1974064.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14108..15049
                     /locus_tag="U4A80_000395"
     CDS             14108..15049
                     /locus_tag="U4A80_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974065.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15050..16093
                     /locus_tag="U4A80_000396"
     CDS             15050..16093
                     /locus_tag="U4A80_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974066.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16099..17169
                     /locus_tag="U4A80_000397"
     CDS             16099..17169
                     /locus_tag="U4A80_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974067.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17159..18112
                     /locus_tag="U4A80_000398"
     CDS             17159..18112
                     /locus_tag="U4A80_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974068.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18124..19134
                     /locus_tag="U4A80_000399"
     CDS             18124..19134
                     /locus_tag="U4A80_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1974069.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19419..20240)
                     /locus_tag="U4A80_000400"
     CDS             complement(19419..20240)
                     /locus_tag="U4A80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1974070.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20337..20615)
                     /locus_tag="U4A80_000401"
     CDS             complement(20337..20615)
                     /locus_tag="U4A80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974071.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20883..22892
                     /gene="metG"
                     /locus_tag="U4A80_000402"
     CDS             20883..22892
                     /gene="metG"
                     /locus_tag="U4A80_000402"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1974072.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23371..23958)
                     /locus_tag="U4A80_000403"
     CDS             complement(23371..23958)
                     /locus_tag="U4A80_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974073.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24133..25455
                     /locus_tag="U4A80_000404"
     CDS             24133..25455
                     /locus_tag="U4A80_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1974074.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25610..26386
                     /locus_tag="U4A80_000405"
     CDS             25610..26386
                     /locus_tag="U4A80_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1974075.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26379..26948
                     /gene="rnmV"
                     /locus_tag="U4A80_000406"
     CDS             26379..26948
                     /gene="rnmV"
                     /locus_tag="U4A80_000406"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1974076.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26941..27825
                     /gene="rsmA"
                     /locus_tag="U4A80_000407"
     CDS             26941..27825
                     /gene="rsmA"
                     /locus_tag="U4A80_000407"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1974077.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28276..28905
                     /locus_tag="U4A80_000408"
     CDS             28276..28905
                     /locus_tag="U4A80_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974078.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29029..30138)
                     /gene="ugpC"
                     /locus_tag="U4A80_000409"
     CDS             complement(29029..30138)
                     /gene="ugpC"
                     /locus_tag="U4A80_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1974079.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30347..30769
                     /gene="mgsA"
                     /locus_tag="U4A80_000410"
     CDS             30347..30769
                     /gene="mgsA"
                     /locus_tag="U4A80_000410"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1974080.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31092..31628
                     /locus_tag="U4A80_000411"
     CDS             31092..31628
                     /locus_tag="U4A80_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1974081.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31668..33431)
                     /locus_tag="U4A80_000412"
     CDS             complement(31668..33431)
                     /locus_tag="U4A80_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974082.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33434..35149)
                     /locus_tag="U4A80_000413"
     CDS             complement(33434..35149)
                     /locus_tag="U4A80_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974083.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35928..36152)
                     /locus_tag="U4A80_000414"
     CDS             complement(35928..36152)
                     /locus_tag="U4A80_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974084.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36343..39120
                     /locus_tag="U4A80_000415"
     CDS             36343..39120
                     /locus_tag="U4A80_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1974085.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            39201..39620
                     /locus_tag="U4A80_000416"
     CDS             39201..39620
                     /locus_tag="U4A80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1974086.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            39632..40102
                     /locus_tag="U4A80_000417"
     CDS             39632..40102
                     /locus_tag="U4A80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1974087.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            40119..40898
                     /locus_tag="U4A80_000418"
     CDS             40119..40898
                     /locus_tag="U4A80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1974088.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            40885..41706
                     /locus_tag="U4A80_000419"
     CDS             40885..41706
                     /locus_tag="U4A80_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1974089.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            41723..42772
                     /locus_tag="U4A80_000420"
     CDS             41723..42772
                     /locus_tag="U4A80_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1974090.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            42785..43789
                     /locus_tag="U4A80_000421"
     CDS             42785..43789
                     /locus_tag="U4A80_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1974091.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(44378..45550)
                     /locus_tag="U4A80_000422"
     CDS             complement(44378..45550)
                     /locus_tag="U4A80_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN1974092.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            45754..46773
                     /locus_tag="U4A80_000423"
     CDS             45754..46773
                     /locus_tag="U4A80_000423"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1974093.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            46816..47697
                     /gene="galU"
                     /locus_tag="U4A80_000424"
     CDS             46816..47697
                     /gene="galU"
                     /locus_tag="U4A80_000424"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1974094.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            47997..48374
                     /locus_tag="U4A80_000425"
     CDS             47997..48374
                     /locus_tag="U4A80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974095.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            48980..49594
                     /locus_tag="U4A80_000426"
     CDS             48980..49594
                     /locus_tag="U4A80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1974096.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        16346 a         8727 c        10332 g        14599 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagctat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    11581 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    11641 cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct
    11701 tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga
    11761 agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga
    11821 aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat
    11881 taaactctta aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac
    11941 gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg
    12001 gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg
    12061 gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg
    12121 caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg
    12181 atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg
    12241 gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta
    12301 cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat
    12361 atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata
    12421 aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac
    12481 aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg
    12541 tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac
    12601 cattcacatt agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa
    12661 atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa
    12721 aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt
    12781 ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc
    12841 ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc
    12901 taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg
    12961 acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga
    13021 acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag
    13081 aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg
    13141 cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa
    13201 acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct
    13261 caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa
    13321 gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg
    13381 cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc
    13441 gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg
    13501 tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg
    13561 cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt
    13621 gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat
    13681 cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc
    13741 agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc
    13801 tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa
    13861 gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca
    13921 ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga
    13981 atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat
    14041 ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga
    14101 aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac
    14161 gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata
    14221 tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt
    14281 ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt
    14341 tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg
    14401 accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt
    14461 aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc
    14521 gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc
    14581 aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc
    14641 aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat
    14701 ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg
    14761 ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg
    14821 accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc
    14881 tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc
    14941 tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta
    15001 cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat
    15061 accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa
    15121 aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc
    15181 atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat
    15241 cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt
    15301 ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa
    15361 agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt
    15421 aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg
    15481 aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt
    15541 agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt
    15601 tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt
    15661 tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc
    15721 acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt
    15781 gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag
    15841 tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt
    15901 aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga
    15961 agggtacaag acattcctgt atctgcctta tctattatgg attccggcag taactttatc
    16021 ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa
    16081 aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt
    16141 gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga
    16201 gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc
    16261 atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac
    16321 gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg
    16381 attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct
    16441 gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa
    16501 ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag
    16561 ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa
    16621 atactgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg
    16681 gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac
    16741 cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt
    16801 gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac ttggggactg
    16861 cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct
    16921 ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg
    16981 atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca
    17041 gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt
    17101 cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat
    17161 gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa
    17221 tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt
    17281 agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga
    17341 acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa
    17401 aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt
    17461 gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc
    17521 taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa
    17581 tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg
    17641 tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc
    17701 actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca
    17761 tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg
    17821 aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa
    17881 tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac cagatccaga
    17941 ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg
    18001 tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata
    18061 ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca
    18121 tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg
    18181 attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa
    18241 agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa
    18301 tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat
    18361 cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa
    18421 agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc
    18481 tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa
    18541 ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta
    18601 ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat
    18661 catccgaaga cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac
    18721 cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct
    18781 caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta
    18841 atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg
    18901 aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat
    18961 gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa
    19021 tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta
    19081 agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg
    19141 aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg
    19201 tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat
    19261 agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta
    19321 cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt
    19381 ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg
    19441 agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa
    19501 tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag
    19561 aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga
    19621 acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga
    19681 ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag
    19741 atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag
    19801 cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag
    19861 ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc
    19921 tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt
    19981 tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa
    20041 ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac
    20101 atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga
    20161 taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct
    20221 ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta
    20281 ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa
    20341 atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat
    20401 actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg
    20461 ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa
    20521 taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca
    20581 atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat
    20641 agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata
    20701 tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag gatagattga
    20761 acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct
    20821 ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc
    20881 ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac
    20941 ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt
    21001 tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa
    21061 caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg
    21121 tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg
    21181 attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt
    21241 acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac
    21301 aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg
    21361 gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag
    21421 accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa
    21481 acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa
    21541 cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg
    21601 atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg
    21661 aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga
    21721 tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc
    21781 atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc
    21841 ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg
    21901 ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg
    21961 atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt
    22021 gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta
    22081 cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata
    22141 cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag
    22201 aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc
    22261 atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc
    22321 cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac
    22381 actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac
    22441 gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa
    22501 caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa
    22561 aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact
    22621 gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc
    22681 aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg
    22741 ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc
    22801 aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa
    22861 aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg
    22921 tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact
    22981 tgaagccaat catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa
    23041 gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag
    23101 aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt
    23161 ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct atatttatat
    23221 ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt
    23281 gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat
    23341 gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt
    23401 tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata
    23461 ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa
    23521 caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac
    23581 ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat
    23641 ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat
    23701 accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac
    23761 aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga
    23821 taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg
    23881 ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata
    23941 agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga
    24001 aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa
    24061 agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga
    24121 aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct
    24181 accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct
    24241 agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc
    24301 tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta
    24361 aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc
    24421 ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg
    24481 aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct
    24541 gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg
    24601 attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa
    24661 ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg
    24721 agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg
    24781 gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt
    24841 tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat
    24901 catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc
    24961 ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat
    25021 acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt
    25081 aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag
    25141 ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg
    25201 atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa
    25261 aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt
    25321 gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca
    25381 ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa
    25441 cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag
    25501 atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc
    25561 tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga
    25621 ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg
    25681 agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga
    25741 aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc
    25801 gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt
    25861 accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa
    25921 agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc
    25981 aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga
    26041 tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg
    26101 atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt
    26161 agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc
    26221 cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta
    26281 tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac
    26341 atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag
    26401 agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag
    26461 atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg
    26521 cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa
    26581 gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag
    26641 ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag
    26701 aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta
    26761 gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa
    26821 aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga
    26881 agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac
    26941 atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat
    27001 ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg
    27061 aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga
    27121 atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc
    27181 gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt
    27241 gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag
    27301 gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg
    27361 cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta
    27421 gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt
    27481 caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag
    27541 ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg
    27601 acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg
    27661 ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca
    27721 aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa
    27781 gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga
    27841 cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    27901 cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt
    27961 aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac
    28021 agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat
    28081 tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat
    28141 caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt
    28201 gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat
    28261 taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg
    28321 attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc
    28381 tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca
    28441 gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca
    28501 ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat
    28561 ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc
    28621 aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac
    28681 tttattggta atatgcctga aacatattat tctcaaggat atttttatac agtattcatc
    28741 ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata
    28801 gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat
    28861 cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa
    28921 aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta
    28981 aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt
    29041 gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc
    29101 gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga
    29161 accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga
    29221 tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg
    29281 acgaataccg aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt
    29341 tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt
    29401 cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg
    29461 tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac
    29521 gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt
    29581 ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc
    29641 taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc
    29701 caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc
    29761 tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata
    29821 tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc
    29881 gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt
    29941 tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt
    30001 agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga
    30061 tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat atacgttttt
    30121 cagagccatt tctaccataa tacgttccac actttctttt ttagttttat taactattta
    30181 aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt
    30241 ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac
    30301 actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt
    30361 gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat
    30421 attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac
    30481 cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc
    30541 catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca
    30601 gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt
    30661 agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga
    30721 ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt
    30781 atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata
    30841 atatgcgata taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt
    30901 tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag
    30961 agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg
    31021 taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg
    31081 agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat
    31141 tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa
    31201 tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag
    31261 ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct
    31321 ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc
    31381 gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca
    31441 acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg
    31501 tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca
    31561 tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg
    31621 ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg
    31681 aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg
    31741 cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg
    31801 gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt
    31861 tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata
    31921 gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc
    31981 tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca
    32041 tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca
    32101 aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca
    32161 aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt
    32221 gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc
    32281 cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg
    32341 atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg
    32401 atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg
    32461 atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca
    32521 gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca
    32581 ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat
    32641 aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt
    32701 gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct
    32761 tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc
    32821 gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg
    32881 atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga
    32941 cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga
    33001 ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg
    33061 atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat
    33121 gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg
    33181 agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac
    33241 agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt
    33301 aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg
    33361 tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt
    33421 tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt
    33481 gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga
    33541 tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa
    33601 gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag
    33661 agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct
    33721 gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg
    33781 gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt
    33841 ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag
    33901 aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat
    33961 ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg
    34021 tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca
    34081 gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat
    34141 ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa
    34201 gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg
    34261 tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat
    34321 tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa
    34381 ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg
    34441 cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact
    34501 tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct
    34561 gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg
    34621 tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca
    34681 gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg
    34741 ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg
    34801 tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat
    34861 tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag
    34921 catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata
    34981 agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag
    35041 ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca
    35101 aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc
    35161 tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt
    35221 tagaatgaaa agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag
    35281 aaattgaata gactattttt tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca
    35341 ttcataggaa cgtctatttt acttaatttt tctataaatg aattctgcat aaaaaatata
    35401 tcaagtaaat gtttatagat gaaactctat ttacttcttt tttaatttgt tcttgcaatc
    35461 ttttttgcta cgtaattttt gtaaaaaagt gggttgagag aaatctaatt gattttcaaa
    35521 gaaggagtat gaaaatgaga aaaatattta ttcgtttatt gtgggtacta tcttagagga
    35581 ataaataata gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga
    35641 agacaacctc ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa
    35701 gaaaacaagg taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact
    35761 aagagtggtg tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga
    35821 aagcatcata actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag
    35881 gatttgtaat agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg
    35941 aaggatttat agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata
    36001 tattgcgtat ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc
    36061 aaaaatacaa ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta
    36121 tgtttcctgt ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc
    36181 atatgttgaa attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt
    36241 ataagggata agtaggctgc tgtctataat aagatatatt tttcaattct ttattaaaga
    36301 ttttattgac aaaatgaatc cgttttctat gatggaggag gggtgaataa gttgttgaag
    36361 aaggaaattt tatgttactt agagaaccag acagcctttt tcgatttaga acatatgaac
    36421 gaggtcttta cagcacaaag gttagctgat cattttgggg taaaaagaaa cacagtcagt
    36481 cattatttga accagttaaa tgatgagcaa ctactagtca aaatcaattc tcgaccagtg
    36541 atttatttcc acaaagaagc ttttgaaaaa caatttttta agttacgcac gaatttttat
    36601 ttttctatcg atgatctcaa agaggaacag ccttttttcg cgcaaccaaa agacttattc
    36661 tcttttatga tcggatacga cgccagtttg aaagaaagta tcgaacaaat caaaacagct
    36721 ctttactatc cagatggcgg attaccactt ttgattacag gcgaaagtgg cacaggtaaa
    36781 agttatttag ttaatttggt ctatcagtat tgtctgcttc atgatctgct agaagattct
    36841 gcaccattca tcacttttaa ttgtgcacaa tatgcagata atcctgaact tttgactagc
    36901 aatttgtttg gacatgttaa gggagcgtac actggtgcag aagaaaataa aaaaggggct
    36961 tttgaagcag cagatggtgg gatcttgttt ttagacgagg tacatcgctt aacaccagaa
    37021 ggtcaggaaa aactgttcac ctatcttgat caaggtgtga tttatcgtat gggagaacct
    37081 aatttgtcac gccggatcaa aacacgtctc ttttttgcta cgactgaaga gattactagt
    37141 catttcttaa caactttcat tcgaagaata cctatccgaa tcgaactacc tgcacttaat
    37201 cagcgtagtc gtaatgaacg gctagaactg atttactctt tttttctcga agaacaaaga
    37261 aaaatgggga gccctttgtc agtcagtgga ccagctttgt cacttttaac aagtcagaaa
    37321 ttatccggaa acataggaga gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat
    37381 gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc atcatttgca aaaagaacta
    37441 cttgcgcagc ctattactaa aaatacagca cagcaaggaa tctatattac gcaagaccag
    37501 accttagagc aattaatgga gaagcaacag cccgaacagc agcgaatcgt taaaagtttt
    37561 gagcaaatcc taattgattt caagaaaaat aactgtctgc tatattcttg cgaaggaaaa
    37621 ttaaagcaag aagtgattca attatttgat tttttgctgt ttgagaccag tagacaagaa
    37681 aaacacgagc tgctggttta ttggacacaa tctatccgag agacctttcg acaaatggaa
    37741 actgcctatc aaatcaaatt cgatggaaac agtgtctatg ccttgagtta ctatctctat
    37801 cagcgaagca ctatcaaatg gttaccggaa gaacatgata ttatccaact aatcaaagaa
    37861 ttagaacaac aagtttctca gtcttatcca tctagttatc actatgtcca acgaattctg
    37921 gaattaagta agatcaaatt agatattgaa gacacaagta tggatcggat tttactgacg
    37981 atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct
    38041 gctcacggtt atgcaactgc tagcagtatg gcaaatgtag taaatcgttt attacaagag
    38101 gatctatttg aatcatttga tatgccacta gatgtcacac cacagcagat tgcttcagaa
    38161 atccttgatt attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt agtagacatg
    38221 ggatctctga aagaaatcca tcagtttttt aaaaaacagt tatccgtacc gcttttgatt
    38281 ttgaataatg tgacgacacc tttagcaatc acagtagggg aatgcctgca aaaaaatgta
    38341 tcattagaag agatagcaga agagactgtt cgacagatcc agccagaatg gcgactgctc
    38401 tatccagagg aaaacaaacc caaagcactc attactactt gcttcactgg aatcggtacg
    38461 gcggtccact taagtgaact gctagaaaaa agcttaccta cggcttgtca gttgaaaatc
    38521 atcccatatg aatatcagca attaaaagat aagaaaaaca gtgacccatt attttccata
    38581 tacgaagtcg taggaatgat tggcaccaca gatcctgata tcaccaatat cgactatctt
    38641 tcattagaag acctcatctc gggggaaaag atgtctgtac tagctgaatg gttggacact
    38701 actatgaatg cttcagagaa ggaaatattc aatcagcgca tcattcgtaa tttttcattg
    38761 gaaaaagtac tggattccgt gactattcta gatacagaga aggtgatggg agaaatcgat
    38821 cattttatga gagaattaga ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta
    38881 gcgttgtatg tccatgtgag ctgtctcatt gaacgattga tccgacaaat gccaatcgaa
    38941 acctatcaag gattagagaa atggaagcag tgtcaaaaag aagggttatc tgctattaaa
    39001 tctgcgttta gtgtcattga agagcattat agtgtcatga tccccaattc tgagattgcc
    39061 tatatctatg atattttgag tgaagataca gagtttctct cgatagaaga agaattttga
    39121 acataagcaa gttgttgcca ctaattttgg catgcaactt gcttttttag tatgtgtaag
    39181 tgaaaagagg agatgaaaaa gtggatagag aaattatctt agcttcccat ggaaaatttg
    39241 cttcagggat attagattca ttagagctga tctatgggaa aaatcattct attactgtat
    39301 tcgattgcta tactgatgag aattttgatt tagcggaaac agtaaggcag ctttttatga
    39361 ggtacaaaga taaagaaatc attgttctga cggatctttt tggcggaagt gtcaacaacg
    39421 aattcttaca atatatcaat cgggatcatg tctatctcgt tgccggattg aatttgccac
    39481 tattgattga gctagtatct cggttggacg ctgaagtaga gacaactgtt ttgatccagc
    39541 aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa
    39601 tagaagaaga cgaattttag gaggaagaaa aatgattgta ttgacaagag tcgaccatcg
    39661 gttattacac ggacaagtag ctttttcatg gacacaaaca ataggcgcag actgtatttt
    39721 gattgctaat gatgatgtac ctacgaatga gatacgaaag acaacgatca aattagccaa
    39781 gccacaagga gtcaaactag tgatcaaatc aattgatgac tcgattgccg cgttgaaaag
    39841 cggggtgaca gataaatata agttattcat cgtagtggaa tcgatcgagg atgcatataa
    39901 gctggcagaa gcgtatccag aaatcaaaac catcaactta ggagggatca aggcaaaacc
    39961 tggtactcgc agcatcggaa aagcggtaaa tattttacca gaagaggagg aacgcttgaa
    40021 acgattaatt gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt
    40081 ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat gattgtacaa gcagtcttat
    40141 taggaattgt tgcatttgtc gcacaatcag aatatgcact agggacatcg ttgctttctc
    40201 gtccgattgt gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa actgggatca
    40261 ttatgggcgc aactttagag ttagcattta tcggatcgtt ctcagtaggt gcttcgattc
    40321 cacctgatgt tgtgaccggt gggattttag ggacggcttt tgctattaca gctggagccg
    40381 gtacagaaac cgcgctttta ctaggtttgc cgattgctac gttgacattg atattgaaaa
    40441 atatttactt agggcttttg attccaatta tgaaccataa agcagacaat tatgcagaag
    40501 aaggaaatta taaaggagtc gagcatatgc acttgcttgc cggtatcggt ctttccttga
    40561 tgcttggtgt tgtggtaatg gtttcttata tggtgggaag caatacgatc ggaaatctat
    40621 taaatatgat tcctgacttc gttcaaaagg ggttgtcagt agctacaggg ttgattccag
    40681 ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca ccatattttt
    40741 tcttaggttt tgctatagct tcttacttag agattcctgt gaccggagta gctatttttg
    40801 gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa
    40861 caactgcagg ggaggtagaa gaagatgaag aattctgaga tcaaaaacag caaaataacg
    40921 aaaaaagagc taaatcacgt attttggcgt tcttttcaaa tggagttttc ttggaattat
    40981 gaacgtcaga tgaatatggc ttatgtttat gcaatgattc cgattttgaa aaagttatat
    41041 cagtcgaaag aagaaatggc agcagctttg aaacggcatc tagagttttt taacacgacc
    41101 ccacatatcg ttacactgat tttaggaatc acggctgcaa tggaggaaga aaataaggat
    41161 gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa catctttgat gggacctctt
    41221 gcagggttag gagattcttt cttttgggga actttgcgat tgattgccac aggtgtgggt
    41281 acctctttag ccatgcaagg aaatatttta ggccctatct tgttcatttt gattttcaat
    41341 attccgcaca ttctgtttcg ctatctagca acaggttggg gatatcgctt aggaacgggt
    41401 tttttgaaaa agatccaggc aaacggtatg atggaaagtt tgacattagg cgcgtctatt
    41461 attggactga tggtcgttgg agcaatgact gccacgatga tcaccatcaa tattccttta
    41521 aaaatcggtt caggagaaaa tgctgtgact gttcaaagta tttttgatga tatcgttcca
    41581 aatatcttat gtcttggagc atttggcata gtgttttatc tattgaagaa agaagtgaaa
    41641 ccattaacga tcttgatcgg actagctttc tttggaattt ttggttcgtg gatagggatt
    41701 ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat tatatctatg aagaaaagga
    41761 agtactaagt acgatcttga aaaagaatga tttttcgaca agagaacata tgaaaaaaac
    41821 agtgaattta ctgatcttag caacgggttc atcttataac gcttgtttag cagcgaagcc
    41881 agcattggaa tcttatggtg atcttactgt cgatattcaa gaaccttttt attttttcaa
    41941 ttatggaaaa ctttctcctt caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag
    42001 tgcttctact gttgaagcag taaaaatgat acaaaaacaa gggttacctg ttgttgctat
    42061 cacaaatgac gtgcaaagtc cacttgcttt agaagctgca caaattattg atttaggtgc
    42121 cggggtagaa tcagtaggct tcgttacaaa aggctattct gcgactgttt tacagttact
    42181 actcttaggg ataggaattg gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga
    42241 ctacatgcaa cagttgcgca aaataatcaa ccatttacca gcgattattc aaaaaacgga
    42301 agaatttttt gatggatatc aaagtttatt tcgtttagca cagcgtttcg tcaccattgg
    42361 atatggtcca aactggggaa cagctaaaga agcagagacg aaattgactg aaacaatccg
    42421 cgttccgtca caaggatttg agttagaagc ttatatgcac ggcccatacc tggaggcaga
    42481 tgcttcgcat ttgctattct ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa
    42541 gttgcaaaga tatatgagtc ggtacgttgg agagactctc accattacca caaaaaaagc
    42601 aagaaacgag aaaacacttg gattagcaat agaatgtgat gaatatctgt ctgtattagc
    42661 gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca gtagctaaag gtattgactt
    42721 gaacaaaaag atttttgaag actttgatac agtcttgaaa agtaaattat aagaggtgga
    42781 aatcatgctg aaattcaatg aagaagaaca aatcaaagca ataaaaggcg cactagcttt
    42841 acgtccgcaa gtagaagaaa tcatcgataa actttatgcg gaaaaatttg acgctgtcta
    42901 ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg
    42961 gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa tattacacta ctggaaacaa
    43021 aagattaaca aaagattcta tcgttatttt gtcctctgtt accggtacaa ctcaagaagt
    43081 agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca ctgattggat ttatcgataa
    43141 agcgaacagt aagttgtctc agctatgcga ttttgtggtc acttatccag ctcctggaac
    43201 agaacaaatc aaatttttta tggcagcaga tcgattgatg tatttgaatg gagaattcga
    43261 agcttattca gaatactatg aacagctcga acgttattta ccaacagggt tagtggaagc
    43321 agagaaaaaa gcagatgcat ttggcttatc atttgcagaa aaacatcgac gtgatagtat
    43381 gcattatttt attggagcag ggaatcaatg gggagctgtt tattcatatg ccatgtgtta
    43441 ctgggaagaa caaagctggc ttccatcgaa atctatccat gctgccgagt ttcttcacgg
    43501 gacgttagag atcgtagaag aaacaacccc agtcacatta tttttaggcg aagatgaaca
    43561 gagagtgtta tctgaacgtg tggctaaact tttgccaaaa atttgcagca attatacatt
    43621 gatagacaca aaagactatc cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt
    43681 gtctttctta ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg agcagttgaa
    43741 ctgccatcca ttagagatac gccgctacta tcgtcagttt gattattagg atagactgga
    43801 taaaaagaat ataaaaaata aagagagcag cgataactgc tccctttatt tttttaaaat
    43861 attggcatca attacgttat aaaacacatt ttttgcatta tctacagttc aacttataat
    43921 ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta tgcaatagta ctatttagtt
    43981 gttgcatgga tccgtagtaa aagtccaagt acaacgagtt gcgccagttt acatagcgtt
    44041 ttgtatccag aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc
    44101 tgtccgttgc aggacctgac agatttagaa atgttcaatc tgtctcttat gtattttaaa
    44161 tcaatactca ctaggagaag tttcctaacc tcctaatagt aatgctggta gtaacattgt
    44221 caaaactgct tcctcttagg aacaagcaaa gcataacgag tgtaaaaata aaatgtagac
    44281 tattgtaaca tatcattgat tatttcaagt aaaaacgata aaagaaagtc aagtccataa
    44341 tcctgtgtaa aatactatac aatgttttac cggtctctca ttgatcaaac ttaatatatt
    44401 ttcttgtaga actgagccat tcgtcatgaa ggtccataag gacagctcca attaatcgat
    44461 tggcagacgt tcggttggga aaaatccgaa taatcttttc tcttctgcgg acttcctggt
    44521 tcagtcgttc aagaaggttg gtgcttttta gccgattatg actatttccg ataaccgtgt
    44581 attgaaaggc atcttcgaag ccattatcca atatttcgca ggcttttgta tatttaggct
    44641 ggtcgatata ttcaccgact aaggcattct tagctgttcg agcgagttca atatccgtaa
    44701 acttaaagat cgcttttact gcttctctaa acggttttga attctttttt ggaatggaac
    44761 tgaagatgtt tcttaaaaaa tggacctggc atctctgcca acttgcgttg gtaaatgact
    44821 tacgaatcgc agacactagg cctttatggg catcagaaat gatgagttct gtccccttta
    44881 ggccgcgttc ttttaagtat tcaaagaaga tggaccatgt gtcatcactt tcttcatttt
    44941 gaatcatgaa gccaatgatt tcacggtcgc caccttctgt tatcccgatc gcaatatggc
    45001 agcttttaga aagcactcga tggtcctcac ggacttttat gtagagaaca tcagtcatca
    45061 gataaggata attcgtacct gagagtgaac ggttctgcca ttcgttgacc atcgggtcta
    45121 actgctcagt caggctagaa acaaaagatt tcgatacaga ttttccacat agctcttcaa
    45181 caatctttga aactttacgt gtcgaaacgc ccgaaacata catctcaagc attgaagcga
    45241 gcagtgcctt ttcatttcgc tgataacgct caaacaccgt cggtgaaaat tcaccatcac
    45301 gtgttctagg cacttttaat tcgagtgtac ccacacgagt cgtaaagtct cgctcataat
    45361 agccatttct ttgactctga cgactttctg aacgttcata gtcatcggct tgaatatatt
    45421 ctgttcgttg attttccatc aattggttga aaacagtggt taaaatattt ttagaaacat
    45481 catcttttac cgaatgttca ataatacttt gaacctcttc gttgttcagt gtaaaatgta
    45541 cttgggtcat gtaacgtcct cctgggtatg tttttgtggt taaaaacatt gtaccgtaaa
    45601 agggctgtta catggccttt tatcttttac acaattatat ggactttatc taaaagaagt
    45661 aaacaaacac ttaaaaacaa tggaaattag tcccaacttt aagaaatgta tactataatg
    45721 gaaattatca attatttatt tggaggaata tccatgaaac aaaaaatcgc agttcttggt
    45781 cctggatctt ggggcactgc tttagcacaa acattggcag aaaacgggca tgatgtccgt
    45841 atttggggaa atgtacctga acagattgat gaaatcaata agtatcatac aaatcagcat
    45901 tttttacctg atttgacgat cccagaatcg attatcggat ataaagaatt ggcagaagca
    45961 gtggataacg cagatgctat tttatttgtt gttccaacaa aagcgattcg ttcagtcgca
    46021 caggaactta ttggtaaaat gaatactaag ccaatcatta tacatgcaag taaaggacta
    46081 gaacaagata cgcataaacg aatctcagaa gttttggctg aagaaattcc agaagaaaaa
    46141 agacaggcaa ttgttgtatt atctggtcct agccatgctg aggaagtggc agtacatgat
    46201 atcacaacaa ttacagctgc aagtattgat caaaaagcag cagcctatgt acaaaaactg
    46261 ttcatgaatg attatttcag gatttataca aataatgatg tgattggcgt agaaactggt
    46321 gcagcactga aaaatatcat tgcaattggt gccggcgcta tccatggatt aggattcgga
    46381 gatgatgcaa aagcagcaat catgactcga ggattggcag aaatcagccg tcttggtgtc
    46441 gcgatgggtg ccaatcctct gacatttatc ggtttgagtg gtgtggggga cttgatcgtt
    46501 acttgtacaa gtgttcattc tcgcaattgg cgtgccggta acttgttagg acagggacat
    46561 aagctggaag aagttctaga aaacatggga atggtcgttg agggtgtggc aacgacaaaa
    46621 gcagcagtag agctagctca acagctaggt gttgagatgc caattactca aacaatctat
    46681 aatgttttgt ataatggaga agatatcaaa aaagcagcaa aagaaatcat gttacgtgac
    46741 ggaaaaatgg agaatgaatt ttctcttcgt taaatagatg atcaatagtt aatcgtcgtt
    46801 taggaggaac agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca
    46861 cgttttttgc cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca
    46921 acgatccagt ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta
    46981 acgggaaaag aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac
    47041 aatttgcggg aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc
    47101 aatcttcact tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca
    47161 aaagcattta taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat
    47221 gatatcccat tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt
    47281 gccgtcatgc gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca
    47341 gatagcgaaa agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa
    47401 gcaccaagta atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata
    47461 ttagaaactc aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact
    47521 cttaaccaga cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc
    47581 gataagttag gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg
    47641 acttcattaa gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg
    47701 gaaataagtg gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgcgt
    47761 catttgcgca actgtcgttt tagttttttg actctagatc agttgagtcg tggaaaacga
    47821 aagatagaaa aagactttcg tgaatagatt cagaaaatat tatgctaatg gtttaatttt
    47881 tttttaagtt gtataaaaga aaatgattac ataagagaaa tgaaagccaa gccagaagat
    47941 tcatagtaga ataaaaaggc gaatttttta attataaaaa aaggtgtgtg ttcactatgg
    48001 gaaaattagt cgctgttgca cagtcgctgc ttgatatact caagtggctg atggaaggaa
    48061 gcaattggat atttgtgttg ccactattgc ttattagtct atttatcatg tttcgcatgg
    48121 tcagaaaaaa agaaacgttt ctgattttta tctggtatgc agcatccgtt ggggttgcta
    48181 ccttgatggt tgaattggcg ttaggtagtt gggaagcttg cgtatttgct ttattttttg
    48241 ctgtactgta tctaatcatt tatatcccat atatgaaaag aaagtctcgg ataggcagaa
    48301 aaggtagaca tacaactact ttttctagta cagattctct cgaacaaagt agaagaacga
    48361 gaagaagacg gtaatattta gtaggtatac gcaaaaaaag ataaaataat ggataaacta
    48421 ttgagatgag agatttattt aattggtgga aaaaagcttt tttcacttat ataaaagaat
    48481 cttttttaaa ggaatgcttg ttgtatcggg ataagccatt ccttttttta tgttatttat
    48541 atatatataa atcataccaa tgtttttttg tattttcttg cttgcacttg gataagagat
    48601 tacttacaat aaattcagat agaaacagct gaataaaaaa aggctccctg acccttagtt
    48661 ggcgctaaga atcagggaat gaccagtcac acacatacac cctgaccgat cattttgcca
    48721 tagtcaatgc agtgcatcga cctaatttat tgtacctaat ctagggtgaa aattctaccc
    48781 ctatgtagat attttccatg gtctgcacgt tgttttattt gctatgtcaa ggtattgggc
    48841 gtattcgttc aatgcctttt ttgtgtttga aacagcaaag accacatgtg ttactgtcta
    48901 actactgttt ttttatgtac ttccttagat taggcagctg tttctattca tgtcaagtat
    48961 agctaggcgg gagagataaa tgagtttatg ttattttgac tattatcacc aattggaaga
    49021 gaaattaagg atagaagagc cgctgatgct tcaagctggc acttggatcg cgaaacaaat
    49081 taatcaaggt ggtagattgc agatatatgc tagccgcaca ctaagtggtg tagcatttga
    49141 attttgggat caaaccccgg atcttgtacc aagtatattg attgaaaatc cagccggagg
    49201 tgtctatgaa acgctagaag gtgcaggaca ggcaattatt gatcagcttt ctgtcaaatc
    49261 agaagacatt tttcttcttc tttcgaatga aggaagaaac ccggcaatca tcgaactagc
    49321 tcagtggatc aaagaaaatg gtcatccatt aatcattgtt actggatttg atttgtcacg
    49381 aagtatcaaa tcgcaacata gcagtggttt gcgattattt gaatttgctg atttggtttt
    49441 ggacaaccac gccaatttac atgatgcagt cttgactttg ccaaatctag atccagcgat
    49501 ctgcggaaca gcctctcttg caacgatctt tttgctgcag caagttttgt attttacagc
    49561 aaaacagcta gtccaggaca caagtggaaa atagttatcc acatgttgac aaagttcctt
    49621 aaaaaaataa tgttccacat aaaaatggag cgtttgaaaa aaagaaaacg attattaatg
    49681 aggattaaca acgactactt tttttgtttt agttatttta agatggatca tctttgattt
    49741 taagcttgtt ttgtttaaag tttgttattt ttataagttg tttcacagtt atgagaaatg
    49801 agttataaga atatgtagtg tcagaaaaat aaaaagtagt ggaaaaagag acaagaaaga
    49861 atccatatgg attctttctt gtctcttaat acacgttgat gaaagagtct tccccaaaac
    49921 tctttacaaa gtaattatag tacatttgtt atcattattg caatgaagtt tacctgatat
    49981 aacaaaattg tccaacagga tacc
//
LOCUS       DASJRQ010000008        49829 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJRQ010000008 DASJRQ010000000
VERSION     DASJRQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.008"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..222)
                     /locus_tag="U4A80_000427"
     CDS             complement(25..222)
                     /locus_tag="U4A80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1974097.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(616..2343)
                     /locus_tag="U4A80_000428"
     CDS             complement(616..2343)
                     /locus_tag="U4A80_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1974098.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2336..3529)
                     /locus_tag="U4A80_000429"
     CDS             complement(2336..3529)
                     /locus_tag="U4A80_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1974099.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3716..4360)
                     /locus_tag="U4A80_000430"
     CDS             complement(3716..4360)
                     /locus_tag="U4A80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1974100.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4454..4588)
                     /locus_tag="U4A80_000431"
     CDS             complement(4454..4588)
                     /locus_tag="U4A80_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1974101.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4590..6722)
                     /gene="feoB"
                     /locus_tag="U4A80_000432"
     CDS             complement(4590..6722)
                     /gene="feoB"
                     /locus_tag="U4A80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1974102.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6719..7192)
                     /locus_tag="U4A80_000433"
     CDS             complement(6719..7192)
                     /locus_tag="U4A80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1974103.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7593..7817
                     /gene="nrdH"
                     /locus_tag="U4A80_000434"
     CDS             7593..7817
                     /gene="nrdH"
                     /locus_tag="U4A80_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1974104.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7976..10135
                     /gene="nrdE"
                     /locus_tag="U4A80_000435"
     CDS             7976..10135
                     /gene="nrdE"
                     /locus_tag="U4A80_000435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1974105.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10185..11150
                     /gene="nrdF"
                     /locus_tag="U4A80_000436"
     CDS             10185..11150
                     /gene="nrdF"
                     /locus_tag="U4A80_000436"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1974106.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11239..12378)
                     /locus_tag="U4A80_000437"
     CDS             complement(11239..12378)
                     /locus_tag="U4A80_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1974107.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12687..13400)
                     /locus_tag="U4A80_000438"
     CDS             complement(12687..13400)
                     /locus_tag="U4A80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1974108.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13654..14355)
                     /gene="nagB"
                     /locus_tag="U4A80_000439"
     CDS             complement(13654..14355)
                     /gene="nagB"
                     /locus_tag="U4A80_000439"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1974109.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14589..16121
                     /locus_tag="U4A80_000440"
     CDS             14589..16121
                     /locus_tag="U4A80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1974110.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16312..17514)
                     /locus_tag="U4A80_000441"
     CDS             complement(16312..17514)
                     /locus_tag="U4A80_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1974111.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17596..18285)
                     /locus_tag="U4A80_000442"
     CDS             complement(17596..18285)
                     /locus_tag="U4A80_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1974112.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18391..18759)
                     /gene="rplL"
                     /locus_tag="U4A80_000443"
     CDS             complement(18391..18759)
                     /gene="rplL"
                     /locus_tag="U4A80_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1974113.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18812..19315)
                     /locus_tag="U4A80_000444"
     CDS             complement(18812..19315)
                     /locus_tag="U4A80_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1974114.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19519..19646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19725..20414)
                     /gene="rplA"
                     /locus_tag="U4A80_000445"
     CDS             complement(19725..20414)
                     /gene="rplA"
                     /locus_tag="U4A80_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1974115.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20518..20940)
                     /gene="rplK"
                     /locus_tag="U4A80_000446"
     CDS             complement(20518..20940)
                     /gene="rplK"
                     /locus_tag="U4A80_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1974116.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21283..21951
                     /gene="sdaAB"
                     /locus_tag="U4A80_000447"
     CDS             21283..21951
                     /gene="sdaAB"
                     /locus_tag="U4A80_000447"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1974117.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21964..22836
                     /gene="sdaAA"
                     /locus_tag="U4A80_000448"
     CDS             21964..22836
                     /gene="sdaAA"
                     /locus_tag="U4A80_000448"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1974118.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22927..23946)
                     /locus_tag="U4A80_000449"
     CDS             complement(22927..23946)
                     /locus_tag="U4A80_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1974119.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(24119..25024)
                     /locus_tag="U4A80_000450"
     CDS             complement(24119..25024)
                     /locus_tag="U4A80_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1974120.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25083..25628)
                     /gene="nusG"
                     /locus_tag="U4A80_000451"
     CDS             complement(25083..25628)
                     /gene="nusG"
                     /locus_tag="U4A80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1974121.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25729..25899)
                     /gene="secE"
                     /locus_tag="U4A80_000452"
     CDS             complement(25729..25899)
                     /gene="secE"
                     /locus_tag="U4A80_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1974122.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25923..26075)
                     /gene="rpmG"
                     /locus_tag="U4A80_000453"
     CDS             complement(25923..26075)
                     /gene="rpmG"
                     /locus_tag="U4A80_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1974123.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26237..26881
                     /locus_tag="U4A80_000454"
     CDS             26237..26881
                     /locus_tag="U4A80_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1974124.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26937..27440)
                     /locus_tag="U4A80_000455"
     CDS             complement(26937..27440)
                     /locus_tag="U4A80_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1974125.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27453..27499)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28037..29002
                     /locus_tag="U4A80_000456"
     CDS             28037..29002
                     /locus_tag="U4A80_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1974126.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29101..30273)
                     /locus_tag="U4A80_000457"
     CDS             complement(29101..30273)
                     /locus_tag="U4A80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974127.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30260..31405)
                     /locus_tag="U4A80_000458"
     CDS             complement(30260..31405)
                     /locus_tag="U4A80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974128.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31419..32343)
                     /locus_tag="U4A80_000459"
                     /pseudo
     CDS             complement(31419..32343)
                     /locus_tag="U4A80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32460..33539
                     /locus_tag="U4A80_000460"
     CDS             32460..33539
                     /locus_tag="U4A80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1974129.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33571..34164
                     /locus_tag="U4A80_000461"
     CDS             33571..34164
                     /locus_tag="U4A80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974130.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34274..34501)
                     /locus_tag="U4A80_000462"
     CDS             complement(34274..34501)
                     /locus_tag="U4A80_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1974131.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34604..37192)
                     /locus_tag="U4A80_000463"
     CDS             complement(34604..37192)
                     /locus_tag="U4A80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1974132.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37212..37871)
                     /locus_tag="U4A80_000464"
     CDS             complement(37212..37871)
                     /locus_tag="U4A80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1974133.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37920..38423)
                     /gene="trmL"
                     /locus_tag="U4A80_000465"
     CDS             complement(37920..38423)
                     /gene="trmL"
                     /locus_tag="U4A80_000465"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1974134.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38644..39510)
                     /locus_tag="U4A80_000466"
     CDS             complement(38644..39510)
                     /locus_tag="U4A80_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1974135.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39619..40260
                     /locus_tag="U4A80_000467"
     CDS             39619..40260
                     /locus_tag="U4A80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1974136.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40322..41683)
                     /gene="mgtE"
                     /locus_tag="U4A80_000468"
     CDS             complement(40322..41683)
                     /gene="mgtE"
                     /locus_tag="U4A80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1974137.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41701..42600)
                     /locus_tag="U4A80_000469"
     CDS             complement(41701..42600)
                     /locus_tag="U4A80_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1974138.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42602..43399)
                     /locus_tag="U4A80_000470"
     CDS             complement(42602..43399)
                     /locus_tag="U4A80_000470"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1974139.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43377..44081)
                     /locus_tag="U4A80_000471"
     CDS             complement(43377..44081)
                     /locus_tag="U4A80_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1974140.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44212..44788
                     /locus_tag="U4A80_000472"
                     /pseudo
     CDS             44212..44788
                     /locus_tag="U4A80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44906..45583
                     /locus_tag="U4A80_000473"
     CDS             44906..45583
                     /locus_tag="U4A80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1974141.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45662..47470)
                     /gene="pepF"
                     /locus_tag="U4A80_000474"
     CDS             complement(45662..47470)
                     /gene="pepF"
                     /locus_tag="U4A80_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1974142.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47646..48644)
                     /locus_tag="U4A80_000475"
     CDS             complement(47646..48644)
                     /locus_tag="U4A80_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1974143.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48870..49523)
                     /locus_tag="U4A80_000476"
     CDS             complement(48870..49523)
                     /locus_tag="U4A80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1974144.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14909 a        10467 c         8757 g        15696 t
ORIGIN      
        1 atttgcttag catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc
       61 acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt
      121 tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac ccccatagat
      181 aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa
      241 ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg
      301 aaacgtagca aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt
      361 atattcttta atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt
      421 ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg
      481 cgtctttgga cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata
      541 gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat
      601 ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca
      661 gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat
      721 atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt
      781 caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc
      841 ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac
      901 aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga
      961 tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt
     1021 aactacacca gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc
     1081 atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa
     1141 attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt
     1201 aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat
     1261 tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac
     1321 tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt
     1381 ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc
     1441 accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt
     1501 attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa
     1561 tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc
     1621 gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat
     1681 tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa
     1741 tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac
     1801 ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc
     1861 aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa
     1921 tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct
     1981 ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag
     2041 aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag
     2101 accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac
     2161 cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga
     2221 catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa
     2281 ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca atagattatt
     2341 catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga
     2401 caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat
     2461 tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt
     2521 cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt
     2581 tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg
     2641 tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt
     2701 tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat
     2761 ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca
     2821 tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa
     2881 tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca
     2941 gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag
     3001 ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt
     3061 taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt
     3121 taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa
     3181 acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt
     3241 ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa
     3301 tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca
     3361 cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg
     3421 ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg
     3481 tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc
     3541 ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg
     3601 tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa
     3661 atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt
     3721 ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc
     3781 caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca
     3841 gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact
     3901 cgctaattga acggtccaga tgcattgctt tttctgataa agataattca aacggaagta
     3961 aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa
     4021 aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt
     4081 ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa
     4141 caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag
     4201 ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga
     4261 tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct
     4321 gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt
     4381 ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc
     4441 ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt
     4501 catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga
     4561 ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta
     4621 taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt
     4681 aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga
     4741 taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc
     4801 catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa
     4861 agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact
     4921 tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg
     4981 ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt
     5041 ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt
     5101 tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata
     5161 ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt
     5221 tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga
     5281 gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca
     5341 gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct
     5401 gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc
     5461 gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca
     5521 catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa
     5581 agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag
     5641 cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg
     5701 atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg
     5761 ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt
     5821 gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc
     5881 aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa
     5941 gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg
     6001 accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg
     6061 atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca
     6121 aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt
     6181 tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc
     6241 ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg
     6301 ctgattggta aaactggtac accaagacca taggaaagct tttctagatt gatttttttt
     6361 ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc
     6421 aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg
     6481 atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa
     6541 ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac
     6601 gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca
     6661 gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc
     6721 attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt
     6781 cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta
     6841 tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt
     6901 ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt
     6961 cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca
     7021 ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt
     7081 tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga
     7141 ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc
     7201 ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg
     7261 tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc
     7321 aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa
     7381 atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga
     7441 tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac
     7501 acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag
     7561 ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata
     7621 attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag
     7681 aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa
     7741 gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc
     7801 gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc
     7861 ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa
     7921 tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag
     7981 tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt
     8041 caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa
     8101 tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga
     8161 tcaagaatat atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct
     8221 atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt
     8281 ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga
     8341 tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct
     8401 tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg
     8461 acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa
     8521 ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg
     8581 gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc
     8641 aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt
     8701 aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc
     8761 tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct
     8821 tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt
     8881 atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac
     8941 agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg
     9001 tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa
     9061 tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg
     9121 ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt
     9181 attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag
     9241 ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc
     9301 atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg
     9361 attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga
     9421 ggattcatta gactttacaa atatttactt catgctgttg aattattgga cacttgttga
     9481 aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta
     9541 tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa
     9601 agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa
     9661 acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg
     9721 ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga
     9781 agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga
     9841 gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta
     9901 tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga
     9961 aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa
    10021 tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt
    10081 tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca
    10141 tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt
    10201 atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc
    10261 tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc
    10321 gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc
    10381 tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc
    10441 atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt
    10501 cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa
    10561 atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta
    10621 caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt
    10681 tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca
    10741 aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag
    10801 gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc
    10861 tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct
    10921 ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag
    10981 ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga
    11041 tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag
    11101 gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt
    11161 taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca
    11221 ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt
    11281 ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta atattatcgt
    11341 cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc
    11401 tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa
    11461 caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata
    11521 caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa
    11581 agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata
    11641 aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa
    11701 gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa
    11761 ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc
    11821 cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat
    11881 aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt
    11941 actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc
    12001 ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg
    12061 ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac
    12121 taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt
    12181 ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg
    12241 taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat
    12301 aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg
    12361 tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt
    12421 aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata
    12481 ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat
    12541 actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc
    12601 agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg
    12661 acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg
    12721 atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga
    12781 tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc
    12841 acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct
    12901 tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc
    12961 actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc
    13021 atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc
    13081 gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc
    13141 acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct
    13201 aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct
    13261 cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca
    13321 aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt
    13381 gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag
    13441 cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt
    13501 catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa
    13561 acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct
    13621 gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc
    13681 gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac
    13741 aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac
    13801 gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc
    13861 ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac
    13921 tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc
    13981 gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca
    14041 tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt
    14101 atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc
    14161 aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat
    14221 ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt
    14281 gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac
    14341 acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc
    14401 tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca
    14461 ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat
    14521 gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag
    14581 gagttattat gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc
    14641 tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa
    14701 acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg
    14761 gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg
    14821 atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg
    14881 aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg
    14941 aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct
    15001 tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga
    15061 aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc
    15121 cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc
    15181 tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat
    15241 ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata
    15301 ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc
    15361 aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag
    15421 ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc
    15481 tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca
    15541 cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac
    15601 ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg
    15661 gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc
    15721 acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat
    15781 tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac
    15841 gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat
    15901 taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt
    15961 tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag
    16021 acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc
    16081 aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg
    16141 aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc
    16201 aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt
    16261 tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt
    16321 attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg
    16381 aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc
    16441 tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg
    16501 gttgcttgct gcttcaccta gcccctgtac tcgtgtttga ttggcgactt gaccaaattg
    16561 attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac
    16621 caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc
    16681 agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa
    16741 aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa
    16801 aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc
    16861 ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta
    16921 tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt
    16981 aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc
    17041 aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt
    17101 acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat
    17161 ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga
    17221 aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa
    17281 ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc
    17341 taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc
    17401 tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat
    17461 tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt
    17521 cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac
    17581 tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata
    17641 aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt
    17701 gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac
    17761 tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa
    17821 attgaccata gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg
    17881 atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa
    17941 ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt
    18001 gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt
    18061 aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa
    18121 agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa
    18181 taattggatg tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat
    18241 gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa
    18301 aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca
    18361 taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc
    18421 ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc
    18481 aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac
    18541 tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt
    18601 ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga
    18661 tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc
    18721 ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt
    18781 gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg
    18841 ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca
    18901 tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag
    18961 agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt
    19021 cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc
    19081 cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag
    19141 aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta
    19201 cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt
    19261 tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg
    19321 agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt
    19381 atagttactt tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct
    19441 actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct
    19501 cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg
    19561 actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact
    19621 gtcttcggta caagttaatt attaacctca actagcttag catgctaaac tagttgagtc
    19681 aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg
    19741 tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct
    19801 ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact
    19861 aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc
    19921 acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta
    19981 actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga
    20041 cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt
    20101 tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct
    20161 tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt
    20221 aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat
    20281 gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct
    20341 tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg catttttttg
    20401 ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt
    20461 gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta
    20521 ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac
    20581 gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga
    20641 aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt
    20701 tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta
    20761 tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc
    20821 ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc
    20881 tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac
    20941 gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac
    21001 gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca
    21061 gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga
    21121 ataaacgcta aatttattct aaaaaaacaa caatttttcg ttctcatttc ttcttttatg
    21181 ttacacttgc gctagcatgg ttcaacagat gaaagcaaac aataacacgg ttttttagac
    21241 ccttgtacaa agattcctac ataacaaaat gaggtggaga aaatgaaaga tttaaggtat
    21301 agaagtgtct ttgatattat tggacctgtg atgattggtc caagtagctc tcacactgcc
    21361 ggagctgcta gaatcgggaa aatcatacgt agtatatttg gcgaacagcc tgattctgta
    21421 gatatttatc tatatgaatc atttgcaaaa acctatcggg ggcacggaac agatattgca
    21481 ttagttggcg gcctgttagg gatggaaccc gatgatgaac gtttagctga ttctttgaaa
    21541 ttggcatatg aagccggtat ggaagtcttg tttgttccta agagtgaaaa agccgaacat
    21601 ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa agctttcagt cactggcatt
    21661 tcgatcggcg gtggcaatat ccagatttct gaattgaatg gattcaaaat ttctttaagc
    21721 atgggtactc ctaccttgat cgttgtccac caagatgttc caggtatgat tgctaaagtg
    21781 actagtatac tttctgagac caatacgaat atcggcacta tgacagtgac acgggaatca
    21841 aaaggtgaaa atgccattat gataattgaa gtagatgatc ctcaagtaga agaaacggta
    21901 aaaagactca aacagctgcc aaatatcgac agcgtaaatt atttcgatta aggagctaaa
    21961 aaaatgtttt tatcgattga agaattagta gatcaagcaa aagcttatcc aagtgtagca
    22021 gaattgatga ttgcagttga aatggaaatg agtggccgtt cccgtagtca catcatcgag
    22081 atcatggaaa aaaacctctc agtaatggaa caatcaatca acgagggaaa agaaggtgtt
    22141 acctccgtca caggaattac cggtggtgat gcaaaaaaat tagatgatta tttaaatgag
    22201 gcagactttt taagtggaga aacaatctta aaagctgtac gcaatgctgt agctgttaat
    22261 gaagtcaatg caaaaatggg gttgatttgt gctacaccga ctgccggaag cgcgggtgtt
    22321 gttcctggtg tgctgatggc tgtccgagat cggcttgatt tgacacatga acagcaattg
    22381 gaattcttat ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc ttctatcagt
    22441 ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc agcagcagct
    22501 ttggtttgtg caaaaaatgg gactcctcat caagcagcac aagcagtagc cattacattg
    22561 aaaaatatga tgggactgat ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc
    22621 aaacgtaatg ctttaggctc ttctcaagct tttatctctg ctgatatggc attagcgggt
    22681 atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa
    22741 atgccgtcga ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc aactgctaaa
    22801 cgacttgcgg aagagatttt aaataagagg gcgtgataaa gctccttaat ttcgagtatt
    22861 aagagattag ttatcttcat aacaaaaaac gaagagagga ctaattgccc ttctcttcgt
    22921 tttttattaa tccatcaatt tcgtatattt tacttcatcg atcgtttttg ttcctgcaag
    22981 ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa acagatgtta ctccttctgc
    23041 cccacctaga ttcaacccat aaatcactgg tcggccaatc gcaactaagt cagcaccact
    23101 tgccagagct ttgaagacat gctctcctcg acggacacca gagtcgaaaa tcactggtac
    23161 acgtttgttg actacttcgg caatttttgg caatacttca aaggacgcag gacctccatc
    23221 tagttgacgt ccaccatgat tggataccca gataccatct gcccctgcag agatagcgat
    23281 tactgcatct tctggcgatt gtattccttt tacgataact ggaagatggg tgatttcttt
    23341 gattgttttg atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat
    23401 tccttttcct tcgccattac cacttgcact ttgttcgcta tacgcagcta agttaggcat
    23461 tggtaatgga aattggaatt gattgatgac gtcttcttca cggtaaccac cgagcgttga
    23521 atctgccgtt aaaatgatag ctttagcccc tgcttctact gctttgttca aaatgaactc
    23581 attgaatctg tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc
    23641 atcggctgcg tctttgattg tcgtatttgc ataagtgctg atggaaaaga ttgatccagc
    23701 agcagctact cctttagctg tatcagcttc ccctttttca tgtgccaatc cttgggccgc
    23761 tgatggcgct tggataatcg gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc
    23821 ggctgagtcg attccgcgta atatccgtgg catgattttc tttgtattaa aagaagtcgt
    23881 attttctttc atcgtccact catcttcaga accaccgcga atatagccaa aagctcctgc
    23941 ttccatgcgc tcttttacac gaccttctag ttaggcaatg tttacaatat cgattggatg
    24001 ttcttcatta cttgtatgat aagtcatagt agatccttct ttcatcttct tgattacata
    24061 taaatcatac taaacttaca aaaagtaagc aaacatttac tctcagatac ttttatcgct
    24121 attctttcca aataaatcga cttacaagct gatctacttc ttggttttca tgaagcatac
    24181 tatgagaggc attttcacct gtcactactt tttcctctat ttcatttccg cgttgcttca
    24241 acaacgcata gaccgctaat gcactgttta acggtactgt tccatcactc aagtctgttt
    24301 catctagttg accagcaatc aagaaaaatc gagtggttat aggaatatta ccgatcattt
    24361 gctggtaatc ttgatagcgg ttagaaacga cagcaggccc ctttgctaac tcatctgtca
    24421 gagactgcgc actatcatcg acaaaatcat taaaaggcgc gccaattgct gttaatgtct
    24481 gaacttgtgg caatgacgta tcctgtccat acgttcctaa atagcgtagt gcactaactc
    24541 ctcccatgga atgccccact atgttgactt tatctatgcc gtagttttct ttcagataca
    24601 aaagacaagt tttgatccat tctgcttgat tccactcgtt atttttatta tcttcaaaca
    24661 atacttggac catcggattg tctgcttttc cagacaaatc tcctgttgct tgtatttgtc
    24721 catctgttcc tactgttaaa accaattctt ttactgcttg ctgatttttt tccaaacgcc
    24781 gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa
    24841 tagtggtttt ttctatcact ttactggatt ctgccgtttt ttctttatca gaaacttcag
    24901 tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata aggactaaag
    24961 ctgctgcagt tgcacgtttg cataaatttt tgattggttc tgtcatacac ctcttccttt
    25021 ccatatagaa aaaagaaaga cttgaaaggc caatcatatg atcgacattt caagtctctt
    25081 tattaatcga ttttatctac ttgatcgaaa tcaagttctg tactggtttc acgtccaaac
    25141 atatcgatgt tgactttcaa tttttctttt tctccgtcaa cttctgttac ttgtccttct
    25201 aatccagcaa atgcgccttc gatgattttc acgacttcgc caacttctac ttctagatca
    25261 ttttgtcgca ggctcatacc gatagaacgc aagatatgat tgacttcttc tggaagtaat
    25321 ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg
    25381 cggacaacat accatgaagc gtctgtcatg atcatttcaa caagtacgta tcctgggaat
    25441 gttttatgaa cgatttcttt ttctttccca tttttgactt cagtttcttt ttcttcagga
    25501 acaacgacac ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga ttcgatattt
    25561 gcttttactt tgttttcata tcctgaatat gtatgcaaga cataccagtt tttttcaaaa
    25621 gattccattg aaggctcctc atttcattgt aaaataaaaa aacctcagta cacccgaagt
    25681 ttttctttcg cttcattata gcactaaaag tacgttgtgg ctagcgagtt attttaaaat
    25741 ccagccaaat aagctttgga tggctgtatc cattacgtaa aacatcaatc cgaaaataat
    25801 agaagtttcg atgactacta acgtatcttt acggagttgt tttctgcttg gccaagttac
    25861 ttgtttgatt tcatctttta cgctttttag aaatttcatg agacaacctc ccttgctact
    25921 gtttatttcg tttctttatg taatgtatat tgattacaat atttacaaaa cttattgatt
    25981 tccaaacgtt cgccgctttt tccttcactg actgattttg tgtagttgcg agacccacaa
    26041 acggaacaag cgagtgctgc ttttttcttt gccatttact tctgccccct tgctgagaat
    26101 cagacttata cttcataaca gtatcacgta actttttact tgtcaatttg tgaaataaac
    26161 tatctattgt tttggataaa attatgatat gattaatttg aaattaagct aaaatgagac
    26221 aattggaggt atagcgatgc tattaaaaac acttgtatat aagaaaaaag atctgacaac
    26281 cgttacagag actagtacat tagaagaagc attaaaaatt ttagaaactt ccggataccg
    26341 ttgtgtgccg atcttagatt cttcaggtaa tattttccgt ggaaacattt acaaaatgca
    26401 tatctatcgt cataaagcaa atggcggtga tatgacgctt cctgtcactc atcttttgaa
    26461 aaacgctaca aagttcattt acctggatac ttctttcttc aaagtattct ttacgatcaa
    26521 agagcttcct tatattgctg tccttgacag tgaaaatcat ttttatggta ttttgacaca
    26581 tagtacgttg cttaatatgc tctctcaatc ttggagcgta gaacaaggaa gctatgtact
    26641 gacaattgct tccacaggaa agcaaggaga tttggctgct attgcaaaaa tcatttcaaa
    26701 acacagcagc attgccagct gtattactct tgatatcgaa aaagacgaat acattcgacg
    26761 tacattgatc acgctaccgg ctaacactga agcgaaagta tgtgaaacga tcgtcaacca
    26821 cttagagaaa aagaattata aagtggtaga aattgaaaat ttgcaaaacc aagaagcata
    26881 atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt ttataattag
    26941 acacgctttt caaaatcaag cgcgcgatct aaataataca tcaatggtgc tgaaacagcc
    27001 atgacgataa actcacttaa tgcaagcgtg ccataggttg tccaaaatgc ttggccacct
    27061 gttggaacta acatataagc aatcagtccc atgctgatgg taaagaagat cgtgttcaat
    27121 actaaacgca tctttacatt tttcactttg ttttgcaata aagctgtcag gcctaaagaa
    27181 agcaatgttt ggccgccacc aaaaaccaca tccatgatac caaaaccaaa gaaggcatta
    27241 tagacgatga ccccgcctaa aactccccaa agcaatttac ggttaaagac aactaaatga
    27301 ttcaaacttt ctgaaatacg aaactggatc ggccctgagg atacaggcgc aactaaaata
    27361 gttaacgcta gataaagagc catgatgatg ccatttacaa cgattacgga taaccgttgt
    27421 ctgcttttcg ttttttgcat tttctctcct cttttctcct agttttttta acgcgggatg
    27481 gttgatgaac cgcggttatg tgaaacaaac aataattata acagactttt catttatttc
    27541 aatccgtctt atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa
    27601 atcgtgggat atggtataaa gtaagtgtaa ggggtcataa gaattcccag taaagcagac
    27661 gaagtaggat ctgcaagaac agaattgttg tgaaacttaa ataaataaaa aaggatgtgg
    27721 gaatttgagc aacctcttac tagagaacgg tctagcaagc agctgaagat ctaagtggac
    27781 atgccgaacc ttggaacttc tccttacgta agtaatggat catgcgcttg ctaggctttt
    27841 ttgtgttttt cggtcttcgg acctttttca ttttcctaga cttctgctat aataaccaag
    27901 aattaatttt gtaacagatt tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa
    27961 aagcttgcta attattggtt aaccagcaaa caggagtatt aaaatatctt gttttattga
    28021 atagcaggag gaaatcatga aattccatta tcatatattg atcaatccat cggcaggcag
    28081 cggaaatggc tataaagtag cagaaagaat tttaccggta ttaaaaaata agcatattga
    28141 ctatacactt cattatagtg aatacaaagg acacgatgct gagattgctg aaactttggc
    28201 aaaagaaaca ttgattcctt ggatagaaga agaacagaaa acaatcgata ctttccccct
    28261 tttgatcatc gtaggaggtg acggcacctt acatcaggta cttgatactt tctatcagat
    28321 ggaagtagaa ttcccagttg cttatattcc agcaggttct ggagatgatt ttgctcgagg
    28381 ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg attttagcag ctcaatcacc
    28441 agaaaaagtc cacataatgg cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt
    28501 taataatttc ggtataggat tagatgcagc tatcgtccat gcgacaaatc attcttccac
    28561 aaaaaagcga ttgaacaaat acaacttagg atcattttcg tatttattct ctattctccg
    28621 tgctttattc actcaaaaag gatttccaat tctggtagat gcaggaggaa aacaattaag
    28681 tttttcaaac gcatttttat gtacagcaac gaagcatcct ttttttggcg gaggaatcgc
    28741 gattgttcca actgctgatg cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat
    28801 caatttcttt aaaatcttgt ggctcattct tttacttata cagaaaaaac aattgaaatc
    28861 gaaatatttc catcactaca caacaaacag attacgtatc gtttcaacaa caccgcaaca
    28921 tggtcaagaa gatggagaag aaatggaaaa acgacctttt gatatcacaa taaccacaac
    28981 taaacagttg ttttggtgtt aataaaagag gttttgtcag aagctacatc tggaacaccg
    29041 tttattcgga aaaaaggact gtgacacgac ttttgtcaca gtcctttttt tactatgcag
    29101 ttaattcttt gcccaattgg gttgccatcg atagtttctt tctttaccca aaataaattg
    29161 gatcattgaa aacaccgcag caaaagcaac taagacacaa ccattgaacc aaaaagattc
    29221 tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag ttattatttt gaacaaacca
    29281 atatgttggt gtgaatgccg ctattttgct aaccatttct ggcaagtatt ctgctggtac
    29341 aaacacccca ccgataaagc aactgcctgt gataaaaata tctttgatcc caccgctggt
    29401 ttctgagttt tttaccaatg aagtgataca agccgaaaag ctaatgatcg gcaataaaaa
    29461 caagaaagta tttaaaatat gataccatac cgttatatcc cacgaggtgt ggatcactaa
    29521 cagaaccatt agaagtaaga agccactgaa caacagcagc gagtaactaa tcaaactaaa
    29581 ggttactttg cgattcaagt ttcgtttggt aatgggcgca ctctggtttc gtttactgat
    29641 ctctttgcga ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa
    29701 cccataagac aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc gctgactata
    29761 tcccgcactt agctcaactt ctccagtaac cgagagtgcc atttgtgtct gttccatagc
    29821 tgcatcgatt gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac
    29881 ttgctgtgta acaactgttt tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg
    29941 catctttcct acttcgactt gttcggtaaa gtctgcaggc agatgtaaga taatgtttac
    30001 acgattaaaa taaagagcat catcgatttc tttttgtgag gttccatcca atgtaacgac
    30061 tgtttccttg tcattcaaat atttacgaag gccatcgact acaggcgact cttcttcact
    30121 aatcaaggcg attttcgcat cttgcagaga ggtattttca acatcaatat tttgcgcaaa
    30181 aaagaatgtg gcaacaacag tgataattac acctatcagt aaattcgatt tatttgcttt
    30241 aagcaatgtc caaatcgttt tataaagcgt cataacgcac ccctctttcc atccaagcat
    30301 ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag aagattggta taaaaactag
    30361 ccaaatcagt gtagaaataa agtttataca aactttcgct gatcaagttg acgagattta
    30421 attgaccaac aatcggaata tgaagatcaa tccaatactt cacttgttcc gaccccatca
    30481 taccagcaaa aaagctcatc gcaatcgtca ctgaaatcaa gatgctatcc ttttgcacca
    30541 gattcatttt tggtatcaaa ttaccaacca atgtacctaa caaaatcgca cacaacgacc
    30601 ccagagcggt aacaatcatc aaccattgcc aacgttcacc aaagtctact tgatagataa
    30661 aacgaaagac agctaacata atataaatct ctgtcaaaac aatcgtaaag cttgctgcca
    30721 aattagcgat tgaaactaac gattgctttc taggaatcaa ggacaagcga ataccatttg
    30781 acgattgatt tgcttgctga tcctgtacat tgcggacccc ccacatcgtc ccgtagagaa
    30841 tcgccattcc aatcagagtg aagaaataaa aacttttgaa actcatatta cgacctgcat
    30901 gaacttcttg aaaagatact tcttgttcaa aaacagtcat gatgggcgcg agatcaacgt
    30961 tctccacttc tgccacttta ctcgaaatta taccaatacg ctgtaaaaat tgatccatga
    31021 cgtttttcat caacgtctga ctcattcctg cttgacccac atggagcgaa acagatgaac
    31081 catcaatgag ataaaaacca gccaaatcat ctgcatttag cttttcattc gcttctactt
    31141 ccgatagttc ctgtacttca aataatggtt tatcattgac ttcgacttgt tctaagactg
    31201 tggtaaaatt ttccgaaagc gctgtttgat ccgctttgat tacaattcca gtattaatcg
    31261 tttcgagatt gccaccttga tcaatctcgc caaaggccgc cataaacatc aatcctaaaa
    31321 gaattggaaa ggccaatgtc caaaacaaca tggatttatt tcgcagcagt atttttgtgc
    31381 gatatttata aaaatgccaa aacattattt cccctcctct agtctctcaa atccttgcca
    31441 gtaatctcta aaaagacatc attcaaggac gcttctttgg ttgctatttt tccgtaactg
    31501 atcttttctt cttgtaagat tgtaagaatc ggcaacaata ctgtatttag ttcagtagtt
    31561 gttacttgga gctgaccttt tttataagcg attccatcag aagctattgt tcgctgacaa
    31621 cacgttttct gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc
    31681 ttttagagat tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg
    31741 gtcgcaaaga atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg
    31801 tcgattcagc tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac
    31861 tgtcggctca tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa
    31921 ccgacgtttc aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc
    31981 cactaattga atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa
    32041 accgcaaaaa taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg
    32101 aggaacgacc ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa
    32161 caatttaatg gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga
    32221 tttcccactg ccgtttggtc ctaacagacc taagacttct ccttcttgta cttgcagatc
    32281 aaaatgattc aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat
    32341 catcatgaaa aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa
    32401 gaaactagtg acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta
    32461 tgcataaatc tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc
    32521 tggtaagtca aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca
    32581 ctggtgtcag ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc
    32641 ttttatgtgc ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg
    32701 cccctgaaga aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg
    32761 ttggtaatca tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt
    32821 acttacgaaa aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag
    32881 atactcattg ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac
    32941 aagaaacaca attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc
    33001 atgacaatgt cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga
    33061 tcaatcaaga accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg
    33121 ggatggatcg gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac
    33181 actccttaga gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt
    33241 ttgaaggatt gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa
    33301 ccaacagtat caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg
    33361 ccttttatcg cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg
    33421 gtattggttt gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt
    33481 tccaagaaaa ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat
    33541 aacagtagtc attattgaga tgatccattt gtgacgaaat cgttacaaac cattttagaa
    33601 agcacgaaag agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc gctagtctta
    33661 tacgaaaccc atcgtcccga tgttttgctg acggacatcc gcatgcctaa acaaacgggc
    33721 atcgatgctg ccaaagaaat tttggctaaa tttcccaaag ccatcctttt gcttttgacg
    33781 acattcaaag atgaagaata catccgcgaa gcattttcca tcggtgtcaa agggtattta
    33841 atcaaacaga acttgcaagc gattattcct tctgtcaaat ccgcttataa tggacaggcc
    33901 gtatttggta atgaaatcat cgagactttc actcaaatga tgaagaataa accgacgatt
    33961 gatagtcatg cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa agaagtagct
    34021 gccgggaaaa acaataaaga aattgcagat gccttgtact tgagtgacgg cactgttcgc
    34081 aattacatca gccaattact agaaaagcta gatttacggg atcgcacgca attggctatc
    34141 tatttttatc agcatttgca ataagtctta tctaacacaa aaaaacctga atgatgctcc
    34201 ttcgcatcat tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa ccatttcctc
    34261 acgattgaaa gaatcaagga ttcgcagggc tgccaggatt cgttggattg ttcggcgtgt
    34321 taggcggaaa aataggcgcc ttgtctaacg ttttttctag ttttgattct tcacgatcgt
    34381 ctcgttctac atcaggactt tcattcaatt tgaattcttt ttccatctca tctaactttt
    34441 taaagtcatt gccagattct tttgctaatt tcgctaattt atcttcggtt tctttagcca
    34501 taaagtaacg cccccctttt gatttcagct tagcatatca cactgaaaaa acaaaagaaa
    34561 acgctaacca acgatcattt ttagctattt ctcatcttta aaatcaagca ttttttgccg
    34621 gtataaaatc gtagggagaa ctgttttcca taccaatcat cggatctact aactggttat
    34681 cgatcacttc tgctgtaaga acgaaatctt tagaaacagc aggttctacc tcttctttct
    34741 tttcttctcc aacaaatgaa gaaaacagat ctggttccac tgtttccatt acttggtttt
    34801 tcgtttcatt cgtcccactg tcatcttcta caggtagctt ttcttttaca acccgtggct
    34861 cttttgtata gttcccatct tcaaatggat catttccgac caaagaatct tcataagctg
    34921 ctagtttggt ccggagagtg atattcattt tatcattatc catgatcctt gatttcaacg
    34981 tagtcatccg gcttgctgat tcattcgcta cacaagtgac ataggcttgc tcttccccta
    35041 acaaaatcaa cagttcctta ctgcgagtca ctgccgtgta gagcaaatta cgctgaagca
    35101 tccgctgata ttgatggacc atgggcaaaa taaccatctt gaattcactg ccttgtgatt
    35161 tatggattga gcaacaataa gaaagagtga ttttattcca ttcgtttctt ttataagtga
    35221 cctcatttgc atcaaactgg ataactaatt catccacttt atcttcagaa tcttttgcca
    35281 agatgatacc cgtgatttct cccatatctc cattgaatac attcaattcg ggagtattga
    35341 ctaattggag gactttgtct cctatccgat aaacggtgtc gttccaatgt acttcctttt
    35401 ttccaggttt ccctggattg aatatctcct gcatcatttt atttaacgca tcgatccctg
    35461 caggtccccg atacattggt gctaatactt ggatatcttg tgcacggaat cccttatttt
    35521 tcgctttttc cacaatttta gagatcaagg gttcaatttg ataagcatca cttcggaaat
    35581 aggaacgatc tttttgattc tttgtgaaat cttttggcag acgtccttct ttgatctcat
    35641 gggcaagagg aataatacta gaaccatcac cttgccgata aatctgattg agttccattt
    35701 tgggaatttg ttcgatttcc aaaagatcat ggagtacttg accaggaccg acagagggaa
    35761 gctgatcttt gtctcctact aagataactt gcatattcgt cggaatcgct ttgaataatg
    35821 tgttagccag ccatgtatcg accatagaca tctcatcaac aatcaaaagt ccgccttcta
    35881 attctttcgt actgactgaa ggtgtttttt cccgtccatt cagccccaga agacgatgga
    35941 tcgtactggc aggcagacct gtcgtttcgt tcattcgttt agctgctcgg ccagttggtg
    36001 ctgcaagtag aataggaaaa atttcttggg tatattttga aggatccaat tccagtccgt
    36061 tcaactctgc aaaaagattg acgattccgt tgatcacagt agttttcccc gtacctggtc
    36121 ctcctgttaa aataaataga ggggaacgga ttgcttcagc gatagcttgt tcttgcgaag
    36181 gtccatactg tatgccgaat attttttcga tacgccgaat attcttctgc acttcttttt
    36241 caggataatg gatttctttt ttacgactga gaaggcgctg gatcgaatta ccgatccccc
    36301 attcagaaaa gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt atctttcctt
    36361 cttcaacaag ttggataatt acagtagcaa cctgatcagg ctggatttca attgggcgac
    36421 ttgtttctaa aatacggatc gtttgctgaa gcaatgtctc cgccggtacg tatgtatctc
    36481 ctgtttgaac tgcatgctga aaaatctgat gcaagatagc tgctcgaatt cttctcggtg
    36541 aatctgctgc aataccgatt tgttcggcta gattatctgc acgtttgaac ccgattcctt
    36601 ctatgtcttc gactagctgg tacggatttt cttcgatgat atccaaagac tcgttcttat
    36661 aggcctggta gatactgaaa gctaactgac taccgaaccc gtaacgattc agtcccatga
    36721 tcacttgatc catcccgtgg tttagccgaa tcgtctcagc aagcatttct ctttttggct
    36781 tcgtcaaacc ggagatttgt tttaataccg aaggatcagc aatgattttt tcgatagcat
    36841 cttctcccag caattctaca attttttctg ctgttttctt cccgattcct gggaattttt
    36901 cgctggaaag atacgctacg atcccatttg cagttgttgg cttttcttgc tcatatcttt
    36961 cgagtttaag ctgccttccg tatttaggat gatccactaa ttctccataa aaacgataag
    37021 tctcattttc ttgaatatcg ccgaaacttc cggtgactac aatctctttt tcacggtaat
    37081 cagctgtggt ttcactgatg tgtaccaaaa gcactttgta aaaattacta ggattttgaa
    37141 aaaaaatcgc ggcaactgta ccaacgaaat aaggcatttc ttctggctgc atcggaagcc
    37201 gcctccttct tctatagtaa aggttctggt gctgtttcag ttcctaaaaa atctgtttgg
    37261 ttccatactt tcagttcata tggcaataat ctgcaaggct cttcagtctc taaaatcgtc
    37321 aaactactat tgaacaatcc acccatttca cgcaactcac ttagctcttt tcctgtcatc
    37381 cattgaatgg ctgcagttaa agaagctcca tgtcctacga ataaaagtgg gccatcttta
    37441 tgttgggcaa ccgcatctgc tacaacagtt tcgatacgtg tgattgccga ttcaatcgtt
    37501 tctccgtgaa aaggagaagg atcatacaga tctaaatgat tacgcaaatt cggcagatta
    37561 tccggatatt tctggtacat ttcatcgatc gactgacctt ctaaagtacc cagccctagt
    37621 tctcttagct tatctgtata aaaaatctct accggacgag caagctcttt gttgatacct
    37681 tctgctgtat cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa aggaacatat
    37741 tttacctttt tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag cggagaatcg
    37801 ccattgctcc cttgaaaacg tctttcctga ttccattgtg tcttcccatg tcgggtaaaa
    37861 tatagttcca tacgcagcaa actcctgtct ttcttttatt ttactctata ataaactgtc
    37921 taatccagct tgtcattttc atagtgatgc gtcaattcta aattagggaa tccttgctgg
    37981 cgaagtgctt catacactat cattgcagct gtattcgata aattcaatga gcggacatgc
    38041 tcatcattca tagggatgcg taaacacttt tcttcgtttt cacgcataaa tgtttctggc
    38101 aaaccggttg tttcttttcc aaataaaaag aagtgctctt ctccatcagc aaaattttcg
    38161 tcactatata ctttatgggc aaattttgag atcaaatgta gtttacgatt acctaaaaat
    38221 gttaaaaaag cttctaaatt actgtggtaa gtaatattta catcattcca ataatccaac
    38281 cctgcccgtt ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat taaatgaagc
    38341 ggagtattcg tggctgcaca cgtacgggca atatttccag tgtttgcagg gattaatggt
    38401 tcaaataaaa cgatatgatt catcaggatc attcctctca tatttatgtt cgtttctatt
    38461 ttctgacaat ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca gtcactgcga
    38521 gtcgttacgt tagtgaagct cttctcacta acttttatca gaacaggaac ctgataaaaa
    38581 ccaatgaaaa aacaacttgt tgctaatata acaccctcaa cttttttttg caacctctac
    38641 tcattaaaaa ctgctagaaa aacgttttct cttagttcct tttaataatt ctatgtcaat
    38701 tgtaatcttc ttcaaaggat tcttatataa gtctgctttg attcgctctt ctacctgcca
    38761 ttctaaaggt gacatggcca aaagatcttt ttgattttcc tctggtatct caaattcata
    38821 ggttacacgc tgacgttcag ttaatttcat tgcttcagca aacttggcta ctacacgttc
    38881 attggaataa ctctgttttt caggttgatt cggataaaaa gcctgacgta gttcctttaa
    38941 ataattttct tgtggcacca ccttcaacac ttctccatct tctgagagta cacgttggaa
    39001 ttcttggtaa tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt gatcagcaaa
    39061 cggcaaattg gtcaaatcag ctacacacca aaatgcttcg gcagattgtt ctgtagctgc
    39121 gtagactcct tcttttgaaa tatcaaatcc aacagcattt tgaatctttt tcaattttga
    39181 aagttcattc aagaagctac cttctccgca gccaacatct aatagcgaga cttcttcagg
    39241 taaatgctgc gcaatagtct caataacggg ctgatacatg ccagattgga tcattctacg
    39301 ccttggaacg aacattcctt gatcgtattc tgttttaacc gcatgggata aaaaataaag
    39361 tgttcctttt ttggaaaggt caaaacggtg attatttttg catatcaagc cattatcaga
    39421 cttttttact tcttcttggc aaaccggaca gcggaacagc tgatgatttt ctgccaaaaa
    39481 ctggcgtcct cggtcaattt tttttaacat agttccccct catttctgtt ttatcttacc
    39541 gttagatgct cactttggca actgctaaaa atatgataca atgcctagaa gaaagtgaaa
    39601 gataaaggag aagattagat gatcaaagaa ttttgcgctg aaaactacac atctattcct
    39661 ctagcaattg ctagaggagc cgatcggatc gaattgtgtg acaacttagc ggtcggtggc
    39721 acaaccccga gtactggtgt gatcgaagaa acgttaagtt atgctgggga aaaagatatc
    39781 cccgtaatga cgattattcg tcctagaggg ggcgacttcg tctataacga tatcgaattg
    39841 aaaatcatgc atactgattt gattgaagca aagaaattag ggacggatgg cgtagtattt
    39901 ggttgtctta ctccttcagg ctggctagac gaagaggcct tagaactatt gatcgaaaca
    39961 gcagaagggt tgcagatcac cttccatatg gcttttgaca gtattcctga agatcggcaa
    40021 ttcgaagcaa tcgattggct ggtagaacat ggtgttgacc gtatcttgac tcatggcggt
    40081 cctgctgaca aaactattgt agaaaatttc ccacggctta aagaattgat tgcctataca
    40141 gatggccgct taatcatttt gcctggtggt ggtgtcactg cagagaatgc agcacatgta
    40201 gctaaagaat tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct tcagttataa
    40261 tcttataaac aaaaaaacat ttgcaacagg cataatgctt attgcaaatg tttttgtttt
    40321 tttacataaa atagcccata aataaagaag caatattgaa ataaatcagt acactcgtca
    40381 aatcgctcaa agtcgtgata aagggaccgc ttgctactgc tggatcaaat cctagacgat
    40441 ccattagcat cgggattaaa ctacctgcaa gattcgccac tgtgattgca aatagcatag
    40501 ccatccctat gacaaacccc aagataaaat tatgtttcca aaaacctacg attagaaaaa
    40561 tcgtgatccc cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc aaaacaagtc
    40621 gaacaaatcc atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc acagctagcg
    40681 actgggttcc agcattaccg gcagttcctg taatcagcga aatgaagact gctaaaatac
    40741 tcgcttcact taccaagtct tcatagtgac tgattaaaga agcggtcgac atacctaaaa
    40801 acaacagagt gatcagccaa ggcaaacgtc gagacgctgc ctttactgga ttttcattaa
    40861 tttcttccac attgaccccg gcaagtcctg aataatcgct agcagcttca tcatcaataa
    40921 cgtcgatgat atcatcgact gtgacgattc ctaacaaatg atcatcataa tcggtaacag
    40981 gaagagctaa gaagtcataa tctctaaatg tttgtgccac atcttcttgg tcgtcgccta
    41041 catgaactga aagcactcgt tcgctcatca gatcagaaat cattgcgtcg tcatcattca
    41101 cgattaggtc gcgcagagaa ataaccccta ctagctgacc ttcttgatta acgacataga
    41161 tgtaatagat tgtttcagct acatctgctt cgttcttcag cacgtacatc gcagagcgaa
    41221 cagtttgatt ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca gccgtttcat
    41281 cctcgtaatg gagaagttcc ttgatttcac tggcatcgtc tgccgacatc aaacttaaat
    41341 atttagcaat ttgcttttta tccagcgtat taagcaagtc aacagcatta tctgtataca
    41401 tctcagaaag catatcagct gcatagctag gacgcatttc atctaggtat tctttcatat
    41461 gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa ttctttaggc gacagatagg
    41521 aatagatcag ctgcctgttc tcttctgtaa gagattggta gaattgcccc tgctcataga
    41581 tatgcatctc taaaaaactg tttctgaatg cctgaatatt attttccctt aaatcttgag
    41641 tcaactgggc aaatctttcc tctaattctt gattttcatc caaaccaatc tcacctcgcc
    41701 ttattcttca aaccgtctca caactgcctc catgtctgcg gctaaaggct gcttgaactc
    41761 cagcaactct tctgtaaatg gatggtaaaa acgcagatca taacaatgaa gggcttgtct
    41821 ttttattccc agatccattc gcccgccata catatcatca ccgagcaggg cacagccaat
    41881 tgcggaaaaa tgaactctta tttgatgtgt ccttcctgta tgcagctgga tatcaactaa
    41941 cgcgatttct gttagtcgct tctctatcca gtactcagtc tgtgccgaac gtccttcatc
    42001 agagaccatt cgcttcaata gcgaagaata atctcgggcg atcggcaaat cgatatgtcc
    42061 atgtgccgat aaatctgaaa gccttccact gactaaggcc tgatattttt tcacaaactt
    42121 cttttcacga agttgcatat ctaattttgc gtgcgcaaat ccatgctttg caaaaagcat
    42181 caaaccagat gtatcccgat ctagtcgcgt caccacatgg attacttggt tttcgtaacc
    42241 ttgtttttta taataagcct ttacacgatt agccattgtc ccatttggat ggtattgggc
    42301 aggaatagac gaaacgcctg ctggtttatt tactaccaga acgtgttcat cttcatacac
    42361 gatatcgatc ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag caggaatcac
    42421 aatggttacc ttatcatcct tagctagaga aaacaagaca ttttctactt gatcattaac
    42481 tttgatttga ccgccttgaa attttatttt cgctaacaga ccctttgaga tccctttttc
    42541 ttttaaaaaa tattttactt gctgagaatg atctttctca taatcccatg taaactgcat
    42601 atcaatcctc actaataaag gcatctttca cacgatgcca aaaatgcata tgtcgataag
    42661 aagcaaaatg gatccgttct tctgctattc gataataaat tgagcggata tttgcttttg
    42721 cgacattcaa ttgatcaacc gttacaagat aatcttctgt attttccaat tttatttcta
    42781 gccattcatc atgggcaatc acgatcggag aacccaaagt acgaaacact cgattattca
    42841 gtgaagcaat ctctgccaac tgaaaggcat tgatactagg atggatcaca gcaccgccga
    42901 cacttttatt atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca tctcctctaa
    42961 aactttcaaa cagctcgttt ttgatgtata catcggctac catcgtacga ttggcccgtt
    43021 taatcgttga ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc aaataactga
    43081 tccgaacatc cagcaaggga taactaacac tttgttcccg gttcgttcgt aagctgtcta
    43141 ctagttcttc cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga cctgtatgca
    43201 cgcccaagaa acgcacatca ttcagacaat gactgtagtg atggaacgcc gacaataatg
    43261 tgccgtctcc gccgactgag atgaccaatt ctggctcatg atcatctcgt tgaatccctg
    43321 cttgttctaa aagtgcgttc aaacgttctg tcacttctat agtctttgct tctttattat
    43381 gaacgatcgc tactttcatc ccctgcctcc tctctttcgc tttctttctt caaaagcgtt
    43441 cgatagtaga catcatctga ttttcctctt ccatgggaga ataaccgctg tgcttcttgg
    43501 atttcttcac ggatactgga catctcttca tccaactgat aggcagcttc tgccgcacgc
    43561 tgaagtcgtt cactcatttc ttctggaaaa acgccttgat atttataatt cagcgaatgc
    43621 tcaatcgttg cccagaaatt catcgcaaga gtccgaatct ggatttccgc caaaattttc
    43681 ttctcaccgc tgatcagctg tacaggatac tcgatcacca gatgataaga acggtagcca
    43741 cttgcttttt tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc ttttctttta
    43801 cggatgatct caacaacttg atggatatct tctacaaact gacacatgat ccttaacccg
    43861 gcaatatcag acatttcttc ttctaaacgt cccattggaa tatgccgtaa ttgtgatttt
    43921 gttacaatac tatcgactgg cttgactctc ccagtgacaa attcaatcgg gacatgtcga
    43981 ttttgttcac ggaactgttt gcggatcccc cgcagtttca ctttcaattc tgatacagtt
    44041 tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac ctctacttca
    44101 ttaacattca ttgtttattc taccatatat gcaaagtttt ttcgtactct tgatttatgc
    44161 attgaccaaa aaaagaaaat taggcaaaat agacaaaaag gagtcgatca aatgacacaa
    44221 atgcttgaaa tcgaatataa atctatgctg acaaagcaag agtacgagat attcatcgct
    44281 cactatcaat taacagataa agatttccgt gtacagacta atatttattt cgataccgtt
    44341 gatgaacagc tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg tcatcaagct
    44401 gaatatacat taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga tacctttata
    44461 acagaagaag cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg cgcagttttt
    44521 caaaaacttt ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg aaagcttaca
    44581 acaaaacggg ctgaattccc tattgaagaa ggacttttag ctattgatga aagctgggga
    44641 gacaatctac acgattttga actggaacta gaggtaaggg gatgcatctg tgggaaaaat
    44701 tgcttttata aattttttaa aacgtttttc tttaccttat cgtcctgcta agaacaagat
    44761 ccaacgtatg cttgaagcta aaaattagaa acctttcatt ttcacaaggc acttgagttc
    44821 atttttttac ttttttctgt taagttatag gatgtgaaag aatctagtca ctagttgtat
    44881 agaatctatt ttgggggaga ttcatatgat cgaaatctat ttatttgtta atcctttagg
    44941 ggaatactgt ttcgaaatgg aacaacagtt attacaattt atcgaagaag agtacgggac
    45001 accatcaaaa gaaaagatgc aatttcgctt tcttcctttg gtcaatttgc agacaattgg
    45061 ggatgtcatg caacaaaaag gaatttctca gaacaatctt gaggaacgta atcacttatt
    45121 ctcaatgact tattctgcag cattggactg caaagcggcg cagtttcaag gaaagaaaaa
    45181 aggccgccgt tttttgatca aattgcaaga agcagtcggc tgtaatgaca taccatattc
    45241 tcaagaactt gctgaatcca tatttgaaga aatcggcggc gatttgaata tgtttaaaga
    45301 agatcgtcag tctgacttcg ttaaagaagt attcttttct gatcaaaaaa ttgctcggga
    45361 aatgggcatc acaaaacacc cttctgcagt cgtttacaat tatgcctgcg accgtgattt
    45421 tggtgtttta gtagaagtaa aaggtacaag tagcctttgg agaatcacag agctgtgtaa
    45481 aacaacagat aactcatatc aagtttttaa tggtgaacat ttacatacaa atgattctca
    45541 gttccattca agaactagcc atttacactt gctttctaat taaaaaaata cgtcgactcc
    45601 cttacaagag agccgacgta tttttttcgt ttctattcga gaaaatcaaa aatagaaagc
    45661 cttataaacg atcgatcaat tcttctaatt catttagacg ttgttcaaac atcgacattg
    45721 catcttcaat atatgcagcc tgtgtcatat caactcctgc ttttttcatt acttccacag
    45781 gataatcgct gttacctgct tttaggtaag ccaagtagtt ctctagtgcc tctggttcct
    45841 ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa gccagtcgag tattggaaaa
    45901 catagtaatt ataataaaaa tgcggtatcc gtgaccattc gaatttaatt tctgggtctt
    45961 cttcgactgc tggaccataa tattttgcat tcaatttacc ataactatca cttaggtatt
    46021 cactagtcaa tggcacacct ttttcatctt ccgtatgcat gaaatgctcg aattcagcga
    46081 attgtgtctg gcggaaaact gttcctttaa acccatccaa gtaatggttg agtacataag
    46141 cccgtacacg agggtctttt tctgtttcca ataaatactc cgttaggata ttttcattcg
    46201 ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc gtacacataa ggctggttcg
    46261 aacgagtgaa ataactatga acactatgtc ccatttcgtg gacaagcgta aatagctgat
    46321 ccagcgtatc atgccaattc aataaaatat atggatttgt gtcatagctt cccgaagaat
    46381 aagcaccgct tcgtttccct ttattttcga caacatcgat ccaacgttca gagaatgctt
    46441 tttctacgat ggccatgtat tcttcaccca ttggtttcag tgcttctaaa gctttttctt
    46501 ttgcttcttc gtacgtaaag gtaattggag cttcccctaa gaccggtgta taaagatcat
    46561 acatgtgcag tttttccact tctaataaac gtttccgtaa ttccatgtat cgatgtaaca
    46621 aaggcaaatg cttgtttacc acgtctacca aagtatcgta tacactttca ggaatatgat
    46681 tattgctcaa agacgcttct ctggcagagc tgtaattacg gactttcgct ttaaaattat
    46741 gtccttttat atgtgtgcct aaagtagaag caaatgtatt tctaaattgt tcgtaaacac
    46801 tgtacaatcc cttaaatgct gcttcgcgca cccttcgatc cgtgctttct agcaactgac
    46861 catacacgcc atgagataat tggactattt caccattttc tccttcaatc gttggaaaaa
    46921 ctagatctgc attattcaaa acagcaaatg tatcacttga agcatcaaag atttcacctg
    46981 ctccagcaag aagagattcc tgctcagcag ataagacatg ggctcgatta tctactattt
    47041 gctggatata atggcgatag acttccaatt ttggttcttc tttgaaatat tgccaaatct
    47101 ggtcatctga caattgcaat atttctggtt caaaccagga aacggcttca ctaactttcg
    47161 aaaacagact gcttgctctt gcataaagcg cttggtaatc tgtatttcca gtgtcttgat
    47221 cgtttttaag gtgcgcatat acataaatga cttcagtttg gcgataaacc ctcaatacga
    47281 attcaatcgc atttaaaaat tgagaagcgc cttgatcaag tgttcctttg tgtttttcag
    47341 attgttttaa ctcttctgat aattccaaat atttctcatc aaactcttgg tcgcttgaaa
    47401 agatcttggt caagtcccaa gttaaatttt caggcaattc ttctcgtttt ggcaactgct
    47461 ttacttccat gaaatccact ccttttcttc ccttagctct atctatcaaa atagatcata
    47521 gtctaacatc cacatgtcac agcatctaac ttttctactc attcatgtgt ctgcttccat
    47581 caattttagc ataactaaat cttgttttac atagaaaacc gattcctagc ctatttttat
    47641 acttactatt ttttcaaaga agacaagtgt atgcattcgt taaaaaactg ctgatagacc
    47701 atctcttcat caatcatagg aaaaagccat tcccttttat gttcttttat ttggcaaagc
    47761 catgagttga aatcaggaac tttctggttt tccaaggctt cttcgtacaa cattcgataa
    47821 agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt tttgatagat ccacgatgat
    47881 agatgcaaaa gatgtttatg acgcaagtag cattgttctt gtatggatac tgtattcttt
    47941 tgcttgtgaa taagttggcg tgtcagccat ttcttcccat cttcactcac tttttctatt
    48001 tttttcattt ggttcatttt aatttcgtta ctagtgaata tttctttaag gtccctacta
    48061 gaaaaagtcc agcagctact gcaaaaggaa atatgccctg atacagtctc tgtaatatga
    48121 tgatacaaat aaagcttttg tttcttgaga tcagctttcc agcaatgcac tcctcttttt
    48181 ctattatagt agcagcaact tttttctatc aatgagaatt gcgattgtcc aagaaaagaa
    48241 ttaccaagta tccaccatac ttggtaactg ttattcaaat aattttgggt tctttcaata
    48301 aatctctggt gagacagtct agaacattgg atttctattg ctagattcgc caacaaaata
    48361 tccggccttt gccgcaacct cggcaaatag gcttccaaaa atactggttc tactgactgt
    48421 cccagccaat ccatgaagca ttcctttaac tgtaagtgct cttttgattc tctttcacta
    48481 aagaccgaac agttatctga tttttgatgg gcaaaatgag cacatttttt ctttcccttt
    48541 ttcaaaatca ctctttttcg acagccacga caataatatt tctttccttt ttctgcatgt
    48601 ttagcaaaac agcacttatt atattcattc attgcaagca gcatttttct ccctctgaac
    48661 ttatttacgt ctaagaattt atttacacga aaaaagaggg cgtgaaaaag ctattctgca
    48721 tcaagtaata agaacagcta aacaaaaata gttcccaata tttttcgttt ggttgggctt
    48781 gttaccgcag atgcaagctt ttgaacacat ttatacgaaa aaaagactgc gacacaattt
    48841 ttgtcacagt ctcttactat aatgctgatt tatacaaaat aaaatttcgt tcgctcgata
    48901 gcattacgcg ccatcagaca ttgtccatat tcattcaatg tttctggtgt acgatcgctc
    48961 ttttcagcaa actccaaaat tcgagcaatc tgattctcta catccgcttg gttgagattt
    49021 tccatccaga agtgaacgac taggtaatag cgctcttcga ataaatacaa atcagtccaa
    49081 gcagactcta gttcgacatc tgttgcaagt tggatcactt gttcaaaatc ttcaaaagca
    49141 aaagttgctt gtctttcctg ttcttctagg gcgcttccag ataattcttc ctccggatcg
    49201 ctgccaataa tctgctgacg aagaaagtca ctcacatctt ctgaatcaac agtagaatct
    49261 tctgattgag aaatttcttc atccggaaga tttttactga taaacagttc taatccgtct
    49321 ccttttggca aaacttggaa agtgacagct tcgcttcctc ggaactcctc ttcgatatca
    49381 acttcttcca aaatactata aaagaaattt tcgatttctt tgtgattgcc aagtaaatcc
    49441 aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa ttagtacacg gatcgtattt
    49501 tcattaatat gttccatttc cattttgcta cacctcactt tgccacaatg tttaatatag
    49561 attcattgta gcgaaatcag cttaaatgta aagaaaaaaa atctcgctgt taagcgaatt
    49621 taataaaacc agcggatttt tcaacctttc aagctatctt atttaactat aacatttttg
    49681 gcagacgaat actatatagc cgacttttca gctaattttc cctctctatt tttcgtgcaa
    49741 tcatcatctt ctactacctt tttatacatc taatcaagaa tactttttgt aaaaacaaaa
    49801 aaacttggaa aaactatctt tgtaatagt
//
LOCUS       DASJRQ010000009        44860 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJRQ010000009 DASJRQ010000000
VERSION     DASJRQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.009"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            212..604
                     /locus_tag="U4A80_000477"
     CDS             212..604
                     /locus_tag="U4A80_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1974145.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="U4A80_000478"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="U4A80_000478"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1974146.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="U4A80_000479"
     CDS             3165..4775
                     /locus_tag="U4A80_000479"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1974147.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="U4A80_000480"
     CDS             5067..5936
                     /locus_tag="U4A80_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1974148.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="U4A80_000481"
     CDS             6101..7372
                     /locus_tag="U4A80_000481"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1974149.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="U4A80_000482"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="U4A80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1974150.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="U4A80_000483"
     CDS             8763..9026
                     /locus_tag="U4A80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1974151.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="U4A80_000484"
     CDS             9471..9920
                     /locus_tag="U4A80_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1974152.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="U4A80_000485"
     CDS             9977..11395
                     /locus_tag="U4A80_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1974153.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="U4A80_000486"
     CDS             11395..12255
                     /locus_tag="U4A80_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1974154.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="U4A80_000487"
     CDS             12429..13202
                     /locus_tag="U4A80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1974155.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="U4A80_000488"
     CDS             13349..13714
                     /locus_tag="U4A80_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1974156.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="U4A80_000489"
     CDS             complement(13862..15070)
                     /locus_tag="U4A80_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1974157.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="U4A80_000490"
     CDS             complement(15089..15367)
                     /locus_tag="U4A80_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974158.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="U4A80_000491"
     CDS             15521..16216
                     /locus_tag="U4A80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1974159.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="U4A80_000492"
     CDS             complement(16658..17455)
                     /locus_tag="U4A80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1974160.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="U4A80_000493"
     CDS             complement(17452..18636)
                     /locus_tag="U4A80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1974161.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="U4A80_000494"
     CDS             complement(18661..19908)
                     /locus_tag="U4A80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1974162.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="U4A80_000495"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="U4A80_000495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1974163.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="U4A80_000496"
     CDS             21888..22202
                     /locus_tag="U4A80_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1974164.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="U4A80_000497"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="U4A80_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1974165.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="U4A80_000498"
     CDS             22887..23531
                     /locus_tag="U4A80_000498"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1974166.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="U4A80_000499"
     CDS             23546..23875
                     /locus_tag="U4A80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1974167.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="U4A80_000500"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="U4A80_000500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1974168.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="U4A80_000501"
     CDS             24863..25687
                     /locus_tag="U4A80_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1974169.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="U4A80_000502"
     CDS             25680..26027
                     /locus_tag="U4A80_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1974170.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="U4A80_000503"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="U4A80_000503"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1974171.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="U4A80_000504"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="U4A80_000504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1974172.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U4A80_000505"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="U4A80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1974173.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U4A80_000506"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="U4A80_000506"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1974174.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="U4A80_000507"
     CDS             31657..32451
                     /locus_tag="U4A80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974175.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="U4A80_000508"
     CDS             32441..34483
                     /locus_tag="U4A80_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974176.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="U4A80_000509"
     CDS             34764..34913
                     /locus_tag="U4A80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1974177.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="U4A80_000510"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="U4A80_000510"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1974178.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="U4A80_000511"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="U4A80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1974179.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="U4A80_000512"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="U4A80_000512"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1974180.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="U4A80_000513"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="U4A80_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1974181.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="U4A80_000514"
     CDS             39330..40412
                     /locus_tag="U4A80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1974182.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="U4A80_000515"
     CDS             40412..41215
                     /locus_tag="U4A80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1974183.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="U4A80_000516"
     CDS             41285..42025
                     /locus_tag="U4A80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1974184.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="U4A80_000517"
     CDS             42036..43040
                     /locus_tag="U4A80_000517"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1974185.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="U4A80_000518"
     CDS             complement(43085..44782)
                     /locus_tag="U4A80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974186.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKFYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14422 a         7826 c         9341 g        13271 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa atttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat
//
LOCUS       DASJRQ010000010        43297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJRQ010000010 DASJRQ010000000
VERSION     DASJRQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.010"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            252..1163
                     /locus_tag="U4A80_000519"
     CDS             252..1163
                     /locus_tag="U4A80_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1974187.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1541..2458)
                     /locus_tag="U4A80_000520"
     CDS             complement(1541..2458)
                     /locus_tag="U4A80_000520"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1974188.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2660..3097
                     /gene="zapA"
                     /locus_tag="U4A80_000521"
     CDS             2660..3097
                     /gene="zapA"
                     /locus_tag="U4A80_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1974189.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3155..3703
                     /locus_tag="U4A80_000522"
     CDS             3155..3703
                     /locus_tag="U4A80_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN1974190.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3818..6178
                     /locus_tag="U4A80_000523"
     CDS             3818..6178
                     /locus_tag="U4A80_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1974191.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6265..6579
                     /gene="trxA"
                     /locus_tag="U4A80_000524"
     CDS             6265..6579
                     /gene="trxA"
                     /locus_tag="U4A80_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1974192.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6761..8539
                     /gene="uvrC"
                     /locus_tag="U4A80_000525"
     CDS             6761..8539
                     /gene="uvrC"
                     /locus_tag="U4A80_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1974193.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8684..9583
                     /locus_tag="U4A80_000526"
     CDS             8684..9583
                     /locus_tag="U4A80_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974194.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9576..10838
                     /locus_tag="U4A80_000527"
     CDS             9576..10838
                     /locus_tag="U4A80_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974195.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10999..12897
                     /locus_tag="U4A80_000528"
     CDS             10999..12897
                     /locus_tag="U4A80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN1974196.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13227..13946)
                     /locus_tag="U4A80_000529"
     CDS             complement(13227..13946)
                     /locus_tag="U4A80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1974197.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14283..14615
                     /locus_tag="U4A80_000530"
     CDS             14283..14615
                     /locus_tag="U4A80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974198.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14602..16605
                     /locus_tag="U4A80_000531"
     CDS             14602..16605
                     /locus_tag="U4A80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1974199.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16624..17094
                     /locus_tag="U4A80_000532"
     CDS             16624..17094
                     /locus_tag="U4A80_000532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1974200.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17115..17699
                     /locus_tag="U4A80_000533"
     CDS             17115..17699
                     /locus_tag="U4A80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1974201.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17714..18700
                     /locus_tag="U4A80_000534"
     CDS             17714..18700
                     /locus_tag="U4A80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1974202.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18690..19001
                     /locus_tag="U4A80_000535"
     CDS             18690..19001
                     /locus_tag="U4A80_000535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1974203.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            19023..20804
                     /locus_tag="U4A80_000536"
     CDS             19023..20804
                     /locus_tag="U4A80_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1974204.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20797..22173
                     /locus_tag="U4A80_000537"
     CDS             20797..22173
                     /locus_tag="U4A80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1974205.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22180..22815
                     /locus_tag="U4A80_000538"
     CDS             22180..22815
                     /locus_tag="U4A80_000538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1974206.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22845..24200
                     /locus_tag="U4A80_000539"
     CDS             22845..24200
                     /locus_tag="U4A80_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1974207.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24392..25786)
                     /locus_tag="U4A80_000540"
     CDS             complement(24392..25786)
                     /locus_tag="U4A80_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1974208.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25878..26849
                     /locus_tag="U4A80_000541"
     CDS             25878..26849
                     /locus_tag="U4A80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1974209.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26846..27628
                     /locus_tag="U4A80_000542"
     CDS             26846..27628
                     /locus_tag="U4A80_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1974210.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27797..28168)
                     /locus_tag="U4A80_000543"
     CDS             complement(27797..28168)
                     /locus_tag="U4A80_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1974211.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28302..28745
                     /locus_tag="U4A80_000544"
     CDS             28302..28745
                     /locus_tag="U4A80_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1974212.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28788..29597)
                     /gene="yidA"
                     /locus_tag="U4A80_000545"
     CDS             complement(28788..29597)
                     /gene="yidA"
                     /locus_tag="U4A80_000545"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1974213.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29616..30983)
                     /locus_tag="U4A80_000546"
     CDS             complement(29616..30983)
                     /locus_tag="U4A80_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1974214.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30967..31797)
                     /locus_tag="U4A80_000547"
     CDS             complement(30967..31797)
                     /locus_tag="U4A80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1974215.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31960..32397
                     /locus_tag="U4A80_000548"
     CDS             31960..32397
                     /locus_tag="U4A80_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1974216.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32473..33093
                     /locus_tag="U4A80_000549"
     CDS             32473..33093
                     /locus_tag="U4A80_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1974217.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33282..33788
                     /locus_tag="U4A80_000550"
     CDS             33282..33788
                     /locus_tag="U4A80_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1974218.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33805..34704
                     /locus_tag="U4A80_000551"
     CDS             33805..34704
                     /locus_tag="U4A80_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1974219.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34791..35675
                     /locus_tag="U4A80_000552"
     CDS             34791..35675
                     /locus_tag="U4A80_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1974220.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(35867..35938)
                     /locus_tag="U4A80_000553"
     tRNA            complement(35867..35938)
                     /locus_tag="U4A80_000553"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35903..35905),aa:Arg,seq:ccg)
     gene            35998..37332
                     /gene="rpoN"
                     /locus_tag="U4A80_000554"
     CDS             35998..37332
                     /gene="rpoN"
                     /locus_tag="U4A80_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1974221.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            37481..38572
                     /locus_tag="U4A80_000555"
     CDS             37481..38572
                     /locus_tag="U4A80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1974222.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            38613..39614
                     /gene="gap"
                     /locus_tag="U4A80_000556"
     CDS             38613..39614
                     /gene="gap"
                     /locus_tag="U4A80_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1974223.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            39724..40914
                     /locus_tag="U4A80_000557"
     CDS             39724..40914
                     /locus_tag="U4A80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1974224.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            41018..41773
                     /locus_tag="U4A80_000558"
     CDS             41018..41773
                     /locus_tag="U4A80_000558"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1974225.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            41889..43187
                     /gene="eno"
                     /locus_tag="U4A80_000559"
     CDS             41889..43187
                     /gene="eno"
                     /locus_tag="U4A80_000559"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1974226.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        14344 a         7538 c         9229 g        12186 t
ORIGIN      
        1 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
       61 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
      121 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
      181 cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt
      241 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
      301 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
      361 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
      421 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
      481 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
      541 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
      601 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
      661 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
      721 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
      781 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
      841 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
      901 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
      961 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
     1021 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
     1081 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
     1141 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
     1201 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt ttaaggaaga
     1261 ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa acagcttctc
     1321 atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac cgtttaagaa
     1381 aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga aaaatagttc
     1441 ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac accgtttatt
     1501 cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt agtagcttca
     1561 aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata ggcatgccgc
     1621 ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct gacggaaggg
     1681 tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca cgactgatga
     1741 tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag tacagttttt
     1801 cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc ggcgtaaact
     1861 gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc aaatggatcg
     1921 cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg atctcattgt
     1981 atttttctgg agataagaca agcagtttat aggggatggt tttttttaag atgtcagcaa
     2041 gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt ttacgtaatt
     2101 ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg aaatagctgc
     2161 catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt ggcaaagaag
     2221 aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta tcagcttcta
     2281 ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta atggtcgtcc
     2341 cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa taagagcgat
     2401 agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg ttgctcataa
     2461 aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc gttttctaag
     2521 agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt atgtgaaaaa
     2581 tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa ctttcatcaa
     2641 ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg tgattgccaa
     2701 ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg taacgaagct
     2761 gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata atgaacaagc
     2821 agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag aaagaatatt
     2881 ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta cagagcttga
     2941 aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat tgaaagaaaa
     3001 tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt taaacgagaa
     3061 acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca aaataagaaa
     3121 aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt
     3181 attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc
     3241 gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca
     3301 tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag cattttttga
     3361 tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat
     3421 tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta
     3481 tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc
     3541 ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca
     3601 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
     3661 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
     3721 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
     3781 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
     3841 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
     3901 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
     3961 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaaat
     4021 tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga
     4081 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
     4141 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
     4201 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
     4261 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
     4321 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
     4381 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
     4441 tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg aaccaaaaca
     4501 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
     4561 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
     4621 tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
     4681 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
     4741 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
     4801 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
     4861 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
     4921 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
     4981 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
     5041 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
     5101 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
     5161 ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga
     5221 gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
     5281 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
     5341 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
     5401 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
     5461 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
     5521 ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa
     5581 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
     5641 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
     5701 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
     5761 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
     5821 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
     5881 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
     5941 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
     6001 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
     6061 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
     6121 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
     6181 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag
     6241 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
     6301 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
     6361 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
     6421 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
     6481 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
     6541 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
     6601 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
     6661 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
     6721 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
     6781 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
     6841 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
     6901 tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
     6961 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
     7021 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
     7081 aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg
     7141 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
     7201 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
     7261 atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
     7321 agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
     7381 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
     7441 tgccattgag acaatcatga cttgccaaaa aatgacaaat actgatttac tggatcgaga
     7501 tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
     7561 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
     7621 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
     7681 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
     7741 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt
     7801 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
     7861 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
     7921 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
     7981 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
     8041 atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt
     8101 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
     8161 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg ataaacataa
     8221 aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc
     8281 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
     8341 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg
     8401 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
     8461 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
     8521 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
     8581 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
     8641 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
     8701 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
     8761 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
     8821 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
     8881 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
     8941 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
     9001 tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
     9061 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
     9121 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
     9181 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
     9241 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
     9301 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
     9361 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
     9421 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
     9481 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
     9541 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
     9601 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
     9661 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
     9721 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
     9781 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
     9841 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
     9901 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
     9961 gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct
    10021 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    10081 cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct
    10141 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    10201 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    10261 aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    10321 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    10381 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    10441 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    10501 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga
    10561 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    10621 tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg ggtggagcat
    10681 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    10741 tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat
    10801 cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg
    10861 aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat ttattttact
    10921 atattggata tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca
    10981 atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca
    11041 acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga
    11101 acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt
    11161 atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa
    11221 acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat
    11281 tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt
    11341 acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat
    11401 atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa
    11461 aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca
    11521 cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga
    11581 ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa
    11641 tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg
    11701 acacttttac ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg
    11761 ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt
    11821 gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat
    11881 caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa
    11941 tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat
    12001 ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat
    12061 ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag
    12121 cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc
    12181 acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc
    12241 ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa
    12301 cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt
    12361 tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt
    12421 gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac
    12481 ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa
    12541 gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc
    12601 aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg
    12661 acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat
    12721 tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa
    12781 aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa
    12841 ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa
    12901 gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct
    12961 ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa
    13021 caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct
    13081 cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    13141 tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
    13201 ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc
    13261 tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta
    13321 tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa agcgatggct
    13381 tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga
    13441 tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat
    13501 tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct
    13561 tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa
    13621 attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga
    13681 atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct
    13741 tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga
    13801 gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct
    13861 tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt
    13921 ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa
    13981 ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
    14041 ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt
    14101 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
    14161 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
    14221 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
    14281 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa
    14341 agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat
    14401 tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac
    14461 aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
    14521 tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca
    14581 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
    14641 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc
    14701 cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc
    14761 acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa
    14821 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
    14881 cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat
    14941 aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat
    15001 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
    15061 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
    15121 agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg
    15181 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa
    15241 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
    15301 aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg
    15361 acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca
    15421 cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc
    15481 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa
    15541 accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag
    15601 caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact
    15661 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt
    15721 tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt
    15781 gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa
    15841 ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc
    15901 ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg
    15961 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg
    16021 gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc
    16081 agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc
    16141 atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc
    16201 ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa
    16261 ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt
    16321 ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct
    16381 ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
    16441 ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc
    16501 gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg
    16561 gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat
    16621 ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg
    16681 gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag
    16741 gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa
    16801 ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac
    16861 ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt
    16921 gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa
    16981 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
    17041 acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa
    17101 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag
    17161 agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac
    17221 aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
    17281 tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt
    17341 tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa
    17401 attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga
    17461 caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt
    17521 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
    17581 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
    17641 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
    17701 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
    17761 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
    17821 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
    17881 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
    17941 ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt
    18001 gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
    18061 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag
    18121 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
    18181 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
    18241 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
    18301 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
    18361 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
    18421 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
    18481 aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
    18541 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
    18601 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga
    18661 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
    18721 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
    18781 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
    18841 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
    18901 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
    18961 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
    19021 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
    19081 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
    19141 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
    19201 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
    19261 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
    19321 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
    19381 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
    19441 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
    19501 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
    19561 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
    19621 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
    19681 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
    19741 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
    19801 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
    19861 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
    19921 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
    19981 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
    20041 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
    20101 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
    20161 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
    20221 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
    20281 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
    20341 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
    20401 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
    20461 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
    20521 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
    20581 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
    20641 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
    20701 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
    20761 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
    20821 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
    20881 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
    20941 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    21001 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    21061 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    21121 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    21181 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    21241 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    21301 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    21361 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    21421 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    21481 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    21541 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    21601 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    21661 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    21721 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    21781 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    21841 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    21901 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    21961 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    22021 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    22081 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    22141 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    22201 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    22261 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    22321 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    22381 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    22441 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    22501 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    22561 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    22621 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    22681 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    22741 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    22801 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    22861 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    22921 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    22981 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    23041 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    23101 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    23161 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    23221 tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg
    23281 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    23341 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    23401 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    23461 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    23521 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    23581 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    23641 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    23701 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    23761 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    23821 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    23881 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    23941 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    24001 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    24061 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    24121 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    24181 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    24241 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    24301 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    24361 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    24421 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    24481 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    24541 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    24601 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    24661 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    24721 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    24781 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    24841 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    24901 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    24961 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    25021 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    25081 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    25141 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    25201 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    25261 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    25321 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    25381 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    25441 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    25501 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    25561 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    25621 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    25681 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    25741 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    25801 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    25861 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    25921 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    25981 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    26041 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    26101 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    26161 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    26221 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    26281 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    26341 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    26401 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    26461 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    26521 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    26581 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    26641 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    26701 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    26761 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    26821 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    26881 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    26941 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    27001 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    27061 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    27121 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    27181 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    27241 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    27301 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    27361 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    27421 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    27481 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    27541 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    27601 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    27661 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    27721 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    27781 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    27841 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag
    27901 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    27961 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    28021 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    28081 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    28141 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    28201 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    28261 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    28321 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    28381 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    28441 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    28501 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    28561 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    28621 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    28681 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    28741 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    28801 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    28861 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    28921 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    28981 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    29041 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    29101 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    29161 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    29221 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    29281 tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg tgtcagtccg
    29341 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    29401 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    29461 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    29521 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    29581 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    29641 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    29701 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    29761 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    29821 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    29881 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    29941 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    30001 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    30061 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    30121 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    30181 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    30241 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    30301 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    30361 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    30421 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    30481 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    30541 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    30601 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    30661 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    30721 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    30781 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    30841 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    30901 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    30961 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    31021 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    31081 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    31141 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    31201 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    31261 gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    31321 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    31381 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    31441 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    31501 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    31561 acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga tcccgtcact
    31621 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    31681 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    31741 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    31801 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    31861 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    31921 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    31981 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    32041 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    32101 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    32161 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    32221 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    32281 ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg
    32341 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    32401 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    32461 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    32521 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    32581 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    32641 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    32701 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    32761 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    32821 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    32881 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    32941 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    33001 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    33061 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    33121 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    33181 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    33241 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    33301 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    33361 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    33421 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    33481 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    33541 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    33601 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    33661 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    33721 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    33781 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    33841 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    33901 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    33961 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    34021 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    34081 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    34141 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    34201 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    34261 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    34321 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    34381 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    34441 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    34501 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    34561 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    34621 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    34681 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    34741 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    34801 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    34861 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    34921 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    34981 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    35041 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    35101 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    35161 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    35221 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    35281 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    35341 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    35401 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    35461 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    35521 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    35581 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    35641 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    35701 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    35761 tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa
    35821 aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga
    35881 atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa
    35941 gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg
    36001 aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa
    36061 cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt
    36121 gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat
    36181 tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt
    36241 cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga
    36301 gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt
    36361 ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc
    36421 gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa
    36481 gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg
    36541 ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt
    36601 tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc
    36661 cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta
    36721 gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc
    36781 tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat
    36841 ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat
    36901 acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag
    36961 gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa
    37021 tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa
    37081 ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt
    37141 tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag
    37201 ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga
    37261 cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt
    37321 tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag
    37381 ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt
    37441 gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac
    37501 attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga
    37561 atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat
    37621 ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga tcacagaacg
    37681 agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag
    37741 cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga
    37801 tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg
    37861 ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga
    37921 agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca tcgctgtgat
    37981 gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg
    38041 gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa
    38101 ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac tttatgttcc
    38161 ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt
    38221 cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat
    38281 ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt
    38341 tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag
    38401 aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg
    38461 aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct
    38521 catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata
    38581 tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt
    38641 ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag
    38701 tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa
    38761 ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa
    38821 aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg
    38881 tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt
    38941 taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa
    39001 tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt
    39061 catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg
    39121 ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac
    39181 ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact
    39241 caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt aaattagacg
    39301 gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg
    39361 aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt
    39421 acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat
    39481 tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg
    39541 ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact
    39601 tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa
    39661 gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat
    39721 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
    39781 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
    39841 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
    39901 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
    39961 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
    40021 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
    40081 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
    40141 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
    40201 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
    40261 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
    40321 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
    40381 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
    40441 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
    40501 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
    40561 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
    40621 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
    40681 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
    40741 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc tgcagcagca
    40801 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggtgg cgcaagcttg
    40861 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
    40921 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
    40981 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
    41041 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
    41101 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
    41161 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
    41221 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
    41281 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
    41341 catcaataaa aaagcaaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
    41401 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
    41461 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
    41521 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
    41581 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
    41641 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
    41701 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcatttttgt cattgttaga
    41761 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
    41821 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
    41881 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
    41941 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
    42001 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
    42061 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
    42121 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
    42181 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
    42241 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
    42301 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
    42361 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
    42421 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
    42481 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
    42541 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
    42601 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
    42661 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
    42721 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
    42781 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
    42841 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
    42901 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
    42961 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
    43021 tctctgatat cgctgttgca acaaatgctg gccaaatcaa aactggttct ctatcacgta
    43081 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
    43141 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
    43201 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
    43261 ctctctactt gctgaaatat cagcaggtag agagttt
//
LOCUS       DASJRQ010000011        40840 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJRQ010000011 DASJRQ010000000
VERSION     DASJRQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.011"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..322)
                     /locus_tag="U4A80_000560"
     CDS             complement(<1..322)
                     /locus_tag="U4A80_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1974227.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQ"
     gene            complement(494..1195)
                     /locus_tag="U4A80_000561"
     CDS             complement(494..1195)
                     /locus_tag="U4A80_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1974228.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1211..1651)
                     /locus_tag="U4A80_000562"
     CDS             complement(1211..1651)
                     /locus_tag="U4A80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1974229.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1651..2226)
                     /locus_tag="U4A80_000563"
     CDS             complement(1651..2226)
                     /locus_tag="U4A80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1974230.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(3074..3553)
                     /locus_tag="U4A80_000564"
                     /pseudo
     CDS             complement(3074..3553)
                     /locus_tag="U4A80_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(3712..4413)
                     /locus_tag="U4A80_000565"
     CDS             complement(3712..4413)
                     /locus_tag="U4A80_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1974231.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(4521..5930)
                     /locus_tag="U4A80_000566"
     CDS             complement(4521..5930)
                     /locus_tag="U4A80_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1974232.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(5978..7843)
                     /locus_tag="U4A80_000567"
     CDS             complement(5978..7843)
                     /locus_tag="U4A80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1974233.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(7964..8816)
                     /locus_tag="U4A80_000568"
                     /pseudo
     CDS             complement(7964..8816)
                     /locus_tag="U4A80_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(9126..9839)
                     /locus_tag="U4A80_000569"
     CDS             complement(9126..9839)
                     /locus_tag="U4A80_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1974234.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            complement(9907..10233)
                     /locus_tag="U4A80_000570"
     CDS             complement(9907..10233)
                     /locus_tag="U4A80_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1974235.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            complement(10355..10603)
                     /locus_tag="U4A80_000571"
                     /pseudo
     CDS             complement(10355..10603)
                     /locus_tag="U4A80_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(10904..12157)
                     /locus_tag="U4A80_000572"
     CDS             complement(10904..12157)
                     /locus_tag="U4A80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1974236.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            12218..12958
                     /locus_tag="U4A80_000573"
     CDS             12218..12958
                     /locus_tag="U4A80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974237.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(13044..13802)
                     /locus_tag="U4A80_000574"
     CDS             complement(13044..13802)
                     /locus_tag="U4A80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1974238.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(13799..14827)
                     /locus_tag="U4A80_000575"
     CDS             complement(13799..14827)
                     /locus_tag="U4A80_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1974239.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            complement(14847..15455)
                     /locus_tag="U4A80_000576"
                     /pseudo
     CDS             complement(14847..15455)
                     /locus_tag="U4A80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15538..15732
                     /locus_tag="U4A80_000577"
     CDS             15538..15732
                     /locus_tag="U4A80_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974240.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            15722..16075
                     /gene="tnpB"
                     /locus_tag="U4A80_000578"
     CDS             15722..16075
                     /gene="tnpB"
                     /locus_tag="U4A80_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1974241.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            16177..17724
                     /locus_tag="U4A80_000579"
     CDS             16177..17724
                     /locus_tag="U4A80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1974242.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(17797..20370)
                     /locus_tag="U4A80_000580"
     CDS             complement(17797..20370)
                     /locus_tag="U4A80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974243.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     K"
     gene            complement(20592..21476)
                     /locus_tag="U4A80_000581"
     CDS             complement(20592..21476)
                     /locus_tag="U4A80_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1974244.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(21571..22008)
                     /locus_tag="U4A80_000582"
     CDS             complement(21571..22008)
                     /locus_tag="U4A80_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974245.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(22225..23136)
                     /locus_tag="U4A80_000583"
     CDS             complement(22225..23136)
                     /locus_tag="U4A80_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1974246.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(23513..24151)
                     /locus_tag="U4A80_000584"
     CDS             complement(23513..24151)
                     /locus_tag="U4A80_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974247.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(24155..24925)
                     /locus_tag="U4A80_000585"
     CDS             complement(24155..24925)
                     /locus_tag="U4A80_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1974248.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(25343..25759)
                     /locus_tag="U4A80_000586"
     CDS             complement(25343..25759)
                     /locus_tag="U4A80_000586"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974249.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(25810..26191)
                     /locus_tag="U4A80_000587"
                     /pseudo
     CDS             complement(25810..26191)
                     /locus_tag="U4A80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26317..26982
                     /locus_tag="U4A80_000588"
     CDS             26317..26982
                     /locus_tag="U4A80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974250.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(27379..29412)
                     /gene="brxL"
                     /locus_tag="U4A80_000589"
     CDS             complement(27379..29412)
                     /gene="brxL"
                     /locus_tag="U4A80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HEN1974251.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(29530..32052)
                     /gene="pglZ"
                     /locus_tag="U4A80_000590"
     CDS             complement(29530..32052)
                     /gene="pglZ"
                     /locus_tag="U4A80_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HEN1974252.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(32168..35800)
                     /gene="pglX"
                     /locus_tag="U4A80_000591"
     CDS             complement(32168..35800)
                     /gene="pglX"
                     /locus_tag="U4A80_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEN1974253.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(35864..39535)
                     /gene="brxC"
                     /locus_tag="U4A80_000592"
     CDS             complement(35864..39535)
                     /gene="brxC"
                     /locus_tag="U4A80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HEN1974254.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(39560..40135)
                     /locus_tag="U4A80_000593"
     CDS             complement(39560..40135)
                     /locus_tag="U4A80_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HEN1974255.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(40135..40734)
                     /locus_tag="U4A80_000594"
     CDS             complement(40135..40734)
                     /locus_tag="U4A80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HEN1974256.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT        12186 a         8207 c         6398 g        14049 t
ORIGIN      
        1 gttgaacaaa ataactttga gccgtccaat gggttcgaca gtttttacag gtatagcgct
       61 gcttttttaa gcgcataacc aaaggcatgt gattgtattg ttcaaagcgg acaatcgttt
      121 ccttcttccc atttttcact acaattactt tcccgtttcc atctaccaca gtagaaccac
      181 aatttctaca ggtatgggga gcaggtgaga aaacagcatc gacgatcaac gtctttttct
      241 tctgaaaggt ctcgtaagag acctctgtaa tcatcaaatc tttctctatt aatcgcagca
      301 tttttttgat agaatcattc atatagcata tcgtcctctc agttgtttat tttgtcgtga
      361 tttaatcata ctagagaacg atatgttttt taatacctaa aataaaaatg gggctgaaga
      421 atcaattctg attcatcagt cccataaatt atagagccgt aatttttctt tcccgtcgtt
      481 tggtttttta tttttagcct aattcttgac cttttgcatg ctggttatcc gtaaaatatt
      541 ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg atcacctttt
      601 ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt attttaggac
      661 atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttca taataggtat
      721 ataacgtctc atcgctttct agctgttcat agtcacgccc acgttttttg atacgttcta
      781 gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt tttggagcaa
      841 catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt acttcgatat
      901 ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca tagatggatc
      961 ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat cgcttgttca
     1021 aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag agcggcaata
     1081 cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt gcaagcatat
     1141 ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact gacatacaac
     1201 ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat
     1261 attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta atcatcaagt
     1321 tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata gttgccacac
     1381 ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg atagcaacaa
     1441 caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc gcccatccag
     1501 cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa cttgtgactg
     1561 ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc tcctctgaaa
     1621 aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata ataactgatt
     1681 tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg aaaagattac
     1741 ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta ataattgttg
     1801 caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcgg gcgtcagacc
     1861 gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac ctccagggaa
     1921 aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga tcgtttctgt
     1981 aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc cattcggata
     2041 gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc ccataatccc
     2101 ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt
     2161 ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa aaacagtaag
     2221 tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg tgttttttta
     2281 aaataaaaga ctagattttg tagtttttta gtggtatata ggatagacgc cactatatat
     2341 agtatttaga aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa taaataacgt
     2401 gttttcatcg atatgaaatg acttacttat agaagatact gggatttcgt cctatatgcc
     2461 aataacgtgt attattttaa acaacaaata cttgtttctt ttattaacga aaaaatcatt
     2521 ttgttctcgt gttatgtttc actgataaat gatgtttctc taaataaaaa agccgacgaa
     2581 ctaaacgatc aaaatttgat catttttgtt catcagcttc tcctaaaaat ctttttgtta
     2641 acgataaata gctggatttg taaaaatatc acttgaatcg tcactgctgg aagaaaattc
     2701 agtgataacc gcacctgttg cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt
     2761 gtactgatct ctagcatgaa gaataatttc tttttttact gtataataac tttctttcct
     2821 ttttggaata gtacaggaaa gtttttttgt ttcctttcct tcgacataag gtataccaga
     2881 ccctttcgac aacgaaaggt tctcattttt ccttcatcgt catttcaagg agggtgccga
     2941 tgctctggac agtttcgatc gctcctaaac caaaatctgt atattcgatt ctttctaact
     3001 ctgattcttt cataccattc ctgtctcaag gtacatctat tgtacttttt tcttgtaatc
     3061 cattctgcct ctcctaacta attgtcaaat catcccaaga tatgtcttct tcctcttttt
     3121 cttcttggat gatttctgca ttcggatcta ctggtttttt caataataaa atggttattc
     3181 ctgtgacaaa agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag
     3241 cgactgcaaa cattcccgat gccggaatac ggctatctgc gccccaaaac atgcttaatc
     3301 cgccacctac tgtcccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata
     3361 cgatagggaa acatccctct gtgatctgac agatccccat aggaaaagcg gtcttcaatg
     3421 tatcgatttc ttgacgattg agtatatttt tacgaatgat tttccctaag aaataagcga
     3481 tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga
     3541 ttccttctga aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc
     3601 cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg
     3661 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttttttta tttatgtttc
     3721 ataatcaata tggtaaggat gcagctccgt atggtagacc gcctccgaat attcaatcat
     3781 ttccccaatt tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt
     3841 cagaattcct tgttcttctt ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc
     3901 aatagtatat ccttttttcg tcagcaaacg atacaaagat tctttgccaa attcttctct
     3961 gctgaattgc atcatttctt gtggaagata atgaacaaaa taaatataag gctgattgtt
     4021 caaataatat aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc
     4081 ttccttcttt atatggacca attgcaaatc cagcgtttct ttccggactt catatcggga
     4141 gttctccaag atttgcgtaa agcttgccgc tttggataat cgattgtaag gacgattgct
     4201 tagaaccgtc gtcccacgac cacttttctt tgcgacatat tcatctgatg ccagcagttc
     4261 gattgccttt cggacagtga ttttgctgac ttgaaacagt tcttctaatt cactttctgt
     4321 cggcaacatc gaaccgactg gatattttcc tgagaaaata tcttctttaa ttttatctgc
     4381 gacttccaag tatagtacac gtgtcctttt cataacgtca ccctttgctt tactattact
     4441 aaaagaatag cacataaaaa ataaaaaaag aacacaaatc acatgtgaga agtgtgttct
     4501 ttttcattca gctgctttaa ttacaagttt acgccatttg aggcaattac ttctttatac
     4561 caatcaaaag attttttctt tgatcgattc agtgtcccat ttccttcatc gtctagatcc
     4621 acatagataa agccgtaacg tttactcatt tctcctgttg atgcactgac aagatcgata
     4681 catccccaag gcgtatagcc catcaaatct acaccgtctg ctactgcatt agccatcgct
     4741 tcgatatgac gacgtaaata atcgatccga taatcatcaa taatcgtgcc atcctcttcc
     4801 atatgatcaa tggctccaag accgttttca acaatgaata atggtttctg gtaacgattg
     4861 tataattcat tcaatgcgat tctcagccct tctggatcga tctgccagcc ccattcactt
     4921 gcctcaagaa acggattttt tactcctccc agcaagttcc cttctgaagt tgctgcttgt
     4981 ggatcagtct cattgattgc cgtagacata taataactaa atccgatgta gtccactgga
     5041 tattctttta gcaactggaa gtctttttcc gtttgctcta atttttccgg atcgactcca
     5101 tattttttat gggtacgcgc tgtgtatgca gggtattctc cacgtacttg gacatccgtg
     5161 cagaaaaaat tgaattcttg gttttggatc attgttgctg cctgattgac tggattggca
     5221 tcgatgctgt aagttgttgc atagataatc atgcagccga tttggatatc cgaacgcaat
     5281 tcgtggccga tttttacggc tttgctactt gcaacgaatt gattatgcca tgcttggaaa
     5341 atattttggt actctcctgc tccattgctt tttactaatc cttgacttaa agctggaaag
     5401 tgaaaggccg aattgatttc attgaaagtc atccaatatt tgactttact gctgtatctc
     5461 tctaatactg tctgagcaaa tcgttcatag aagtcgatca gttttcgatt tttccagcca
     5521 ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat cacaggttca
     5581 atatcatatt ttaagcactc atcaatcagc tgatcataaa attctaatcc cgcttcgtta
     5641 ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa acgataacat
     5701 ttgaatccca tttccgcaaa caaggcaaga tcttctttga aacgatcgta atgatcgatt
     5761 cctcgatgat tcgggtaaat atatttttct tgatcaatcg tccagtcaaa ttcttcactg
     5821 ccgataatcg caaagcgctg cttacctcca ggcatagcat ctgcaacgga tagtcctttt
     5881 ccgtctcgat cataagcccc ttctaattga tttggcagca gttgctccac cccataaaaa
     5941 tttttctgaa aaacgtgatt gcatcctgat ttcctcctta ttttacaatt gccagaacat
     6001 cttcgtttgc aataatctct ttttggtcta attcaagtat atcggcaaat gcatcagtat
     6061 tcgtaatgac aactggagtg attacgctat agccagctgc tctaattgct tcgatgtcaa
     6121 aacgaatcaa taaatctcct gctttgacga gatccccttg tttgactaag atctcaaagc
     6181 cattcccatt taattctact gtatccatcc cgatatgaat caaaatctcc gctccgtcag
     6241 tcgttgtgat ccctacagca tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag
     6301 gtgcaactag ttctcctact tctggatcaa tagcgatacc ttttcccatc acgccagaag
     6361 aaaaaacttg gtctggtact tcactcaatg gcagtacttt acccgttaaa ggacttttta
     6421 aggtatgccg tgctgaagta atcggttctc ctgcattcgc atgtttgttt tctaattgat
     6481 tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag
     6541 caattcctgt agctattact gctactgtca catttgactc tacaccatcc accgtattaa
     6601 tgaaagtcgg aatcgtcaat aagctgacca aactgctgga gaaagctttt actccggaaa
     6661 atccgataat agctccacca atacctccag aaatacaagc ggcaatgaat ggtttcttca
     6721 aaggtaatgt cacaccatac acagttggtt cagtgattcc aaataacgaa gtcactgtag
     6781 aagaaatccc taatgctcga agtttcgtat cttttgttcg caatgctaca gctaaagcag
     6841 ccccaccttg tgccaagatg gctggaagaa gcattggcat caatacatca aacccatatt
     6901 gttcgatatt caaaaacatg atcggtacga atccccaatg catgccgaac atcacgaata
     6961 cttgccataa acttcccatg attagacctg ctacgattgg actaaatcca taaatcgagt
     7021 tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg
     7081 ttaatggaac catcactact aaaacgacaa gcggaactaa aatcatttgc agaaattgcg
     7141 gaatcacttt ttttacgaat ggctctaatt tactttgaac ccatacggct agaatgattg
     7201 gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca aagaaactga
     7261 tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc atagcaattg
     7321 ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca agcatgacag
     7381 gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta ccgctatcag
     7441 ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc ccggcggctg
     7501 ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga cgattcaaaa
     7561 tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca tccacgatcg
     7621 attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg ccactttgta
     7681 cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa gattcatctt
     7741 ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc ttttgtcctc
     7801 ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt tccttctttc
     7861 ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc
     7921 tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat tggatgcttt
     7981 ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct gcgatcccca
     8041 tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa tgaaacgctt
     8101 ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca gatccttccc
     8161 ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga taattcaaag
     8221 attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata tctcttacca
     8281 ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat
     8341 gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt tgtttcacga
     8401 acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt gtttcaaata
     8461 ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa tgaagatgat
     8521 cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct tcagccagtt
     8581 cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc agctgctgga
     8641 attggttttt agcaaaagaa tctcttaaaa taaattcttt ttggaccgct tctggcgaaa
     8701 tctcgtcccc aatctttttt ttgaaaagct agtccttttc ccatacaaac gacttcttcc
     8761 ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat tttcattttg
     8821 cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc ttagctgaat
     8881 tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat cataccatcg
     8941 aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac acaagtctat
     9001 ctagaaaaca accaaatcag agatgcacct ctttgcgttc ctcttttttt cagcaaattg
     9061 gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac ttatgtccca
     9121 gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc tgtcgcatct
     9181 tttatcggtg taacactttc cagttctcct tgtacaacta gtcgcgtgac ggcgtccatg
     9241 tcgccacaag taattagtgt gacaatttct ttttcctcga cttcattcag taattgggta
     9301 tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt ttctaaatct
     9361 gtcaaataga tcttggcacc catctctaaa ttttccagcg gtgtaaacaa caattcaggg
     9421 ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg atctggagat
     9481 aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaat cggtaaattg
     9541 attgaaactg aagggactgc gatactagca atcactggta gatctgttcc tctcatctgc
     9601 gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa actgacatct
     9661 ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata tttatctcct
     9721 gtctctctga caagataatc cttgatctgt tcattgaaaa tcaaagctag tcctactaat
     9781 aacaagagga acaagaggat attgactagc catctttttg cttttccttt ttttttcatt
     9841 ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca gccgaatgac
     9901 acatttctaa aactctgtgt atttttttcg tacacctgcg cttttttcta caggatattt
     9961 caaattattt ttgatcaatt tttcttcaat gatctcatct agatccagct gaaggttgtc
    10021 tgccatcatg tatgaataga tcaatacatc cgccagttct tctttgattc gttctaattg
    10081 tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt cagatgcttc
    10141 caaagaaata gaaatagcca aatctttttc gttatgaaat tgtcgccaat tacgttcatc
    10201 tctaaactgg ttaacttttt ccatgctatt catttgcttg ccttcttcca atcatttctt
    10261 acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta aaaaaactcc
    10321 tgttttcttt tatttaaaat aaatgtcata gaaatcagta atttaggtag ccaccatgtg
    10381 tatgcagtaa tgtgacttct tcccctgagg ctttttgttt ttcgatacgt tcttctcccc
    10441 aaacagacat cgctactaga atctctctta atgattttcc ttcttcggtg agcgagtact
    10501 ctactttagg cgggacttgg ttatagactt ttcgatggac aatgccatct tgttctaact
    10561 ctcttaactg ctgggtcagc attttttgtg tgatcgtagg gattttcttg cttctccacg
    10621 cctaaaataa gcgatattgc tcttcccttt atctggtgta agcaaagcag ttagcatttt
    10681 gacgatcgtg ggttccgctt ttcacttctt atctacttag tatcatgaag tatactttcg
    10741 tactaatact aagtagcgga agtgtcaact actttgttta actacatagc aaaagaaaca
    10801 gaagggtgac aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct
    10861 atctccgtgt agactttcct caagcttatc ctttgacagt aagtcagtag gctgatttgc
    10921 tctttctaga tgccagatac atttctacaa aaaaatagtg attctgtttt gaaaattcga
    10981 tttttagttc tgcattttcg taaacatcca acattttttt cgcactgtat agacctaacc
    11041 cgccattttt tttagaggtt tttcctcttt tgaccatctc cgatagcgtc aaattcgtcg
    11101 gatttgcctt gttcgctata ctaaaacgta atccctcttt ttcttccagc agattgatcg
    11161 taatcggact ctgatcggct acacgatgat cgaatgcgtt agaaataaaa atagataaac
    11221 agcgaataaa aacaaccaga tcaatcggga ttgttttcac ttcgacagga ataactactt
    11281 ggatcgggat tttctccgat tctgcttttt ctagaaattt taaaaagaca cccctgacag
    11341 ctggggatac aatatttgtt agctgttgta tttgcggttt ttcaaaaata gctgcagaat
    11401 gatcttctaa gatttctaaa aattccaacg tttctttttt attttcctga agagtaattt
    11461 ttaatgaaaa cagtagactt ttataatcat gtttgaattc ctctagatga tggtaatatt
    11521 cttcatttat ttgatacagt tgaagcgatt cctgatgttg tctttctttt acttggaaac
    11581 tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat gactatcgac aaaagagata
    11641 gaagcagata attagatgag cggaagtgaa cacttcccaa taccaagtaa atcacgcccc
    11701 agcttaataa aatacaaata cctccaaaat agtgttcagt tggataagta aaaagcaagt
    11761 tgagaaatct cgtctgtttg tctatacttt ttactaatag atagaaaata aatgcgataa
    11821 gtagtgcaaa aaaacttgct gttccaagat gtctctttcc taaataatcc atcagatcaa
    11881 aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag attactgatc aaacagctga
    11941 taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa taataagcca actaggtaag
    12001 gaaagctcac tttagggata ggaaataaaa gtgtcaccac catcaaagct ataaaaccaa
    12061 agctgttttt tactagcagt tgtttcgaaa agaataaata gatacctaaa tctaaaatca
    12121 aagtaaaggt caaataaagt gtcactgttg cgaccatctt attcctccat tgttttgttt
    12181 caaaatgtac acatattttt ttaataggag cctgcatatg aaaaagtatt ttatcataga
    12241 agataaccag tttcacagcg aatggatcat caaagaattg gtcaatcatc atatcgagaa
    12301 tatcgtgcag ctcacttcta tctctcaaat caatcagttg atccagttgg gtactattga
    12361 taatcacgat gttttcttta tcgatatcga cttgaagcaa tcactcacag gtattgatgt
    12421 cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt gtttttttca ccgcttatac
    12481 gaattttgca gtagagatca tcaatcggaa aatccttccg attggatata tcagaaaaga
    12541 caggaattta tctgaacaaa ttcgtcaagt gctagaacag atcaattgga aagaacagca
    12601 aatcgtaaat gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc
    12661 tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac
    12721 aactacagaa gataaattag tgaatacttc tttaaaacaa ttgaagacag agttggaaaa
    12781 gtatgcttac tttttactat taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat
    12841 tgattctgct cattatgtgt tagaattttt caatggtgac tccttactcg ttggtcgaaa
    12901 gatctttgaa aagacaaagg aacgtttcca tgacttccaa aaaactgctt cgagttgatg
    12961 aataagagct gagacgtaag catagcagat ttcagggagt tggccttgaa tcggctccac
    13021 tccctgaagt cattgatttg attttacgct ttcccttctg taggagcatc tgttaacgac
    13081 catgtgaatg ttgaaacgta gttgtctacg gcataattgg atacaggaac aaataattcc
    13141 actgattgtc tcccttgttc cacattctct cctaatgaat aaatccatgt gccaatccct
    13201 tcatttgctc ctgcagttac tagatctact gcctgatctg ctgggatctc aattccttca
    13261 gcgccagtac cagaaatagt agatggcgtt ccactgctat tagttgcgct atttcgaatc
    13321 acggcgtttt tgaatttcac ctttgaacca gttagtttat tcgtatcaga tgttgtacca
    13381 ataaattctg ttcgtttgac tgatagtttc caaccggcat aaattccgct tttatccgtt
    13441 acttgtacga aattctgtac ttgtgttgct gtccctgtac tgtccgttcc gttttgtaat
    13501 gcagcatata ctgtttttgc tgtacctaac ttgattgttc caaaatcaag atctggagct
    13561 tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt cgttaggtgt aaacggcgta
    13621 tcgggatcat ttggatcaac aggaggaact ggaacaacgt cttcaggatt tgcggcatct
    13681 ggttttgtta cagaaatcgt tccttctgta gtggctgagc tcttattctc agcaaaaaca
    13741 ctttgtgtac ataataatag acttccagct agaacggtac ttaatgcaat tgtttttttc
    13801 atttcttttt ccttctttct tttaaaataa ataatccagc aaataagcca ataagaatga
    13861 ctccaacaag tatcagccat cgccagtcaa atgtttctgg ctgatttttt aaatcaatat
    13921 ccttttcatt caattggtcc gctgcatcat cagttatttc aaaatcggta tggagattcc
    13981 agcgtttttc tttccaagca cccttcacaa ccgcttgata atgtcccgga gctaattttt
    14041 ttccttctaa tgggacaagt aaatcaaaat ttgtattggg tgctacatct attttttctt
    14101 tttttattga aagataaggc tcttttgttt cttttttgta gatttcaacc gatgtagtca
    14161 agccgaacat ataagaagca tttggatttt gaagattcgc cttaatcaca tttcttccat
    14221 cgctttgatc cgcataagtt ttttttaatt tcaaatttgg ggaagactct ttttcgctat
    14281 ttcgaatcaa gattgctttc acttgagaaa actgattgac gacaccaact gtacttttgt
    14341 tagaaacgtt ctcttttttt tcttgaaacg tgattcctcc tgcgaccaca ccagccagct
    14401 ctttttctgg agctttcagt gaaagtgtct cagtgaccgc actttgagga gggacagtta
    14461 tttctcccac tgtttttgct aagtttttca tatcataact taatgtttca tcaaatttac
    14521 tttttgattc tccatattcg atcacaccgt ttgaattcgt cgtagcggag tggatcaatg
    14581 tctcaactgt tactgattta tctgttcggt taaagaccgt gtaggggatt tcctgtgttt
    14641 tcccaggctc catccttaca tttatatatg ttttgttctt atcaatctga ttagccggta
    14701 attcaaaatc taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc caaaggatga
    14761 atccaaggag aaggatcaga agtaaagagc agttgtttcg atgactttta ttccatattc
    14821 gattcatcaa tcattctcct tgatctttat tctgtcggcg catctgctaa tgtccatgtc
    14881 atctctgctg agtaattacc agctatctga ttttctttgg ttaagacaag tttaaacgcc
    14941 cgaccattat ttaaatattc tgaataatct gtttgcacat tcgttgaatt ttgtcctctg
    15001 acaatttcca gttcttcttc attcactaat tgatactcct cagctttctt gaaatatagg
    15061 acatcctgca tagaaaatcc tgcatcggtt gtgaaaggct gagataattt tatcgttaag
    15121 atccatggac tttttgtttc tcgctgatca taaatccata atttttcctg tatatccatt
    15181 tggccaaatg cttcttgcgt caacccagtt gctttgatct gaccacaatc gagtttggaa
    15241 ggggcaagaa atgagagacc accttctact acaatcttcg tagcaggtgc aaaaggcggg
    15301 gcatcatgat aaacaactgg cgtatcactt ggattgtgat tcccaatttc atcgttggta
    15361 cttggcttgt tctgcacacc cttagcagca gcttctcctg cttggtcgtt taatacaatt
    15421 tctaatacat ctcctgcaaa aagttgattt tctgggtaag cgccccatag aacagtacct
    15481 tactaacaaa tgcacctcaa gatatacttg agctatcaag aagtctggag gttttctatg
    15541 tataaattaa atgagattgt tcctgtccaa ttaggaaacg aaaagagtgc accagaaaaa
    15601 ggtaaacccg cggctgccaa gttagcgatg cgcatcaaaa gagagaatgg ttctgaagtt
    15661 ttgatctata acggtattaa caattatatt cttcaagcta ttttgaaaga actgttgtcc
    15721 aatgctttat gatttacttc aaaccaaaca tatttatatt gtgtgcggaa aaacagattt
    15781 gcgaaaagga attgatggtc ttgccagtct tattcaacaa gagtatcaac tcgagcttta
    15841 cgaagatgcc gtttttttat tttgcgggaa ccgtcaagac cgtttcaaac ttctctattg
    15901 ggacggtgat ggttttcttc tctgttataa gcggatagaa aacggcaagt taaaatggcc
    15961 tcggacgaaa gacgaagttc gaaccttaac gaaccaacag gttcgatggt tgttagaagg
    16021 attaagtatc gatcaacctc gagctatttt gccaggaaaa aaaggagttt tttaagctgg
    16081 ggggttaaca caaaaaatgt gttgaacccc tatttcttgt gcgtttcttg tggtattctg
    16141 taacaaaatc agttcgattc aaggtggtga aaaattttgg aaactacaga tacgttgctt
    16201 cagttgcttc aagaagccca taaaaccaat caagcacaac aacaaaccat tcagaatctt
    16261 acaactgaaa tccaactatt gaatgaaaag gtcaattatt taaccaacaa attatttgga
    16321 cgctctaaag agactctctt tgaggaaacg aatgggcaat tgaatctttt tagtgatgaa
    16381 gaaatttctg taagtgttcc agaagcagct gccaccatta ttccagtaaa aggacatcaa
    16441 cgagttgtag gaacgaaagc agacaaaatc aaacacttac ctataacaga gaaagaacat
    16501 ctccttcctt tggaagaaca attttgtgaa cattgcggtt cacagatgaa agatatcgga
    16561 cggacaaaag tacgagaaga aattcgtttc catcaggcta tgttggattg tctcacccat
    16621 tatcaacata cctattgttg taaaagctgc gaaaaagaag gactttcttc ttttaaaaaa
    16681 gcgatcgttc ctaaaccttt gatttctaat agcttaggtt ccaatagttt agtggcggaa
    16741 acgatccgga tgaaatttgg tcagaaagtt cctgcctatc ggcaagagaa ctattggaaa
    16801 caaacacatg ggttggatat ttcgagagat aatatcacca attggcatat taaagcagtg
    16861 caaaacgcgt tagacccttt aggagaacga ttaagagtct atcttaatca agaagagatt
    16921 ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg ccaaaacaga tacctattac
    16981 tggcagtttt gtacaggaaa agacagtcag catcccatcg tttattatca ccacgacgaa
    17041 agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt ttacagggta tttacactgt
    17101 gacggctata gcgggtataa tgccgtggaa agtgtacgac ttgtctattg tttcgcgcat
    17161 gttcggcgga aatttttcga agcaattcca aaaggaaaga aaaacacgga tattcccgca
    17221 gctcaagcag tcaaacaatt ggataaatgg tttgtcctag aaaagaaatg gaaagacttt
    17281 tctcctgaga agcgattaag ctgtcggcaa caagaactgc gtccattatt tattgctttt
    17341 tatgagtgga tggcaacgat agatcctgtt gcaaaatcca agttggatgc agccgttcaa
    17401 tatgcgtgta aactacgaag tggttttgaa cctatttttg aagatggtcg tttagaactc
    17461 acaaataatc gtgcggagcg aaatatcaaa gagctggtga taggacggaa aaattggttg
    17521 cattccacaa gtcttgaagg agcacgaact tcaggaatca ttctgagtgt atataaaaca
    17581 gcagaactta acggattgaa tcctgtaaag tatctagagt ttctatttga taagatacca
    17641 aatcttcccg tactttcagc agaaacttta gatcaactgt tgccttggaa caaggatgta
    17701 caacaacatt tttctcgtaa ctaaaaacgc caaacattcc cgattctatt ctataggtcg
    17761 ggttatttga cgtacaggta ctgttctatg gggcgcttac ttttctggga tagaaagaac
    17821 aaatttacct gtatcatcaa tagttgtttc ggacaaaaca gcttgatttt ttttcaatga
    17881 aaccaaagct cctgcttcgc ctgtacctgt gattgagcga gaagctgcat tgatcaatgt
    17941 ggtacttaat ttagcaggct caggaggaat cacatcgact gtcgttttgg cagagtccag
    18001 ttctgtcact tcttggccgc ctcttacttt tttagcagca actatttgca cttgatcccc
    18061 cgttgttagt ctttcttcgt tgggtacttg aacctcaaac aaaccgcctt gttcgccttc
    18121 tccccacgga ttatatcctg tcccttcatt ttcatctgtt tgtccttgtg tgagagcagt
    18181 aatttctaca ggtgatttcg tcgtatctgc ataagtaata cgtaaagtca cttctacttc
    18241 gcctgtccaa gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata
    18301 gattttttta tctgcattcg ttggtactct taaggattca atcatcggaa gctggttatt
    18361 ggcactgatt cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa
    18421 acctgcacta gggcttgata caaaattacc taagtcggtt ccattgatcg ataagtctgt
    18481 catatcagaa aaataatccg gatctttttc aaagttatcg aacgttactc ctgcctttcg
    18541 ccataaagca atttctgaat gagcgatctt caaagaagaa tcgccatttc cttgaaatac
    18601 ccagccacct atgccattag tctcgcttcc gatccgtttg ttttggaaat cgaaataggt
    18661 aggagattct aagtctacat tcaacacatc cccactgatg atgccagtct tatcttcccc
    18721 agttttcccg accatgacta aataagtttt ttcgccagcc ataaagttca aatcaatatc
    18781 tccagttgta tctacggtag ctccggaatc tgcgcgtata tcgatatttg atttttctcc
    18841 agtaaccgta aaatcagctg accctggact ttctagaaaa ccagaagcgt cataaaattg
    18901 gatcgcttgg tttccttgat tgagtctgtt cacataagat ttgccatccc ctgcatttct
    18961 taccttcaat gagcctccat tagttactgt tagtttttgc gtaccgttct caaaacgaac
    19021 tgcgctagaa cgaccacttt gcttatcaat atcgatgact gatccggctc cttctacgat
    19081 caaagaaagt cctttcccta caaaacgcat cccattgttt ccaggttggt cgccatctcc
    19141 agccattttg accgagattt gtccaccact tgatacatgg atttgactag aatctccttg
    19201 caggcgaaat ccgccagtag tcgctgtatt gattgtgatt ttcccatcat tcgacacagc
    19261 gactcttgat cctgagcctc tcatttggat aattccgcga ttgttgtcgt tggtaatatt
    19321 gatctttgta tttaccgaaa attcacttcc tgagccactg actttaaatg tgacattccc
    19381 gggtagcgta tcgacagcct ctccttcatc gctgacgcca tctgcccgat catagtcaag
    19441 agagaccttt gaaccactca agatatggag tcctggtgct gtttttccac tgcccgtctc
    19501 atcacctgaa tctagaaaac catagaattt tcctagatat ggtccataaa gctgactacc
    19561 attgattaca tttagctgat agcttctaca aaaataagct ttacccggag cggcattcat
    19621 cccttctttc actggttgga catccaatac accggaaaca ccatctaatg tgacagttgc
    19681 acgaggggcg taaatcaggc ctaatttaga accatcatat aagtcaagtg tcccggtaaa
    19741 ttttatttct cccgtaaaat gatatctgtt tccccaaaaa tcggacatat aagcaatgat
    19801 aatggcattt ccatcgatat ttggtatcaa cccacctgga tgagctgccg ttcctttaaa
    19861 attctgaaac tcgatcgact gaggtgtctc attgatatag atgtgatgtg tgccgaaatt
    19921 atgagcaagt ccgccaaaat cgataacgat atctcgcttg atctccaatt ccctgtctaa
    19981 agtcaaaggc tgagttaaac gaatcttagc tactgattcg tctgcgagaa gctgcgtcag
    20041 ttcttctgtt gtggcagctt cgctgtaccc atccgctgaa acaactctaa cctgtacctc
    20101 ttcactgcta tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc
    20161 ttgccttgta ttagaagtag gcgcctcact ttgggattct gtagtttcag aagtaattga
    20221 ttcttctgaa aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgcttgt
    20281 tttctctaca tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga
    20341 cgcaatcagc ttttttacat attgattcat tctccaccat cctctctgac gttttgacaa
    20401 ttttatttta acgttttaat ttatttgtaa ttgtactttc atatttaaaa tatgtatttt
    20461 tatggcaaat ttttctgtta ggtgaaaatt tcttttttgt acaaaaaaag gctgtgacaa
    20521 aagtcttgtc acagtccctt attccgaaca accgatgtcc tcctgacaac tcttcggtca
    20581 caacctccat tttaacaata aacctgtaaa tgtgagggta acactttgat tgtcacaggt
    20641 aatttatccc cttcatctcc atcaacattc gtcgtcaatt cttcctcatc agctaatgac
    20701 acaactaatt gctcacaagt aagataggct agatttttcg tagattcatc tactcctttt
    20761 aataggtctg gcaatgtttt aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa
    20821 tggagcttac catcatccac ttttgcattt ggaagtaatg tttcaaaacc gcctactgag
    20881 ttagttagtc ctatcagcaa gatactgctc tcaatttctt ccttctttcc atcaacttct
    20941 acgtgaaacg agtaggattg tgtggaagct aactgtttga ttcctgacat gaaataagct
    21001 agttttccaa atttcgtctt cttctccgca tctacatcat tgattgcttc agggatagag
    21061 ccaattgcga cgacattcat aaaatagtcg tcatttattt ttccaatatc caatggcttg
    21121 actgattcaa tcgaaaaatg attgatcgct tcctcaggtt ctagcggtat ccctaatgct
    21181 cgagccaagt cattcaccgt acccaatggg aaaaatccga aatttggccg atgttcttgt
    21241 tcagcgattc cactgattcc ttcgttgact gttccatctc cgcccatgac aaacacacta
    21301 tgataccctt ccgtagccgc ttcgcgagta aagttttttg catcccctgc tttttttgta
    21361 tgcaagacga ccacttcatc aaacaccgat tccaattttg ctatcgctaa ttgctcaaat
    21421 tcctttgctt gttcaccgcc tgaactgggg tttaccacta ataaaacttt tttcatcact
    21481 ttctttccct tctgattgat ttctttaata taacgatagc ataaagaaaa aaattaggct
    21541 gtgatgaagt actgttcatg aaacattcat ttattttttg acaagataaa atacccatac
    21601 tatgaaactg aggaaaatta aaacaatgat ataggaaata aaagaaaatg ataggcattg
    21661 tatacctaat aaagtaaaaa tcttaggtgg cagataaaaa cacaaaaata gcaagagaat
    21721 gtaatagcgt ttcttccact ttttgttatc agaccttata gcgatcagag gaataccaaa
    21781 tgcataactg aaaaactctt gatgtactgt taatatgtca cgaccttgaa taataaatgc
    21841 aacaatcccc gctataatta gtgtgcctaa taccaaaacg gaaaattgat gaatatattt
    21901 tatatacaaa tgatccattg tacttcctgc ctttttattt gtgagaaaaa caaatccgta
    21961 tacgtgaata acttctagaa ataaaagaaa tttgcaaaac cactccattt atctacttct
    22021 acgtgcattt tttaatatcg ttttctttat ttaagattat acagaaaaat atctagggtg
    22081 gctggattct aagtaaaaaa aatttttaaa ataaaaagaa ctaaatcaag taataattaa
    22141 actactgttg atttagttct aataaaaaga tttagatgaa cagttttatt gatttaaata
    22201 gttcatattt tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa
    22261 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
    22321 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
    22381 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
    22441 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
    22501 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
    22561 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
    22621 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
    22681 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
    22741 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
    22801 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
    22861 aaatgaatca cccaattgtt tctttgtttg atcatattgg gaatcgattt ctttggttga
    22921 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
    22981 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
    23041 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
    23101 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
    23161 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
    23221 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
    23281 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
    23341 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
    23401 ttgtttttct tcttccgctg ttgggatatt caaatcagac acttttatgt tcgattttcc
    23461 agacattttt ttgttgtttt tattagaaaa agtttgtttt ttcatttgtt ttctaaagca
    23521 tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgaccacg ataactaacg
    23581 taccttggga agcctgttgc gataagattt caacgacttg ctccttattt cgtttatcta
    23641 atgatccagt tggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac
    23701 aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt
    23761 ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat
    23821 tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa
    23881 aatcttgaaa aagaaaagag agggcatttc tttttatcaa gcgcttctct cttccagtta
    23941 aatgttttgt ttcttgatta aataaataaa attgccctga actgaattga tcattcaatc
    24001 ctaatatatt caataaagta cttttccctg ctccgctctc tccaactaca gtcacaaaat
    24061 ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt
    24121 tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc agcaataact
    24181 gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa
    24241 agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac
    24301 ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg
    24361 aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata
    24421 acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt
    24481 aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg
    24541 ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg
    24601 acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt
    24661 cccaatacgt actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg
    24721 ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt
    24781 ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg
    24841 tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga
    24901 tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta
    24961 tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac
    25021 ttccgttttt attcaaagtt tcatataatt cctgatcggc tgctgatgca aaacgattgg
    25081 aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct
    25141 caccagcgat ttcttttggt gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga
    25201 tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa
    25261 aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata
    25321 aaagagaaat caaagagata ccctatattc cgctgaacaa gaaaacgtac atctggaaca
    25381 aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta
    25441 tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa
    25501 agccattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca
    25561 aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtac
    25621 ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat
    25681 cagtgtgaaa tgcttgattg aatcgttcca caaatagtaa aatgaaccgt tcataggctt
    25741 ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt
    25801 caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga
    25861 catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg
    25921 tatctacttc attggattca tttactgttt ttttcatata agaaagctca gtttctgatg
    25981 agacattttc aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg
    26041 gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta
    26101 cctgtttatt tataataacc aaggcaaaac cactcatgat accggtcact atgattgtga
    26161 gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga
    26221 taaaatcata ccgtttttca tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa
    26281 taaatctata tactgtagaa tcgaaaggag aagaagatga acatcggaga aaaaatacga
    26341 acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc
    26401 gataaaacaa tctctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca
    26461 cttttacatc agactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa
    26521 tcaatagaac caacattgcc ggatgaacct cttgttatca agaaatctta ttcatcaaaa
    26581 caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat
    26641 ctttttctta tcaattatca atataaaaat gaaacggttg atcgattcaa tccttttttg
    26701 aaaccacaaa tcggctatac cacactccct gtaggatacg tggaaaattc catcgaacat
    26761 tgggtatgta atgatatctt tgttcatggt acatggctgt cttttcacgc tggagaggta
    26821 gaaaaaggta agaatcatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc
    26881 ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcatcca agactatgac
    26941 gatgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga
    27001 gatagaaaaa atcaatgcac atgatcttat caaaaaaaaa caggggtaca aattaattga
    27061 aaaagctttg agatgaaagt tcctaaataa aaaagttcag aatgataggt aaaaaaatga
    27121 ctaactattc tagtattagg ctatttgtat tattcttctg ttatttttat atcaaaaaat
    27181 ccagaaataa ctaaatatag gtatttctgg cttatagtat aatatttacg ccacagcccc
    27241 caatgattct ttttggaatc atgatcaagg acggacatac acaaaaatat tttgcccatt
    27301 cgatccgaat agattaacga ctaatctagt aagtaatata gattggtacg tagtcaaacc
    27361 agcaaatgga aaagttgtct attccacccc aagggcttta aagacagcat cttccggcgt
    27421 ttgataaaaa atcaaattaa aggtacccat taattctggg ggtacagttc caatatcagc
    27481 agcgcttatc ataggtacaa gtactttttt cgctccggca tcttttgcta cttgtaaaca
    27541 gttagctaaa ttatctacct taatcataga accaccaatt gtaatatccc ctaaaataac
    27601 taaacttggt aatacaggtt tatttaagga aactgagcat aaagctatta tagttgataa
    27661 cgataatgtt tttgtcatac caatgccatt catatcttga acatgaacga caaaatcttt
    27721 tgtcgtcaaa ctaatagact gactaacact tccataatta gctcttaaat atcggtatgc
    27781 cgaatccatc gcttctcttg ctccagcatc tgaattgatc ccagtacgtt caaactttcc
    27841 atttcccgaa gtcatttgcg tttccaattt ataggtacct aattgcccag ttttgccata
    27901 agataccgtg tacaaatgcc ctggcttccc taacccttct ggaatcaaag ttcctccacc
    27961 ttgttcagga acagaaacat actcttctcg ctcttcttct aaatcaatgt atgaaaaatt
    28021 gacatcataa aattccattc caccaatttt ctttaattgt tcttttacac gacgacgcat
    28081 ttcaagagca aatcttagta tttcttcgac atcacttttt tcaaatctac catcaggata
    28141 caacagtttc atcatcccag atacaatccg acgaactgca atcgtatccc gctgatttaa
    28201 ttgagaacca agtttgaaat atttatcaaa actatcgcca aaactaactt ttcgaagctc
    28261 acgcatgact tcagaaaaat aatctgtaat gaagccatag tcatttgtaa aaaaatctgg
    28321 tcgatattta ggaatttccc aacctggtaa ataacaatgc atccgatcca aaaaggctga
    28381 atccgtcccc ataacctccg gaaatggctc aaataaattc gaagttttca atattacatc
    28441 aacgctttga ttaatatttc ctacaaaagc cattgatgct gaagcattct tttcttcttt
    28501 gcctctagca aaagatcctg aagccatgta atctttcatg atttgaacgc catcattatc
    28561 cttaaattta atcccagcaa cttcgtcaaa agcaacacag tcccacatac ctacaagtcc
    28621 cattgtacga ctagccatat tatagaaaag gtttgctact gttgtttgtc cacctgaaac
    28681 aaggattgag tttggtgaaa tttctttata tacatgcgat ttacctgtac cacgtggtcc
    28741 tagttcacag agattaaaat tattttcaat taatggaata agtcttgcta aatgaagcca
    28801 ttttaccctt ttattaaatt gtgtaggttc cataccaatg gaccgtagaa gtaagtcaat
    28861 ccattcattt gtagtaaact ccttgcgagc catcttgtaa gcctcaatgt ctaaaccagg
    28921 catttggatt ggatcaacct tttttgcaat aaaagggcta ttccaacgat cattctcatc
    28981 ataccaatat tccatcgaaa caatacacca gatcccccca cctaataatc gatcaaattt
    29041 tttcggatac tcttcactaa tttgaacatt ttttaatccc aggttagaga attctgcttc
    29101 ataacaatct ttcttttcat ttaaatgtac agttacttta tcaataactg tataaccacc
    29161 caattcacga atttttgctt ttactttttc tgcttcatca ggacgcacat agtttcgagc
    29221 aagaatattt ttaacatttt ccacaccttt ttcaaccaat gtttcatcca atgacgaagc
    29281 atacatacca agtaaatatt ccaatacata aacaggtaca ttcgcacctt ctttaatttt
    29341 ctttgttaaa tcttttctga caatctttcc gggaaagaat tcagataact ttaaaatttc
    29401 ctgttgttcc actagaattc ctccatgtgt tcagtcatta taatagcaat taaagtatat
    29461 catacttaac cagtaggctt gataatacaa aagaaaaagt atccacaaaa aattgtggat
    29521 acctcgtttt tatatatcaa aatcaaatga ttctgcaaaa ggattatcta taatgaattt
    29581 tattcgctcg gtcaaatcat ttttttgatt ttttatcact aaataataag tatcttcaac
    29641 tgtatatcga cgatttacaa attcaaaaac aaatttagta aaccgctgat ttgcatcttc
    29701 tgtttttgaa tccgcaacat agatttcttc atttgagata cgttcaccat attcatcttc
    29761 aaaataaagc cagaaagatg cggggtcata aacatcacta attgcatttt tttgatagaa
    29821 ttctaacgtg gttgataatc ctgtaattct gcggttgcta gtcattaatt caattgctac
    29881 tggttccttt gcattgctac ctttggctac cgtaatttct agaacaggga ctagcatttc
    29941 ttgaatagat gaccctccat gaacgtagct ttgtcccccg ccagctttct taaaaatact
    30001 tgaagtaatc gggacacaaa tgtttctagt gtcatcattt cctaaagaat ctcccaaaga
    30061 gtaatttttc gtaccaatta tttcgtattc ttcttcacta agaataaaac gacgttctac
    30121 acgatctttt tcatttctag atggattttc aatcttatct acttcttcaa cgtggcttct
    30181 tttataaata aatccatgat cagccgtaac tataaaacga tagatactgc catcattatg
    30241 caagcgatta ataagtcggg aaatttcgct tatcgctaaa tcacaggcct caaaaactcg
    30301 attttcattc gagtgatcac ctgttctatc cacttgatcg tgataaatat aaatgactga
    30361 tttgccatta aatacttctc gtacctcagc cttattcatc ttaaccacat catcataatt
    30421 taaagctaca gcatttgcat ctcgttgctg gagaatcgca tttcgattgg ataaaccatt
    30481 cgtttttttc ccatcaatca gtacttctgt tccttcattg taagttagtt gattgtttgg
    30541 taataaggcg gctttaccga attcagtaac tgaaggtaat acagaaagaa agctagccat
    30601 gtttgttgaa tacttctttt ctctaactaa cttacgttct aaattcatcc ctaactcata
    30661 tcgaaaagca tctgaaataa taactacagt tttaacagag cgtttcatta cagcttgatt
    30721 ataaaatttt cgaatggatg gacgattctt aaattcaaaa gtttgattcc aaatttgtcc
    30781 tgattgatca agaaacttcg aataatttgc ttcaactaat cgatacaatt ggccatattt
    30841 ttgcttttct tctatatgat cactattcca aataaatttc cgataatgcg tatcaaccca
    30901 atacatttcc ttctcatatt gagttagcaa attatccatg ttcatcggca agcacaatct
    30961 gtttttatcc acaagataat aagcatgttt caataattga tactcataaa tataatccga
    31021 tgcgaaatgc attttttcgc gctgctttaa actatcaaaa attgtttgtc ctgcaatttt
    31081 cgttgtagtg tcagaagcct ttaatcgttc tgcataaaac attaatattt ttgcatgaat
    31141 ctcagaaaat acgtctgctt caattagatc atcaatttct atactgtcta ataatccatc
    31201 accatcaata gcctgatata cttttttaga tagctgttca aagctttcca tgtaacgtgt
    31261 atcattcata aactgatcca tgaaagtaat aatgtttgct ttcaaatgta gtatttcaaa
    31321 tcctttgaac ttcttcggca tctcttgatc aatttgataa taaaaatagt tcaaataaag
    31381 acacatcgtc agcttttcca atgttggctg gctatccgta aagccaaatt tcatttgaat
    31441 aaattcccaa aatttagctg ttaactgata cttatcaagt tcccttaaag gattctccct
    31501 actttctgta aagcttttta gaactacacg cagtaaatca tttaatcgaa tacttttcga
    31561 tttcaacaaa acagccatta tcattaattc aatcgtttct gatttattaa ttgtcatatg
    31621 cgtactaaat tcaccaaaac ttctcatgcg atcctttgca ttaaagaaat tacgatactg
    31681 tttaactgtc tgatgaaatc taagtggaat agccaattca ttcatgataa tcgctaagcg
    31741 gtctgcacta aattcctctg aataattcaa tacactcaac aaatgattat cttcatcttt
    31801 atcatcccaa cgaggaaatg gcgcatagat taaatagtta gtttcgggat cttcatattc
    31861 caataaacgc ttagttttaa attgtgcgtc cttttctaat cgatagattt ttgcattttg
    31921 aatgaccaat gagtcaattt cttcaagaaa atcctgattt gaatcaaacc aaaaaatgat
    31981 tttcctacat ccataattag gaaaacgttg atttaatttt tcttcaattt gttgcgtatt
    32041 tatatctgtc atcaatctca cctaatcgtt ctctttctat accccttata ttgacataat
    32101 ttggataatt tcgcacgcaa tttaagaata aaaaagaaag ttgttagaaa acaactttct
    32161 tagataacta tttttttggt tctttgaccc cttttgcaaa aatttggaat ttctcgccgg
    32221 ttttaggatc acgctgcaac ttatcataat tgacaattac accatcatct aagtctaatt
    32281 caattcgttt taatgctaag tgacccaact tctcgtcata ctcacgaact tcttttaatt
    32341 gcttctgaat ctttacaatt tcttttgtca ttgcatttct ttcttttggg ttatcacttg
    32401 ttgaaatttg ttgctgacgc aattcgattc gattgtcata cgctttttgt agttcgtgta
    32461 ggtattcagt acgtactttt cctaattgat ctggtgtata gcgatgcaag tacattaacg
    32521 ctttaaagcc attttgtttt cctgagtcca attgccaata aattggtcga ttggagtatg
    32581 tttttaaatg gtctttgtaa aaatcttgta cgaagtaacg acgaataatt tctcgacttg
    32641 tccctttgcc acctaaagtg tcagcgataa actggagatt ttcttcaagg gtttctttac
    32701 caaaagtaat ctcaacgaaa tcaatgaaac gattgacaat gtcaatttgg ctatccttga
    32761 aatactcatc atcggtgatg agtaaaacat tatcagtatc aggtttaaat ttttgatagc
    32821 gggaaggatc ccattcgccg ccagcgaaga tgagaccatc ttcgtctagg gagtaacgac
    32881 cgaagataca acctattgaa taagaaataa aatccttaat ttccctcgcc ttatcaggta
    32941 accttgcaga gatatattca tcttgaacct cttttgttaa ttctttttca agagaataat
    33001 gattaatgaa tatgtcgttc agttcctctt cattcgattt tagagtatta aattgattaa
    33061 ccaactgcct acttagacag ttaaatgcct ctgagagatc ttttttaaac atataactta
    33121 acaaaatgtt tcttctaaac tcgtatgacg tttcaagagc attatagtct tttctagcta
    33181 tatcgatatt ttcatccact ttttctctta ttttttttaa ttttctctca gatgtaactt
    33241 taagaaaagg tagttttccc attggtcctt catggtaatc caaggtggga gaaataaaag
    33301 ttaaaaagtg attaactaat ttagagttta catagccaaa tatttcttca aagtcagtta
    33361 tatcctttga gaacagaaca gatcctttag tttcaaaaat tgcctgattc tgcaatctaa
    33421 aagatacttg tccagatgaa agtgtcgacc aagtaattcc ctttttaaaa tagtaattaa
    33481 gattttgtgg tctagatttt aacttcccat tttcattaac ataatttttt atttcatctc
    33541 catcattctc ccagttaacc acgtattcat tgtttccata ccattttcta aaacgaccac
    33601 ctttagttgt aggaaaccat ttttttctag actttttagc gtcctctaat gttttagcat
    33661 caaactttat tttgttaaat tctacttcaa accaataccg caaaaattta ttgttatttc
    33721 cggttgccaa cccttgtttt gcatctgcaa catcgttagt taattttcct tgagcaaaat
    33781 cagtgattac gttatcgctt gcccagtaag caatcggcat tcctgggatt tttttgaagt
    33841 tgtcttgatt agtactataa aaataatcgc attcaccatg ctgatgaatt gcttctaacg
    33901 ttttaatttt ttgaacttcc attccacctt taaaatctga taattttata tagcttccat
    33961 cttgttcatt tgaatttttt atcgtaaacg tacaaattgg aacggttgct tcttcaaaag
    34021 ctgaatactc catttgaatc aaactagaaa tgtttctttc attaattaaa aatgagcgta
    34081 acttttcata gctagaaatg aacatccaaa caaatggcgt cataaaggaa gcatatccat
    34141 tatcaatcag catttggcta ttcaatttaa taaacacaga aaatagatct tttttgacat
    34201 cagaatagtt atcatttaca tattttttta atttcttatc cattttatta agataaggcg
    34261 gattagtgac taccgctaca tatttatcaa ctagcatttc agccacgtca agaatgtgca
    34321 agtatttttc ttgtatttcc ttcatcccat aaagaatatc catattcgta aattcgttga
    34381 attttgatat atactctctt acttttccta agtattcttt agttattgcc tgattatttt
    34441 tatcgactaa cttaatgatt gatccaagtt ctttcccatt ttcaaattga tcaaaaatta
    34501 actctttgta ttcattccag cgttcactat acttatcatt gttcaattca ctaatacgct
    34561 cttgatactc gtctgtcact ttaggagaat caataaactc tcttaaattc ggcgatagac
    34621 tttttttcaa aaaaagacga tcgtattgac gccctttcat cataattgca aaataagaca
    34681 attgatacgc tcttttatcg atttcaagac catataaatt gtattcaacg attgcacgtg
    34741 cagcatcacg ctcagaatat cctaacgtaa gataaatttc catcaataca tcgaaggcgt
    34801 aaactaaaat atgcccagac cccattgcgt tatcgataat ttttagttct tctggtttta
    34861 aattacttac agatgttgct tgttcaaaag catatttatc tggaattaag tatttcaact
    34921 tatttcttaa ttcactattg ggataatgat ctaaccaata ctttcctaaa ctattgtcaa
    34981 ccatataacg aacgacccaa tctgtggtaa atagttgagt tgctgccgga atatcttgtt
    35041 ttttgacaat ccctttataa atattcacca cttgattatg tggctcacta ttatagtact
    35101 gatacaacca cccaataatc tctacctgtc caccagcttg aacatagaaa tcatcctctg
    35161 gaatacttaa aagatcagcg attacaccat cttttttaat ataagaagct gtaaacaaaa
    35221 gttctgtata atcttctact tcttcaaata gctcaggtaa gtaatcattc aattgattac
    35281 agatttttat aaaaatcaat tgatacagct tgtccattgc ttctgctgaa ttatctgttt
    35341 tccaagttct gacttgttgc tgttcattac tagataattc ttcaaagata tcggcatcat
    35401 aaatatctgt aataatatct ggctcaattt tcccacttat ttcactagat aatacacgat
    35461 acgtgtcagg taaataatca tttacctcca taaaccgaat ggcaatcaat cggttaaacc
    35521 acgtataagc aacctcttcg attaactctt gaaaagcttc tgaataagaa gtgacagaag
    35581 cacgttgttt tagttctgca atcaatttgt ttctttgcgc tatttctttt ccttgaattc
    35641 ttattcctga tttttcgatt tcttgtaaat tgctatcaat tttcttcaca tcttcaattt
    35701 ttgattcatc aattcctaaa cgttttgctt tagtcattac ttcatcaatt aattttctac
    35761 gggcattgac cgcgaaattc tttatcgctt tcttatccat ttttctttcc cttctatatg
    35821 taacctttga aaagacaggc acctttttat gcctgtcttt tcactagaat tgaatcgtga
    35881 tttctgtatt ttcttgaagt tgttccttca gtttttgatt aatttcagct gttaactcat
    35941 caatatctgc tgctgactta atagttttaa catgaacgtt aagtgttctc attggtactg
    36001 ttttctgttg aatagtaggt tttactatcg ctatttcttc agcttttgct gcagagtcta
    36061 tttcatcttt ttcagtttca cgaatcaagt ttgctgcttc aatttgttta cgtgcttgtt
    36121 cttcttgacg tctgcggttt tcttcattat cgaaacgttg attaaagatc aaatactgtt
    36181 ctgctacgtt taatgataag aaaacaagat catctatagt attcgcttgt tcaatttttg
    36241 ctttcagttc ttgaaattct tggtcaacta ttggtacaaa ttgatcctta aaatcttttc
    36301 cttctaataa tttaaatgtt gcttgttttt cttccgttaa tttatttaat atttcctgac
    36361 tttccaactc taacagttga ttatattgtt caataaattc ttgaatcaac tcatttagtt
    36421 tcatcatatc tgaataagga tcagcctttt tcaaaatggt tttcatctca tgaatcaatt
    36481 gatcaatttc ttggctagaa gtttgacgcc gacttttttc gattaatcta attttatctt
    36541 gtgcattctc ccagattttt ttctgagtac tattttgctt gtggaaattt ttaattttat
    36601 catatgtttc agcccagtcg aataattcat cgcgatattt tttgattgta taaaaaaagt
    36661 cttcaacatc tgacgtttga acaattactt caagatattt ttttatttgc tcatattcat
    36721 aagcactagg gtaggtataa gtgttttgta aatcaaaata agagcgtaaa ttgtctaatt
    36781 gaccttttaa atatttcttg aaattttcca ttgtatgttc attgttattt tcatcaacaa
    36841 tattacgact ttgaaacagt tctctaccga tttcgtcaac caattttttt gcttttggcg
    36901 aaatcaccgc gcttggttca atttttatct tttcactttc tcgtcgtttt tgaataatca
    36961 ttacaatact atttggatca tccatccgtg aaatttgtac attgttataa agtaaatcaa
    37021 ttttaccaga catgaaaagt ttagcgatgc accattccac atcaagttct gtgaatccat
    37081 acggttgttt tgtttcaaaa cgatcacgaa ttgttttcat cgtaatcgtt aggtgatttc
    37141 tcttttgatc attgataaaa ttgaaaattt cctgaactgc ttgctcattt gatggatctt
    37201 caataatttc atttttgctt tctttcacta aatataagat atcttctttt tctttaggcg
    37261 catcaatttg gtttaattta tggtatactt cttcagccat tttctgaagt gctgattgga
    37321 attttgcttt aaagtcggag cctgtcagtt gaatttctgc accgttatag aaaaattttg
    37381 cagatttcaa cagttcattt aattgatcgt tgatacttgc acttagttcc tgtaattcac
    37441 cagatttttc cgaacgaatc gcgtcaaagt tctttactac actcgaataa gtcgttgtta
    37501 aataaagatt cattctagca tacattgcta tatcatcttt aaattcttta tctgatggca
    37561 attctacgaa taataagtta cgggaactct taagcgcaag ttctgcctct tgacctgcta
    37621 aatcagagta tggtgaaata aaattaactg tcatttcagc atttccagct tgcttgtatg
    37681 gacgttgatc taacacttgt gcataagtaa acgtataccg gtttttaaat ttatttccac
    37741 ctgacatgcc taatttttca taggcaaaac tatttcggtc tagaacgccg tcaaaaattc
    37801 gtttagatag ttctacgatc aaatcactat ctgtaatatt ttgattttta atctcacgag
    37861 taatttcctg ttcttcatca gtttggaaaa tataactatc taagttcttc cgcacaaagt
    37921 tttccttaat taaaaggtcc aatgcttctt ctactcgttt tttagtttcc aggcggtctt
    37981 cgttgatatt tcttatcatc aagctcgtaa tattttcaac atttggtttg atggcttgat
    38041 caacatattt aatcatgaat aaaattcgta agacatttac gttaaagtta tctatatcag
    38101 ttggtctttg aatatgagaa ttatttgtag cttgtgtaat cactcggcta attgctgtat
    38161 caatccaacg ttctaacgag taataaaact gatctaaaga aaccaaactg gttgtatcat
    38221 tttccttatt tcgttcagcg gcttctttaa acattgctag taatgaacgt tcaccagacg
    38281 ataagtttga tccttgatag ctgtgattac gaatctcggt tagaatttgc cccaataaat
    38341 tgaattgata gggaataaat ggataaactt ctgcgtaatt ttcagcatct tggaagagct
    38401 ttaattcagg actatcaaca aagagtaatt tatttttcaa ttctgtttga acagtttcaa
    38461 attgtaaggc taaagaatca ttggcaaact cttttttctt caaaatacgt tctttaatta
    38521 cttcatcggc atttgctgaa gataaactca cagttgtggc aaaacgatcc ttgatcttag
    38581 aaaaatcatt atcagaattg ttatcaaagt ttgttacgtg atcaatcgct tcctgacttg
    38641 tcactaatac ccaagcctta ccttcacaaa ttcgacccaa atcttccaca acggtttgta
    38701 actgcaaagt caactcacga ttaccgccga tatattgtcc gacctcatcg actagaaagg
    38761 caatatgatg gtctgcttct ttggaatcaa gatagctttt aactagattt gcaaagtcat
    38821 caatgcttat taaataattt cctgatgctt tttctaaaaa attatcaata gcggcttcac
    38881 tcataaaatc gattttaagt gccgcttctt tgatatcatc ttggatccaa tcaaactcat
    38941 ggcgctcttc ttgccatttt gccccattaa tctcttcaaa agcagctttg aatgcatcaa
    39001 acttttcttc tttaattaac cgacgttcaa aatctgctag atgtggaatc gtgcttaatc
    39061 cttgcatttc gttaaatacc tttaaaaaaa cattcacgat tccgtttttg tcttttttac
    39121 cacctgcatc actttttgaa tcaatattaa acagaataac atctgtattt atttgtgagc
    39181 accgttccat cgttgccaaa gtcagtggat ctttgatttt cccatcttct ttgaagtaat
    39241 caatggcttt cttgtcattt atttctcgat ttgcgagcaa gtaagaaata atttttaaga
    39301 aatgagattt acctgaacca aagaacccct gaatccaaac acccatttta tctgttggtg
    39361 tattgatact ccgttggtag gcatcaaaaa ttgttttaaa gtgtttttgt agttctttcg
    39421 taaccacgta ttcttctaat tcttgttgaa cattttcatc ctttgcttga ccgaccttaa
    39481 taactccttg aatatttcgg tcaattggtt ttgcaaaaat atctttaatt agcatttaat
    39541 ctcctacctt ccatcccaac taaattctaa atgctcgata ataattatca tctttattgg
    39601 tattaagaat tgtcaaactt aattcatcat attcccctgg atacatcatc acaactggta
    39661 cgcgatcaaa tacctgatgc attttattta aaatagagtg cgctctaatt aatggatata
    39721 attcgccaat tcctgtcagg aacacaactg atttttctgg tgtatgggca tccacatatt
    39781 caaaaaatac attttcttcc tcaggtaaat ccattttcaa taaacgacca atgctttcca
    39841 ctaatttttg taaaccacgt cgttcttcca tcttaatcgc tttttcagtg aaaccgcgtt
    39901 tttctagata ctctaacata atcacataga gattataaac tttcacttca taattatcac
    39961 caacagatgt tccagatata tcattaatat aatctcttac aattaactcg tctttcggat
    40021 cgtagtcaaa gatataatag cctacttcat tacctaaacc acgattcaat aaaaaatctt
    40081 ctttttgaat tacttcattc aacttttcca aatgctcttg aatcgataat gacattataa
    40141 aattcctccc agtgctagta caatatcttt acgattcttg tcaactaacc attgaatcaa
    40201 tcgtttatct aataatatcg gagtcattcg tccatattct ttttcccctc tgactaatcc
    40261 tgtttcaatt agattcgttt gataagcggc aataagccgt ttaatcgtat aatctttcca
    40321 ttttgaaata tcttctgaac ttctccgctt ctctgtaaaa aaagaacggt aatcaactgg
    40381 tgttaattgc aaatttcctt tacgtagatt ttcagcatat acctcagtca taaattcatt
    40441 aaaaagatca tcaatcataa ggatggaaat caaattcact aacttttgat tatctatatt
    40501 taactgattg aaaagttgca atccagcttc atccagttga ctaactctcc ttttcacgtt
    40561 ctgatagatt tctttttgac ggttactgct cactgcaaaa aaaatatttt catgttcatt
    40621 tttctcttta atttctatat ctgtatatcc ttctagtcgt aattgaatat ataacttgaa
    40681 ctctgaaaac caaaagcgtt tagaaactag accagcagaa tattttttat tcaatagatt
    40741 gcctcctaat catctataat tcaataagta aacaagtgtt ttttgctcat cgttagcatt
    40801 cattttttag tatctactat acaatacata tttattatag
//
LOCUS       DASJRQ010000012        38053 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJRQ010000012 DASJRQ010000000
VERSION     DASJRQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.012"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            66..767
                     /locus_tag="U4A80_000595"
     CDS             66..767
                     /locus_tag="U4A80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1974257.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="U4A80_000596"
     CDS             764..1885
                     /locus_tag="U4A80_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1974258.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="U4A80_000597"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="U4A80_000597"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1974259.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="U4A80_000598"
     CDS             3264..4172
                     /locus_tag="U4A80_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1974260.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="U4A80_000599"
     CDS             4207..4479
                     /locus_tag="U4A80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1974261.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="U4A80_000600"
     CDS             4490..5536
                     /locus_tag="U4A80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1974262.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="U4A80_000601"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="U4A80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1974263.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="U4A80_000602"
     CDS             complement(8547..9233)
                     /locus_tag="U4A80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974264.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="U4A80_000603"
     CDS             complement(9211..9705)
                     /locus_tag="U4A80_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1974265.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="U4A80_000604"
     CDS             complement(9725..10945)
                     /locus_tag="U4A80_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974266.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="U4A80_000605"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="U4A80_000605"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1974267.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..14034
                     /locus_tag="U4A80_000606"
     CDS             13123..14034
                     /locus_tag="U4A80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974268.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15030..15281
                     /locus_tag="U4A80_000607"
     CDS             15030..15281
                     /locus_tag="U4A80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974269.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15364..15636
                     /locus_tag="U4A80_000608"
     CDS             15364..15636
                     /locus_tag="U4A80_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974270.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            15726..15965
                     /locus_tag="U4A80_000609"
     CDS             15726..15965
                     /locus_tag="U4A80_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974271.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16112..20395
                     /locus_tag="U4A80_000610"
     CDS             16112..20395
                     /locus_tag="U4A80_000610"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN1974272.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20494..20859
                     /locus_tag="U4A80_000611"
     CDS             20494..20859
                     /locus_tag="U4A80_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974273.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20861..21313
                     /locus_tag="U4A80_000612"
     CDS             20861..21313
                     /locus_tag="U4A80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974274.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21927..23237
                     /locus_tag="U4A80_000613"
     CDS             21927..23237
                     /locus_tag="U4A80_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN1974275.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23260..23571
                     /locus_tag="U4A80_000614"
     CDS             23260..23571
                     /locus_tag="U4A80_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974276.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23932..25188
                     /locus_tag="U4A80_000615"
     CDS             23932..25188
                     /locus_tag="U4A80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1974277.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25185..25730
                     /locus_tag="U4A80_000616"
     CDS             25185..25730
                     /locus_tag="U4A80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974278.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGAIV"
     gene            25731..26231
                     /locus_tag="U4A80_000617"
     CDS             25731..26231
                     /locus_tag="U4A80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974279.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26312..26868
                     /locus_tag="U4A80_000618"
                     /pseudo
     CDS             26312..26868
                     /locus_tag="U4A80_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26888..27232
                     /locus_tag="U4A80_000619"
     CDS             26888..27232
                     /locus_tag="U4A80_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974280.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27251..28162
                     /locus_tag="U4A80_000620"
     CDS             27251..28162
                     /locus_tag="U4A80_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1974281.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28241..29233
                     /locus_tag="U4A80_000621"
     CDS             28241..29233
                     /locus_tag="U4A80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1974282.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29230..29448
                     /locus_tag="U4A80_000622"
     CDS             29230..29448
                     /locus_tag="U4A80_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974283.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29461..29871
                     /locus_tag="U4A80_000623"
     CDS             29461..29871
                     /locus_tag="U4A80_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974284.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29923..32451
                     /locus_tag="U4A80_000624"
     CDS             29923..32451
                     /locus_tag="U4A80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1974285.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32467..34677
                     /locus_tag="U4A80_000625"
     CDS             32467..34677
                     /locus_tag="U4A80_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974286.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34685..35776
                     /locus_tag="U4A80_000626"
     CDS             34685..35776
                     /locus_tag="U4A80_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1974287.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35797..36339
                     /locus_tag="U4A80_000627"
     CDS             35797..36339
                     /locus_tag="U4A80_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974288.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36534..36791
                     /locus_tag="U4A80_000628"
     CDS             36534..36791
                     /locus_tag="U4A80_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974289.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36805..37137
                     /locus_tag="U4A80_000629"
     CDS             36805..37137
                     /locus_tag="U4A80_000629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974290.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37182..37730
                     /locus_tag="U4A80_000630"
     CDS             37182..37730
                     /locus_tag="U4A80_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1974291.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37737..>38053
                     /locus_tag="U4A80_000631"
     CDS             37737..>38053
                     /locus_tag="U4A80_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974292.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFC"
BASE COUNT        12917 a         5987 c         8101 g        11048 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga ttgagattct catcggtaaa ctaattgcat acagagatat gaataagtta
    13381 cttaacaaag agttagctga taatatagga attagtgaag tattactcag ttatgtgtta
    13441 aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt
    13501 aatgaaaaag aaattttgaa ggagattaat cgtgatagtg agatatttag tttatcaaaa
    13561 gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg
    13621 cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttat aagtagatgg
    13681 gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta tcttggtaag
    13741 gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc aaaagatgaa
    13801 agagttagcg attttaaaaa gataaattct tttagtatgg gattaaggat gaggaaaatt
    13861 aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc ttcaatttca
    13921 aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa tcgaattatc
    13981 caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat ataaaaatag
    14041 tttacgtcga tacttattaa gttatcgacg ttttttatta tagaaaaaat ttataaaagg
    14101 aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt actggcagct
    14161 tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa gtaatacaag
    14221 aactaactgt tatgaataac agctagataa aatgggctat gtatgttatc caacaattta
    14281 gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt attgatttgt
    14341 agtgtttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc tgcagcgtca
    14401 ggatgggaat ccataccatg gagattcgga gatctgtgag ggaatactta cgtggttaat
    14461 atgtcagttg tgttggacgt agtaatggtc atagtgggta gattgactga attcttatat
    14521 gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga tcttttctgg
    14581 aaaagtaatc ggataatgcg agattcgtac ggtctttttt aaatttctct aaaaaaaacg
    14641 gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa
    14701 tgattctatt tatcttttta catctatcgt tttttcaagt agccgcaaat tatatcagca
    14761 ttcaacaatt tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt
    14821 gaagataaaa aaagaatcag gttttactga gtttttcttc aaaatttgtg ttattaggtt
    14881 aggacaaaga taagaagaag tatgaagatt taaaaaagga atgaggaaaa gatagtaatt
    14941 tccgattatt ggagtttttt tatttataaa gaagaagcga ataagttgtg attcctagat
    15001 ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt tttagtgttt aatacacgag
    15061 gacaaagttt cttaggtatt gaaggcacac gtgtaacggt tgatgggtgg tgtgattgtt
    15121 tcgaggttgg aaaacatgtt gaacttatgg catttactga gctaaaagat aagaacaacg
    15181 tggaattttt cgagagtgat attggttggg acgatcatga agaagtttat ggaaaattta
    15241 ttttgcggaa gggaaattct tatacgaatg gggaagcata agtagcgatt tgttagagat
    15301 cattgatagg attgaaattg tcgggaatat ttatgagaat ctagagttat ttgaggtgat
    15361 agaatgagtg atggttttga acaagtatca ataattaaaa atcaagttcg ggagattctt
    15421 agaaaaaaag gctatttggt agatagttat tttgaaggag attacgaaac atgggtaggt
    15481 gtctatgctc gtccagagaa taaacctacc tatttagatc ctacgacgtc agaagatgcc
    15541 tatttacaaa ataggtatcg agtagatggt tttaagcaag actttgcgga gtggtttgaa
    15601 tgggaaattg aaaacggaga agtaaaagaa gaataagagt acttgttgta tcaaaataag
    15661 ccagtagaac aaccaaacca gtagctagta atgacaataa tactgaggaa tcggatcatt
    15721 atggtatgat tgatatttct cttccagaag atcttaagcc agatcctgta acaattggca
    15781 atagtgtcat gatttttgat tcattagatg aagctatgtc atatggttgg aaagaaatta
    15841 aggatgaagg tagtaattgg tatggcatga gatttggtga tggtgaagtt gccaaaaaag
    15901 ctggtattcc aattgggaaa tggacagttg atttccacga agcaggttct ttttttgttc
    15961 tgtaattata gtgggagggt aaaatcattg atgaaatgtg ttcagataag agcgccagat
    16021 aaaaatagaa gagacagttc acgtcgttat cacttttggt aacggcgttt tgttttatta
    16081 tataaaattc tcaggaaaag gaggaattct tgtgtatgca aaaacgaaaa tgaattggtt
    16141 gaaagcactc gcattggtgg ctttttctca attctgtttc ttggtgggaa ctgctcaagc
    16201 acatgcagaa gaaacgacgg ctatacaacc tacgatggaa gccattgccc aagtcgcttc
    16261 tgaagtgaca gaagtccaac cagcagaagc cggggtccca gctggtgaag ccactgttga
    16321 acaggcccct gtgacagaag cgccaacggt caattccaaa acgactctcg atcagacgat
    16381 tggggaaaca caacacccag ggaatagtcc tggtacttca caaccagttg aagaaactgc
    16441 ctcaattaca acccctgctt cagaaacgtc cactctagaa ggagaaaaag ctgccgaagc
    16501 aacgccttct gtgcagccag agtcttctga gaaacaagcg gaagaaaaga aacgaataga
    16561 agaaacagtg gaacaaccag cggctactgc tgacaaagaa ggaaaaaatg aggaaaacgt
    16621 tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa ccaacgaaac caaacaaaga
    16681 atctacggct agtattgaaa atcaaaagaa aaaagagcag aacattccaa aaacaggtac
    16741 acctacactg ttagtaacag taccaaccaa tcgttcggtt cgtgttcgcc gtgcaaccaa
    16801 tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa aatattggtg ttacttctag
    16861 tccgaacaat ctaaacttca ctttaaatta tatggttcgt tttgagatta atggacaagt
    16921 ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt caagtttata cagctactgc
    16981 tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa acaagcttga tctcttattt
    17041 aggatatgtc acaaataaag acaaatccga tgaacaatac attgcggcac aatttatgat
    17101 ttgggaagct ttgggcacaa aaatcccaat caaaaatatc cgtgttgatt atgatcgtcg
    17161 aaaagcagaa attcaacgtt tgattaataa ctttggtgaa aaagcaactt ttaataatca
    17221 aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag aataaaaatg attttttgaa
    17281 atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta tctattcacg gcaatgaagt
    17341 atggattaca gtcacaaaag aggcaccgga taaagcaaaa attcaattga ataaaattaa
    17401 agttcaaggt gcgccattag cctatcgtcg tccaggagct caaacaattg gtgtgttaca
    17461 tccaacagaa cctaatgcgt ctgttattaa tttacaaatt ctaaaacaag gaaacattcg
    17521 tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa ggtgcagctt ctttagaaaa
    17581 tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt aaagaaatta ccttgaaaaa
    17641 aattaacggc aaagtacagg cagaaattaa agatcttgat cctggcaaat atgttttaaa
    17701 agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa gcaattgaaa ttgttgttga
    17761 acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg atcaaaggaa ccattgattt
    17821 agtgaaagtt gctaataaag atttagtcga ttcaacgaat ccagataaca aaccaaaatt
    17881 agctggcatt gaaatcactt tgacgtcaaa aacaactggc aaagtggtga aaaccgttgt
    17941 gacagacaat gatggttatg ctacttttgg taaaaatacc gttgtctttg atacgtacat
    18001 tctttctgaa accaaaggaa aagaagggta taaactattt gaaccacttg aagtcacaat
    18061 ttctgaaaat gggcaaactt tccattatgt tttagaagat aaagtaattg aacaacgtct
    18121 taaagtggtg aaagtggatc aagaaacagg aaaagtgatt ccacttgcta atacagaatt
    18181 caagattttt gatactttag ccaatcgtta tgtcacaatg gagattccaa atgatacaga
    18241 aacaacggat atctttaaaa ccaatgaaaa aggctttttc ctaactaacg gtacacttac
    18301 ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc cccgtaaatt acgttttgaa
    18361 tcaaaaccca ttagtgttct cgattaccaa tgatacagaa ccgatcaaag tgatccattt
    18421 tgcgaaccgt caagcacgga aaagtgtaaa attattcaag tatgaagaat ggaacaagaa
    18481 gaaaacgccg ctcgcaggtg tgaactttac cttgtataac ggtaaaaatc aagcaattgg
    18541 tgaatatacc actgatcaaa acggggaaat tttagtgaaa ggtctagttg ccgatgacta
    18601 ttacttcttg gaaaatgccc cattagaaag tttccaacca aatacagata aacaagcatt
    18661 cacggttgca ttcaaacaaa atgcgccaga tgatggggaa acgttagtgg tttctgtacg
    18721 taattacttg attccaccaa aactagaaac acaagcgtca aataaagccg atggtgaaaa
    18781 agtggttgat ccattagaaa cgatcgtcat taaagacagt gtttcttata ccaacttgtt
    18841 tgtcggaaaa gaatacacca tttcaggagt attgatgaac aaggcaacga atgcgccaat
    18901 tttacaagat ggcaaagaaa tcacaagtac cttggccttt attgcagaca aagtcaatgg
    18961 cacaaaagac cttgaatttg tggttaatgc tagtgttctg aaaggtcaaa cgatcgttgt
    19021 ttttgaaaaa cttttccgtg atggcattga agttgcagca catgcggata tcaacgatga
    19081 agcccaaagt gtgaaagtga ataatccaac cgttcatacc acggctgtct ttgaagacgg
    19141 attgacagtc gcagacccaa caggattgtt taccttaaac gataccgttg aaattaccga
    19201 gattcttcca ggaaaagcat acttggtcaa agggatctta atggataaag caaccggtga
    19261 acccttgctt gtgcatggaa atacggtaga aacgagtgtt ggctttattg ccaaagcaga
    19321 aaatgaaacg gtgatcgtac catttacctt tgatttgaca ggtataaatg gtcgtgaaat
    19381 cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta gcaaaccata aagacatcaa
    19441 cgacaaaggg caaacggtac gagtaactaa cccaagtgta cgtacaacgg ctacacataa
    19501 acccactggc ttgaaagagg ttaatccatt aacgaaagtc accattacag acgctgtgaa
    19561 ataccacgat ttggtcgttg gaaaaacata cacagtcaac ggtgttctaa tggacaaagt
    19621 aacggaaaaa ccagtgctag tagatggaaa agaagtcaca agtaccttaa cgtttgtggc
    19681 agaaacaacg gatggtacag tcaatctaga ctttattcta gacgcacgtg cgttgcgagg
    19741 caaagaagtc gtggtctttg aagatctgtt tagagaaggc gtattattgg cttctcacgc
    19801 agatattacc gacaatgacc aaaccatcaa aatcagtaat ccaaccatta cgacgaaagc
    19861 gacgaataaa aatggcggaa aagagatctt ggccttgcca aatcaaactg tgcttgaatg
    19921 ggtcaaagta gacggtcttg cgattggtca aatctatcgc ttaattgttc aaggctactt
    19981 aacaccagac ggcacaccat ttgaaggcac tcatgcggaa aaagtctttg cggccgataa
    20041 agaaatgatg gaattcttat ttgaattctt agtggacggt cgcaatcttg caggaaaagg
    20101 cttgagtttt gcggaatggc tccaagcgaa aacggaagat gagaaagaaa cgtttgaaga
    20161 agtggcgaaa cacaatgtag atctaacaaa caaagatcaa acggttcaaa ttgtggaagc
    20221 gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt aaaccagcta ctgttgtacc
    20281 aatgttgcca aaaacaggtg aacaaacaag tagtatactt atggttcttg gattagtagt
    20341 attagtcatt gctttaggta taacacttta taaagtacgt aaagccaaag attagtcatg
    20401 aggaacaagc ggttaggatt tttaaccgct tgtttgattg taatgtagaa atatttagaa
    20461 tttattttgg tttttaagag gaggaaaaac tttatggcag aaaaagcatt gcgtatgaag
    20521 aaagagtttt taggtcaagt atttttattg ggttcacggg aacctaatcc accaaaggaa
    20581 attaatggtg tgtctgggcg atatgttcaa ttacaagcag agtgttcgcg agaagaacag
    20641 cctgcttttt ttgtttatct cacagctgaa gaggataaaa gattacgatt accagaaaaa
    20701 gaggtgcgta tttcatttga agaagcttat tttgtttttc gtaaagaacg agtgaatagt
    20761 cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa ctggctatca agatgttggt
    20821 gtctttttaa ctggtgtaaa aattggggag gttttgtaag atgggattaa aatttccaaa
    20881 taattatgac attacagaag cattagatga aacttgttta ggagccgtac gctttggcgc
    20941 attagaagag tctgatcgtg tatttgataa tgaaacaggc gattatacgg gagaaattcg
    21001 atcacagcga gcacaattag tttttggaaa tccagatgaa ccaaagattg cacgacgagt
    21061 taaatttcca gcagatgtag atttgactgg ttttaaacga cgtgatcctg tgaaattaat
    21121 tgatccagaa tatcatgtgg tatatattcc agcacaagat ggcaatcgtg aatattcaga
    21181 tttagtgatt tatgcgaaag gattagagaa agtagaagct ccaacaaata atcctaatat
    21241 gaaacaatca caagaaaaag gtttagatca aaaacaacca agtgttgatt ctaaggaaaa
    21301 agagaaaaaa taaggttttt ttgaaagaat aggaaggagt cttcctattc tttttatcta
    21361 agatagaagg aaggggagat tttgaattca gataaaacca atcaaatact ttgagagatt
    21421 gtgcttgtta tcaaaatatc caacaattga ttgagtattt tggagacaat ccaacgattt
    21481 ttgtacaaac aaatgcatat atttgtgcgg ttattgggca gtgtatgttt ttacgtgatc
    21541 ttgttcgttt ttcattaccg taccagtgga aacaagatca tcaggaagtg atttggattc
    21601 gtatagtacg aatatgtaat atgtgtgcac acccttataa aaatcgatta aatctttttc
    21661 gattatgttt ttttactaaa tgtgatattc ctgtattagt tgctttttgt caaaagtttt
    21721 tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca attgaaagct cattttttca
    21781 agtatcgtgg tcggcgaatt cgctatttca atcgttattt atggttttgc tattatggtt
    21841 tctttttgtt tccatttttg attctgttag gttggtttag ttggaaagta ttttatccac
    21901 gtttaagtac gttttcgaat tggcaaatgt atcttattcc tggaataagt attgtgttgt
    21961 atttgttgct ggttagtggg ttaattttag ggttgatgca ttggtcgcga ctaaaagaag
    22021 gatattttgc ctctgtttat tggcgtcagt tacttgttcg aatgttaatt gacaatcggt
    22081 ttgtttatac aaagaaacaa gttagtgaga aacaaacaag ggaaaaaatg agattaccaa
    22141 aagtttattt ttatcaaaag aaacaagaat tagtggtttc ttttccttta gatggtgggc
    22201 gttttcacga acgattttta aaaatgggtc atttgttatc tgaagtattt ttgcgtgatt
    22261 taattcgtga agaacgagaa aaagcccgaa tcaattatgt attcctttca gattctggtc
    22321 ggattcctat tgatcaatgt atagcagaaa atggacgtat tcatttaatg aaaactttgg
    22381 attgggtata tgatcaatca ccaaatatgt tgattgctgg tgcaatcggt ggtgggaaaa
    22441 cctatttatt gtattcttta attcaagctt gtttaagtgt tgggacagta gatatttgcg
    22501 atggaaaagc ggcagattta gcagcattag gtgacgtcga tgtattcaaa ggacatgtgt
    22561 tttacaaaat aaatgaagaa atgattattt gtttacgaaa tgcattaaaa gaaatgaatc
    22621 aacggtatgc ctatatgaaa accatgaaag atcctaaata tgaaccaggg aaaaattatg
    22681 cttattatgg gttaccaccg cattttatct tttttgatga atgggcagct ttttatggtt
    22741 cgttggatat ggaagcgaga aatagagtcg ataaattgat tcagccatta gtattaaagg
    22801 gtcgtcaagc agggatgtac attattttag caatgcaacg accagatgca gaatattttc
    22861 caagtggtgt tcgggataat ttaacattcc gtatttctgt tggtcgattg tctcccattg
    22921 gttatttaat gacttttgga gaagatttta aaaataagcc attttacaac aaagatcaac
    22981 ctggtcgtgg atatgctttt ccaggatttg atgtacctgt agagtttttt gcgccatttg
    23041 tttacaaaga ttatcacttt atcaaagaat ttgagaaatt tactgaaatg attgtctatg
    23101 attttacagt aaaagaggat agtcaagatc aaacgatggg agatgataat cagttgttac
    23161 aagaacaact agagcgccaa atgtatttag caaaagatcc aaaaggaaca agtttttctg
    23221 atgcgcatat tttgtaatca actaaggagg tgaagttaaa tggcagagag ttgttattat
    23281 agagtagcta cagcaatacg aatgattaat ccttctcttt cttccagaac gttttatgat
    23341 tggttgaatc gtattgaaca agtaactgat taccgctttt tgcgcaaaga acgtgttttt
    23401 acaggaaaag ttattaatca agtattgcta acgaaaaaag atatagaacg attgactcga
    23461 ttgtatcatt atcgagtaga tttagaagag gatttaactt tgtcaattta tcgcgttttt
    23521 tctcctgaaa aatatagtga aataacaaaa ttagatcatt taattttgta gttattatct
    23581 atcgggaggg ctctagacgt agtctggtag ccgcactctg cctggcggtg cgcattaagg
    23641 ctgcgaagca tgggcctata atgcgcactc gctagcagcg tgtgcagctc acaaagaggt
    23701 cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga tatataatat ggggggcaga
    23761 aataacataa cacatagaaa aaagttttat aaaccctttt gtatcaagtg attatctaag
    23821 gtcagcagtt gtactacttt tcccaaaaaa cgagggggac tagttgatat atcggtgttt
    23881 ctaaagggtt aaaaaaaatc gattttgtta ttttataaaa ggagcgagaa aatgacaccg
    23941 attgagctaa aaagctacag aaagaagtta agtgcaattc ataatataag tggagataaa
    24001 aagccgctat cacaagcaag atttgcgcaa ttgatgggct gctcaaaaga ttacattaga
    24061 aagatggaac aagggaaaag aaaaatccca gaggattttg aacaacgaat caaggaacgc
    24121 tttcgattgt atatgaaaag cggaatagat cttcgagcaa tgattgatta tttgcgtctt
    24181 tccttttttg ggacagacta caaaacagtg attcaacgtg tattacgaat ggatctgaca
    24241 gaatttgaaa cacgtccaac gggcttgtat ctttttgact gtctttctgt tcgtgggaat
    24301 atctgggtgt tctggcattc aaaacgaact gtaacagaaa atgtacttgt gcaattttca
    24361 ggacaaggtt gccgggaata tgaggaagtc atgcgtgaac aacaaacaga ttggcgaaca
    24421 tggctatatt ccatttggaa aaatcaagga aagttggaag ctggttatac acgtgtgcaa
    24481 tgtagccgat tcgatattgc gttagatgaa ctttggggat tggaaacgga agaacatttc
    24541 gatctctttg aattactaga aaaacaaaaa gcaggtttat tagaaatgga ctttaagaga
    24601 ttccagaata ttggcggttg ttttttagat gatggctatt ttcgaagtga aggattatct
    24661 ttgtattttg gatcgcgaaa gagtcctctc ttttttaatt tttatgagaa acgttttgaa
    24721 attgcgaacc gagaaaaaat ttcagaatta gaagcattaa cgaaatatgg tatttataat
    24781 cgatatgagt tacggttagc caatgagaaa gcaacacaag cagtagaagc atttatttta
    24841 ggtgattcac cgaaacgttt aggagagatt ggcgttggtt taattaatgc aaaacttatt
    24901 gtttgggata gactggaaag tgatgcacgg attgttgata aacgatgggc aaaattattt
    24961 cgttcgatgg aagaattgga ttttacagta aagccagaaa aattctcatt agatcgttca
    25021 gaacgctggt ttttgaaaca agtagctccg acattaaagt tgttacaaga agcagatcgt
    25081 attttaggaa gagactttgt acaagaaggg attgataatg ccgaactaaa tgacgagcaa
    25141 caaaactatc tagaatggat gggaacgtca ggagggaaaa ttgcatgaat gcatatacga
    25201 caacagaatg gctgcgcctg ttggatttaa aggctgcaat cgaagcatta aatgaaaaga
    25261 tggttgattt gtcttatttt cgttttcgag ttccttatat tgaacaagcg gtaaaagctg
    25321 gacgttatca ggaaaaagag aactggcaag aaattgcacg cttattagaa gttcgtaagg
    25381 gatacgaaca agaactcgaa gagttggaat ttagccgaag gaaaggaagg ctagaattca
    25441 ttcgctttta tcgattttca ttgccaatac ctgcgatttt agcggttaaa aaaggctgcg
    25501 ataaaatgaa gatatatgaa aactgtgtgg cagcacttag cagcgaaaaa cctcttatgg
    25561 aagaaatttc attggtgact gtcacttggg aaatgagtcg ccagccaaca gaagaacaga
    25621 catatctttc tttagaagag attgagatag aattagagga aattggtcgc tatgccactt
    25681 gttctaccta ttgtggatcg gtcattagta ttgctggtgc gattgtctaa atgaaaaaaa
    25741 ttatgattta tgttggtgcg tattggtcac gtgatccaac agtattagaa aatccagagg
    25801 cggtacacta ttgcttgcgc caattattct atctgtataa agaacgatta gaatcgttga
    25861 ttcgtcagtt accatatact gacagacgtt tagatgaact tcttctaagg tatccagcaa
    25921 tgtataaacg acgaaaaaat agattgttac cagaagagta tcccattgaa aaacatgaac
    25981 ttgaagggcg atttgtggct tatttctatg atgatgttcg tatgcgctta gtagaacaac
    26041 gaatggaaat agaaaatgat cgctactatt ttatcaatta ttgtaagaag cgtaagtatc
    26101 aagtaactga tgatttttat gattgtattt tacaagatgg ggaagtgatt ctatccaaaa
    26161 ttgctccaag ttttcgtgaa cttgtgccga ttgatttttt gaaaaaatgt catttgcgga
    26221 ttcttccgta aaaaagagct ataaaaggaa agaaaagtag aagccaaatc ggtttctgct
    26281 tttttatttt ttttaggaag gagaagatgg tatgtcactg gaatttttag gagagccaat
    26341 tgaaaaggaa gagttcgttt cgcattatat ggatttgttt attcaggtga taaaaaactt
    26401 tgtgcgattg atgaatttta tccacgcgaa caagactatc tagccgctga acgaataaaa
    26461 atcagtttat tatgggaaaa attcacagat gaaaataacc gaccgccaga ttatcgctat
    26521 ttatcagatt gtttaacgaa taatgtgctt tctaaagaat acctatttca caaacgtgag
    26581 aaaaatcaaa tgtcggttga acatttttgt gattgttata ttgcattatt gcgtagtcaa
    26641 cagttattaa cagtagaacg atatggtgca gagtttggtt atgtcatgca agctgagcgt
    26701 catagagcta caagatgcta tgaaaaagaa gatcaagtga ttgatggcta tgaagaatta
    26761 tgtattcgaa acaatcgcta tttgcaacaa cgtttatatg aacaattgat gatggatttt
    26821 tcaaatctct atagagggta tgaatgttcg atgattcaag aaaggtagaa gaaaaggggg
    26881 aatttttatg tggcgattat tatttttggt ggtatgtggt atggcattgt tctatgtgta
    26941 tcgtgtctat ctctatccac gaatggaacg atggacaagt tggtatagag atcctttaaa
    27001 acaacgaaaa ggattgaagc ggtatcgtta tgaattactt gttgaatttt cggatgggcg
    27061 acaagcacag tattattttt ttgcaaacta tccaaaatac aacaccagtg aactattgat
    27121 tcaattattc aaaaataatc catatgttgg gttagaagaa aacggacggg agttctttta
    27181 tcgttcagat aatattagtg cggcgtctat gaacaaaaca cgaattgcat aaaaaattgg
    27241 aggaataatc atgaaaaaaa caacattagc agtcgtaaca acactaggaa tttcattatt
    27301 ttcgtttcaa ttagggagtt cattggttta tgcagatgaa actgtagaaa cacctaagtc
    27361 agaaattgta gaaacattgc caggggaagg attaccagat gttgagacag agctaaatgg
    27421 aggaacaacg gaaaaaccag cagaacctga acaattgcag tcagagaaac ctgaaaaaga
    27481 gccagtgaca ccagaaccag agtttcctga atcaacttta ccgactgatt cgatcgaaga
    27541 agaaggtgtg catagttcaa caacaacgga ttcaagtata gtcacaactc cggaacaatc
    27601 aggaacaacc agttcttctg aaaagaatac tgattcgtca agtacggaac agccaacgat
    27661 gtcgtctacc agtgaagaac aaaaagaaga accgaaacaa cctgttgaaa aatcagaagc
    27721 aattccaaaa gaaaataaac ctattgttcc agcaaaagaa gtcacagtat cggtaacccc
    27781 aagcggtgaa attacgacga atacttctca aggaatgagt gtaccgattg tcactagtaa
    27841 tgtagaggaa ttgacccata ttccaacacc aaccacccca ttaaaagtag caggtgatca
    27901 aacaattgtt ggtgtaaaag atggtattcc attaatccaa gatagtgaag ggaatttggt
    27961 aaaggatttg tcgattcctg taaagaaact tccaagtggc aatattgaaa tcaaaacagc
    28021 agatggaaaa acaaaagtct taccaaagac tggtgaagaa atacatgttg cactttctgt
    28081 tctaggagga gtgttgacaa gtattgctgg atttgttgga tataaaagaa agagaaaagg
    28141 aatagaaaat gagggtaatt gaatggacta tttttcagaa aaacaagctt atcgttgtca
    28201 atcgtacgta ctagtaaaaa catactagaa ggagtagctt atgaaaaaac gacaactacg
    28261 gcggaaagaa aaagtttata cagagaaagt accgaagcaa aagaaaatcc attcaaaaaa
    28321 agcagtgaaa tggatttggt gtttggtttt atttctttgt ttttcagggg gcttggcatt
    28381 ttttcaatcc atgaaaacga aggttcaagt gtcgtttttg aaagatgaaa tccaacaatt
    28441 acaaaagaaa gaagtacaaa gtcggtcaga attaacgtac acacctgaaa ttgaatcctt
    28501 tatgaatcgt tttgttgctt tgtatatgaa tacattcgat aatcatgaac aagaacaaca
    28561 acgtcaagaa acattaaaaa aagagtttta tgcacaaaac tttattcaag aaccaggaag
    28621 tgtttcaggt gttcgaaaat taactgctgc ttcgtttatt ggattaaaag aaattgaaac
    28681 cattccaact gctagttata aagtaactta tgatattcgc tcgatgaatc cagagagtaa
    28741 gaaagaaaca agtgtgacaa tcacacaagt actcaatctt cccttttcta tttcaaacaa
    28801 gggctgtcgt attattgctt atccttattt tacggcacta ccaaagaata cagaaaaaca
    28861 acgaatgatt acttatgatg acacattata cgaaacggtt gatgcgtcgg aagcgaaaga
    28921 tgttgaagga tttgtggaag atttcttaga taagtatgcg aaaaacaatg caaaagatat
    28981 gtcatacatg atgaaagaac ctgaaggact caatcaaaca gctaaagttg atcaagtaga
    29041 gatgaaagta ttgcgttata aagaaacgaa aaaacctttt attgtaaaag caacggtcgt
    29101 ctttcgagat ggtgatacgt cacttttaca aaaagaacaa atgacgattg tcattacgaa
    29161 aaaaacaaat cagtattatg ttgaaaaatt aacacataat tggttggata ggaaaggaga
    29221 agaacggaaa tgacactgaa gggaattgta gattttatat taacgcaagg tggctatttt
    29281 ttaataatgg tggttgttta tctgggattg cgaaatttta tgaaatcaaa agtaggaaat
    29341 gcattaatat ctatcgcagc aggtgccgca gtttatttct tcttaaatga tccaacacgt
    29401 gtatttgatg cgtttggtaa aatcattgaa aaattattta tggattagga ggatagtgaa
    29461 gtggattggg aaccttatga ttattcgatt gtttatgcgg aaccttatcg cgtacaacag
    29521 attacaagca attggcgtct tccatttcca gttgcattag tcaatgcagc aacttttttg
    29581 ttggtcgtaa tctttgaata tttattttta cgttcattta ttggttgggt catttctttt
    29641 tttccaccag caggagttgt tttttatata ggactcccct atttatgtgt gttagcactg
    29701 gcaaaactaa aacctgatgg aaaaaaagtg catttttatt tatgggattg gttttgttat
    29761 atgattcgta tgatgaatgg tgattgtgtg tattataact gggacaaggt atcaaacaat
    29821 gcattggaag aattactttt tgtgaataag tatgtttatt tagaggatta gaaaaaggga
    29881 caatcaactg gttgttcctt ttttattggg aggtgaaatt cattggtgct tgtaaaagaa
    29941 ccagtaaaag cattcaagaa aaattgtttg ttaaatggag aggattgtat ctgggcctac
    30001 tatcatgttt ttccacgaac aatttcaaaa aacacaccaa aaaagacaca ggaaataaaa
    30061 gaaatgatgg ctcgtttact gttgctttta gcagattatg aagatgtgga gttggtgatg
    30121 tatccacgaa caatgaattt acggacgaag tttgacacat taggaaaggg attttctccc
    30181 gatagtcaag caattggtgc gtattatgca aaagaaacag tgacattgtt ggaacgagaa
    30241 ggtcaagtct atcgtccttc atttatttta ggaatcaaag tcaaagaaaa agcaacggtg
    30301 gagagttttc atgatgtaat aaatctaatc tggttagatt tagcaggaag tttagcacgc
    30361 ttatttggat tagaagtaga tgatttggaa actttgtttt ctgaaagtga tccttatgta
    30421 aaagaagtga cacaattact ttatagcttt tctattcgtc ccttagtaaa aaaagagctg
    30481 ttgtatctca atcgtctgca atatattcga ggaatggctc acaaagtaga agaacaaaat
    30541 aattgggaag tactgaaaga caatcaagga acgtatgtac gtataccgaa ggaacataaa
    30601 ggccttcttc aattagaaga tgagttaggg agtagttatt tagcattgtt gcctattggg
    30661 gaaacacctg aaaatatgag aaattgtgat ctatttcaat ttgctcagga tctttctttc
    30721 ccagcagaat tgcgaatgaa aattcactat ccacgcttga aaggacaggg aggtattggt
    30781 ttaaaactga attggctatc ggaacggttc aaaacagagc ataaggaatt ggttcaaaat
    30841 gatgaagaac ctagtgaacg cttattaact gttcgaggat tagtaaaaca tttaaagcaa
    30901 cgagtagata atgggcaacc tgtcgtcgat tggatcgcat gttttattgt atacggtcaa
    30961 acggttgaag aagttcgtca aaatagtgca acggttattc aacaacttca tacgattatc
    31021 catatccacc gtgcaaaaaa tgatcaacga gaattgttct ataaatgttt attagggcaa
    31081 ccattgggac ggcagttaca ttggaagcac cgaacaactg tagaagcagt tggcgaatgg
    31141 ttgttttcag cagtatcaga attaggtatg gaatccggct ggtatattgg tcggcaagat
    31201 acattagggg atggtggttc tcgaggagaa aaaacaccat tagaagaatt aatttatgcg
    31261 tcaaatcgtt ttgtcttttt gaatcttcta gccattgcgg aaggaatcaa aggtgcgttg
    31321 tttgatgcgc ctcatgtagc agtaactgga aaaaccggta aagggaagac ctttttagtt
    31381 ggcttgctct ttttgtatag ttcgtttatg aatgtacaaa gtttatttgt ggatcccaaa
    31441 ggagaaaagc gcaaatggtt tacgaaactc attcaagacc cgtattatca gaaacattac
    31501 cctttgtttg tagaacattt gcaaaaaatg agttatgtca cgttagatgc tacaaatccc
    31561 cgtaattatg gtgtattaga tccaattgtt tgtttatccc gagtagatgc gaaacaagta
    31621 gcccaggata tgattgatga gttaagtccg ttaggtacca atcacctttt aaaaggggcg
    31681 gtattaaagg gaatccaaga agttttagtt caaaaagaag ccggacaaca agttggcttg
    31741 atgcatgtgg tcgactattt agaacaaatg gaacaaaaag aggttcgaga atatggtgag
    31801 tttttacgct tgacggtaga agattcgatt ttacgtttag gattttctta tggagaaaac
    31861 ccgggattag actttaatgc caaaacaacg attttagaga tccaagattt gaaattacca
    31921 aatgacaatg tccgaccaga actatataca gatgcggatc gaaagtcttt gtgtttgatg
    31981 attagtttag gtcgtttttg tgaaatgttt ggaaaacgag atagtagtaa gaaaacagcg
    32041 atttatttta cagaagcatg ggtatttaat aattcgaacg caggacgtag tattattgca
    32101 gcaatggcac gtgtggggcg ttcccagatg aatcaattag tgttagatac acaatttatt
    32161 ggtgacttag gttcagataa agaaaaaggg aattttggtg tcgtctttgc ttttgatgaa
    32221 gacagtgagc gagaaaagat tttgaatcat ttacgtttgg aagtaaatga agcgaactta
    32281 gctgaaatga gccacatgat taaaggacag tgttggatgt tagatcccta tggacgtgta
    32341 ggaaaactca atgtacacag tttgtttgaa gaaatgaccg ctgcattaca aacaacggaa
    32401 aaaacaagtc actctcaagt agaagaacag gatacgttaa tcatgcaata gaaatgaggt
    32461 gaaacaatgg cttatataaa gcataaggaa aagaaatggt gcgttttgtt ttttttaacg
    32521 attggattcg gactgctttt tgttggtgga ttggtgtatg cggcagaaaa gcaggtggat
    32581 caatcgatct atgagattgg gaaatttcat attgatacct tttcttctta tttatctgat
    32641 agtgaatttt cattaggcaa aatgttattt ggggcaaatg gcacagattt tctaaaaatg
    32701 atgacgaata tgttctttag tttaagtaaa ttgatttggc aaggccttga ttttgtcatt
    32761 gagaagctct atgaaggagc tgcaatgaac gagttaattc accagttttt cactttttcg
    32821 gatactattt attcaaaaat atacgatcct attggtgcgg ggattgtcgt cttgtatgtg
    32881 ttatatctat ttactcttcg tatatttaga gggaaacatt acgcaaaaat ggtgtttatt
    32941 cgatttctct tcgttgtcat tttttcaatg gtttggtttg gctatggtag taacgtacct
    33001 agtaaaggag aagcttttac caaagggtta aatgatttgt ccgtggaggt agaaggcttt
    33061 attttttctg ctacaaatag tctagatgaa ttggcgacaa aagatgtcaa tggtgtttta
    33121 gtgccaacga cacaaaagga agccattcaa aaagtgcgcc aactttatta taaagcggct
    33181 gttgttgatc cgtatatatt gttaaattat gggacaacaa atttagaaga attgtatcaa
    33241 gcagagattg aaccacaaga atttctagcc aaggaagcgt caagtgaaga attatctgat
    33301 attgagacaa aagtgaccac tgctgcagga gaagatagag ggaaaaagaa ggcttatcgt
    33361 gtctatattc aaccaactaa agctgtttat aaagcagtcg ttggttctat ggtgccattg
    33421 cttaatctaa gtattggatt accacttgta gtgattggca tgattcgttt tatctttcaa
    33481 atgttggtgt tgtgtttatt acttgtgatt cctttttcat tactcgcttc ttttgttcca
    33541 gtatttgatt atatgctcat gaatacctta aagtcctttt tgggcttgat gtttcaaaaa
    33601 tctttttaca gtgtgattat tttggtggca tttttgatct ataacatagt agacgattta
    33661 attccaatga atactgtttt tggtttttta ggaaatatgt ttgtcaaagc actcctatca
    33721 attttactca ttgtaaaacg gaaagattta ttaaagaaac tagggttagg agctgcagat
    33781 caaactataa acaacacgaa aaacatagca agtcattctt tgtctcaaat gaaacaactg
    33841 cctggtcaac tatcaactgc tggacaaaat ttggcaatca aaggtgcagg attagcaggg
    33901 aatgtgtatc gagcaacgaa tccactcgct tctgcagcga tacatggatt tcaaaagttt
    33961 caacaaaaac gtacaccaca agaatcggta aaagggaggc aagtggttaa tccctttgaa
    34021 ctaggtgggc gttcaaatca agccttgcct tatagtggtc gtcatgttcg ttatcatggg
    34081 gtagagcctg aaatgtcttc taccaaatcg aaaacagagt ggaattctgg tctttcaaat
    34141 ggtttgattc caaattcgtc catgaattca gtaagaccaa aacaacaggt acaatcattg
    34201 gcacaattga gtaagcaaca agggaataat caaagaaaac cattgttaaa taaaggtatc
    34261 gcttttgttg gtcaatcgat tccgttgcct tctggaaaag tacccgtgcc acaattaaca
    34321 gctgctggtt ctctttcaca acagattcgc caagtgaaaa atcaggcgaa aaatgggagt
    34381 tatccagcaa tgcagcagtt attgaaccaa tactctgttc ctaattcgtc ggtagccact
    34441 ctatcaggaa atcgttctgt gcaacaaccg gggcaacgtt cgtctgaaag taaaccgtcg
    34501 atttccgcac caaaatcgtt acaacctgca ttttcttctt tagataagcc aacagagcaa
    34561 tcggaaaaaa tagaaaagga tcctaaaaca ctgccgattt tggacgcaaa accaggaact
    34621 gattttactt taccaaaagg acgaacagtc caaaaaccat tatcgaagaa aggatgagga
    34681 agaaatgaag aaagggtgtc taggttgtct tcttttcttt attcttccct tttttctatt
    34741 gccattcttt ttttcaggag aactttctga gcatggagga gaagctacag gtctagctgt
    34801 cgcttttaat ggggaatata ccgaaggatt accgattttt tcagagatta aaggacgtgg
    34861 tccaatcaca gatgaacatg cgcaagcagc agttggtgca gctgtaaagt atcagttatt
    34921 accgagtgtg attctttctc aattaggcta tgaatcagct tatggacagt cacaatctgg
    34981 aagaaacgat acgaactttt ttgggattac ctggtttcct ggttgtccct acccacaagg
    35041 ttctgcacgt ggaattggtg gttctgaagg tggtttctat atgaagtttc cgaacttaaa
    35101 agcttgttat gactattatg gctttatggt ggcgagccaa agcaacttta atcaatgtgt
    35161 gggcaataaa gatcctggaa gttgtttatt gattctaggt cgtggaggct atgcggcagc
    35221 aggtattacc gaagggagtc cgtattatcg aggagcaatg agtatcataa cgtcctataa
    35281 cttaacggaa tatgatactt ttgcgatcaa gcattggaat agcctaccaa caaaaaaaac
    35341 ttcgcttatt gcaggaaaaa gtaaaggcaa tgcgaaggtt ttggatcgga tcattggacg
    35401 atccgtaaat aatggggaat gttatggtgg tacggcatgg tatgtatctc aattaggtgg
    35461 cccacagtta atgggttctg gacacatgtt tgcgatgtta ataggggaag attataactg
    35521 gacaacctat ggttggcaag taatcatgaa cccaaaacca agtgatttac aagcaggaga
    35581 tgtggtgaat tggcaagcag gtggtgcatt gtctcctgga atctatggtc acaccggaat
    35641 tataacagat gtttcatatg gcggacaaca tttctctagt gtggaacaga atgccgagca
    35701 aggacggatt atggctcggt atcaacgcac gtacaatcaa acaaaaattc gtagtctggt
    35761 tcgtaaggtg aaataagaaa ggcagtgact agaaacgtga aaaaagggat aattttagta
    35821 tttagttggt tatgtttgat aggtcttact ggttgtcatt ggttttcttc tgaaaaacaa
    35881 aaaacacaat tgagaacgac agaagaaaca atcgaagcag taaaagaaac aagtggaact
    35941 cttcaaagta ctgaaagtaa cagtcaaatt ggttcattag aagacgagac agcgcaacaa
    36001 atgcaaatag atgaaatgac aaaaaatttc ttaaaagatt ttgctagaaa atggagtaat
    36061 ttcacagatg tttataaacg caatcaagct gtgagggact atatgacaga tcgttgtatt
    36121 aaagacaata gtattgatgt agacccgcat gtacaaatga aaacaactgg gaaactttac
    36181 caggtgacac aagatataga gaatccggat caatatttgt tatttggaga agaaacaatg
    36241 aataaggtta ctcaatttgt attacttcaa ttgaaagtag atcaagaagc tcaaaagatt
    36301 gatgcgatca agatttatta tgtacggagt gcgtattaaa ataaaaaatg aacactcata
    36361 tttattatac accgaagaaa tagaaaaaaa cagactgttt ttttaagaaa aactatgtca
    36421 ttctttactt gaaaaaagtt gttataggac agaaaggata aaacaaaaac ggaaaaaata
    36481 tgtcttatga ataactataa gaactattct attttttagg agatggatac gagatgaaaa
    36541 aactaattgg ctatagtgtg atgtgtattg gatttgttgg tgtgggattg tttgtatcga
    36601 ctgaatctgc ttctgcggct gaatggtata atcctatctg gtataatccg aacattgaat
    36661 gtcaaaaagg ctatccgtat ggaccgacaa atctgaaaaa tggaaaatgt agagtaaatt
    36721 ggaatggcgt tgtagatagt gtaattactg gttcaatgga ctctgctcta catggcttta
    36781 cgggtcacta gaaggaggaa gaaaatgtct gaagagaaaa aagaaatcgt tgaatcatta
    36841 gtagctttaa aagattctct ttctaagatt gaatatgttg ataggcaaga aattcaaaaa
    36901 ctaattgatg atactattat tgaaatccaa gatgcgcgtt gtgaaggtat aaaaatttct
    36961 gtagctttat ctaaagtaat agaaaaaatg aaccgttcat tagcgtttaa tggattgaag
    37021 ctagatagac aaactagtct gatatgggat catttaaaag acttgtatga caagtctaaa
    37081 agaagtgaac ggactgctgt atcaatctta aaaggccttt ggggaatgaa ttcataaaca
    37141 agatatttaa aaagtaactt attttttatg gaggagaaat tatgaaaaga ataattaaaa
    37201 tatttggtgt tttaggtgtt ttcttattag gtttaagtac ttttgttagt actggaacaa
    37261 gtgtgtatgc cacagaaaaa gtgaatacag ttgatttaga aataaagaat aaattagtag
    37321 atgaattaac tttttatttt gatacagttg gttttttaga cgaacatggt gtataccata
    37381 taaaggattt tggtgctatt cgtgaacgaa tcagtcaaga taacgaacaa ggaagagcag
    37441 caagagtctt ttatgaacat attattgttc ctagaatgga gaaagttgat tggaaacgat
    37501 atgctggatg tgtagccatt aattctcttc ctggaggagg agtggcttgg gctataggta
    37561 cgcagggaga ttttattgaa gcattaaaag cttttaattt tgaaaaagct gcggatttat
    37621 tattaaaaat tgcagaaaaa caattaggaa aagctgcagc taaagagtta gctaaggtaa
    37681 atattgtagc aggaattgca gcagctgctg ctagctgtgc attagagtaa aagtttatga
    37741 aaagtttaga acaagagcat atggctttta aacaagcaat gtttaaagaa aatatctatt
    37801 taaaccataa ctatattcgt gtttctaagg catgttcacc agtattgaat atgcttggag
    37861 gtggaaatgg attatatcat ttactgtttg tcgatgtttg ttggttaatt tttttaccag
    37921 atgaactagt tatagtcaat gaaaaaataa caagtaaaaa tgaagtgttt aattattctt
    37981 taacaagaat taattacaaa gagattacca agttttcact agaaaaagtt cctttttggg
    38041 gagaattttg ttt
//
LOCUS       DASJRQ010000013        36947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJRQ010000013 DASJRQ010000000
VERSION     DASJRQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.013"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="U4A80_000632"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="U4A80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1974293.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="U4A80_000633"
     CDS             complement(1692..2711)
                     /locus_tag="U4A80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1974294.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="U4A80_000634"
     CDS             complement(2733..3740)
                     /locus_tag="U4A80_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1974295.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="U4A80_000635"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="U4A80_000635"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1974296.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="U4A80_000636"
     CDS             complement(4391..6685)
                     /locus_tag="U4A80_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1974297.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="U4A80_000637"
     CDS             6945..9110
                     /locus_tag="U4A80_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1974298.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="U4A80_000638"
     CDS             9414..10247
                     /locus_tag="U4A80_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1974299.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="U4A80_000639"
     CDS             complement(10349..12049)
                     /locus_tag="U4A80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974300.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="U4A80_000640"
     CDS             12556..13716
                     /locus_tag="U4A80_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1974301.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="U4A80_000641"
     CDS             13732..14175
                     /locus_tag="U4A80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974302.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="U4A80_000642"
     CDS             complement(14658..15188)
                     /locus_tag="U4A80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1974303.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="U4A80_000643"
     CDS             15293..15844
                     /locus_tag="U4A80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1974304.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="U4A80_000644"
     CDS             complement(15997..16470)
                     /locus_tag="U4A80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1974305.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="U4A80_000645"
     CDS             16608..18080
                     /locus_tag="U4A80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1974306.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="U4A80_000646"
     CDS             18077..18787
                     /locus_tag="U4A80_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1974307.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="U4A80_000647"
     CDS             19139..19423
                     /locus_tag="U4A80_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1974308.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="U4A80_000648"
     CDS             complement(19740..20123)
                     /locus_tag="U4A80_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1974309.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="U4A80_000649"
     CDS             complement(20125..20709)
                     /locus_tag="U4A80_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1974310.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="U4A80_000650"
     CDS             complement(20756..22231)
                     /locus_tag="U4A80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1974311.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="U4A80_000651"
     CDS             22412..22723
                     /locus_tag="U4A80_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974312.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="U4A80_000652"
     CDS             complement(22838..25174)
                     /locus_tag="U4A80_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974313.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="U4A80_000653"
     CDS             25316..25984
                     /locus_tag="U4A80_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974314.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="U4A80_000654"
     CDS             25987..27030
                     /locus_tag="U4A80_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1974315.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="U4A80_000655"
     CDS             complement(27267..28148)
                     /locus_tag="U4A80_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1974316.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="U4A80_000656"
     CDS             28307..29035
                     /locus_tag="U4A80_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1974317.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="U4A80_000657"
     CDS             29191..29508
                     /locus_tag="U4A80_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1974318.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="U4A80_000658"
     CDS             29501..30109
                     /locus_tag="U4A80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1974319.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="U4A80_000659"
     CDS             30164..30955
                     /locus_tag="U4A80_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1974320.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="U4A80_000660"
     CDS             31093..31581
                     /locus_tag="U4A80_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1974321.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="U4A80_000661"
     CDS             31632..32504
                     /locus_tag="U4A80_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1974322.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="U4A80_000662"
     CDS             32680..33042
                     /locus_tag="U4A80_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1974323.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="U4A80_000663"
     CDS             33051..33803
                     /locus_tag="U4A80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974324.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="U4A80_000664"
     CDS             33817..35619
                     /locus_tag="U4A80_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974325.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="U4A80_000665"
     CDS             35896..36936
                     /locus_tag="U4A80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1974326.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11455 a         6547 c         7633 g        11312 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaa
//
LOCUS       DASJRQ010000014        36740 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJRQ010000014 DASJRQ010000000
VERSION     DASJRQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.014"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..3397
                     /locus_tag="U4A80_000666"
     CDS             119..3397
                     /locus_tag="U4A80_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1974327.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3938..5845)
                     /locus_tag="U4A80_000667"
     CDS             complement(3938..5845)
                     /locus_tag="U4A80_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1974328.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5975..6850
                     /locus_tag="U4A80_000668"
     CDS             5975..6850
                     /locus_tag="U4A80_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1974329.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6930..7904
                     /locus_tag="U4A80_000669"
     CDS             6930..7904
                     /locus_tag="U4A80_000669"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1974330.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8084..9229
                     /locus_tag="U4A80_000670"
     CDS             8084..9229
                     /locus_tag="U4A80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1974331.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9366..9710
                     /locus_tag="U4A80_000671"
     CDS             9366..9710
                     /locus_tag="U4A80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1974332.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9981..11267
                     /gene="efmA"
                     /locus_tag="U4A80_000672"
     CDS             9981..11267
                     /gene="efmA"
                     /locus_tag="U4A80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1974333.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11405..12004)
                     /locus_tag="U4A80_000673"
     CDS             complement(11405..12004)
                     /locus_tag="U4A80_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1974334.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12408..13529
                     /gene="mnmA"
                     /locus_tag="U4A80_000674"
     CDS             12408..13529
                     /gene="mnmA"
                     /locus_tag="U4A80_000674"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1974335.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13775..15196
                     /gene="arcD"
                     /locus_tag="U4A80_000675"
     CDS             13775..15196
                     /gene="arcD"
                     /locus_tag="U4A80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1974336.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15210..16328
                     /locus_tag="U4A80_000676"
     CDS             15210..16328
                     /locus_tag="U4A80_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1974337.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16388..17680)
                     /locus_tag="U4A80_000677"
     CDS             complement(16388..17680)
                     /locus_tag="U4A80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1974338.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17812..18390
                     /locus_tag="U4A80_000678"
     CDS             17812..18390
                     /locus_tag="U4A80_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1974339.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18519..18896
                     /locus_tag="U4A80_000679"
     CDS             18519..18896
                     /locus_tag="U4A80_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1974340.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18829..20901
                     /locus_tag="U4A80_000680"
     CDS             18829..20901
                     /locus_tag="U4A80_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1974341.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21112..22059)
                     /locus_tag="U4A80_000681"
     CDS             complement(21112..22059)
                     /locus_tag="U4A80_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1974342.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22305..22769
                     /locus_tag="U4A80_000682"
     CDS             22305..22769
                     /locus_tag="U4A80_000682"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1974343.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22831..23874)
                     /locus_tag="U4A80_000683"
     CDS             complement(22831..23874)
                     /locus_tag="U4A80_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1974344.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24251..25216)
                     /locus_tag="U4A80_000684"
     CDS             complement(24251..25216)
                     /locus_tag="U4A80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1974345.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25450..26295
                     /locus_tag="U4A80_000685"
     CDS             25450..26295
                     /locus_tag="U4A80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1974346.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26486..27673
                     /locus_tag="U4A80_000686"
     CDS             26486..27673
                     /locus_tag="U4A80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1974347.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27679..28035
                     /locus_tag="U4A80_000687"
     CDS             27679..28035
                     /locus_tag="U4A80_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1974348.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28037..28375
                     /locus_tag="U4A80_000688"
     CDS             28037..28375
                     /locus_tag="U4A80_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1974349.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28451..28707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28770..29804
                     /locus_tag="U4A80_000689"
     CDS             28770..29804
                     /locus_tag="U4A80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974350.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29797..30483
                     /locus_tag="U4A80_000690"
     CDS             29797..30483
                     /locus_tag="U4A80_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974351.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30507..31355
                     /locus_tag="U4A80_000691"
     CDS             30507..31355
                     /locus_tag="U4A80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1974352.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31552..32325
                     /gene="sufC"
                     /locus_tag="U4A80_000692"
     CDS             31552..32325
                     /gene="sufC"
                     /locus_tag="U4A80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1974353.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32338..33624
                     /gene="sufD"
                     /locus_tag="U4A80_000693"
     CDS             32338..33624
                     /gene="sufD"
                     /locus_tag="U4A80_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1974354.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33621..34856
                     /locus_tag="U4A80_000694"
     CDS             33621..34856
                     /locus_tag="U4A80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1974355.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34843..35313
                     /locus_tag="U4A80_000695"
     CDS             34843..35313
                     /locus_tag="U4A80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1974356.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35318..36709
                     /gene="sufB"
                     /locus_tag="U4A80_000696"
     CDS             35318..36709
                     /gene="sufB"
                     /locus_tag="U4A80_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1974357.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        12008 a         6784 c         7868 g        10080 t
ORIGIN      
        1 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
       61 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      121 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      181 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      241 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      301 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      361 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      421 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      481 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      541 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      601 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      661 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      721 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      781 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      841 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      901 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      961 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1021 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1081 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1141 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1201 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1261 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1321 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1381 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1441 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1501 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1561 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1621 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1681 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1741 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1801 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1861 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1921 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1981 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2041 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2101 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2161 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2221 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2281 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2341 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2401 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2461 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2521 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2581 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2641 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2701 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2761 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2821 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2881 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2941 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3001 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3061 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3121 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3181 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3241 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3301 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3361 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3421 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3481 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3541 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3601 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3661 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3721 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3781 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3841 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3901 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3961 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4021 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4081 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4141 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4201 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4261 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4321 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4381 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4441 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4501 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4561 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4621 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4681 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4741 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4801 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4861 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4921 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4981 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5041 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5101 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5161 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5221 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5281 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5341 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5401 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5461 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5521 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5581 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5641 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5701 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5761 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5821 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5881 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5941 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6001 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6061 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6121 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6181 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6241 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6301 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6361 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6421 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6481 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6541 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6601 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6661 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6721 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6781 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6841 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6901 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6961 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7021 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7081 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7141 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7201 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7261 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7321 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7381 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7441 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7501 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7561 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7621 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7681 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7741 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7801 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7861 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7921 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7981 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8041 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8101 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8161 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8221 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8281 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8341 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8401 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8461 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8521 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8581 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8641 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8701 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8761 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8821 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8881 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8941 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9001 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9061 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9121 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9181 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9241 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9301 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9361 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9421 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9481 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9541 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9601 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9661 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9721 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9781 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9841 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9901 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     9961 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
    10021 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10081 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10141 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10201 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10261 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10321 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10381 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10441 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10501 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10561 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10621 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10681 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10741 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10801 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10861 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10921 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    10981 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11041 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11101 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11161 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11221 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11281 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11341 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11401 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11461 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11521 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11581 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11641 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11701 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11761 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11821 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11881 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    11941 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    12001 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12061 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12121 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12181 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12241 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12301 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12361 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12421 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12481 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12541 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12601 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12661 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12721 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12781 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12841 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12901 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    12961 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    13021 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13081 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13141 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13201 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13261 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13321 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13381 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13441 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13501 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13561 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13621 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13681 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13741 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13801 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13861 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13921 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    13981 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    14041 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14101 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14161 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14221 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14281 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14341 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14401 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14461 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14521 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14581 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14641 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14701 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14761 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14821 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14881 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    14941 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    15001 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15061 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15121 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15181 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15241 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15301 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15361 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15421 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15481 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15541 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15601 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15661 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15721 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15781 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15841 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15901 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    15961 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    16021 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16081 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16141 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16201 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16261 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16321 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16381 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16441 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16501 tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa
    16561 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16621 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16681 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16741 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16801 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16861 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16921 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    16981 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    17041 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17101 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17161 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17221 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17281 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17341 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17401 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17461 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17521 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17581 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17641 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17701 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17761 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17821 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17881 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    17941 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    18001 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18061 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18121 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18181 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18241 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18301 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18361 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18421 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18481 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18541 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18601 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18661 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18721 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18781 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18841 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18901 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    18961 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    19021 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19081 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19141 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19201 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19261 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19321 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19381 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19441 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19501 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19561 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19621 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19681 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19741 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19801 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19861 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19921 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    19981 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    20041 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20101 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20161 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20221 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20281 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20341 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20401 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20461 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20521 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20581 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20641 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20701 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20761 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20821 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20881 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    20941 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    21001 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21061 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21121 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21181 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21241 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21301 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21361 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21421 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21481 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21541 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21601 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21661 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21721 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21781 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21841 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21901 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    21961 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    22021 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22081 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22141 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22201 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22261 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22321 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22381 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22441 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22501 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22561 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22621 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22681 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    22741 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    22801 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    22861 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    22921 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    22981 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    23041 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    23101 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    23161 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    23221 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    23281 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    23341 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    23401 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    23461 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    23521 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    23581 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    23641 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    23701 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    23761 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    23821 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    23881 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    23941 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    24001 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    24061 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    24121 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    24181 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    24241 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    24301 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    24361 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    24421 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    24481 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    24541 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    24601 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    24661 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    24721 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    24781 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    24841 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    24901 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    24961 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    25021 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    25081 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    25141 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    25201 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    25261 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    25321 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    25381 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    25441 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    25501 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    25561 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    25621 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    25681 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    25741 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    25801 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    25861 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    25921 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    25981 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    26041 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    26101 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    26161 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    26221 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    26281 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    26341 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    26401 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    26461 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    26521 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    26581 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    26641 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    26701 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    26761 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    26821 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    26881 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    26941 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    27001 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    27061 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    27121 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    27181 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    27241 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    27301 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    27361 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    27421 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    27481 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    27541 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    27601 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    27661 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    27721 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    27781 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    27841 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    27901 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    27961 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    28021 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    28081 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    28141 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    28201 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    28261 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    28321 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    28381 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    28441 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    28501 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    28561 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    28621 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    28681 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    28741 aaaaattata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    28801 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    28861 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    28921 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    28981 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    29041 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    29101 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    29161 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    29221 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    29281 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    29341 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    29401 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    29461 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    29521 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    29581 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    29641 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    29701 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    29761 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    29821 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    29881 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    29941 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    30001 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    30061 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    30121 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    30181 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    30241 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    30301 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    30361 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    30421 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    30481 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    30541 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    30601 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    30661 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    30721 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    30781 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    30841 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    30901 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    30961 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    31021 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    31081 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    31141 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    31201 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    31261 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    31321 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    31381 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    31441 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    31501 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    31561 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    31621 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    31681 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    31741 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    31801 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    31861 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    31921 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    31981 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    32041 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    32101 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    32161 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    32221 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    32281 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    32341 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    32401 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    32461 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    32521 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    32581 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    32641 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    32701 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    32761 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    32821 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    32881 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    32941 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    33001 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    33061 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    33121 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    33181 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    33241 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    33301 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    33361 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    33421 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    33481 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    33541 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    33601 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    33661 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    33721 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    33781 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    33841 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    33901 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    33961 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    34021 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    34081 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    34141 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    34201 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    34261 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    34321 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    34381 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    34441 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    34501 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    34561 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    34621 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    34681 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    34741 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    34801 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    34861 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    34921 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    34981 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    35041 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    35101 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    35161 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    35221 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    35281 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    35341 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    35401 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    35461 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    35521 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    35581 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    35641 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    35701 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    35761 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    35821 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    35881 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    35941 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    36001 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    36061 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    36121 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    36181 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    36241 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    36301 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    36361 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    36421 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    36481 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    36541 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    36601 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    36661 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    36721 gagctagaac ccttgatata
//
LOCUS       DASJRQ010000015        36524 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJRQ010000015 DASJRQ010000000
VERSION     DASJRQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.015"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(387..1853)
                     /locus_tag="U4A80_000697"
     CDS             complement(387..1853)
                     /locus_tag="U4A80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1974358.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="U4A80_000698"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="U4A80_000698"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1974359.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4753..5112)
                     /locus_tag="U4A80_000699"
     CDS             complement(4753..5112)
                     /locus_tag="U4A80_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1974360.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5142..5483)
                     /locus_tag="U4A80_000700"
     CDS             complement(5142..5483)
                     /locus_tag="U4A80_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974361.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5480..6154)
                     /locus_tag="U4A80_000701"
     CDS             complement(5480..6154)
                     /locus_tag="U4A80_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1974362.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="U4A80_000702"
     CDS             complement(7330..8628)
                     /gene="asnS"
                     /locus_tag="U4A80_000702"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1974363.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8668..9858)
                     /locus_tag="U4A80_000703"
     CDS             complement(8668..9858)
                     /locus_tag="U4A80_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1974364.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9879..10406)
                     /locus_tag="U4A80_000704"
     CDS             complement(9879..10406)
                     /locus_tag="U4A80_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1974365.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10453..13242)
                     /locus_tag="U4A80_000705"
     CDS             complement(10453..13242)
                     /locus_tag="U4A80_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1974366.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13389..13589)
                     /locus_tag="U4A80_000706"
     CDS             complement(13389..13589)
                     /locus_tag="U4A80_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1974367.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14042..14362)
                     /locus_tag="U4A80_000707"
     CDS             complement(14042..14362)
                     /locus_tag="U4A80_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974368.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14865..15983)
                     /locus_tag="U4A80_000708"
     CDS             complement(14865..15983)
                     /locus_tag="U4A80_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974369.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16454..17761
                     /locus_tag="U4A80_000709"
     CDS             16454..17761
                     /locus_tag="U4A80_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1974370.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17882..19081
                     /locus_tag="U4A80_000710"
     CDS             17882..19081
                     /locus_tag="U4A80_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1974371.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19108..20376)
                     /locus_tag="U4A80_000711"
     CDS             complement(19108..20376)
                     /locus_tag="U4A80_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1974372.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(20546..21187)
                     /locus_tag="U4A80_000712"
     CDS             complement(20546..21187)
                     /locus_tag="U4A80_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN1974373.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(21596..22099)
                     /locus_tag="U4A80_000713"
     CDS             complement(21596..22099)
                     /locus_tag="U4A80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1974374.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(22222..22986)
                     /locus_tag="U4A80_000714"
     CDS             complement(22222..22986)
                     /locus_tag="U4A80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1974375.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            23094..23369
                     /locus_tag="U4A80_000715"
     CDS             23094..23369
                     /locus_tag="U4A80_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1974376.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            23929..24879
                     /gene="yidC"
                     /locus_tag="U4A80_000716"
     CDS             23929..24879
                     /gene="yidC"
                     /locus_tag="U4A80_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1974377.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(24925..25326)
                     /locus_tag="U4A80_000717"
     CDS             complement(24925..25326)
                     /locus_tag="U4A80_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1974378.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(25340..26149)
                     /locus_tag="U4A80_000718"
     CDS             complement(25340..26149)
                     /locus_tag="U4A80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1974379.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(26136..27026)
                     /locus_tag="U4A80_000719"
     CDS             complement(26136..27026)
                     /locus_tag="U4A80_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1974380.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(27039..27518)
                     /locus_tag="U4A80_000720"
                     /pseudo
     CDS             complement(27039..27518)
                     /locus_tag="U4A80_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(27694..28635)
                     /locus_tag="U4A80_000721"
     CDS             complement(27694..28635)
                     /locus_tag="U4A80_000721"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN1974381.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(28632..29627)
                     /gene="lacD"
                     /locus_tag="U4A80_000722"
     CDS             complement(28632..29627)
                     /gene="lacD"
                     /locus_tag="U4A80_000722"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1974382.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(29620..30816)
                     /locus_tag="U4A80_000723"
     CDS             complement(29620..30816)
                     /locus_tag="U4A80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1974383.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(30834..31562)
                     /locus_tag="U4A80_000724"
     CDS             complement(30834..31562)
                     /locus_tag="U4A80_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1974384.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(32003..32977)
                     /gene="lacD"
                     /locus_tag="U4A80_000725"
     CDS             complement(32003..32977)
                     /gene="lacD"
                     /locus_tag="U4A80_000725"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1974385.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(32974..33432)
                     /locus_tag="U4A80_000726"
     CDS             complement(32974..33432)
                     /locus_tag="U4A80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1974386.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(33429..34892)
                     /locus_tag="U4A80_000727"
     CDS             complement(33429..34892)
                     /locus_tag="U4A80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1974387.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(34874..35806)
                     /gene="pfkB"
                     /locus_tag="U4A80_000728"
     CDS             complement(34874..35806)
                     /gene="pfkB"
                     /locus_tag="U4A80_000728"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1974388.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35948..>36524
                     /locus_tag="U4A80_000729"
     CDS             35948..>36524
                     /locus_tag="U4A80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1974389.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT        10692 a         7848 c         6210 g        11774 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacatgact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatttct tccatacaga tgttttggct tattctttct
     4381 ttttctcttc gatcattgcc cctgctctta tccaacccgc acttgccttc ttgatcttga
     4441 caggatagca actaagtttg aagcctgttg ctggcaattt gtctaaattc attaattttt
     4501 cgataggaca atatcttttt tcaatccctg caaaatgtcc ttcccagata ttcgaagctc
     4561 cactacgctt tcaaaaatat agaaagagtt ctttaatgaa aattatttca actttccata
     4621 aacagtaatc ccatataaat aaaagacgta aaagtcacat gggagaactt atacaggaaa
     4681 acacatagag tcaggtgaaa tacatgtatc ccacctgact ctatgcgttt tctaaccttt
     4741 attattctct ttttatacaa tttctgcacc gaatggcatc aacgccaatc gagcaagttt
     4801 gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga tgccgctgac
     4861 gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat taacgatcaa
     4921 tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc
     4981 gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc aaagtatccc
     5041 cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga tgacatttcc
     5101 taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg ggcaaaaatt
     5161 gattttattc ttcttttttt agcttccagc atcaagtatt ctgcaaattt tttttggatt
     5221 tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga tggtcgctga
     5281 tccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct
     5341 tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc cgtttttgct
     5401 tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtaa atcttctttt
     5461 actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta tctccactaa
     5521 aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca tgtccgccaa
     5581 tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca atccctaaag
     5641 cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca agtttacgaa
     5701 caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg gttataggaa
     5761 gtacaaacgg ctccatcaag atgatgttgc agatttccgc tgtttgaagt tcatttagta
     5821 attttcgata aacggattca aattgctgcg cagatttctc agttccgaat atagcctgat
     5881 cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag tcaggttgta
     5941 aagctaaaca atcttcttgc cagcgtactt gcagatctgc aattttgtct cctcctatcc
     6001 cacggttcat gcattcaaat ttcatcgttg gatattggga gattagtttt ccggcaatca
     6061 aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct gcgtctgtaa
     6121 tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt tttgaataaa
     6181 acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag agaaaatata
     6241 tgtataaaaa atgacaccag cctagtccct tcaatcaatt gaatgaacgc tcatcaagtg
     6301 tctagttttt tatacttttt ttcttaacgt gccggattcc ccttgattcc ttcgttgttt
     6361 ggtccagaga aatcgatcac acctaagctt ccgtcaatca gaccactgat gacgccatca
     6421 gaacatatat ttataggctc atctaagact tccagtaaat gttcttttac taatatctct
     6481 tctagattgc ttgggataga ttggtcataa ctttatctgg tccgatgatt tcaaggatga
     6541 attaaactta catatggatc attgcatgct gaaaatacgg acctactcca tcttcgaatg
     6601 ttactattag tttgttttca ggtactagat gctgctttat ttcaacagca gaagcatcat
     6661 caaatcagat ttccatttga ataaccttct ttgtatatgt ttgattgatt gcgatttcgt
     6721 tatagaacct tgtgaatcaa atagcaattt cttttgcttt cttctcttgt ctgctggaaa
     6781 cttcctttat cccgtatttt aaaaaaagaa ctgcgagagt tcgcagttct tttttttaaa
     6841 taaacgttgc tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa
     6901 aactgttttt tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt
     6961 gatataaacg gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg
     7021 agaagctatt tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc
     7081 tcttccttaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     7141 tgtggaaagc catttccaca aaatttttct taatactcga aactaaacag ctttttcaca
     7201 acctctccac taaacggtat tcaaaagctt acatctgcgg taataagtcc cgccaattca
     7261 aaaacatgaa cagctgtttt tgcttggctg ttctaattac ttgatgcaga acagcttttt
     7321 catcacctct tatggataaa ttcggttcaa caaacgtggg aatggaatag cttcacgtac
     7381 atggtcgata ccagcaatcc aagtgactgt tcgttcaagg cctagaccaa aaccagagtg
     7441 cggaacagaa ccgtatttac gtagatcaag ataccatgaa tattcttttt catccaaacc
     7501 gtgattacgg atttgttcta gcaagaaatc atagtctgtt gcccgttcgc ttccgccaat
     7561 aatttcaccg taaccttctg gtgcgatcat atctgcacaa attaccacgt cttctcttgt
     7621 tggatgtggt ttcatgtaga atggcttgat ggcttttgga tagtttagga taaagacagg
     7681 ctgttcgaat gaattcgcaa tgaacgtttc atgaggtgaa ccaaagtctt cgccccattc
     7741 gatgtcatca aatccatttt tcttcagtaa ttcgattgca tcatcgtaag taatacgagg
     7801 gaatggcaat tttgtataag tttttaagac ttctttgtct cgtcctagta catctaatgc
     7861 atattcgcaa tgatctaaaa cattttgtac taagaatgct acatattgtt cctgtacttc
     7921 taaactttct tcttgatgag tgaaagccat ttctggttcg atcatccaga attcgatcaa
     7981 atgacgtcgt gtttttgatt tttcagcacg gaaagtcgga ccgaatgaaa agacttttcc
     8041 gaaagccata gcagcagctt ccatgtataa ttggccactt tgagataaat aagctttttg
     8101 atcaaagtaa tttgtttcga atagatccgt agtgccttct ggtgcagaac cagtcaagat
     8161 cggcggatcg attttgacaa aatgatgttg attaaagaat tcatacgttg cacgaatgat
     8221 ttcatttctg atctgcataa tcgcatgttg tcttgaagaa cgcagccata aatgacgatg
     8281 atccatcaag aaatctgttc cgtgttcttt tggtgtgatc ggataatcgt ggctctcgct
     8341 taccacttcc acatttgtaa ttccgatttc ataaccaaat tttgaacgac tgtcttcacg
     8401 gatttctcca gttactaaca cggatgtttc ttggtgtaga tttttagctg tttggaaaac
     8461 ttcttccgac acttcgcttt ttacaactac accttggaaa aaggctgttc cgtctcttaa
     8521 ttgtaaaaat gcgatttttc cgcttgatcg cttatttgcg acccaagcgc cgattgttac
     8581 tgtttctccc acatggtttt tcgaatcgat gatttgaatt tgttccacgt atgactctcc
     8641 ttttatattc cttgaatttc ttcaggatta attttgactt ttactctcaa taaattgctt
     8701 gattctgcgt accgcttctt ctaacgtagc gcgatcagtg gcataactca tgcggacatt
     8761 ttctggtgcc ccaaagcctt cgccagtcac aagcgctacc cctgtttctt ctaaaagatc
     8821 ttctacccat tgtgtgacat tttcatactt acacatgcgt agcgtttctt ttacattagg
     8881 aaaaagatag aaagctcctt gcggcttttc aagagaaaca cccggcagtt ctgccacaag
     8941 cggatataac gtattcaggc gttcttcaaa ggcgttccgc atttcttcta ctgtatcttg
     9001 ctcacctttc aatgcttcaa cagctgcata ttgactgact gctgtcggat tgctagttga
     9061 ttgggaagca acagcgatca ttccattgat gatcacagga tttcccaccg cataaccaat
     9121 gcgccagcct gtcatcgcat atgttttaga cacgccgttg atgatgatgg tctgattttt
     9181 gattgcttct gaaatcgttg caattggagt aaactcattg ccattataaa ctaaacggcc
     9241 ataaatatca tcagcaacta tcaaaatatc attttttact gcccattcac cgattgcctg
     9301 cagttcattc ttgctataaa tcattccagt cggattcgat ggagaattga tgattacggc
     9361 ttttgttttt tctgaacgtg cttgttctag ctgttcaaca gtaactttcc agctattgga
     9421 ttcttcccct ttcacaaaga caggaagtcc ttctgccagt tttacttgct caccgtaact
     9481 gacccagtaa ggcacaggaa tgatgacttc atcttgcggg tcaaggatcg tttggaaaag
     9541 ggtatacaaa gcaaatttcg ctccgtctgt tacgactgtt tgtgaaggtt catagacaag
     9601 tccatagttt ttcttcagat aatcaacgat tgcctgtcga agctctggga ttcctgctgt
     9661 tggtgtataa tagctagctt tcccattgcg gattgcttcg attgctgctt cttggatgtt
     9721 ttctggtgtc gcaaaatccg gttctcccac agtcaaactc agcacatctc tgccttttgc
     9781 ttttaatgcc ttggcctttg ccgcagctgc caaagtgaca gacggttcta atcgttttgt
     9841 tctttttgat aactccatcc cattcattcc tttacctttt aaattccctt gattgtcttg
     9901 acttcatctc cagattcaaa gccaatcagg taataattca gtttcccatt tgtttctttt
     9961 cctactactt cccacacagg ctgatcatca tacatcccta aagcagcttt ttcgatttgg
    10021 atttcaggat gggccgcagc aattgtctgt tttgcttcgt tttcagtcag tccctttgtt
    10081 tgatccaata cttttacttt ttcaccagat ttaggaatga taacggcgat ttcttttcct
    10141 ttatcatttg tacctgtcag tgaaaagtat gtcttctttc gagtaaacca ataaaaatga
    10201 tcggtttcct caagatttgc atatttcttt gcgatttcta ctgcttcttt cttcgcctgc
    10261 accgccggac gactggaacg caagtaaaaa atcgagctga tcacgatgac cgctaataaa
    10321 aatacgatca agccaagtaa cacgcgatta atcgtttttt cctttgtctg ttttttcttc
    10381 aagggtcgtc gctcctttat cttcattcct tttcattata gcagatttta attatgaaaa
    10441 aagacagctc ctctaacttc cttcttcatt tttctttaag aattcatgga tatcctgata
    10501 gatttcctcc atagctagct ctttgatcgg tacttttttc ggtaatgcat ggatgatccg
    10561 tgcgccatat tttgcagtga ctagtcttcg atccaaaata agcaatacgc cacgatctgt
    10621 ctccgtcctt atcaagcgac ctaatgcttg gcgtaacttc aaagctgctt taggtacgga
    10681 ttcctggtaa aaaggattga tcccctctga ttctaaataa gcgttcctag ctttgaccat
    10741 cgggcgaagc ggattatcga agggtaaacg tgtgactaca acgatctgca aagcatctcc
    10801 aggcaaatct acaccttccc aaaagctgtc agcgccaaac aatacgcttt tttctgacaa
    10861 aatgaagcgt ttcaaaagct tctcccggct tccaccaaac ccttgagcta gtatttctcg
    10921 tcctttttct agcatagaca gatgcatttt ctgatagacc ttctgcaaaa catcatgaga
    10981 agtgaataac acaagtattg gccgatcttc ttgttcgatc agacctgtaa gtgctgtctc
    11041 taaataatcg atatatgctt gattgctgac ttgatggacg gcaggtgcct cttttggcac
    11101 atagatcctt gcctgtttct cataatcata aggagaggca atcactttaa gaggtgctgc
    11161 ttcaatgcca agcctcttag agagatagtg tcgattaggg ccgatcttca gcgttcctcc
    11221 aatataaaga atacgatcat atcgaggata ccaaacagtc gacggcaaca tagccgcttg
    11281 aaaatcatgg agatgaaaaa tcatctgctg cgctgtgttc ccaggaacga accaatggat
    11341 caaatgatct cgccagtctt ccagccattg atccatcagc aagtattgct tgtctaattg
    11401 atcaaatagg ctatataaac ttgctaaatc gatccgcatc tggtttgtcc agctttcttg
    11461 gataatctca aaagtcagac gcatttgtga ctgtagctgc tggatctcct gataaaaaag
    11521 ctgcaggtga tcaatggctg tcccagattc cagtggcagc tgatccaaca tctcttttgt
    11581 catcatgact tcttctggat actgtccgtt aggttgcttg ccgctcacta aactcattaa
    11641 tccttctgcc atacaaatct gtgcttcgca tagcgcagaa agttcttctc gataaatcga
    11701 taaccagcgt aaggcttcat gttctttcgg taaatagctt tgtattcggt caaacagctg
    11761 atttggttct tctaattgct ggatttgttt atggaaatag ttaatactta atcgtttgtc
    11821 atttactttt tcagcgattt ccggcaaatg atgtacttca tcgatcaaca aaaatggact
    11881 agctggaagc aatggttctt ttcgctgtgt ttcttgtact aaaaacgcat gattgacaat
    11941 caaaaaatta ctttgcctga ttttcttttg caagaaacga acaaagtctt gttcgtaaaa
    12001 tgactgattt cttgctaaaa atccaatacc tctgtgcgcg atatctgcaa agaatgaatg
    12061 gttcaaattt gttaaatgta attcatccaa atcaccagtc ttcgtttgcg tcagccaaac
    12121 taagaccgcc atttgataca aagcatattg tttttgttct ggcggttcca caagtgtgcc
    12181 tttaaaacgc tgcaaatcaa tatagtggcg atagcttttg ataattacag gttgtatggg
    12241 ctgatctagc aattgattca gcaatggaat gtctttttct atgatctgct gctgcaaaac
    12301 tagcgaaacg gtactgatga cagcgggttt ttcaggtgta gccaaataac tcaatggcag
    12361 cagataaccg attgttttac ccattcctgt agctgcttcg atcatcaaat cttttgattc
    12421 atcttttgtg aaatggtcgt agaccgcatt catcagtctg ttctgttctt ttcgatacat
    12481 cagtgtttta ccaaacattt tttcttttgc ttgttttttt cttggatatg tccgctcgcc
    12541 ataatggacg gatgtgaata gttccacttc ttttttctgc aaagctagcc catcgacgat
    12601 ttctaatgag ggatcaagtg gctctggttt ttctttcatt tcttcaatca catgataaat
    12661 gaaacgactc gtatccatac ctgtgtgtct acttaatcgc gcaatctgct ctaacgtgat
    12721 gattggcaat tttctcatcc gttcttcgat cagcaaaaga agttgacctg tcacttcggc
    12781 atcactatct gcctgatgag gattctcatg aataaatcca aggctttctg acaaatcagc
    12841 taagcgaaag cttggttccg tcggcaagaa aatttgtgca agttctactg tatcgatgcc
    12901 tggtatcttc aaactaggaa gtccgcatcg catgagttca tggttcaaaa aattataatc
    12961 aaaataaata ttatgcgcga caaatatggt gtctgccaaa agattgtaaa tcgtttgggc
    13021 gacatcttcg aaataaggcg ctttttggac gcgttgattc gtgatccctg ttaaatgctg
    13081 gatttgtttt gaaatcttcc gccctggatg aatatcgatc gaaaaacgtg aagtgatccg
    13141 acctgattca atcatgacgc agccaaattg gatgatccga tcttcttttg gattcgtccc
    13201 agtcgtttcg atatcaacga ctgcataagt tcttggtttc aaaatatctt cctcttctcc
    13261 tgcctctaat gttcttttga aattatacta caaagtgcag aaaaatactc tcatcgcttt
    13321 ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc tgggcgttta ttttttagta
    13381 taggttaatt aagctttagt aacgtttgtt gcttgaggtc cgcgttggcc ttcttcaaca
    13441 tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc cgtcgccttg gatagctgag
    13501 aaatgaacga atacatcgtt tccgttttct gcagtgatga atccaaatcc tttttctgag
    13561 ttaaaccatt ttactgtacc tgtttccata ataaaaatcc tccttgtgta tcaacacata
    13621 ttttgcaatt acttgaatgg tgtatatctg gaagatgttt atttgaaaca agctaccaca
    13681 gattaccgaa caaaaactac tcctatagta tatcataccg tttacaaaaa agctagagtt
    13741 tttcgacttt ttttattaaa aaaagttttc tcagcgatta cataaagtga aaaaccgaac
    13801 ttttttttaa aataaacgac atattgttca gttggtgttt ttttgctctg atgttctttt
    13861 ttaaactaaa aaaattattc tttctgacta cttatcggtt ctgtttcaac ggtcgaggct
    13921 ggggaagatt ctttagtagg tctgatgttc tcatggtatt tctgatagcc tatgacagtt
    13981 accagaagaa caaggcaaac caatacgacc aacgagatta gtttttcttt tttgctcata
    14041 tctatacctc ataaataaca ggatgatgtc catcatattt ctgagtatga gtaaaagcaa
    14101 aagctgcagc tgtagaatgt aagaaatgcc agcaactttc ataatcacag gtcggacagt
    14161 accaaaagta accaaatctt ggctgcaaag accgttcttc tgtttcagga atcgcagaat
    14221 caagatctgg tgtctgtaca taaacatttt tgtacagacg tgtgcctgta ataggaaacc
    14281 gctgtgtttc ttcagcaaaa actggctgac ttgttaaact tagggataga gacagcaaac
    14341 accataacag tctttttttc ataaagacct ccttatcgat aagtttttcc caattaaaat
    14401 catatctcaa gaacgcccgt tattcacatt ttgttcataa atgatgtttt ttgtatcata
    14461 atcgtcaaaa agaggtcgtg accacgtcac aacctctttt ttaaacggtg ttccagaagt
    14521 agcttcttcg gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct
    14581 cttcttattt cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa
    14641 agcttgcatc tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg
    14701 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat
    14761 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gtgaaactat ttttccgttt
    14821 gtttctctaa tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc
    14881 gctgtcagac tcttttcatc tggcgcatct ttttgatcca aaataaacac ttttccgcca
    14941 ttaatgatca catcatctgc taatgcattc aacacttgac gccgatcata ttctgtttca
    15001 ggatcttcac cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt
    15061 ccttctttcg cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt
    15121 ttattgtatt cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct
    15181 tcgatatctc taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa
    15241 tagggatttt tagcaatttt tttgaacatc gtctgatttt ctggtagagc aaataaataa
    15301 atcggccatt gttctggatt ttcgaaagaa tccttcaagt acgtatcaac tgcttggtaa
    15361 tagttgaccc aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact
    15421 ccatctcggg ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca
    15481 gatcccaatg cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact
    15541 tgtttgttgc tgacttcata caatttcatg gagtcgcggt tcaatcccaa taaataataa
    15601 gaatagttga actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag
    15661 tattgatcgt ctaccgggat actcaaacga tggatatagg tttggtcagc agttataatg
    15721 accgctacac tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt
    15781 ttttcttgga acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct
    15841 tctttcgcaa aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc
    15901 gtattcagca tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgacaat
    15961 aattctttat ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc
    16021 tatctaaagt ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa
    16081 acggtttcta cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat
    16141 tatttagccc cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata
    16201 aaagaggtgg atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta
    16261 tttgctttca gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta
    16321 cgtaaaaacg accgctaacc ttccataacg tgcgttctta tcctaaagtt ttttcagaaa
    16381 cattgaaact ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac
    16441 aaggagatcg gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat
    16501 tctttgcctt gcttcttttc taccagctgt tgctgtgcaa gctgaagata ctttcaaagt
    16561 aaatgcaaaa gcagcctttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga
    16621 tggagaaaaa accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct
    16681 tgatcaagta aaagaaggga aactatcatg ggatgataaa gtttcaattt cagattatgc
    16741 agaaaatttg agtgtcaccc cagatttatc aaatgttcct ttgcataaag aaaacaccta
    16801 caccgttaaa gaattattcg actctgcaat catccagtca gccaacgctt caatggttgc
    16861 cttggcagaa aaaatctctg gcagcgaagc aaaattcact gaacgaatga aagaacaatt
    16921 gaaagattgg ggtatcaaag atgcaacgat cgttaatgca tcaggactga ataactctta
    16981 tcttggcgaa aaccgaccag aaggtactgg agaaaacgac gaaaatcaaa tgtctgctca
    17041 agatgtggct atcgttgccc gtcatttgat cttggatttt ccagaaatac tggatgtatc
    17101 tagcactacg acacaaatgt ttggcgaaaa cacgcaatca ccagtagaaa tggtcaactg
    17161 gaactggatg ctcccaggat tcgtgaatta taaagaaggc gtggacggat taaaaactgg
    17221 tacgactgaa cttgcaggag cctgtttcgt cggtacgatc actaaaaacg atcaacgaat
    17281 catcactgtc gtattgaatg cagacggcca tgcggaaaac ccaagtgccc gtttcaatga
    17341 aacagctcgc ttaatggatt attgctacga caattggtca gaaaaagagc ttggaaaagc
    17401 caatgcaagt atccctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt
    17461 gcccgttgtg cttaaaagtc ctgtaaaagt atgggtacgg aacgatatgg atactggaaa
    17521 attaacaatc acaccaactc ttgacaaaaa acaagtcact gacaatgaga tccaagcacc
    17581 tatggataaa ggaacaaaaa tcggtaccgc aacgatcacc ttggcagaag ataaattagg
    17641 ttatcttgaa gacaacaacg cgccatccac agaaatcatc acggataaaa ccgtagaaaa
    17701 agcaaatatt ttcgttattg gctggcgacg cgtcgcagaa tttttcggta acttgtttta
    17761 attgaacaaa aagaaaaaag catctttcca tgtggaagat gtttttttgc tttttctagt
    17821 ttaaataaca gcttttttca tctattttcg ttatactgta taagaaaaag gagttgaatt
    17881 tatgaatcta accagagatc tgattttttt ggttctctat cttattgggg tattctccat
    17941 cgtttattgg cgaaaacaga aaaaggatcc ttccttgttc ttttacagca gtacatggac
    18001 gtttgcggta atcttgactt attttgtcgt gttggaaatt ttttattcga ccttttattt
    18061 tttgattccc ttagtgtttt tcagtatctt tgccttttcc tatttttcag aaaagcgaag
    18121 gctgttgaat ggtcttttat tcaatatttt cctcattagt tttggtatct acttgtttgt
    18181 cttgctatac gaaacacaaa atatttttct tggcggactg attgccttga tcacgatccc
    18241 gttactgctt gtctttctat tcggcattta tggattgatc gtttttcttt tttggaacgg
    18301 tgtcacagtt ttacgtagag agtcgcattc attagctaat cttttaacgt tgatactagc
    18361 catttttctt acattgtttc ttgtttttga ctttttcttg ttaaaatatt taccacaatg
    18421 gataaatgct ttgtttttct gcatcccatt gatcttgatc tatctattta tcgtattcta
    18481 caactttttg actgtctcat tcttgtatca gttcaaccgc cctcgataca accaagactt
    18541 catcgttgtg ttaggcgccg ggttgatcaa tggagaaaca gtttctcctc ttcttgccaa
    18601 gcgaatcaat aaggcgattg ctttttattg ggcacaaagc cgtgcgacat taaatccgcc
    18661 tatcctatta atgtcgggtg gacagggagc agacgaaaaa gtaccagaag caatggcgat
    18721 gaaacaatat gccatggaac aaggaattcc tgaaagagat attcttgtcg aaaccaattc
    18781 gacaaccact ttggagaata tgcgctattc aaaggaaatc atggatcagc aaatgaaagg
    18841 tccctatcgc gcgatcttca gttccaacaa ttaccacatt ttcagagctg gtctctatgc
    18901 acgacaagcg aaactgaagg caaatggcat tggggcaaaa acagctttct attacttgcc
    18961 taacgcgttt ttaagagaat acatcgccat cttgatgatg aacaaaaaaa gacatatgat
    19021 catttgcgga ttgcttttcg caggttccgc attcttatca ctgatcactt tacttttcta
    19081 aaaaaacaag gtgtgacaaa acatatgtca caccttgttt ttttctttta ttctcttgac
    19141 gattggcaag aatagtattc cttttttgac ggctacttgc tgtgcagaat aaataccgct
    19201 gtttcctgaa cacataaagc tgatcaagca gatcataaag aagtaagcag cagcttgtga
    19261 accaaataat tcgattccca tcacaaaaca ggcaatcggt gtatttgtcg caccagaaaa
    19321 taccccgata aaacctaatc cagcaagaaa aggaacagaa acatggagca aaggcgctag
    19381 tactgcgcca agcgttgcac caatctcaaa tagtggtgtc acttctcccc cttggtaccc
    19441 cgcacctaaa gacaagaccg tgaagaataa tttcccaata aaatcataag catgtgcctg
    19501 tccattaaat gattcctgca gtaacggaag actcaaccca aggtaacgtt gtgtttgcaa
    19561 aatcaacgct gcggctacaa ctactgctcc acctatcgta tttcgccaaa caggatgagg
    19621 aatccattgc gcataccgcg cttttaaaaa gacgatcgaa cggctgaata cccaaccaat
    19681 caaaccaaaa gcaatcccag caacaaaaac tttgaagaac agcaacatac tccattcagg
    19741 aatcacaccc atttggtaat gtagatgaga aacaccatag ctttctgtca tgaaatttgc
    19801 aaaaagcgca gcaaagaagc taggaaatat tgcttctgtt cggactttac cgattgccaa
    19861 cacttctaat ccaaataatg ttccagctaa aggtgtgcca aatacagaac taaaacccgc
    19921 actgattcca ctgataacca aaatctcccg ttctgcttta tccaatcgga ataaatgcgc
    19981 tatattatca gctaatgctc cccccatttg aactgctgtt ccttcacgac caacagatcc
    20041 cccaaataaa tgggtagtga tggtcccaaa caaggtcaat ggaatgagcc gcaaagggat
    20101 cttctcttct cctccattcc cttgatcgat cactaagtta ttcccacgac tagaaagacc
    20161 gccatgatac gcataaagat aagcaaaaag cgcgccgctg actggcaata gataaagtag
    20221 ccaaggatgt gctagtcgga tatctgtcac gaacgtcagg ctatttaaga aaaaagcaga
    20281 caagctcccc atgaatactc cgagaatcgc tgtgaccacc agccacttca acatgtacat
    20341 caaaatacgc actggactcg gtaattgttt ttccataaaa aattcctcct caaataaaaa
    20401 aacgcctact aaagaaaggg gctggcccca gactttagta ggcgtcatta gctatcttac
    20461 gttagcattt aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt
    20521 tctgtaaaaa acaataaaat tatcgttatt tctttacacg ttcagcaaat tcatagaaag
    20581 gagccaattt cgttaactgt tgattacatt gatcactatc atgacaaata taattcccct
    20641 ttttcgtata cgtgccatca gaaccggctt tcgttgtaga aagaaagagc gagacattcg
    20701 atgttttctg acagatcgca cagattccct tattaatcgt tggtgataaa gtaccgctga
    20761 tcccttttaa ttttccatct tcgtaataaa ccatgaattt tttttgcgtc cctggatcat
    20821 tccaaccgat gtacgtatgt gcttgcaagt cgatttcatt ccaagcgggt tgtttgagtt
    20881 ttttcacttt tcggaataat ttctcgattt gtggtttgct cggctgcata aacggctgga
    20941 cgatttgctt cagctcctcc aatgctcgtt ctgcttgcgt ttttgtcagt tttacatcta
    21001 aaactgtcgt taaaaacgta tcgatttctg atggttcgta acctaataca tcgcgaatct
    21061 tttcagctgc tagagcctga accgtttgta ccgtttttga gtcattgaca gatgagtacg
    21121 catttaataa ctgctctact tctcttttta taaaaaaata ttgatatggt ttcattgttt
    21181 gttccatgat tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga
    21241 tcttctgcaa agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg
    21301 catcttatca tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat
    21361 aaaagtaaac cgtatcttga gcagatagtc aactgcaaac agaaaaagga ctgtgacaaa
    21421 agtcgtgtca aagtcccttt ctacaaacaa acggtgttcc agaagtagct tcttcaaaaa
    21481 ataagccgga atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
    21541 gaagctgacc cacttctgtc acaacctcaa tatggtctat tattggtttt acctgttaaa
    21601 tcgaggattc ttttccgaag taacgttgcc atctcttacg catacttgct gacataggct
    21661 gagcggcttc tttgactgcg cagtatttgt ctacgaatgt tacgatatat ccttctttgt
    21721 acttaggcgg tgcaatcgtt gcgccccaca tgtgcttcaa aatgatatct cgctcaagat
    21781 ccgacaaatc ggtgatcttt tctgcattct ttacagcgat tcttggatgg atatacgcat
    21841 gtgtgccttc atcaaatttc gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc
    21901 cagcacgcgc tgttgcttta gcattgccac cccatttttt tgcgagcaga taactatagt
    21961 aagaaacact gatggaatgc tccaaacgag tggaatgaac atgctgtgtg taattcgcta
    22021 attttttcac tgcttctgtt tctaaaagat cttctacata agacatatat tcttcgtctt
    22081 cacgccattt ttctgtcata attatcgatc tgccaccttt ttgaatttca ttttcttctg
    22141 aaattgatct aagtatacca ccagtgttcg gattattcta gtcgccttgg ataaaatagc
    22201 caaagcttaa tagttcctta atcataaaga aaaatgataa agaacaatcc ctgccgcaac
    22261 cccaacattc aacgattccg catttccttt catcgggata tatacgttct gatcagtcaa
    22321 agacaagatt tcctgactta ccccctgacc ttcgttcccc atgattattg cgaagttatc
    22381 tgatttttct actttgtcta aggcgtgtgc ttcttcattt aattctgtgc catacactgt
    22441 aaaccctttg tctttcatta aaggaatcac ttctgtcaaa tctttttgca acacaggcaa
    22501 atgataattg cttccctgca tgctgcgcag cacctttgta ctataaatat ctgccgttcc
    22561 tttgcctaat aaaacaccag ccatatcaaa tgcatctgcc gtacggatca tcgtccctac
    22621 atttccagga tcttgtacat tatctaataa cagccatcct cctgaaaaat ctggcgtgcc
    22681 ttcagccgtt aacgccatca ctgctaagat tccttgtggt gtaggtagat ccgatacagc
    22741 atccatgacc tcatccgaaa ctagaaaagt agaaatctct ttgtttgcgg ccagccagtc
    22801 tccccattct ttcatgcctc gctgtgtcac aaaaagttct tttatttctg catcagacga
    22861 cgcagcttct tcaatcagat ggaacccttc caaaagatat tcttcctttt cttcccgata
    22921 tttcttttta tgtagttttt ttaattcttt gattcttgga ttttttgctg attggatctc
    22981 tttcacattc ctcacctccc catgattata tcatggaata ggtgcttgag cttgtttcat
    23041 cctaaaaaaa tcggtatcat taatttataa aataggaaag aaaggtgacg aacatgcgca
    23101 aagttaaaat gaacgtccaa ggacgcgtcc aaggcgtagg atttcgctac atgaccaaga
    23161 tggtcgctga tcaactaggc gtgactggaa cggtaaaaaa cgaagatgac ggctcagtca
    23221 cgatcgaagc aatcggcaat gacgacatca tccagaaatt tattgaagaa gtcaagaaat
    23281 ctccgagccc aagtggacga gtgcagtatg tggatgtcca agaagacccg atgattgagg
    23341 aacgaaagaa atttgatgtg gtgggataag gttgtgaaaa agctggtctg catcaagcat
    23401 taaggacagg ttgtgaaaaa tagctctcca tatttttcaa caggctggac ttaatgccgt
    23461 agatgcaagc ttttgaacac cgtcaataag gttgtgagaa agtcgcttag ttttgagtag
    23521 taaggagaat tttgtgaaaa tggctttcca catttttgca taaaatttga cttaataccg
    23581 agaaactgac ttttgaacac cgtcgataag gttgtttttg agacgctttg cctcaagtat
    23641 taaagatagg tagtaaaaaa gctgatctat aacttgcaat aaatttgctt aacccatatt
    23701 ttccagacag aacaaaaaag tttttagttt tttaaataag aaagaaacac ttggaatcac
    23761 tcttttcttt ccaagtattt ctttcttgat tttttgtctt tttttcaaaa tttttcgcta
    23821 cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc catgcgcttt
    23881 tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat gaataaatgg
    23941 aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc tggctgtgtg
    24001 cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt actcgtccat
    24061 cctatgggcc aagcgattac atatctagtt gagaatttca attggtctta tggctgggca
    24121 gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat tagtcaatcg
    24181 aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt tgaagcagcc
    24241 caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca agcagaaatg
    24301 cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg tctgcctcta
    24361 ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac agaaggtatt
    24421 cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact tgttgtcatt
    24481 gcaggggtcg cctatctgct tcaaggatac atctcaacga tcggtattcc tgaagaacag
    24541 aaaaagacga tgcgtacgat gctaatcgtc tctcctgcta tgatcgtctt catgtcgatc
    24601 agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag ctgtctgcaa
    24661 acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc agaagagttg
    24721 aaacaaaacc cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga atcagcaaag
    24781 ccagctaaca acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac
    24841 aaaggccgca acgcaggaaa gcagcagaag cggaaataaa aacataggac aaaaaagatg
    24901 agagctaagc cctcatcttt tttgttactc ttcctcttct gctattccat taattaattc
    24961 gttgacagag acaatgcttt cacatcctgc acgaacaaac tcgggctctg aaccaatcat
    25021 ttgtgagttg ataaattcaa tgaccatcgg aagattcatc ccagcataaa gagtaatctg
    25081 ttgtccttcc attattagtt tagaaatgat atttgcaggt gtaccaccta aaagatcgca
    25141 tagtaccaca aaatcattta acgaatctgt gacagtgaga aacttttgtt tgtatgtttc
    25201 agtgccatct tctggtaata aaggtaacgt gtagatatcc tcttgaggcc ccatgatcat
    25261 ttctgtactt tttttcaatt cctcacaaaa ttttccgtga ctaatcaata ccaattgtct
    25321 actcattttt cgctctccct tatttcgaaa tcacacccaa tgcagataaa gcaacacaca
    25381 caattaatac gataaaaatc gctcgagtag gagtcatgtt tttctttcct aataaccaat
    25441 aaacaccacc gacgatccca gcaggtaaaa ttttaggtaa gatcatatcg atatagtttt
    25501 gcatattcaa tgtcacatcg ccaatcgttg gtgcccatga aaatttaaca ttgatcattg
    25561 tcgcaactaa tgcccctacc atgaataccc ctaataaagt ggctgcgtcc gttaatgcag
    25621 ttaagcgaga ttgcatagta gtaactaagg aaattccttc tttgtatgca aattcaagtt
    25681 gcttccaacg aaaaaccatg acaacgatct gagcaaaaat ccatataaac acacctatcg
    25741 gatttccatc agttgccata ttcgccgcca aagccccaaa aatcgttgga atcaaagcag
    25801 caaatacgga atctccaatc gctgcaaagg aacccatcaa tccagctttc agccccgata
    25861 cggtttcctt tgaagcaatt ccctcttttt cttccattgc aagatcaatc ccagtaatga
    25921 ttgtattaaa aaaattagaa gtattaaaaa attgcgtatg tgttttcatc atctctttta
    25981 attctggtgt attgtctcca tatatttttc ttagttgtgg caagatagta tataaatagc
    26041 cagaaccctg cattctctca tagttccaac ctaattgaaa agtgaacaaa cttcttcgat
    26101 tgatttgctt aaaatcttct tttgttaatt tgtaattaga tttcgtcatc ttcgatttcc
    26161 ccctcaacag aatgtgatgg ctcactagct aatggttgat ggtctttgtt ttgatcattc
    26221 ttataactga tcattgctag tgctagacca atcaatgcta cagcaagcat cggcagcgaa
    26281 ttgaaagttg ccggaagatc tttaacaacg cttgccacac ttgttcctaa taattgaaca
    26341 ttagagaata acgttgctaa taatgccgtg atcgtaaatc ctaaaataag ataagataga
    26401 tgttttttta ctggtaaata gcgcagaaga atggcaaacc caacagctgg taaaacagct
    26461 cctgctactg aaagtccatc gcctagccat tttagattcc cgtttaaaac agaaacaaca
    26521 ttttcaacta atccaccacc aaatgctaaa gctaaaaata caggaatcat acgagagagc
    26581 gaccaaggta aagctcccat taagaaattg cgttcgattg cttgataatt catgtcttct
    26641 acgtgacggt caatacgatg ggcaaagtat gtgttagcga atctagctaa aatatccaat
    26701 tggatcatta agcttgctac aggcactgca atagcagcaa tcgcttgttc tggattcatt
    26761 ccaagagaaa tcgagaaggc agttgctaaa atcgtcccag agttggcatc tatttttgat
    26821 gcgcctccaa acgtccccac tcctaaaact gtcaactgca tactgccacc aataatcaaa
    26881 cctgcgttta aatcacccat tactaaacct gcatataacc ctgcaaacac tggcgcactc
    26941 attgaagaat agatctgtaa ttcatctaaa atttgataac ctgcatataa tgttaataat
    27001 aagatctgcc accattgaat atccatggat cattcctcct atttgattaa aagattcata
    27061 aagtcttctg ctttatcgtt tggtaccatt tgagcaatta aatgtatacc aagctgatct
    27121 aattttttaa atacttcgat atctgcatct accacattga ttgattttgt aatggaccgt
    27181 gtttcattcg tttgtgacat attccccaca tttacttctt tgataggaac tcctttttct
    27241 actaaagata agattcgatc tggctttttt gctacgatca atactcgttg agagtcatat
    27301 ttgccagcta aaatatttgt tgctgctttt tcgattggca aaatgcttaa ttttacccct
    27361 gctggagtcg ctaacttcaa tccacttttt tcaattgtgt ttgtggctac ctcatcatcc
    27421 acgaccatga ttcgactaat attcagtttc gttgtccaaa gatttgctac ttatccatgt
    27481 atcaaacgtc catcgattct tacaccaata attgccatca tgcattctcc ttatctattt
    27541 tttctattta ttgttcaatt aaaactgtga catcatcaaa tatcccttct gtttcaaaga
    27601 taacgttttc attttttctt tcttatattt catttttaag ctaagaacaa attccttccc
    27661 aactaaagaa aacgctttct tttatattta agttcatttg attttcttaa cttgaatgtg
    27721 tttcatcaac atcgtaactt tcatagggtc gatccagcca gttttctctt gttgcgcgtt
    27781 tgccatacca caagccatag aaaatgcgag aatctcttcg attgattgcc tttttgataa
    27841 cgcatgggcc gatcctgcaa gggtcgcgtc acctgaacca acaggattaa caactgatat
    27901 tttaggaata atgacttcgt aaaaacactg agcaactttt gccacagcac ctctctctcc
    27961 taaagaaaca acaacccatg gcactccttc aaatattgga tgagctaata aggactgaat
    28021 tttttctaat tgaatcgaag aacagatacg ttggccaatc agttctccta attcttccag
    28081 attgggcttg ataagtagtg gcttctcctt accttccaat acttcaagca aaaatttacc
    28141 agaagtatct agtaatatgt ctttcccttt tttatcagct aaagcaacca attgcttata
    28201 gtaatcaaga gggcatcctt ggggtaaact acctgagatc gtcataatat ctgatatttc
    28261 gaggagctgc tcgaaatgat cggtaaatgt ctgttgttct ttctttgtta tgaaggggcc
    28321 tcgttccaaa agctctgttt gtctcgaact atgcaggata gcaatagaat cccttgtctc
    28381 tcctttaatt ggaacaaact gatgcatgat ccctaactga tctagctggt tctttagata
    28441 ttcaccgtgc tggccaccta ttaaaccagt agctatcacc tcttctccta ataaatttag
    28501 aaccctcgcc acattcactc cttttcctcc cgctgtttta ttgactgttt caactcgatt
    28561 gactttgtcg aggtttaatt cttcaattgt atacgcgata tcgatggaag gattcaaggt
    28621 aactgtcgta atcatttcat cttcaccttt ttgctttttg ttttattgaa ccaaggggta
    28681 gcggtttcat ttattacttt atttaaagtc tcgatgtttt ctctcccatt tgttcttaac
    28741 caatgaatag ctccgtcttt tccattttcg acgtatgctt tgaccccatc tttccatgtc
    28801 gcacgtccac acaatacacc attgaaggtg gaacccgatt catgagcaaa aatcagtgtt
    28861 tgttgaaata attctgttga aacaccccca ctcaaaaaaa tgaatggtaa tggagtcgct
    28921 tcactttgct ccttaaagaa ggcagcagcc tcttctttgc tgtgaataaa ttcacctgtg
    28981 gaatagccat tcacataatt catattgact ggtacttcta ctttcaacac atcaacttta
    29041 tattttggat cagaaaattc tttcatcatc tcaataactt tgtggggctt gactttcgca
    29101 tactctggag aagaactatc gcttatttgt gcgtcataag aaactaactc caaatagaag
    29161 ggacgatttt ctccttcgca ctcactacct aaacgttcga tgaacacatg cttcaaatga
    29221 ttgatttcgg gtgcttcatc aacatcgtag taaagtaaga attttactgc atccgcttgc
    29281 tgttctttca atctgagaga agaccaatcc gctaataaat caggtagacg accaggcgta
    29341 gttgcgtcat agccggtttt ctcataagct aacaataacc ccgtctcttt tgctctgact
    29401 tttgctgctg gcaatccata ttccggatcc aataaaatag cagaagcata gggtgtcagc
    29461 tcagctgata ctagttcttt gaattcactg atttggtctt cacttggctc ttttcccaac
    29521 gatttgatca ttttttttag agcaccacgc tgatcgatcg ctaatgcact aataatccca
    29581 tcttttgttg atagtctctc cattgcccta tttttttctt cattcatctg tcatttctcc
    29641 ttcggtttca tacttatgaa taatgacgcc cttgactact cgattgaccg tgcctgttgg
    29701 agatggtgta tctggtttat tagctacttt gattgaagta tagagtgcaa tgatttgtgc
    29761 agtcataatc atcgctaatg caagataggc atcgggcaat ttactcaccg agtcttccaa
    29821 tagcacagtc atacctgaga aatcaatagt acccggttgc gcaatcgcaa tgacattggc
    29881 agtgacccga tcttctttta tttcctctaa aatatctaag tcataatttc gcgtatatgg
    29941 ttgattgctc acaaaaccta caacgagtgt ctcgctattt aaaaaagact ttggtccatg
    30001 acgaaacccc atcgaagaat caaaaattgt agcaacttta cctgctgtta attctaaaat
    30061 ttttaattgt gcttctcttg tcgcacctgc caaacttcct gaacccagat aagcgatccg
    30121 ttcaaaaaaa tcaggtaata acttttgaat ttcttgatct cttgaaagaa gatcttgacc
    30181 gattttaact aattgttcaa gatatctttc ttttttctcc attgctgttt gatcaaagat
    30241 ccataaagca ctcaataaca tacacgtaaa acttcctgtc atagcaaaac ctgcatcgtt
    30301 tgctctctct ggcatcaata acagataatt ctgtttatca tcctgtgcgt tttttgccaa
    30361 catcccctct ttagcacaag taatcgtcaa atgaaacaat gtttcaactt gttcatttgc
    30421 aatttgcaca gccgctacac tttctggact gttgccactg cgagcaaatg aaactaataa
    30481 agtcggttca tttggaaaca aaaattcttt tggtgaagct acaatatctg tcgtgccaaa
    30541 actgtggaag ataaaaaact gattgtctcc atgttcattt agatagggta agagcgtatc
    30601 cccaatatat tgagacgttc ctgcacccgt aaaaataaca cgtattcttc ttccgtttgc
    30661 ttcttttttt atttgatcaa aaaatagagc taaattttct tggctttgca tcaatctatc
    30721 gattgtttct ttccagagat ctggttgttg ttttatttct tgtgtcgtga ttgctgcacc
    30781 tatttgtttt aatttgttct tcgatagctc aaacatgttg ttctccctct caatcatttt
    30841 cggatatgcc gaattttata atgaaactgg tccgctcttg ctacactatc tgtatattcg
    30901 ataatctcat ttttcacgtt ataggttatc ctcgtcagat gtaagacggg agcgccatca
    30961 gcgattcgta gcacaacagc atcttttttt tgcacgatac ttgcataaaa ctcttcatca
    31021 gctaattgaa cttgttgttc ataatcctgt gaaaacacat catacaacgg tttactttca
    31081 agtaacggca aatcaagtcc cataaatagt ttcgccggta gataagaacg ctcaaccatc
    31141 aatggctctt catctgccaa acgcaatctt ttaagtttaa atacagtttc acctaaatgg
    31201 atgttcaaat tcttagcaat tctcttatta gcttccattt gtctaaattc taaaatgacc
    31261 gtcttaggga cgcgccctat ttcgcgcatt tcttctgtaa agctatatgc tccagctaag
    31321 ttcgatgact tatcagacaa atcagagaca taggtacctt ttccatgtct tcggtaaata
    31381 taaccttgat tttccaattc ctgtaaagcc agacgtacgg tggtacgact caccccatag
    31441 tgttgacaaa gttctctttc agataacagc ttatcatgcg gagtcatctg tgtttcgata
    31501 cgatccttca ataaatcaac tagttgataa tacaatgatt ttcctttatt tgagctctcc
    31561 ataaaaatca cctcttcttt actggttata accacaacac aagaatagtg aattattaat
    31621 catttgtcaa aggtttttta gaccaaaaaa gctattataa cagctcttta aagctttata
    31681 tcttgaatag acaaagatac ctcaaaaata ccagttacca ataaaagaac agaaaaaaag
    31741 acgaaccaca taagcgatcc gtcaaaatgg tctaacttat cgagcagcgt gatccacatt
    31801 aatccctatg atattcctac aactatcgtt ttattacttc caatatcatt tacaacaact
    31861 aaaaatagct tattaatgac aaatgttatt attagtttta gataactttt gcagttttat
    31921 actttttttc tataaaattt gacaaacatt tttttaaata actaattatt ttttgaatct
    31981 gtcactcaga caatcctttc tcttacttgt taacataaat agaagttgct gtttcttcta
    32041 atagtgtact taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt
    32101 tttcttcgcc ttcggtaaca aaaggtgcta ctcccttcgc ccatgttgct cgcccacata
    32161 agacaccatt gaatgtcgaa cctgattgtt ttgcaaaacg caatgtatct tgaaatagtg
    32221 cagcgctgac tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat
    32281 agaaatacgc ggatgcctct gcttgactat aacaagtatc ttgcccatat ccttccacaa
    32341 atctcatatt cactggtact tctactttca atacatccac ttgataacgt tcttttgaaa
    32401 attctcgcat ggcttcaatc actttatgcg gttttacttt tgcatagtca agagaagacg
    32461 gatcgccaat agttgcatcg taagaaagga gctctaaaaa gaaaggtatg ccttctgctt
    32521 cgcattctga accaatccgt tctataacta tttgtttttg cacattaatc tctactggtt
    32581 catcaacgtc atagtacaat aaaaatttac aagcatccgc acccacttct tttaaccttt
    32641 tcactgacca actattcagt aaatctggta gtcggcctgg cattttcgca tcatagccgg
    32701 ttttttcata agctagcaac aaaccacatt ctttctctcg aagcgctgct gcaggcagac
    32761 catattctgg atcgagtaaa atagcggaag catacggagt caggtactga gaaaccatct
    32821 gtttaaattg gatgatatct tctgatgtag cttctttata ttggttgatc attttcttca
    32881 aagcaccacg ttggtcgata gctagcgcgc taataattcc agcttcatcc gtcaatcgct
    32941 tcaaacgttc ctgtttttct atactcattt tcttcatata tcttcaccta tttctttagt
    33001 taatattgta gctatttcta tttttgattt tgcagctctc aactgttctg taacctcttt
    33061 tcgcatcaat gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga
    33121 atctgggata aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc
    33181 aattcctttt ttcagtgtga cgataatcaa actaggttca ttgattgttt gagacttcgc
    33241 atgagggata gcaaaacctg acattattcc tgtagtacct tctttttctc tttccagaag
    33301 tttttgatat acttcttttt cgtcatgagc gattcctaaa cagaatgttt gctttgctaa
    33361 aaacttaaat gcttcttctt gattatctaa tggctgttgt aaaaaaatgt tctcttcatg
    33421 aatgattgtc atgataaacc tcctgctctt ccttcttcga taactgtacg atttgttgct
    33481 ttattatccc tcttcttgtc tttatcagtt actcttttct tttggatcaa acctagtatc
    33541 actccaccaa cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact
    33601 acaggtaaca ctaaaaaacc gccatgtggg gctggtactt ggacaccaaa cagataggtc
    33661 aagacagcag caattgatga gcctaacatc atcgttggta atactcggat cgggtctttc
    33721 gcagcaaacg gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa
    33781 cctgcattac gatcattagt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta
    33841 atcaacggtg gtgcaatgca agctgctgac gcaccagcca taaaagtcgt gtttccttgt
    33901 gtcaaaagag ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac
    33961 gcacacatac agccaacaat cacgccaaga acaagtggat tcgaatattc aaatccagct
    34021 aaaaagctca tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg
    34081 atgccaataa atgctacacc aataactgga tataagacga ttgccttcaa gccatctagt
    34141 tgtttaggta aaattatcag tagcttttgc aaaaataaga taaaataacc tgctaagaac
    34201 ccagctaaaa tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca
    34261 aatccaacga ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact
    34321 ggaaccatca ttcccattgc tgcatcgtca actgctttta ccattgccgc tgtagcattg
    34381 tattgtgcat gatctggatc agaagaatag attccccaaa cggcaaatga aatagcaatc
    34441 aatactccgc cagctaccac gaaaggcaac atatgagaga caccgttcat caaattttta
    34501 tagatgctac gaccaaaatt agcaccactt atcgcttctg gttctaaagt atcagcagct
    34561 tcattatctg ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca
    34621 tccttgattc ctgtactgac agaaacatca atgactcgtt ttcccacaaa tctttctgaa
    34681 tggacatctt tgtctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt
    34741 aattcattct cgatcccaat ttgtccatgg gtctccactt tgatcgtgat gccttttttc
    34801 ctagctgctt gctctaatgc ctcttgcgcc atgtaggtgt gcgcaattcc tgtcggacaa
    34861 ccggttgctg caattagttg atattttccc atctttcgaa cctctcttcc ttgattttga
    34921 tttgtttact taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcagtag
    34981 agctgcctgc tgctatgctt ttacgtaacg tttcatcaag cgatcgtcga ttcatgtatc
    35041 ctgctaaaaa agtgcctaac atggcatcac ctgcacatgc tgtattgaca acttttcctt
    35101 ttggcgagtt tcctcgaatc accctgtctt tcgtaaaaag aatcgctcct ttctcaccga
    35161 gggataacaa aacattttct gcgcctgact ctactagtcg ttgtccataa acaaagtaat
    35221 cttcatctgt aaccattttt cgtccgaacc aagaagaaag ttcttcctca tttggtttca
    35281 gcaaaaacgg tcgatagggt aaacaatcca tgacttcttg atcactacta tcaataatca
    35341 aacaaatccg actgatttct attaaaacct ttggagacag ccctcttggt aaactcccag
    35401 aaacacaaag ataatcccct gcttgtaatg ctcttatttg atttaaaaaa ttatgaacag
    35461 ctgtctgcgg tatttcaggt ccttgattca ctaatttgta ttcttctccc gtttgattga
    35521 cttgtgtaaa tacattgata cgcgtcatac caggcacttc aataaactct gtttggattt
    35581 gtttctgctg taaatagtct tctatatact ttcctgtgaa cccagcagaa aaacctaatg
    35641 ccgtacttgg gaaatctagc atctttaata ctagtgacac attcacccct tttccgtttg
    35701 cctgaatgtc atcctctttt gtgcggttta ccataaaagg atgcatctca tctgtttcaa
    35761 taaatagatc aattgccaaa ttcatcgtgc atgtgtagat agacataacc aacgacctcc
    35821 ttgattctat tatgaaatac gcgaaataga aaatgttttc ttaatcgtcc caacacaaag
    35881 aaaaccttta catttataaa ctcgtcgtgc tttttttaac taacttttgg tgaaagaagg
    35941 aaagaatatg aatcacaaat taagtgaaac agagcagtac ctctggaact ttatcgaaca
    36001 cctcatccta gagatcccta actattccat cgtgaaacta agtgaacaag caaatgtctc
    36061 aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaagggttta cttcgtttaa
    36121 gcatcatcta aaagaaggag aaaacaagaa catcaatttt tcatttttag ataaagtaga
    36181 caaaggaatc cgccgagcca ttctaaaaaa tgaacaagaa gtggtacgta caatcaatat
    36241 gttggaaatc gggaacattg aagatgcaat acaaagaatc aaatttgctg accgggtttt
    36301 tgtctttgcc cgaggatttt cagaaatgat tggccaagaa atgctggtaa aacttcaatt
    36361 aacgggaaaa aattgtcaga tgcatactga tccagaaatt atcaaaagca tcagtcggaa
    36421 actaactaag aaagacagcg ttatttttgt ttctttgaac ggtgagaaaa caaataaaaa
    36481 catcaaaaat aagatttatg tctttatttt tcaatttttt aaat
//
LOCUS       DASJRQ010000016        35306 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJRQ010000016 DASJRQ010000000
VERSION     DASJRQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.016"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..84
                     /locus_tag="U4A80_000730"
     tRNA            12..84
                     /locus_tag="U4A80_000730"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="U4A80_000731"
     tRNA            88..160
                     /locus_tag="U4A80_000731"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="U4A80_000732"
     tRNA            168..249
                     /locus_tag="U4A80_000732"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="U4A80_000733"
     tRNA            267..339
                     /locus_tag="U4A80_000733"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="U4A80_000734"
     tRNA            351..422
                     /locus_tag="U4A80_000734"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="U4A80_000735"
     tRNA            439..524
                     /locus_tag="U4A80_000735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="U4A80_000736"
     tRNA            535..608
                     /locus_tag="U4A80_000736"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="U4A80_000737"
     tRNA            618..691
                     /locus_tag="U4A80_000737"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="U4A80_000738"
     tRNA            711..783
                     /locus_tag="U4A80_000738"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="U4A80_000739"
     tRNA            811..884
                     /locus_tag="U4A80_000739"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="U4A80_000740"
     tRNA            898..971
                     /locus_tag="U4A80_000740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="U4A80_000741"
     tRNA            995..1084
                     /locus_tag="U4A80_000741"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="U4A80_000742"
     tRNA            1097..1170
                     /locus_tag="U4A80_000742"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="U4A80_000743"
     tRNA            1189..1264
                     /locus_tag="U4A80_000743"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="U4A80_000744"
     tRNA            1266..1336
                     /locus_tag="U4A80_000744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="U4A80_000745"
     tRNA            1350..1423
                     /locus_tag="U4A80_000745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="U4A80_000746"
     tRNA            1448..1535
                     /locus_tag="U4A80_000746"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="U4A80_000747"
     tRNA            1557..1628
                     /locus_tag="U4A80_000747"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="U4A80_000748"
     tRNA            1651..1740
                     /locus_tag="U4A80_000748"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="U4A80_000749"
     tRNA            1753..1826
                     /locus_tag="U4A80_000749"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="U4A80_000750"
     tRNA            1845..1917
                     /locus_tag="U4A80_000750"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="U4A80_000751"
     tRNA            1921..1993
                     /locus_tag="U4A80_000751"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="U4A80_000752"
     tRNA            1998..2068
                     /locus_tag="U4A80_000752"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="U4A80_000753"
     tRNA            2081..2154
                     /locus_tag="U4A80_000753"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="U4A80_000754"
     tRNA            2166..2237
                     /locus_tag="U4A80_000754"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="U4A80_000755"
     CDS             2503..3696
                     /locus_tag="U4A80_000755"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1974390.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="U4A80_000756"
     CDS             3826..5298
                     /locus_tag="U4A80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1974391.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="U4A80_000757"
     CDS             5381..6169
                     /locus_tag="U4A80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1974392.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="U4A80_000758"
     CDS             complement(6236..6904)
                     /locus_tag="U4A80_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1974393.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="U4A80_000759"
     CDS             7019..8731
                     /locus_tag="U4A80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974394.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="U4A80_000760"
     CDS             8734..10506
                     /locus_tag="U4A80_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974395.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..11709)
                     /locus_tag="U4A80_000761"
     CDS             complement(10588..11709)
                     /locus_tag="U4A80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1974396.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(11781..13061)
                     /locus_tag="U4A80_000762"
     CDS             complement(11781..13061)
                     /locus_tag="U4A80_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN1974397.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(13152..13604)
                     /locus_tag="U4A80_000763"
     CDS             complement(13152..13604)
                     /locus_tag="U4A80_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1974398.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(13617..14027)
                     /locus_tag="U4A80_000764"
     CDS             complement(13617..14027)
                     /locus_tag="U4A80_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1974399.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            14302..14514
                     /locus_tag="U4A80_000765"
     CDS             14302..14514
                     /locus_tag="U4A80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1974400.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            14517..14729
                     /locus_tag="U4A80_000766"
     CDS             14517..14729
                     /locus_tag="U4A80_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974401.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            14869..15024
                     /locus_tag="U4A80_000767"
     CDS             14869..15024
                     /locus_tag="U4A80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974402.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(15021..15245)
                     /locus_tag="U4A80_000768"
     CDS             complement(15021..15245)
                     /locus_tag="U4A80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1974403.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            15323..15511
                     /locus_tag="U4A80_000769"
     CDS             15323..15511
                     /locus_tag="U4A80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974404.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            15508..15681
                     /locus_tag="U4A80_000770"
     CDS             15508..15681
                     /locus_tag="U4A80_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974405.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            15837..16172
                     /locus_tag="U4A80_000771"
     CDS             15837..16172
                     /locus_tag="U4A80_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974406.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            16405..17346
                     /locus_tag="U4A80_000772"
     CDS             16405..17346
                     /locus_tag="U4A80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN1974407.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            17348..18238
                     /locus_tag="U4A80_000773"
     CDS             17348..18238
                     /locus_tag="U4A80_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN1974408.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            18281..19081
                     /locus_tag="U4A80_000774"
     CDS             18281..19081
                     /locus_tag="U4A80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1974409.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            19096..19947
                     /locus_tag="U4A80_000775"
     CDS             19096..19947
                     /locus_tag="U4A80_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1974410.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            20148..20609
                     /locus_tag="U4A80_000776"
     CDS             20148..20609
                     /locus_tag="U4A80_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1974411.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            20635..21087
                     /locus_tag="U4A80_000777"
     CDS             20635..21087
                     /locus_tag="U4A80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974412.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            21193..21387
                     /locus_tag="U4A80_000778"
     CDS             21193..21387
                     /locus_tag="U4A80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974413.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            21418..21711
                     /locus_tag="U4A80_000779"
     CDS             21418..21711
                     /locus_tag="U4A80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974414.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            21741..22181
                     /locus_tag="U4A80_000780"
     CDS             21741..22181
                     /locus_tag="U4A80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1974415.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            22256..22723
                     /locus_tag="U4A80_000781"
     CDS             22256..22723
                     /locus_tag="U4A80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEN1974416.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            23470..23700
                     /locus_tag="U4A80_000782"
     CDS             23470..23700
                     /locus_tag="U4A80_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974417.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            23859..24692
                     /locus_tag="U4A80_000783"
     CDS             23859..24692
                     /locus_tag="U4A80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN1974418.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            24685..26100
                     /locus_tag="U4A80_000784"
     CDS             24685..26100
                     /locus_tag="U4A80_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1974419.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            26112..27641
                     /locus_tag="U4A80_000785"
     CDS             26112..27641
                     /locus_tag="U4A80_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1974420.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            27559..28611
                     /locus_tag="U4A80_000786"
     CDS             27559..28611
                     /locus_tag="U4A80_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN1974421.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            28613..28930
                     /locus_tag="U4A80_000787"
     CDS             28613..28930
                     /locus_tag="U4A80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974422.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            29057..29680
                     /locus_tag="U4A80_000788"
     CDS             29057..29680
                     /locus_tag="U4A80_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN1974423.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            29695..30585
                     /locus_tag="U4A80_000789"
     CDS             29695..30585
                     /locus_tag="U4A80_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN1974424.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            30608..30823
                     /locus_tag="U4A80_000790"
     CDS             30608..30823
                     /locus_tag="U4A80_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974425.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            30835..31176
                     /locus_tag="U4A80_000791"
     CDS             30835..31176
                     /locus_tag="U4A80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974426.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            31176..31547
                     /locus_tag="U4A80_000792"
     CDS             31176..31547
                     /locus_tag="U4A80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974427.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            31540..31935
                     /locus_tag="U4A80_000793"
     CDS             31540..31935
                     /locus_tag="U4A80_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1974428.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            31937..32314
                     /locus_tag="U4A80_000794"
     CDS             31937..32314
                     /locus_tag="U4A80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974429.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            32315..32923
                     /locus_tag="U4A80_000795"
     CDS             32315..32923
                     /locus_tag="U4A80_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1974430.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            32923..33264
                     /locus_tag="U4A80_000796"
     CDS             32923..33264
                     /locus_tag="U4A80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974431.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            33506..>35306
                     /locus_tag="U4A80_000797"
     CDS             33506..>35306
                     /locus_tag="U4A80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEN1974432.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAI"
BASE COUNT        11759 a         5562 c         7331 g        10654 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgtaa aagtttttaa atgtattaca taattatatg ataaattttt
    11341 ttcaaaatag tagtaatcta gcatatcttg tataagatta ctattaacgt tttttacaag
    11401 tacatcatct tttatatatt ttgaaattaa tgatagataa acctgagttg ttcgatagga
    11461 actttctttt acagttggct tgtatttttc atcccattta gtttttaatt ctccaaaagt
    11521 tagtggtaat tcttcatttc gttttatttt ttctgcgatt ttttcattca aaagtaagtt
    11581 agcttgattc caagcttggc gtgaattgct agataaagta acggacactt ttctcttttt
    11641 ttcagttaag ggatccgtat aacgttcggt gtacttaaat tttttgtcat ttattttttc
    11701 taaccacatt atttctattc ctttcgaata tacgttccta ttaattgtta aaagaaaaag
    11761 cccgaaggct ttttatatat ttaatctttt gacaattcta aagatcgtct atattgttca
    11821 gctttagcta attttgtaaa tgttactgtt ccattataat tttctaaaac aactttctct
    11881 atttcgtcta gtgaaacttt gaaaaattct tttctttgat taattttatt aacttcttgt
    11941 tttctaaaat gattatgcaa tttagtttcc aaagacggag cgtcctctga aaaaatcatc
    12001 gcatgaacgt caaattcaaa tggaaccgat gcactactca attcttttat tctatccata
    12061 ggttctaatc ttctagtcat accaatttta tatatgtctt caccaaaaga cccaatatta
    12121 ctaattacat acacataacc ggctctagta tttaattctc tttgtaatac attttcacgt
    12181 ataacttcta attcttttaa tttttcttcc agttcttgaa ttttatttgc atataattct
    12241 ttttccacat ctgcattagc tttttgcata tatgcgatta atttggaaat ttcagaattg
    12301 aattgacgtt gttctttatc aatacgtttc ttttcacgat caatttctct acgaactttt
    12361 tcctcttcta tcatctgttc ttttattgct ttttgttgtt ctttttcttc ggtaattttc
    12421 actaaataag agtaatgaag ttctagttct tgaagtttaa actctaaaag acccttcctt
    12481 aattcagcat tgtcattctt gaataaatta ttaattttat caaatatttt tatgattttt
    12541 tccctagtcg agtctatatt agaaatagtt agtttattga gtaatccttt cacttctgaa
    12601 tcaaatggaa tgagcatttt tttctttaaa ttatttttct ctcgtttatt taaaataaga
    12661 gggacagaaa cagcatcgtc aataatatat tccttttctt ttaattttaa caaagaaagc
    12721 ttatttttta tttcagaaga tgaagtttct tctatatttg aaacgataag tgaagaatag
    12781 acatttttta atgtttcatc atacttttga tttatttcat catttaaatc ttctttctct
    12841 ttttttaata aaataatatc attttttaac atagtaattt gtttatttaa atctgatagt
    12901 gtttcttcct tacttaatat aaatctttct ctatcatgta atttatttag tttttcatca
    12961 tttttttgtt ctaattcata agcttctttt tttattgtgt tagctttcga cagtttagtg
    13021 attcctataa ttaataaaat aagtccaatc aataataaca ttcaaaactc ctcatttctt
    13081 tgatataatt attgtagagt ttactcagaa atgaggaaag agtccatgtg aaagcatggg
    13141 cttttttatt tttaatgttg taatactaaa tgttcaagat tatgaggaag ccctaaatat
    13201 tcaataattt ggaactgact catatcagat aaatcttgtt catctattgt tctagttaac
    13261 atttcaattg caaacatatt agcttcagct tctataccat aaatcataca actaaggttg
    13321 tttgctctaa aagctgctgt gcaaatacct ttatggagtc taacgtgggc tatttcatgc
    13381 cataatacaa atcttttcat agaaggtgat aggttttgat taattaatat cacataacat
    13441 cttttattgt gtatcgtttg tcctaatgtt ttattcccta aatcagtttc aattaaatct
    13501 atatcagaat atttgattat tttatatgga ttaatcgcat cagatccaaa ctctacaaat
    13561 aatttttcta tttcttctaa tatccagtat ttgtttaaat gcataccaaa gcccacctat
    13621 tgacgatatt ttttaggagt gaattttttc tttgctttaa ctttggttaa ggcaagtgca
    13681 agttgaatgg catcgtataa tttttcctta tcttcatcgt ctaaaggttc accataataa
    13741 tttgtttcag catctgaatt caagccttca agtaattttt cagcttgtaa agcaatatct
    13801 tgtttatctt tttctgttaa atcataataa tggcgtttat cagtacgtcc taaaagataa
    13861 tcagttgata cattaaagta atctgcaact ttttgtattt tatctccaga tggagtactt
    13921 ttattccatc tagacatgct tccatttccg aaatttaact ttctttcaag ttctgccaac
    13981 gtcattgaac gttcttttaa taagattttt atacggtcta atagcatagg atatgcattc
    14041 ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt atctatttac aaatagagaa
    14101 tttgcagtta tactagtttt gtaagcaaga tatttacggc atacaaagac taaaagcaat
    14161 aaactaatag tctccccaga catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc
    14221 ttacataata gcatatttgc agtttttgtc aaacttatta gacaaaaata ttagaaaata
    14281 ttcgataagg gggtatgaag catgacttta aaacagcaag tattaattgc tttagtaaag
    14341 aaaggttgga gtcaaagaga gttagctcga cgaatgggta tatcaattac ttatttacga
    14401 gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga agaacttttg
    14461 gatatcaagt tagaagtaga ttctaatgat cagccatcag aacaggaggc gtaaaaatgg
    14521 tacaacgttt gagcaaagcg gagtttaaag agaaatataa gtattctgaa agtacttatc
    14581 aaagaagaat gaaggaattt aaaaaatcac gttttagtga ggggtataac gcagttactt
    14641 ccaaagaaat aacaattgat gtcgatttat atgaacagtt tagaacatgg cgttctaaaa
    14701 atcgatttag agttagagct gtttgttaat agctaaaaaa tctaaattca ccacttctga
    14761 aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt cattttggca
    14821 actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa
    14881 tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa
    14941 gggaaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt
    15001 cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca
    15061 cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa
    15121 atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa
    15181 tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt
    15241 cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc
    15301 aataaacgaa aggagaagtg atatggcata taccttgcaa caagaacatc aaattctcgg
    15361 tttgattaaa caacgcagga aacaattaca agatgaccgt gcagcgctta gaaaagccga
    15421 tgagctatca gataggcaag ctgaactaat tgcttctgaa cttgaggatt tgagaatgct
    15481 agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt tataggattc
    15541 attttggggt tattagtgat tgtagcgcac caaagtatta tcgggggaag cttgttcgca
    15601 gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga
    15661 agaagcgcta aaaatcggta aagtgattgc tgataatcgg tggggaaata gccgccctat
    15721 tattttaagc aagcaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact
    15781 ctgccagcca gcaaagagtc acatacaaat ttataaaact aggagaatta tatcacatga
    15841 ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag caaggtgttt
    15901 ctattatttg tacagcccaa aaagaagggg aacttaaaag tctcgtttat ggtgagacaa
    15961 cagaaatttt actttgtctt gctatgcaag aagaacattt agatgaaaat ctcccattgt
    16021 cagcacatat tatgcgtaga attgctgtgg atgcttacga acaagcgaaa aatgaagaag
    16081 aaaatcaacc ttcaaatcat acttttgtta ttaataacaa ggaagattta gctgatgtga
    16141 tgactcgtat tctcaagggg gaatttcaat gatgaatgaa aaaacaattg atctactcat
    16201 tattttattt ttcacgttag tgattgtagc gaaaagttat gtaatctttg gcttgttatt
    16261 cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg
    16321 agttagcaaa tcttgacaac tatttaactg atcctgatta tgctaaacca ccatatgaag
    16381 caccaattga tgaggaggat gaagatgagt aaatctacct tagaaatgag ccatcaagaa
    16441 tggcttgaag accgtaagaa aggcatcgga ggttcggatg ttggaacaat tttaggattg
    16501 aataaatgga aatctcctta tcaactatgg ctagaaaaaa caggacaagt agtacttgaa
    16561 gaatcaggaa gtgagcccgc ttattggggt aatgttttag aagaagtggt tgctaaagag
    16621 tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc aagtatttga acatccatta
    16681 catccatttt taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc cattctggaa
    16741 tgcaaaacag ccaatcaatt tctcggtaaa gaatgggaag gcgaagaggt accgcttagc
    16801 tatctctgtc aagttcaaca ttacatgaac gttctaaaca aagactattg ttacatcgct
    16861 gtcttaatcg gtggtcaaaa atttatctgg aagcggattg aacgagatca agagctgatc
    16921 gatacaatca ctgaacaatt agtagagttt tgggaaacaa acgttcttgg aggtatcgag
    16981 ccaattattg atggtagtca agcgacagct gatttcttga aagagaagta tgcagatgtt
    17041 gaagacgttc aaacgacgtt accaattcat ttcgatgaac tagttgaaca gaaaaacgaa
    17101 ctcaaacgga ctaagaaaga aattgaatca gctatccgtc aagtagacaa cgagattatc
    17161 agcgagctag gtaaacgtga agccagtatc ggtatcactc aaaaaaacat catcagttgg
    17221 aaatttgtcc gtacgagacg tatgaactcg aagaaactag cagagaaata tccagatgtc
    17281 gcaaatgatg aagagattta taacgttact gaatcaagaa ggctaaccga aagggagatc
    17341 aaataatatg gcaacaaatg aatcgttaaa aaatcaattg gcagcaaagc cacagaaaca
    17401 agttgcacca ggtcaattag gacttaaagc tttgatgaat acaccaacaa tgagaaagaa
    17461 gttcgaagag gtgcttcatg acaatgctaa tgcttttatg tcgaatgtta tgactcttgt
    17521 atctaatgac agttatcttg cagatagtga accgatgtct atcatgagtg gtgcgttaac
    17581 tgctgcaaca ttaaatcttg ggctagataa gaatttaggt tatgcatatt tagttccatt
    17641 caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca tgcttggcta
    17701 taaaggatat atccaattag cccaacgatc aggtaaatac aaagcattaa atgtgatcga
    17761 agtttacgaa ggagaactaa aaagctggaa ccgactgaca gaagagtttg agtttgatcc
    17821 aaatggtaga acgtctgatg aagtcattgg atatgttggc tatttcgagt tactgaatgg
    17881 attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa
    17941 taaagatcgc gataagacaa agttaagtgg tgtgtgggca tctgattaca atgcaatggc
    18001 acgaaaaact gttttgagaa atcttctttc taaatgggga atcttgtcca tcgaaatgca
    18061 agaagccacc acatcggatg agagagtcca aagggttcaa gaagacggca gcattattgc
    18121 tgaaacagaa gttgaagaag atattcctga aagaaaagaa gcagaggtta tatctgaaga
    18181 aaatgaagat gtacaaactg gattatttga tgcatctaat ccgccgttaa acaaataatg
    18241 agggagcttt ctcccttgct tttctagagg agaaatacga atgaacacag gatatgtcaa
    18301 attacatcga aaagtgatga attcattcgt ttggaccaat ccttatatgt ataaattatg
    18361 gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag atacttttta atggaaaaga
    18421 gatacaagtg aacagcggag aattcgtcac agggcgcgat gctatcacat ctgagatgaa
    18481 cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct gtatggagat ggctaaaaca
    18541 atttgaaaaa aacggaatgt tgaacatcaa atcaactacg aaatacagcg ttatatcaat
    18601 aaaaaactgg tctttgtacc aaagcagtga acaacaaatg aacatcaaac gcacaacaag
    18661 tgaacaacaa gtgcacacaa acaagaatga aaagaattat aagaatgaaa agaataataa
    18721 aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac accgactcag tttactacat
    18781 tctcgcggag gagttattca aacagatttg tcagaatcag gaaatcaaaa agccgaatct
    18841 gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca
    18901 ggtacgggga atgattgaat ggagtcagca caatgtgttt tgggcatcga atattttatc
    18961 tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg caagcaaatc gtgattataa
    19021 aacaaaacaa actaaaacgc ttgaatacga gaaatttagc acagatgagt tgcctatttg
    19081 agaggaggcg taggcatgga aactgttggg gaaatcatgg aaaagctgat acagaaagtg
    19141 cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag
    19201 aaccctgatg gctcagaacg ttgtaagcca acctgtatgc aatgtgggta taaagcattg
    19261 cgagttcagg aagacttaca aacagaacga atttacaacg agagcctgaa agcaagagca
    19321 atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga ttgcacattg
    19381 cagaattatc aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt
    19441 gttaattcag tcttgttagg aaatccaagt caccttgttt taactggaaa acaaggaaca
    19501 ggtaaaagtc acctagcgat ggcagcggct tgggaagtct tgaagcgatc aaactacgat
    19561 aaaaaaatct tatttatcgg gttacaggaa atgctggatc aaatcaaatt ttcgtacaac
    19621 aatcctgaac tcagaaaaac gattgaggga tcgttgattg cagatatcaa aacagcggat
    19681 ttagtcatca ttgatgatat cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg
    19741 tttggcataa acacgctaaa ttcgttcttg gatgcacgac agaacctagc aacgattatc
    19801 acaacgaact tgcttggtga agaactgaaa aaagcttacg gtacgagaac aatatcaaga
    19861 atgtttgtca actctgatgg atttacgatg gtattttctc aaacagcaga caagcgcata
    19921 aaaccagtca aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc
    19981 agataatgtt gcaaattata ggtgatcgtg acatggacga attatctcaa caagagagtc
    20041 gaatggttcg gaagataagt aagctaatta tttatcggga tacagaaaga tttctcactg
    20101 ttttaggtaa cgtctacgag aatccagagt tattggaggt agagtaattg ccaaaaatat
    20161 tagatgcatg ttgcggtagt agattgttct ggtttgataa gaacaattct gacgtaacat
    20221 ttatggattg tcgtcagcag tatgaagaat tatcaacagg tcatgtgatc aatgttgatc
    20281 cagatgtggt cgcagacttt cgcgatatgc cttttgaaga tgatgtgttc gatatggttg
    20341 tatttgaccc gccacactta agacatgtcg gcgaaaactc atggcttgcc aaaaaatatg
    20401 gaaaattaga cgaactttgg ccagaagaca tcaggcaagg ttttgcagag tgtatgcgag
    20461 ttttacgacc ttcaggaaca ttaattttta agtggaacga ggaacagata ccactttctg
    20521 atgtgttaga ggctattgga gaacagccat tgtttggtaa taaacgaagc aaaacacatt
    20581 ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca aaggaggaaa agtaatgaag
    20641 attgtattaa ataaatgttt tggtggcttt gggttgagcc atgaagcaaa aatggaaatt
    20701 tttaaaagaa aaaatattga agtattccca tacatcaata actttagcta tgactcagat
    20761 gatgaataca cgagatacac aggccaaaag ctaggttcga tggattttat ctactatttc
    20821 aaaaaagacc cgaaaatcga caaagttaca ggaatttata gcgaaattga ccgactgtac
    20881 ggaattgctg atgattccag tttttcaagt gattcaaaca gaggtgataa ggacctcgtt
    20941 gctgttgtcg aaaaactagg aagtgaggcc agtggaccat atgcaagtct taaagtcgtt
    21001 gatatacccg atggggctga gtgggaaata agtgattatg acggcgttga aactgcacat
    21061 tatggttttc aaacaggtag tatttagtcc actaatgaca actaaagagg aggcagaagg
    21121 atgagtgaag atgaatactt tgaacagtag tctattaacg acagagattt ttaactggaa
    21181 aggggtgtag tcatggacgc atcggaagta gtaaataaaa ttaatagtct caaagaggtt
    21241 ttcggagacg tcgaagtagt gataaacgca caaggctatg acaacgaatt ttttaagcag
    21301 attaccgaca ttagcattca gcaaggaatg gaagacgaag acggaaactt tatcgatgaa
    21361 gtagccattt tagtgacttg cgagtaagta attgacagct aaagaggagg aagcggaatg
    21421 aaactaaaag acggatttta ctccagcggc cacggcatcg gcggtttaat gctagatatg
    21481 ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca aagtcggtga catggttcgc
    21541 tgcgaagcag aaggattcat ctatccattt cgtggatatg tagaacacgt ctataatcac
    21601 tcagcaatca ttcgcattga aaacacgatg gaatgtgata agtggttagc gaaaagcaaa
    21661 gagaatttag cagtggtgag attggtggac atggaagtta taaaatctta attttgcttt
    21721 tttacaacta tcctacaaca atgagcgtta ttccacaact ggaaggagaa gaggcaattg
    21781 tgaacatttt agagaatata gatattaagc aaacaaggaa aaatgctaga cgattactaa
    21841 aaagatacag aaatttagaa cgtttggtcg gaccagtgaa aatagacttt tccgtgatga
    21901 ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa cgaagaaatc attgaagcga
    21961 taagtactcg agattcggtt atcgaagcat taacacggct aagtagaatc catttccagg
    22021 tactttatta tagttattgt tttcctaata agatgtcaat gtatcaaata ggagaaaaat
    22081 taggatattc cgatagaact atcgagagaa tgaaggcagt agctttggtt gaatttgctg
    22141 aggcgtataa atcaggagaa ctcatttcac gtacaaaata aaaaaagtcg gattcctccg
    22201 actatgaata atatttccga cataagtatt atatcataat tgggggaatc agaggatggt
    22261 acttttcgat gtaaagaaat atgagacacc aagcgcgaaa gatgttgata tggaacgcac
    22321 aaaacataat gtcgctgtgt ttctttcagc atatctatca gctagatgta gagtagggca
    22381 acatcgagaa ccaaaagtga cagcatctta ttccttggtt ccaccttcta cagctgatca
    22441 tatatttgaa gcagaaagaa tgctgatcga taaagaagaa gcacaggaag aatttgagta
    22501 tctgcataaa ttgtttgttc gaggctattc agcgatacag catccgcata agcctgatgt
    22561 gactgaaagg cgcaagaaga tattctatga tcgttatatc aatggtctgc ccatttatgt
    22621 aactgctcaa aggaataata ctagcgaaga atcggttaaa gtagaatcaa acagaattat
    22681 catccaattt gcttcatcgt tagaactggt tgctttcaag tagccagttt ttacactttt
    22741 tatacccttt tattaccaat ttggtttcca ttttatacct tttttatacc aatcacttac
    22801 ctattcaacg ttgtattatg atagtgtcga aaaattagga aacaggatcg acaaaataaa
    22861 atgtaaggga ggaaatctcc ctcatcgttt aattaagctt cgataggcag caacggaaat
    22921 attaagaata aggatgtgaa tttcaactcc ttctaaattg ttcttattat ctatcatccg
    22981 ttgctgtcta ttgttttttt aattattcac acgataacta aaggtaagtg aagagaaatg
    23041 attccattaa taatttcaat ttttgcgctt tgtcttaatg tatatatgat tggatttaaa
    23101 aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga
    23161 aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc aaggttcgat
    23221 tccttgccag tgactttggc aaccgaggca tcggcggttt aaaaatatag gggtgcgcaa
    23281 tttcgtacgc gttttgtgca tcgtgtaggt tgctattaca taactggtta ggttagattg
    23341 agatttggga ttcggtacaa atgaatcgtc aaatgtctca agcacaggat cggaaacgtc
    23401 cctgcctgtg cattacatat tagatcactc tttgagtggt ctttttcttt acataaagga
    23461 ggctacataa tgagaaacta ctggtatgta tcgctaacta atgaatatcc tcgaaccatt
    23521 gatgattgtt cagtgcgtgt tgtgcgttct gtacaaatca aagggaagta ctctattgtc
    23581 gaaatgggga gagaagcaga accatgtgag attgatgcgt gcaagctcgt ttattgcggt
    23641 catggtttct atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag
    23701 aatgcaagaa atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg
    23761 atttaaagat attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa
    23821 aactcaacct aaacagaatg tgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa
    23881 gacgtgatga agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa
    23941 ttgcatctga attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat
    24001 ggagcgctga attgattagt aacgttacca atggcaaaag taacgttact aatcaaggtg
    24061 gcgctcctat tggtaatcaa aatgctaaag gtaacaaagg aaatagccga gcttctccgc
    24121 cagtgggtaa taaaaacgct ttgaaaacag gtgagtatga aacaatattt tttgacacgt
    24181 taaccgataa tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt
    24241 ccgaagaaat acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag
    24301 ctgaagctgg actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa
    24361 ctccgattga aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc
    24421 agattagcag aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga
    24481 ctcggattag caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg
    24541 aatacagaac tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa
    24601 ttggcggaaa taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg
    24661 caaacaaacg ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt
    24721 tctgccattg attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg
    24781 aatcctgatg aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct
    24841 gttcgttctg gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc
    24901 ttaacgtgtc gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac
    24961 gatgtacttt gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg
    25021 aagtggacaa agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct
    25081 agaacagcga ctaaaccaga aagtatgcaa ggttttcacg aggaccatat gttgattgtg
    25141 gtagatgaag cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca
    25201 ggttttgata ataagctgtt gatgtgtgga aaccccaata atattgaagg tgtcttttat
    25261 gattcccata attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt
    25321 aaacgtacaa acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat
    25381 gttgctcgtg tccgtatttt tggagaattt ccgaaaggtg cgttggattc atttatcagt
    25441 cttgaaacag ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca
    25501 aaggttgctc atattggtgt cgacgtagct cgatatggtg atgattctac gattctcttt
    25561 cctagaattg ctaccagggc attggagtat gagaagtatt caaaacgtag caccatggaa
    25621 acaacaggat atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat
    25681 aaagtgatga ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa
    25741 gaacttatag aagacaaaca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca
    25801 tcagaagacg atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg
    25861 ttagaagaaa atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct
    25921 gatagttcgt taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt
    25981 atacgtttag aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct
    26041 gacgcactgg ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag
    26101 gaggtgagct tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta
    26161 aatatgcaac acaaattgaa gatggaatgt ttgatccgaa tggttttata gaagatatga
    26221 aaccattttt tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa
    26281 tagataatag accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg
    26341 gtatgtattc aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat
    26401 gggattacga tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct
    26461 taaaaaatga tatagaactt ccgacagttc cagaagcttt taacaaatta acaagcaatc
    26521 ttgattccat gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg
    26581 gagtagcttt ccgattgcta gagcctgtgg aagaagatga tggttggaag ttaagagcaa
    26641 gtaatattga accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa
    26701 agtatgactc ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa
    26761 ttctgacata tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa
    26821 ttgatgagac cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga
    26881 atcgttattg tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt
    26941 ctgatcaaca aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa
    27001 caatgagcaa aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag
    27061 cccctactgc aaaagctgaa tatgtcacaa aaaatttagc aaaagatttc aatgaatatc
    27121 atatggatct attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg
    27181 aggtgtttaa atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag
    27241 cgaaaacaaa tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa
    27301 ttgctgcttt tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta
    27361 ctttctctag atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg
    27421 caccatacgt atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt
    27481 atgaaaatga aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt
    27541 ttggcggagg tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg
    27601 gaggatcaag caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt
    27661 cctgaagcac tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc
    27721 acttatccag aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat
    27781 gtggaaaaaa attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat
    27841 gaattttttc caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat
    27901 gtatttaaaa ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat
    27961 cgcttagagg aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt
    28021 ttaaacgttg atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat
    28081 ccatggtcag gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag
    28141 aaactctcta atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta
    28201 aacaccatga gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat
    28261 aacaaagcga tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt
    28321 gaaaaatcat atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag
    28381 agagtttgcc ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct
    28441 gtcataggtg aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat
    28501 ttaccaaaat taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta
    28561 gatgagtttg cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga
    28621 tttttttgaa gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac
    28681 agctattgag tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc
    28741 ctacgtggtt attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa
    28801 taccgttaat ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt
    28861 tgattggtat gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc
    28921 tagtaattag gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt
    28981 ggttgaatgg tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc
    29041 gtggggcgaa aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata
    29101 ctttgccgaa ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc
    29161 caatgaagaa gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt
    29221 ggaagcttgg aaacaaaata acttagataa aattaaacag gataccatca aggaatatga
    29281 ggaatctaag aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt
    29341 tgaagcagaa aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat
    29401 tgctggatta gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa
    29461 tcttgttagt tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt
    29521 gaatgcaatt aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt
    29581 tggtggaact caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc
    29641 agaagaacaa ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa
    29701 aaaacatctt taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa
    29761 gtaccaaata cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac
    29821 tcgacagaaa ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt
    29881 gagggcggag aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa
    29941 atttttagaa aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc
    30001 ggaaatgaat taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac
    30061 cgaaaattga ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat
    30121 gggattttga acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta
    30181 acaagaaaag atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa
    30241 aaactatgct tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa
    30301 tcaacaaaaa ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc
    30361 gtgtctaatt acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt
    30421 gccgcaatcg atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa
    30481 cttgcaaaaa caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac
    30541 aatgcttcaa actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact
    30601 cccttttttg ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt
    30661 cgtgaaatat agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg
    30721 tagatttatc acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc
    30781 aattgcaatt ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat
    30841 gaatcgctaa aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa
    30901 gaacgcataa ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt
    30961 aaaaatgata tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata
    31021 ataaaaaatg aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt
    31081 tatgcttcaa caacaaatga tgttagtgag ttacttctag gctatggtga tttgatacga
    31141 agagttattg gttgtggagg attggagttt ttttaatgaa tgaagcagat attttagaaa
    31201 ctacttatga agatagttgt attattgaaa gactgacgga cattgaagat tctaatacaa
    31261 atattactat tcaagattac aaaaaagtat atgataatcc tatttcttgc gctctttcac
    31321 aaggtcaaat cgatggacta gcagtcatag aagatggaga gatggtaaat gtttcaactg
    31381 acacatataa attatttgtt catcctaaga ttaaactaaa aaaaggagat cgaataacaa
    31441 taactcaaaa ggccagtggc ttaatttttt ctctatttgc tactaagcct ttttactatc
    31501 ctagtcattg tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa
    31561 tcaaatgcag aaaagatcat tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa
    31621 ggaatatcct tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa accattaact
    31681 cctgtaaaat caggtaattt aaggcgagga tatcgtgtag taaaagctag aaaactatca
    31741 agcggtcgaa ttgttggaga agttataaac aatgaacatt attttaaata tgtagaagaa
    31801 ggtcatagga ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg
    31861 aatcttgcaa atatgtctta tattcctcga agatttaaac aaatggcaat aaaaatcgtt
    31921 aagaaaggaa agtaacatga aagatgaaat cattgctgca atcagtagca aattaaaaga
    31981 aatctatcct gatgggacaa tatatcttga ttcagttatg cagtcaacta aagattttta
    32041 tttcgtttta tcagtaatgg aatctggaac tgaaaatgta ggaattgata ttcaaaatgt
    32101 ttctttctta attgatattg cattgattga taataaccct aatagaaatt taattaatga
    32161 attagtctca cgttgtggga ctttttttaa tacgattaca atcgatgagc aaatattatt
    32221 tccaaaagcc tatttacctg atgaagcaga tggtgttcaa catattcgtt ttacattaga
    32281 atttccacaa tatattgaat ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg
    32341 aattatttct gtcgaaaaac ctacttggtt tcctttggaa gatgagacag ggaattttcc
    32401 ggtatatggt actgcgttgc caatgggaac agcagtaagt atcaaaccaa cagctaatta
    32461 tgaaacaaca caagattatg gggattcagt ggttcaagat caatttacag cttttggtgg
    32521 tgcagaagtt gaactggaag caaatggata tactcataaa gttttatcta caattactgg
    32581 agggaaaatt gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc aagatggtgc
    32641 gtttgcatat agacgtaaaa aatcgaatgg gaaataccgt tatacggtct tttataaagg
    32701 acagtttgct ctagattcag atgaaacatc aacgattgaa ggaagtaaag taagttttac
    32761 tcatccaact tggaagggtt catttgtaga tgtaccagga cttggatata tgtattcagt
    32821 tgatgaagat gatgaaggcg tggataaagc aatgatcgga aattggttta caaaagtagc
    32881 aattccaatt gaagaagcag aactttcagg aggtacagaa taatgtcaaa atatcaaaca
    32941 acgattaaat taacgaaaaa agacgaagaa ggtaaatatg agcaagtaca atttaaatct
    33001 gctgaatttt taccaggaac agttgtagaa gatgctgcag gagttatgga agaaatgcaa
    33061 acagcgactg ataagcaatc agttaaaaaa gctttaagtc gtgcttattc atttattgca
    33121 gatactttat ttgagggaca atttactggt gaagattatt gcaaaggaat tgatgctcgt
    33181 gagattgcct cattgacagg aaaattattg aagtctgtta ctgcaggttt tgatgaaact
    33241 tatacagaga cgaaaaaaaa gtaagtgagg cgctcaagtc accttcattt aagtattcga
    33301 ttacttaccg agaattagat ataaagacgc aattactcga agcaggttgg acgttaccag
    33361 aaattgaacg tactgacttg gatgagttaa tgcgtctttt tgcttttaga gatgcggtaa
    33421 aagagcatga agatgttgag tactatgata atttcactca attttaggag gtgatacttt
    33481 gaataacgat gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat
    33541 gaataatgct gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa
    33601 tagtggcagt tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg
    33661 agtaactaga atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt
    33721 tgatcgagta gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa
    33781 ttcagaaatt atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact
    33841 tcctactgct ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga
    33901 tttaactcgt tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg
    33961 ttcaactgat caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa
    34021 agtagacgca caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc
    34081 tattgctaaa aagatgggaa ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa
    34141 gatttctgtt gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg
    34201 acttaaatca ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca
    34261 aaatgcaaaa acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc
    34321 attagtagat tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat
    34381 ggaatcgttc ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa
    34441 tttatttaac gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt
    34501 tcctgcagct accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat
    34561 aaaaagcttc aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat
    34621 gggggcaata gctgctgcta atccatttgt tttaattatt ggtgcagtcg ttggtgcaat
    34681 agctgttttc ggttacttta tggcaaccaa tgaagagctt agaaacaagg ttatatctat
    34741 ttggaatgat gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat
    34801 tgatacttgg aattctgcta aagaaatggc atcaaatgca gttgagggtg tcaaagatgc
    34861 ttggtcagga acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac
    34921 aggtttattt gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc
    34981 atggtctaat atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag
    35041 agaaaaatgg aatggaatca aaggttctat tatgtcagtt gctggtccat tgattacagg
    35101 tataaagaat gcatttttac atgttacttt ttatctagag accttatgga ataatttagt
    35161 tgagattggt aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt
    35221 tattacctcc atgatttctg gtggatggga agaaacaaaa aataatatga ttgctgtttg
    35281 gaataatatt aaagaaagtg ccataa
//
LOCUS       DASJRQ010000017        34892 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJRQ010000017 DASJRQ010000000
VERSION     DASJRQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.017"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            175..627
                     /locus_tag="U4A80_000798"
     CDS             175..627
                     /locus_tag="U4A80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974433.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(751..1602)
                     /locus_tag="U4A80_000799"
     CDS             complement(751..1602)
                     /locus_tag="U4A80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1974434.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(1615..2400)
                     /locus_tag="U4A80_000800"
     CDS             complement(1615..2400)
                     /locus_tag="U4A80_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1974435.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(2752..3693)
                     /gene="ribF"
                     /locus_tag="U4A80_000801"
     CDS             complement(2752..3693)
                     /gene="ribF"
                     /locus_tag="U4A80_000801"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1974436.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(3697..4620)
                     /gene="truB"
                     /locus_tag="U4A80_000802"
     CDS             complement(3697..4620)
                     /gene="truB"
                     /locus_tag="U4A80_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN1974437.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(4988..7350)
                     /locus_tag="U4A80_000803"
                     /pseudo
     CDS             complement(4988..7350)
                     /locus_tag="U4A80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            7798..7971
                     /locus_tag="U4A80_000804"
     CDS             7798..7971
                     /locus_tag="U4A80_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1974438.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(8128..8475)
                     /gene="rbfA"
                     /locus_tag="U4A80_000805"
     CDS             complement(8128..8475)
                     /gene="rbfA"
                     /locus_tag="U4A80_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1974439.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(8502..10808)
                     /gene="infB"
                     /locus_tag="U4A80_000806"
     CDS             complement(8502..10808)
                     /gene="infB"
                     /locus_tag="U4A80_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1974440.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(10821..11132)
                     /locus_tag="U4A80_000807"
     CDS             complement(10821..11132)
                     /locus_tag="U4A80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1974441.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(11129..11422)
                     /locus_tag="U4A80_000808"
     CDS             complement(11129..11422)
                     /locus_tag="U4A80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1974442.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(11444..12619)
                     /gene="nusA"
                     /locus_tag="U4A80_000809"
     CDS             complement(11444..12619)
                     /gene="nusA"
                     /locus_tag="U4A80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1974443.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(12642..13115)
                     /gene="rimP"
                     /locus_tag="U4A80_000810"
     CDS             complement(12642..13115)
                     /gene="rimP"
                     /locus_tag="U4A80_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1974444.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(13260..17618)
                     /locus_tag="U4A80_000811"
     CDS             complement(13260..17618)
                     /locus_tag="U4A80_000811"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1974445.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(17830..19539)
                     /locus_tag="U4A80_000812"
     CDS             complement(17830..19539)
                     /locus_tag="U4A80_000812"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1974446.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(19607..20875)
                     /gene="rseP"
                     /locus_tag="U4A80_000813"
     CDS             complement(19607..20875)
                     /gene="rseP"
                     /locus_tag="U4A80_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1974447.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(21036..21836)
                     /locus_tag="U4A80_000814"
     CDS             complement(21036..21836)
                     /locus_tag="U4A80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1974448.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(21833..22645)
                     /locus_tag="U4A80_000815"
     CDS             complement(21833..22645)
                     /locus_tag="U4A80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1974449.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(22840..23397)
                     /gene="frr"
                     /locus_tag="U4A80_000816"
     CDS             complement(22840..23397)
                     /gene="frr"
                     /locus_tag="U4A80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1974450.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(23400..24122)
                     /locus_tag="U4A80_000817"
     CDS             complement(23400..24122)
                     /locus_tag="U4A80_000817"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1974451.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(24258..25139)
                     /locus_tag="U4A80_000818"
     CDS             complement(24258..25139)
                     /locus_tag="U4A80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1974452.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(25238..26020)
                     /gene="rpsB"
                     /locus_tag="U4A80_000819"
     CDS             complement(25238..26020)
                     /gene="rpsB"
                     /locus_tag="U4A80_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1974453.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            26379..26858
                     /locus_tag="U4A80_000820"
     CDS             26379..26858
                     /locus_tag="U4A80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1974454.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            27075..28166
                     /gene="phnW"
                     /locus_tag="U4A80_000821"
     CDS             27075..28166
                     /gene="phnW"
                     /locus_tag="U4A80_000821"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1974455.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            28159..28287
                     /locus_tag="U4A80_000822"
     CDS             28159..28287
                     /locus_tag="U4A80_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1974456.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            28291..28836
                     /locus_tag="U4A80_000823"
     CDS             28291..28836
                     /locus_tag="U4A80_000823"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1974457.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(29290..30981)
                     /locus_tag="U4A80_000824"
     CDS             complement(29290..30981)
                     /locus_tag="U4A80_000824"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1974458.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(31401..32348)
                     /gene="arcC"
                     /locus_tag="U4A80_000825"
     CDS             complement(31401..32348)
                     /gene="arcC"
                     /locus_tag="U4A80_000825"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1974459.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(32463..33482)
                     /gene="argF"
                     /locus_tag="U4A80_000826"
     CDS             complement(32463..33482)
                     /gene="argF"
                     /locus_tag="U4A80_000826"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1974460.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(33573..34802)
                     /gene="arcA"
                     /locus_tag="U4A80_000827"
     CDS             complement(33573..34802)
                     /gene="arcA"
                     /locus_tag="U4A80_000827"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1974461.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         9412 a         7485 c         6164 g        11831 t
ORIGIN      
        1 tatatttaaa tataatatca ctttaggaga gaacgaattt ctttagagaa ataaaagtta
       61 gaattttctg acttgatttt tacaatgacc ttaattaaaa tcgttctttc tacatttcgt
      121 gattaagcaa atatcaacta agtcagtaga accattcaca taggagggtg aacattgaaa
      181 gatttttact cagtaaatga attagccgaa caattaggtg ttactacacg ttctatcaga
      241 aattatcttc atgaaggaaa actaaaagga acaaaggttg gcgggcaatg gaaattttct
      301 gaacgtaatc tttttgagtt cctatacgga gatcaagcag aggaagcagc aaaagaaatg
      361 cagcgattca tgctaaatgc accaatcacg atgcgtttca accttcaata tcgtgatttt
      421 acagcaatca atcaattccg agaacaatta gtccagtacc ataacgatgt ttacgcaaat
      481 aaaaaagacc gtcttttaca atacgatctt tataaagaca atcatgcgga gatactaatc
      541 ggtggtaact tcaattatgt cgtaaacttt agtcaatgga tcaatgaaaa attattgatc
      601 cagacagata tttcccttgt atcttaaaca attaaaaagc gctagaaatt ccagagatgt
      661 tggaaattct agcgctattt ttttataagt acttactgtt tggttcaaac aaatattttt
      721 ttaataaatg aaattttacc atctttatcc tcaaataaag accgtgacca tgcttcctac
      781 tacgatcagc aataatccga taatcgttaa ttttttttct tttggtgtct tattttcatg
      841 caggatgtac ataccgccta atgtagagat aactacgttt agctgtgtaa tgatatatgc
      901 agtagcaacc ccattttcat ttgctgaaaa aatataagcc aatgctgaca cactaaagat
      961 ggctccaacg atcgttgctt tccaactctt gtcttcagaa aatgcggttg gtttacggaa
     1021 aatcacataa atcactgccc ctaaaaagac accgatcatt tgcggaaaga aaatggaaag
     1081 tccgcttgca tcaactagtt taggcaatgc actgtatacc caataaccaa tacttgttga
     1141 tgctaataga ctaattcctt ttgtgatacc gccttttttt ccaggatcat cactgattgc
     1201 cgttaaagca gaaccaaata caagaagaat gattgctgct gctccaatga ttttccccat
     1261 acttccagac cattctccaa aagcgaagac accaatcaac gaggttccga ctaactgtaa
     1321 tgcagtcgaa attggcatcg ttttagaaac accaatcaag ttgaaagatg tatactgtcc
     1381 tgtttgtcca ataacccaaa atgcaccaga aagtaaacta atcaaaaatg tagtcactga
     1441 aataccagct ggagaagcga ataattgaac gagtaagccg acaagcaccg ctccaatacc
     1501 agttcctaat atctcatttg ttggtctgcc accaatcttt ttcaaaatca gtggctggat
     1561 cccccagcca actgccggga tcaatgcaat caataaaccc atttttattc acccttatcc
     1621 ttttccacct tggaaagaag gatataaagt catcccacca tctgcaaata gtgtaatacc
     1681 tgtaacataa ctggattcgt ctgaagccaa ccatgcagct gtcgccgcta tttcttttgg
     1741 ttcacctacg cgtcctagtg ggatcatact ttccaagagt tctttttgtt caggatcatc
     1801 aaattttgcc gcattaatag gtgtacggat agcaccaggg cctatactat tgattctgat
     1861 tcccttcggt gcatactcca ttgctaccgt ttcggtaaat aattttaccc cgccttttga
     1921 agcagcgtaa tgagcaaacg ttggccatgg gatctgttca tgaacagaag acatattgat
     1981 gatatttcct tttttattat tctctgtaaa gtaatttaaa gccgctttcg ttccgataaa
     2041 aacgccagtc aaattgactt ttaacactct ttcccaatct tcaaggctaa gttcatgggt
     2101 tggatgctga ttttccatcc cagcattatt aacccaaacg tccagtgtcc caaaattttc
     2161 taaagccgta tcaagtaaac gctgtattcc ttcttcagaa ctgacatctg cttgtacaga
     2221 gactgctttt ccgcctgcct cttcgattgc tttgactgct ttttctgctc cttctttatc
     2281 cgagttatag ttgacaacta cattcatttt ttctttcccg aatcgttgag caatcgctgt
     2341 tccaatcccc tttgagccac cagttactac tgcgactttg ttttctaatt ccttgtacat
     2401 agtacccact cctttttcat acctaaacct gttattacgg ataactttta tttccgcctt
     2461 aaagtgagtg tacaccaaga tatatagtct aaagcaaatg atacgctcta gcaatcgttt
     2521 tcatgaattg ctagtgtcct tttatttcta taaagaaaga ggttgtgaca gaagtggtca
     2581 gcttcaagaa atttcggctt atttccgaag aagctacttc tgaaaaacca tttatttgga
     2641 aaaaaaggct gtgacaagac ttttgtcaca gcccctttcc tgggcttatt tatcacccat
     2701 tttattgtaa ttgctagtta tacataaaca agagatttta tgcataatgt tttattgctg
     2761 agaaaaatat tcagctgttg cttgttcatc ctccttgagt tgttcaatca attcctctac
     2821 actagaaaat ttcacttgat ctcttaaaaa ttgattccag cgtaccgcga catgttcacc
     2881 atagatatct tgattaaagt ccaaaatata caactctaca gtcagctccc gtccttcacc
     2941 gaatgtatca ttatgtccaa ttgaacccat ggctggatac catgtttccc caatttttat
     3001 ttccgacaca taaacacctt ctttaggcaa atgaaccgtg ctctttactt tgacatttgc
     3061 tgtagggaat cctagtagtc tgcctcgtgc atcgccatgg acaactaccc cttctacctc
     3121 atatgtgcaa cctaatagtt ttgctgcttc ttttacttgt ccgctgtcta gtaatccacg
     3181 aatacgggta gagctgattt tttcaccttg ttcttcttct ttagaaactg tgacgatttt
     3241 gaatcttcct ttggcatatt caggaaaatg agcgacatct gcaatgtctt ttggtccata
     3301 cgtataatca aaaccggaaa cagcatattt tgcatgtaac cctacgatgt attgatcaac
     3361 aaattcctgc ggtgctaaat gagcaaaaga agaagtaaaa tcaacttcat ataaataatc
     3421 tatacccaaa gcggccattt tttcttcttt ttgcttaagg ctagtcaaat atttcacgct
     3481 gctaggatcg atctttttaa agacaataga tggatgctga ttgaaagtca tcaatgctaa
     3541 tttcaatcct tctttttgtg caatttccat acctgtcttt atgactttct ggtgtccgat
     3601 atgcacgccg tcgaaaaatc ccagcactaa aacgacatcg tcttctggaa tttgttctga
     3661 ttggtaagga tgtctgatct tgatgatatt cattcgttat tcctcatttc tcaatacttt
     3721 actaggtttt aatttgtttt tttctttcgg gtttggctga taaatactga caacttttcc
     3781 tttataaaac aaagcaattt cttctgacgg catttctggg agcgcaaata cagtataatc
     3841 aagtctcata ccgtttttta ctttctgcca tacttcatca gtaatatcta ctcgtttgaa
     3901 ccgttccact cctttttcta ttggcagcaa acaatcatcg attgtcccat ttttcacagc
     3961 ttgagctact tcttctaatg tcagagcatc cttctgggac ataccggcac ttgctgttcg
     4021 ggtcagatca gacatgtgcg cagggtaacc aagtttcttg cctgtatcta cagctaatgt
     4081 gcggacatag gttcctttcc cgcattctac ttcgaagcgc caagaaatgg ttcctgtcac
     4141 ttcgtctctt gtcaattcac ttgtccggat aaaacggtaa atctgagcct ttctaacagg
     4201 acgctccact gtttcattat tgcgggcata ttcatataac cgcttaccgt tgacttttac
     4261 cgctgagtac attggtggta tctgggtgat ctcaccaata aatgaagcca tcacttcatc
     4321 tatttttttt tcagataaag acatatctac tggttttgtt tctacccgtt ctccggatcg
     4381 atcttccgtc gttgtactga aacctagagt tatctcgcct acatagattt tcccagaatc
     4441 tgtcaaatat tcgataactt ttgtggcttt tccaatacaa atgggcaaca caccgtctac
     4501 gtctgggtca agcgtacccc catgtccgat ttttttcgta tgtaagattt ttcttaattt
     4561 gaatacgcaa tcatgacttg tcattccgcg ttctttccat aaaggtaata acccgtccat
     4621 gtcttttctc catttcattt ttgatttaac tgaattatta taacacattg catcaattac
     4681 tgacgaattt gacatcataa tgaacggttt ttatttttcg gcaataaaaa cgtaacaaaa
     4741 ttcatgctca cgtaaataga aatcatgtta tgctttttta aaggagggaa tagaatggaa
     4801 gattttttct ttgcaccatt tgattggatt tatgggtggc ttacaggtaa atagtttggt
     4861 tttcacttcc caaaaacgaa aaaaaagact ggccattctt ccagtctttt ttccgttttt
     4921 tttatttatt ttttcagttt cttaatcaat gtaaaaaatt ctattttgcg ggatcgattt
     4981 tatcatttta ttggtcaaga tttttccatt tccaatccaa tacttctggc agatcaatac
     5041 cattttcacg aatatagttt tgatgttctt gtaatttctg atccattttt ttcgcaaaat
     5101 cttctgcttg acttcctttg atttttaaag ctgcgtcttt tgccaaatga taacgatcaa
     5161 gttcatttac tacacgcatg tcaaatggtg tcgtgatatc tccattttct ttgtagccat
     5221 gaatcattaa atgatggtta tgacgatcaa agaaaatcgt gcggaccaat gtttcataac
     5281 cgtggaaagc aaataagatc ggtttgtctt ttgtaaaata ttgatcaaat tcttcatcgc
     5341 tcaacccacg cggatcttga cttggatgac gtaatttcaa taaatctacg acattgatga
     5401 accgaatttt tagttttggg aattcttcca gcagcaaagt tacagctgct aatgcttcta
     5461 agttagattc cgttcctgca gcagcaatga ccaattcagg ttcctcctct tttgctgtcg
     5521 aagcccaatc aatgattttc aagccttcat tcactaagac ggctgcttct tctgctgagt
     5581 aaaattgagc acgcggatgt ttggaagaga tgatcaaatt gattttttct gacgagcgga
     5641 atgctttatc catgactgcg agcaatgtat ttgcatccgc aggcagatat tcacggatga
     5701 actccgcctt cttttctgcc aaatgtgtca atattcctgg atcttggtgg ctatatccat
     5761 tgtgatcttg ctggaagact gttgagcttg caatgagatt tagcgaagga taatcatttc
     5821 gccaagaaag gtcatgtgat ttccgcatcc atttgaagtg ctgagtcaac atagagtcaa
     5881 ccactcgcaa gaatgattcg taacttgcaa aaaaccatgc cgtccagtca gaacgtaccc
     5941 ttctaagaac ccttcatctt ggtgttccga taattgactg tcgatgacgc gaccagatgg
     6001 cgaaagccat tcatcatttt ctggttcgat tttttccatc cattggcgat ttgtcgtttt
     6061 gaaaacttga ttcaatcggt ttgattttgt ttcatctggt ccaaatatac ggaaattttc
     6121 tggatttttc tcaatgatct cgcgaacgaa tttaccaagt tcggtcatat cttctgcttt
     6181 aacggagcct ggtttttcaa attgcaaagt atattttttc caatctggca taataagcgg
     6241 tcttggatcg attccaccat tggtgatagg attttttgcc attctttggt caccagtagg
     6301 cgcgatttct ctgatttctt ctaaaatacg tccttgtgca tcaaataatt tttctggttc
     6361 ataggatttc agccaacgga gtaaagcatc tgcatgatcc atatgttctt gatctacagg
     6421 aatcggtact tggtgtgctc ggaaggttcc ttcaattggt tccccgtccc attcttttgg
     6481 accggtccat ccttttggtg tgcgtacgat caatactggc cattttggca tcacgacatc
     6541 tgttgctgtc ttcaggcgag cttctttttg gatggattta attttctcga ttgcttgatc
     6601 cattttttca gccataacag gatttaattt ttctgaatca tttccttcaa taaagaaagg
     6661 ttcccagcca agtccgttaa agtaatttgc taattctcca tctgatttac gtgccaagat
     6721 cgttgggttg gctatttttg caccatttaa atgaaggatc ggtaaaacag ctccatctat
     6781 cactggattg ataaagacat ttgagaacca gcttccagct aaaggaccgg tttctgcttc
     6841 accatcacca atcacgacag cagagattac ttcaggattg tctaatacag ctccgactgc
     6901 atgtgaaaga gagtatccaa gctctccgcc ttcatggata gaacctggtg tttctggtgc
     6961 tgcatgagaa gcaatgcctc caggaaatga aaatcgtttg aataattttt gcattcctgt
     7021 ctcatcttcc gttacttctg gatagatctc tgtgtaactt ccatctaaat aagcattcga
     7081 taccatgact tgtccgccgt gtcctggccc ttcaatataa aacatgttca aatcatattt
     7141 attgatgact cggttcaaat ggacataaat aaaattttgg ccaggaatcg ttccccaatg
     7201 accgattgga tgttttttga catcttcggg tttcagtgta cgtctcagta aggggttatc
     7261 tttaagataa agttgcccaa cagaaagata attagcagca cgccaccaag ccgtcatttt
     7321 atcaaagtat tctttactag aataatccat acataacact ccttttcatt attactctta
     7381 ttctagcgca gatactccca attgaaacct aataagcact tctgttcatt tgtttggatt
     7441 ttatatctag ccaaatgaac aggtataaaa aaccacgaaa tttttccttt cgctgacgtt
     7501 ttttacattg atccaaaaaa cttgtcgtta tcacaatggc tgttccgcag ttcatttcgc
     7561 tcctcatcat gcataatttg ctgaatgacg ctgggccagt taacacattg ctggaaaact
     7621 ggggctggat cgagcaattc ctttcctgat tgatccgttt tatgtaaaag tctctgttat
     7681 ctttgtcaat atgtgaattg gtatcccagc tactatgcta atttctacag ggattatcca
     7741 aaatttggat cgtgaccaaa tcgaagcggc gtaatcggaa atatcaataa cttcaacgtg
     7801 atttttcttt taactgtcaa cggaccgaca aactcaaact tttatggtgc aggttccact
     7861 gatcttctag ttacttggct atacctgact gtcactaaaa tagattacaa tctagcttcc
     7921 gttatcggta tcttgatctt tatctatatg caaaaattta tatactacta gaaaaaagaa
     7981 agtcgaattt tttacttttc tttttctctt ttttggttag ctgacacgcc gcaagactaa
     8041 agtcatggaa ctcttaggta atacgtctta atgaaaacaa aaaaatcccg caacccctga
     8101 tttcacagga tttgcgggag tttttgatta gtctttgttc aagtcgcgaa gcaattcatc
     8161 gatatggttg ccatagacaa ctgattcatc aagttcaaag aacaattcag gtgtcttata
     8221 aatacttaga ttgtgcccta actctcgacg aatcaatcct gatgctttat tcaatccatc
     8281 ttgtgctgtt ttcttatctg atgctaagtc agataaaatg ctgtaataga tcgttgcttg
     8341 ctgtaagtcc cccgtaactt tcacatctgt gatcgttacg ttctccacac gagggtcacg
     8401 gacctttttg cgcaaaatat cattgacttc ttttaagatt tcttgaccga ctcggcggtc
     8461 acggtagtta gccataatga ttggcctcct tttatcagtt tttagtcatt tttgacttct
     8521 tccatgataa atccttcaat tacgtcatct actttgatgt cattgaattt ttcaatcatt
     8581 gcaccacatt cataacccat tttgacttct ttcacgtcat ctttgaatcg tttcagactt
     8641 gcaagttggc cttcatagat tacgattccg tcacggatta cacggacacc gctatctcta
     8701 cgaatatatc cttctgttac ataggcacct gcaatcgtac caactttaga aactttgaag
     8761 gtttcacgaa cgatcatttg accggtgatt ttttcttcaa attcagggtc tagcatccct
     8821 ttcatcgcag tttcgatttc ttcgatggct ttatagatga tacggtgaag acgaatatcg
     8881 acttcttcag tatcagcttg cacttttgcc tgcggtgtag gacgaacgtt gaaaccgatg
     8941 ataatagcgt tgctggctgc tgctaaggtt acatcacttt cattgattgc tccgacagct
     9001 gaatggacga tttttacacg gacaccttcc acatcaatct tcttcaatga tgcagccagt
     9061 gcttctgctg aaccttgtac atctgctttg atgattacgt taacttcttt caattcgcct
     9121 tctttcaggc tttcaaataa gttatccaaa gtcacacggc ttgtggcagc acggttttct
     9181 aataatgcac gtttcgcgcg ttcttcaccc gctgcacgtg ctgtcttttc gtcttcgaag
     9241 actacgaaac ggtcacctgc ttgaggtaca tcattcaagc cagtgatttc tactggcgtt
     9301 gcaggtcctg cttctttatc gcgacggcca agatcgtttg tcattacacg gacacgtccg
     9361 taagtattac cgacaacgat tgggtcacca acatgaagtg ttccttcttg gacaagtaag
     9421 gttgacacag gacctttacc tttatcaaga cgtgcttcga tcactgttcc gattgcgcgt
     9481 tgtgttggat ctgcttttaa atcttctact tctgcgacta acaggatcat ttccagcaat
     9541 tcttcgatgt tttggccgaa tttcgctgag atctcaacaa agatcgtttc tccaccccat
     9601 gcttcaggaa ttaactcata ctcacttaat tcttgcataa catgttgagg gtttgcacct
     9661 ggtttatcaa ttttatttac agcgacgatg attggaacgc ctgcagcttt ggcatggttg
     9721 attgcttcaa ctgtttgtgg catgacgcca tcatctgcag caacaactaa gatcgtgata
     9781 tctgtgatac ttgctccacg tgcacgcata cttgtaaagg ccgcatgtcc tggtgtatcc
     9841 aagaaagtga ttggtttacc atcaatatcg atctggtagg caccgatatg ctgtgtgatt
     9901 ccgccggctt cgccgcttgt tacacgtgaa tggcgtaatg tatccaataa agttgttttc
     9961 ccatggtcta cgtgtcccat aatcgttacg actggcggac gtgagactaa tttatctgga
    10021 tcgacttctt ctgcctcgaa gaatttatcg atatcagcaa tgtcaacttg gactttttct
    10081 tgtggttcca tgccatagtc cgtcgctaac agttcaatcg tatctttatc cagtgcttgg
    10141 ttttgattga ccatgacacc aagcataaat aattttttaa tgatttctgc tggctcacga
    10201 tgaatttttt tcgcaatatc tgctacgttc ataccttcag tatactctaa tacatctggc
    10261 aattcacgga atttgcgtgg tggtacagct ggtttagttg attgttgctg tttgcctttt
    10321 ttaccttttt tattataacg attacggtta ttattattaa atttgccgcg attgttattg
    10381 tttggtttgt tgttattgtt agtacggttt tgattgccgc cttggctcgt tccttgacga
    10441 ttttggctgc tggtatttcc cgtattattt tggtttgttg ttggtttttt tccttgattc
    10501 acttgaccgc tcccttgacc gcgatcttga taactgcggt tatttttatt tttttgttga
    10561 ttgttcgttg tttctttttt acgttgatcg ttcggctgtt gattggctgg ttttctgctt
    10621 gcttgttgtg cagcttgttg tttcgtgctt gggtttttga acgcttgttg caatttcttc
    10681 tcatcattgc tagtaatcgc acccatatgg tttttcacat cgatgccaag ttgttgtgct
    10741 ttttccacga cgtctttact agattttttg agttcttttg ctaattcata gattcttttg
    10801 ttgcccatgc aatcaccttc ctaaccttca attagctcct tggtcttctt cgcaaatccg
    10861 gcatccatta ttccgaatac cttgcgaggt tttccgatca tctgcgtgat ttctgcttca
    10921 gaaaatagct caaagcaagg aacttcataa tatgaactct tatcttttat cttttttctt
    10981 gtattctcgc ttgcatcggt tgccacaaac accaattttg ctttttgttt gcggatttca
    11041 gcaatggtca gttcttcacc cgtgattagt tttcccgcgc gcatcgctag accgatgaga
    11101 ttcatggctt tttgccgatt aactgtattc attttccaaa taactcttta cgtgcttttt
    11161 ggtgagtgac atagtcaaga agttcttgat aaaactcatc tgttaatgtt gtttcgagta
    11221 ctcgatcgag aattttttta tcccaagctt gttgtacttc ttccggctca agtgagacat
    11281 acgctcctcg tccaggcatt tttccagttg gatcgatcga tacttcccct tcttttgaac
    11341 gggtgattcg gaccatctct tttttaggtt tcatttcacc tgaaaccacg gatttgcgta
    11401 aaggaatttt cctttgtttc atctctttgc ctccttacta aagttaagct tctggttttt
    11461 cttcgaccgt ttcattgttg aatgcaattg tttcatattc atcgtctgtt aaatctgact
    11521 gaacgattgc ttcatcatgc agttcttctg tttctttttg ttgttcttga ttttcataaa
    11581 attccgtcat gtcagattct gatttgatat cgattttatg gttagtcaat ttagcagcta
    11641 aacgtgcgtt ttgtccgcgt ttaccgattg ctagcgataa ttggtagtct ggaacgacaa
    11701 ctgtgcaggc ctttggattt gtctcatcga agatcacatc catgacttgt gcaggattca
    11761 atgcatttgc aatgaataca gctggatctt cattccattc gacgatatcc atgttttcgc
    11821 cttttaattc attgacgatt gcttgaactc gttgtccctt tggcccgaca catgtaccga
    11881 ctgcatcgat atttggatca tttgagcgaa ctgccacttt tgaacgatcg ccagcttcac
    11941 gggcgatgct gacaatttca acgattccat catagacttc tggtacttct tgctcaaata
    12001 agcggcgcaa taggtctgga tggctgcggc taacaaatac ttgcggacct ttagacgtat
    12061 tttctactcg ggaaacataa actttgattc gatcatgtgg ttgataaaat tcatttggca
    12121 tttgatcttg tttagaaaga actgcctcaa ttttaccaag attgacataa atataacgtt
    12181 tatcctggcg ttcgacgatt ccttgcatga tgtctttttc ataagcactg aattcgttat
    12241 aaataatcgt tctttctgct tcacgtacac gctgtaaaat aacttgttta gccgtttgcg
    12301 cagcgatccg cccaaaatct tttggtgtta cttcaaaacg gatcgtatca ccgatctcat
    12361 atgctggatt gattaaaagt gcatctttca atgagacttc caactgtgaa tccatcactt
    12421 cttccgttac ttcttttacc gcatagacat ggatctttcc ttttttctga tcaaattcca
    12481 cttcgacgtt ttgtgcttgg ccatagtgac gtttgtatgc agaaaccaat gctgcttcta
    12541 aagcatcgat cacgatttct tttgagatcc ctttttcagc ttctaaagcg tctaacgcat
    12601 ttaacatttc tttactcatt ttttctgatt cctctccgtg attaaaattg gatagctaag
    12661 cgagcttttg caatattttt tcggtcaaaa gtcaattctt tttctcgtgt ttttatgcgt
    12721 attttcaatg taagctgttc agcatcaaat gattgaagaa atccttcata ttgtttttcg
    12781 ccatctactg gttggtataa ggagacatgg acataatcac caagtgcctt ttcatagtcg
    12841 ctttcttttt tcaatggccg ctctgctccc ggggaggata cctccaggaa ataagcttga
    12901 ggaatcggat ctggatctgc tgcatcaagt ttttcactca gtttctcact gacaaaggcg
    12961 cattcttcga tatcgatacc gccttctttg tcaataaata ctcgtaagaa ccagcttttt
    13021 ccttctttta caaattcgac ttcgacaagt tcaaaatttt gttcatctaa aatcggtgtc
    13081 accatttctg tgactgtttc aacgacgcta ctcaaaagtt tcgcctcctt aaaaaattaa
    13141 gtgcgtaatt ccattaaaaa gagtgagcga aaaatccgct cactcacagt tagtatcatt
    13201 acctataaga actataccat actctgctta aaattacaag cagacaggaa aacagatgct
    13261 tatagcatat cgaataacga taattgattt tcatctggta gatctttcaa tacgccattg
    13321 tcgttcatat attcgatcaa tgtttttgaa actttaccgc gggtggcaag atcttctttg
    13381 gataaaaatg gaccgtcttt tcttgcttca acgatttgtt ttgccacgtt ggttcccaaa
    13441 cttgggacag ctctaaatgg tgcgatcaac gtatcacctt caataacaaa gttaacggca
    13501 tccgatttat acaaatcaat cattccaaat ttgaatcctc gttccagcat ttcattggct
    13561 aactccagca ctgtcagttg attcttttct tttacggaag catctaatcc tttgtccgtg
    13621 atttctttca ttgcttgttt cacagcatct tttcctttgc acatggagac tagatcaaag
    13681 tcgtctgcac gtacagaaaa gtatgcacag taatacaaaa tagggaaata aaccttgaaa
    13741 taagcgacac gaagagccat caatacgtaa gctgcagcat gggctttcgg aaacatatat
    13801 ttgatttttg aacaagaatc gatataccag tcgggcacat tattttcttt catcgccgat
    13861 agataggttt ctcgcaattc atcagggatc ttgttccatt gtcctttacg aacagtttcc
    13921 atgattttaa atgccatccc gctatctaat ccagcatgga tcaaataaac cataatatca
    13981 tcacgacagc cgattacttc cgccagtgtc gcatctcctc gtcgaatcaa ttcttccgca
    14041 tttcctaacc atacgtctgt accatgagat agtccggaaa tttgcagtag ttctgcaaaa
    14101 gttgtaggat gcgtttcttc tagcatccct cgaacaaaac gagtgccgaa ttctggaata
    14161 cctaatgtcc ctgttttgga ataaatttgt tcctgactta ctccaagtac ttcaggaccg
    14221 gcaaatatcc gcatcacttc cgggtcatcc gtagggatcg tttgtggatc gatccctgat
    14281 aaatcttgaa gcatccgaat aacagttggg tcatcatgcc ctagaatatc taatttcaat
    14341 acattatcgt gaatcgagtg aaaatcaaaa tgagtcgttt tccattccga attttgatcg
    14401 tctgctggat attggatcgg cgtaaaatca tagacatcca tataatcggg aataacgatg
    14461 atcccccctg gatgctggcc ggttgtccgt ttgactccag tagcgccttt tgctaatctg
    14521 tcgacttctg cacttcgaaa ctgcagatta ttgtcccttt cgtatccttt tacatagcca
    14581 tatgctgttt tgtcggcgac tgtaccaatc gttccggcac ggtaaacata gtcttcgcca
    14641 aacaatactt tcgtatagtt atgggcttct gcttgataat cacctgagaa gttcaaatca
    14701 atatcgggca ctttgtctcc atggaatcct aaaaatgtct caaatggaat atcatgaccg
    14761 tctttgttta agcgtgcacc acattttgga cattgttttt ctggcatgtc aaatccagaa
    14821 ccatacgtac catcttcaaa aaactcggaa tattggcact ctggacaata ataatgaggg
    14881 gctaacggat taacttcagt gattcctgtc atcgtcgcaa caaagctaga gccaacagag
    14941 ccacgtgaac ctactagata gccatcttca ttacttttat gcactagttt ttgggcgatc
    15001 agataaataa ccgagaagcc attaccatta atagaattca actctttttt taatcgtttt
    15061 tgaatgatct caggtaacgg atctccgtac atttgttttg cttttgtgta actcaattct
    15121 gatatttcat cttctgaacc tggaatcttc ggtgtataaa gttcatcttt gactggaatc
    15181 acttcttcgc agatatctgc gattttattc gtattttcta ctacgatctc tttcgctgtt
    15241 tcttccccta aaaagtgaaa tgcagttagc atctcatcgg ttgttcggaa atggacatcc
    15301 ggcaaactat gacgattcaa cggattcgct ccgcccatag aattgatcaa aatttttcgg
    15361 taaatggcat cttcttcgtt cagataatgt acattacctg ttgcaacaac gattttatcc
    15421 aaactttttc cgatttctac caaattttgg ataatttctt ccaaatcatg ttcatttttt
    15481 accaattctt gctcgatcaa aggcgcatag accgcttttg gcataacttc aatataatcg
    15541 tagaattttg ctcggtttct tgcctcttct accccttttt gcatcatggc ttcgaatatt
    15601 tctcccttat cacaagctga gccgatcaat agattttcac gcatcttttt caattgggag
    15661 cgtggtattc gtgggacgcg ttcgaagtat tcgacattcg acattgaaat cagtttgaac
    15721 aaatctttca gtcctgcctg attttttgct aaaatcgtta catggaaagg tcgagccctt
    15781 ttgtaagaat ctccttctcc gatatgctca tttagctgat catgataaag catgttatga
    15841 ttgtccattg cctctttcac aaagatccat gctaagtgac cagttgcttc agcatcatag
    15901 attgctcggt gatgctgttc caagctgaca ccaaattttt tagataacac acctaatccg
    15961 aaacgtttga attgaggata cagatatcta gctaattcca acgtatcaat gactggattg
    16021 gccgcttcag gaataccata ccttgcgtag ctagtattca agaaccccat atcaaaagcc
    16081 gcgttgtggg ctaccaaaat tgtatctttt gaaaattcaa ggaacatccg caatacttct
    16141 tcttccgact ttgatcctcg aaccattcca tccgtgatac cagtcagatc taccgttgtt
    16201 cgtgacaaag gatgtcctgg atcgataaat tcatcaaagc tttcgatcac atttccttta
    16261 tacattttga ctgcggctag ctcgatgatc gtgtcatata ctgcagacaa tcctgtcgtt
    16321 tccacgtcaa atacgacata tgtggcttca ctcaatgctt catgtgcatc attataagca
    16381 attgggacac catcatctac gacattcgct tccacgccgt ataagatttt cacacctgct
    16441 ttttttccag cactatgtgc ttccggaaag gcttgcgcac caccgtggtc tgtaatggcg
    16501 atagctcggt gtccccattt ccctgcttgt gccacaagat cagagatgct gtttgttgca
    16561 tccatcgtac tcatattgct atgaacatga agctctactc gtttttcccc ttcaggagca
    16621 taatctttgc gaggtgtatg tttgacttct atgatatctt gcgcattcat gaccaaatca
    16681 cggacaaacg tatcttcttg aatacttcct cgtaccttta accagctacc gacactgatc
    16741 gcatcaaata cttgttcatc tttttcgccg ttcgagaatt ttttgacgat aaatgatgaa
    16801 gtataatctg tgattttcaa agtcaagatc ttgcgttttg aacgtagttc tctgacttct
    16861 ttgtcgaata cgtacccctc aagtgtgact cttcgttctt cttcaacgat actgatcatt
    16921 ggtgtcgttg gttcatcggc tggaatattt cgtcctagct ggatgtggat gggtccttca
    16981 agagctggcg attgctcttt tctttccttt ttcttttgtt cgtggacaac tagactttca
    17041 gctgcttgct tcataaaggc ttctgcctgt tcttgtttgc gttcttcaaa aagtttcaat
    17101 actcgttccg cttgctgctc atccacttct ggttcgatac gaaattttgg gaatccgtaa
    17161 ctgacgaaca actcttctac taatggcaag tattgttgct tcaaataaga aatagctcct
    17221 gtactgtcga ttggcaagat gactttacga tcttttatca caggtacctg cgttttcaat
    17281 acttgctgtg ctagtggtgt atcgcattgc tgattttcca aagcttgggc ccagtaatct
    17341 tgcaagagct gttcatcgaa tgtctggtca tctgctaaga tattcagttt gacttgtgca
    17401 atatctttaa aagcgatagt cagatgctga gtcaatgacc gatatagcat aataggcaaa
    17461 attttggaaa aatgcagcgt aaattcccat aaacgcgatt tacgatggac cactacacta
    17521 tccatttcac cagcagtaag cagtggatga tttttttctg cttcctctag ttggatttgt
    17581 tccaacagtt tttcaaataa atctcgtttt tctgacaaag atatacgccc tttctgatga
    17641 gaaacaatgc aattgatcgc atcttatcct cttctttttc agtacctttt agtatactcg
    17701 ttttttctga gttttacatt aaaaaattgg aactgcgatt caaatcgcag ttccaatttt
    17761 aaaagcgaat catcagattg tcaaaaacag ctgattcagc tatttttatt ttctgtgttt
    17821 taacgcttat tattcgcctt ggttcaagag gattggtaaa gtattcacca attcttcttt
    17881 acgtacttca accatttcgc cagttttctt gattttcact tctacgattc catccactgc
    17941 ttttttaccg acagtgattc tgattggaca gccaatcaaa tcagaatccg cgaatttcac
    18001 tcctgcgcgt tcatttctgt catcaaccag tacttcatag ccagcttttg tcatcgcttc
    18061 ttctagttca ttcgtcaggt ttgtttggta ttcgtctttt agattcattt ggacgatatg
    18121 caaatcaaat ggcgcaattc cttttggcca gttgattcct tgttcatctg aatgttgttc
    18181 tacgattgct gataataaac gacttacacc gataccataa cagcccataa tcacaaattt
    18241 ttctcgtcca ttttcgtcta gtacagttgc tcccatagat tcactgtaac gagtgccgat
    18301 tttgaagata tgtccgattt cgattcctcg agtaaaggca agaacaccat tgttgtctgg
    18361 tgaaggatct ccttcttgta caaaacgaag atcttcataa gaaagtacag tgaaatcgcg
    18421 ttctggattc acatttttat agtggaatcc tgtttcgttc gctccagtaa tggcattcgc
    18481 taaatcttgg acataacggt ctgcatatac ttttacgtca tctgaaacac cgactggtcc
    18541 gacagaacca aactcagcac ctagatattt ttgtgcttct tcttctgttg cttcttctaa
    18601 aaagtcagca gataaaaagt ttttcagttt tacgtcattg acttcatggt ctccgcgtac
    18661 caaaacaagg acaggttgtt catccgcaat gaacaataca gacttgatga ttttttgagg
    18721 ttcaacagaa aagaaatctg ctacttcgtc aattgatttt acgttaggtg tagctacttt
    18781 ctccaattca agctgtgttt catgcgattt ttttgataca tagtagcttg tagccatctc
    18841 caagtttgca gcataatcac tttcagtcga ataacagatc gtatcctccc cgatttctga
    18901 gatcgccatg aattcttttg agtctttccc gcccattgcg ccaccgtctc cgataatcga
    18961 gcgaaattct aagccacaac gcttgaatac ttcggtgtag gctttttcgt aatctttata
    19021 tgtctcgtcc aaactttctt ccgttgcatg gaaagaatac gcatctttca tgataaactc
    19081 tcttccgcga agcaaaccga agcgaggtcg tttttcgtca cgatatttag gttggatttg
    19141 ataaagattt aaaggtaatt tcttatatga attgatttca ttacgcatca aatcagtaaa
    19201 agtttcttcg tgagttggtc caaggatcat ttttcgatca ttgcggtctt tgaaacgata
    19261 caagtttggt ccatatgttt cgtaacgccc ggattcttcc caaagttctg ctggcaagat
    19321 ggcaggcatc agcatttcta ccgctccgat tttttcaaat tcttcccgca taatgcgttt
    19381 cagtttttcc aatacacgat ttgctaatgg cagatatgaa taaatacccg ctgatacttg
    19441 gcggatatat ccagctctta gcaagatttg atggcttaat acttctgcat catttggaac
    19501 ttcgcgtaat gttggaatca acatttttga ttgtctcatt ctatatactc ctttatttcc
    19561 tcaaaatagt tatcgtttgt tattataccc tatttgtcaa ctttttctag aaaaagaatc
    19621 gttgaatatc gttccatgta actagcacca tcaacaacat taaaaatcca aagccagcta
    19681 atgtcaatat tccttctttt tcttgactta atggtttccc gcgaatccct tcaaaaatat
    19741 tcaagactaa tttcccgcca tctaaggcag gaatcggaag cagattgacg attccgagat
    19801 tcattgaaag aagcgccatc aaactaataa cagttgttac accttgattt gctgcttctg
    19861 aagacaattg gtacatcatg acaggtccgc ctaatttatc taaactgaaa cctgtaaata
    19921 gtgatcctaa ggctttgaaa atttccaatg aaccgctgaa agcttgacgc gtccctccaa
    19981 tgatcttatc cataaaacct gtattcattg gggcttttat accaagttgg ccaacttttt
    20041 cgccatttga ctctacagat ttcggtgtca cgtcgacaga ttgcacctga ccttctcgtt
    20101 cgatcttaaa gtctaatgtt ttatccggat ttttcgtaat gactgttgtc agatcattcc
    20161 atgaatggat ttcttttccg tccacactga ctacctcatc attttctttt aagcctgctt
    20221 ctgccgcagc tccattaggc atgatctctc ctacgcgatt agtgttcgtc acttgaacac
    20281 caccttgcat aaatgccaga atgatgaata acaggattgc caaaataaag ttattcatgg
    20341 gtccagcaaa gttggtcagc atacgctgcc acaatttagc cgattggaac tgtacgtctt
    20401 ttggcgcaat tctgatttct gttccgtcgt tttcaataat cgtcgcatcg tgatcaacag
    20461 ggtaggttac tacttctgtt tcgtctccat tcacataacc agtgatagtc agctcatctt
    20521 ctaaatcata acggctcaat tccatcggga tagcatttgt caattggatt ttcttactta
    20581 aatttatctt ctctacgatg ccgtcagaat ttagtaaaag agaaagaggc attcctggtg
    20641 ccatttctgt ttcatcatcc ccattgcctg ccattcgaac ataaccgcca ataggtaata
    20701 ggcgaagcgt atatgttgtc ccgtcttttg cttgatgccc ataaattttc ggacccatac
    20761 cgatagcaaa ttcgcgaacg aggattcctg atcgtttcgc aaagaagaaa tgaccaaact
    20821 catgaacaat cactaatata ccaaaaacga tgataaatgt cagaatcgtt ttcatagctt
    20881 ttttctcctt tacttgtttt ttgtgttttt tataaaacaa cgataaaaca ctataatttt
    20941 accatagtat accaaagaca ccaatagtat caatataatg aaaaataaaa acctgcgtct
    21001 tttatgaaat tcacaaagaa acgcaggtat acttactaaa taatgccaaa taaatgcatg
    21061 ataggaaata caaacagcaa actgtcgaaa cggtctaaga ttccaccatg gcctggtaag
    21121 atatttcccg agtctttcac atcataatga cgtttgattg cagattcgac caaatcacca
    21181 aattgtccga ctgctgaaaa aataatcgtc atcagaagca tgacaaacat accatgatta
    21241 aatagttctt ttgctgggta tagcaataaa taaagaaatg cgactacaac agcactacca
    21301 ataccaccga gcgcaccttc aatcgttttg ttgggcgaaa tatcaggact taacttatgc
    21361 tgaccataac gtcgaccaac catatatgct ccgatatcag ttgcccaaac aataaataga
    21421 ccaaatatca aaacagccaa gctttctgtt cgtgcattga caaaattctg gaaacctatt
    21481 cccacataca agctgacaag cacaggaaaa cctgcttcat caatcgtata cgtattttta
    21541 gaaataactg atgttcctaa aagaatcata actataagat aaaataacat aaatgtactt
    21601 acattctctg gaagaaagaa aaaccatctt tcttttggca aaacaagtaa cactgcacca
    21661 acggcggata aaactccttc aaaacttaag agtgtcaatc ctttcatccg aaacagctcg
    21721 taaactccta ttactgctaa gattgctgct gtaagctcaa ccgctatgcc tccgatccat
    21781 ataatcggaa taaacaagac tagcgccacg gctgctgtga ccacacgttg tttcatgact
    21841 gatccccttt ctcatcttct gtttttttta caccgccgaa tcgacggtct cgattttgat
    21901 aagaggcgat tgcttcttca agatgcgcac catcaaaatc aggccaaagc gcttttgtaa
    21961 aatacagctc actatatgcg atctgccata acagaaaatt actaattcgc tcttctccac
    22021 ttgtacggat catcagctca ggatcttgta actctttagg caagaaacct gtcattaaat
    22081 gcttagcaat caattcatca tcgatttctt ctgcgtgtat ttctcctttt gctacttctt
    22141 cggcaatttc ttggacagca gtcacgatct ctgctctgct tccataattc aaagcaaaat
    22201 tcaataccat tccagtattg ttttttgttt gctcaattgc acgtcttaca gcatcttgcg
    22261 tatgttctgg caatacgttt tcataaccca tgacatgaac tttgacattt tctttaatca
    22321 attctggtac gaacgtatca aagaagtcga caggtagttg catgagaaaa cttacttcat
    22381 cttttggtcg cttccagttt tcagtcgaaa aggcataaag cgtcaacact ttcactccaa
    22441 gccttgatgc tttttttgtg actttcttta ctgtttccat gccttcttta tggccagcaa
    22501 ctcgtggcaa acgacggttt tgcgcccaac gaccattgcc gtccatgatg atggcaatgt
    22561 gctgaggaat tggtccatcg gtattaaatt gaaattcgct tttttcttga atatatttgt
    22621 ttttttgcgg aaaaaaacgt aacattcttt ttcctcctat acttaaaaga ttcccactat
    22681 cttaagtatt atagcaatat ctaactgttt ttcctatgaa atcattgttt ttttatttaa
    22741 aaactgaata attttttctt tttttaattt ttaaataaaa gaaatataaa taaaaacaga
    22801 ggaagcggaa tgatccgccc ctctgttttt atgattttat taaacttcca ataattcttt
    22861 ttctttatca gcagcgatct tatctacttc tttgatgctg ttatctgtca atgtttgtac
    22921 gtctttttct aatccgcgca aatcatcttc ggtaatatca ccattttttt gttgtttttt
    22981 atattcatcg atcgcatcac gacggatgtt acgaatcgcg atttttgcat tttctgcttc
    23041 ttttttaaca tctttagcta attcttttct gcgttcttct gtcagttgcg ggataactaa
    23101 gcgaatgaca tttccatcat tggttggact gattccgata tcacttgcca taatagcttt
    23161 ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc attaaaacac gtgcttctgg
    23221 aatttggatt gatgccattt ggttcaatgg tgttggcgca ccgtagtagt tcacagtaat
    23281 acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt tgcccaagtt cacgttgaag
    23341 attttgtgct gcttttttca ttttttcttt tgcttctgtc ataattgcat ctgccatttt
    23401 tatttccccc ttacagttgt tccgatattt tcgcctaaga ttgcacggcg aatatttccg
    23461 tgttcattta aattgaagac aagcagtgga atatcattat ccatgcttag tgaacttgca
    23521 gttgaatcca tgacttgtaa tcctttggca ataacttcca gatgagtcag ttcttcaaat
    23581 tttactgcat ttgcatctac tttcggatca gcagaataaa caccgtctac attgttttta
    23641 gccatcagga taacatctgc gccgatttct gccgcgcgta atgctgctgt tgtgtctgtc
    23701 gagaagtacg ggttgccggt accgcctgca aagataacga tccgtccttt ttctaaatga
    23761 cgttctgctt tacggcgaat atatggctct gcgatttgac gcatttcaat cgaagtttgc
    23821 acgcgtgtcg gtacacccac gttttccaac gtatcctgta aagctaaggc attcataact
    23881 gtagctaaca tacccatgta atcagcttga gcacgttcca tgcccatttg tgctccgatt
    23941 tgtccgcgcc agatatttcc gccgcccaca acgattgcca tttcaacgcc cagctcgtga
    24001 acttctttga tttcttggat gatttctttg attactggtg gtttgattcc aaatcctgcg
    24061 tccccagcta aagcttctcc actcaatttt aagactacac gctgatattt cggtgtgacc
    24121 atgttcattc ctccaatatt ttctattgcc attttaccat acttagatga tttcgacact
    24181 ctaagactgg tttttcttgt tttgcttaaa aaaagggagc gcacaaaccg tgcgttcccg
    24241 tctgttattc aacagaatta ttttttcact tggctcataa cttcttctac aaagttatct
    24301 tcgcgttttt cgataccttc gccaacttcg aaacgtacga atgttttaac agttgcgcct
    24361 tttgatgcaa cgtatttttc aacagtcata tcaggatctt taacgaatgg ttggtccacc
    24421 aaagcgattt ctgctttgaa tttcttcaag cgtccttcta ccattttttc aacgatattc
    24481 gctggtttgc cttcgttcaa tgcttgttct gtaagaacag ttttttcatg ttctaattca
    24541 gcttctggaa tttgtgattc gtttacataa cgaggattga tagctgctac gtgcatagct
    24601 acgtctcttg caactgtttc gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt
    24661 ccgcccatat gcaagtatcc accaaatgca gcattgtcgt ctttttctac aacttcaaag
    24721 cgacggaagc tgattttttc tccgataact tgagttgctt cgatcaagtc agattcaatt
    24781 gtacctttat ctgttttgat tttcattgca gcttccatgt ctgctggttt gttttcagca
    24841 acaagttctg caatttcttt tactaaatct tggaacattt cgtttttaga aacaaagtct
    24901 gtttctgagt ttacttcaac gattgctgca gtgttacctt tcacagcaac tgaagccaat
    24961 ccttcagctg caatgcggtc atttttcttc gcagcctttg ccattccttt ttcacgtaaa
    25021 agatcaactg ctttttccat gtcgccttct acttcaacta atgctttttt cgcatccatc
    25081 attcctacgc cagtcatttc gcgtagttct tttaccattt tagctgtaac gtctgccatg
    25141 tttaactcct cctgtgtgtt tttcttttta aaaaaagctg cttcaaagga gagtgaggct
    25201 tgtcgcccag cctttgaaac agctccatca ttcaaaatta ttctgctgaa gaattgtcgc
    25261 cttcaacaac atctacgatt tcttcgattg atgttgctgt gttttcttct gtgaaatctt
    25321 cttcaactac ttgatcttca ccttggtttc cttcgatgaa agcatcagcc atttttgaag
    25381 taatcaattt aacggcacga attgcatcgt cgtttgaagg gattactacg tcgatttcat
    25441 ctggatcgca gtttgtatca accatagcta cgattgggat atttaatttg tgtgcttctt
    25501 gaacagcaat acgttcttta cgtgggtcaa cgatgtacat tacatctggg attcttggca
    25561 tatcagcgat accgcctaag aatttttcaa gacgttcacg ttctttgttc aaaccagcaa
    25621 cttctttttt aggtagaact tcaaaagttc catcttcttc cattgcattg atttgtttca
    25681 aacggctgat acgtttttgg atagtatccc agtttgtcaa tgttccgcct aaccaacgat
    25741 ggttaacgaa gtattgtcct gcacgagttg cttcttcttt gattgcttct tgtgcttgtt
    25801 tttttgtacc tacgaataat gctacgccgc cttcttcagc aacgctcttc atgtaatcat
    25861 aagctgcatc tactaattta actgtttttt gcaagtcaat gatgtagatt ccgtttcttt
    25921 ctgtgaagat atatttcttc atttttggat tccagcgacg agtttggtga ccaaagtgta
    25981 cgccggcttc tagtaattgt ttcatagaaa ttactgccat gtttgtttcc tccgatttgg
    26041 ttttattttt cctcttctta tcttctacct tgccggcaaa ctttttcaag cacctaccga
    26101 caatccaata agaatgtgga tttgctgatg cataaagcac cctcagctat tatacagaca
    26161 aaagatccgt atcgcaagag caaatatact tttctgctca acattcaata gtttacctga
    26221 aagataaaaa gaattcaaga aaaacagtag atttctcttt gtttctttat tatccttttt
    26281 ccataaaata attttaggta taaaagacga atattattgg gcaatccttc ttttttttgt
    26341 tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt
    26401 tgctgattaa acaagtcatt gaagaattcc cgatccgtac gcaagaagaa ctattaaaca
    26461 aacttgggca ttatggcgtt accgccacgc aggcaacgat ttctcgagat atccgtgatc
    26521 taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa
    26581 aaggaactcc tgagactagg actgaggaag aggatcggct ggttcaaatg attgaagaca
    26641 ttgtattgaa agtcgaacga gtccactttt tgacgatcgt caataccttg ccagacaatg
    26701 ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc ctctattgtc agtacaattg
    26761 ctggattcga tacgatcatg gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag
    26821 aatttcttca ccatccaact gaagtatcac ttttttaatc agagcacgta ttcctaatat
    26881 ggaatgcgtg tttttttatt ttcaatattt tccttattga ataaaccttt acactcttta
    26941 caaaaataca tcatttttta ttcaaatttt tcaacacctt gattgaatca caataatcat
    27001 catttatatt ggatgttaac aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa
    27061 tgaaaggaaa tcatatgtat aaattattaa cttctggacc attgacgact tctgaaacag
    27121 tgaaaaatgc tatgctgatc gatcattgta cttgggatca agaatacaaa gaaattacac
    27181 aatggataag aaaaaaatta ttggatcttg ctcaagtatc ggaaggaata tacacaacgg
    27241 tcttaatgca aggtagcagc tccttcgggg tagaatcggt attaagttct gtgatcaggc
    27301 cagaagatac tgtactgatt tgttcaaacg gcgcatatag acaacgaatg atcaacatgt
    27361 gcgaatgtct gaaactgaac tataccgttt atgaggtatc ggaacatcaa atcctccaag
    27421 ctcatgtgat tacacagctg attgaagaga atccgtctat ctccgcacta gcaatggtcc
    27481 atagcgagac tacttccggt attttgaata atttggaaga gattgctgct gtcacacaag
    27541 aaagagatct tttgtttata gtggacgcga tgtcaagctt tggtgggata ccgatttgtg
    27601 tagccgaact agggattgat tttttgattt ccagctcaaa caaatgtatt caaggtgttc
    27661 caggcttttc ttttgtactt gctaataaag cggaactgga aaaacgaaaa aatgttagcc
    27721 gaagtttgtc acttgatcta tatgaccagt accgcgtgat ggaaaacgag aatggcaaat
    27781 ggcgtttcac ttcccctacc catacggttt tggcttttca acaagcgctg aaggaactag
    27841 aggcagaagg aggaatcgaa actcgatatt cacgttatct cgccaataat catgtgctac
    27901 gacaaaaaat gacagagatg gggttccagc ctttcattca agaatatcag gggccattca
    27961 tcacctcttt tctttaccct aaggactggc gatttgattt taatcatttt tatcagtatt
    28021 taaagcaaaa tggatttgtg atctatcccg ggaaaatcag cgatcatgaa tgttttcgta
    28081 tcggaaatat tggcgagatc tatccggaag atattttgga actgacagca attatcaaag
    28141 actataagga gaacgttaat gattaaaact gtaatttttg attgggcagg aacaactgtg
    28201 gactttggct gtatggctcc tgtccacgct tttagaaatg cttttctaga aaaaggaatc
    28261 caactgactg acaaagaaat ccgctaaccg atgggaaaat taaaatggga tcatatccaa
    28321 cagcttcttt ccctcccttc tgtcaaaaaa caatggatag ctatttatgg acaactacct
    28381 caggaaaaag aggtggagga attgtatcaa caatttgaac attatctgtt taaagatttg
    28441 gtcaagcatt ctaccttgaa acccgacaca ttggaaacga tcgaacggtt gagaaacgct
    28501 gggatcaata ttggttccac gaatggattt aatgctccta tgatgacaat cgtagcagaa
    28561 accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg
    28621 tatggtcgac cgtttcctta tatgatccat aaaaacatgc agtacttcaa aaacaagtcg
    28681 gttgatgaag taatcaaagt cggagatacg gtctcagata tccaggaagg gaaaaatgcc
    28741 ggagtgttga cagtaggtgt aattgaaggt agttcgctaa tggggatttc ttatgatgag
    28801 taccaaaaat tagagatggg gaaaaagatc gtttaaaaca gtctgttgct cttagattcc
    28861 aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt
    28921 acgctgacat gaatccagaa tgacttttat acaaaataag aggttgtgaa aaagctgtcc
    28981 tgtatcaagt aataaaaaca gccaagcaaa aatagctcct catatttttc gtttggttag
    29041 gcttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa aagggagctg
    29101 tgacacgact tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa gagacaacct
    29161 ctccactaaa cggtgttcat tcagctgact ctcggtattt gctcaaaaaa cgataaaatt
    29221 tgaaaagcta ttttctcttt tgtttctcta atactcggat cagaacgctg atttcacatt
    29281 ctctttttct tacatcttat ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa
    29341 taatacagtc actgcataaa ctagagctaa gcgtgcttct ttttgttcat catcagctaa
    29401 aatcttcgta tgagcatagt atttgttgaa tgcttgtgct aatttgattg catgtttcgc
    29461 aatcactgac ggttcgtatt tttccccagc agacaagacc gtttcagggt atttttgaac
    29521 taatttaacc acttcccagc tggtatcgtc attcaatgca tagtctgctg tttctgaagg
    29581 gacaaatcct gctttttcca acaggctgac tgcacgagca tgtgtatatt gcacataagg
    29641 tccagtttct ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg tattcagtcg
    29701 atcgtttttc aaatcgtgga aaatgactgc cccaacccca acttgtttag ctacagcatc
    29761 tttgttttcc aaatcagggt ttttctcgct gatttgttct ttagcagatt ggattgcttc
    29821 atttaatact tcttctaata agacgatctt tcctttacga gttgataatt ttttaccacc
    29881 ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa agcccatttc
    29941 cttcaagaca gctttcaact gtttaaaatg atagctttgt tcattcccta ctacatatag
    30001 agattgtttg aagtcgtaag tacgtttacg gtacaatgca gcagctaagt cacgtgtaat
    30061 gtatagtgtc gcaccgtctg acttcttaat caacgctgga ttcaggtcat aagcagatag
    30121 gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac
    30181 gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc
    30241 cagcagatca tagattttat tgaattcttt catcgattca tcacggaacc attgccataa
    30301 ttgaatagcc tctttatcgc cttcttccag acgtttgaac caagcacgag cttcttcatt
    30361 taattctggt tctgtttcag ctacttcatg gaattggacg tataaacgaa gcaattcatt
    30421 gattggttct gctttaacag cttcttctgt accccatttt ttgtaagcga cgatcagttt
    30481 tccaaactgt gttccccagt cacctaaatg gttgatgcgg attggctgat aaccgatttt
    30541 ttccataatg aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc ccattgagat
    30601 tggtttggca atattaggag aagacatgtc gattggtact gttccttgat tcccgatgtt
    30661 gctatcccca taatgttctt tttctttcac tactgtttgc aaaacttttt tgctgataag
    30721 ttctttattc atgaaaaagt tcaagtaagg tccgacaact tcgatttttt caaagttggc
    30781 agaatcgatt ttttctgcta aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt
    30841 tgctaatgaa aaggctggaa atgcaacatc tccgtgttca gctgattttg gattttctag
    30901 taattgctcg acttgttcca gtgttaaatc atctttgaca gcgtcataga cagcttttgc
    30961 gacaagatct ttattattca tctttattcc tcctaaagtg ttcccttgat tgttaaacaa
    31021 aaaaaatctc tagcgacggc atacttagta tgccatcact agagacgaga tttcccgcgg
    31081 taccactcca attgtcctaa aaaaggacca gctccatctg atatcggtag acgaccgtct
    31141 gtttaaaaca gaattctcca agtgcgcttc gttgtatctt cttcattcgg cttccaccac
    31201 ttccgaattc gctagccaag aatgatacaa gtactctctt gttcaacaaa tcttagtctt
    31261 attaaacttt cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa
    31321 acaatccaga tggcctaaag gagaagaaat accacctgga tagctataaa tcatttcgta
    31381 aatacgaaag acttaacaaa ttaatcagct acgataatcg ttccttcttc agaagctaaa
    31441 agattctcga tattttctaa agaagtgatg attgccttag cgtttggttt tgcttcaaca
    31501 aatgcaatag ctgcttctac tttcggaagc atgcttcctg gagcgaattg cttttcatca
    31561 atgtattgtt tcatttctga aacagtaaca gtttctaatt tcttttgatc tggtttttgg
    31621 taattgacat acacattgtc tactcctgtc aatacgatca gcaaatccgc atcgatgatt
    31681 tcggctaatt tttctgaagc aaaatcttta tcgattacgg cttctcgtcc ttctaatcca
    31741 gtagctgttt ctacaacagg aatcccaccg ccaccaacag agactgtgat aactccttga
    31801 tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga tgggtttagg ggaagcgact
    31861 acttttctcc atccacgtcc agcatcttct ttgaatgtca cagtggagtc agcattcatt
    31921 tgttctttgg cttcttcttc cgtgtagaat ggaccgactg gtttacttgg atttttgaat
    31981 gaaggatcat tttcatccac aataacttga gtgactaaag aaaccacatc tttatcgatc
    32041 cctttttcct tcagtacttc gcccatggcg ttttgtaacc agtaaccgat agatccttct
    32101 gtcattgcaa cacatgtatc taaaggcatt gccggtgttt tctcagaatc agcagcttgc
    32161 tgctgaatca ataggttacc gacttgcgga ccatttccgt gggagatgat caattcatct
    32221 ccttgttcga taaatttcac taaatacttt gctgtttcca ttaacgcttc ttgttgggct
    32281 tttgcacttg catccgtaga caagatggca ttgcccccta aagcgacaac tacttttcgt
    32341 ttggccattt cattcgtcct ttcttcagtt ctaccgaatt ttctagataa caaaacgagc
    32401 cagcgttttg tgattttccc tcaatgctga ctcgtttttt cgtgagatga atgtggttat
    32461 gcttaaacgc gagggataaa caagttacct aatgttgctg ccatgatcgc tttgattgag
    32521 tgcatacgat tttctgcttg gtcaaattga cgagcatatt tgctgcggaa gacttcatct
    32581 gttacttcca tttcagtgat gccaaagcgt tctttcattt gttcaccgta aacagtgttt
    32641 gtatcatgga aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt
    32701 tctaccattt ccatattgat ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta
    32761 tcttcttcac ccattgatac ccatacatca gaataaagga cgtctgctcc gtctacacct
    32821 ttatcaacat cgtcggtaat catcaattgt gcaccggatt tttcagcaaa gccttctgcc
    32881 attttgacga tttcttcttc aggttgtaat tcttttggtg caacgatacg catattcgct
    32941 cctaaaattg cacctgtgac taataaagag ttagccatat tgtttcgacc atctccgcag
    33001 taagcaacag tgataccttc tactgttcca aagttttctt ggatagttaa aaagtcagcg
    33061 atcatttgtg ttggatgcca ttcatcagtc aagccattcc aaactggaac accagagaat
    33121 tctgctagtt cctctaccat tttttgactg aatccacgga attcgattcc gtcaaacata
    33181 cgtcctagta ctttagctgt atcttctgtg gattcttttt tacctaattg gatatcattt
    33241 gcacctagat attcaggatg tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga
    33301 gtacgtgtag atgttttttc aaataaaagc gcaatatttt ttccttctag atagtgatgt
    33361 gggataccac gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa atattgtaat
    33421 tcttcttttg taaagtcttt ttctgctaat aagcttcttc cttggaatac tgattctttc
    33481 atattaaaca ttctccttta gattgttttt atttaagatt aaagaggaaa tcccgacggc
    33541 agctgacagt aagactgccg tcaggaaaca aattacttca agtcttcacg gacaagtggt
    33601 tggctcatgc agcgtggacc accacgacct cgtgacaatt cacttgaatt gatttctaac
    33661 actttgatgc catagctacg aagcaattcg ttcgatacat aatttcggtt ataagtgact
    33721 acaacacctg gagcgattgc caatgtgttt gatccatcat tccattgttc tcgaggtgca
    33781 acgatctcat ctccattacc agttgggatc agtacaagat catctaatcc aagagcagct
    33841 ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga tatcatcacc atctggttcg
    33901 attgtaaaga catccatctt accgtcttta ctttgaattg ctggatggat agtgaattta
    33961 tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt gttcggaatt
    34021 ttgattgcca acactttttc aaatcctgaa ttttttgcga ataggtggcg tgctaatttt
    34081 tctaaagctt tcgcattagt tcgttgagaa atcccaacag ctactacttt atcgcttaag
    34141 atcaattcgt ctccgccttc gatatgatct ggattttcac gatccaacca tacttcgatt
    34201 cctttatttg caaatcttgg atgatgtttc aaaatatatt ctgtaaacat cgattcacga
    34261 cgacgtgctt caaaagtcat cttattgact gtcatcccat ttcccattga tgcggaaggg
    34321 tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa atggatagtc atcgtctgct
    34381 gacagatcat atagactgct agaacgtact tcgatatctt ttgtacgaac cccagccatg
    34441 attttatcta ccatttcttg cgtttccatt gatagaagga actcatgaag accctctctt
    34501 acagcattcg atgcaatatg agattcatcc aacattttat tcaagaattg ttctttgaca
    34561 ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt aaagagtctc gacgccttca
    34621 ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag caatcggaag atatggaata
    34681 tcatcgaaca acagacgatc cataatatct ggtgtcaaat tttccacttc ttgtccaggc
    34741 cgtttcagta aaactgtctt taatttccct atttcagaga aaacgtgaat aggtttatcc
    34801 atgttgtgtt gcctccttct ttttgattac atgtatagga tatcctcctt ttgtaatcac
    34861 tttcataatt ttgtctcatt caaaatgaga ca
//
LOCUS       DASJRQ010000018        34664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJRQ010000018 DASJRQ010000000
VERSION     DASJRQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.018"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="U4A80_000828"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="U4A80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1974462.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="U4A80_000829"
     CDS             complement(2020..2922)
                     /locus_tag="U4A80_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1974463.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="U4A80_000830"
     CDS             complement(2919..3611)
                     /locus_tag="U4A80_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1974464.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="U4A80_000831"
     CDS             complement(3654..4295)
                     /locus_tag="U4A80_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1974465.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="U4A80_000832"
     CDS             complement(4333..4647)
                     /locus_tag="U4A80_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974466.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="U4A80_000833"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="U4A80_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="U4A80_000834"
     CDS             complement(5984..6916)
                     /locus_tag="U4A80_000834"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1974467.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="U4A80_000835"
     CDS             complement(6913..7689)
                     /locus_tag="U4A80_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1974468.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="U4A80_000836"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="U4A80_000836"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1974469.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="U4A80_000837"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="U4A80_000837"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1974470.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="U4A80_000838"
     CDS             complement(12014..13294)
                     /locus_tag="U4A80_000838"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1974471.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="U4A80_000839"
     CDS             complement(13313..14239)
                     /locus_tag="U4A80_000839"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1974472.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="U4A80_000840"
     CDS             complement(14252..15535)
                     /locus_tag="U4A80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1974473.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="U4A80_000841"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="U4A80_000841"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1974474.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="U4A80_000842"
     CDS             complement(16323..17234)
                     /locus_tag="U4A80_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1974475.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="U4A80_000843"
     CDS             complement(17231..17695)
                     /locus_tag="U4A80_000843"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1974476.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="U4A80_000844"
     CDS             complement(17700..18398)
                     /locus_tag="U4A80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1974477.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="U4A80_000845"
     CDS             complement(18385..18771)
                     /locus_tag="U4A80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974478.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="U4A80_000846"
     CDS             complement(18918..20588)
                     /locus_tag="U4A80_000846"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1974479.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="U4A80_000847"
     CDS             20771..21004
                     /locus_tag="U4A80_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1974480.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="U4A80_000848"
     CDS             complement(21103..21534)
                     /locus_tag="U4A80_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1974481.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="U4A80_000849"
     CDS             21613..22026
                     /locus_tag="U4A80_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1974482.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="U4A80_000850"
     CDS             complement(22064..23212)
                     /locus_tag="U4A80_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1974483.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="U4A80_000851"
     CDS             complement(23334..23930)
                     /locus_tag="U4A80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1974484.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="U4A80_000852"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="U4A80_000852"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1974485.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="U4A80_000853"
     CDS             27160..27591
                     /locus_tag="U4A80_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974486.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="U4A80_000854"
     CDS             complement(27636..28985)
                     /locus_tag="U4A80_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1974487.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="U4A80_000855"
     CDS             complement(29055..30218)
                     /locus_tag="U4A80_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1974488.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="U4A80_000856"
     CDS             30347..30670
                     /locus_tag="U4A80_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974489.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="U4A80_000857"
     CDS             complement(30853..32202)
                     /locus_tag="U4A80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1974490.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="U4A80_000858"
     CDS             complement(32271..33224)
                     /locus_tag="U4A80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1974491.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="U4A80_000859"
     CDS             complement(33326..34516)
                     /locus_tag="U4A80_000859"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1974492.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9848 a         7593 c         6088 g        11135 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat cttt
//
LOCUS       DASJRQ010000019        34448 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJRQ010000019 DASJRQ010000000
VERSION     DASJRQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.019"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            352..1041
                     /locus_tag="U4A80_000860"
     CDS             352..1041
                     /locus_tag="U4A80_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974493.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="U4A80_000861"
     CDS             1028..2236
                     /locus_tag="U4A80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1974494.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="U4A80_000862"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="U4A80_000862"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1974495.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U4A80_000863"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="U4A80_000863"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1974496.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="U4A80_000864"
     CDS             complement(6339..7034)
                     /locus_tag="U4A80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1974497.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U4A80_000865"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="U4A80_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1974498.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="U4A80_000866"
     CDS             complement(8233..8424)
                     /locus_tag="U4A80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1974499.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="U4A80_000867"
     CDS             complement(8445..9335)
                     /locus_tag="U4A80_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1974500.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="U4A80_000868"
     CDS             complement(9322..10089)
                     /locus_tag="U4A80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1974501.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U4A80_000869"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="U4A80_000869"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1974502.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U4A80_000870"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="U4A80_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1974503.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U4A80_000871"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="U4A80_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1974504.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="U4A80_000872"
     CDS             14660..15043
                     /locus_tag="U4A80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974505.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="U4A80_000873"
     CDS             complement(15211..15969)
                     /locus_tag="U4A80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1974506.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="U4A80_000874"
     CDS             complement(15987..16805)
                     /locus_tag="U4A80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1974507.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U4A80_000875"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="U4A80_000875"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1974508.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U4A80_000876"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="U4A80_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1974509.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="U4A80_000877"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="U4A80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1974510.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="U4A80_000878"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="U4A80_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1974511.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="U4A80_000879"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="U4A80_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1974512.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="U4A80_000880"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="U4A80_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1974513.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="U4A80_000881"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="U4A80_000881"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1974514.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="U4A80_000882"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="U4A80_000882"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1974515.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="U4A80_000883"
     CDS             26847..27146
                     /locus_tag="U4A80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1974516.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="U4A80_000884"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="U4A80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1974517.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="U4A80_000885"
     CDS             27734..27970
                     /locus_tag="U4A80_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1974518.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="U4A80_000886"
     CDS             28113..30086
                     /locus_tag="U4A80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1974519.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="U4A80_000887"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="U4A80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1974520.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="U4A80_000888"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="U4A80_000888"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1974521.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="U4A80_000889"
     CDS             32965..34257
                     /locus_tag="U4A80_000889"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1974522.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10510 a         6702 c         6821 g        10415 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaa
//
LOCUS       DASJRQ010000020        32795 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJRQ010000020 DASJRQ010000000
VERSION     DASJRQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.020"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            258..1814
                     /gene="rny"
                     /locus_tag="U4A80_000890"
     CDS             258..1814
                     /gene="rny"
                     /locus_tag="U4A80_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1974523.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1893..2699)
                     /gene="proC"
                     /locus_tag="U4A80_000891"
     CDS             complement(1893..2699)
                     /gene="proC"
                     /locus_tag="U4A80_000891"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1974524.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2840..5413
                     /gene="mprF"
                     /locus_tag="U4A80_000892"
     CDS             2840..5413
                     /gene="mprF"
                     /locus_tag="U4A80_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1974525.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5555..6418
                     /locus_tag="U4A80_000893"
     CDS             5555..6418
                     /locus_tag="U4A80_000893"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1974526.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            6663..7601
                     /locus_tag="U4A80_000894"
     CDS             6663..7601
                     /locus_tag="U4A80_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1974527.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7624..8304
                     /locus_tag="U4A80_000895"
     CDS             7624..8304
                     /locus_tag="U4A80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1974528.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8277..9101
                     /locus_tag="U4A80_000896"
     CDS             8277..9101
                     /locus_tag="U4A80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1974529.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9268..10086
                     /gene="purR"
                     /locus_tag="U4A80_000897"
     CDS             9268..10086
                     /gene="purR"
                     /locus_tag="U4A80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1974530.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10589..11962
                     /gene="glmU"
                     /locus_tag="U4A80_000898"
     CDS             10589..11962
                     /gene="glmU"
                     /locus_tag="U4A80_000898"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1974531.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            12056..13027
                     /locus_tag="U4A80_000899"
     CDS             12056..13027
                     /locus_tag="U4A80_000899"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1974532.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      13033..13135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13201..13770
                     /gene="thiT"
                     /locus_tag="U4A80_000900"
     CDS             13201..13770
                     /gene="thiT"
                     /locus_tag="U4A80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1974533.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13851..14624)
                     /locus_tag="U4A80_000901"
     CDS             complement(13851..14624)
                     /locus_tag="U4A80_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974534.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14891..16591
                     /locus_tag="U4A80_000902"
     CDS             14891..16591
                     /locus_tag="U4A80_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1974535.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16561..16779
                     /locus_tag="U4A80_000903"
     CDS             16561..16779
                     /locus_tag="U4A80_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1974536.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16799..17689
                     /locus_tag="U4A80_000904"
     CDS             16799..17689
                     /locus_tag="U4A80_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1974537.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17707..17991
                     /locus_tag="U4A80_000905"
     CDS             17707..17991
                     /locus_tag="U4A80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974538.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18478..19092
                     /gene="gmk"
                     /locus_tag="U4A80_000906"
     CDS             18478..19092
                     /gene="gmk"
                     /locus_tag="U4A80_000906"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1974539.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            19097..19402
                     /locus_tag="U4A80_000907"
     CDS             19097..19402
                     /locus_tag="U4A80_000907"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1974540.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19655..22063
                     /gene="priA"
                     /locus_tag="U4A80_000908"
     CDS             19655..22063
                     /gene="priA"
                     /locus_tag="U4A80_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1974541.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            22078..22569
                     /gene="def"
                     /locus_tag="U4A80_000909"
     CDS             22078..22569
                     /gene="def"
                     /locus_tag="U4A80_000909"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1974542.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22562..23500
                     /locus_tag="U4A80_000910"
     CDS             22562..23500
                     /locus_tag="U4A80_000910"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1974543.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23500..24858
                     /gene="rsmB"
                     /locus_tag="U4A80_000911"
     CDS             23500..24858
                     /gene="rsmB"
                     /locus_tag="U4A80_000911"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1974544.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24871..25611
                     /locus_tag="U4A80_000912"
     CDS             24871..25611
                     /locus_tag="U4A80_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1974545.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25608..27677
                     /gene="pknB"
                     /locus_tag="U4A80_000913"
     CDS             25608..27677
                     /gene="pknB"
                     /locus_tag="U4A80_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1974546.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27842..28741
                     /gene="rsgA"
                     /locus_tag="U4A80_000914"
     CDS             27842..28741
                     /gene="rsgA"
                     /locus_tag="U4A80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1974547.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28754..29404
                     /locus_tag="U4A80_000915"
     CDS             28754..29404
                     /locus_tag="U4A80_000915"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1974548.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29405..30043
                     /locus_tag="U4A80_000916"
     CDS             29405..30043
                     /locus_tag="U4A80_000916"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1974549.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30198..31052
                     /gene="mreC"
                     /locus_tag="U4A80_000917"
     CDS             30198..31052
                     /gene="mreC"
                     /locus_tag="U4A80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1974550.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            31056..31565
                     /gene="mreD"
                     /locus_tag="U4A80_000918"
     CDS             31056..31565
                     /gene="mreD"
                     /locus_tag="U4A80_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1974551.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            31820..>32795
                     /locus_tag="U4A80_000919"
     CDS             31820..>32795
                     /locus_tag="U4A80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974552.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT        10817 a         5828 c         7093 g         9057 t
ORIGIN      
        1 gaaaagtttc tcaatttaaa ggtttaccta tatgaaacta gtgtcattta ttttaaaaac
       61 catcgtttaa tgaataagta gaaagccgtt tctttgttta tttaaagttg acactacggg
      121 gtacaaacat tagaataaag ttgtgcatgt tttttgtaca catatatgcg aagatgcata
      181 ttgacgagtt tagaataatt tatcatagaa gctgtataga tgataaaaat gaaaaagata
      241 gtatggaggt ggattttatg gtattcaaca ttctcctcgc tatcatcggt ttaattgtcg
      301 gtcttggttt agggtttatg gtcgcaaagt cacgtcacga gaaagaaata gctggtgcac
      361 aatcatcagc tgctggaatc atcgatagtg caaaaaaaga agcagaaact ctaaaaaaag
      421 aagcattgtt agaagctaag gaagaaaatc agaaataccg ttctgaagtt gaaagtgagc
      481 tgagagaaag taagttagaa ttaaaatctc aagagaatcg tttgattcaa cgcgaacaaa
      541 cgctgaatcg taaagatgac tcgttagaaa aacgcgagaa ctcacttgaa agtaaagaag
      601 aagcacttag ttcaaaacag caattaattg aagagcgaga aaaagacgtt gaaaaaatga
      661 ttgaagatca acaaaaagaa ctagaaaaaa ttgctggttt atcaaaagag gacgcacgag
      721 atgtcatcat gaaatcgact gaagaagagc tgaatcatga gttaactcta atggtaaaag
      781 aatctgaaca gcgtgccaaa gaagaagcag atcgcaaagc gaaaaactta ttatcattag
      841 cgattcaacg ctgcgcagca gatcaagttt cagaaacgac tgtttcagtc gttacccttc
      901 cgaatgatga aatgaaaggt cgtatcattg gacgagaagg tcgtaatatt cgaacgcttg
      961 agacgctgac tggtatcgat ttaatcatcg atgatacacc ggaagcagtt gttctttccg
     1021 gattcgatcc aatccgaaga gaaatcgcac ggatgacctt ggaaaaactg atacaagatg
     1081 gacgaatcca tccagcgcgt atcgaagaaa tggtcgagaa atctcgtaaa gaaatggatg
     1141 aacgtattcg cgaatacggg gaagaggcag cgtttgaagt cggtgctcat acacttcatc
     1201 cagatctaat caagatcatc ggacgtctgc gattcagaac tagttatgga caaaacgtct
     1261 tgaagcattc ggtcgaggtt gcaaaattag ctggtatctt agcagcagag cttggcgaag
     1321 atatccaatt agcaaaacgt gccgggttgc tccatgatat tgggaaagca cttgaccatg
     1381 agattgaagg atctcacgtt gagatcggtg cggaattagc tgccaagtac aaagaaaacc
     1441 cagtagtaat caacgcgatc gcttctcatc atggagatgt ggaagctact tctgttatct
     1501 ctgtccttgt agctgcagca gatgcgctct cagcagcaag acctggtgcg cgaagtgaat
     1561 cattagaaaa ctacatccgt cgtttaagaa gtctggaaaa tatttcgaac agcttcgccg
     1621 gtgtagaatc aagttacgca gttcaagctg gacgcgaagt tcgggtaatg gtcaagccag
     1681 aagaaatctc ggatttagac gctgttcgtt tagtacgtga tattcgtaaa cgaatcgaag
     1741 aagaactgga ttatcctgga cacatcaaag taatcgttat tcgggaaact cgtgcaactg
     1801 attatgctaa ataataaaaa atacaaaaga agagccgcgg caaaagccac agctcttctt
     1861 tttttgtaag gcacgaaaaa acattgctat cattattttt tattcaacat ctcaagatct
     1921 ttctcgatcg tcgtattgat tcctttgatg attgctggga tgaatcctgc ttcttctaag
     1981 gcaactacac ctgcaacagt tgtaccgcca ggagaagaaa cttggtctac aagttcccat
     2041 ggatttttat cacttgaaag gaatttttga caagaaccca taattgcttt tgcagcaatc
     2101 tcagtggctg tctgtttgga caaaccattc agtacaccag tccgtcccat cgcatcgatc
     2161 agcatgtaaa cgaatgcggg tgagctgcca gcaaggccaa tgaatgtact gaaatctgat
     2221 tctgctaaag gatagacgct gccgattgct tcaaaaatca tcgtagcttg ctgaagctgt
     2281 tcctccgtgg cagcttcatt tttgcaaatc gcagaaacgc ctgctcctac gctgacattg
     2341 acattcggca tcacacgtac gatggaggcc tgaggatctt caagcgctgt ttctaaatct
     2401 attaatgatt ttcctgaagc aatagaaatg aataagactt tctggctata ttctcttagt
     2461 tcttccaata ctttttcaat gatttttggt ttcacggcta aaagaacgat atctgattgt
     2521 gatgcaactt cctgattact tttgcatgca tggacgccag tttcagcagc aaatttttgt
     2581 gcattttcaa aatgtccact ggaaacgaac acctcttctt tcgagactgt tccttttttt
     2641 aataaaccat caataatagc ttgtgccata ttccctgtgc cgataaatcc tactttcatg
     2701 ttatcctctc ctttagcatt aggatatctt tctttttcta tcatcgcaag caaagaacag
     2761 gtctaaagtg gaaaaattaa aataaataag ataacattgg ggtatgacac aaaagtatac
     2821 aagttaggaa tgggaaaatg tgaaaaataa acgatacttg atgctgcaat ggataaagga
     2881 acatagtctg attttaaaac tgattttttt cggctccgtg ttagtctttg tggcaaatca
     2941 agtcgcaaat attgctaatg gcatgagttg gcaagatatt tggcaaagga tgggacaaca
     3001 aagccgcatg acgatcctgc tcatgattct ggctggactg ctgggtgtca ctccgatgtt
     3061 gttgtatgat tgggtgacga tcagagtctt agaaaaacaa ggaaaaccaa aaatggaaag
     3121 aaaagagttt cttcttgctg cgtgggtaac aaacacgatc aacaatctcg ccggttttgg
     3181 tggtgtggta ggagcaagtc tgcgggctag tttttatgga aagcagacaa atagaaaaat
     3241 ggttttagcc actgtatcaa aagtcgcact atttatgctg actggtttgt ctgtatggtc
     3301 gtttcttaca tttattgatg tcttttttat tcagtcagaa agtatttttc gaagctattg
     3361 gatctggctt ttcggcggta gttttttagc accagtcatt ttcttttttg cttatctaaa
     3421 gagaaagaca ttgttcttcg agttttatcc aaaaggcttg cttggtctgc tactggcatc
     3481 ctttggacaa tggacgggag cccttgcggt atttttgatc atcggcaagt tgatgagaat
     3541 agatatttcg atgctttcga tctatcctat gttcatgatt gctacattga taggaatgct
     3601 gacgatggtc ccaggtggaa tggggacttt tgacgttttg atgatcttag gaatgagtca
     3661 attgtctgtt cggcaagatg tagcagtagt ttggctatta tattatcggt tgttttatta
     3721 tgttctacct tttatgacgg gacttctatt gttcatcaca catacaggag tcaaaatcaa
     3781 tcgattttta gataacattc cgcgactgtt tttccaaaag gttgcgcata tcgtgctcgt
     3841 aagtgctgtc tattttgcgg ggatcatgat ggtacttcta tcaacaatca ctaatctgtc
     3901 tgcaatcagt cggctatttc aagtattatt gccgttctca ttcaattttt tagatcagac
     3961 agtgaatcta ttggttggct ttttattact cggccttgct agagcacttt cagtaaaagt
     4021 caaaaaggca tttctgccta cgattgcttt actatctttt ggtattttaa atacaatcac
     4081 acgtacgtta tcttggcagt tgatattggt atatgtcttg attctgttag ctgtgtggtt
     4141 atccagaaaa gaattttatc gaacaaaatt cgtttattct tggggagcta taatctttga
     4201 tgctagcttg ttcggctttt tgtttatcgt ttacgcaatt gcaggctatc acagcggacg
     4261 ctttttagtc ggtcgaataa caggaaatca ttttattttg tttccttcag atgatgtgtg
     4321 gttctcaggg cttattggtt tgggcatctc attgctagct ttagtggcgc tttatcaata
     4381 tttagctgaa gatactacga agcttggcag tgagtacgaa aaagaccgtt ttgaccgttt
     4441 attggaaaca tacggaggca cagctacaag ccatcagctt cgcttgtcag gttacagtta
     4501 tttttattac caagaagacg accaagatca agtcgttttt ggttatcaga tcaaaggaaa
     4561 caaatgcttt gttttaggag atccagttgg caatcctaaa atgatgcggg cagcaacgaa
     4621 tcagtttatg aaacaagcag attgtttagg ctatcagctt gcgttttata agatatcaga
     4681 aagctacgta gtgatgcttc atgaattggg ttttcacttc acgaaagtag gtgaagcagg
     4741 gattgttgat ctagcaacag aagacttgtc tcggctgagt cagcaacttg aatatcaacg
     4801 tttgatgaaa gaaggatacc attttacttg gtacgatcaa ctgccggaag aattacatca
     4861 agctgtaaat gaagtttcac aaaaatggct tcaaggtgca cgagaaaaat acttctctgt
     4921 gggacgattt gataaatcat atgtattatc tagcggtgtc ggtattgtcc gtaaagatca
     4981 aaaagtcgtt ggcttcatca ccgctcagcc gatgacgaag aagcaggcgg gatatgattt
     5041 gttgcgggtc ttacctaaag aatctgagga attagctgac tatctgttag ccaatctgtt
     5101 tattgtatac gagaaaatgg gctactcgga agccaatcta ggcttagctc cattagctaa
     5161 tgttggagaa acagattttt ctttctttca agaaaaagtc atgcatatcg tatataatta
     5221 tggtaatttt ttctattcct tccagtctgc ttatgaaaag aaattaaaat atgtcgatca
     5281 ttgggaagga cgttattttg cttataggaa aggaagtaat ttcggatttg ctaccatgca
     5341 gctactcagt ctgataggaa aagaaaaagg aaatgttccg agtttggcag aagaagtgct
     5401 cacagaatta taagaatcac tagaacttgt ttggattatg aagagaaaat tctgtgaaag
     5461 ctgttgaaac gatgacaaaa tcaaccacta ggcagacata agctatactt ttatttttcc
     5521 ctatgataga atacttgcga cttagacttt tcgaatgggt gagaggatgg aattgattga
     5581 aaaagcgcca gccaagataa atttaggact ggatgtatta tatagacggc aagatgggta
     5641 tcatgagttg gaaatggtga tgtcgagcgt agacttggca gatcatctat tttttgaaga
     5701 aataacagaa gacaaaatca ttgtagaaac aaataaagca tttttaccgg tagatgaacg
     5761 gaacaatgtt tatcaagctg ctttattgct aaaaaataaa tacaatatca aaaccggtgt
     5821 caagatcttt gttaagaaga atattccagt agcagctgga ttaggcggag gaagtacaga
     5881 ctgtgcagct gctctaagag ggatcaacca attatggaat ctgggctgtt cgttagaaga
     5941 actggcagaa atcggtttgg aagtcggtac agatgttcct tactgtgtct acggaagtac
     6001 ggcatttgtc ggtggcaaag gggaagaaat ccaagtatta ccttctatgc cacagtgctg
     6061 ggttgtgttg gtcaaaccaa gaatgagtgt ctctacacgt acagtgttta gagaagtaga
     6121 tacgcaagcc atccaacatc aagacatcca cggacttgct gaagccatcc gagagcaaga
     6181 ttatcagcag atgatcaaaa aaatgggaaa cagtttagaa gatataacgg gaaagcggca
     6241 tccagtcatc catcaaatca aagagcgtat gatcaagtac ggagcagatg ctgcattgat
     6301 gagtggaagc ggaccaaccg tttttgcctt gtgtcaacat tattcaagag cgcagagagt
     6361 atataatggg ttaaaaggtt tttgtgagga agtttattta gtacgaaccc tgcaataaaa
     6421 tataagaagt aaaataggtg atgaaaaact gaggaatcct aaaatggtat tcttcagttt
     6481 tttattgtag tgaaatggct agatatgagc agcaactcat ccattttacg atttaatcaa
     6541 gaatatacat gcttaaatca gaaattgatg gatttctatt gactgtgttt ttttattttg
     6601 atacattagt tcacgttaat aaatcgtaaa attccgattt gttcattgaa aggagcatat
     6661 aaatgaaaaa atatcttttg acagctacgg taatgatagg tgctttactg tttgcagcat
     6721 gtggaaatac gaacaaggaa gcagacaaaa aagaggattt gacgatcgtt acgacttttt
     6781 atccgatgta tgattttaca aaagagatcg taggggatga aggaaacgtg aaacttttga
     6841 tccctgctgg aacggagccg catgatttcg aaccaagtgc aaaagaaaga gcagaaattt
     6901 ccgatgcaga tgttttcgtg tataatagtt cagacatgga attttttgtg gattcgttaa
     6961 aggattcagt ggatagcaaa cagaccttga tgatcgaagc agctaaagga atcgaccgtt
     7021 tagaatcgca agaagcagac gaacatgaag aatcagaaga agggcatggt cattcacatg
     7081 aatacgatcc acatgtttgg ctcgatccgg tattagctat aaaagaagtc agaacgattg
     7141 ctgaagaatt aggcgaaaaa tatccagata aaaaggaaac ttttacgaag aatgccgatg
     7201 cttatatcaa aaagcttgaa gcactggatc aaaaatactc cgatgagcta aaagatgcaa
     7261 caaaccgcac atttgtcact cagcatgctg cttttgctta tttagcgaat caatacgact
     7321 taaaacaagt agcgatttcg ggcgtttcac cagatcagga acccacacca agtcgcttag
     7381 ctgagctaaa agaatttgtg aagaaaaaca acatcaaagt gatttatttt gaagaaaatg
     7441 cctcttcgaa ggtagctgag acattgtcga atgaaacagg agtgaagcta gaagtgttga
     7501 atcctttaga aagcttgact aatgaacaaa tcaaagcagg cgaaaattat atttctgtca
     7561 tggaaaaaaa tctgaaagct ttaaaagaaa gtatcaatta agttttagaa tggaggttgt
     7621 aaggtgcgtt atatcgaaat atctgatttg accttttact atgatgatga acctgtacta
     7681 gaagatgtct cttatcatgt agatgcgggt gaatttgtca ttttaactgg agaaaatgga
     7741 gcagccaagt caaccttggt gaaagcatcg ttaggattac tgaaaccttc aaaaggaatg
     7801 ataaagattg ctgagaaaaa tcaagaagga aaaaaattaa gtatcggtta tattcctcaa
     7861 caagttgcat ctttcaatgc aggttttcct agtacggtga tcgaattagt ccgttcagga
     7921 agatatccaa gaggccgctg gttcaagcgt ctctctgaaa aagatcatca acatgtacag
     7981 cgtgctttag aagctgtagg gatgtgggat atgcgaaatc ggcgaatagg tgaattgtct
     8041 ggcggacaaa agcagcgaat cagtttagcg cgtatttttg ctactgaccc tgacttattt
     8101 gttcttgatg aaccaacaac aggtatggat gaagaatcca gaaatgaatt ttatcgtctt
     8161 ctccgacata gtgcgcatgt ccatgggaaa tcagttctaa tgatcaccca tgatcatgaa
     8221 gatatcaaac agtatgcgga cagacagatc cgactggtga gaaaggagga ttctcaatgg
     8281 cgttgctttc atatgaattc atgagaagag cgtttcttgc cgctatcttc attgccggaa
     8341 tcgccccgat gctaggtgtg tttttagtga tcagaagaca atcgctgatg gctgatacgc
     8401 tttctcatgt ctctttagca ggggtagcgt taggattctt tttgaacctt aatcctacac
     8461 ttacgaccat gctagtcgtt gtattagcag ctgtgatatt ggagtatctg cgaacgatct
     8521 accgaaccta ttcagaaatc tccattgcca ttttaatggc tggtggtttg gcgctagcgt
     8581 tagtattgat gaatttgagc ggagggaatt cagcaacaag tatccaatcc tatctgtttg
     8641 gttcgatcgt gaccattaca tgggagcaag tcatcttttt aggaatctta ttcgttgcca
     8701 tcggtatatt gttcttttta ttcaaacggc cgatgtatgt tctgactttt gatgaagata
     8761 ccgcccacgt ggatgggcta cccgttcatt tgatgtcgat gatgttcaat gtgattactg
     8821 gtgtggctat tgctgtgatg atcccgattg ccggtgcact tttgatatcc gcaatcatgg
     8881 ttcttcccgc agcaatcggt atgcgattag gcaaaagctt caatgtcgtg attgctatca
     8941 gtgtttgtgt aggatttcta gggatgctgt caggactaac tagctcgttt tatctagata
     9001 cacctccagg agcaacaatc acattagtct ttattgcttt gttcctgatt gtaaatatca
     9061 tcaaacgaat attggttgcc ttcagaagaa aaaataacta aattataaag aaaagaagcc
     9121 ttataaaaac gaaaaatttt ttataggctt ctttttattt taataaatac cgaactattt
     9181 tcttattttt tcataaaatg ctcgaatttt tgaagaaaaa cttatataat gtaaaacgat
     9241 aaaatttctt aaaaggagtt ggaataggtg aaagtacgtc gcagtgaacg tctagttgac
     9301 atgacacagt atttattgaa tcacccgcat gaattgattt ccttaacaag ctttgccgaa
     9361 cgttatgaat ctgctaaatc ttctatcagt gaagatttgg gaatcatcaa gaaaacattt
     9421 aaagaacgag ggtatggaac cttggaaaca atcccgggtg ctgcaggtgg tgtattgttc
     9481 atccctgaaa tttcatatga agaggcgaaa aaatatattg aatctctagc agaacgtcta
     9541 tcagaacaag accgtctgct tccaggagga tatgtttatc tctctgattt gttaggagaa
     9601 cctgaactgc tgcgtcaagt tggaagaatc attgcttcta aataccttgg tgaaaaaatc
     9661 gatgctgtaa tgactgtagc cacaaaaggc gtacctattg cccaagctgt gtcctattat
     9721 ctgaatgcgc cattcgttat tgtacgccgg gactcaaaaa taacagaagg atcaacagta
     9781 agtgtcaact atgtgtctgg atcttctgaa agaattgaaa agatggaact gtctaagaga
     9841 agtttgaaac gtggttcacg tgtccttgta gtggatgatt tcatgaaagg cggcggaaca
     9901 gtcaacggaa tgaagagctt gattgaagaa ttcgaatcag aacttgttgg catcactgta
     9961 tttgccgaat caaaattcaa cggtcatcgc gccatcgatg attatacgtc actgctttat
    10021 gtcaaagatg tagatactca aacgaaaaat attacggttg tgccaggaaa ttatttttca
    10081 gaataagagg ttgtgaaaaa gccgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat
    10141 ggctttccac attttttcat aaatttggct taataccgag aaactggctt ttgaacaccg
    10201 tttatctaga ggttgtgaaa tcagcgtcct gtcctgagta ttagctcaat aaacggaaaa
    10261 attgctcctc atattttgcc gtttattgag ctaataccga aggtcagctg attgaaaaac
    10321 gtttaatgaa gaggttgtaa gcctaccgtt taactctaag tcacaagaaa cgatttcaaa
    10381 aaacagcttt tcatgtttta tgagtagtgt gacttgtgac cgaggagttg gcaggagagc
    10441 accggttacc aaatagatag gcccgttatg gtctatctat ttttttatcc agaaaacgct
    10501 gggataaaag aaaatcactt gttttgttga aaaaatgggt atactgggat aaactgaaat
    10561 cagcaaacta tatttaggag tgacgcagtt ggacgcacgt tatgcaatta ttttggcggc
    10621 agggaaaggt acccgcatga agtcaaaatt atataaagtt ttacatcccg tttctggtaa
    10681 acctatggta gaacatatca ttaatcgggt cagtgaaacg aaaccagatg aagtgattac
    10741 gatcgttgga cacggagcag aacaagttaa agcacaacta ggagaacgca gtaaatatgc
    10801 gttacaagca gaacaattgg gaacaggaca tgctgttttg caagctgctt ctttcttgga
    10861 aggaaaaaaa ggaacaacgt tagttatcag tggcgataca ccattattga ctactgaaac
    10921 gctgaacaat ctttttgaat atcaccaagg aaaaaatgca agtgcaacga tcttgacggc
    10981 acaagcagaa aacccaacag gatatggacg aatcatccgt gaccatattg ggatcgtaga
    11041 aaaaatcgtg gaacaaaaag atgcaacacc agaagaagct cttgtacaag aaatcaatac
    11101 aggaacttac tgttttgaca acgaagcttt gttcgatgcg ttaagcaaag ttggaacaaa
    11161 caatgctcaa ggtgaatact atctaacaga tatcatcgaa atcttaaaag aagaaggcca
    11221 tacagttgct gcttatcaaa cggatgattt tgaagaatca atgggagtca atgatcggat
    11281 cgcgttagct aaagcaaatg agatcatgag aaagcgaatt aaccagatgc atatggtcaa
    11341 tggcgtaagc tttgtcgatt cagcaacgac atacatcgat gcaggcgtag aaatcggtcc
    11401 agacacattg atcgaagcag gtgtacaaat ccaaggaaat acagtcattg gttctgattg
    11461 tgtcatcggg tcacattcaa aaatcgtaga cagtaggatt gaggatcacg tcgtcattga
    11521 aaactcagtc attgaaagca gccatgtcaa gaaacatgca gatgtgggac cttatgcaca
    11581 tttacgacca aaagctgaaa ttggcgaaaa cgtccatatc ggaaactttg tggaagtgaa
    11641 aaatgctcaa atcggcaaag gaactaaagt cggtcacttg acttatgttg gtgatgctac
    11701 gctgggagaa gaaatcaatg tcggatgtgg tgttgtcttc gtcaactatg atggaaagaa
    11761 caaacatcat acgactgtcg gcgaccacag ctttattggt tctagcacga atatcatcgg
    11821 acctgtcgag gtagctaaaa acagttcgat tgctgccggg tcaacaatta cagacaatat
    11881 tccagaatat gcgctggcaa ttgcgagagc aagacaagtc aataaagaag gatatgcaaa
    11941 aaaactccct tatctaaact aagacttttg caaaatcact tgatttattt aaaaaatatg
    12001 actactattt attaggtaga attctagagt tgtgaaaaag aaagtggagg tccccatgtc
    12061 aaaacattac tttgacccaa gattaaaaat ttttgcgtta aattccaatc gcccattagc
    12121 agagaaaatt gcagcagcag tcggcgtcga gttaggaaaa tcatctgtta ctcaatttag
    12181 tgatggagaa attcaagtaa acattgaaga aagtattcgt ggatcgcatg tctatgttat
    12241 tcaatcaacg agcagcccag tcaatgataa cttgatggag ttgctgatca tgattgacgc
    12301 actgaaacga gcaagtgcaa aaacaatcaa cgtagtcatg ccttattatg gctatgcgcg
    12361 tcaagaccgt aaagcacgtg cacgggaacc aatcacagcg aaattagtag caaacatgat
    12421 tcaaaaagct ggtgcaacaa gaatgctgac tttggacctt catgcagtcc aaatccaagg
    12481 tttctttgat attccagtcg atcatttgat gggtgcgcca cttattgcca attatttctt
    12541 agaaaaagga atcaaaggcg atgatgttgt cgttgtttca ccagaccatg gtggtgtcac
    12601 acgggcacgt aaattagcgg aatttttgaa atctccgatt gcgatcatag acaaacgccg
    12661 tccaaaagca aatgttgcag aagtaatgaa tatcattgga caagtcaaag gcaaaacttg
    12721 tgtactgatc gatgatatga tcgatacagc aggtacgatc acattggctg ccaatgcttt
    12781 gaaagaagct ggtgcaacta gtgtgtatgc ttcatgtaca catccagtat tatccggccc
    12841 tgctttacaa agaatagaag attcagctat cgagcgtcta gttgttacag actcgatcta
    12901 tcttccagaa gatcgtaaaa tcgacaaaat cgatgaagta agtgttggcg gactaatggg
    12961 agatgcaatc aaacgtatcc atgaaaacaa accagttagt ccattgtttg aaacaaaaaa
    13021 taaataataa ataaactcac aaaggggagt ccaattgggc tgagattgaa tctatttcta
    13081 aacccttcgt acctgtatcg gttatgcgag cgtaggaatt gtgaataaac agctgtttga
    13141 ctattttcga aagctgcttc ctcctttgtg acttttttat tcaaaggagg aagttttttt
    13201 atgggaaaaa atagagtttg ggtagaagga acgattgttg cagcgttagc gatggtgttg
    13261 tcgttgattc cgatccagat cggcagcagt ttttctattt cattagggca gattccattg
    13321 actctttttg cgttaagaag agggacaaag gcagggatgc tggcaggttt catttggggc
    13381 atcctacatt ttccgttggg acaagtttat tatttaagtg tcgttcaagt gttgattgag
    13441 tacccgattg cctatacttt tgcaggcgca gcagggctca tggcttcaaa tgtccaaaaa
    13501 ggctatgtag acgataatac gaaacaaatt agaaaaagca tcgtgtttgg tgcattgatt
    13561 ggcgcacttt ccagatattt ttggcatttt attgcaggag tggttttctg gggagcgtat
    13621 gcactatggg ggatgaatcc ttggctcttt tctttcgtaa tgaatggtgc cagcgggttg
    13681 agtacagcta ttgtaacgat catcgtttta ttagggatcc aacaaacgac tccttcgatt
    13741 tttgtgccag ataaacgaca ggagctgtaa ataaaaaaga agcaagaaac ttgagacaga
    13801 atcatgtcct agtttcttgt ttccttttta ttttttgata agaaatagat ctacttcaat
    13861 ggcagataag aaagatcggc atcttctacc aattgccagt gtcgatcggt gacagagtag
    13921 attaaagtag cgatgatttc ctcttctcga taccgggtgc cttttaattt ccaaatataa
    13981 tcttcaatca catagattgg gatacgatat ttaccatcga tcaaaccaac ttgatcaaaa
    14041 gaaaaaatga tcccttcctg catcaaatta cgcatttctt tcaataattc cacactgatt
    14101 ttttcaacag gtgtttcttt tttgaaaaaa gattttttta cattgatctt atctgacaag
    14161 atcgaactta atttactagc aaaaatccta taaaattcat ctaaatagcg ataataaatc
    14221 cgattcagct gatcattaag atcgaagtat acaaaattat tctgtagttt ataaaaaaac
    14281 ggcgaatgaa gatgtgtttt catatgacca aaatataata attctgaaat ttctaaaggt
    14341 gtcaattctt tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca
    14401 cgcctgttac gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc
    14461 agccctgtat gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt
    14521 tgaggaaaat gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca
    14581 gcattacttg tggtatcaat atgtacataa actgactcag tcatcatgtt cctcctctta
    14641 tttcctctta atctctttta ttttacaaca tttgcattaa aaaagcttgt ttcttttatt
    14701 ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcattttc aattaatttt
    14761 tgtgattttt gtattttttt atttaaagga ggttttatga attgttttct tatggaatct
    14821 acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact tgaagaaaaa
    14881 ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc
    14941 tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa gtagagacgc
    15001 tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt
    15061 ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt tgattgaagg
    15121 tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc gtttttttat
    15181 taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt tgcgtatttt
    15241 tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct atcatacagc
    15301 acttgtttta ggtggaggag gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct
    15361 gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg cgttgaacgg
    15421 cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac
    15481 aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta
    15541 tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt cttctgctcc
    15601 attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata atcccaaaat
    15661 acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga ctgatatcca
    15721 acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat tttatcccgc
    15781 tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat
    15841 acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag atgtgcaagg
    15901 gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga aatgtcaaac
    15961 attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat taaatttaca
    16021 attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact ggtatacatt
    16081 tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact taacaaaaga
    16141 aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt
    16201 atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac ttttagcaaa
    16261 aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc aaatgatctg
    16321 ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc aattaagtac
    16381 tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac
    16441 tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc atgaattgct
    16501 taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg aggaacaacc
    16561 atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac
    16621 aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc aaaattcgat
    16681 cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa
    16741 gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac cctgtcgaat
    16801 gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc agttagatat
    16861 tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc ctaaaatcct
    16921 gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc gcggtcgaat
    16981 cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg tcatcaactg
    17041 ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac gatacggtag
    17101 tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gtgctggacg aaaacttcgt
    17161 aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag ctctagatac
    17221 attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc aagctttagc
    17281 tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt
    17341 tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg aggaatttct
    17401 tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt tggaacgcgg
    17461 agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag atgaacttat
    17521 cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa tgaatcggga
    17581 aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc
    17641 taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac gaaagaggta
    17701 gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa
    17761 cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg tgcagactat
    17821 aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat gaatcggaaa
    17881 tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc acgttctcga
    17941 aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta gcaaggatgc
    18001 tgaacaaaga aatacaataa ataaacagaa ttggatttct tcgcttagct tctttgctct
    18061 aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa
    18121 gtgatgttca aaaaccattt tctcggtatt aagccaaatt tatgcaaaaa tgtgagaaac
    18181 tattttcaca aaatttttct tcatactcga aactaatcgg cttcttcacg acctcttata
    18241 cacggcgttc aaaagcttgc atctgcgata ataaccccaa ccaaacgaaa aacatgagaa
    18301 gctgtttatg tttggctgtt cttattactt gatgcaggac agctttttta cgacctcttg
    18361 gtacagggta gcttttttac caatttctag ctataattaa agataacctt gaaagttgca
    18421 gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta gttgaaagga cgtcgtcatg
    18481 tcagagcgtg gattattaat cgtgttgtct ggtccatccg gtgtaggaaa aggcacagta
    18541 cgaaaagcaa tttttgagag tgaggataat gattttcagt attcgatctc aatgactacc
    18601 cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt tcttccgaag caaagaagag
    18661 tttgaatcaa tgatcgaagc tggagagatg ctggaatacg cagaatatgt cgggaattat
    18721 tatggaactc ctttgtcata tgtcaacaag acattggatg aaggaaaaga tgttttcttg
    18781 gagattgaag tccaaggagc aaaacaagtc aaagaaaaag tgcccgatgg tgtattcatc
    18841 tttttgactc cgccagattt agcggaatta aaatcacgga tcgtaggccg aggaacagat
    18901 gcagatgaag taatcgaaaa acgaatgaaa gttgcaaaag aagaaatcga aatgatggct
    18961 ctttacgact atgctgttgt aaatgatgaa gtgccgctag cagttgaacg aatcaaaaac
    19021 attattgcca gtgaacattt tcgagtagat cgcgtaatcg gtaaatatat taaaatgtta
    19081 aaggagatgt agtcttatga tgttaaaacc ttcaattgac tcactattag atcgtgtaaa
    19141 ttcaaaatat tctctagtga ttttagcaag taaacgcgcg catgaattgg atgcaagtgc
    19201 acagcctact ctagattctt ttgattcagt aaaaagcgtc ggtcaagctt tagaagaaat
    19261 cgatgcagga aatgtagtca atgatccaca tcctgaatta aaacgtgaac gtttgaagat
    19321 ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa cacgaattgg aatcacgtat
    19381 ccgtgaagag caaaaaatgt aataaagaaa aatagagcga acatctaaaa agaaaagcaa
    19441 acccagctgt ttccttttca aacattcgcc acttattgtc tggtagaaaa ggctgtgtct
    19501 gaactttttg acacagttct tttttttctt atctgctgtt aattttttgt taggatttgg
    19561 cagaagataa gacaaatcca tcgatctcta gtaaaatagg caaggaactt tttgtcgctt
    19621 gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca ttaacagctg aagtcattgt
    19681 ggacgtacct actatgcaga ccgatcagcc attcacttat ttggttccct ctgaaatgga
    19741 aacagctttg caagtcggga tgcgagtaga agtccctttt ggtaatggca atcgtcatgt
    19801 ccaaggattt gttatggcta tccaaaaatc agaagaatca gctaatccat ctttgaaggc
    19861 agttattcgt ttgctagacc ttgctccggt ggtcaatgaa gaattacttt cattagctga
    19921 ttatatgaaa aaaattactt acgcattcaa aatcacttgt ctacaaacga tgcttccgag
    19981 tgtgatgaaa gcagaatatg ataaattgat ctatcctttg gcagacacac cagaagttca
    20041 ggtattgttt gatcagcgag aagtgatttc ctggaaggaa gcagaagaag aaggcagcct
    20101 ttctcaattg attcgctggc gccaagagca attagtagat atcaaatatg aagtccatac
    20161 acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg ttaactgaaa agcaaatcga
    20221 agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa aaagaacttc ttttgtgttt
    20281 atctgaactg agccaagaag aaccaattgc ttatttcaaa aataaaggaa tcagtactgc
    20341 tgtcttgaat caaggaaaag aaaagggctg gctggagttt atagaatcag aacgttatcg
    20401 cgacccttat aaagaccgag tatttgacca aacgacggca ttggaactga atgcagaaca
    20461 aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag caaaaagatc aggtcttttt
    20521 gctggaggga atcactggca gcgggaaaac agaagtatat cttcaggcaa tcgctgatgt
    20581 tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa atcgcattga ctccgcaaat
    20641 ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg gtcttacata gcggactttc
    20701 ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg gaagaagcgc aagtagtagt
    20761 aggcgcacga tctgctattt ttgccccgct gaaaaatatc gggctgatca ttatcgatga
    20821 agaacatgaa tcaagttaca aacaagatga gacaccgcgt tatcatgcga gagatctagc
    20881 aatttggcgc agtaaatatc atcattgccc aatagttcta ggaagcgcga cgccctcttt
    20941 agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta ttgcagttaa accaccgagc
    21001 caaagcagct gcacagcttc cagctattga gatcgtagat atgcgtgaag agtttcagaa
    21061 tcatcggacc tctacttttt ctgctaattt gcaggaaaaa atacaaaatc gattagacaa
    21121 aaaagaacaa actgtcctac tgctcaatcg tcgaggatat tcgtcttttg tgatgtgtag
    21181 ggactgtgga tttgttcttc catgtcctaa ttgcgatatc tctcttacac tgcatatgga
    21241 tacacgttca atgcgctgcc attattgcgg acatgaagaa cctattccca atcgctgtcc
    21301 taattgtggc ggcaataaaa tccgctacta tggaacaggt actcaaaaag tagaagagga
    21361 actccgagaa ctttatccgc aagctaggat tttgcgtatg gatgtagata ccactcgaag
    21421 aaaaggggca cacgaacaga ttttacaaaa attcggtgca aaagaagcag atatattatt
    21481 agggacacaa atgattgcta aaggtttgga ctttccagaa gtgacattag ttggggtgtt
    21541 gaatgctgat acctccctga atttaccgga ttttcgttct agtgagcata cattccaatt
    21601 gcttacccaa gtttcaggac gagcagggcg agcggaaaaa gcaggcgaag tcgtgatcca
    21661 aacattcaat cctcaacatt acgccataga attggctaaa aaacaaaatt atgagcagtt
    21721 ttatcagcag gaaatgcatg tacggcatcg tggaggctat cctccttatt actttactgt
    21781 gaaaataacc gccagccatc ctgaagaaca agtagcggca aagaaaatct ttcagattgc
    21841 caatcagcta aaagaagtgt taagccctca aagcttgcta ttagggccaa cgcccagcat
    21901 gattttgaga gtgaaaaacc gatattatta tcaactaatc atcaaatata aacatgaacc
    21961 gaatcttcct catgttttgg atgaaatttt gaatggaagc cagaaagaac agcgacaagg
    22021 tctttttgtg gcaattgaca acgaaccatt aaactttatt taagaaaggg ccaaattatg
    22081 cgctatccta tattgatcca ccctaatgat aaattaaaaa gaactgctca gccaatcgat
    22141 gtaatcacgg acgagacaat cgctttatta gacaatttat atgagacaat gattgcgaat
    22201 gacggaatag ggatcgctgc tcctcaagta ggacaaaaca agagaattgc tgtgatcgaa
    22261 gtagatgaag gcgagaaatt tgaactgatt aacccagaaa tcatcgaagc aaaaggtgaa
    22321 agccttgacg tagaaggttg tctcagtatt ccgcatgtct atggaacggt caagcgagca
    22381 gatgaagtga ccgtgcgtta ttatgatcga gacggagaag agatagaagt gacggctttt
    22441 ggctatttag cccgtgcctt ccagcatgaa atcgatcact tagacggtat cttgttcatt
    22501 gagaaaatga tacagcagat cccggaagaa gaacttgaag aatatatgga ggaacatctt
    22561 gatgactaag ctagtattta tgggcacacc tgctttttct gtgccgattc ttgaagggct
    22621 gatagaatca ggttacgaga tccaggcagt ggtaacacag cccgaccgtc cagttgggcg
    22681 gaaacgagta attacgccga caccggtaaa aaaagcggct ctgaagcacg gtatccgtgt
    22741 gctgcagcca gagaaaatct ccggttcgcc agaaatggaa gaaatcattg aattggtgcc
    22801 tgatgtgatc gtcactgcgg catttggcca attcttgcct gaaaagttat tgcaagtacc
    22861 aaaattaggg gcaatcaatg tccatgcttc tttacttcct aaatatcggg gaggtgcgcc
    22921 tgttcattat gccatcatga atggggagaa agaaactggt gtaacgatca tggaaatgat
    22981 caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc ataccaatca ctaaacaaga
    23041 tgatgtaggg acgatgtttg aaaagcttag ccttttagga agaaagctat tgctagaaac
    23101 attgcccaat attttggatg gacaaaaacc agtacctcaa gacgagtcag aagcaacttt
    23161 ttcgccaaac atcacacggg aacaagaagc aatcgattgg cataaaactg cagaagaaat
    23221 tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca ttcacgacct atgagggcat
    23281 acgctggaaa ttattatcgg ttgaagtatt ggcagaaact acagacaaag cacctggtac
    23341 gatcatccgg aaagacaaaa aaaatctatg gattgcttgc ggtaaacaga ccgtcttagc
    23401 catcaatgaa ttacagccag ctggtaaagg aaaacaggcc gtacatgatt tccttaacgg
    23461 aagcggtcag caagttcaga ttggtcagca ggtgaaataa tgaaaaaaat ccctaaaaaa
    23521 atgaaaaaat cagtacgata tatggcatta ctgacattag agcgagtgaa caagggtggc
    23581 gcctactcga atcttttgtt gaatgagatg atcaaacaaa gcgagctaaa tcaaaaagat
    23641 gtgggtttat ttacagaatt gatctatggt acgattagcc atcagcgact attggaattt
    23701 tacgttgcac cattgatcgc taaagcaaaa aaagtggatg actgggtcaa gatgcttctt
    23761 tatctttctg tataccaatt agaattttta gataaagtcc ctgcacatgc tattttaaac
    23821 gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga cagggaaatt tgtaaatggt
    23881 gtcttgagga attatcagcg tcaaggagca ccagatctgc aaggcattgc tgatcctatc
    23941 gaaagacttt ctgtcaaaat cagcctgcct ctatggatga cgaaacgact ggtggatcaa
    24001 atcggatacg aagaaactga aaagctcggt ttatcgctat atcagcctag ccatgctagt
    24061 gcccgagtag atacacgccg actttccaga gatcaggcca tcactgtttt gcaagaagaa
    24121 gagatcacag ctgaaaaaag ccaagtcagt ccatacggta tagtagcgaa aaaagggcat
    24181 ttagcttcta gctcattgtt ccacgatggc gtcatgacga tccaagatga aagttcgatg
    24241 cttgtagcgc cgtctatgca aatcgagcct gaacatcatg tcttagatgc ctgtgcagca
    24301 cccggaggta agacgacgca tattgcttct tttttagatc cgtcatgcgg tggagaagtg
    24361 gtcgctttag atgtacacga acacaaagtc aaactcattg aagataatgc agcacgtcta
    24421 ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta gagaagtcag taaacagttc
    24481 gaagcagagc agtttgatcg gattttagta gacgcgcctt gttcaggtct tgggctgctt
    24541 cggcgaaaac cagatattcg ttataaaaaa cagccggatg aattagcgaa tttaccaaaa
    24601 atccaattgg aaattttaga aagctgcgca ccaactttaa agtcttccgg tattctgact
    24661 tatagcactt gtacgatctt aaaagaagaa aaccaagacg ttgtttctgc tttcttggaa
    24721 agacaccctg attttgagag gattgatgtt ttgaccaatc ctatgctaca atcatcaatc
    24781 gaagagaaaa tgttgacttt gtatccgcat cagttttaca cagatggatt tttcatttgt
    24841 tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg aatatcaatc agatgtagga
    24901 agacgacgta acacaaatca agactatgcc agtgtattta ccagccgttc aggtctcaag
    24961 cttgctgtat tagcagacgg gatgggtggt catcgtgccg gagacattgc cagtcagatg
    25021 gcggtaacga atctaggcga agcttgggaa aaacaagaag ctgttgacga tgaaaaaatc
    25081 gcccaatggt tcattcaatc gattcaagaa gaaaatatgc tgatttatca gcgtggacaa
    25141 gaacagccgg aatataacgg tatgggaaca acaatcgtag cggccgctct gtcagatgac
    25201 tggttcacga ttgccaatgt tggggatagc agagcttatt tgattcgtga agataaaatc
    25261 atgcaattga cggaggatca ttctttagtc aatgaactag tcaaatcagg agaaatcagc
    25321 gaagaaatgg cagtgaacca tccaaggaaa aatattttaa cacgttctgt cggcatgcct
    25381 ggaacagtcg aagtagatgt ttctactcat ctttggcaac taaaagatcg actgctgtta
    25441 tgttcagatg gcttgacgaa catgctgtct gaagaagaaa taggaacgat catcaatcaa
    25501 gaaggcccat tgtctcataa agtaactgag ctgattgatc gtgccaatga agcaggcgga
    25561 gcggataata tcaccgttct tcttatcgaa tataaggagg acagtgtatg atcgagttgg
    25621 gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat cggtagcggt ggtatggcaa
    25681 atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt agcagttaaa gtcttacgat
    25741 ttgactttca aaatgaccag actgcgattc gacgatttca gagagaagct ttagcagcaa
    25801 cagaactcgt tcatccaaac attgttagtg tttacgatgt tggtgaagaa gacaacatgc
    25861 agtatctggt gatggaatac gtcaaaggaa tggacttgaa acgatatatc caaacacatt
    25921 atcctgttcc ttatgaaaca gcagtaaata ttatgcagca gatcctttct gctatttctt
    25981 tagctcataa ccatcaaatc atccaccgag atttgaaacc ccaaaacgta ctgatcgata
    26041 atgaaggtgt tgtaaaaatc acggatttcg ggattgctat cgctttatct gaaacatcga
    26101 tcacgcagac gaatacgatg ctaggatctg ttcattatct gtcccctgag caggccagag
    26161 gaagtatggc aacgaagcag tcagatattt atgctttagg gatcatcctt tatgagatgt
    26221 tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac aattgcttta aaacattttc
    26281 aagatgatct tccatctata aaagcacttg atccaaatgt gccacaagca ctggaaaacg
    26341 tgattttacg ggcaacggct aaagaacctg cagaccgtta taagtcagcc gaggaaatgt
    26401 ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa cgaagaaaaa tggcagcctc
    26461 atgtgatgga taatgaaaca aaagttatca caccccttac ggaagatgca ccaatgcctg
    26521 attcattcaa atctatgcct ctgccaaaag acaagccaga aaacgaaacc ttaccagagg
    26581 aggaagaagt tctgcctgat cctaaaaaga aaggacagaa aaaatggtgg ctgatcctat
    26641 tagctattct tgctgtttta ggaatcggaa cagctgtatt ctttgcttct ggcgggcgag
    26701 gagaagttgt tgttcctgat gtttctgatc tttctgaagc ttctgctaga gaaacactcg
    26761 caaaagcgaa attagaagta gcaacaaaaa cagaagaaat tgcagatgac aaaatcgaag
    26821 aaggtaatgt agtgaagaca gacccggcag cgggaacgac agtcaagcaa aaacgcgaag
    26881 tcactctgta tattagtagc ggcacgaaaa aaatcaaatt agatgattac agtggtaaaa
    26941 gttatgaaga tgccgaagca agtttgaaag aattagggtt ttctgctgac ttgattcaaa
    27001 aagaagagga attcagttca gaggtagaaa gcggaatgat catttcgcaa tctccatcag
    27061 aagatacaga agtcgatcct aaaacagaca cgattacttt cacagtcagc aaaggaccag
    27121 aaacagtgac tttgagtagt catgtaggag aaaactacga aactgttcgt aattggcttg
    27181 ttacacaagg attcaatatc aataatatta gtgctactta tgactatagc gacactgtac
    27241 catcaggttc gatcatcagt caggatccag cctcgggtga agttgtagca gaagaaactt
    27301 acatcaattt tgtcgtaagc caaggaacag agcctaaatt gaatgatatc tccggctata
    27361 caaaaagcga agcacaaagt tatttagcta gtgtcggtgc agaatatatc gggcatgaga
    27421 cttccgaata ttcaaacacg gtggataaag ataaagtgat tcgtacagta ccaggggcag
    27481 gaacgacaat taccaaagga atggttgtga acgtaattta ctctaaagga ccagatccag
    27541 cgtcttctag ttctgaaaaa gactcttcag acagttctag cagctcaagt agctccagta
    27601 gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac aagttcatca gctagcacct
    27661 cagaaagttc aaaataagtg ctgaataaaa tatatcagaa gggaatgcga cattgatttg
    27721 ttgcattctc ttttttgtgt ttctatctct tttgatcaat tttcaagcca tatactttga
    27781 gaagtttgca tggaataaca cctactttcc tcttcgtatg gtaaaataaa caaaaacgga
    27841 ggtggcactt atcaaaggac aaatcagaaa agcaatcagc ggtttttact atgtatacgc
    27901 aaacggagag acctatcaaa caagaggaag aggaaatttc cggaatcgga aaatcacgcc
    27961 gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa acagacggct atcttctgga
    28021 aatcttgccg agaaaaaatc aattagttcg cccaccagtc acaaatgttg atcttggaat
    28081 cattgtcaca agtatcgtag agcctgcatt ttcttataat ctacttgacc gctttctagt
    28141 taccttggaa tacgagtcga tcgaaccgat tatttttttg acaaagaccg acttgtcaga
    28201 cgacccagaa aaaatcgaag agatcaagca aacctatact aaaataggct atccggtgat
    28261 tgtacccaat catacgggtg atacgacaga actgatccgt tacttcccag aacggctatc
    28321 ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta ctgaatcaaa tatcaccgga
    28381 actgaattta gcaacaggag agatttctga tgcacttgga agaggacggc atacaacacg
    28441 tcatgtggaa ctgattccta tctatggcgg gttagtagca gatacaccag gatttagctc
    28501 gattgacttt ttgacaattg aagcaggaga actgccgaga caatttcctg aatttgtaga
    28561 agcttctgtc cattgcaaat ttagagagtg catgcatgct aaagaacctg gctgcgaagt
    28621 aaaacataga gtagaaacag gtggtatcgc acagacacgt tacgacaatt atttgcaatt
    28681 cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa aaagaaggta aacgaaaata
    28741 ggaggaaaca agaatgaaga tagcaccatc cattttgagt gcagattttg ccaatttaca
    28801 aagagacatt gagttagtgg aaaaaggagg cgcagattac atccacgtag atgtaatgga
    28861 tggccgattt gtcccaaata tcacattagg gccaaatatt gttcaggcaa tccgtcctgt
    28921 tactaaatta ccgttagacg tccatctgat gatcgttgat ccagaaaact acattccagc
    28981 atttgcaaaa gctggagcag atatcattac tgtccacgtc gaagcaactc ctcatattca
    29041 tcgggcactt caaatgacaa aaagcttagg agttaaagct ggagtagtta tcaatccagg
    29101 aacaccagtc tccatgatca aacatgtttt gcctttggct gatcaagtac tagtcatgac
    29161 tgtcaatcct gggttcggcg gacaaagctt tattgaagaa acagtagaaa aaattacgga
    29221 actcgctgat ctacgtagag agaacaattg gacttatgca atcgaagtag atggcggaat
    29281 cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg gatgtcttcg ttgcaggatc
    29341 atatatctat ggagccgaaa atccagtcgg acaaatcgaa cgattaaaag aggcattaga
    29401 ctaaatgaaa attttattag ctgctggagg taatccgcaa aactggccgc agtctctgct
    29461 gtccaaatac gatgcctatg tcgggataga ccgtggtgcg ctgtacctgc ttcaagcagg
    29521 ctacccagta gatctggcag tgggtgattt tgattcattg tcgaacgaag agcagaagga
    29581 agtatttgca aaagccaaag agtatacccg ttcccctgca gaaaaagacg atacagatac
    29641 acagttaggt atcttaaaaa catttgaacg ttacccgcaa gcggaagtca ctttgattgg
    29701 agctacgggc ggaagattgg atcatctttt agccaatctc tggttaggta tggaacctcg
    29761 cttcaaaccc tttgcccatc agcttattat cagagattgt caaaattatt tgtcttatta
    29821 tttgcctgga gagcatatag taagacagat accagatatg aagtatttag cttattgctg
    29881 cttgactccc gtctcgaatc ttacattaaa aggcagcaag tatgtattgg aaaataaaga
    29941 agtagaatat ccgacatctt atgcaagtaa cgaatttctt tcagataccg cagaattttc
    30001 tttttcagat ggtatgattg cagttattca aagcaaagat tgatccataa acaaataaaa
    30061 aaatttgtct ttcaggctaa aaatgacaga atctattatt tgctaaaaaa aagcttaaga
    30121 ggagcaactt accttttgtt tcacggcatt tagtgataaa attgaacaca agagtctaat
    30181 taattcgagg tgtctttgtg aagaagttca atcctaataa aaatattatt attaccttgg
    30241 ttattgttat catcgtggtg acgatactaa gtgtgaccgc tgcgcagcga gcgaatgaag
    30301 gaaagcccaa tttcttccaa tccatcgtta atgacagtgt tgcaatggtt gataaaacaa
    30361 tctctgcccc agtacggtgg gtacgaaatg gagtagattc tgtccataat cttttttcaa
    30421 cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag ctatgatgac gtttcacagc
    30481 aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa agaacttgag ttgaatcaga
    30541 cgctgactag ttacgaaaaa gtaacggcaa atgtcattac tcgttcgcct gacagttggc
    30601 aggacatgct gatcgtagac aagggttcta aagacggtat cgaagcaaat atggcagtca
    30661 tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa tacggcttcg tctaaaatcg
    30721 aattactgtc atcctctaat gaaagctcca atcattttcc agtacgggta tcttcggcta
    30781 atggcgaagc gtttggtttg cttaaaaact atgatgaaaa gctccatgcc ttagtggtga
    30841 cccaattaac tggtgatacg gatatcaaag aaggggatgt tgtccagaca tccggtcttg
    30901 gagggaattc tccagctaac ttgccgatcg gtacggttat taaaacgaaa ccagatagtt
    30961 atgggctgga tcgggaagtt tatgtgaaac cttatgcaga aatgtatgac gtgtcagttg
    31021 tgacgattgt ccaacggcta gcggaggatt agtccatgtt gaagaaagaa acaatgaagt
    31081 actatctgcc aatcgttttg ttctttttga tgttgataga tggtcattta acaagaatgc
    31141 taggggagtg gtcgaaaggc acctatatgt caaatgccca ctttctgata ttggcattat
    31201 tatgttgcag tatggcgttt gaaaaacgtt atttactgat taccacgatt gttctcgggg
    31261 ctatctatga cgcttactat attggcgtta tcggtatcta tgcagtagct ctccctttaa
    31321 ttgtatggtt gatgtatgta atgaaagacg ttatccatgt caacatcttt actgaatttt
    31381 tcagtatgat catctttgtc acgggttatg aattgtttac gatggtggtc cagttgattt
    31441 ttaaattagc agtagtaaat aacacgtatt ttattacaag gtttttagga cctacactgc
    31501 tgttgaacat gattatattt gtattattca tttttccctt taagaaatta ttcagtgatg
    31561 aataatttct ttttttattt ttaggtcgaa agcgaaaaag aatcgttgtt atcttaagaa
    31621 atgctagact taacgcggct ttttcatgat cattttctga aacgaacagt tttgtcattt
    31681 ttaaaagttg ttttgaaaca tttttgtaac ataactatta tatcgttgac acactgctat
    31741 ggtacaattt ttatatcgtt aagaaagact taaatatatt tagatattta ttaaattaag
    31801 ccagtcggag gaatgaagag tgaaaaagag tttaatatca gcagtaatgg tatgttcaat
    31861 gaccttgact gccgtagcat caccgatcgc tgccgcggcg gacgattttg attctcagat
    31921 acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa caagcagatg cacaatcaca
    31981 aatcgatgcc ttagaaagcc aagtttctga aatcaacaca caagcacaag acctattagc
    32041 aaaacaagat acattgcgtc aagaatctgc acaattagta aaagacattg cagatttaca
    32101 agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa gcacgcgaag cacaagtaag
    32161 caacacaagc tcaaactaca tcgatgctgt attgaatgct gattcattag cagatgctat
    32221 tggacgcgtt caagcaatga caacgatggt aaaagcaaac aacgacttga tggaacaaca
    32281 aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa aacgatgcta aactaaaaga
    32341 attagctgaa aaccaagctg ctttggaatc tcaaaaaggc gatcttttat ctaaacaagc
    32401 tgatttgaac gtactgaaaa cttcattagc tgctgaacaa gcaactgctg aagacaagaa
    32461 agctgacttg aaccgtcaaa aagcagaagc tgaagcagag caagctcgta ttcgtgaaca
    32521 acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa gaaaaagctg aaaaagaagc
    32581 acgcgaacaa gcagaggctg aagcacaagc tactcaagct tcatctactg cacaaagctc
    32641 agcaacagaa gaatcatcag ctacacaatc ttcaatgact gaagaatcat catcagctac
    32701 acaatcttca gcaacagaag aatcaacaac acctgaatct tcaacagaag aatcaacagc
    32761 gcctgaatct tcagcaacag aagaatcaac aacag
//
LOCUS       DASJRQ010000021        32335 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJRQ010000021 DASJRQ010000000
VERSION     DASJRQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.021"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..550
                     /gene="nrdI"
                     /locus_tag="U4A80_000920"
     CDS             104..550
                     /gene="nrdI"
                     /locus_tag="U4A80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1974553.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(615..2597)
                     /locus_tag="U4A80_000921"
     CDS             complement(615..2597)
                     /locus_tag="U4A80_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1974554.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2611..3507)
                     /gene="murQ"
                     /locus_tag="U4A80_000922"
     CDS             complement(2611..3507)
                     /gene="murQ"
                     /locus_tag="U4A80_000922"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1974555.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3510..4571)
                     /locus_tag="U4A80_000923"
     CDS             complement(3510..4571)
                     /locus_tag="U4A80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1974556.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4782..5348)
                     /locus_tag="U4A80_000924"
     CDS             complement(4782..5348)
                     /locus_tag="U4A80_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1974557.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(5376..6878)
                     /gene="eat(A)"
                     /locus_tag="U4A80_000925"
     CDS             complement(5376..6878)
                     /gene="eat(A)"
                     /locus_tag="U4A80_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1974558.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7136..8257)
                     /locus_tag="U4A80_000926"
     CDS             complement(7136..8257)
                     /locus_tag="U4A80_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1974559.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8383..9324
                     /locus_tag="U4A80_000927"
     CDS             8383..9324
                     /locus_tag="U4A80_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1974560.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9474..10370)
                     /locus_tag="U4A80_000928"
     CDS             complement(9474..10370)
                     /locus_tag="U4A80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1974561.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10639..11979
                     /locus_tag="U4A80_000929"
     CDS             10639..11979
                     /locus_tag="U4A80_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1974562.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12197..12640
                     /locus_tag="U4A80_000930"
     CDS             12197..12640
                     /locus_tag="U4A80_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1974563.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13144..14562)
                     /gene="nhaC"
                     /locus_tag="U4A80_000931"
     CDS             complement(13144..14562)
                     /gene="nhaC"
                     /locus_tag="U4A80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1974564.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14965..16188)
                     /locus_tag="U4A80_000932"
     CDS             complement(14965..16188)
                     /locus_tag="U4A80_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1974565.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16219..17058)
                     /locus_tag="U4A80_000933"
     CDS             complement(16219..17058)
                     /locus_tag="U4A80_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1974566.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17075..17755)
                     /locus_tag="U4A80_000934"
     CDS             complement(17075..17755)
                     /locus_tag="U4A80_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974567.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17757..18830)
                     /locus_tag="U4A80_000935"
     CDS             complement(17757..18830)
                     /locus_tag="U4A80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974568.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18886..19124)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19167..19400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19477..20148)
                     /locus_tag="U4A80_000936"
     CDS             complement(19477..20148)
                     /locus_tag="U4A80_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1974569.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20235..22913)
                     /gene="mgtA"
                     /locus_tag="U4A80_000937"
     CDS             complement(20235..22913)
                     /gene="mgtA"
                     /locus_tag="U4A80_000937"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1974570.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            23572..24009
                     /gene="ndk"
                     /locus_tag="U4A80_000938"
     CDS             23572..24009
                     /gene="ndk"
                     /locus_tag="U4A80_000938"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1974571.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24031..24897)
                     /locus_tag="U4A80_000939"
     CDS             complement(24031..24897)
                     /locus_tag="U4A80_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1974572.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25057..27150)
                     /locus_tag="U4A80_000940"
     CDS             complement(25057..27150)
                     /locus_tag="U4A80_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1974573.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27899..28522)
                     /locus_tag="U4A80_000941"
     CDS             complement(27899..28522)
                     /locus_tag="U4A80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974574.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28681..30078)
                     /locus_tag="U4A80_000942"
     CDS             complement(28681..30078)
                     /locus_tag="U4A80_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1974575.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30110..31081)
                     /locus_tag="U4A80_000943"
     CDS             complement(30110..31081)
                     /locus_tag="U4A80_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1974576.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31455..31880
                     /locus_tag="U4A80_000944"
     CDS             31455..31880
                     /locus_tag="U4A80_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974577.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         9247 a         6778 c         5636 g        10674 t
ORIGIN      
        1 ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac caaaaaactt
       61 aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca ttttatatat
      121 atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat
      181 gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg aaaatagtga
      241 ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag acggcggaaa
      301 tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat atctggaaca
      361 tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa acttcaataa
      421 tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt tcttggcaga
      481 ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt tagcagaaaa
      541 caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct ttcgtttcta
      601 ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt tgctttacct
      661 gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac gatcatggat
      721 gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag cgtatcaggt
      781 gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa gtcaactgtg
      841 ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt
      901 ttcgttggga agacagagct gactacccca gtaactggag aatagatatg tccgttttct
      961 ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc acttgcttta
     1021 tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact ttcagctgaa
     1081 gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt cgctaattct
     1141 tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa gccaccagca
     1201 taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat caatgggatc
     1261 aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat tccgatcact
     1321 gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag aggtaatgtt
     1381 actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc tcctttgatc
     1441 atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc tgcacccact
     1501 tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt ttcatcaatc
     1561 atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat cggcaagaat
     1621 aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca gttgatccca
     1681 ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga
     1741 ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc aggtactact
     1801 ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac acctaggatc
     1861 ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg tgcttccgga
     1921 ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg tgttgcacca
     1981 aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat cccgtttttc
     2041 aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt tccggctgtg
     2101 actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac aaatgcagga
     2161 atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt agatggtttt
     2221 tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg gtcttttcca
     2281 gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc taatttcacg
     2341 cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc gatgatgact
     2401 tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa ttcagagaca
     2461 tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg gacaatgctt
     2521 tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc ttccaaagtc
     2581 agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa atccgtttgc
     2641 ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca tgacgatagc
     2701 cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt aatcagcttc
     2761 tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg ttggtttgac
     2821 atcgaccatc aagttattga atactttacc tataccaatc atactgatag tagaaatcat
     2881 attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg tcaaaaattc
     2941 tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt
     3001 gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc caccgatcac
     3061 atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg tcaaattcag
     3121 cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga cagctacagt
     3181 cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta caccgaatgt
     3241 cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg ctcccatata
     3301 aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg gttcgatact
     3361 tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca ttagttgaac
     3421 ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc gttccgttgt
     3481 taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat aaattgatag
     3541 ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac tttagctact
     3601 acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc acccatatag
     3661 cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc tgtatttctt
     3721 gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca
     3781 tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc atctacaggt
     3841 tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc ctgaacgttt
     3901 tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa tagatctaga
     3961 gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt
     4021 gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc ttgaattttg
     4081 agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg acgtggataa
     4141 taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata
     4201 tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga ccagtcggca
     4261 atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat tgctttcaaa
     4321 ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga aatatcgacc
     4381 atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg cttttgatat
     4441 gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa gccattggat
     4501 gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat aaatattgaa
     4561 aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga ctagagtata
     4621 tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca tttctaaagg
     4681 ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct aagcaaaagc
     4741 agtgccttga aatattttat ttaaataaca caaaaacata attactcata agaaaccaat
     4801 tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca
     4861 ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg
     4921 atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt
     4981 gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt
     5041 ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg
     5101 agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt
     5161 ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt
     5221 cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt
     5281 gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc
     5341 ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc
     5401 tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc
     5461 acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt
     5521 tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc
     5581 cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc
     5641 tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc
     5701 aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc
     5761 tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat
     5821 gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa
     5881 tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt
     5941 cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt
     6001 aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt
     6061 ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta
     6121 tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct
     6181 ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc
     6241 atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa
     6301 agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc
     6361 cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt
     6421 tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca
     6481 tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat
     6541 tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat
     6601 atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc
     6661 tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg
     6721 ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt
     6781 ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg
     6841 tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa
     6901 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag
     6961 acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc
     7021 acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc
     7081 ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact
     7141 tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg
     7201 tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa
     7261 ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta
     7321 ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg
     7381 tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt
     7441 ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa
     7501 aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct
     7561 cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg
     7621 tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat
     7681 atggattggt aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc
     7741 gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag
     7801 aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag
     7861 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
     7921 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
     7981 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
     8041 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
     8101 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
     8161 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
     8221 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
     8281 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
     8341 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
     8401 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
     8461 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
     8521 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
     8581 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
     8641 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
     8701 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
     8761 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
     8821 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
     8881 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
     8941 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
     9001 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
     9061 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
     9121 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
     9181 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
     9241 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
     9301 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
     9361 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
     9421 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
     9481 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
     9541 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
     9601 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
     9661 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
     9721 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
     9781 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
     9841 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
     9901 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
     9961 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
    10021 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    10081 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    10141 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
    10201 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    10261 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    10321 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    10381 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    10441 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    10501 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
    10561 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    10621 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    10681 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    10741 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
    10801 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    10861 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    10921 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    10981 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    11041 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    11101 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
    11161 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    11221 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    11281 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    11341 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    11401 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
    11461 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
    11521 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    11581 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    11641 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    11701 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    11761 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    11821 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    11881 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    11941 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    12001 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
    12061 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    12121 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    12181 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    12241 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    12301 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    12361 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    12421 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    12481 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
    12541 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    12601 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    12661 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
    12721 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    12781 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    12841 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
    12901 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
    12961 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
    13021 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
    13081 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
    13141 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
    13201 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
    13261 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
    13321 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
    13381 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
    13441 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
    13501 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
    13561 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
    13621 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
    13681 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
    13741 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
    13801 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
    13861 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
    13921 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
    13981 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
    14041 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
    14101 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
    14161 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
    14221 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
    14281 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    14341 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
    14401 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
    14461 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
    14521 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
    14581 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
    14641 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
    14701 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
    14761 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    14821 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
    14881 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    14941 tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
    15001 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
    15061 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
    15121 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
    15181 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
    15241 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
    15301 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
    15361 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
    15421 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
    15481 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
    15541 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
    15601 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
    15661 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
    15721 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
    15781 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
    15841 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
    15901 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
    15961 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
    16021 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
    16081 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
    16141 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
    16201 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
    16261 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
    16321 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
    16381 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
    16441 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
    16501 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
    16561 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
    16621 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
    16681 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
    16741 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
    16801 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
    16861 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
    16921 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
    16981 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
    17041 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
    17101 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
    17161 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
    17221 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
    17281 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
    17341 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
    17401 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
    17461 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
    17521 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
    17581 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
    17641 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
    17701 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
    17761 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
    17821 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    17881 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    17941 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    18001 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    18061 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    18121 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    18181 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    18241 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    18301 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    18361 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    18421 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    18481 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    18541 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    18601 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    18661 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    18721 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    18781 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    18841 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    18901 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    18961 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    19021 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    19081 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    19141 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    19201 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    19261 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    19321 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    19381 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    19441 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    19501 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    19561 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    19621 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    19681 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    19741 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    19801 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    19861 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    19921 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    19981 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    20041 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    20101 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    20161 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    20221 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    20281 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    20341 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    20401 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    20461 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    20521 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    20581 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    20641 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    20701 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    20761 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    20821 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    20881 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    20941 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    21001 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    21061 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    21121 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    21181 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    21241 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    21301 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    21361 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    21421 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    21481 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    21541 gaaatgttca gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga
    21601 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    21661 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    21721 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    21781 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    21841 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    21901 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    21961 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    22021 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    22081 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    22141 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    22201 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    22261 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    22321 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    22381 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    22441 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    22501 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    22561 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    22621 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    22681 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    22741 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    22801 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    22861 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    22921 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    22981 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    23041 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    23101 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    23161 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    23221 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    23281 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    23341 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    23401 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    23461 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    23521 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    23581 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    23641 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    23701 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    23761 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    23821 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    23881 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    23941 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    24001 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    24061 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    24121 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    24181 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    24241 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    24301 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    24361 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    24421 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    24481 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    24541 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    24601 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    24661 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    24721 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    24781 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    24841 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    24901 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    24961 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    25021 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    25081 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    25141 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    25201 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    25261 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    25321 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    25381 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    25441 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    25501 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    25561 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    25621 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    25681 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    25741 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    25801 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    25861 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    25921 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    25981 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    26041 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    26101 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    26161 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    26221 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    26281 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    26341 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    26401 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    26461 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    26521 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    26581 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    26641 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    26701 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    26761 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    26821 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    26881 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    26941 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    27001 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    27061 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    27121 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    27181 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    27241 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    27301 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    27361 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    27421 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    27481 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    27541 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    27601 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    27661 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    27721 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    27781 ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    27841 tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc
    27901 attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat
    27961 tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg
    28021 actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt
    28081 ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt
    28141 ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat
    28201 gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa
    28261 taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat
    28321 tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta
    28381 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    28441 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    28501 gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt
    28561 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    28621 tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc
    28681 ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat
    28741 tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc
    28801 attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc
    28861 ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc
    28921 atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt
    28981 ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata
    29041 gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc
    29101 ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt
    29161 tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt
    29221 gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc
    29281 aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa
    29341 cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct
    29401 tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt
    29461 gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc
    29521 tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc
    29581 attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata
    29641 tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat
    29701 cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc
    29761 aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat
    29821 caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg
    29881 tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa
    29941 gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc
    30001 tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg
    30061 aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc
    30121 catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc
    30181 gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga
    30241 ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat
    30301 ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt
    30361 aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc
    30421 cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga
    30481 ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa
    30541 taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt
    30601 ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga
    30661 taactggaat actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga
    30721 agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt
    30781 ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca gctactcttt
    30841 cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa
    30901 tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg
    30961 ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt
    31021 aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca
    31081 tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga
    31141 ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag
    31201 agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa
    31261 tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat
    31321 tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt
    31381 tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa
    31441 atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga
    31501 cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt
    31561 cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac
    31621 gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga
    31681 cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc
    31741 tgacagatat caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata
    31801 aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg
    31861 gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt
    31921 ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct
    31981 gattattgta catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc
    32041 ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct
    32101 tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac
    32161 tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta
    32221 aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa
    32281 tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg tagta
//
LOCUS       DASJRQ010000022        30762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJRQ010000022 DASJRQ010000000
VERSION     DASJRQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.022"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(153..2228)
                     /locus_tag="U4A80_000945"
     CDS             complement(153..2228)
                     /locus_tag="U4A80_000945"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1974578.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="U4A80_000946"
     CDS             complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="U4A80_000946"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1974579.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3531..4343)
                     /gene="recO"
                     /locus_tag="U4A80_000947"
     CDS             complement(3531..4343)
                     /gene="recO"
                     /locus_tag="U4A80_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1974580.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4486..5385)
                     /gene="era"
                     /locus_tag="U4A80_000948"
     CDS             complement(4486..5385)
                     /gene="era"
                     /locus_tag="U4A80_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1974581.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5400..5795)
                     /locus_tag="U4A80_000949"
     CDS             complement(5400..5795)
                     /locus_tag="U4A80_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1974582.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5779..6255)
                     /gene="ybeY"
                     /locus_tag="U4A80_000950"
     CDS             complement(5779..6255)
                     /gene="ybeY"
                     /locus_tag="U4A80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1974583.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6272..8464)
                     /locus_tag="U4A80_000951"
     CDS             complement(6272..8464)
                     /locus_tag="U4A80_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1974584.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8488..9459)
                     /locus_tag="U4A80_000952"
     CDS             complement(8488..9459)
                     /locus_tag="U4A80_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1974585.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10165..11544
                     /gene="brnQ"
                     /locus_tag="U4A80_000953"
     CDS             10165..11544
                     /gene="brnQ"
                     /locus_tag="U4A80_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1974586.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11700..12146)
                     /locus_tag="U4A80_000954"
     CDS             complement(11700..12146)
                     /locus_tag="U4A80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1974587.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12173..12349)
                     /locus_tag="U4A80_000955"
     CDS             complement(12173..12349)
                     /locus_tag="U4A80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1974588.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12511..12939)
                     /locus_tag="U4A80_000956"
     CDS             complement(12511..12939)
                     /locus_tag="U4A80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1974589.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13031..13891
                     /locus_tag="U4A80_000957"
     CDS             13031..13891
                     /locus_tag="U4A80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1974590.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(13975..14325)
                     /locus_tag="U4A80_000958"
     CDS             complement(13975..14325)
                     /locus_tag="U4A80_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1974591.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14341..16068)
                     /locus_tag="U4A80_000959"
     CDS             complement(14341..16068)
                     /locus_tag="U4A80_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1974592.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16281..17207)
                     /gene="trxB"
                     /locus_tag="U4A80_000960"
     CDS             complement(16281..17207)
                     /gene="trxB"
                     /locus_tag="U4A80_000960"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1974593.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17352..18788)
                     /locus_tag="U4A80_000961"
     CDS             complement(17352..18788)
                     /locus_tag="U4A80_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1974594.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18785..20188)
                     /locus_tag="U4A80_000962"
     CDS             complement(18785..20188)
                     /locus_tag="U4A80_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1974595.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20418..21320
                     /locus_tag="U4A80_000963"
     CDS             20418..21320
                     /locus_tag="U4A80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1974596.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21361..23247)
                     /locus_tag="U4A80_000964"
     CDS             complement(21361..23247)
                     /locus_tag="U4A80_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1974597.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23344..24195)
                     /locus_tag="U4A80_000965"
     CDS             complement(23344..24195)
                     /locus_tag="U4A80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1974598.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24653..26437)
                     /locus_tag="U4A80_000966"
     CDS             complement(24653..26437)
                     /locus_tag="U4A80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974599.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26437..28188)
                     /locus_tag="U4A80_000967"
     CDS             complement(26437..28188)
                     /locus_tag="U4A80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974600.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28330..28554)
                     /locus_tag="U4A80_000968"
     CDS             complement(28330..28554)
                     /locus_tag="U4A80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1974601.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28805..30391
                     /locus_tag="U4A80_000969"
     CDS             28805..30391
                     /locus_tag="U4A80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1974602.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8578 a         6621 c         5492 g        10071 t
ORIGIN      
        1 attcagttga aattgaccga ctcaaattac ttgtataata cctaggaaaa aattaactag
       61 caccacgggt atagttattt atatttataa caaataaacc agagaaatag cctattttgt
      121 gcctattctc tggtttattt acatattaga aattattttg tgatgatttg atctaaactt
      181 gctagcgaca aagccatggc agcaatttga cttaattgcg ttaatcggtt cttacgtact
      241 gctggttctt ctgccatgac catcgtctga tcaaaataat tttcgatcag tgggcgaagg
      301 cttacaagct gatcataatt ttcgttgatc gtttggtcag caaagctttt tttcattgcc
      361 tctactgctt tgtgcagttc tttctccgct tcattttcga acagttcagg gtctacttct
      421 gattgttttt caagctccgc ttttttcgct aagttcacga cacgtgtcaa agcttccatc
      481 gatggtttga agtcttcatc tgcaagatgc tgcttgaaca attgtgctgc tgcaaatact
      541 tttgtcagat ctttttgttc tgcattcaaa acagcttcga tgacatcatg acgaatattc
      601 ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacctg ttgttgccct
      661 tctgataaac caatgccgaa acgatccgta tctttactga taacatcact aatcgtttct
      721 tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgatccgaac gacaccgtat
      781 gcttggcgac gtaaggcata aggatcgttt gacccgcttg gaatcatccc aactgcaaag
      841 aaagaaaaaa tgctatctaa tttatctgca atcgctaaaa cagaacctac tgtagactga
      901 ggtagctctc cttcactgga tataggcatg taatgctcac gaatcgctgt tgctacagca
      961 ggtttctcgc ctttcagcag agcatatttt tcgcccatga tcccttgtag ttcaggaaat
     1021 tcccctacca tgttggttac tagatcaaat ttatagattt cagcagctcg ttgcagatcc
     1081 atcaattctt cctcagatag tcctacttgt tcgccaatga tacgggcaat gacttgtacc
     1141 cgctgcattt tctcatagat actaccgatt ttttcatgga aagtaacagt ttttagtttt
     1201 tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc cgcatcttct
     1261 aaacgagccg tcaatacttt ttgattgcct cgaaccacat tttccaattt tacccgatca
     1321 ccattacgag cagaaataaa atgaggaagc agcatacctt gatcgttccg tacctcgaaa
     1381 taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga aagatatttt
     1441 tcttcgaagt cgccagaaaa gacagttgga tattcgacta aattgttgac ttcttctagt
     1501 aaatcttcgt ctagatcaat aacccagtga tttttttcgg ctaattcatt tgcttgtgta
     1561 acgatcattt cttgtctttt ttgcggtgaa gcaataacga attgttcatt caatttttct
     1621 tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg tcctcgagag
     1681 gctctgcctg ttttgacatc taagatctca aacgggatta cttcgtcatc taacaatgcc
     1741 acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg catcgttact
     1801 gggaaagtga ggcttttaat gacatctgct aaacctgtca atacttcttt cgcttctttg
     1861 ccgtgagtgt atttcgttac atagacatat tccacgcctt ttaattctct aaaagtgata
     1921 gcatctgtcg tcagaccttg tccgcggaca aatccttcag ctgctttcgt ccagtttcca
     1981 tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact gtctgcttga
     2041 cgttctggaa tgtttgttac tttgattgct aagcggcgcg gtgtagaaaa cgtttcgatc
     2101 gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc taattgtttc
     2161 atgcttggtg tgacaacatg agcaggcatt tcttccagcc cgatttcaag taataagtct
     2221 tttgccatct tattcagcct ccttcatgtt ttctggattt gctgtgtttt tattcaataa
     2281 agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac gagccatgtt
     2341 gcggatacgg gaaagataac ctgctcgttc tgttacggat actgcaccgc gagcatctaa
     2401 taaattgaat gtatggctgc atttcaaaat ataatcatac gctggatgaa caagattttc
     2461 atcgatacag cgtttagctt ctttttcaaa acggtcaaag ttgcttagta gcaagtcttg
     2521 gtcacttact tcaaaagaat atttggaatg ttcatattct ggttgtttga agatctcgcc
     2581 atatttcaca ccatcagccc attccagatc atagacactt tcaacttctt gaatatatga
     2641 agcaagacgt tctagtccat aagtgatctc agaagtaact ggtttacaag gtagtcctcc
     2701 gacttgctgg aagtacgtga attgtgtgat ctccatccca tcaagccata cttcccaacc
     2761 cacaccggca caccccatcg atgggttctc ccagttgtct tcaacaaaac gaatatcgtg
     2821 ttctaatgga tcaattccta aaagacgcaa gctttctaaa taaagctctt ggatattttc
     2881 aggtgaaggt ttcattacga cttgaaattg atgatgctga tacaaacggt ttgggttttc
     2941 tccataacgc ccgtcagctg gtcggcggga tggctctaca taagcagcat tccatggttc
     3001 cggtccgatt gcccgtaaga atgtatatgg gctcatcgta cctgctcctt tttctgtgtc
     3061 ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat
     3121 atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaattttt aagcgttgct
     3181 gtcaataaga atacaggaaa gaaaacgaca aaaagtccct atgtctgatt acagacatag
     3241 ggacgaataa tttcgcggtt ccaccctact tccgaataaa gattcggcag cttcatttat
     3301 cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg cttccactct cccgaactcg
     3361 ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg cagttttctt
     3421 gtttatataa tagatcctct cagtgaacga tctatttaat ctacggttaa taatagacgg
     3481 aagtccctgt atttgtcaaa tagttttgat ttttttgttg tatctttcat ttattcttct
     3541 ttttcaggta tctctttttc aggcatattt ttctctggcg tctttaatac gtgtgcccaa
     3601 gacttcattt gatcaatgaa ctttttgctt tttagatgaa taccgacata ttcatcatac
     3661 aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt ctttcaagtg gattccactg
     3721 atcttatcat aagaaatagc cgaaaacatt cgaacgaaat aaacagcgcg aggatctgca
     3781 tgataacggt gttcgtccat tggccagtgt ttctcgcata tcacaccgcc agatttggaa
     3841 gaatagtcaa attttccttt tgtttccccg catacgacac agtgcgtcca aattggcgcg
     3901 attccgaagc gttgcagtat ttgaacttca aagatattcg tgatgatttc tgcatcatct
     3961 ccttgatcca tccgctgcag agcttgttgc gtaaattgga ataaatgcgg gtcatattga
     4021 tgatcttcga ttgctgcatc tactagattt agcaaatagg taccataacc gctcaaaaag
     4081 atatcttctt ggatgttccg aaacgcatgg acgtctttga cactgcttaa aaacgacaaa
     4141 ccttcacttc gaaaattacc gatatagact gcttcggtaa aggggagcaa agccggagct
     4201 aatggctgat tttgccgatg gattcctttg atataaaaca tcattttgcc agaagattcc
     4261 gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa aatcaagcct
     4321 ttcgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg aaaagcagat
     4381 tctgccgatc cagattattt atgtttcgat cttccgccgt ttttctcaac gtgcggattt
     4441 ttatccagtt tatttctata cttaacctat aagaagttca gaatatcagt attcgtcttt
     4501 tcggtagcca aagtcttgca gatagacttt tttgtctcgc cagtcttttt ggactttgac
     4561 ccaaagttct agaaatactt tgtctcctaa aagattttcg atatcctttc ttgctttcgt
     4621 tcctacttgc ttcagcattt ttccgccttt gccgataatg attccttttt ggctatctct
     4681 ttcgacgatg atcgtggcct ggatatggat tttatcattc tcgtttcgct tcatcgaatc
     4741 aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg
     4801 gatcaattca gaaacgataa agtattcagg atgatcagtc acttgatcat ctggaaaata
     4861 ttgtgggccc tctggcattt cactgactaa tacatccatt aagcgttcga cattgtttcc
     4921 ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc tgcgtcgaat aatcttcgat
     4981 gattcccagc aactcgtctg gatggatctt atcgatttta ttgataatca gataaactgg
     5041 agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccggccgc gtttttgatc
     5101 agcactgatc ataaataaga ctgcatccac ttctttcaac gcactgtaag ctgcttctac
     5161 cataaaatct cctagacgat gctttggttt atggattcct ggtgtatcga taaaaatcag
     5221 ctgagcttct ggtgtagtat atactccttg gattttattt ctagttgtct gtgccttgtc
     5281 actcataatg gcaatttttt gacccacgat tcggttcaat aatgtggatt ttcctacatt
     5341 cgggcggccg acgattgcga caaaccctga tttatgttct ggcatatacg ttctcctttt
     5401 taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaat gatgacagca
     5461 aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga
     5521 tgaaaatgaa aatccgtaaa catgtccact acattttcaa aaacagtatt gatgatctct
     5581 actatccaaa cgagaaaaac agctaaaagc agccataacc actctatccg atcaagctgg
     5641 aaaatcacgc ccataaataa agcaaggata ccgaacgcaa catgtttcct catattgcgt
     5701 tcttcctcaa aaacggtttt gactccttgg acagcaaatt ccacagacat caggaaatgt
     5761 ttgtttttct ctacgttttt atcttttgag tccataggca tccaagatct ctttctgtaa
     5821 accaaacatt tctttctcat cttctggtgt catatggtca tacccgttga tatgcaagaa
     5881 tccatgtacc gctaaaaagc caagttcccg atcatatgaa tggccgtaag cttctgcttg
     5941 ttcagcagca cgttcaatag aaatcatgat gtctcctagt acacgaggca aaggattctc
     6001 tccatcttca aaaatcactg ggatttcgtc ttcgccttct tcttccagag caaaactgat
     6061 gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga ttgccgcatt
     6121 gtccatgaaa gtaacagaca tttctgtttc ctctggcagc tttaaataag atgctgcaaa
     6181 ttgcagcaag tcatcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat
     6241 aaaggtaata tccattatac atcgcttctt tctattcttc ttgttcttcc tttatcttct
     6301 caacttctgt tttcatttta ggatatttga tccgagaatg gaaggtgcta cttaaaccgc
     6361 tgatcaatga tttttccact tttcggattt ctttcaaggt cagtccacta tcgtctaatt
     6421 gaccatcgga aatacgctct tcgataagat tatggacaaa actttcgatt ttttcgctcg
     6481 tcggatgatc cattgcccgt actgcagcct cacaactgtc agcaatattg acgatccctg
     6541 cttcacgact ttgcggtttt ggtccaggat aacggaattg ctcttctgtt acttccggat
     6601 tgcgttcttt cgctttgatg tagaaataac gcattagcgt ggttccatga tgctgtttgc
     6661 aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggct ccatctgtca
     6721 catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc gggttttccg
     6781 ctcctgaagg caagttctca acgaaaaagc tagcatgttt gatcttgccg atatcatgat
     6841 aatagcaagc tactcgagtt agcaaagaac gtccgccgat ttctgctaca gcgttagcac
     6901 ttagattagc taccatcata ctatgatgat aagtacctgg cgcttcttcc agcaactgtt
     6961 tcaataaagg atgatttgga ttgctgagtt catttaatac aattacactg tcatcattga
     7021 ccatcaattc aatatatggg tgtagaccga tcgtcagcaa gaatgagaag agtgcaccag
     7081 tgaacccgca gacaagcatt aaccaagtcg ttgcatcacc aaagctcatt ccttgataga
     7141 cgatcaaaat gacatccatc atcacaggaa aaataatgac ccacatggca gcagataatc
     7201 cttgttctga gatccgtttg cgtctgacaa tcgttgccat gagcccagag aaaacataag
     7261 acatcaagat aatcgttaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcag
     7321 atactgcttg gaaaatacca gccatgatcc ctgctcttcg gttcaagaaa aagcttaaaa
     7381 caagtggtgc aaaagcagcg ggataaaaca gcgggatata agtagcttgt tccgtttgga
     7441 ataattggaa aaatttcatc agcactatgc tgactgacat agctgtcaca tagaaaagca
     7501 cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc agtacgccga
     7561 tttgaagcaa aacagctaaa accatggcaa tgagtgggaa aatcgaagtc gtttgattcg
     7621 tcaatcctag taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa
     7681 caatgatttc tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta
     7741 attcttctgt ctttttctca ttcaatacat cattgactac aatgccttga tccactaagt
     7801 agcgaatcat ttgttgctgc tctgttgtga cattcaaata ctgaatctga tcttctgctt
     7861 cttgtttaaa agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga
     7921 gcttcaaact ttcatcccta accgtgtgaa tttccgtttc agtcatttca aatatcgtcg
     7981 aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa
     8041 aatttttctt cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt
     8101 ccttcgcctt atctaccttg tcattataag atttgttgat ggtatcattc gtcttatcga
     8161 tcaattcgaa taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag
     8221 tatattctgg agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt
     8281 tctcgatcgt tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcat
     8341 tggccttttg tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca
     8401 gaaggaaagg caatgctgcc tttccgagtt tttcttgtag tttctgcagt gttttattca
     8461 acatgtgctt atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc
     8521 tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa
     8581 atcgattttt ttgatttgct ttagtgttcg ttctgcatga acaagaccac ttgtcgtccc
     8641 tcgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag
     8701 acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac
     8761 gaatgcatca tccaatgtcc gcccccgcat gtaagctaat ggtgcgattt cgattacgcc
     8821 tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac
     8881 aggccgcaga taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc
     8941 ccctgcctct acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc
     9001 agcaattgct aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa
     9061 tacgatgtca tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg
     9121 tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc
     9181 taatgtacct ttttgcccca ttttcaatgc cgtcacgaca tctggtgtac ttacgtggat
     9241 tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga
     9301 ttgttcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc
     9361 ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc
     9421 tgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt
     9481 cgattcattc tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg
     9541 ttgtaaaaga gtcgtttaac catacgtacc aagaaacgtt aaaaaatagc tgtttccatt
     9601 cgctaaaggt ctcgttttga taaaaagaat ctgagaatgt aagttatatg aaagtggaga
     9661 acaaaatatt ccgtttcatt cagaggtatt ctctaggtat ttttcttgtt tcaaaaaaag
     9721 aaaacaaaat agttaaaata ttcttgcatt tcaaaaaaaa caggtgtatt attacgtcaa
     9781 caaataaaca tacagcaaag cgtcgaggaa aacagtaggc tattcaacga tttattccca
     9841 gtgagtcatg taagtgagaa atgacaataa atcgaataga cgaacatcat ttccgagtca
     9901 atgtcgaaaa cgtagatcat tgtggctgcc tccattatca ggctagcgga taactttatc
     9961 aaaaagcgtc cgtgaatgaa gaagcttttt taagcgaaaa aaggtggtac cgttctagaa
    10021 tctagagccc ttttcccaag tggaaaaggg ctttttttta tattttaagg tggtggtttt
    10081 catggaatct gatattagtg gcattactaa ttgctgcaac gtttatatcg tcaatcaaaa
    10141 tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaaacaacta
    10201 ttgatggtca gttcacttat cttcgggatg tttttcgggt caggtaatct aatctttcct
    10261 gtccacttag gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg atttgccatc
    10321 tcaggcgctc ttttcccatt actagctatc ttagctgtag tagtgacaaa aagtgatggt
    10381 ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt aatccttgtt
    10441 catttgacga tcggaccatt tttcggtaca ccacggacag ctgctactgc ttttgaaatg
    10501 gctgctaaac catttttgcc tgaaaaatat aattcattag gtatgttttt attctctgct
    10561 cttttctttt tacttgctta tttccttact gtccatccca cacggttggt caaatacgtc
    10621 ggaaagtatc taaactcctt gtttcttgca ttattagcaa tcatttttgt cgtttcttta
    10681 ttccacccaa tgggaaatct aaatcaatct gctgcagata cttaccaaac aaacgcttta
    10741 acaaatggtg tcctagaagg ttataacacg gttgatgcag tagccttgct tgccttaagt
    10801 gtcacttttg tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt cacagaatta
    10861 acagcaaaag ctggtagcct gagcgttgcg ctagaagtca tgatctattt tggtcttgtt
    10921 cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt
    10981 tctcagatca ctaattttta tttaggtaac ttcggcgcta gcttcttagg agtgatggtc
    11041 acattaggtg tcttcactac cgctatggga ctcgttgttt cttttgcaca agatttccat
    11101 aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt cgtctctttt
    11161 gtcgttgcaa atgctggttt ggacaatatc atccaatggt ctttaccagt attgatgctt
    11221 ttgtatccat tgtcgcttgc gttgattttg ctttccttaa cagcaaaatt tttccaaaag
    11281 acaccatttg tctatcaagt aacgatgttg tttgctgctg ttcccgccgt attggatatg
    11341 ctggcaaatt cgcctgcttt agtgagtcaa caaagagttg tagcaagcat gctcgaattt
    11401 tatcatcatc acgttccttt tgctgcattg ggacttggct ggatggtccc aacgttactt
    11461 gggtatgctg gcagcttact cttctattac gcctatcgcc tatcagggta taaacaagaa
    11521 gcaaatgaac taccagaaga ataaccagat caaacacaat ttaacataag aaatattaaa
    11581 caggaaaaaa acagctagat gaaagaatcc tattctttcg tctagctatt ttttagatct
    11641 cttttttacg agcacaaaaa aactgcggac aatgattgtc cgcagtttaa atgataaaat
    11701 taagattgta atagttcttt cacaatggca tttacttggt ttccatctgc ttttcctttg
    11761 actttaggca tgatcgcacc catgactttg ccaaattctt tcattgatga agcaccagtt
    11821 tgcgtgattg cttcttgaac aagttgtcga atttcttcgt cggaaagttg ttccggcatg
    11881 tatttttcga ctatcacaat ttcacttttt actttttcag ctaaatcgtc acgtcctgct
    11941 tcttcaaact catgcaaaga atcacgtcgc tgtttcattt cgcgagacaa caccgttaat
    12001 tcttcttctt ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat agatgacttg
    12061 atcattcgga tgacttgtaa agattcctta tctttcgctt tcatcgctgt tttcatgtca
    12121 tcgtttaatc tacttagtag tgacacacta ccaactccaa ttcatcacag aactagaatt
    12181 ttttgcgttt tctagctgct tcagattttt tcttgcgtct tacacttggt ttttcgtaga
    12241 attcacgttt acgagattct tgtaaagtac ccgcttttga cacggaacgt ttgaagcgac
    12301 gaagagcatc gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg ttaattccct
    12361 ccctccgagc ttaaaaagta accgtaattg ttattgaaat atatctcaaa aaacaagact
    12421 gtcctgtaaa tattatatct aatgctgttt tgaccgtcaa ggaaatcttg ctttttttta
    12481 aaattatcaa atgttatttt atttaaatcc ttattgacaa ttttcacatc ttccgaaaat
    12541 ctcgaatcta tgcccttcga tcgtacatcc accgagctgt tcttcgaaat aattcattgg
    12601 acacatatgg atttctttcg ttgttccgca caccgtacag ataaagtgat ggtgatggcc
    12661 gatttcccct tgacagcaat gaaaacgaaa cttcatctct ccatttaaat cggtttcttc
    12721 cagtaaatcg atttctgcaa aatcatggag atttcgataa atcgtatcgt aacttactcc
    12781 tttgaattcc tgattcatat agtcaaaaac ttctttagcg gatacgtagc gattgcgctt
    12841 cactaaataa gttaacaatg cttctctttt tttagtatat ttaaaaccgt tatttttcaa
    12901 tgtttctagt gattgttcga tcaacgtctg ttttttcatg acttattcct ccaaatcaaa
    12961 atgattacga agtacacatt ctagagtatg gtataataaa cataaaaaaa atgcaagtga
    13021 ggttttatcc atgtcttccg attctacaga aatcgttact ttttttgtca tctccgactc
    13081 tgctggagaa actgcgacaa aactagcgca agccactatg gcccaatatc ctagcgttga
    13141 gttcaattta ttccgcagaa catttgtgac agatgagaaa acattgatgc aggcactgca
    13201 agatgcacta gaagaaaaag ctcttgtcct gcacacattg atcaatcaag aattgatcga
    13261 gatgacgcgt tctttttgtg aagaacatca attatttagt tttgatgtga tgactccgcc
    13321 gatatcagaa atcgagcgct taactggtat caagcccatt cggcaacctg gcgcccttca
    13381 tttattgaat gagaactatt tcaaacggat caaagcaatg gaatttgctg taaaatacga
    13441 tgacgggaaa gatcctcgtg ggtttctaga agcagatgtc gttttgctag gcgtatcccg
    13501 aacttccaaa acgcctttga gcttgttttt agctaacaaa aatttaaaag ttgccaatct
    13561 acctttgatc ccacaagcac atattcccaa acagctttgg gagatcgatc ctaaaaaaat
    13621 cgtgggactg acgaataacc cagatatttt aaacaatatc cgtaaagaac ggatgcgctc
    13681 ttacggatta aatcctgata ctgcttattc cgatatcgaa aaaatacgag ctgaactcga
    13741 ttttgcgaat gatctttatg aaaaattggg ctgcgtagta atcaatgtcg cttctttatc
    13801 tattgaagaa acagcttcgc ttattttgaa tgctttagac ctagaagacc atagttatta
    13861 tggaactgaa accaccgaag aaacagacta acaatttttc tttaaaaaaa ctggactgtg
    13921 tcagcgaaga agattcgttg acacagtcca gtttgtctct atttgcacct atcgttattg
    13981 atgttccaca tgtttgagtg tctggttcaa aatatccagt acgtgagaat catcaagact
    14041 atacaaaatc gcttttccct cacgatggga cttgacgaca tggttttctc ttaataaacg
    14101 cagttgatgc gatacagcag attgttccat ttccactgct tggatgattg ctgtcacatt
    14161 ttgttccccg tctttcagat acagcagaat tttgagacga gtggggtcac tcaacatttt
    14221 gaatagttgg ctgactttct ctacttttgt tttttgcgtg tctggcaatc cattcacctc
    14281 caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc tcaagttttt
    14341 ttattcttct gtcatctttt ggataatcgt ttcataagcg gctacttttt cagctgcgtc
    14401 ttctgaagag tttcctttta caccgatata aaatttgatt ttcggctcag ttcctgaagg
    14461 acggatagcg atccagccat tttcttccag cgtatacttc aatacgtcag atggcggtgt
    14521 cgttagtttt tctacgtttc cttcacggtc tgttctcgtc aactctttga agtcttctgt
    14581 ttggatgact tttgctccgg caaattctgt tggtgcttct tcacggaatt tttccatcaa
    14641 agcagagatt ttcgcagaac cttcttgtcc gctcattgtc acagaaatcg ttttttctgc
    14701 gtagtagcca taggtttcaa aaatcgtttg caagccatca tacaacgttt tgccttgttt
    14761 tttatagtaa gctgctactt ctgctaataa gagcaatgct tggatcgcgt ctttgtcgcg
    14821 aacgaaaggt ttaactaaat aaccgtaact ttcttcgaat ccgaacataa aagtatggga
    14881 atgatcttct tcaaattgtt gaattttttc agcaatgaat ttgaatcctg tcaatacatt
    14941 gaacatggta gccccatagt gttccgcaat tgctgtaggt aattcgctag aaacgatcga
    15001 tttcaatacc gctgcgtttt caggtaaagt gcctcgcgat tggtgtgctt ccaaaatata
    15061 ttgagcaaac agtgctccga tttggtttcc ggtcaatact tcataggagc catttggcaa
    15121 acgaacggct gcacctaaac ggtctgcatc aggatctgtt gcaatcagca aatcagcgcc
    15181 ttctttctcg ccaagtcgta tcgcatactc aaaagcagag tgttcttctg gatttggtga
    15241 tttgactgta gtgaaatctg ggtctgcgac agcttgttct ggaactagta cgaattgttc
    15301 gaatccagct tgtttcaacg cacgttcgcc caacattttt cctgttccgt gaagtggtgt
    15361 gtacacaagt ttcaaatctt tccccattgt ttccaccaat tcatgatcaa tcgttactgc
    15421 tttgatttct tctagataag cggcatccac ttcttctccg atgatcgtga tcaatccgct
    15481 atgttcagct tcttcgtcag ataatacttc aacttttaac ggattgctga cttcacggac
    15541 atatttcgtc aatgcatctg cgtctgcagg aggcatttgt cccccatctt ctccgtagac
    15601 tttatatccg ttgtaagctg ctggattgtg ggatgcggtt accatgattc ccgcaaacgt
    15661 cttcaaatga cggactgcaa aagaaagctc tggtgttgga cgtagacttt cgaatacata
    15721 agatgggata ttgtgatgtg ctaatgtttt ggctgcttcc atggcaaatt ctggtgattg
    15781 atggcgtgag tcataagcga tagcgacacc tcggcgtttt gtttcttcgc cttgtgtatc
    15841 catgaaacgt gcaagtcctt ctgttgcttg tcttaccgta tagatgttca ttcgattgat
    15901 cccaggccct aagattcccc gcatgcctgc agtaccgaat tccaacggtg cataaaaagc
    15961 atcttcaagt ttttcaggat cttcacccag atcatttaat tgttttttta agttttcttc
    16021 taaattttct tcatgtaccc attgttcata cacttgttcc caagacatga aaataacacc
    16081 tctttctgaa atatggttct taataagtat agcattcact tattagatat gaaagtatga
    16141 ggaccaaaaa acgcttaaat caagaaagga ttcttttatc aagcacggtt gcaagtattc
    16201 gcataaatcg gattcagtag atttcacgcg aaaaaaaaga gtaaaagaac aattaaaccg
    16261 ttcttccact ctttttaaac ttatgcagtt gtttcttcta aagattccaa gtatttatac
    16321 acttgttgtc cagcgatccc accatcacca acagcagtag tgatttgtcg caaatctttt
    16381 tcacgaacat ctccgatggc aaaaacgcct ggtatattcg tcttcatttc gttgtctgtt
    16441 ggaatccagc cagcttcgtt cgtcaaccct gcttctctaa atggtgcagt taatggatcc
    16501 agtccaacat agatgaatac tccgtctgct gccaaatcaa actcattgcc ggttttgaca
    16561 ttacgtgctt tcacacctgt cactaccatt tcattcccaa cgatttcgtc aacgactgta
    16621 tcccacataa aggaaatttt ttcattcgca aatgcacggt cttggatgat cttttgtgca
    16681 cgtaattcat ctctacggtg gacgatgact acttcagaag caaattgtgt caaatagatg
    16741 gcttcttcta ctgctgagtc tcctcctccg atcactacta gacgtttgtt acggaaaaac
    16801 gcaccatcac aaactgcaca gtaagaaacg ccacgtccag caaattcttc ctcgccaggt
    16861 acgcctaatt tacggtggat acagcctgtt gcgataatga ctgtttttcc ttcgtaagtt
    16921 ttatcttcac aaataacttc tttgtggctg ccgtgatcct tgatatcttg cacgattcca
    16981 taagcatttt ctgtcccaaa tcttgaaaca ccttcataca tcttataagc tagatcagga
    17041 cccattattg aatcgaaacc aggatagttt tcgatttctg ccgtattgtt catttggccg
    17101 ccaggggcgc cttgttcgat catcaatacg gataagtttg aacgtgaagc atataaagca
    17161 gcagtcatcc cagcaggacc tgcgccaata acgattacat catacatatg tagattctcc
    17221 tttacttaat caagtaagca tactttacag ccaatgcgaa aaaaagtcta ttgttctgct
    17281 tgcgtttctc gaatagcttt attatactcg aaaaatccgc tgaaaaacaa tagactttta
    17341 taaatcattg atcaatcatt cgaaagattt tctccatttg aatcaattac ttttttatac
    17401 cagttgaatg atttcttctt cgaacgggct aatgtgccat ttccttcgtt gtcacgatcg
    17461 acataaatga atccgtacct tttcttcatc tcacctgtac cagctgatac taaatcaata
    17521 catccccaag tcgtatagcc taatagatcg actccatcta attctactgc atctttcatt
    17581 gcctggatat gtgctgccag atagtcgatt cgatagtcat cttccacata accattctca
    17641 tcgggcgtat cgactgcacc taagccgttt tctacaataa atagtggttt ttgataacga
    17701 tcataaatat cattcatcgt tattctcaag cctaatggat caatttgcca accccattca
    17761 cttgcttcca gatatggatt tttgacagaa gcaaagatat ttcctgcagt ctgttccaag
    17821 agttcgttgt cagtagtcga aactcgagaa gaataataag aaaaggagat aaaatcaaca
    17881 gtatgttctt tcagcaatgc ggcatcccca ggttccattt tgatctgaat gccatttcgt
    17941 tccatttctt tcaatgcata agcaggatat tctcctcgtg actggacatc aatgaagaaa
    18001 taattttctc gatctgattt tctagaagcc caaacatctt caggattgca agtgtatgga
    18061 taatatgctc ctgctgccag catacagcct actttgttct ccggatctac ttcatgagca
    18121 atcttcgttg cgatggcact tgcaaccaac tcatgatgtg ccgcttgata tttgatttgt
    18181 tcttcatttt ctccttcttc aaaatacagc cctgcgccca tgaatggtgc atgaaggatc
    18241 atattgatct cattgaatgt caaccaatat ttgacaagtc ctttatagcg attgaagatg
    18301 actcggcaca gattttcata aaaaccaacg agttctctgg aacgccacgc accatattct
    18361 ttgatcaaat gcatcggaca atcaaaatgt gtaatcgtca caagcggctc gatcccgtac
    18421 ttttgacatt ctttaaagac atcctcataa aatttcaaac cagattcatt tggctctttt
    18481 tcatcgccca ttggaaaaat acgactccaa gcaatggaca tccgataagt cttgaacccc
    18541 atttctgcaa ataaagcaat atcttcctta taatgatgat acatatcgat cgcaattttt
    18601 gctggataaa aatgattttc atcaaattca aacattttct tttttccagt aatgacagga
    18661 aaacggtctt ctccgattgg cacaacatct acgttcgcta acccacgtcc gccttcatca
    18721 taaccgccct cgcattgatt ggctgctgta gctccgcccc ataaaaaatc ttttctgaat
    18781 cccatcatac ttcctccatt acatgtgtat tgcttgataa ttctcttgcc agttcatctt
    18841 ggacttcgat tccttctgct tttaaccggc tgattgtttc cttgaattgc ggtaaatact
    18901 gctgatgtgc aataaacaac tcattcatga tccgttttgc tgtctttcct gatcgtataa
    18961 gcggattgat cgtaaacgct tgtaaagctg ttccataatc accagtcaca gctgcttcga
    19021 tcgtcagcaa ttccatattc ttcattactt gcaaccagcc tctttcagca gcaggaaggg
    19081 aaccaaacgc tacatttcgt gcgccttgtg cgccaacata ggcagtgact tctactacac
    19141 aatctgccgg cagatcagga acagctccat cattctttgt agataccacg atctgtgttt
    19201 ctttatttcc atagatcgaa gcaatcgttt cacaggcagc atctgaataa taagcaccac
    19261 ctcgtttttg caattgttca ggtttatgat ccaattctgg atctttgtat aattcaaaca
    19321 gctctgcttc tgtttctttc acttgctgcg ctcttgtacc aatcgtttga tattcttcta
    19381 aagcatgttc cagcatttct tcttcacggt agtaatagcg gtggtagcca caagggatca
    19441 tgttcatttg ttttaattgt tctttgaaaa acgggatatc aaagatattt ttcggcaatc
    19501 cattgtcttc atcgtataaa cgatcaatca attctgccgt gcggtcattt cctttattat
    19561 ctgctacttt gtgccaatgg aagtgattta gaccggcaaa cttgtagatc agctcttctt
    19621 ttttcttgcc aagcattgtt ggttctgtca tcatcgccat tactggcaca ttgcataatc
    19681 ctatcacttt atcccatttg ccatatctga taactgcttc tgtcaccatc ccgcttgggt
    19741 tcgcgaaatt gatcagccaa gcatttggac ataaacgctt catgtcttca acgattgcca
    19801 aaaccactgg aacggtacga aaagctttga atatcccgcc agcaccattt gtttcttgcc
    19861 caatcatccc ataataacta gggatccgct cgtctttgat tcgtgcatct aattgaccga
    19921 cacgaaactg agtcgtaaca aaatccgcat ctttcaatgc ttcttcccga tccagagttg
    19981 catagatttt gacttcatac ggagaagcat cccacatacg ctgagacatc tgactgacga
    20041 tctcaagctt ttcttttcct tcttctacat ctacgagcca gatttctcgt atcggtaatt
    20101 cctcatatct tttgataaat ccttccatca actcgggggt gtaactgctg cctccaccaa
    20161 tcgtagcgat tttgattcct tttgccatgt tctcactcct ttacttttta cacttttatt
    20221 atcttttttg taaacaaaac aaacaataga aattcgtttc tttccagtaa catttacaaa
    20281 agttagtgtt ttctactgga tttttgtaaa tatcttgtca aaaaatgtct ttattaagct
    20341 atataacata aatccgttta caaatcattt tcaaaaaagt tataatttac gtaaaaaaac
    20401 aagaagaagg tatctacatg ttattacgtg aaaaaatcaa agaaatctcc ttttctcctt
    20461 cagaaaatga agtgattcag tttctcttaa gcgattctcc agcacttggc gaaatgacca
    20521 tccaagagat tgctgagcga tgttatgtcc acccgtccac actcatccgt gtagcaaaaa
    20581 aaatgggttt tgatggctgg gtcagttttc gtaatgcttt tttagctgaa acagcctatc
    20641 ttgaagggaa ttttcaagac gtagatgcga atcttccttt ttccaccaca gacggtatta
    20701 tgacgatcgc aaataaaatg gcttctttag aacagatgac gattcaagat acactttctc
    20761 tcattcatca tgaccaactg caaaaagcca aacaactaat gcttcaagca aaaaaaatcg
    20821 tgattttcgc cagtaactcg aatactttga tttctcaaga tttcatgctg aaaatgaagc
    20881 gcatcaaaat ggatgtcacg attgtcacca cttttggtga atcgttctat gaagcgtaca
    20941 attgcacgag tgatatgtgc gggattctga tttcttatac gggtgaaaac aatgtgatcc
    21001 ttcgtaccgc agaaatattg aaacaaaatc atgttccaat tttagctttg accagtatcg
    21061 gtgattcaac gctatcttca atgagtacag ccgctttgcg acttacgaca cgcgaacgac
    21121 tgtattcgaa aattggtaat tttacgatca atacatcgat ttgttatctt ctagacgtgt
    21181 tatatagttg tgtttttgca gaaaattatc aacagaattt agtccatcta atttctgtag
    21241 gacaaaaatt tgataaacgc cctatcagct cagccatcat gaaagagcct ttcaattttg
    21301 aaaataaaga agtggactaa aaagaataat taagcctagt ccattctttc acctaaccgt
    21361 tcaggtcagt gcggtgataa gtgcatcgtc tggcccgacc gtctcccctt gattttccaa
    21421 aatatccaaa taatccgtgt aattggtgac gatcacaggt gtttctaaac tgtatccttc
    21481 ctcttcgatt ttctttttgt cgaagcggag caacagatcg ccttgtttca ctctttctcc
    21541 ttgctgcacc aagctttcaa aatatttccc ttccaactga acagtatcca atccgacatg
    21601 aatcagcaat tccagacctg cttcagaaac cattccgatt gcatgtttcg tcgggaacag
    21661 ggtcatgact gttccgtcaa aaggggcacg tacttctcct ttttcagggt agatcaagat
    21721 tcctcgacct agtgttcctt gtgcaaaagc tgcatccttt gctgcattca gtggagcgat
    21781 ttgtccttgt atcggtgttt ttacaatttc tttacctgat cttttctgtc tatcatctgt
    21841 ttcacctgct tcctccttcc aaaagaaaaa agtcagcgca aaggatacca ccatcgcaat
    21901 ggcgattcca atcaatacat taatcacatc cgttaaatca ccatctggtc taatatagtt
    21961 gggaattgta aagatgccta atccacctgg attccaagaa gttaaattca ttaatccact
    22021 atacgcgccc cctaacgcac caccgacaca ggtaaaaata aatatttttt tacgtggcaa
    22081 tgtaaaccca taaatagctg gctctgtaat ccccacaata ccagaaacaa tggctggtat
    22141 tgcgattgct ttgtcttttg ggtttctaag cttaaagtag agtgcagtaa gtgctgcagt
    22201 ttgtccaaaa gacactactg caacaggtgt catgatattt tgccaaccat tcacagaaaa
    22261 ttgcatcaat cccattggta cgattgccca atggagacca aacatcacta gaatttgcca
    22321 gaagaagcct acgattgctc cataaataac tggcgaaagt gctaaaagtg tttcaaatcc
    22381 agccattaaa atatcagttg cgaatgtgaa tgctggtcca acgagtaaaa aacctaaggg
    22441 cattgtaata agaacggtga agaatggaac gaagaaattc gcgatcatct caggtactaa
    22501 ctttttaatt ggtttctgaa tttggctagc taacaacaca ataaagataa tgggaatgac
    22561 agaagaagcg tagttacgcg caatccaggg aatgccgaat actgtttgat aaataggtgc
    22621 ttcaaaaata gtgccagaaa atatggtagt cagtggtgct tctgctgcac cactcaaagc
    22681 ttccagcgac aagctaggat tcattaatgc tgccccaagc aacatcccta cgaattcatt
    22741 tactttaaat ttacgggcag ctgttaaacc gataataaca ggcataaatt ggaatacaga
    22801 atcaccgata ttatcaaaca taacaaatgt gccagaaccg cggtcaaatg cgcctaagaa
    22861 gacaagtaat gctgtcagtc cacgaatcat cccaccagca gctagaatag caaggaatgg
    22921 ctggaaacag cccgatattc catcaataag catatcaaac ggtctcattt tttctgttga
    22981 aacagcctca tcaaaagtaa ggtttcctgc ttttataacc tcttcaaaca cgactggcac
    23041 atgattcccg atgacaactt ggtactgtcc tcccgctcgc attaccgtca caacaccgtc
    23101 catatttttc aagatctcat catttgcttt ttcttcgttt tttaatttga aacgaagcct
    23161 tgtgatacaa tgtgtcaaac cattgatatt ttccttcccg ccgacatttt ctacaatttt
    23221 gctcgctaat tcttgatatt ttcccatttc tattccctcc aatttattag tggaaggttt
    23281 agccaactga atgtcactac ccttatttca tgcgttcttg ttaatttatc ctcctttttt
    23341 tatttattta caacgattct catgcactaa acgcgcaata tggatcgtta aatacatttt
    23401 ttcgtccgtg gacatctggt agttgaatat ctcggctaaa aagtctgcta ttttctcaac
    23461 gcaaccaaac gtatccttat attttttttg aataacctgg tacaattcct cagaaacttc
    23521 tcccacttct gatttttctc catctaggac gcgtgtcaga aaaaaacgta ggtgtgtgac
    23581 gaatcgaaaa aaatggactg aatcttcatc aaatgtaatc ttatagtaat agcggacgat
    23641 atttagaata tcctgtattg ttgtagtaaa caggtaggta ttgtcttgtt ggctttgaat
    23701 gccttgtgca ttgactaaat gcaatgcgat aaatccggct tcatcttctg ataacttgat
    23761 gtggtattta tccgagattt tcttcactgt ttttttcccg atggcatact ctttaggaaa
    23821 tagttgtttg atatcataca gcaatacatt tggtacaaat attcccattt tgtgacgttc
    23881 gatagccgta tacaaatgat ccgtcaatgc aatatacacc gaatcattca actggacttg
    23941 tagagcctct tttgtatatt caatgatttc acaagaaatt tctaagtact caagaggaat
    24001 cgcttgtgcc aattcttgga actgttgtga aagtccgtca tttgtcaact gaaatttttt
    24061 gtcgatgcat gagtcaaaaa tcgggtcacc cactttcttt ttgaatgcca aaccttttcc
    24121 catcacgatc atttcctgat ttctttcatc cctggttatt acgacgttgt tattcagtac
    24181 tttctcaatc agcatgtttt actcctttcc atgatatata gggctgtatc caaaacaaaa
    24241 agaaaaaccc aaaaagccgt ccatggaacc atgggagact catcaggttt agcttgcatc
    24301 aggcaatcac tatccaagtt gtatcgtttt ctttatatta tcgaaagcaa gcacttttgt
    24361 caacgctttc ttagcggcta ttgggcactt ctgactagct gtcgtgattt tttttaaatc
    24421 tggtaattac gacactaacg ctcacagatg aagaatctgg gaagctctgt tttattttca
    24481 ctctagcgct taggtcaaaa tgctactatg ctactgtccc aacttctaat atacggtgtt
    24541 ccagtaccag cttatcggtg ataagccaaa tttatgcaaa aatctggaaa gcctttttta
    24601 caattttttt cttatcacct aaagctgaac ggagccctgt cacaaccact ttttacacac
    24661 cactgctttg cagtttgtac atatctgcat acaaaccatt ttgcgctaga agtgtttcat
    24721 gattcccacg ttctactata tgacctttgt ctaaaactaa aatcaattct gcatcccgaa
    24781 tcgtggacag ccgatgggca atcgcaatcg ttgtccgtcc ttgtcgcatt tttgtcaatc
    24841 cttcttggat caatccttct gtttcagtat cgatatttgc tgtcgcttca tccaatacta
    24901 gtattttagg atctgtcacg attgttcgcg caaaagtgat cagctgtcgt tgtccgctag
    24961 aatagctggc accgtgttcg atcacttttg agtgatatcc ttttggtaat tcatcgatga
    25021 acttgtttgc ttgaacaaac tcagctgcat ggcgaatctg ttcgtctgtg atagttggat
    25081 tcatcatccg gatatttcct gcgatatctc cataaaacaa gaaggcatct tgcaagacca
    25141 atcccatttt cttacgtaac tcttccattg gatattctcg aatgtcatga tcatcaatca
    25201 agatctggcc ttcataaaat tcgtaaaacc gcatcaatac gttgatgatc gagctttttc
    25261 cgcttccggt gtgccccact aatgcaacgg tctctcccgg attagcgaca aaagagatat
    25321 tgttcaatac attgtgctct ccatcataag aaaagcttac attgcgaaac tcgatcttac
    25381 ctgaagtaat cgcttgatct gctccaggat tttgttgcgg tgcatattct tctgtatcca
    25441 tgattttcaa aatacgacta ccggcaacca ttccatctgt aaaggtgctc aaaaagtcca
    25501 tcatttgaga cattggatta aaaaatccct ggacataggt cgtaaatgca tagatcagtc
    25561 caacttctac gggagaatgc agcgcatcga tcccaaacaa agtcaacgcc aacgcaatcg
    25621 ccaatgcata tagaaaattg atgatcggac ttaacagaat cgaatttgtt ttgatcatcg
    25681 catagcgcgt gtctaaatac tcgttgttca cttgttcaaa ttctttagct aaacgagctt
    25741 cttggcgaaa ctgctggata atacgcatgc cggaaatcga ttcattcaat ttcgtgttca
    25801 gctgacttaa tttttcccgc atattccggt aaatacgaga actgaatttc tggtaatacc
    25861 agatgacgat tcctaaaatc ggcaagaaca ccaaagtcca caaggcaatt tctccattga
    25921 tttgaaacat ggcaatgaag gaagaaacga ctgcaaagat cccagtcaat accatcaaaa
    25981 agacatacca aaattcaaat agcgtttctg tgtcatttgt cactcgagaa acagtcgatc
    26041 ctgccggtgt ctgatcaaaa taacgcatac ctagtgtatg cagcttttca aataatctta
    26101 cccgaacata ctgataggtc ttcaatgaag ccatggaata caaataccat tggaaaaacc
    26161 aaatgatact tttgactatg actccaaaca agtacatacc agcaaaaaac aaaataactt
    26221 gtgtcgtagc cgactgttta gccagatgat catccataaa agtttggatg atccttggca
    26281 ataggacatt gatcacagac agggcaaacg caaataagat agcaacgatg aacgttcttc
    26341 taaaaggttt ggcgaacttc atcagtcgtt tgatgatctt cttctgttct ttaagtggaa
    26401 tcgattttgt ccattccgat tgataatttt catccatcag gcctcgcctc cttctatctt
    26461 cgcttctaat ttctgtttct cccacattcg ttcataccag ccgcctaatg caagcagctc
    26521 atgatgggtt cctcgttcaa tgattttccc ttgatcgaca acaatgattt cttttgcatg
    26581 catcacgctg cttaaacggt gagctgcaat gatggttgtt ttctcttttc ttatttcttt
    26641 taaattatgc aagatagctt cttctgtttt ggcatctact gcagaaagtg catcatctaa
    26701 gatcagcaat tcaggatttt tgatcaatgc tcgagcaatc gaaatccttt gtttctgtcc
    26761 gccagataaa gagactccac gttctccaac taaggtatca taaccttttg gtaattcctt
    26821 gatttcattg ttgataaatg ctaaagaagc cgcatgctct acttcttctt ctgaaagttt
    26881 tggattagaa aaacggatat tatctcgaat ggtcatcgaa aacaagaagt gatcttgagg
    26941 cacatagccg attgatccta ataaagcatt caatgaatat tctttgatcg aacatccgcc
    27001 aaattgaatt tttccttgat aatcatcgta ttcacgcatc agtaatttga tgattgtcgt
    27061 ttttcccgca ccagttttcc caacaatacc taatgtttct ccttcttgga tcgcaaattt
    27121 gatttgttct aatgctggtg attcttcttt cggatagtta aaagaggaaa tagctacatt
    27181 cagctcgcct tttgccggag tctgcactgc acccagttgt tccacgatat gcgtcttttc
    27241 atgcaaaagt tcattgacgc gatcataaga agcatttcct ctttccaata cgttgaacaa
    27301 tcgaccaatc gcaaacatcg gccaaacaag cataccgata tagctaataa atgagaccat
    27361 ctgcccgatt gagatcgtgc cttccaagac atactttcca cctaaaataa ttgttgcaac
    27421 ataagacaga ccgataatca aggtaatgaa tggatcaaat aaggagtcta aaaaattgac
    27481 tcgtttgttt ttaataattg catcttcaat cttttcttca aaatccgcga gatcttcttt
    27541 ttcttgtcca aatgttttaa tgactttgat cccagtaata ctttcttgtg tcttatcatt
    27601 gatactagaa aaagcttctt gcgaatcacg aaaagcatcg tggagttttg accccagcac
    27661 tcgtgaagta acggctagta aaggcagagg aatcagcgca atcagtgtta agcgccaatc
    27721 aataaacaaa atcatcgcaa tgatcgttgt tccgcccgtg atcactgaat ctgcaaaggt
    27781 caagatacca gctcctgcta cattttggat tgcattcaaa tcattcgttg catgagccat
    27841 caaatctcca gtccgatatt tttggtaaaa gagattgtcc atttctgtaa aatgtttgaa
    27901 tagctgctgc cgaagttctt tctccaaccg agctgcactt ccccaaatat tcgtccgcca
    27961 gatataacga aaaagatatt gtaagagagc tgcaaggatc aataataaga tccaccagga
    28021 aatgatcttt attgataaat ttttatcggc gatctcatca atgatgatcc caatcacttt
    28081 tggaggagct aactgtacca aggccactgc gaatagggag aaaatcccaa ttaaataatg
    28141 tttcttttcc tgtcgaaaaa accaacctaa ttttctaaaa atcgacattt tactcctcct
    28201 ttgtctttat caagagcata ggattgtctg tgcttgtctt ttttgctgaa tagtctgaat
    28261 agtctaacaa attacgaaat aaaaaacgga ctgaaaaaat cagcccgcaa aaagtctttg
    28321 ggtcagaacc tattttttcc cttggttctt catttgctgc atcatctggc gaattttctt
    28381 ctcagatggt ttttgcccca ttgacatcat cattgatcgc aacatgtcct cattaacggg
    28441 aggatttttt tctaaatagt ctttcatata tttacgcgct aggaaaaagc cacctactgc
    28501 gccgattagc aaagcaataa tagcaataag tacgacaata cctgttgata ccatataaat
    28561 ctctcctttc tatttccgaa ttgctctcaa tgatttattt tactaaagtt tattgaaaaa
    28621 agaaaggcgt attctttagt ttcatgcaaa aaatagtggt ttttttagag aagaacaaaa
    28681 tttctttcat taaccagcaa gacacgccac gaaaaaaaat taggcatacc aaatcttttt
    28741 ctttaatttt ttctttctga ggtgtagaat gtaatcgtag ataaatagga aaggtggttt
    28801 tttcttgaat aatcaatcca acaaacaatt tcataagcat cgacttattg aacatacaaa
    28861 tggtttatct ttagctgaag taaatggaac agtggatgta cccaagggaa aaagtttttt
    28921 taaaacactt cttgcttact ctggaccagg tgcattagta gcagttggtt atatggatcc
    28981 aggtaactgg tccacttcga tcactggtgg acaaaatttc caatatttat tgatgtcagt
    29041 tattttgatg tctagtctga ttgctatgct tttacaatat atggcagcaa aattaggaat
    29101 cgtgacccaa atggatttgg cccaagcaat ccgagcacgg acaagtaaga cattgggtgt
    29161 cattttatgg atcttgactg aattagccat tatggcaaca gacatcgcag aagtcattgg
    29221 tgcagcaatc gccctctacc tgctttttga tattccattg gtaattgccg tatttatcac
    29281 tgtgttagat gttttcgtcc ttttattgct gacaaaagtc gggtttcgta aaatcgaagc
    29341 tttggttgtc tgcttgattt tcgtcatttt attcgttttc gtctatcaag ttggcttgtc
    29401 taaccctgat tggcacagtt tgtttgccgg atttatccct aacagtcaca cattctcaga
    29461 ctcaccggtc atcggcggcg aaactccgct gactggagct ttagggatta ttggtgcaac
    29521 agtcatgccg cataacttat atcttcattc agcagtttct caaactcgga agatcgatca
    29581 ccaagacgaa gaagatgtag cacaagcggt acgcttttct acttgggatt ctaatatcca
    29641 gttgtcaatg gcttttctag tcaatgcact gctattgatc atgggtgtgg ctgtattcaa
    29701 aaccggttct gtccaagatc cttccttttt tggtctgtat gaagctttat ctgatccatc
    29761 agctatgagc aatggtatct tgattgctgt agcaaaatct ggtatcttgt ctactttatt
    29821 tgctgtagca cttcttgctt ctggccagaa ctctactatt actggtacgt tgaccggtca
    29881 agtcatcatg gaaggcttca tccatatgcg tatcccaatt tggctaagaa gactagtcac
    29941 acgattgctt tctgtgattc ctgtattgat ttgtgttttg tttacaagta caaaaggaac
    30001 gatcgaagaa cataccgctt tgaataactt gatgaatgaa tcccaagttt ttctggcttt
    30061 tgcattgcca ttctcgatga ttcctttact gatgatgacc aatagccaag cggagatggg
    30121 gagccgattc aaaaattcat ttgttgtaaa actacttggc tggttctctg tgattagtct
    30181 gacttacttg aatttacgag gattaccagg tcaaatcgaa agtttcttcg gcgagcaagc
    30241 cacctcagct caactagcat tggctgatca aatcgcttat gtaatcattg ttcttgtcct
    30301 tctcctgttg gcatggacga ttggtgattt atataaagga aacaaaaaat atgaacagcg
    30361 tttgcaggaa acgatagaag aaatcgagta gaaactgatt cttttacaca aaggaggttg
    30421 tgtcagaagt gggcagcttc aagaaataag aaggaattta agaaaattgc ttttcaaatt
    30481 tttgtgaaat ttcggcttat ttccgaagaa gttacttctg gaacaccgtg tatcagaggt
    30541 tgtgaaaaag ctgccttgca tcaagtaata agaacagcca agtaaaaaca gctttttatg
    30601 tttttaactt ggttggactt attaccgcag atgcaagctt ttgagcaccg tttattcgga
    30661 aaaaggaact gcgacaagac ttttgtcaca gttccctttc ctacgattag tcaagtaaaa
    30721 ggtgtaagaa aaatagctca taaacgaaag cgacaaaatc aa
//
LOCUS       DASJRQ010000023        29430 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJRQ010000023 DASJRQ010000000
VERSION     DASJRQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.023"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            169..1314
                     /gene="tgt"
                     /locus_tag="U4A80_000970"
     CDS             169..1314
                     /gene="tgt"
                     /locus_tag="U4A80_000970"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1974603.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1411..1791
                     /gene="yajC"
                     /locus_tag="U4A80_000971"
     CDS             1411..1791
                     /gene="yajC"
                     /locus_tag="U4A80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1974604.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            2096..4693
                     /gene="adhE"
                     /locus_tag="U4A80_000972"
                     /gene_synonym="adhC"
     CDS             2096..4693
                     /gene="adhE"
                     /locus_tag="U4A80_000972"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1974605.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            4922..6028
                     /locus_tag="U4A80_000973"
     CDS             4922..6028
                     /locus_tag="U4A80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1974606.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            6045..7073
                     /locus_tag="U4A80_000974"
     CDS             6045..7073
                     /locus_tag="U4A80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN1974607.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            7079..7558
                     /locus_tag="U4A80_000975"
     CDS             7079..7558
                     /locus_tag="U4A80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1974608.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            7574..8869
                     /locus_tag="U4A80_000976"
     CDS             7574..8869
                     /locus_tag="U4A80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN1974609.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            8880..9704
                     /locus_tag="U4A80_000977"
     CDS             8880..9704
                     /locus_tag="U4A80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1974610.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            9869..10768
                     /locus_tag="U4A80_000978"
     CDS             9869..10768
                     /locus_tag="U4A80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974611.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(10894..11763)
                     /locus_tag="U4A80_000979"
     CDS             complement(10894..11763)
                     /locus_tag="U4A80_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN1974612.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            11916..13376
                     /gene="rhaB"
                     /locus_tag="U4A80_000980"
     CDS             11916..13376
                     /gene="rhaB"
                     /locus_tag="U4A80_000980"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN1974613.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            13373..14629
                     /gene="rhaA"
                     /locus_tag="U4A80_000981"
     CDS             13373..14629
                     /gene="rhaA"
                     /locus_tag="U4A80_000981"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN1974614.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            14643..15488
                     /gene="rhaD"
                     /locus_tag="U4A80_000982"
     CDS             14643..15488
                     /gene="rhaD"
                     /locus_tag="U4A80_000982"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN1974615.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            15490..15801
                     /gene="rhaM"
                     /locus_tag="U4A80_000983"
     CDS             15490..15801
                     /gene="rhaM"
                     /locus_tag="U4A80_000983"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN1974616.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            15883..17034
                     /gene="fucO"
                     /locus_tag="U4A80_000984"
     CDS             15883..17034
                     /gene="fucO"
                     /locus_tag="U4A80_000984"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN1974617.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            17161..17919
                     /locus_tag="U4A80_000985"
     CDS             17161..17919
                     /locus_tag="U4A80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN1974618.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            18019..18663
                     /locus_tag="U4A80_000986"
     CDS             18019..18663
                     /locus_tag="U4A80_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1974619.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            18670..19677
                     /locus_tag="U4A80_000987"
     CDS             18670..19677
                     /locus_tag="U4A80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1974620.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            19693..20535
                     /gene="kduI"
                     /locus_tag="U4A80_000988"
     CDS             19693..20535
                     /gene="kduI"
                     /locus_tag="U4A80_000988"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN1974621.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            20555..21358
                     /locus_tag="U4A80_000989"
     CDS             20555..21358
                     /locus_tag="U4A80_000989"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1974622.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(21607..22671)
                     /locus_tag="U4A80_000990"
     CDS             complement(21607..22671)
                     /locus_tag="U4A80_000990"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1974623.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(22668..23753)
                     /locus_tag="U4A80_000991"
     CDS             complement(22668..23753)
                     /locus_tag="U4A80_000991"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1974624.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(23766..24743)
                     /gene="mvaD"
                     /locus_tag="U4A80_000992"
     CDS             complement(23766..24743)
                     /gene="mvaD"
                     /locus_tag="U4A80_000992"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1974625.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(24736..25680)
                     /gene="mvk"
                     /locus_tag="U4A80_000993"
     CDS             complement(24736..25680)
                     /gene="mvk"
                     /locus_tag="U4A80_000993"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1974626.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            25996..26655
                     /locus_tag="U4A80_000994"
     CDS             25996..26655
                     /locus_tag="U4A80_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1974627.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            26740..27645
                     /locus_tag="U4A80_000995"
     CDS             26740..27645
                     /locus_tag="U4A80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1974628.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(27647..28279)
                     /locus_tag="U4A80_000996"
     CDS             complement(27647..28279)
                     /locus_tag="U4A80_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1974629.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    28430..28555
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            28599..29123
                     /gene="infC"
                     /locus_tag="U4A80_000997"
     CDS             28599..29123
                     /gene="infC"
                     /locus_tag="U4A80_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1974630.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            29195..29395
                     /gene="rpmI"
                     /locus_tag="U4A80_000998"
     CDS             29195..29395
                     /gene="rpmI"
                     /locus_tag="U4A80_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1974631.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
BASE COUNT         9395 a         5278 c         6459 g         8298 t
ORIGIN      
        1 aatatttctg tcataatgtt tttctttttt ttgttattca taaaaaaagg ataaaaaaaa
       61 gaatattaaa taaagtagaa ggaagccgag ggaacttgca aaaagcactt gaaacgatta
      121 tgatggtggg agttgtcaag atattttata tagaaagtag ggaaatgaat gacgcaacct
      181 gccattcgct atcgtttaat caagaaagaa aaacatactg gcgcgcgttt aggggaattg
      241 atcacgccac acggaacttt ccccacacca atgttcatgc cagtaggaac attagctaca
      301 gtaaaaacaa tgtccccaga agagttgaaa gaaatgggtg ctggcgttat tttaagtaat
      361 acatatcatt tatggctacg ccctggtgaa gacttagtag aagaagctgg cggactgcat
      421 aaatttatga actgggatca accgatttta acagattcag gcggctttca agttttttca
      481 ttaagtgata tgcggaatat cgaagaagaa ggcgtgcatt tcaaaaatca cttgaacggt
      541 tcgcgtatgt ttttatcacc agaaaaagca atcaacatcc aaaataaatt gggctctgat
      601 atcatgatga gttttgacga atgtccacca ttcgatgaaa gttacgacta cgtgaaaaaa
      661 tcagtagaac gtacgtctcg atgggcagaa cgaggattga aagctcatgc taatccagac
      721 cgtcaaggat tatttggaat catccaagga gcagggtttg aagatcttcg tcgtcaaagc
      781 gcgaaagatc tagtcgggat ggatttccct ggttactcaa tcggcgggtt atctgtcggg
      841 gaacctaaat cagaaatgaa tcgtgtatta gaattcacta caccgttgat ccccgaaaac
      901 aaaccacgtt acttgatggg tgtcggtgct gctgattcat tgatcgatgg tgttatccgt
      961 ggagtagaca tgtttgactg tgtcttgcct actcgaatcg ctcgaaatgg cacatgtatg
     1021 acttctcaag gccgtctagt agtgaaaaat gcgaaatatg cccatgattt ccgtccaatt
     1081 gatgagaaat gtgattgtta tacatgtaaa aactatacac gtgcctatat ccgccatttg
     1141 atcaaatgtg atgaaacatt tgggattcgt ttgacttctt accacaattt atacttcttg
     1201 ttgaatctaa tgaaacaagt acgtcaagca atcatggatg ataatttgct tgaatttaga
     1261 gaagcctttt ttgaagaata cgggttcaat aaagaaaacg caaaaagctt ctaaaggaaa
     1321 atcttttaaa aattaagtaa aaaactagtg ttatcagcat ttttctgcta aagtgttttt
     1381 tgtagataca gaattagtga ggtgaaaaaa atgggaacat taccaatgct tgtgatgttt
     1441 atcgcgctat tagcaatgtg gttttttatg tcacgttcgc aaaagaaaca acagcaagaa
     1501 cgtcaaaact tgttagatag catgaaacca ggtgattcag tcgtaacgat cggcggatta
     1561 catggagttg tttcagaaat cgacaatgac aaacggacag ttgttttaga ctgcgaaggg
     1621 atcttccttg agtacgatcg tgcagcaatc aaaacggtca aaccagggac tgtcgtaaca
     1681 aatgatgcaa cgactactgt tgaaacaaca gaagaaccag ctgagacagt tgaagtaaaa
     1741 actgaaacaa cagaaacgct ggaagagaca gaagagaagg aagaaaaata agtatcaaag
     1801 cttatttaaa gacgaggtag ggcagaaaga tgtcctacct cattttttat tcaaaaaaga
     1861 aagagataag agcatgggat atattgctca atattgaata atcgggtgtg tcatataaat
     1921 aataataaat aactttatat atttttctat acaaacacct gtaaataaag caattttata
     1981 tttaaaaata actgtgaaaa aaatcacaaa aaagtgttta catagaaaaa taaatattgt
     2041 atgatataca tgtgaattat ttaacaaata accatgaccc attaggagga taatgatggc
     2101 taaagtaata gaaaaagaaa aaacgaagac aattgacgta caagcaatga ttgatgattt
     2161 ggcagaaaaa gcaaatgcag cattgaaaga aatggaaagc tttgatcaag aaaaagtaga
     2221 ccatatcgtg catgaaatgg caatggcagc tttggatcaa catatgccat tagcgaagat
     2281 ggctgtagag gaaacaggcc gcggtgttta cgaagataaa gcaatcaaaa atatgtatgc
     2341 gtccgaatat atttggcata atatcaaaca tgataaaaca gttggtgtga tcaatgaaga
     2401 tgtccaaaaa gggttgattg aaattgctga accagtcggt gtggtttgcg gagtaactcc
     2461 aacaacgaat ccgacttcca caacgatttt caaatcaatg atcgcgttga aaacaagaaa
     2521 tccaatcgtt tttgctttcc atccaagtgc ccaaaaatct tctgcagaag cagcacgagt
     2581 ggtacgtgat gctgctatcg ctgccggagc tcctgaaaat tgtatccaat ggatcgaaca
     2641 tccttcgatc gaagcgactt ctatgctaat gaaccatcca ggtatcgcga tcgtcttagc
     2701 gacaggtgga gctggaatgg tcaagtctgc ttactctaca ggaaaaccag ctttaggtgt
     2761 aggtccaggt aacgtaccag cttatattga aaaaacagca aaaattaaac gtgcagtgaa
     2821 cgatttgatc gtttcgaaaa catttgataa tggaatgatt tgtgcttctg aacaagctgt
     2881 gatcgtggat aaagaaatct atgcagcagt aaaagcagaa ttccaagcac accaagttta
     2941 tatcgtaaaa ccagatgaat tgcaaaaatt agaagatgca gtgatgaacg aaggaaaata
     3001 tgcggtcaat ccatcaatcg ttggacattc tgcgatggaa atcgcaaaat tggcaggaat
     3061 cagtgtacca aaaggaacga aaatgctgat tgctgaattg gaaggcgtag gcccagatta
     3121 tccgctatca agagaaaaac tttctccagt tcttgccatg atcaaagcta acaacacgga
     3181 ccatgcgttt gatttgtgtg aaggtatgtt gaatcttgga ggattaggac atacagcagt
     3241 tatccattca gaaaacgaag agttgcatgt caaattcggt cttcgaatga aagcttgtcg
     3301 tattttagtg aatacaccat ctgcagaagg cggcatcggt gatatctata acgaaatgat
     3361 tccgtcattg actttgggat gcggttctta tggtaagaac tctgtttctc gtaacgtctc
     3421 tgctgtaaac ttaatcaatg tcaaaactgt agcgaaacgg agaaataaca tgcaatggtt
     3481 caaattaccg ccaaaaatct tctttgaaaa gaactctcta ttgtacttag agaaaatgga
     3541 aaacgttgaa cgtgtcatga tcgtttgtga ccctggtatg gtccaattcg gttactgcga
     3601 tacagttcgt gaagtattgg cacgacgtaa aaatgatgtg aagatcgaag tcttctcaga
     3661 tgttgaaccg aacccatcga caaataccgt atacgcagga acgaaactga tggcagactt
     3721 caagccagat acagtaatcg ctcttggagg cggttctgca atggacgcag ctaagggaat
     3781 gtggatgttc tatgaacacc cagacacatc tttctttggt gcaaaacaaa aattcttgga
     3841 tatccgtaaa cggacatata agatcgacaa accagaaaaa acacaattcg tatgtatccc
     3901 gacaacatca gggactggtt ctgaagtaac gccatttgcg gtcatcactg acagtgaaac
     3961 acatgtgaaa tatccgttag cagattatgc attgacgcca gatgtcgcaa tcgtcgatcc
     4021 tcaattcgtc atgtcagttc cagcatctgt cacagcagat accgggatgg atgtgttaac
     4081 acatgcaatc gaatcctatg tttcagtcat ggcttcagat tatacacgtg gattgagcct
     4141 acaagcaatc aagttagtct ttgactattt agaaaaatca gtaaaaacac ctgatatgga
     4201 atcacgtgaa aaaatgcaca acgcttctac aatggctggt atggcgtttg ctaacgcatt
     4261 cctaggtatt tgtcactcag tcgcacataa aatcggcggg gaatacggga tccctcatgg
     4321 acggacaaat gcgattcttt taccgcatat tatccgctat aatgcaaaag atccacaaaa
     4381 acatgcgatg ttccctaaat atgactattt ccgtgcagat acagattacg cagatatcgc
     4441 aaaattctta ggtctgaaag gcgaaacaac agaggaatta gtcgaagctt tagcaacggc
     4501 tgtttatgaa ttaggaaaat ctgtcggcat caacatgagt ctaaaagctc aaggagtcac
     4561 acaagaaaca ctagatacaa ccgtagaccg tatggctgaa ttagcttacg aagaccaatg
     4621 tacgacagct aatccgaaag aaccattgat ctctgaattg aaacagatca tcattgatgc
     4681 ttacaacgga taagcaaaag aaagcgtaga aaaaagataa gaaaatagat gaaaaagaac
     4741 ttggaaaacg ttgtttctcc aagttctttt tttaatttcc aagtaactga aagttacttt
     4801 tgttatttaa catttaggtc aattttagac agtttttgtt caaaaagcga tatttatagt
     4861 ggacggtatt actgctatat taaggatata aaatgaaagc gtttaaaaag gtggcgtaaa
     4921 gatgattatt aaagaagaga tttttgtacc taaagaatta gtgaaagaaa aaattgacct
     4981 gctcgttgaa aacctgacaa cgatcaaaga cgataatggt gaatttttgt tggactttga
     5041 tggactgaaa gtagatgaca aaagctggac agtgtggaat tggccacaag gtgtcggtct
     5101 atatggtatt tataaaaatt accagatgac aaaaaatcct cgcgcttatc aagtggtcaa
     5161 tgaatggttt gaggatcgta tggaagaagg cgctccgccc aaaaacgtca atacaatggc
     5221 accgctattg actatggctt acttgtatga agacacgaaa gattcacgct atttgcctta
     5281 tttagaacaa tgggcagaat gggtcatgaa tgacatgcct cggacaaatg aagacgggtt
     5341 acagcatgcc acatatggac cggaaaataa gaatcagttg tgggacgata cattgatgat
     5401 gacagtgctt cctttggcaa aaatcgggaa actgttgaat cgcccagaat atttagaaga
     5461 agctcgttac cagtttatga tccatatcaa atacttgatg gataaacgaa caggattgtg
     5521 gtatcacggc tggacgtttg aaggaaatca taactatgcc gaagcatttt gggctcgtgg
     5581 aaactgctgg ttgacgattg ctattccaga gatcattgaa atcttagaat tatcgaaaga
     5641 agatgcattg cgcaaattat tgatcgaaac acttgaagca caagtgcgtg cattaaaaac
     5701 ttatcagtct gaaagcggtt tatggcatac gttgttggat gatccttctt cttacgtaga
     5761 atcttctgca actgcaggat ttgcttacgg tattttgaaa gcggtccata aacgttattt
     5821 accacaagaa tataaagaag tcgcttatcg tgctatccaa ggattgcttg aacaaatcga
     5881 tgaaaaagga gaagttcaaa acgtttcaat cgggacagga atgggggaca gtttagattt
     5941 ttatcggaat atcggaatca cagcaatgcc ttatggacag tcgttaacgg tgctttgttt
     6001 gacagaattg ctcgtctctt attgttaaaa gaaaggaaac ccatatgaaa aaaacatcac
     6061 tgctgtttgc agcaagttcg cttagcctag gtctgctagg tctaagcggc tgcggaaata
     6121 attctagtgt ggcaactgtc gcagatgcag atgtaaatga gatagttacc ttgcgctttg
     6181 cttatgcaag caacagccag ccagtaattg atgcaatgaa tgaatttggg cgtttagtaa
     6241 aagaaaaaac agatggttcc gtacagatcc agtatttccc agatggacaa ctgggtggag
     6301 aacgtgaact gatcgaattg acacagtcag gagcagtaga tttcacaaaa gtaagtgcct
     6361 cagcgttgga aagtttctct aaagattatt cgattttttc agttccttat ctattcaatg
     6421 atgaagaaca tttctttaag gtgatggata atgaagaaat aatggagcca atctaccaat
     6481 cgaccaaaaa tttaggattt accggattga cctattatga ttctggtcag cgcagctttt
     6541 atatggtaga cggtccgatc catacgccgg aagatttgga gggaaagaag atccgtgtga
     6601 tgcaaagcga aaccgctatc aaaatggtag agcttttagg cggttcgccc gtacctatgg
     6661 gaagtgatga agtgtacacg tctcttcaat ccaatttgat caacggatca gaaaacaatg
     6721 aatttgtctt gtatacagct ggacatggtg gtgtggctaa atactattcc tatgatgagc
     6781 atacacgtgt gccagatatc atcatcatga atgatgccat caaagaacag ctaactgatg
     6841 agcaggaaaa agccattgaa gaagcagcga aagaatcaac ggtttttgaa attgaagctt
     6901 ttgcaaaagc aattgaagaa gaaaaagctt tagcacaaga agaacatgga gtccaattca
     6961 atgatgtaga taatacaccg tttcgagagg cagttgcccc acttcatgat atattcaaaa
     7021 acgatcctac ctacaaagac ctttatcaaa agatccaaga gcaaggagcg tagctgtgat
     7081 gaaaatgatt ttaaacaagg tattagagat cctgggagct gttttgcttg ttgggatggt
     7141 cgctgttgtt ttatggcaag tattttccag aacggtttta ggaaatccaa atacggtcac
     7201 ggaagagctt gttcgattcg gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt
     7261 gggacaaaaa gcacatttag ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag
     7321 taaaatttta gaagttgttg ttcaaagttt atttctggta tttgctgcga tgatcatggt
     7381 gtatggcgga tggaatgctg tcacattgac aatggggcag atctcgcctt ctttaagtat
     7441 tcctatggga tatgtttatc tgtcagtacc tgtatcgggg gtactcatca ttatctatag
     7501 cctgatcaat ctaatcgata cattaagcaa caagccagca attcaagaaa aaatataaaa
     7561 atggaggaat aaaatggcaa tagaagccgg attagtgtta ttttttgtat ttgttgtgtt
     7621 gcttgtatta gggatgccga ttgctataag tattgcattc tcatcgatga tgactttatt
     7681 gttagttata ccgtttgatg tttctgcttt tagttcagct caaaaaatgg tgggaagtat
     7741 caatagtttt tcgctgatcg ctattccgtt tttcgttctc tcgggcatta tcatgaataa
     7801 tggagggata gctaagaaac tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg
     7861 ggctttggct catacgaatg tgttagggaa tgctttattt ggttccatct ctagctcggc
     7921 cattgccgct tctaccgcga ttggcggagt gttgatccct cagcaagtgg aagaaggata
     7981 tgaccggaag tttgcgacag cggtcaatat cgcgtcagct ccgacaggta tggtcattcc
     8041 tccaagtact gcttttatca tgtattcact tgttgctggt ggtgcttcga tctctgcttt
     8101 gtttatggga ggttatctcg taggcgcttt gtgggcgcta ggaatcatgc tagtagcatt
     8161 catcgttgct aaaaagaata aataccctgt tgtcgaaaaa ggacagatgg ctcatgctgg
     8221 aaaaataata aaagaggcga ttcctagcct tctattgatt gtcatcatta tcggtggtat
     8281 cttgacgggg atatttacag cagtagaagc ttcagcgata gctgtcattt actccctgtt
     8341 gattgctatg ttttactata aaacggtaaa actaaaagat atacctaata tgttgtttca
     8401 atcagtagca atgtcaggga cgatcatgtt cttattggct acttcttcca tgatgtcttt
     8461 cgccatggcg tttaccggta ttcctcaggc aattagttcg ttgattatgg gcgtaacgga
     8521 taataaattt attattctgt tattagtcaa tcttgtgtta ttactggtgg gtatgtttat
     8581 ggatgtggcg cctgctatct tgatttttac accaatattt ttaccaatcg tcacaagtgt
     8641 aggggttgat cctgtacatt acgggttgtt ttcaatcatg aatctgtgtg taggctccat
     8701 cacgccgcca gtaggtacag gactatatgt cggtgcaagt gtaggaggcg taaaagcaga
     8761 ggcaatgctg aaaccgcttc ttccttttta tggggttatc ttagtcgtct tgtttttgat
     8821 tacttacttc ccgcaaatcg tcatgtggct acccaatatg ctgagttagg agaatagtta
     8881 tgttgaacat tacaattaga ggacatgatc tttccgaagt ccaaacgata gaagatcttg
     8941 cagaaaaaac aaaagaacaa gggattcata cactgcagct cgccttaggt ctttcgtttc
     9001 ctgaaacacc ttctggcgca aatgagatca attctgggat gggaaattat gtcaaacgag
     9061 ttttagaaaa gcaagaagtg accgtaggaa ttttaagttg ctatatcaat atgattcatc
     9121 cagatttaac gatacgacaa gagttgctga caaagttcga gcagtatctt cgctatgctt
     9181 cttcctttgg tgcgtcgatg gttgcttcgg aaactggatg tgttttgccg gaaatccaat
     9241 atacggaaga aaatttcaca gatgaagcat ttgctgaagc tgtgtccgtt atccgtcgct
     9301 tggtaaaagc tggagaaaaa taccaaatga tggtggggat agaaccaggt ttgaatcatc
     9361 cggtgtattc tttagcaagg gtagagcagc tgatccaagc agtagattcg gactatctag
     9421 gaatcatttt agatccgacc aatttgatca caagtagtga ttatcaagaa caagtccaat
     9481 tggtagaaga agcttttgaa cgttttggtg aaaagatatg tgcggttcat ttgaaagatt
     9541 ttcgtgtcga acaggaaaaa atcgtgccgg taaatttggg agacggtgtg atcgagtaca
     9601 caaaaatcaa agaaatcatc atgaagaatc gtccgtacct gtatgtggtt cttgaagaaa
     9661 caaaagatga tgggattcgt tatggaagaa gtcttcttga ataatgtaaa gaagacgttg
     9721 gttaactaat atttagaatg gagggggcgg ggctagaaga tgccacgtct cttctttttt
     9781 ataagagtat ataaaagcag ctctttttga ttacaaaaaa cgtggtataa taaaattgtg
     9841 cataagtgaa caaaaggagg tgcggcacat gctccaaaag aaaatcagcg gtgaacaagt
     9901 caatacgatg gtttctgatc agtatgaaat ctatcatgtc aaagatgtcg tcagccaaaa
     9961 taaaacgatc tatcatcatc atgatttcta tgagatccat gctacattaa agggagaagc
    10021 gactttttat ttgaacggac gacaatttac gatcaaagca ggaagtgtgc tgctcatcca
    10081 ttccaatgat ttgcatcgta tcgtccgcca atcttcagat gtattcgagc gtgtctatat
    10141 ctttgtcacc ccttcttttc tagaaagtcg ttccacgcca tggtctaatc tatcggcttg
    10201 ttttcaacca atgggagaga agaggagtcg tgtattgcaa atagaacagg aagtattgaa
    10261 gcaaaaatta tcatttatcg accatcctcc tgataaaaaa atttacggtg aagacataaa
    10321 atatgaacaa gctctggtag aatacttgat ttttctcaat caagttgtac aaaaagaaga
    10381 gagtgcgcaa gaacagaatg ctgtagttcc taatgagcgt gtggaacaaa tgttacaata
    10441 tatctcaaaa aatttatctc atccgctttc tttagaggaa atggaaaagc agttttttgt
    10501 cagcaaatac catatcacac gagaatttaa aaagtacact ggatttacgt ttcatcagta
    10561 tgtaatgaag aaaaagctga tctatgccaa gcacttgttg cgagaatacc gcagctctag
    10621 tgctgtatat agtaaaagtg gtttttcttc ttatccgcat tttctgaaag cattcaagag
    10681 agagttcggc ataacgccta aagaattttt gaaacgtgat acacagaaac agttggttca
    10741 ttatgaacat tttgaagatg aacaatgaga atgatcacta aaaaataacc gcagtagctg
    10801 ctgttgttta catagaagca gaaactgcgg ttattttatt tttatctata tatttcaaaa
    10861 tattttgaaa aaatgacgta catattatat gaatcagctg aatttacgaa agtctctcgg
    10921 cgatagtcct gtttttgctt tgaacaggcg gctaaagtaa gcaaaattct caatccctat
    10981 ttcttcggaa atcattcgaa ccgtaaattc tgtataccga agaaggtctt tagcgtaaga
    11041 aagttttttg ctgatcaaat aatcgattgg tggagaaccg ataaatttgg aaaaatcttt
    11101 gctcagctga tatttattca ataagaattg ttgttctaat tgatcaagtg taatcgtttc
    11161 tttgaaatgt tcgtctaagt attgcttcag tttgtaaacg taggcgggaa tatcctcttc
    11221 ttcgaaatct agctgatact tttgcaaaat cagttcattc agcaactcgt ggatagctac
    11281 agaggactgg aaatcggtac gggcattcgt gccttgttgc atgtggatca attctgtgat
    11341 caaatggtgg atcttgtttt ctgatggatc ccctgttgaa tggaaggtgt tatggccgtc
    11401 ttttgtgaac tcctgataaa atgattggac gttctgacca ttcaaatgga tccaatccat
    11461 ttcccatggt tcttcgctga tcgtttgata acgctgatat tcttggcagt caataaaaaa
    11521 taagtctcca gcttttagct gttgctgatt atcattatat gtaagcgaac ctttgccaga
    11581 caaagtaaac tttatcaagt aagaaggtaa atgtgctcgt tcagtatagt aagggattgg
    11641 tgctttgaag taaccgattt cttggatata aaaaaataaa ttcctgcttc tttgtgtagg
    11701 cgtcaagatc actctttttg agtcttccga ccatgagtgg ttggcttttt cccagtaatc
    11761 catgtaaaca ctcccaactt gtcaagataa tgcaattata taccaaaata gtgtgaagca
    11821 aaatataaga ttctgtatta tgatagcatt gttaataaat gataacgctt tattaataaa
    11881 gtgctaatca acaataaagt aaaagaggtg gagaaatgaa tccaacattt gttgccatcg
    11941 atatcggtgc atcaagcgga cgactaatga aaagtacgag ggatgcaaaa ggttctttga
    12001 tcttggaaga agtacaccgg ttcaaaaacg gctttcggaa caaagacggt cacgatcgct
    12061 ggaagatcga ttatcttatc aaagagattt tgattggatt tgaaaaatta aagcagacag
    12121 ggattgatcg cctttttgcg gggatcgata catgggcagt cgattactgt ctgattgatg
    12181 aacaagggga tcggataaat gatccgattg cttatcggga caagcgtacc aatggagcag
    12241 tagaacagtt ttctgaaatc tattctctag ataagttgta tgagcagaca gggattcaga
    12301 tccagccttt caataccttg ttccaattgt ttgctgaaga aaaagaaaat ttggcaaaag
    12361 cacataaatt gttactgata cctgattatt tagggtatgt ctttactggt aacatggtga
    12421 cggagaaaac taatgcatct actatgcagc tgctgaatgc agggacaaaa gaatgggaaa
    12481 tagaacttct tgaaaaaatc ggtgtctcga aggaattatt tccaccattg acagagccgg
    12541 gaacgatact aggagatctg caaaaagaac aattttcaaa atatgatttg ccagatgtga
    12601 cctttatcac agtagcaagt catgatacgg cgtcagctgt attaggaacg ccaggtcaag
    12661 gagaacgttg gggttatatc agcagtggga catggtcgct tttagggatt gaaacaaaga
    12721 tcgccaatgt ttctcgcaat gctttttctg aaaattatac gaatgaatgg ggtgcccata
    12781 acacggtgcg ttttttgaaa aacatcatgg ggatgtggtt gatccaggaa gttgcccgaa
    12841 tgcaagagta cgagtacagt tatgcggaat tggcagagct tgctgcaaaa gaaaaaccgc
    12901 tgcagcaagt gatggaaatc aatgaccctc gatttttgaa tcccccaaat atgatagaag
    12961 agattcagca atactgtttt gaaacagagc agccagtccc tgtctcatcg ggagctatcg
    13021 ctcgttgtgt gtatgataat cttgctcttt gttacgcaca agagctgatc aaagtagaac
    13081 acttaactgg aacgaaattg gaaaagctgc acatcgtagg gggcggatcg aataatcgtc
    13141 tactgaatca attgacagca gattatgccg gaatcattgt agaagcagga cctagtgaag
    13201 ccacagcaat cgggaatgtt gccatgcaaa tgctcagtac aggtcagttt tcgaatatag
    13261 aacaagcaag aaatgtaatc aaacagtcgt ttccatgtga aactttttac ccagatgaaa
    13321 ataagcaaaa cacgatggaa tggtacgtga ataaaatcaa aggagtaatc gtatgaacac
    13381 gattgaaaga aattacgaac aagcaaaaga aaaatatgca actatcggtg tagatacaga
    13441 tgctgtacta gaaaagatac aggacatcaa aatttctatg cattgctggc aaggagatga
    13501 tgtcaaaggt ttcttgacac cggatggcga attgaccggt ggaatcatgg cgacagggaa
    13561 ttttccagga gccgcacgaa cacctgaaga gcttagacaa gatctggaga aggcttattc
    13621 gttgataccg ggcaaacaca aattgaacct tcatgctatc tatttggata cagaagaatc
    13681 tgtcgatcta aatgaaattg agccgaaaca ttttgaaaaa tgggtagagt gggcaaaaaa
    13741 agaagaaatc ggtcttgact tcaatccgac tttcttttcg catccaatga tgaaagacgg
    13801 ctttacattg gctcatccag ataaagaagt tcgtgaatac tggatcgaac acgggaaaag
    13861 aagccgcaaa atcgcggagt actttgggaa agaaactggg caaacaagta tcaataactt
    13921 ttgggtacct gatgggatga aagataatcc gattgatcgt tttaccccac gggaacgttt
    13981 aatggaatca ctcgatgaaa tttttacaga agagttagat gaacggtatg tacaagatgc
    14041 agtagaaagc aaactatttg gtttaggcgc tgaagcttat acagtcggtt cccatgaatt
    14101 ttatatgggt tacgggttga ctcgtgacaa acttgtttgt ctagatgctg gacattttca
    14161 tccaacagaa gtgatttcca ataaattatc ttcattagct ttattcagca aaggaatcat
    14221 gttgcatgtt tcccgtccag tgcgttggga ttcagaccat gtggttttga tggatgatga
    14281 attgcaggaa atcgctaaag aattagtccg taacgaattg ttggaaaaaa caaatatcgg
    14341 tctggatttc tttgatgcaa cgatcaaccg tatcgctgct tgggtcatcg gtacgagaaa
    14401 tacacaaaaa gcactgctga aagctatgct ggaaccagtt gagcgattga aagaaatgga
    14461 gctggctttt gactttactt caagaatggc ttatacggaa gaattgaaag atttcccgta
    14521 tgccgatgta tggaattatt tctgttacaa aaatcaagtt ccagttggtt tggattggct
    14581 ggaagaagtc caggaatatc aaaaagacgt gttggaatta agaaaatagg agtgtggcta
    14641 tattgaaaaa aatagatatt ttacaagcgc cttatgtgaa agaaatgatc gaaacgacgt
    14701 cgaatcttta tcgcttggga tgggatgaaa gaaatggcgg gaatatttct tatttactaa
    14761 aagaagaaga gatcacaccg ttcttggatc ctgaaaaagt gatccgccgc atctcgatga
    14821 tttttgatgc cagccgttta gcaggatgct attttattgt aactggttca ggaagatatt
    14881 ttaaaaatgt agcgagcgaa cctgccgaga atcttggaat cgttcgagtt gctgaagatg
    14941 gcaaaacact ggaacttttg tgggggctgg aaaacgaatg ccttcctaca agcgagttgc
    15001 caagtcattt catgagtcat atcgcccgtt tagaagtaga cccagataat cggatcgtta
    15061 tgcattgcca tgctagtcat ctattagcga tgagttttac gcatgaatta gacgaacgtt
    15121 cctttagtcg tacgctatgg caaatgtgta cggaatgctt agttgttttt ccagaaggaa
    15181 tttccatcat tccgtggatg gttccgggaa caaatgagat tggggaagca acagctgaaa
    15241 aaatgaaaga aactcgattg gttttatggc cacaacatgg aatctacggg tcaggaaaag
    15301 acatggatga agtctttggt ttgatcgaga cagcagaaaa ggcagcagaa gtctatacgt
    15361 acgtcaaagc gcaaggacca atcttgcaaa caatcacgga cgaaaacctg tggcatttag
    15421 cagatgcgtt tggtgtcact ccaaaagcag gttatttgga ggaagttcat acgaaagcag
    15481 gtgtctgaca tggtgaaaaa agctttttgc atgaaagttt acccagaaaa acaagaagaa
    15541 tacaaaaaaa gacatgatga actttggcca gaaatgcgta cagcacttcg tgaacacggg
    15601 gtcatcgact attcgatcta tcttgatccg agtacaagtt tgttatttgg ttacttagtg
    15661 atcgaagatg aaaagttatg ggaaaaaatg gcaaatacat ctatcaatca aaaatggtgg
    15721 aagtacatgg aagatatcat ggagacaaat ccagatgcct ctccagtcag ttgggaattg
    15781 atgccagtct ttgaactgta gcaacaagca actgggcaac cggttgcttg gggttctttc
    15841 gtttttattg tgaaaaaaaa tacaaattag gaggaataga aaatgagtaa tcgaatgatt
    15901 ttaaatgaaa catcgtattt tggaaaagga gcgattgagt cgatcgtccc tgaatttcag
    15961 cagcgaggat tcggcaaagc agctgtcatt actgacaaag gattgatcga gcacggtata
    16021 gcaacaaaag taacagaact tctagataaa gcagcaatcg cttatgcttt atacgacggg
    16081 atcgtaccta atccaacgat ccaaaatgta aaagatggag tggcttttgt taaagaggca
    16141 gaggcagact gtttgatcgc aatcggtgga ggttcgccaa tcgatacggc aaaagccatt
    16201 gggattattt tgacgaatcc agagttttca gacgtcatta gtttagaagg tgttgctgat
    16261 acgaaaaatc catgtttacc tatattagct ataccaacga cttctggaac agcagcagaa
    16321 gtaacgatca actacgtcat cacagatgaa gtaaatcacc gaaaatttgt ttgtgttgat
    16381 cctcatgata tcccaatcgt cgcgttcatc gacagcgata tgatgatggg aatgccgaaa
    16441 aaattggctg catcaacagg tatggatgct atgacgcatg ctattgaagg gtttatcaca
    16501 aaaggcgctt gggaaatgac tgatatgctg catttaaaag caatcgaaat catcggccat
    16561 tctttggaag tttctgtaga tggcgatcaa aatgggcgag aaaaaatggc attaggacag
    16621 tatattgcgg gaatgggctt ctcaaatgtc ggtttaggac tggtacacgg gatggctcac
    16681 ccgttaagtg catggtacaa cattcctcat ggcgtagcgt gtgcagcact tttgccaact
    16741 gtcatgaaat ataacaaaga atatactgga gaaaagtacc gtgagatcgc tcttgttcta
    16801 gggattaaag gagcagctga aatgtcttta gaagatgtaa gagaagcggc ttgtggtgag
    16861 atcgatcggt tgagcaaagc ggttggaatc ccagaaacca tttctgaatt aggtgtaaaa
    16921 gaagcagata ttccagcaat tgcagaagat gctttgcgag atgtatgcac accaggaaat
    16981 ccaagagaaa cgacagtaga agaaatcatt gctttatatc aaagtttgat gtaaaaacga
    17041 agcggatctt caacattaaa ttgcctgtaa agagaaatgg tagtttatat ctggatatct
    17101 cttttaaatt gatggtattt ccgctataat ttttcttata aagaaatagg agaatattat
    17161 atggaaaata aaaagcctta tggaactgta ttgatcaaag cctctaaaat actagactat
    17221 ttagcggaaa accaagatgt ctctttgcag acgatcgcaa agggtgtggg aatgacctct
    17281 tctacagtct tgaaaatact agatactttg ttgatgatcg gctacgtaaa taaaaattca
    17341 gagaaaaact atcgtttagg tgcaaagctt attcgctacg cgaataaaaa cattgaacaa
    17401 atcgatttgg tagagttgac tttaccgttt ttagaacagc tgcagcaaaa gatcgatgag
    17461 actatccact taggggtatt ggacaacaat gagatcttgt acgtgaacaa actagatcca
    17521 aaacatcaaa cgatccgcat gtcttcaaaa atcggaatca cccgaccatt atacaattct
    17581 gcgatgggca aagccgtatt agcagaattc agtgaagagc agtacactga ttatcttgaa
    17641 aagcatccgt tgatccctta cacagaatat acgatcacca atgcattacg gttgaaaaag
    17701 gaaatccaaa cggtaaaaga aacccacatc gcatttgacg atgaagaaat cgaacgtgat
    17761 attttttgta taggagcctc aatcgtgaaa aacgaacaaa tcattggtgc atttagtgta
    17821 agtatgccga aatatcgttt aacagatgag acaaaagaac aaataatcag tgccctcaag
    17881 caaacaaaaa gcgaaatcga gcaaagaatt caaaaataat gaaaaaagcg tttgcaattt
    17941 ttcttttctg ccgtataata tgggtataga tttccaagtt ataaaataaa atttctaaat
    18001 aggaaatagg tggagaagat gaaaaaaatt gatattttat ctcgattgaa aaacgccgga
    18061 gtgattgcgg tggtacgcgg aaaaagcaaa gaagaagctt tgaatgcttg ccacgcaatc
    18121 atcaaaggtg gtctaactgg tattgagttg acttttacag tccctcaagc agatcaagta
    18181 atcaaagaac ttttgtcatt ctacaaagac caaccagaaa tagtgattgg cgcaggcaca
    18241 gtattagacg ctgtcacagc tcgcttggct atcttagctg gcgcagagta tattgttagc
    18301 ccttcctttg atcaagaaac ggcagagatg tgcaatctgt atcaaattcc ttacttacca
    18361 ggatgtatga ctattaccga gatcaaaaca gcgctgaaaa gcggggtaga tatcgtcaaa
    18421 ctatttcctg gaagcgctta tggaccaagt atcatttctg ccttcaaggc gccgatgcct
    18481 caagtcaata tcatgccaac tggcggtgtg agtctggaca atatgaaaga gtggttcgat
    18541 gctggtgtcg taacagtcgg agtaggtggg aaccttttag cacccgcagc tacaggagat
    18601 tttgacaaag taacggaagt cgcgcaacag tatgcagcaa aaatgaaaga aatcaagagg
    18661 tagctgctta tgggaaaagt agtgacactc ggagaaatca tgttgcgatt gtccacagag
    18721 cagggaaccc gtatcgattc aagtgaacga ttcgctgccc attatggcgg aggggaagca
    18781 aacgtagcaa tttctttagc taattacgga catgaagcga tttttgctac aaaagtacct
    18841 gataacgcat tgggaaaagc agtcaaaaag cacttgcaat gttatggagt agatacgaaa
    18901 caggtattat ctggtggatc aagactaggt acttattata tggaaactgg tagtggcgaa
    18961 cgcgccgcct cagtgatcta tgatcgtgcc ggatcaagtt ttgctgaaat ggatcatttg
    19021 gaatggtcgc tagaggagtt attccaagga atagatatat ttcatgtttc aggaatcacc
    19081 ccagctttgt ctaaaaaatg gcaaaaaatg acgaaacagg tgatagaagc tgcaaaaaaa
    19141 gctggctgcc tgatcagctt cgatatcaac tatcgtggaa aattatggac acagcaagaa
    19201 gccagcgaag taatccatca gcttttacct ttagtagata tctgctcagc tggaaaaatg
    19261 gatgctcttt atttgttagg tatcgagaaa gcgccagaag cagaagaaca acctctaatt
    19321 tattattatc aaaaaatgca ggaaaagttc ccgaatattc aggtgtttta ttcgaccaag
    19381 cgtaccgttc attcagcaag tgaaaatgaa ttgaagggga cattgtggat gaaccatgaa
    19441 tatgtggaat cccaagtcca tagaatcacc ccaatcgtgg atcgagtagg tggcggagat
    19501 gcttttgcag gtggcgtgct ccatgggatt ttatccaata tgtctccgca agaaatcatt
    19561 gattttgcta cggcagcttc tgccttgaag catacgatcc atggggactg caaccaattt
    19621 agtcaagaag aagtcgcaca atttttagca tgcggctctg gaaaaatcat tcgatagaag
    19681 gagtagaaga aaatgcaaga aatggaaaca cgttatacac acagtccaga agatatccgt
    19741 cattattcaa cggaacaatt gagaaaagaa tttttagtag aaaaggtatt tgaaccaggg
    19801 aaaatcagtt tgacttatac gcacaacgat cgaatgatct ttggtggagt gacacctact
    19861 gaaaaagaat tagaaatcac attagacaaa gaactaggtg ttgattactt cttagagcgt
    19921 cgtgaactag gtgtgatcaa tatcggtgga ccaggattca tcgagatcga tgggcaaaaa
    19981 gaagaaatga aaaaacaaga cggatactat atcggaaaag aaacacgaca tgttgtattt
    20041 tcttctcaag atgctgaaaa cccagctaaa ttctatatta gttctgttcc agctcatcat
    20101 aaatatccaa atgtaaaaat cagtatcgac caagtaaaac caatggagac tggtgaaggc
    20161 ctgacactga atgaacgtaa aatctaccaa tacatccatc caaatgtatg cgaaagctgt
    20221 caattacaaa tgggttatac gattttagaa ccaggaagcg cttggaatac gatgccttgt
    20281 catacacatg aacgtcgtat ggaagcttat gtctactttg attatgccaa tgaagataca
    20341 cgtgtgttcc acatgatggg caaaccagat gaaacaaaac atttagttgt agacaatgaa
    20401 caagccatta tttcgccaag ctggtcgatc cattcaggtg taggtacaag caattactca
    20461 ttcatctggg caatgtgcgg agaaaatatc acctatacag acatggatat ggtgcaaatg
    20521 gatcaattaa aataagcata agggagagaa aatgatggaa tttagtatgg atatgtttcg
    20581 tttagatgga aaagtagcat tagtcaccgg tgctgtctat ggtattggat ttgagattgc
    20641 acgctcttta gctacagcag gagcaaaaat cgtatttaat aacttgaatc aagaatcagt
    20701 tgatgaaggt atagcgcatt ataaagaagc aggcatcgat gcaaaaggtt atgtctgcga
    20761 tgtgaccgat gaagaagcgg ttcaagcgat ggttcaacag atcaaagaag atgtaggttc
    20821 agttgatatt ctagtcaaca atgcagggat catcaaacgg acaccgatga tcgaaatgga
    20881 tgcggcagac ttccgccaag taatcgacgt ggatttaaat gccccattca tcgtttcaaa
    20941 agcagttatt ccggatatga tcgaaaaagg cggcggtaaa atcatcaata tctgctccat
    21001 gatgagtgaa ttaggacgcg aaacggtcag tgcttatgct gcggcaaaag gcggattaaa
    21061 aatgctgacg aaaaacatcg cttctgaata cggtcaatac aatatccaat gtaatggaat
    21121 tggaccaggt tatattgcta caccgcaaac tgctccatta cgcgaaacac aagaaaacgg
    21181 tgaacgtcat ccattcgatc aatttatcgt tggacgtacg ccagcagcac gctggggcga
    21241 gccaatcgat ttggctggac catctgtctt cttggcttct agcgcatctg atttcgttaa
    21301 cggccatgtc ttgtatgtgg acggaggcat acttgcctat atcggaaaac aaccttaaaa
    21361 gagctttagt acactgttta gtggagaggt tgtgaaatca gcggattgaa cgaataccga
    21421 aggacagctg attaaacatc atttattcga aaaaagaggc tgggacagaa gcatttaact
    21481 ccaagaaata agaaggaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcagctta
    21541 tttccgaagg agttgctttt acttccgccg tttgtacgtg tttagagcgt gagacaaaag
    21601 tgttctttac ttttgtccca cgctcttttc tgtgcgtctt ccatacttca ctaagtcgat
    21661 ccctcttgct tcgcaccaat ccttcactgg tccggaaaag atcagagatt gttggtgtag
    21721 ggccgcggta ttcgttgcgc cgaccatagt ataaagcaga cgaagctctt cttgccattg
    21781 cttcatcagt ataatagttt cttctacgcc gtgattcatc agatgcgtca gtacggttcc
    21841 tgaagtaccc acggcatttg cgccgagaca aagggcttta aatatgtcat aggcattgcg
    21901 gatacctccg gaggctagga tttccataga agtgtctgct tcatttgctt caagcaatga
    21961 agagactgtc gattgccccc agtcagctaa ataactcagt tcccgcttgg agcgacgggc
    22021 gttttcgatc tgggtgaagc ttgttccgct tcgaccgctg atatcaactg tatgaactcc
    22081 taaagcagct agatcattga gcgtttctct tgtcatgcca aaaccgactt cttttacgat
    22141 gagggggacg tcaatggctg tttgagtttc ttgaataagt actttccaat tggtaaaatc
    22201 acggtctccc tcaggcataa ccaactcttg aggtgcattc aaatgaatct gtaaggcatc
    22261 tgcgtgaaac aatcggatag cttctttggc tctttcgaca gaagtccctg caccgatgtt
    22321 ggcaatgatt ttcccgtgag gatattcttg tcgcataatc gtataagtat cggctaaaga
    22381 agggactttc aaggcagcgc tgactgaacc agtggcaatc atcaaatctg cttctcttgc
    22441 gacgattgca agatcttggt tgattttttt tgttttttcg cttccgccag tcatcgcatt
    22501 gatataaaat gggctgctta gtgtaaaccc aactgcctgt gtacttagat cgacatcaga
    22561 aactgctatt tgcggcaaag ggttatggat gatccgtacg aagtcaaatt cgtttttttg
    22621 tttatcatga aaggctttag ctagtgaaac atgttcgtct tttcgattca tcggttttcc
    22681 tttctttgat cgctatagac atgtaacggc agtggagtga tttctgcttt ttcccatgca
    22741 ctcattaaag gaagaatgcc agatttctgg tcaacaatta cgattccgca atcgccccca
    22801 cctgcaccag aagattttgc ggctccttca tactgttcag ctaaattaca caatttgttc
    22861 aaagcaggcg tttcgatgac gaccccagtg attgcagata aatcatgcag taattgtcgg
    22921 ttttttcgaa tcatctgttg gatcaacgtt acattatttt ctttaaaccc tttgatcatt
    22981 tcgttgacac attctgtact attttttaag aaaagctgat aagccaccat tttatcttct
    23041 ctcgaacggt gaacttgatc gaccaaatca gaagtagagg cagggctacc cgtccaacca
    23101 atcaataaac gtaaatcttc aggagcaatc aatggctcaa tggatagacc tggccaatcc
    23161 agggcaagta actcactgat agaatgctga gtttcttgtt cttggagcca aggatggtcg
    23221 aaggttgaga aagcgatcca gccaccataa cagctagcag cgatgtcgcc gcaggaacca
    23281 ttatcttgaa ctgctaaatt ggctagtgct gcaatcttga aaatctccaa ctgagataaa
    23341 ttcaacgcgt aaaaaacatt caaggctttg accgttgcaa cggtcacggc accgctagaa
    23401 cccaatccat atttccggcc atttgaactg tctaactcac ttgttacttt cagatcataa
    23461 aaggataaaa gaatgttttt ttcttgtgca tacttttcag tcaagcgaat agcagcaagg
    23521 atataatgaa aaggattttc ccgaatatct aaaactaatt ctccgttgcg tcttgtccaa
    23581 cgtacaggca tcccactata ttgtgcagat tggatacttc cgacttttcg tgcagattct
    23641 acagtgactg tcacgaattg atcgactgca gcgataactg ctggatggcc tgtttcaaca
    23701 actgcatatt ctccggcaat atagagtttg cctggtgcag atacttcaat cataaaaatc
    23761 tccctttatt caataatcgc aattcctgga ccagcatgtg cagtaattac ttgttctttg
    23821 ctaaataatt tggtaaatgt ttcttgtacc tcagataaat ggctgttttc cactaaaact
    23881 ttgacattag ggccagcatc catcgtaaag tagcatggaa tgccttgctt tctcaattgt
    23941 cgcaccgcat ccatcgcttt taagctgtca ggagaccagt aagtgaatgg aggctgggca
    24001 gctaaagtcg ttccatgcat ttttagtccg tttctttcca tcgtttctcc gagaagttgg
    24061 aaatcttttg ttttgattgc ttgtttcaat tgatatagat ccccttcaac ggaatctaac
    24121 cagccttgat aaaaactgga tgtttcgact gtccgacgca tcccatttct gctggacact
    24181 tcttttttct ggtcgttgat caaaacgaaa accattgcta aatcgtcttc gaaagaatcg
    24241 gaagggactg gcttagcgta agaacttaag tcgtcatggc ctttttccca ttcgacgaaa
    24301 ccaccgaaaa tacttcggca agctgaacca gacccgcgtc gagcaaatct tgaaagagag
    24361 agatcatcta atccaagctt aagagcagta ttgcaagcgc ctgctaaagc agctagcccg
    24421 ctggcagagg aggctaatcc agctgcggta ggaacgaaat tctgactgat tactgaagct
    24481 tttagtgaga ggccggcttc tttacgaaaa aggtcaagaa actgactgac tttttttgtt
    24541 gctttttcgt cttgcaaggt gccatctaga taaaattcat ctaccatata gctatcagaa
    24601 aagatgactt ctgtctctgt ataaaatgca tccaatgtta acgaaaggct gttgttcatt
    24661 ggaaggatga gttcttcatt tttcttaccc caatatttga ttagagcaat gttggtatat
    24721 gcgcgtgctt tgcctttaaa cataggtatg tactcctaat ccttgtatcc aagtttcagc
    24781 agcacccgca tcttcaagtg cttgactgat ttcttgggct tttgtttttg tttgtgctaa
    24841 tgcaatcata cagccaccgc gtccaccgcc agttaatttg gcacccaatg ccccggtatc
    24901 ttttgctgtt tggatcaata gattaagaaa atcattgctg attgtcagct tttccagaag
    24961 tgattgtgat tcatccattg tctgtgccaa cgtttctggt gaattttcaa tgattgcttg
    25021 ttttgcttgc ttcgtcaagt atcctaattt ttggatcatt tgtccagttt catgaggctg
    25081 ggtttcaaag aggtgagcga catctttgac ggcttctctt gtttgtcctt tgattcctgt
    25141 atcagcgaca atcaaaaaag catcgatgtt caaagaaaag tagtcgaaag gatgcccttt
    25201 tgtaaaataa atcggctgca agctgctagt agcggctgca tcgattccac taggattacc
    25261 gtgggcgatt ttttccgaaa gctggacatt ttctaatagt atttcacgag acaaaggaaa
    25321 tgctaagtaa tcataaaaag cacgagtgac tgctgtggcg actgcagcgc ttgatcccat
    25381 tccacgttca gcaggaatcg tgctttcaat ggtcagttgc aagttttttt ggatgtcatg
    25441 ctgttttttt aagtgaatga ataatttttt gatatttttt aatgcatgag gagcttcggc
    25501 taaatttcct gaatagtagg aagaaaccag ttgatcgtcc attgcatcca gctcttcaag
    25561 gaatgcggtg acttttgttg catagaaagg aaaggcaatc gctggttctc cataaacaac
    25621 cgcgtgctcg cccatcaata tgatctttcc gcttgattct ccttggccat agtttgccat
    25681 aatctaactc cttctcacgt aaaagtgatt tttaagcaaa aaataaagcg cccaaaacga
    25741 gttgcgctgc ttcacgaatt tttttgtcca ctaagggtct caaaaaacat tcctccttta
    25801 ttttactaaa accatctcag aagtaaaact aagaatacgt tttctaaaga aaaaacactt
    25861 ttttcttaag aattttttga ctttttttct tctttgacta gaaaaaaata caattgacac
    25921 tactagatat tgggggtata tttaaaaagg ttggagttaa acacaatatt tagtgatgaa
    25981 gtgagctgga ggtaaatgag cgtgatcgtt ttagcaggta caattggtgc cggaaaatca
    26041 agtttaacag aaatgatggc tgaacacttt gatagtcagg cattttatga atcgattgat
    26101 gataatgaag tcttgccctt attttatgca aaccctgaac agtatgcttt tttgctgcaa
    26161 atttattttt tgaataaacg ttttgcaagc atcaaacaag caatgaaaga tgacaataat
    26221 gtattggacc gttcgattta tgaagattca ctgcttttcc atctaaatgc agatctagga
    26281 agagcaacag aaacagaagt tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa
    26341 cttccctatg ctgcccataa aaaacatccg gatttattag ttcatattcg tgtatctttt
    26401 gatacgatgt tggagcgtat cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat
    26461 ccaagtctat acgattatta caaagaattg aacagacgtt acgatcaatg gtatgaagaa
    26521 tacaaagaaa gtccaaaaat ccaaatcgat ggagaccgct ataattttgt ggaagatcct
    26581 caagcaaaag aagaagtctt gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc
    26641 aaagtagcca tttgatttac agttgctcaa ataaagggta aacctttatt tgagcgactt
    26701 ttttctttat actggaaaca aatgtcagga ggatagagta tgcgggaaaa acactatttt
    26761 aatcaagacc gtaatttttg gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac
    26821 ttaaaagaag aatatggcat tagtgacgag atgctgactt attctctaga taaaaatgaa
    26881 cgggcgcgcg tagagtacga ttcttttgat gaagcgttac tgcttgtatc aaatgttccg
    26941 caccagcaaa aagtcgataa tcattatgaa actagtccta tcgcttttat tttaaaagaa
    27001 gacggtttgt ttacatttac cacgccgaat accgagtacg tgattcgact aattcgatcg
    27061 cttttggaac gaatgccaga catgagtgta tacagtttat tgtttcgtac attgtttttg
    27121 atttctgatt ccttttttcc tttaatcgag gaagtgaata gtgaacgtca gcggcttaat
    27181 ctgaaattaa gagaaaaaac aacgaacaag aatctgctgc aactttcaga tttagagatc
    27241 ggtttggttt acttagttac aggaacaaaa caaaatgtag tacttttaga acaaatcaag
    27301 gcattggcta tttatcgcaa gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg
    27361 atcgaagcac gacaagcagt tgaaatgacg aacttagctt cgcagatttt agaccagctc
    27421 tctggcactt ataataattt gctgaataac aacttgaatg acacgatgaa atttttgacg
    27481 gtctggtcgc tgatcttgac cgttccaact attgtgacag gattcttcgg aatgaatctc
    27541 cagttaccct ttacccattc cgttttcgga tggggaatcg ccttgatcat cagtttggtc
    27601 ttatctattt ggatgcttat tgcactgtgg cggcggattc gttgatctag tctttgatct
    27661 gcaaaccaag ggcttgggcg atcacaagtg cttgctcttg gttgacgatt agtcccgcca
    27721 aagaatcttg agataaagcg attgctgtga agaaattagt cgtaaagtct aatcccttca
    27781 gagaagttcg aaaccagttc gaaccggtca actgattgtc agacaaaaaa aggtttttcc
    27841 aagtgacctc ataaaattcg ctgtcttcta attgacaatg atcgaaggag acagctttca
    27901 agtttgtact actaaaggaa gccatcgaag caatacaatc agtgaaagta cagtctcgaa
    27961 gataactttc ggcaaaattc gtacctgtta atttacattg atgaaacacg acttggtgga
    28021 aactgccacc aatccactcc agattggaaa aatcacattt ttcaaaaatg acattactgc
    28081 attcaaaatg acgtaaaact gttttttgca tcgtaagttt ttggatatga ctctctctta
    28141 ataccatatt tttggcatcg agatagcttt ggtcaccccc gttgatcaga agaccagaga
    28201 agatctcctc atcttctaaa taaaactctc cctttgtaag aagaggaaga gaaggtggaa
    28261 taggttcgat ttttttcatg tgggttcacc tcgtttttct ttagtatagc aaagaagcgg
    28321 gaatcactgc tagaaagtct gctaatctaa tcattttcag aaaaggaaga caaactgtat
    28381 gaaaaacatt gacaaaaaga ttagtttttg ctatactatg atggttgaga aataaaagca
    28441 gaagcacccg cttctcgcct tagttgataa tgtcactggg ctagatagtt ttcactggtt
    28501 ctattaggtg aagtacgcgc gggttctgta agagaactcg tttttttatt gccgtttatt
    28561 cggtaagctt ttctttctct cataatattt ggaggtgaat gaccatagca aaggatatga
    28621 tggttaacga cggcattcgt gcacgagagt tacgattgat cggttcagat ggtgagcaat
    28681 taggagttaa gacaaaagca gaagcattga atattgcaga acaagcaaac ttggatcttg
    28741 tgttagttgc ccctggtgcg aaaccaccag ttgcgcgaat catggactac ggtaaatacc
    28801 gtttcgaaca acaaaagaaa gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa
    28861 aagaagttcg cttaagtcct acgatcgact tgaatgactt caatacaaaa cttcgtaatg
    28921 cacgtaagtt cttggaaaag ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg
    28981 ccattaccca taaagaaatt ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg
    29041 atattgcaac agtggaacaa aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg
    29101 caccgaaaaa cgacagtaag taatttgaat gactaactgg ctgttggaca gccgatagtc
    29161 ggataacgaa agattttgag gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg
    29221 atcagcaaaa cgtttcaaac gtactggtaa aggcggattg aaacgcttcc gcgcgtttac
    29281 aagtcaccgt ttccacggca aaacaaaaaa acaacgccgt caattgcgta aagcaagcat
    29341 ggtttctagt ggcgacttca aacgtattcg ccaacaacta gcaaaaatga aataagaagt
    29401 acaaataaca gaatctgaag atttattttt
//
LOCUS       DASJRQ010000024        28987 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJRQ010000024 DASJRQ010000000
VERSION     DASJRQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.024"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..716
                     /locus_tag="U4A80_000999"
     CDS             <1..716
                     /locus_tag="U4A80_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1974632.1"
                     /translation="GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLA
                     IDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKID
                     DQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELD
                     ATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILS
                     ILGVLLVFISIKFRKKRNI"
     gene            complement(1140..1310)
                     /locus_tag="U4A80_001000"
     CDS             complement(1140..1310)
                     /locus_tag="U4A80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1974633.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1504..1683)
                     /locus_tag="U4A80_001001"
     CDS             complement(1504..1683)
                     /locus_tag="U4A80_001001"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974634.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2070..3182
                     /locus_tag="U4A80_001002"
     CDS             2070..3182
                     /locus_tag="U4A80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1974635.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3260..3553)
                     /locus_tag="U4A80_001003"
     CDS             complement(3260..3553)
                     /locus_tag="U4A80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1974636.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3721..4035
                     /locus_tag="U4A80_001004"
     CDS             3721..4035
                     /locus_tag="U4A80_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1974637.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4056..6035
                     /locus_tag="U4A80_001005"
     CDS             4056..6035
                     /locus_tag="U4A80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1974638.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6048..6374
                     /locus_tag="U4A80_001006"
     CDS             6048..6374
                     /locus_tag="U4A80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1974639.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6390..6908
                     /locus_tag="U4A80_001007"
     CDS             6390..6908
                     /locus_tag="U4A80_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974640.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6908..8260
                     /gene="celB"
                     /locus_tag="U4A80_001008"
     CDS             6908..8260
                     /gene="celB"
                     /locus_tag="U4A80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1974641.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8344..9849)
                     /locus_tag="U4A80_001009"
     CDS             complement(8344..9849)
                     /locus_tag="U4A80_001009"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1974642.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9941..10531)
                     /locus_tag="U4A80_001010"
     CDS             complement(9941..10531)
                     /locus_tag="U4A80_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1974643.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10710..12356
                     /locus_tag="U4A80_001011"
     CDS             10710..12356
                     /locus_tag="U4A80_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1974644.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12405..13124
                     /locus_tag="U4A80_001012"
     CDS             12405..13124
                     /locus_tag="U4A80_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1974645.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13603..14550
                     /locus_tag="U4A80_001013"
     CDS             13603..14550
                     /locus_tag="U4A80_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1974646.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14562..15482
                     /locus_tag="U4A80_001014"
     CDS             14562..15482
                     /locus_tag="U4A80_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1974647.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15523..16980
                     /locus_tag="U4A80_001015"
     CDS             15523..16980
                     /locus_tag="U4A80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1974648.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17325..17939
                     /locus_tag="U4A80_001016"
     CDS             17325..17939
                     /locus_tag="U4A80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1974649.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17932..19656
                     /locus_tag="U4A80_001017"
     CDS             17932..19656
                     /locus_tag="U4A80_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1974650.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19653..21104
                     /locus_tag="U4A80_001018"
     CDS             19653..21104
                     /locus_tag="U4A80_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974651.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21577..23730)
                     /locus_tag="U4A80_001019"
     CDS             complement(21577..23730)
                     /locus_tag="U4A80_001019"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1974652.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23732..24778)
                     /locus_tag="U4A80_001020"
     CDS             complement(23732..24778)
                     /locus_tag="U4A80_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1974653.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24928..26217
                     /locus_tag="U4A80_001021"
     CDS             24928..26217
                     /locus_tag="U4A80_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1974654.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26230..28932
                     /locus_tag="U4A80_001022"
     CDS             26230..28932
                     /locus_tag="U4A80_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1974655.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9422 a         5009 c         5871 g         8685 t
ORIGIN      
        1 caggcggtgt cgtgttgaca aagatcgatg accaaagcgg ggaagtcctg caaggagctg
       61 tttttgagtt acaagatgcc aatggaaccg ttctgcagtc aggtctaacc acaggagcag
      121 atggaaaact ggcgattgac ggcttagcac caggagtgta tcagctggta gaaacccaag
      181 cgccaacggg gtatgaattg gatgcgacac caatcgagtt tgagatcgaa agaagtcaga
      241 cagcggtggt agaattgacg aaagaaaatc gacttacacc aggcggtgtc gtgttgacaa
      301 agatcgatga ccaaagcgga gaagtcctgc aaggagctgt ttttgagtta caagatgcca
      361 atggaaccgt tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgatagatg
      421 gcttagcacc aggagcatat cagctggtag aaactcaagc gccaacgggg tatgaattgg
      481 atgcgacacc agtaactttc aagatcgaaa aagaacaaga agtggcgatc tttttaacaa
      541 aagagaatag aaaagttgct gatagctcag atataagagg aaacaatcca acaagcagtg
      601 gaaataagca tcttccaaaa actggagaaa ccatcattag ccagtttata ttgtcaatat
      661 tgggtgtgct acttgttttc atcagtataa aatttagaaa gaaacgaaat atatgagcta
      721 aaataaaaaa ttgaataagc atagttttta aaatcaacta gtacccatca attgaatgga
      781 aactaccgat tttagaaggg gtatacaaac aaatattagt atcggagtag agaaggttga
      841 acccaaccag taaataagta aagacaattt tgtgctgcgt tgagaagtga aaaataaacc
      901 tttgtagtaa tactagttaa taaaaaatat acaaaaacac tgcgaaaatc attcttgagt
      961 ttcgcagtgt tttttgccta atgactcaag tttattgggt aatactttga ttttctttct
     1021 acaaataatc agcgatcagt tgtaattttt tctctagttt taatggctct ttgtcaataa
     1081 ggagaaaaac aagtcgggtc ttttgtctcg gaaagcttca accaactcgt gaataaaggt
     1141 tacgcctcct tgaaaggcgt ggaaaaacca agtaatcgat caatcaccaa tttcgcattt
     1201 ggtagaacat gtttggtgag ctgaaagtag gtggcgttca tgtctgtaac taagaattct
     1261 atttctttag gattagtgca gctaaggaaa tagtttgtta atcgaggaat tttttgtgta
     1321 ggtaaatttt ctcgattaat ttttctgtct cgccatccgc ggaaataaaa ctcagtttat
     1381 cctcaacgga aacgtgagaa cgaaattcac aaccctcaac actcaagaca gaattctttt
     1441 ggaagacttt ggtaaatagc ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga
     1501 tacctaacaa cttttcgaaa taaaaaataa cgacactttt tcagtcagta aagaagtgat
     1561 tttatgtctg acatgatttg aaatcgagtg ccttggacgg ataaaataac tttgagcgtt
     1621 ccaatggctt cgacagtttt tacaggtata gcgctgcttt tttaggcgta taaccattgg
     1681 catatgatta tattattcaa agtggacaat ggtttccttc ttcttgtttt tcaccaccat
     1741 taatttccta tttccatcta ccacagcaga accacaattt ctacagacac atggagcagg
     1801 gaagagaact gcagcaacga tcaatgtttt tttcttctgt aaggtttcgt aagagacctc
     1861 tgtaatcatc aaatttttgt ctattgatct cagaattttt ttgatagaac cattcataaa
     1921 gcatatcgtt ctctcagttg tttattttgt ggtgatttaa tcatacaaga aaacgatatg
     1981 tttttttata aaaacatgaa taggaaaaat agttgacaag aaaatgacaa cgccatatga
     2041 tgttccatag tatttacaac ctgtcataga tgactgtaaa aaataaaagt aaaaagaaag
     2101 gacgacaaca ggtggatttt tatcagtctc tacaactaga tccgtttatt ttaaaacaaa
     2161 aaatacgaga agcagcttct aaaaaagaaa agtgcatgta tatttgttca ttgtttttac
     2221 gatcattatt catcgtattg tttgccattt gttttatcat tataattaca acattatttg
     2281 aatcgaagca taaaccatac gctgtggttt tattctgtat gttgatgagt attcgttttg
     2341 ttgactttgg ttataagatt tcccattcga tcataggttt agcaatcgtc atgttctcac
     2401 tattagtagc accgtatgtc cagctgatca aatggtcagt tatgggcatg ttgattcatt
     2461 ttattttact aagcagcata ttaatggcaa ccgctagtga tcccaaaatg gggaatgctt
     2521 ctttgtacgg cttttcatat ctctttattg tttattcgtt acctaaagat tcgttgaaca
     2581 agaatttctt tacacaaaca agtagcttat tatttttgtt tttttgttgg ttttcagttc
     2641 tcctttacag aaaacatcga gaaaagaaca aagacaaaag cttgtttaaa gagatttttt
     2701 tgaaaggcat gtattcgcag gaaaaaattt ggatgctcat ctatgcattt ggcattagtc
     2761 ttttgatcgt tgcaggagag tatgtccctt ttcagcgatt gatgtgggca gggtttgcat
     2821 tttcttctat tgtatctagc tatggattga tgtcgattgg tttcaaagaa cgagcagtag
     2881 accgaatcat tggctcattg attggatgtg ttttatttat tggtatcagc caatttattc
     2941 cttttaactg ggtcggtata ttaggtggtc tagctttagg tgtttgttct acctatagat
     3001 ataaaacagt ttttaattct tttggtgctt tggcaatcac tgccagttta ttcggggttc
     3061 caggagcagt aacgatccgc atttttgaga atatccttgg ggtatgttta ggaatcatgt
     3121 atattggggt gactgaaata cttatccgaa aaataagaga aaaacacggt ttgaatcatt
     3181 agatgagatc gaataaaaaa taaggaaaaa gtgaagatac acatggatga taaattgcta
     3241 tcattccatg tgtatctttt tacggtaaat cagtgaaacg ttcgactttc atttttaatt
     3301 tatacttttt acggagttcg tttatttgag ccatcatttc ggatttgtga tgcttttcga
     3361 ttgcttcttc atctttccaa cggtcgatca gtaaaagaga ttctggatct tctagtggaa
     3421 agtaatagtc atatttctcg tttccttctt ctgcacgaat cgctttgaca atgtttcttt
     3481 cgaccatttc ttctgcaaat tttctggctt gtccatttgt acttgtatac aaaatattca
     3541 ctgtgatact cactaaatcc cctcctgaaa aaattctatc actagtatat cacggtgcca
     3601 gaaattatta ttcaagatat gatacacatt ttttccgcaa tgctacggaa atatatattt
     3661 tgaatagaaa acgcttacaa gatatgcttg gtttataaaa ataaaaggag tgattgattt
     3721 atgaaaaaag cattgattat ttgtgcggca ggaatgtcat cctcgatgat ggcctcaaag
     3781 acaacggatt tctttaagca aaaaagagaa gaggtactcg tagatgcagt ttcagctaca
     3841 gaaggcgata atatgattaa gaatagtgat tttgatttat ttctgatcag cccgcaaacg
     3901 acgatgtttt tggataaatt caaaaaatta ggagaatctg ttggaaagcc tgttgttagt
     3961 atccctttcc aagcgtatgt cccaatccct acaggcatcc agaagttagc agaggtgatc
     4021 gaagagaaca tatgatggaa taggagtgga tgataatgaa acagaaggaa aaattgctgt
     4081 tgagatattt gattgatcac aagaaggagt atgtcaccag tcaacgttta gcttctgaat
     4141 tgttattatc tgatcggaca atccgtaatt acttgcagcg gatcaaagag ttagtagaaa
     4201 aaaacggagg taagatcatt gctaaaccag gttatggcta ccaactgcat attttacaaa
     4261 gactgacatt tgacttgttt ttgagtcggc aagaaatagt acctggatat gctagagaac
     4321 cgcaaggatt ttatgagtcc gaagatcgac aaaagtatat attgaaccaa ttgcttttgg
     4381 aagatgccat tctttttatg gatgatttgg cagaagagct ttatatcagt cgttccagtt
     4441 tgacaaagga catgcaagaa atcaaggagc gattgattcc atattcacta aaaatcgttt
     4501 ctaagcatgg tcaagggaca tggatcgaag gggaagaaag aaatcgacgg cattttatca
     4561 tggatacttt ttttggaaac cattatggaa attcattgaa agaatattta gggcaaagcc
     4621 gttttttccc agacatcagt tttgaagagc tagtcattat cattctcgat gaaacaagag
     4681 aagctaagtt aaaagtttcg gattttatca tacagaatct gactttacac ttggctttgg
     4741 ggatcaaacg gctccgagaa gggtttgaaa tcaaggaatt aggcatcgaa caagaagttt
     4801 ctaagcgagt agagtatcaa gtggcgcagc ggatcgttca acgtattgag gtaatcgcag
     4861 gtgtatcgtt tcccgaagaa gaaattgctt atttgacatt acatctcatg gctaagtcga
     4921 atcacaaaga aaatcaaaat gatcaggaac tatcaaagga attagcgaaa gttctgaaaa
     4981 gattgtctca ggaatttcat ttttcattag tagaggatct ccagcttttc aatggactac
     5041 tcgatcattt gtcaccgatg atgattcgat tgagccgagg tattcgcatt gagaacccat
     5101 tgactgagga gataaagaaa gaaaatccca aagtctttga cgcagtaaaa aggcatttca
     5161 gcaacatgcc ggctttgaaa aactatacca tcaatgaaga tgaatgggcc tatttggctt
     5221 tgcatctcat ggcggcattg gaaaaagaaa gagctgcgca taaactacat gcattgatta
     5281 tctgtgcaac gggttacggc agtgcccaac tattaaagaa tcgcgtagtc agtgaattcg
     5341 gtaaaaatat tacagtggta agtgtaaaag gctattatga gatcaatgaa acaactttaa
     5401 aaggaatcga cctgattatt tcttctattg atctttcgac aatgtttttc aaaattccag
     5461 ttttacatgt cagtattttt ttgaatgaag atgatgttcg taaaatacga aaggtagttg
     5521 gagaatctat accctatcat tcctcagata gaccgatacc agcattttct ctacagcaaa
     5581 aaaagcagat ttacaccgaa cagttgtctg aaaaattttt caagacttat acgtacgaac
     5641 cgaaaaagga agaaattctt catgatttgc tgcaaaagct gagcataaat gaagaagaag
     5701 gatatataag agaaatgcga aagcagatcc agcaacgtga aaatatggga caaatcatat
     5761 tcagtgatac agtggcagtc ccacatcccg ctgttccagt tggaatcgaa acaaaggtag
     5821 ccgttgcatt gattccttct gggatgcagt gggatcaata tcaagaaatt cattttgttt
     5881 ttttagtttc gccttcttat gtcgaaaatg aaggaattac ggtcgtgact aaagcaatcg
     5941 ttaggttagt tgaccagcta aaagtacagc aggaaatctt agctgaacct atttttgaga
     6001 atttcagcag taaatttatc aaactgatcg aataaggagg aaaacagatg gctgaaaaaa
     6061 taaacagtga aacactacaa gtgacttctt ttgatatcat tttccatagc ggaaatgcac
     6121 ggacactgat acatgaagca tttcgctcga tgcgtaaaga gatgtttgaa gaagtagaag
     6181 agaagttgcg acaagcaaat gaagagattc ttcatgcgca tcagtcacaa actcaacttc
     6241 tgaaagaata tgccagcggg acaaaaatag aaatggaaat tatcatggtc catgcacaag
     6301 atcatctgat gacgacgatg acattattgg aagttgcaca ggaaatgaat tatttgtatc
     6361 aaaaaaataa atagaaagga aggatcattg tgcaggatac agtaaaaagg aaaatcgaat
     6421 tagaaaaaaa gcaacttagt ttgaaaaaca tgtatttcaa tcgttatcta atcgtacgtt
     6481 acctgactgc ctttttcttt ttcatgaaca tgcaatggat gattctgctt ctatcagcta
     6541 aaagtttagg aagtagttta ccgatgattc tgattttagc tattttgcct gcagtaggag
     6601 aacaagtaaa gctttatcga aaacaccaaa cgaacgttcc atggacaaag tggtattttt
     6661 tgttccagag tgtctttaat ttattagtga ttccagtgtt gtttacctca ggatttacat
     6721 tcttgtatcc ttttatggca aataacaatg gaggacagtt atttgttttc attcttgttg
     6781 taagcggtat ttttgccagt gcatttatac agtatcgttt aaagaaaatt tcactcaacc
     6841 aagaccaaca gtatatccgt attaaacaat atgaaaaagc gttatattta ggaaaggaga
     6901 ataactaatg tttgattttt tgcagaaata cctgatggga cctatgggga aaattgcgca
     6961 acttaaaatc gttcgggctg tcatggcagc ggggatggct tctattcctt tcacgattgt
     7021 aggctcgatg tttttggttt taaatatttt gccattaccg ttcccatttt tagaaggatt
     7081 ttttaatgca acttttttca aagttagtga cttgtacatg atcgtcaata caatgacgat
     7141 ggggatttta tctatttact ttgccattgt gtttgcttat gaattaacgt caattgaacg
     7201 agatgaacaa aaattaaatg ttaatccgct cacgggggca ttactatctg tttttgcatt
     7261 ctttatgtgt atccctgaat tgatcatgac tgatggtaaa atcagcttga ttgcttctat
     7321 caatgagaca gaaactgtag taaatggtat taggatgggc tcttttgtcg aacgtttagg
     7381 aacttcagga atctttacag ctattattat gtcgattatt gcggtcgaat tgtattgtat
     7441 gtgtgtcaag agaaattggg tcataaaaat gccagatgtt gttccgccag gagtttctcg
     7501 ttctttcact gctctgatcc caacatttgt cattgcgttt gtggtgatga tcatcaatgg
     7561 attattagtt gcactaggaa cggatatctt caaaatgatt gctatcccat ttgggtttgt
     7621 tacgcagttg acgaatacat ggcttggtgt catggtgatt tatttcttga tccatgcatt
     7681 gtggatcgta gggatccatg gagcgaatat catcacttct ttcttgaccc cgatcgtatt
     7741 agctaatatg gcagctaacg cacaaggcgc aaattatccg ttggcaggag aattcaacaa
     7801 ttcttatgta accgttggtg gatcaggtgc gactttagga ttgatcatct ttattgcatt
     7861 cctcgcgaaa tctgatcagc tgaaagtgtt aggaaaagcc tcacttgtac caggattatt
     7921 caatatcaat gaaccaatca ttttcgggat gccaatcgtc tacaatccct atttagcggt
     7981 tccgtttttc ttagctccga tggcttctgc tacactcgct tattttgcca ttcggctgga
     8041 aatcgtcaaa ccgatgcttg cacagatgcc gtggccttca ccagtcggga tcggagcatt
     8101 tgttggtagt ggtggtgatt ggaaagctgc tgtgttagca gtcatttgtg caatcatcgc
     8161 attcattatc tggttcccat ttatcaaatt ctatgataat aaattattaa atgaagaaag
     8221 agaaaaggca gcagaattag ctagtgaaaa gttaaaatga tttctaagaa aaaaggaact
     8281 gtgacaaaaa atcacagtcc ctttttttct tagaaatggg aaaccactag tatataaata
     8341 ccattattct tcaatcaacc gcttcacgat tgcataatcc ccttcataag gttcatctac
     8401 accattgatt ttttgatagg gatccacgcc ttttcccgca ataacgactg aatcttgcgg
     8461 agcgctttct gctaaagcgc gtcgaatcgc ttcaggacga tccatttcat aatgaacgat
     8521 caaatctgga ttagtaattg cttcattgat ttcttccgcg atttttttcg gatcttcaaa
     8581 tgctgggtca tctgccgtca aaaaggcgat gtcagctgtt tctgatagca cttttccaaa
     8641 atcatgtcga cgggagattg ctttgtttcc tgggctgcct aatacaacaa ttacacggcc
     8701 atctggatgt tcatttttgg caaatttcag caatgttttc aagcttagat aattatgtgc
     8761 atagtccaca tagacatgcg ctccattgga ttggatcaat tgatccattc ggcctggtac
     8821 acgagcttct tttaatccgt cttgtgcatc ttgactggag gcacctgtta gtgctgcggc
     8881 aatgatcgca ctggctgcat tttcttggtt gaatcctcca gccagcaaaa tatcataagc
     8941 agtatcgcag atagctaagc gatcttcatg agaagataaa gtaaaaccgt gagtaccttt
     9001 atctgaacgg atcacttgat aattgctttc ttccgaacga ccataagtga tcgttggaat
     9061 ctggaataga tcggctgttt ccctaagtaa atcaaaatag tcactttcat gatttaaaac
     9121 aactgttttt gaattcaaga tcaattgtcg cttgcaatag aaatagtcat caaatgtcgg
     9181 atgttcgatc ggactgatat ggtcaggcga gatattcaag aagattccga catctaaagt
     9241 caagccgtat acgcgttgtg ttttgtaagc ttgtgaagaa acttccatca ctagatgagt
     9301 cataccattt tcaactgctt ctgacatcat acgatataaa tctaatgact caggcgttgt
     9361 caaatgggat ttgaaatatg ttttgccatc taacgttgta ttcatagtag aaagcaatgc
     9421 tgttttttta tttgttgtgt gatccaagat tgcttttgta aagtacgcag cagttgtttt
     9481 accttttgtt ccagtgaatc cgatcacttt caatttattt tgcggatagt cgtaaaaagc
     9541 catagcaaga agcgccattg ctttacggat atccgttacg atgattccta ctgctgaatt
     9601 ttcgtatggt tgttctgata cataatattg aataccctgt tcgattgctg aatccaaata
     9661 ttcttctttg aaattcattc ctttacagaa aaacaaagta gaagcatcag cttttcttga
     9721 atcataactt aactgaagaa attcagcttt ttctggtaca tcaagatgcc agcctcttgt
     9781 tgaaacaaat tcttttaata gattttcttt taatagtaaa tgacggatgt cttctaaaga
     9841 gaagctcatt gattgatccc tcgctttatt caatttcttg gtttattata gcacactctt
     9901 tttaatatat cgtttgtttc cgctatgaca aaatccagct ttattcaaat tttcttgaca
     9961 caggggacaa gcggatgagt attttcataa acaatgaact gactgtaata gataggagaa
    10021 cgaatagtgt ctccaacaat ccaaaaacat aataattcaa aaagacgata agactattgg
    10081 taagtaaaag tccttgcggc attggaagcc gaaaaaattt gtggaaaata atcggcggta
    10141 tcgttatacc gccatttgag gccccgactg tgtacaatgt gtaaacccca agactgaaca
    10201 aaagacttcc tgaaatcagt gaaaagaaat gtgaggagat catttccact tttggaacaa
    10261 tctctaaaac aacaggaaaa agcagacttc ccgctacagt cttaaaaaat actgattttc
    10321 ctaataggac agcggctaga agcagcatca taagattgat gctccaaaca gtaactgagc
    10381 gttggataga aaaggccgtt tcaactaaat gtccgatggc gcccactccg gcggaagcta
    10441 tatcgtgagg gagcaagaac caattcaatc caactgtgat aaagaaaaga cctaaaaata
    10501 taacgggtag tttttttatt tcttgtgtca ttgatatctc ctataaatgt tagccataaa
    10561 aggctgtata tttctttact ttaccaaaaa aacgaaaccg aattcaaaaa aacaattgtt
    10621 cttataaaag ttatataaaa tcgattgcca gtttctgttt taccaattaa aatgatagta
    10681 atgttttgta gaaagttgga atctgaccga tggataatca aagaaaacca gcattagatc
    10741 aattaacacc gcaagaaaaa atggcacgag gctctgcttg gatgaccgcc agtaatatca
    10801 tttcaagatt gctaggcgct gtctatatca ttccctggta tgcttggatg ggcgaaaatg
    10861 ctaaggctgc caatggatta ttcaatatgg gatacaatat atatgcattg tttctactga
    10921 tctctacagc aggaatacct gctgcgattg caaaacaaac tgcacgctat aattcattga
    10981 atgaatatgg cacttcacga cgattattta tccgtgcatt acagatgatg ggcgggttag
    11041 gcctgttgtt tgctttgttc atgtacatcg cctctccttg gctagctcat gcttctggtg
    11101 gcggagaaga actgattcca acgatgcgtt ctttgagtat cgctgtattg gtttttcctt
    11161 gtatgagtgt gatacgaggg tatttgcaag gcaatcaaga aatgatgcct tatgcgctgt
    11221 cgcagattgt agaacaggtg gcacgtattt tttatatgct attggctaca ttcatcatca
    11281 tgaaagtcat ggatggtgac tatgtgacgg cggtcacgca atcaactttc gcggcattta
    11341 tcggtatgct agcaagtatt cttgtccttt tgtatttttt gaagaaacat caagcgtata
    11401 catcggcgtt tattcactac agcgaaaaca aaataaatat tgcaacgaag gaattgttgt
    11461 tagataccgt taaagaagcg attccgttta ttattgtggg ttcaggggta acgatcttca
    11521 aattagtcga tcagttcact tttatgaaga tcatgtctgc atccaccgag tattccaatg
    11581 cacagttact ggatcttttt tcgattttta gtgccaatcc agataagttg acgatggtag
    11641 ttattgcatt ggcaacatcg atcgcgtcta ctgggttacc gttgattaca gaagctgtta
    11701 cgttgaaaga tcgagtaggt ctggcaaaat tgacaagcgg aaatttacag ttgttttcat
    11761 tttttatgtt tccggcgaca tttggtgtta tgttacttgc ctatccgcta aatacattat
    11821 tttatacacc ggattctcta ggaagtaatg tactgatcca agcaagtttt gtaggactct
    11881 tcctaggact atatatgcta gtttccaaca tgctgcaagg aatgtttgaa aacaaggcgg
    11941 caattaatta tttggttatt gggtttctag tgaaactcct tcttcaatac ccagccattc
    12001 gtttatttga agtatacggc cctttattgg cgacaatgat cggatttgct gtgtcttgta
    12061 ccttgatctt aaagagaatc catgaagtag cccgctttaa accaagtttt gtctggcgca
    12121 ggacattgct gatctttatt cttaccttga ttatgctttt ggcttcgtgg atcacaaaga
    12181 tgatttttgg tgctttttta aacgaagata gcaaattcca gtctctattg ttgattcttg
    12241 tagtagcagg tataggggct ttagtgtatg gttatttagc tttgaaaatc cgcttggcag
    12301 aaagactact aggaccagga atagcgcgtt tacgacataa attaaaaata aaataagtga
    12361 atcagaagag ctagattgat gctcttttga aatgggggaa acgcatgcgt ttagataagt
    12421 ttttagcaga tatgggactt ggcagccgca aagaagtcaa aggattaatc aaaaaaggaa
    12481 cgatcgaagt caatggacaa gtggtgaaaa gcgataaagc gcaagtaaat gaattcgaag
    12541 atgcggttac ttatttagga gaaatcatta cgtatcaaaa agatttttac tacattttgc
    12601 ataaacctgc aggagtcatt tctgctactc aggataatca tgatcaaacc gtaatcgatt
    12661 tattggaaga ccaggattat cgggaagatc tttttccagt cggccgatta gataaagaca
    12721 cagagggcct cttgatttta acgaatgacg gggtgtttgc ccatcagtta ctatcaccta
    12781 aaaaacatgt ggagaaagaa tatctagctg aggttagcgg tataatgaca gaagaagata
    12841 tccgtttatt tgcagaagga ctgattattg atggcgatga gcagacattg ccggcaaaac
    12901 tccaaattga tcaagtagat caagagacag aaacttcaat tatccgtctg attttgcatg
    12961 aaggaaagtt tcaccaagtt aagcgaatgg tcaaagcagt aggaaaagaa gtcttatatt
    13021 taaaaagaat ccgtatggga aaattttggc tgcctaaaga tttgaataaa gggcaatatc
    13081 gacccatgac caaagaagag ctagaacaag tgagagaaaa ataattgaat ggatagctaa
    13141 aagaataagc agagtagttg tagcgccatc tgaaaagcag acttgtccgt ctgactttcg
    13201 gatggctttt ttgtgaaatt tcggcttatt tccgtagaag ctacttctgg aacaccgttt
    13261 gttcggaata agggactgtg acaagacttt tgttacagtc cccattgttc cttgtgactc
    13321 agagttagat agtaaggaca cgatctccct aatactcagg ccaaaacgct gaattcacaa
    13381 cctctgataa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    13441 atgaggaact attttcccgt ttttttctct aatactcagg ccaaaacgct gatttcacaa
    13501 cctctcctat actttgtttc attttcccta tactttgttt atttattctc attattgaaa
    13561 gcgctataat tacgttgcga atgaatcgaa aggggttttt agatgaaaaa acgaaagggg
    13621 ttcaatcatt tttttatgaa tctatggcgt tataaagcac ttgtcctgat ggctgtaccg
    13681 ggtatgcttt ggatgatttt tttcttttac atacctgtaa tggcgaatgt cgtagcattc
    13741 aaaaattttc atatttcagc agatgggttc ttggctagct tgagggaaag tccttgggtg
    13801 ggttgggaaa atttcaaatt tttattttct tcggatcaag catttctcat cactaaaaat
    13861 acaattttat ataatgtagc atttatcctt ttgaatctgc tgatctcagt cgtatttgca
    13921 atcattatga gcgagttgag aaataaacga ctcgttaaag tttatcaaac gatgtcctta
    13981 ctaccttatt ttctatcttg ggtcattatc agttactttg tttacgcttt tttaagtcct
    14041 gacaaaggaa tattcaatca gtggatcact ggacatggag gagaagctgt caattggtat
    14101 aatgaaccga aatattggcc gtttatcttg gtgttcattg gtacatggaa agggattggt
    14161 tataacagca ttatttactt tgcatctgtt atgggaatcg atccgactta ttatgaagct
    14221 gctatggttg atggagctag caaatggcag cagatcaaac atgtgacgat accacaactt
    14281 attccactaa tgacgatttt aacgatttta gccgtaggaa acattttccg agctgatttt
    14341 ggcctgtttt ataacattcc taggaattcc ggtgcattat atgatgtgac tcaagttttg
    14401 gatacgtata tctacaatgg cctgacatcc acaggagatt ttggtatgac ggctgctgca
    14461 ggactttacc aatcagttgt cggctttgtc ttgttgatga tcacaaatac gatcgcccga
    14521 cgttttgata gcgattctgc attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg
    14581 agagagtcca agtccgctct ttcaataaga cggctaattt tttctctaat ataatgattg
    14641 cactctttgc tatttcctgt attttgccat ttatatttgt tatcgccatc tcttttaccg
    14701 atgagtccat attgtcgact cacgggtaca gtttttggcc agaaaagttc agtatatttg
    14761 gttatacgta cttgttcgac cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg
    14821 tacttgttac tgttttaggt acactgatca atagcacggc tacttcttta tatgcttatg
    14881 cgatttcacg gtcaaacttt ccattccgtc gtttttttac cgttttttgt ttgattacga
    14941 tgttgttctc accagggatg gtggccaatt atcttgttat gacaaatctt cttcagttaa
    15001 aagatacgat ttgggcattg atcttgccaa tggctgtcag tcccttcaat attattgtga
    15061 tgcgcacatt ctttaaacgt tcggtctctg attcgattat tgaatcggca cggatcgatg
    15121 gagctagcga actgcgaata tttgttcaga tcgttttacc gttagctgtt ccaggtattg
    15181 cgacaatcag tttatttgcc gcattgggtt actggaatga ctggttcaat gctttactat
    15241 acatccaaga tgataagcta gttccactac agtatctttt gatgaaaatc caaagtaata
    15301 ttcaatatct gacacaaaat tcaggagcag gaagccaatt gacaggcgga ttagcctcag
    15361 ttccaggaga atctgccaga atggcgatcg tagtcatctc gacgttgcct attgcgatca
    15421 gctatccatt tttccagaag cattttgtca aaggtctaac aatcggcgga gtaaaagaat
    15481 aaagaaacaa aaatataaat cataaaatta ggaggaagaa aaatgaagtt atggaagaaa
    15541 ctagccttta caggtgtttc agcgttgttg ttgggaacat tagcagcatg cggaggttct
    15601 ggttctaaag accaggcaga agctagcaat tcggacactt tgcagatgta tcaaatcgga
    15661 gataaaccag ataatttcga tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag
    15721 atcggtgtaa aagtcaatat caattacatt ggctggggcg attacgaaaa gaaaatgaac
    15781 gtcatcattt cttccgggga aaattatgat atcgcctttg cgaacaacta tgtttccaat
    15841 gcccaaaaag gagcctttgc tgatttgact gaattagcgc ctaagtacgc tgaaaaagct
    15901 tataatgatt tagatgaagc atatattaaa ggaaatctgg tcaatggaaa attatatgct
    15961 ttcccagtca atggaaatgt ctttgctcaa caagtactta cttttaacaa agctttacta
    16021 gataaatacg atctttcaat tgatggaatc caatcttacg ctgatgcaga aaaagtcttg
    16081 aaggaattcc atgaaaaaga accaaacacg gcagcttttg ccattggaca aggcttcaaa
    16141 gtccaaggga actttgatta tcctttaggg aatacgttac catttgctat tgatctggat
    16201 ggagatgatt cgaaaatcat caaccaatac gataatgaaa cgtatatcgg actgttacgt
    16261 acaatgcata gctggtacaa acaaggactc attccaagtg acgctgcaac gagcaataag
    16321 gattatccac tagaaggaaa tacttggcta atgcgtggag aaacacaagg accgtatgat
    16381 tatggcgata caattttgac aaatgcagca aaacaagaac ttgtctcaaa agcaattact
    16441 gttccactaa aatctacagc tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca
    16501 aaaaataaag aaaaatcagt agaacttcta gggctgctta acagtgatcc agaattattg
    16561 aatgggcttg tttgggggat cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat
    16621 aaaatcaaat tgctagatgg ttatcagcca aatacacata tgtctgcttg gaatacagga
    16681 aacaacaaga ttctatatac acaagagtct attacggatg acatgattgc caaacgtgat
    16741 caatcgattg ctgatgcaga aacttcaccg attttaggat tcagcttcaa cactgacagt
    16801 gtcaaaacag aattaagtaa tatttctaat gtcatgaatc aatatttaga tggattgaat
    16861 acagggactg ttgatccaga tgaaacatta ccgaaattga aagacgcttt ggacaaggct
    16921 ggctatgata aggtattgaa agaaatgcaa aaacaatatg atgagttccg acaagaataa
    16981 taatgaataa agacaaaggg gttgtgacaa aagacgtgtc acaacctctt gttccgaata
    17041 aacggtgttc aaaagccagt ttctcggtat taagctaaat atatgtaaaa atgtggaaag
    17101 ccatattcac aaaatttttc ttactactcg aaactaagcg gctatttcac aaactcttgg
    17161 taaacggtgt tcaatcagct aaccctcggt attcgctcac aaaacgacaa aatatgagga
    17221 actatttttc cgtttgtttc tctaataatc aggtcaaggt gctgattgcc caaccttttt
    17281 ttgtataatg agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt
    17341 tggaagagtt atttatccgg atctgggtca ttataaaact aaccttgtat ttctggatat
    17401 atacatttgc cggcgggatc atctttggat taggtgcggc atggaaaaca gtcaatgaac
    17461 tattttattt gtacggattc gagtataaag aaatcacaat aaaaagaggc tggaatatat
    17521 ataaaagaaa ttttttaaga ggcaatctgc tatttagttt atttttatct ggtactgctt
    17581 tattgagtta caatttatat ttatctgttc aaatcaaagg gctcattttt ttagctattg
    17641 attttatttt actttttgcc ttgctctgcc tatttgctac ttatcaatac agtatgattt
    17701 tagatagtga atatacgatc tctgttccta atctgctaaa gctaagtttt atttcggttt
    17761 tttccagctt ttcgtctttt ctaaaaataa ttattggtag cggtataatt ttaggcgtga
    17821 cttggcaatt caaaggattg atcctgtttg gtgtcattgg tcttttgacg gtttggaatg
    17881 gtacgatgac aacacattgg cgtgaagaat tagataaaca gttggaaagt tatgaataag
    17941 tttcggattc tattttcaaa aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt
    18001 atcatgatcg gtgtaacgat gactgctacc gttattttta gtatccatac ttgggaacaa
    18061 aatcaaaaac aggcagaaaa catgacaagt gatgccgttc aaagcacaag cagaatgctg
    18121 aatgataaaa caaccttgag taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag
    18181 atcgaaaatg tgacgaccta cttaacaaaa cccatagatc agtatctgat gtatgtatac
    18241 gaacagcaaa acagtaccga tgaattagtt tctttcccaa atcaaatcaa agatctctac
    18301 gcaaattatg aagaactaag tgcgatatat atcgtgttga atcaactacc ggagtattat
    18361 gaatcttcta gagaaaacaa aggtggggaa ataaaaaagg ggacaccaaa attactagac
    18421 gccttttata tcaagttgcc tatcacacaa ggaggagctg agtcaggaag tatttttatt
    18481 ggttttcaaa agaaagaact agatatgatt ttagaaaatc taacgtcatt caatggactc
    18541 tctttataca tgatttctgg aacgacaaat cgactataca cattccatga taataaaata
    18601 gctaaagaaa cgtttgaaag acaagaaatg atcatcacag agagtctgaa gaaaaattca
    18661 caattgccga ttgacgatct ggaaaaaaac aattttgttc aacaccaaga actgtctgat
    18721 gattatcgta tattagctgt actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat
    18781 cttgccccgc tgatcattgg tatcattatt ttagacaata tactgcttat ctttctttat
    18841 aaaacattca aacgatattc acagcaagta tctatcgtca tgcaggccat ggatcaaaca
    18901 gcagcaggga atttagagac gcggatcgac ataacaaaca cggaatatga actgaaggaa
    18961 ctttcaatcg gtatcaacga aatgttggat agcatcaatc agtttgtaga agacatctac
    19021 aaattagaaa tcaaacagca agacgcacat atgcgggcgc ttcaagcaca gatcaatcca
    19081 cactttttat ataatacatt agaatatatc cgaatgtatg caatcagcga aggaagtgaa
    19141 gaattagcag atgttgtgta tgcgttttcc gctttgcttc gaaacaatac gaatcaagag
    19201 aaaacgatca ctttaaaaga agaactggac ttttgcgaaa aatatgtcta tttgtatcaa
    19261 atgcgttatc ctaatcgagt agcctatcat tttatgatcg atcctgattt ggaaaagatc
    19321 gaagtaccta aatttgtgat ccagccatta gttgaaaatt attttaaaca cggtattgat
    19381 tttactcgat tcgataatgc gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg
    19441 atcatcatca aagataatgg aaaagggatg actgaaaaac ggctaaaaca gattgaagaa
    19501 aaactgtctc atcctaaagt ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa
    19561 cgtttacgtg ccagcttcgg cagcagttat tatatgtcgc ttgagaataa tgagacaggt
    19621 ggactgaccg tttctattac attcaaggag ggatgagcgt gtatcaagtg ttattggtag
    19681 atgatgaata catgattttg aatgggttaa aaaagataat tgattggcaa agtttaggct
    19741 ttcaaatagt agcaacagca gagaatgcca aagaaggact agctgtactt gaacagcgac
    19801 agattgattt agtagtaacg gatgtgacaa tgccagaaat aaatggactg gaatttattg
    19861 aagcggctca aaaagaacgt cataattttg aatttatgat tttatcagga taccaagaat
    19921 tcgattattt gaaaggaggt atgcaattag gcgccgtgaa ttatttgatg aaacccgtga
    19981 ataagttcga attggttgaa agcctcaaga aaatcaaaac acggttagat cagcaaaatg
    20041 aacaaaaaaa tcaacaagaa atctatcaag aactgttatt cagtcaatgg ttgaatgaag
    20101 aattggatga aataagtgaa gaagaaataa taggacaaat aagtgagaag cagcatagga
    20161 tagtattgat tcaactggca agaactcatg atactcttat caatagatgg ttgcaagaac
    20221 ggcaacaaat cttttactat caacgaaatt acggagaccg tgtattgatc acgttgttgt
    20281 tggaggcaga tgaagttgat ggcttctgtt gttttgttca agaaaacttt cctgatcagg
    20341 aatggctgat cagtatcggg gaagagacag attctttgga caaaatccct gaaacattcc
    20401 aacaagcgaa agacaatcta cagttgcatc aattttatgg agataaagac gagcatgttt
    20461 tttacgcagt acaagcagaa acaaaagatc aaaccatcga tttttcaagt ttcagacgcg
    20521 tgctgcaaaa taaacaatta gaagtcgctc aaaaaatgat cactgatttt tttgaacagt
    20581 ttcagttagc agccatgatg ccggaagata tacgatattc cagtttttta ctgtttatgg
    20641 agattcaacg ggaattgatt gatctggaag atgaggaata tttacaagga atcgaaaaaa
    20701 tccatcaggc aaaaacggtg caagaacttc agcagctact tctgtcattc atcaaaaagc
    20761 atcagagaca gaaaaagtac agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc
    20821 attatcaaga accacttaca ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt
    20881 atctaggaca gctgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt
    20941 tacgtatgga aaaagcgaaa caactgcttt tgcacagtaa tcaaaacatc aatgagatag
    21001 caagtgagat tggctacaac aacaccacgt atttctctaa attattcaag aaaatcgtcg
    21061 gcagatcccc taaagagtac cgagaaaaca gtggagaatc ataaaagaaa aagccaatgt
    21121 aatgaaataa cttataaaaa attgtctaga aatctaattt tgggctgttc ttattacttg
    21181 aggcaaggca gctttttcat gacctctcga taaacggagt tcaaaagcca gtttctcggt
    21241 attaagccaa atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttaatact
    21301 cgaaactaaa cagctttttc acgacctctc cactaaacgg tgttcaatca gctgaccctc
    21361 ggtattagct caaaaaatgg caaaatatga ggaactattt tgccatttgt ctctctaata
    21421 ctcaggtcag aacgctgatt tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct
    21481 gcggtaataa gaccaaccaa acgaaaaata tgaggatcta tttttgcttg gctgttctta
    21541 ttacttgatg caggacagct ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa
    21601 aggcaaatct tttgcttgat atttcttagg gtgagggatg atccctaatt ggtaagagat
    21661 cgtgccgcct ttcagcaaat cttgatgggt aaaatataat ttcgtatatt ctttgtccat
    21721 ccgtttgatt ttatggataa attgttgctg cggccgatta ggcgttgctt caatcgtcaa
    21781 ttcctttcca tttgacaaaa gcaaaacagc cttgtcaatt aatggcatac cgataacata
    21841 ttcaccggtt ccaggcgtga caggataaaa tcccagacta ttgaagatat accaagcggc
    21901 catacttcca ttgtcctcat ctccagggta acccattgga gaatcgtcga aggtctgagt
    21961 catcaactgt ttaagcagtg gctgtgccat ttcaggttgt cctatataac tgaacaagta
    22021 aggatagtga aaacttggct ggtttgaaat agctaattgt ccaaattcta tcgcggccat
    22081 ctcactcatt tcgtggatct caaaaccata tcctcctggg tcaaacgttg ggggctgatt
    22141 gcacagctca atgagttttt ctttatatct ctcactgcca ccatattttc cgatcagtcc
    22201 tgaaaaatcg tgataaacag caaagctgct ttgccaagca cttccttctg cataatcttg
    22261 tccccaacga ttagctgaaa atggttctct gaacttgcca tctttatctt ttgaacgcat
    22321 aaatcctgac tcatagtcaa agatattttg atagttcaat gattgctgct gatatttctt
    22381 cgtcatttcc tcttcttcta gtaccttggc aacttggcta atacaaaaat cgctataagc
    22441 atagtctaat gtgtgattga ctgattcgtg gtagtgatta ggaacatagc catatgtcaa
    22501 gtaatcttct gttccttggc gtccataatt tggatgtttg ctttgaaccg tagcaccttt
    22561 ttccattgcc gttagaaatt caggcatcaa gtctcgtcgg atatctttta cagcagcatc
    22621 tgcaatgacc gcatcgatca atgtcccagg cataagtcca cgttcatcag gagacagcca
    22681 ttttggcaaa tagccacttt ctctatagct gttcaaaaaa ccttccagca tttcttcgta
    22741 tttttctaca gcaatcaaag aataaagagg ataaaccgtt ttatacgtat cccaaaaccc
    22801 attatttgta tagagaacac caggttttat agatcgacta gtcgtatcat agtggatttt
    22861 ttgcccacta ggatcaattt cataaaatgt ttgtggaaat aaaaacaccc gatataaatt
    22921 atgataaaac gtccgcaatt gttgttgatc atggtgtgtg agctgtatac gatcaaaata
    22981 ggactgccat tgttcttgac tgtgagcaag gtaagtctct ttgctttggt tttgttctag
    23041 ttccaggtta taagctgctt gttccgagct gataaaagat gtcccaaatc ggatagtttg
    23101 tttttttgtc tgttcaaaat acaacgggat cgagtcgcta tttttgttgg aaacagctaa
    23161 ctcatcaatt gttaaaggat attcaaatct caatataaaa taaaaagaaa aatcaggatc
    23221 ttctgaacca gcaaaatgtg tgatttttcc ttccactgtg taaggatcta taacaaagag
    23281 ctgatggcgt cccggaaagg ataaaagtaa accgctgtca tttttgctgt aatcaataga
    23341 taagattccg ccatacatgc taggtatcaa tgtagaacag atgccataac gtaattgaca
    23401 gatcgacaga tgagtcgggc aaaatatact ttcttctggg cggtaagaac tttgagcatg
    23461 gaaaagggtg ttctcctgta gttttccatt gatcggcgta aacagcatat gactgaaatc
    23521 acccatccaa ggacttggtt gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg
    23581 atgaaaccac cagctgccgt tttgatccgt agtttgcggt gcaaaataat tcatcccaaa
    23641 aggaacgcca gtataaggct ggcagttccc attagagaaa tttggctgat tcgctgttgc
    23701 atgtctagta tctatttgac cgatattcat tttatttctc ctttgttgat tgtcttttga
    23761 tgaatgaaac tggaaattta ctatcggatg gtcgatcttt tgtctctaac caagtgacca
    23821 aagcatctcc cgcaagctta cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg
    23881 ggtcaacaag agctgctgct tggatatcat caaatccgat aattgaaaaa tcttcaggga
    23941 tatgataacc atgctgtctc aagagattca tggtctggat agcgacaaca tcgttttcac
    24001 aaacaaaagc agttacttca tacttagtga tataagaaag caattcatct aatgtcgcgc
    24061 ttttgtcatc cagtgaagtg tggaaagtca aaccagcttt gtccaatgct tcaaggtaac
    24121 caaggtaacg ctgtcgaacg gattgtggat gagtctgcgt accaaacaaa taagcgattt
    24181 ttgaatgtcc ctgttcgatc aaataagagg cagcaagcgc accgccttgg aaattatcgg
    24241 aaagaacggt aggaaaaggt aactcataga tttttttatc taatatgacg acagggaatt
    24301 ctttcaatga aagctcaaaa agtaagtcaa gatgttgttc tgtatcaaga gcataataaa
    24361 tcaatccgtc aaattcattg attagctctt ttgctgtttt attttgtaga aagtcaagcg
    24421 tcgtcatcat gacatcaatc tgtttatctt gcatcacagg gttcaatccc ttgctaaaat
    24481 caccaacaga caaatcagcg acatatggaa gaagaaacag aatccgtcgt gtttttgtag
    24541 gagaagcaag aatctgttcc ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc
    24601 gataaataag tcctaattgc tctaattcag tcaatgcacg tttggaagtg attctgctta
    24661 ctttataggt acgtgaaagt tctttttcag ttggtagttg gctgtctaca ggtaattcac
    24721 cagttaaaat agccgtttgc aaatcattaa atattgtctg gtacaatggt tgattcatat
    24781 aaacacctct taatgatata tcatattcaa ataataccat aatggatgaa aaagtaaata
    24841 aaaagatatt ttttgaaagc gtagacataa ttggctgtat gaagtataat ctttaaagat
    24901 atatcaaata gaaagaaggt tttattagtg gcttataaag atataccaaa atcggtgcag
    24961 aaatttatgg aagatatcac acaaaaatgt ggggatatcc atgcggactg ggcaaagaat
    25021 tttacagcag catttgccaa tactctgttg acaacagtca aaaagcaaaa tgacggaacc
    25081 acctttttat tgacgggaga cattccagcg atgtggttaa gagactctac tgcacaagtt
    25141 cgtccttatt tagtgattgc aaaagaagat gaggaattag ctgacatgat cagcgggtta
    25201 gtcaaaaagc aattttttta tatcaatatc gatccatatg cgaatgcctt caacgaagaa
    25261 gctaacggtg ctggccacca aacagatcat acaaaaatga acgattggat ctgggaaaga
    25321 aaatatgaaa ttgattcttt atgctaccca atacagttgg cctatcttct ttacaaaaat
    25381 acaggaaaaa ctgatcaatt caatcaatct tttattgaag gtatcaaaaa aatcttggac
    25441 gtgttcaaaa cagagcaaga tcatgaacac tctccttata catttgtacg agacacgaca
    25501 agaaaagaag atacgctgat caatgacggg cgaggtgcaa aaacagcttt tacaggaatg
    25561 acatggtctg gtttccgtcc tagcgatgat acttgccgtt atggatatct ggtaccatcc
    25621 aatatgtttg cggtggtcgt gttaggttat gtggaagaaa tttttcaaac gatactcgat
    25681 gataaagaaa ttgtacagca aacacagaaa ttgaagaaag agatccaaga ggggattcag
    25741 aatcacggac gaacggttaa tcaaaaaggg caaacgattt atgcatatga agtagatgga
    25801 atgggaaacg ccacagtcat ggatgacagt aatgtgccca atctgatcgc cgcaccttac
    25861 ttgggatatt gttctgaaca agaggaagat tatctgcaaa caagaagaac attgttgagt
    25921 aaagaaaatc catatttcta tgaaggaaag tacgcaaaag gaattggatc ctcccatacg
    25981 ccagaaaaat acgtttggcc gattgcatta gctatggaag gaatgacaac gtcagacaaa
    26041 tctgaaaaag agcggatatt gaatttactt gtagcaactg acgcaggaac acaccttatg
    26101 cacgaaggat tcgatgtgaa caatcctgaa aattatacga gagaatggtt ttcttgggca
    26161 aatatgatgt tttgcgaatt agtcatggat tactttgata ttagagtaga aaagtaggag
    26221 gtatcgtaaa tgaaaaagaa agtatatatc atttctcata gccactggga tcgtgaatgg
    26281 tacatggctt acgaacagca tcacatgcga ctgattgaat tgatagacga tctactagag
    26341 ctatttgaag ttgatccgtc cttcaacagt tttcatttgg atgggcaaac gattatttta
    26401 gatgactatc ttcaagtaag gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa
    26461 ggaaaattga gaattggacc attttatatc ttgcaagatg attttcttat cagctcggaa
    26521 tcgaacgtga gaaatatgct gattggcatg gaagaaagcc gtaaatgggg aacccctgtc
    26581 atgttaggct attttcccga tacatttgga aatatgggac agacgcctca attaatgaaa
    26641 caagctggta tttcagcagc agcgtttggc cgtggtgtaa aaccaattgg ctttgataat
    26701 caagttttgg aagcagaaaa ctattcttcc caatactcag agatgtggtg gaaaggacct
    26761 gatcaaacag caatctttgg tctgctattt gccaattggt atagcaacgg gaatgagatt
    26821 ccagtagaaa aagaagcagc tcttgccttt tggaagcaaa aattagcgga tgcagaacaa
    26881 tatgcctcta cgaaccactt attgatgatg aatggtgttg atcatcaacc ggtacaaaaa
    26941 gatatatcaa aagcaatcca tctcgcaaat gaactgtttc cagattacga gtttattcat
    27001 tctaatttta ctgactatct ggaagcagtt caaaaagatg tgccagaaga cttaggctca
    27061 gtagaaggag aattgacaag tcaagaaacg gatggctggt atactttagc caatacagca
    27121 tctgctcggg tttacttaaa acaatggaat accaaggtcc agcgacagct agaaaatatt
    27181 acagaaccac ttgcgacgat ggcttatgaa gttagtggaa actatcccca tgatcaatta
    27241 gattatgctt ggaagacatt aatgcaaaat catcctcacg acagtatttg cggttgttca
    27301 gtcgattctg tccatcggga aatgatacct cgttttgaaa aagcagatga agttggaaag
    27361 tatcttgcac aagattcctt agaacagctg accgcagcaa tcgatacgac cggattccca
    27421 aaagacagct ttccatttgt cattgtaaat actgcaggaa tggataaaac tggtgaagca
    27481 gaaataacca tcgaattaga aagaaaaaga ttcgctgaag gaatacctga acaactatat
    27541 caagagttag aaaatttgcc taaacgtaag tatcatgtag aaacaaaaag tggggccact
    27601 ataccagcta ttttatctga agaaactgtc caatttggtt atgatttgcc gaaagaccgt
    27661 ttccgtgtgc catatatggc aagaatgatc aaagtgactt taccattaga aaacatgcca
    27721 gcattttctt gggaaacatt cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa
    27781 acaatgattc atcagagtgg acgaatcatc gaaaacggcc ctctacattt gacgattgaa
    27841 aaaaatggta ccattacaat ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat
    27901 atttttgaag atatcggcga tataggaaat gagtatatct ttaaacaacc tttctgtgat
    27961 cagccaatcc tatccagtaa caaagaaaac tctgaagtaa aagtattagt agatacaccg
    28021 gaaatagcaa aaataagtat tctacaggaa atggaaatcc ctgtttctgc agacgagcgt
    28081 ttagaaaaag aacagcagat ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa
    28141 gaaaaacgaa tccttcaaat aaaaacaatc atgacaatca gaaaagatag taaaaaaatc
    28201 gactttgaaa cgactctcga caatcagatg aaagatcatc gcttacgtgt cttgtttcct
    28261 acaaagttac atgtggaaca tcacgaagca gatagtattt ttgaagtggt gaagcgccca
    28321 aaccatgtgt ctaaaagttg ggaaaacccc acgaatccgc aacatcagca agcatttgtc
    28381 aatattcatg atgaagagta cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa
    28441 gtaacagaag acggacaaat cgcagtgacg ctacttcgca gtgtgggaga actaggcgat
    28501 tggggatatt ttccaacacc agaagcacag tgtctaggtg agcatcggtt caattacagc
    28561 attgaactac atggaccaga agaaaaattc tctacttatt tgcatgcata cgcagcacag
    28621 attccttttt ccactcaaca aatcaaacat catgaaggaa cattgatttc aaaacaacaa
    28681 tatttaacga taaaaagtga aacgtttgca attacagctt tgaaacgcag caagtttagt
    28741 gataaagtag tagtaagagg tttcaatatg tctagtcatt tagaaaaatt ggaaataact
    28801 aaagacaatg gaaaaactgt catattgaat ctgcttgaag aaccaacgaa gcaagcggtt
    28861 gtcccaataa tccaacctta tgagattcgg acaatcggat tcgaggagga aaacgaatgt
    28921 atggcggaat agaagcaggc ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa
    28981 ttttaga
//
LOCUS       DASJRQ010000025        28453 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJRQ010000025 DASJRQ010000000
VERSION     DASJRQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.025"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(116..1165)
                     /gene="recA"
                     /locus_tag="U4A80_001023"
     CDS             complement(116..1165)
                     /gene="recA"
                     /locus_tag="U4A80_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1974656.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1274..2515)
                     /locus_tag="U4A80_001024"
     CDS             complement(1274..2515)
                     /locus_tag="U4A80_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1974657.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2755..3333)
                     /gene="pgsA"
                     /locus_tag="U4A80_001025"
     CDS             complement(2755..3333)
                     /gene="pgsA"
                     /locus_tag="U4A80_001025"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1974658.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3542..4465)
                     /locus_tag="U4A80_001026"
     CDS             complement(3542..4465)
                     /locus_tag="U4A80_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974659.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5204..6490)
                     /locus_tag="U4A80_001027"
     CDS             complement(5204..6490)
                     /locus_tag="U4A80_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1974660.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6483..7751)
                     /locus_tag="U4A80_001028"
     CDS             complement(6483..7751)
                     /locus_tag="U4A80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1974661.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8003..8464
                     /gene="mscL"
                     /locus_tag="U4A80_001029"
     CDS             8003..8464
                     /gene="mscL"
                     /locus_tag="U4A80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1974662.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8523..8969)
                     /locus_tag="U4A80_001030"
     CDS             complement(8523..8969)
                     /locus_tag="U4A80_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974663.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9062..9307)
                     /locus_tag="U4A80_001031"
     CDS             complement(9062..9307)
                     /locus_tag="U4A80_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1974664.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9422..10000)
                     /locus_tag="U4A80_001032"
     CDS             complement(9422..10000)
                     /locus_tag="U4A80_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1974665.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10077..10607)
                     /locus_tag="U4A80_001033"
     CDS             complement(10077..10607)
                     /locus_tag="U4A80_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1974666.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10611..11471)
                     /gene="rlmB"
                     /locus_tag="U4A80_001034"
     CDS             complement(10611..11471)
                     /gene="rlmB"
                     /locus_tag="U4A80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1974667.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11464..11859)
                     /locus_tag="U4A80_001035"
     CDS             complement(11464..11859)
                     /locus_tag="U4A80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1974668.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11856..13268)
                     /locus_tag="U4A80_001036"
     CDS             complement(11856..13268)
                     /locus_tag="U4A80_001036"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1974669.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13265..13801)
                     /gene="cysE"
                     /locus_tag="U4A80_001037"
     CDS             complement(13265..13801)
                     /gene="cysE"
                     /locus_tag="U4A80_001037"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1974670.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13887..14126)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14123..15583)
                     /locus_tag="U4A80_001038"
     CDS             complement(14123..15583)
                     /locus_tag="U4A80_001038"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1974671.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15753..16910)
                     /locus_tag="U4A80_001039"
     CDS             complement(15753..16910)
                     /locus_tag="U4A80_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1974672.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16944..18314)
                     /gene="radA"
                     /locus_tag="U4A80_001040"
     CDS             complement(16944..18314)
                     /gene="radA"
                     /locus_tag="U4A80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1974673.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18377..18904)
                     /locus_tag="U4A80_001041"
     CDS             complement(18377..18904)
                     /locus_tag="U4A80_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1974674.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19134..19940
                     /gene="proB"
                     /locus_tag="U4A80_001042"
     CDS             19134..19940
                     /gene="proB"
                     /locus_tag="U4A80_001042"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1974675.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19937..21184
                     /locus_tag="U4A80_001043"
     CDS             19937..21184
                     /locus_tag="U4A80_001043"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1974676.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21260..21604
                     /locus_tag="U4A80_001044"
     CDS             21260..21604
                     /locus_tag="U4A80_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974677.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21721..22047)
                     /locus_tag="U4A80_001045"
     CDS             complement(21721..22047)
                     /locus_tag="U4A80_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974678.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22662..23864)
                     /locus_tag="U4A80_001046"
     CDS             complement(22662..23864)
                     /locus_tag="U4A80_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1974679.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23981..24496
                     /locus_tag="U4A80_001047"
     CDS             23981..24496
                     /locus_tag="U4A80_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1974680.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24695..25141)
                     /locus_tag="U4A80_001048"
     CDS             complement(24695..25141)
                     /locus_tag="U4A80_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1974681.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25188..26666)
                     /gene="msr(C)"
                     /locus_tag="U4A80_001049"
     CDS             complement(25188..26666)
                     /gene="msr(C)"
                     /locus_tag="U4A80_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1974682.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27020..27649)
                     /locus_tag="U4A80_001050"
     CDS             complement(27020..27649)
                     /locus_tag="U4A80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1974683.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27633..28097)
                     /locus_tag="U4A80_001051"
     CDS             complement(27633..28097)
                     /locus_tag="U4A80_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1974684.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT         7979 a         6144 c         5086 g         9244 t
ORIGIN      
        1 tttatgtacc cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg
       61 caggtcaagg agagtaccta ctcttcagac cagccgtttt tgtttatcat aatttttatt
      121 cttctactaa aggcaattct tcttgtcctt tttcatcttc tgggacagcg acttcttcac
      181 caataccata agctgctcta actagagcag aaacttctgc catcatttca ggatgattag
      241 ccatatagat tttagcgttt tcccgtcctt gaccaatccg atcttcttta taagaatacc
      301 aagcaccgct tttatcaaca atgtcttttt ctactgccat gtctagaagt tctccttctt
      361 gtgagatccc taaaccatac atgacatcta cttcagcgat tttgaaagga ggtgctactt
      421 tgttttttac gactttgatc tttgtacggt taccaacaat atctgtacct tgtttcaatt
      481 gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag
      541 gagtgatttc tggattcgca aacatcacgc ctactttttc acggatttgg ttgatgaaaa
      601 tagcgattgt ttttgttttg ttgatcgaac cagagagttt acgcaatgct tgagacatca
      661 aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa
      721 ctaaagcagc aactgaatcg actactacga tatctacagc cccacttgat actaaagcat
      781 cagcgatctc tagaccttgt tctcctgtgt caggctgtga aagaagtagt tcatcgatat
      841 tcacacctaa tttttgtgca tattgcggat ctaacgcatg ctcagcatca atgaaagcgg
      901 ccgttccgcc atttttttgt acttctgcaa tagcgtgtag tgcaactgtt gttttacctg
      961 aactttcagg tccatatact tcgatgatac gtccacgggg gtaaccgccg acacctaaag
     1021 cgacatctaa cgcaagagag ccacttggaa ttgtagagat ttgttggtcg attttttcgc
     1081 ccaatttcat aatagagcct ttaccatagc tcttttcaat ttttttcagt gcagcatcta
     1141 aggctgcttt acgatcatct gccaatacga taatcctcct tatatttaca aatcagagca
     1201 acatttcatt atccttatca tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa
     1261 atattcgcat ttatcatttt cttaaaagct gttttctcaa aagatcaaat ccttgcataa
     1321 ccgcattata tctgatagca gcacgatctc tggcaaagtg atatttttcc gaaaccacgc
     1381 cgtctttaga tgaaatagcg atccatactg ttcctgcaga ttgaccttct aacgtgtccg
     1441 gtccagccac tccggtgaaa gctacactgt aatctgttct tgctatctca cgtgcttgaa
     1501 tggccatctg ctccacacat tctttgctga ctgttccata ttctgctaat agctccggat
     1561 caatctccag aaaaccagct tttgtttgga gcgagtaggt aacgaatccg cctggaaata
     1621 cttcagatac accagcaatg tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt
     1681 ccgctgctgt tacagtctgc ttgttttctt tcagtagttc cactacgact tttgctagcg
     1741 aattatcatc gccgtatcca taaaaatatt cccctactcg ttcttggatt ttttcttcta
     1801 atgctaaaag agcttgattc cctgcctgaa catcatttgt tttcactgtc agtcgtaatg
     1861 tcacttcatt tggtttggca tatggtgcga tcgttggatt gatctgtgtc tcaatcagat
     1921 cttttagctc agtgaccagt cgcgattcac caatcccata aaagcgcaaa actttagaaa
     1981 ttagtttttc ttcagaagga aaacgttcct ctagtaaagg acgtacttgt tccacaaaca
     2041 ttggtttcaa ctcattaggc ggaccgggaa gaaggatata cgcatgtgta tctgtttgat
     2101 aaaaagtccc taaagcaagc cctgtacgat ttggtagagg aaccccgcct tcgataatct
     2161 gggactgttg cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata
     2221 attttttgta tccttccgta ttttgtatga gagacttacc gaggtgagcc gcagttgctt
     2281 cttttgtcag gtcatcttct gtcggtccta atccgccaca taagagtatg agttcacttc
     2341 ttgtttcagc tagagcaatc aactcttcta aacgctgctg attatctcct acaactgttt
     2401 gataataaac ttcgatacct agatcagcta gctgttctga caagaaagtg gcattcgtat
     2461 tcacgacttg tcctagtaaa agttctgttc cgacagctat tatttctgct ttcattactt
     2521 cccgctccct tatatagtat acgctgtatt tttatatttt tttatttaaa aagagggcaa
     2581 ggagctgtga caaaaatctt gtcacagctc cttgttccaa ataaacagtg ttctcctgcc
     2641 aaaacctcgg tcataagtca caatatacat aaaacatgaa aagctgtttt ttgaaattat
     2701 cccttgtgac tcagagttag acggcaggct cacaacctct ccactaaacg atacttacat
     2761 tgatcctttg aacacaccgc tgtttttgat gaagtaatcc acacctgaat agatagtaaa
     2821 gatcagacaa gcataaagca tgatttgatc tactggaata ccgatcaggc tgaatgggat
     2881 attattgata aacagcaaga taatagcaac catttgtgta gccgtcttga cttttcccgg
     2941 ccaagctgca gccatgactt ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc
     3001 taattcacga cagacaatga tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa
     3061 catgataaaa gcagtcataa ctagcatttt atctgccaac gggtccgcaa acttgccaaa
     3121 attagtgaca aggcctcgcg cacgggcaat ttttccatct aaccagtctg tgatactagc
     3181 taaagcaaaa ataactgcac ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt
     3241 aaattgtccc caatctaaag gtaaacctac aaccaaaatg aaaatcggga tcatacaaat
     3301 tctaagtaca gttaatttat ttggtaaatt cacactatcg actcctttct tccctattct
     3361 acgtgactat cagcgaagtt tccaccgata ttatttactt ttccaaatga atttattcaa
     3421 aggagtattt ttattgaaat tgatcttttt gaattaacga agaaggaccg cgacacagat
     3481 ttgtcacgat ccttcttttt tatacctaca agtaaacaga tgcgtaaact aatttatacg
     3541 atcattctgc gtattgaaga gtaaggttca cattttttgg actctgattg ttttcccctg
     3601 gattaactgg tacggattct ccattcgctt tgatcgttgc attgcttgct gcaccaatcg
     3661 tgatggtcgc atttgttgct gtttccggta acacggtact ctgtgtttca tttgctgcca
     3721 gtgtcccttg ataaacatag gcattgtcta ctagcacacc taaccataca cgattgacag
     3781 cagtaaattc aagggttaca ggcttggtag cgtctgtgat atttactgcg atggcactgc
     3841 cagaatcttc tcctgtagct attgccattt tcttttcttc acttgatttt gttgttgatt
     3901 ctactgtaga agactctttg gtctgcgtgc tttgctctga ggaagaggac tcctcgactg
     3961 atccttctac tttaagcgaa gaagctgtgt ctccgattat tggatcagct tgacgatctt
     4021 gccaagtcat atagcccacg acaacgacaa tcgccaaagc aaccaatcct aataaaatga
     4081 caggaagact tgtccaaaag cgacttggat gcgattcttc ctgatgcatt gcttttctag
     4141 aagtacttac tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat ttgcttttcc
     4201 catcaaaaac atcgactaaa tggtctccat cttctcctac agcttcagcg tattgccgaa
     4261 taaatgcccg cacgtaaaat ttaccaggca attggtcaaa atcatcattg tcgattgctt
     4321 ccaaataacg tttttgaatt tttgtaatct gctgtaattc atctaaagat atttttttat
     4381 tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa agccactcgt tcatcttctc
     4441 cagtctgttt gattctccga agcatctgct gttgcaacga aagacgttat gtttgggtgc
     4501 tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa acattgttct cctgccaact
     4561 cctcggtcac aagtcacaat atccaataaa tataagaagc tgttttttga acttgttcct
     4621 tgtgactcgg agttaaacgg caggctcacg acctctttct taaacggtgt tcaatcagct
     4681 gttcctcggt attagctcaa aaaccggcaa aatataagga gatatttttc cgtatgtttc
     4741 tctaatactc aggtcaaaac gctgatttca caacctcttc cttaaacggt gttcaaaagc
     4801 ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt aagaagctgt ttttacttgg
     4861 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttggtaca cggtgttcca
     4921 gaagtagctt cttcggcaat aagccgaaat atcacaaaaa tttgagaagc aattttcgta
     4981 aattccttct tatttcttga agctgaccac ttctgtcaca acctctttga ttacttcgtt
     5041 tgattttatt ttaatataag ttgattaaaa gagagcatga tttgttttat tttctcacta
     5101 atttttccaa cacaactaag aataccataa aagctgattt tggaggaggt tcctactaca
     5161 agtctaaaac gaaatttaag acttttcctc ataaaatact ggtttatttt ggtctcatgt
     5221 aaaactcgct gaaggcctct tcacttataa atgcttcccc agctgccagt acgtctttca
     5281 tttggatcga atcgatgatt tcaggaagat caaataaagt tcgatcgcca aataaacttt
     5341 gcgtaaattg attggcaata tattcgatcg aatttaatga ttggaaatat tgtccaagca
     5401 ttttcttctt cagcaaatcg agatttgttt ctgacacttc cggatcatca gcgaatccaa
     5461 gaatgatttc tttgactttt tctgctgctt tctctggttc gtccgtatct ccgctgaagt
     5521 ccgcaaagtg aaattcacga tccaagctga attcaaaacc aaagctgtca tcgataattc
     5581 cttgattgta catcgctaga tagtttcgag aagtattgcc taaaatcatt tggaacaaca
     5641 gattgattgc tgttttatag cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc
     5701 ctttaatacc aagtacaaat ttatctcgtg taatggctgc ttccaatgca ctttgtttga
     5761 tgatttcttt tgtgttttca gggaaataac gtacaatttc ttgctttgga gggaaatttt
     5821 ttgcttcttg gttttcccga ataagtttca tcaatttctc aggctccatc ttaccaacta
     5881 caaataaaac catattgctt ggctgataaa acgtccgata gcacgtatac aagtcttgag
     5941 ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag
     6001 gatacaagtt atttagtatg ccaaaaaaca tccgccagtt agggtcgtct tcatacattt
     6061 ggatttcctg accgatgatt cctttttcct tgtttacagt ttcttctgtg aagtaaggtg
     6121 cttgtacaaa atcgatcaga gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca
     6181 gatagctggt tttcgtaaaa cttgtgaaag cattggcgga agctccttgt ttcccaaata
     6241 attgaaagac atcaccatct tctttttcaa acaacttatg ttctaaaaaa tgggcaattc
     6301 catctggaac tttcaccttt tctttttcac catagggaat aaattcgtta tcgatcgaac
     6361 cataattcgt actgaacaaa ccatatgtct tgtgatagtc atttttaggc aacaaataca
     6421 cggtcaaacc gtttggcagt acttcatgat aaagtgtttc attgatttgc tcatattctg
     6481 ttttattcat ttgcatttcc tcctgctaaa aagaagattg cctgcaattc gatttgctca
     6541 gctacttgtt gtatctcttt gatcgttacg ctctccatac gtttcaacca ttcttcatca
     6601 gataattttg tttccggcag ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga
     6661 gagtctagag aaagcagata ttggtttctc agcattgctt ttgtctgaga cagttccaac
     6721 tctgttattt caccatttcg caaactttct aattgctcgt gaatcagatg aagtacttga
     6781 tttcggttct tttcatcgat accggtttgg acactcatga atcctcggaa cgtatccacg
     6841 ctactagagg cataataagc tagactttct ttctctctga cattcatgaa aagtttagag
     6901 tgaggaaaac cgccaaataa accgttaaag accattagcg caaaacgttc tggttcatcg
     6961 taatacacat ttgtctgata agctaggttc agctttgctt gaacgattgg ctcacgtact
     7021 tgtcgttcct tcacgatatt cacttgaggt tgggtataaa acagatctgg ataaactgtt
     7081 tcccgcgact caaatggtaa ttgcccaact gcttcagctg ctttgtccgg atcaacatcg
     7141 ccgacaacaa aaatgtcgat ttgatcattt gccatcattt cttgatagta ggacgctagc
     7201 gaactagcag tgatcttgtc caaatcttcg actacgccca accctgggat cttttgttcc
     7261 gggctgtttt caaaataaag ttcttgcaag gcataggaag caaaagtttg tttatcttcc
     7321 ttcaaacttt ccaaatatag acgcagattg ttcttttcca agtcaaacgt caactgatca
     7381 aactgatgat ttttaatatt tggataaaat aaaacctctt ttaaaaaatc taccgcttga
     7441 gaaaaaaggt ttggatcatt gacatattca ccattcacaa aactgatccc tgcgtttagc
     7501 caatgtaaat ttcctttttt tctaactgat aatccaaagc ttgcgccata aagctctgca
     7561 agttgcgcac ttaactttgt ctgatccgaa taattcaaac tatttgtttc caacatgctg
     7621 ctgagaagtg aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa acggataaaa
     7681 atccggatcg ttttgtattg ttttgttggt aagacatgaa gattcacgcc ttctgccaat
     7741 tgatacacca tatttatatg ctccttttca cagttgattg cccgcttact cttttaatgg
     7801 gtcaacctgc cttttttact tattcctctg cttgaaattt ataagttcag gcaattcgcc
     7861 gttttttcta aatgatgtta cactttatag tatagcatag atgtttttta tcaaaagaaa
     7921 cagacggcta aaagcgtata ttgttttttt attttttatt tttcgatata ctaaagacaa
     7981 ataaaagaag ggagggtcat tcatgatcaa agaattcaaa gagttcatca tgcgtggaga
     8041 tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg tttacaagca tcgtaaacca
     8101 agtggtgcaa ggcttgatca caccactaat aggttggttt gtcgcaatga tcacaggtac
     8161 ttctgattta gaaggagcac tttcaattct tgactggtcg ccgacaaaag gcgtcacatt
     8221 tgcgtttggt aacgttatta gtgctatcat tacatttctg atcactggtt ttgtcttatt
     8281 cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag agcaagtgga
     8341 agtggaagaa actcctactg cagaagatta tcttagtgac atacgcgatc ttcttgccaa
     8401 acaagtggca gaagacaata cacatccagt agagcaaaca aatctttccg atacttctaa
     8461 ataataaaga atctaaaaaa aagaaagccg attttcagtg tcggctttct ttttttagat
     8521 tcttaacttg ctacttgttc ctttttcatg tcgatatgtg gaatatcatc ttccaaatac
     8581 acatctgaga ttgccttgaa tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg
     8641 ccgatcacga ttggtttttc tggaaaattt ttctggatca tgttaatcgt ttcttgaaca
     8701 atctttcttc ctaaaccatc tttacggttg tcctgatgaa caaggacacg tccaaaatgg
     8761 atactctccg cttcttcata tactcgcgtg taggcaagga tcttcccatc agtatcttga
     8821 aaaaacgtat gccaagcttc tccatcaatg tcatcaactt cttgataagg acactgttgt
     8881 tccacaacaa aaactgcgat tcgttctttg acgatcgcgt aaaactctgc tgttgacaat
     8941 tcttcaaatt tctttacctg atattccact aagcaaacgc cccctgcttt tttattctta
     9001 gcataccgat tcgcacacaa aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt
     9061 tttaaactgc ttcagttaaa aattcgatct caactgtccg agtaagtaca tctgaatagc
     9121 tgtaagatac tcgttcaaaa gaattttctt caggatccaa atcaacaacg aatactgaag
     9181 gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa
     9241 ccaacatgat tgggctgccg atacgacctt ctaaatcttt cttgattgaa gctaaagttg
     9301 ttggcataca gttcacctct actatcaact ataccacata tttttaaaat tttcaagttt
     9361 accataaaaa aaaaagaaat gcaataaaaa taaataagaa gctcattaag atttttctaa
     9421 attattcctc attataaaat ttcaagtgtt tttttatttt gagttttact ctcccgtaag
     9481 cgctcattac ttgcttgtga tccattgcta aacattcagc catgtctaca aaatcaacgc
     9541 cttgcatata ataatgaaaa attttagctt ctatttttga aaactggatt tcgcaatttt
     9601 cgattgcttc ttttaataaa agatgctgaa ttgctaaagg ttcttgcaat gtgatctctg
     9661 ctaaaaagtc tggtccttct gtctctattt tttggtcgat tgagacttca tctttttgat
     9721 cacgacgctt cagcgcgttc tgcttcctca acagagaata gatttgattg cttataacgc
     9781 ttttataata acttccaaag ctaacacctt tgcttttttc gtaagttctc aatgcctgat
     9841 ataaagcgat ccttgcttct tgttcccaat cctcagaaac ataatttctt aaataatagg
     9901 tgtttttcaa acgtagtaaa agtggaaaat atttttgata cagggcagca aatgcttctt
     9961 cgtcaccatt acgtgcagcg cgcaataact cctcagacat aaaataattc ctccataaat
    10021 tcgtttatgg aggaatctta tcatcaatga ttcatcgaac tgattagaac gatatgttat
    10081 ttattttttc tcattaaatc gtccaatttt tgggacaatt tttttaactg ctcttcattc
    10141 catggagaat tacgtcggaa atcaataaaa tgcatatctt tattttcttg ggctattgtt
    10201 ttttccgttt tttttatcgt cttatataat tcataagcag aagttcgtaa tgctccttgg
    10261 gaaaagatca tccactgttc tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg
    10321 tcattcagct gaccagcgat tctctcgata taactgtcag ccgtttcgtc ttctttcgta
    10381 aaaacaacat ccaactggta tttcttataa ttttgttgga tgcccggaac tagttgggca
    10441 tcgaagacaa cgatgacttc cattccttca tatttcgcat aatttgataa acgatgtaaa
    10501 agagcttctc ttgcatccgc cattttgtcc tgctttttaa gctggacaag ttccggccac
    10561 gaaccaatca tgttgtagcc gtctacaatc aacagctgtt ttttcatggt ctactccccc
    10621 tgacgttggg aaaaagcctg atacataagc aaaccagcag caacactggc atttaagctc
    10681 tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca tttctttttt caatcctgga
    10741 ctcattcctt tgccttcatt accaatgact aaagcaattg atcctttggc attccatttt
    10801 tggaagtttg tgccggtcat atctgtgcca aatacccaaa atcctgtttc tttgagctgt
    10861 tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga catgttcgac tgccccagtg
    10921 gaagtctttg ttacgatagg tgtgattcca actgctcggt gttttggtat gatcacacca
    10981 tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg
    11041 ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt ttgttcgttc aatcagttgg
    11101 tcaagggata aatattcata aggtgtaatc gcaagaacga ctccttgatg cacgccgtga
    11161 tcgctcaaag tttctaattt ttgtttagga acccatttga ctggcacagc ttgttcacgt
    11221 gcgatttctt tcagttcttc tactttttct cctcgtgaat cttcttgaag aaagagttta
    11281 ttccctcgcc cttgttgtag cgcttctact gcggcatggt gcccaaagac gaaattatct
    11341 tctaatgctt cttcattttg atcagaaact gactgatttc gtccagtttg ctttttattt
    11401 gaatatctat tttgtttttt gtcttcaaat ccacgtttcg attgatgttt gtttgttctt
    11461 tctttaccca tttttttcac caactttctg aatacaccag cggatcaatt cttccatccg
    11521 ctcggtttgt tttgtcaaat gcagatagcc catgagcgct tcgaaccctg ttgcgacccg
    11581 atacgtcgta acatcagcat tttttgcgct cgtatggctt ttagcatttc ttcctctgcg
    11641 ataataaagt tcttcttctt ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa
    11701 ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga tgcagcttat tgggttttgt
    11761 ttctccttga tcaatcaggt ggtcacgaac atagatttcg taaatcgcat ctccaacata
    11821 agccaaggct aaaccgttca attgtgtgta atctctcatg cttctcttct ccatcgggtt
    11881 ccttgcggtg tatcttctaa taagatccct ttttctttca acaaatcacg aatttcgtcg
    11941 cttcggacaa aatcacgatt gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc
    12001 tcttcatcta ataactcttc tgctgtaaat aaaataccga agatcgaaag ccattgcgag
    12061 aataaagctt ctccttgtgc caagacagcg gcagaaactt tcggctggtc tagataagtg
    12121 ttcagccact tagcaagctc atagaccact gtgatcccat ttgctgcatt aaaatcatcg
    12181 tccatttctt cgatgaatcg ctgttctaaa ttttctaaca aagcgatttt atccgcatca
    12241 tctgccaaat tctctgaggc agtttcttgg cggaatttca aattgtcaaa cgtattcttc
    12301 agtcgttgta aattggtggc tgcttctttc atcgtcgttt cactgtagcg gatcggacga
    12361 cggtattgtg tggtcgccat gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt
    12421 tcatgtacag tgatgaaatt ccctagagat ttactcattt tttcatcgtc ttcgccaatc
    12481 gttacatagc cattgtgcat ccagtaatta gcgaattttt tccctgtttt cgcttcactt
    12541 tgtgcgattt cattttcatg atgaggaaat tctaaatctt gcccgccacc atggatatcg
    12601 atcgtttctc ctaaatgttt tgttgccata acggaacatt caatatgcca accgggacgt
    12661 cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg
    12721 aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg tacgctgact tgctccgact
    12781 tccagttcat cgatggattg gtcacttaat ttcccataat tttcaaattt acgggtacgg
    12841 taatacacat cacctgaaga ttcataagca tagccttttt caaccaacac ttgaataaat
    12901 gctaaaatat ctggcatatg atccatgact cttggatgca gtgtagcagg ttcgacattc
    12961 aacgctttcg tatcttcttc aaatgctcgg atgaaacgat cagcaacttc tggtgctgta
    13021 atacctaatt cgtttgctgt acggatgatt ttatcgtcta catccgtgaa attcgatacg
    13081 aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg
    13141 ctacgagcat ttccgatatg gatataatta tacacagtcg gtccgcagac atacataccg
    13201 acatgtccct tttgtatagg gatgaattct tctttttttc tggtcaatgt attgtagatt
    13261 tttatcattc tgatctcctc actttttctc ctttgattcg aacgactttt gctggtactc
    13321 ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac ggcacctgca ccgattttgg
    13381 cgttttttcc gatcgttacc ggtcctaata tctgcgcatt cgcagagagc atggctcctt
    13441 gtttaacagt tgggtgtctt ttacccgttt cttttcctgt gccgcctaat gttacgccat
    13501 ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc aatgacgatt cccattccat
    13561 ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg gatctcaaca ccagttagga
    13621 aacgccagaa ttgagcattg atcttcgcca ataaaaagag ccggtgctga tataaaaaat
    13681 gagagaaacg atgccaaaac aaggcatgaa aaccagggta tgtcaaaacg atctcagctg
    13741 tcgaacgtgc agccggatcg tttctctttg ctgcatcaat cgctcgggta aaccaactca
    13801 tgactttcca ctcctttcgt aagaatttta tgtaggaaat catcttctta taaagaaaag
    13861 aagcgccttt taaataagtt tccttatcaa aagacgcttt ccgcgtggtt ccacttttct
    13921 tcataatcaa tgattatctc tgacagctaa cgcctgttat acgttcttgg ctactttgtt
    13981 tcctataaaa cagtttttcc tgttttctgt gtttcgccaa gaccactccc agaggcattt
    14041 cggcaaccat tcattgatac cttgcaccac ccggtatctc tcttgtaatg atcagctgct
    14101 tacttttttc tgttcatcgt gtttagttca gtacttgatt taaatgtgct aatgattttt
    14161 cacgaccaag caattcgatc gtttcaggca attctggacc atgcatttgt cctgatacag
    14221 ctacacggat tggcatgaaa agattttttc cttttacgcc agtttctttt tgaacagctt
    14281 tgatggcagc tttgacagtt ggagcatcta ctacttccat ttgttctagc tgtgctttga
    14341 atgcgtggag aacagtagga actgtttcac cagcaagtac ttcttttgct gcttcattca
    14401 atacaggatg ttcattgaaa aatagcgtag acagatcaac gatttctgca gcatagctca
    14461 tttgaggttg atacaggcta acgatcttct tcacccattc gattgtcttt tcatctgggt
    14521 tttctgctac acgtccatca gcgattagat aaggcagaca catgtcagta aatgcatcta
    14581 gatccattgc tttgatgtaa tggttgttta cccattgcag ttttttcgca tcgaaagctg
    14641 cgggtgattt acttaggcgt tttggatcga acattttgat caattcttgg tgttcgaaga
    14701 tttcgtcttc gcccacaggt gaccagccca ataatgcaat aaagttgaac attgcatctg
    14761 gtagatagcc taattcgcga tattgttcga taaattgcaa gattgattcg tcacgtttgc
    14821 ttagtttttt acctgtttca ctgttgatga tcaatgtcat atgtccgaag gttggtggtg
    14881 tccagccaaa tgcttcatag atcatcagtt gttttggtgt attggcaata tggtcatctc
    14941 cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac cgcaacagca aaattataag
    15001 ttggcatacc atcgcgtttt tggatgacaa agtcgccacc gatattatcg gattcgaacg
    15061 aaatagcgcc tttcaccata tctgtaaatg cgtattctgt attgcgaggc acacggaaac
    15121 gtacaacaga ttctagacct tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg
    15181 cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt
    15241 ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt gctagcaagc agctgatcaa
    15301 tcagcggttg atagatttct ttacgttcag attgacggta agggccgtat tctccaggat
    15361 tttcaggaga ttcatcccaa ttcattccca accaagcaag gttttccaac tggctttttt
    15421 cgccatcttc gatattacgt ttttggtcag tatcctcaat acgaataata aagtctccat
    15481 cattgtggcg ggcaaacaaa taattgaata atgcagttct tgcatttcca atatgcaggt
    15541 gcccagttgg acttggtgca tagcgtacgc gtactttcgt cattttcttc ctcttctttc
    15601 aaactagact ttgactaaca gcaaaaattc cgtcagttac ggcttctcaa atatcattta
    15661 tttaaagcca ctcagcacaa attttacctt ttctaaggta aatagtaaag ccaactggtt
    15721 gaatttcctt accagttggc tgaatttctt ttttattttg attgtttagt ctttttctct
    15781 gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt
    15841 ccagcagctg tttgaagggc acttgttaca acgacttcga tacgttcatt catgtaatgc
    15901 tgtccatctt ctacgacaac catcgtgccg tcatcaaggt aggctactcc ttgttggcgt
    15961 tctgtcccgg cttttactac tagaacattc atcgtttcac cagggatgac gactggtttt
    16021 acggcatttg ccaacgcatt gatattaaga acaggtacat tttggaattc agagacttta
    16081 ttcaagttat agtcattggt tacaatgaca ccatctaaaa gcttagccaa tttgatcaat
    16141 ttgctgtcta cttcactgat atcttcaaag tctccatcgt acatttcgac agatataccc
    16201 tcttcttttt gcaaggcatt taggatatct aatccgcgac gaccacgcac acgtttcaaa
    16261 ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa caaaattagg gatcatcaag
    16321 gttccttcta agaatccagt ctttgtaata tcataaatcc gaccgtcaat gatcacgctt
    16381 gtatccaaga ttttatattt atggaaattt tctcctgatt ttcgttctaa cagttgactc
    16441 tcaacttgac tagctgtttc ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc
    16501 cattcttcga ttcgtgtcgt tcccattctc aatcccagat agcctaagat gatcatcagt
    16561 aaaatcggca atacactatt gacgaatgga atttccatat tgtaaagagg ggtcgagatc
    16621 acaacaccta aagtcaatcc gatgatcgca ccgatcgcac cgaagagaag ataagtcaag
    16681 ctgacctcac ttagtttttg ttctacttgt ttgataatag ctgagatatg tttggccaac
    16741 agcaaagata ataaaaagaa aataagtgcg cctaacaggc tgttggtaaa tgtattattc
    16801 agccatccgt ttgactgttg accgattgcc tgccaagctg agggtaaaaa tgaaataccc
    16861 aagcttgcgc cgacaatgat catcagcaaa gtaatgacac gtttttgcat cgtttttcct
    16921 ccgtttcgac aatcatttat tagttagcgg aacacacgct tcaacgtttc agccaaagtt
    16981 gctactccaa ctatctcgat tccttttggc gcttcccagt tacctaagtt gtttttaggt
    17041 aggtatactt ttgtgaagcc gagtttttgg acttctttga ctcgttgttc aatatgactg
    17101 acacgtctga tttctccagt cagtccaatt tcaccgatga aacattccgt tggctgtgtt
    17161 cctttttctt tatagcttga agcaatactg acagcgatag ctaaatcaat cgctggttca
    17221 tttaatttta ccccacctgc agcttttaag taagcatctt gattctgaag aagcaaccca
    17281 gctctttttt ccagcactgc catgatcaga gatacccgat tgaaatcaag acctgttgtt
    17341 gttcgtttgg catttccgaa catagtaggt gtgactaatg cttggatctc taccaaaatt
    17401 ggacgtgtcc cttccattgc cacaacgata gcagagccag ttgcaccgtc taggcgttct
    17461 tctaagaaaa tctgtgaagg attagcgact tcttccaatc catgctcatg catttcaaaa
    17521 atcccaatct cattcgttga gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta
    17581 tgatgttgtt caccttcaaa atataacacc gtatccacca tatgttctaa cattcttggg
    17641 ccggcgatcg agccttcttt ggtaacatga ccgacgataa aaatggctat cccatttgtt
    17701 ttagcggttt tgagtagttc tgcagtggtt tctcttactt ggctgacact tcctgccaca
    17761 cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa tgataacata atcaggggag
    17821 atatgttcga tggcacgact gatttcgttc atatctgttt cagcgtatag ataaaattct
    17881 gtgttgatac ttccgaggcg ttccgcgcgt aattttatct gctctgcgct ttcttcgccg
    17941 gatacatata agacttttcc gcctatggct gctaattgct gagaaacttg taacagcaaa
    18001 gtggatttcc cgatacctgg atcgccacca atcaagacca tagaccctgg caccacacct
    18061 ccgcctaaca cacggttcag ttcttctaat tttgttttga tccgaggttc ttttttaggg
    18121 acgacgtctg cgatttttgt tggttttgct ttttctcctg tcaaacttgt ccgagttctg
    18181 cggtccgttg tatcttgttc gatttcttcg accatcgtat tccattttcc gcaatttggg
    18241 cagcgtccca agaacttagg cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt
    18301 gatttttttg ccattgtacc gcctcctttt atcctaatca tgagacatta aatcaagctt
    18361 aagtatcttt aacgttttat ttactggaag aaccaaatcc gccttgacgt tgagcagtcg
    18421 tttgatcttg atctgctttt aaaaacggca aaaagatccc ttggccgata cgctcgcctt
    18481 ttttgatcac tacatccttg aaaccaaagt tggtgaattg gaacatgata tggccttcat
    18541 ttgcctcatt attgtaataa tcactgtcaa tcacacctac tccgtttgtc aacactaaaa
    18601 accgcttcaa cggattgctt gaacggttag caagctgcaa aaattcatct tctcccatgt
    18661 aagctttgat tccagtaggc acgagggttg gtttcaattg tttttcattt tctgatacat
    18721 cttttttcaa tccatctgcg actgtttcca accccttttt ccagcctgct ttccagatac
    18781 ttggaatcac gacctcttct gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc
    18841 gctgcggtag attgatattt tgatctttat atttagaaat aacttcaaat cctctttttc
    18901 tcaccggttt tcctccctaa ataaactact cctattctaa catatccgag gctgtaaaag
    18961 actttcttta caaattcttc aagaaattct gctatcttaa agtaaaccat taattcgttc
    19021 tttttccaaa cgactgcaag ttgtacaaaa aaactatttt ttacatgact ttactcgtca
    19081 aaacaagtag aatagaacga aattaatgag tggatatttg attgaggtgt cacatgcgta
    19141 aagaaattaa aaatgcaaaa cgaattattg tcaaagtagg aacaagtaca ttgatttatc
    19201 ccaatggaaa tatcaatctt gcggcaatcg atcagttagc ttttgtcctt tctgatctta
    19261 gaaatcgggg aaaggaagtg atcttagtct catcaggagc tgtaggcgtt ggtatgcata
    19321 aattacattt gtcagaacgt ccgactttga ttcctgaaca gcaagctgcg gcggctgttg
    19381 gtcaagccga actgatgaat atctataatc aaagattcag tgtatataat caacagacgg
    19441 cacagatcct cgtgacaaga gacgtggtag attatcctga aagtcgtaaa aatgtgagga
    19501 atacattgga acagcttttg attatggaca cgatccctat cgtgaacgaa aacgatactg
    19561 tagcagtgga tgaattagat catctgacaa aatttggcga taatgatcag ctgtcagcca
    19621 tcatggcaaa acttgtcgcg gcagaccttt tgatcgtcct ttctgatatc gacgggtttt
    19681 attcagataa tccgactaca aatcccgagg ctcagatgta tcgctatatc gataaaatcg
    19741 atgaaaagat tctggcacaa gcaggtggtg ctggtagcac atttggaaca ggcggtatgc
    19801 agagtaaatt aaaagccgct tctaggatat ttgaagcaaa cagcgcaatg attttagcaa
    19861 atggaaaaaa tccggcaatt atttttgata ttcttgcagg aaaagaagta ggtactttat
    19921 ttaaggagga cttgaaatga ctgacttgat gcaattagga caacaggcaa aagatagcgc
    19981 gcaacaacta gcacttatgc ctacaaaccg caaaaatgag ctacttgttc aaatggcaca
    20041 aaccatcaaa aagaatcaac aagcgatcat tgaagcaaat cataaagact tagaaaaagc
    20101 agtcgaaaac aatatcagtg aaacgatgca agaccgcttg cagctgacac ctgagcgaat
    20161 caatgccatg gctacagaga tcgaaaaaat tgcttcgtta gacgatccta ttggaaaagt
    20221 agatgagatg tggacgaaca ccgacggatt gagaatcggc aaaaagcgcg tgccactagg
    20281 cgtgatcggc atcatttacg aatctcgtcc aaatgtcacg acagatgcag ctagtctagc
    20341 attcaaatca gggaatgccg ttatccttcg cggtggcaaa gaagcattct tctcgaatca
    20401 attattagtc caattattgc agcaagtact ggtcagtgct ggcgaatctc cttatgccat
    20461 ccagtttgtc gatgatactt ctcatgaaac tgcaggacag ttgatgcgat tgacagaata
    20521 cttagatgtg ctgattcctc gaggcggagc aaaattaatc caacgagtca aagaacaggc
    20581 aacggtacct atcattgaaa caggtacagg aaataatcac gtatatatcg ataaagaagc
    20641 acagttatct atggcagtga acatcatcgt caatgcaaaa gcttcccgtc catctgtctg
    20701 taacgcggca gaaacattgc tgatccattc tgagatcgca ccttttttct tgccagctat
    20761 tgaaaaagaa ctggtagaac acggggtctc tttacgtgcg gatgctcgtg cgttagaata
    20821 tctagagacg gctgctttag cagaagacac agattgggat actgaatatc tagattatat
    20881 ccttgctgtc aaagtggtcg attctttgga tgaagcaatt gcacatatca atcggcataa
    20941 tactaaacat tcagaaacga tcgtgacaga tagttatgcg gctagtcaac gtttcttaaa
    21001 tgaagtggac gcggcggctg tctatgtcaa tgcctccact cgttttacag acggatcggt
    21061 atttggtttt ggtgcagaga ttggtatctc tactcaaaag ttgcatgccc gtggtccaat
    21121 ggggttaaat gaattaactt ccacaaaata tatcatttat ggcgatggac aaatccgcga
    21181 ataatgtttc gtgtgaaaca ttattcccgc cttttgcctt ttttctgtaa aggttctatc
    21241 caactagaaa ggtgtcaata tgtttgaaat tttaatgatt ttttttattt accttatttc
    21301 tctgaatatc gctgcttttc tgggagtcgg tattttatcc cttttctttc aattcaaaaa
    21361 acgaagcata gggagtcaac gggaaaaatg gtcgcagtat tttgacaaaa tcgggcaaaa
    21421 agggctggtt acacgcttgc acattagtta catggttgca ttatgcttat tagcgacaat
    21481 caattactat tcttttttcg atcattcaat cgcttatacg attacgctac tgattgctgg
    21541 gatttttcac ttatcttata aatatcagct gaataaaaac cacttgaatc ggactttccg
    21601 ctaaaaaata tccgtacgat aagtagagtg ccttgaagat cggcaaaaaa ctgccggact
    21661 ttttagaggc tgtgacaaag attttgtcac agcctctttt tccatataaa cggcgttcgc
    21721 ctaccagctg cctggtaaat ctgtttcctc ttcgttaaga agcgaaaatc cgtcaaatgt
    21781 ttcgccatca taataacgaa gcagctcatc catggacaag ttcatcgttt tttctttttc
    21841 ttcttccgct gattcagcca gtagctgttc ccgatacacc tgatacatac cagctatttt
    21901 ttgctgcatc gcttttctag tttcaccttc cgcattgatt attggcaatt ctttgatata
    21961 gccacgataa atcaaccgat catctgaaaa ttcaatgggt aattcaacga gtgtcaaccc
    22021 gacttcctga aaatattctt ccagcatcta ttatcccccc attctttttc ttaaatgaat
    22081 tttagcttac ctactgaaaa ttttccagtt tcattatttt gagaaaaaaa gggaatgtga
    22141 caaaaatcgt gtcacatccc ctttttccga ataaatggtg ttcttctgcc aactcctcaa
    22201 tcataagtca caatattcaa caaacatgag aaactgtttt ttgaaattgt tccttgtgat
    22261 tcagagttaa acgacagtct cacaacctct tggtaaacgg cgttcaatca gctgaccctc
    22321 ggtattagct caaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat
    22381 acttaggata aaacgctgat tttacaacct ctgttaaacg gtgttcaatc agctgtcctt
    22441 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat
    22501 actcaggaca aaacgctgat tttacaacct cctgataaac ggtgttcaaa agcttgcatc
    22561 tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt ggctgttctt
    22621 atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc ttcatttgta
    22681 gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat ggcacctgca
    22741 aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat aaatggtgct
    22801 aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt tcgttgcaga
    22861 cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg gaaaaatcct
    22921 gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc taataacagc
    22981 aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt aaacttccgc
    23041 tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca gccaaaaaat
    23101 acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata cgcaagtaaa
    23161 gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag tttatgattg
    23221 cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc agtttcaggt
    23281 aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc aaaaaatgga
    23341 taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc cattccgcag
    23401 ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa aatactgacg
    23461 atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc ggcacgtaac
    23521 ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac aacgatgata
    23581 aacaatcctg tcatcaatgt ttttttcaaa ccgatccgac tagataagaa acctgtaaat
    23641 agcatcacca caccagtgac taacatgtaa ctagtaaata acagggtcgt ttcagctggc
    23701 gtagcgttta actgtgcagc aatcgttttt aagattgggt ctactagacc cactcccata
    23761 aaagcgatca tacaggtagc tgctacaact agcgctgaac ttttcccctt tgtttcttca
    23821 tttgtttgtt cttgtatatc tatttgattc tccaatattt ccatcccttt ctctctatta
    23881 gcatcataca tgatttattt tacatgtcaa tatgcacatg taaaacgtgg aaactttact
    23941 gtgatataat aaagaaaata agacgagagg taggattttt atgaatactt tagcctatat
    24001 attattatgc gtgcttttat ccaaaccacg ttctgggtat gaattgaagc agctgatcac
    24061 aattttttgg gaagcccatc acagtcagat ctatacaacg cttgccaagt tagaccagca
    24121 gggttatgtg accgttttgg acaaagacga gcactctcaa aaaaagatct accacttgac
    24181 tgatgaagga cgtgctcttg tagaggaatg gatcaaagag gaaactcccg ctcctacgca
    24241 aaaagatgaa tttctagcaa aaatctatgc tttttcgacg ctcgataaaa caacagctac
    24301 tggactgcta ttcactagag aacaacagct gaacaaaatc atgcaaaaaa atcaaaaaaa
    24361 attgaatgac ctctcgctag acaagaaaca agaatttgga cgttatgttg tgatccagcg
    24421 cagaattatg ctttgtcagc aggaactcca atggtgtcag caagtaagag aacaaatgca
    24481 gcagtttttt gagtaaggga taaacagaac ctttctagaa atatctattg aagagcaaat
    24541 tatcttcaat aaaagagcaa tacagttgga aaagaaaaag cgatccgaac gacaggccaa
    24601 aacctatcgt tcggatcact atttttctag aactgcattc aagagactat gacaaaaagc
    24661 atgccaaggc cttttttcaa ataacagtgt tccatcattc attactttga attgctttta
    24721 gcacacttct tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg gcaatcacat
    24781 tcattttcca agcattcata taagcagaga ataattgatc aggtattcct tgttccatga
    24841 tcacaccgaa taaagacata catgtcacca ttcctaaaac catcaagcat gaaatagtta
    24901 agatcatttg gaccttgctt tctggattta ctggtagttt aaaggcaatt tttttcgcca
    24961 gcacaccgat gatgaagaca tccaaaatga aagccaccac gaatccaggc actagaccaa
    25021 tcaacaaact tgataaatga agatcttgat gcagccataa attatacgcg ctcatcccca
    25081 agaccatcaa aaaacacatc attgttgtaa aaattattcc ttctttttta tttgtaggca
    25141 atctgtacca ctcctttaaa tttgtcacga aaatcatcgt agcacattta aaaatttctc
    25201 gtaagtactt tttttatttt ctgccctttt atttcataaa cttcatcagc cgtacgctcg
    25261 acaaagtaag aatcatgaga agtaaacaaa atcgttcccg gatatatttg cattagcttc
    25321 tctaaagctt cgatcgttgc catatcaata aaattagtcg gttcatctaa cagcaacaca
    25381 ttacttggct tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc cccacttaat
    25441 gtacataacg gtttcgtcaa agtttcctga gcaaacccta agtgattcag caaactacgg
    25501 acaagcgatt ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga ttcttcagat
    25561 gtcatttgat aacccttttg ctgatatact tgaaagctta ctttcggaga gagaaggatt
    25621 ccttctcctt gtttgcggat atgttctaat aaggttgtct tacctgaacc attttcaccg
    25681 acaatcgcga tttttttacc taaagaaaac tgaaaatcac catttaccag taatgttctc
    25741 ccacttcttt ccaattggac attttgtccc atgatgggat agcggctgtg gagttcaaga
    25801 gattttggta ctggaaaacg gatttgtttc atctgctgcg gcttggttgt ttctcctatt
    25861 ttttggagtc ttctctctaa tgttttcgca ttcttttgga tggctttttg tacactgtct
    25921 ttttgtttag aggaagacaa ccgatccagt ctgatttgtt gctttttctt tttactggac
    25981 actttttcca acttttccgc ttgttttttc ttttcttgga tcgatttttc caatcgtttt
    26041 ttctctttca tgatcgactc tgcttctcgt agctgagtct ttttctccaa ttccttttga
    26101 gtgagatagg cactataatt ccccgaaaac tctcggattt ctccgtcttt tacttcccag
    26161 attttcgatg ctagctcatc tagaaaatag cgatcatgac taacaaaaac cagcgtacca
    26221 taataataac gaagctcttc aatcagtcga tcaatacttt ttctatctag atgggttgtt
    26281 ggctcgtcta gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa ctcgatcttt
    26341 cgttcttccc ctccgctcca tttccgctct gaagtttctt ccagtcttaa ctggaaatga
    26401 ctggctaatt ccaaatttag gtcctttgct tcagcgagat aggttaattg aggcaggtag
    26461 ttgaaggtga tctctgtttg gattttccca tgatcgggaa aaagttcacc ggctattagt
    26521 ttgagcaaag tcgatttacc gacaccattt tctcctatga tccctatacg atcttgctga
    26581 tagacacgaa gagaatcaat agatagttct agctggttgc caaagctgac ttgtagattt
    26641 gttatattta ctgctaaatt ttccatatgt tttacactcc tatttcatgt aaaacaacaa
    26701 ttatcgatac taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat cacatctttt
    26761 cctactctga acgaaataac tatgactaaa tcaccaaatc tgtcatagct ttcacctatt
    26821 ctactgtcgt tcgaaaaaat ggacatactc catccatgaa attgttgttt atacatgtct
    26881 tttgaattta tttagttgtt attcaaaagt tcgaaacgta atttcatcga tgcagtcatt
    26941 tttccgacct ccttttttat taggttacca cacacgattc aaaaaaagca atagctactg
    27001 aatagtaact gaaaaccttt tattcgaaca tagtgatctt ttctggtgct aaacgaacag
    27061 actcgtaacc ttcttctacc gcttttccta ttgataaaat aataactgga acataacggt
    27121 ctttatccaa accaaatgcc tctgccaact gatctgcttc gaatccacca attggatttg
    27181 tttcatagcc atgatcacgc gcaactaaca tgaattgcat agccgctaag ctagaatcga
    27241 ctttgactac atcgttcatt tcttctcttg aaaaactctt gtaataagga atgatcgcag
    27301 ctaattgttg atctctgact tcttttggca tcttccctga ttcgtaagct tggttgtaga
    27361 tttcttcgcc atattcgtaa cattgcatat cgccaaaaat catgaccatt gcagaagaag
    27421 tatcattttg tctcgtattg aaacggatca aaggacgcaa gatttctttt gcttcctcgc
    27481 tttcagcgac cacaaagcgc catggtttca tattgacaga agaaggtgca gtcgtcgctt
    27541 ctttgatcat ttccagcatt tcttcgtgtg ggattttgta tgtttcatca tatacgcgaa
    27601 cggatcttct accagtcata attgttgaaa aatcattttt ttgttccatt attattaacc
    27661 tctttcgttt ctgttgttat attgcaaagt tgggtattca atttatctaa aagtgttaga
    27721 aattgtttca tttctacatc gctgaaagcc ggggatagca cggaacctgt atctttatgg
    27781 tccatttcac aatgcttgat tttcttctgg gctttttctg ttagcgctac atacacttcc
    27841 cggttgttct gaggatttct ctgacgcgaa acatagccat ttgcttccaa taccttcaga
    27901 tgacgagtaa ctgctccttg atcaatctgc aacttttctt gcaaactgct ttgtagcaat
    27961 ggattttctt ctttcagtct catcatgatc tcgtatctag taagacttat accgatattt
    28021 tcttcaaaga tctggacgat tttttgatcg gctaatttga tttgatataa aaggtgacta
    28081 atctcttcta aagacataat ttcctccttt ctaataattg actcgtcaac gattgacttg
    28141 tcaataatag aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata aaaaaaacgc
    28201 tctcaataga aaagtataga gaaaagaaat tttttgtaga atgaacggaa aaaatgataa
    28261 agaaagcaca acgctaaaac caagattatt gattcttatc aaactataga agatagctct
    28321 tctgcagtag ataattttac accgccaaaa atttccgaaa tcgccttttg caagaatccc
    28381 gtcgaatatt agaaattgga tcagtatgct taaacagtaa taaaaaagtt atgacaagat
    28441 gttgtcacaa cct
//
LOCUS       DASJRQ010000026        28229 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJRQ010000026 DASJRQ010000000
VERSION     DASJRQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.026"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            390..1430
                     /locus_tag="U4A80_001052"
     CDS             390..1430
                     /locus_tag="U4A80_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1974685.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            2624..4012
                     /gene="ltrA"
                     /locus_tag="U4A80_001053"
     CDS             2624..4012
                     /gene="ltrA"
                     /locus_tag="U4A80_001053"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1974686.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(4134..5429)
                     /gene="purB"
                     /locus_tag="U4A80_001054"
     CDS             complement(4134..5429)
                     /gene="purB"
                     /locus_tag="U4A80_001054"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1974687.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5442..6566)
                     /gene="purK"
                     /locus_tag="U4A80_001055"
     CDS             complement(5442..6566)
                     /gene="purK"
                     /locus_tag="U4A80_001055"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1974688.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(6559..7047)
                     /gene="purE"
                     /locus_tag="U4A80_001056"
     CDS             complement(6559..7047)
                     /gene="purE"
                     /locus_tag="U4A80_001056"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1974689.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(7463..8770)
                     /locus_tag="U4A80_001057"
     CDS             complement(7463..8770)
                     /locus_tag="U4A80_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1974690.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(8773..9354)
                     /locus_tag="U4A80_001058"
     CDS             complement(8773..9354)
                     /locus_tag="U4A80_001058"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1974691.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(9470..9567)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(9926..11218)
                     /locus_tag="U4A80_001059"
     CDS             complement(9926..11218)
                     /locus_tag="U4A80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1974692.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(11215..11838)
                     /locus_tag="U4A80_001060"
     CDS             complement(11215..11838)
                     /locus_tag="U4A80_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974693.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(11841..12677)
                     /locus_tag="U4A80_001061"
     CDS             complement(11841..12677)
                     /locus_tag="U4A80_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1974694.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(12694..13581)
                     /locus_tag="U4A80_001062"
     CDS             complement(12694..13581)
                     /locus_tag="U4A80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1974695.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(13597..14880)
                     /locus_tag="U4A80_001063"
     CDS             complement(13597..14880)
                     /locus_tag="U4A80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974696.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(15563..16246)
                     /locus_tag="U4A80_001064"
     CDS             complement(15563..16246)
                     /locus_tag="U4A80_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1974697.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(16264..16881)
                     /locus_tag="U4A80_001065"
     CDS             complement(16264..16881)
                     /locus_tag="U4A80_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1974698.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(16891..17715)
                     /locus_tag="U4A80_001066"
     CDS             complement(16891..17715)
                     /locus_tag="U4A80_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1974699.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(17708..18268)
                     /locus_tag="U4A80_001067"
     CDS             complement(17708..18268)
                     /locus_tag="U4A80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974700.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18442..19398
                     /locus_tag="U4A80_001068"
     CDS             18442..19398
                     /locus_tag="U4A80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1974701.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(19703..20659)
                     /locus_tag="U4A80_001069"
     CDS             complement(19703..20659)
                     /locus_tag="U4A80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1974702.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            20763..21365
                     /locus_tag="U4A80_001070"
     CDS             20763..21365
                     /locus_tag="U4A80_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1974703.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(21453..22952)
                     /locus_tag="U4A80_001071"
     CDS             complement(21453..22952)
                     /locus_tag="U4A80_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1974704.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(22968..23492)
                     /locus_tag="U4A80_001072"
     CDS             complement(22968..23492)
                     /locus_tag="U4A80_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1974705.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            23790..24539
                     /locus_tag="U4A80_001073"
     CDS             23790..24539
                     /locus_tag="U4A80_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1974706.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            24530..25315
                     /locus_tag="U4A80_001074"
     CDS             24530..25315
                     /locus_tag="U4A80_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1974707.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            25284..26000
                     /locus_tag="U4A80_001075"
     CDS             25284..26000
                     /locus_tag="U4A80_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1974708.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(26102..27136)
                     /locus_tag="U4A80_001076"
     CDS             complement(26102..27136)
                     /locus_tag="U4A80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1974709.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(27140..28198)
                     /locus_tag="U4A80_001077"
     CDS             complement(27140..28198)
                     /locus_tag="U4A80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1974710.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         8621 a         5867 c         5017 g         8724 t
ORIGIN      
        1 cctaaagcct tgtcttataa gaagaatata atatatacta ctgaaataag gtagtatata
       61 ttactagtaa tggtagtatg ttttatatgc ataaggtagt tcttaattta ttttttgttt
      121 tgacactggt agtacttaat tatctttaat tttaaaacta aattttattc atacgttctt
      181 ctggtagtat ttaattataa ataatattta tttaaagtag ttcttaatga ctttaaaggt
      241 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
      301 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      361 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taattttttt aaggcggata
      421 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      481 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      541 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      601 aactatgtag cgtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      661 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      721 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      781 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      841 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      901 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      961 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
     1021 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttatccctg
     1081 aacgagtatt aaatttaata aaaacatttt cttcaagtta cagtgaagct caaaaaactg
     1141 tccagactat tcataatgca aagaaaaaag ccgaaacaga aagtggtatt tcaataattt
     1201 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1261 aagcctatca aaaattaaaa accgaaaaag ttgaaaatat ccagaacctg atttttgtct
     1321 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1381 tgaattctga aagcttccca aaaactatta cgaaagactg gttagagtga aatttcactg
     1441 acaaattgat tgttgcttca tggagtagca ttaaaattaa aaatttttta ttattctaat
     1501 tatattacaa atgttacaaa gaaatagcta ttttaataga atttatgagt taaaatatta
     1561 tgataaaaat cacaaacgta gctttttacg tcataagtgt taatagttaa taattaattt
     1621 ccaacacaaa aaagagaaca aacgtttgca tgttgctgtt ttttagttat aataatacgc
     1681 gaagagaggt aatttcattt tttaaaaaag ctaaaaacct tgatattact tcttttcagg
     1741 caatacgaat attattcgct tacatttttt tgtataaatt tagttgtttt aaatttaatt
     1801 gtctgctacg cttgtgatga attaattttc gttctgcact tttggacgat aaatttggac
     1861 aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt aaccttcgtc
     1921 gacgtccaca ggtggcatta agacaacctt taatccctta atgcgtttac ctttcagtgt
     1981 tacggtatta cccgcaacgg atttagtata acagatattc aaaagtgata ccactttttt
     2041 tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta agcataatgt
     2101 tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag agggaacgca
     2161 tgtttggatg cgttccctct tttttgttgt gcgccctgca tgggtgaaaa cttggtggtg
     2221 aaagtccact acaggcttgg cagtaggaac tgttagcgaa agacaaggcg gcaatcgtga
     2281 ggttgtggct gaaggaagtc ggacgcaaac tcctgaactg acgaacagaa actagataca
     2341 aggctgaatt aggtcggata agtcttcatc acaagacaaa gtccaatact gcccgaaacc
     2401 tatacagtaa atctagcagt tacatgggag gaaagttttc actcttaccg ggggagatct
     2461 catgagggtg ttgtgggcgt tgaatgaaaa caaaccagca caacgatcat ggcgacatga
     2521 tggtgaatca tgagaagtca gccgaggtca tagtagtaac gacactgacc gttacgaagg
     2581 actgaacaat aagaatcttg aataatttag gaggtgtgag aagatgcgac gaccgcagaa
     2641 aacaagaaaa cttggctatc atcaaagagc tagtgtggaa cacgaagggt atgatgaagt
     2701 gcgcagcact tcttgcggtg aacagaggaa acaagatggt gtagaaaatc tgtttgggag
     2761 aattctagat cggaataatc tcaatcaagc gtatcttcaa gtagtcagaa ataaaggggc
     2821 ggctggtgca gatgggatga ccgtcgatca actgcttcct tacttgaaag agcaccgcga
     2881 ggaattacta tccaatttac gccaagggaa ctataaacct cagcctgttt tgcgagtaga
     2941 aataccgaaa ccaaacggcg gtgtaagaaa actaggaatt cccacggtta ttgatcgtat
     3001 gcttcaacag gcaatcaatc aagtgttaca accgctattt gaaccagaat tctcagataa
     3061 tagttatggg tttcgcccaa atcgcagcgc acatgacgca gtgaagaaag caaaatctta
     3121 ttatgaacaa ggttaccgat atgtggtgga cattgatatg aaagcctact ttgacaccgt
     3181 aaatcacgat aagctgatgt atttcattga gcggaaagta aaggacaaac aagtcctaaa
     3241 actgatccgt caatacttaa tgagtggtgt aatggaaaat ggactcatca caacaactga
     3301 agaaggaacg ccccaaggag gcaatctctc tcccctgtta agtaacatct acctgaacga
     3361 atttgatcaa gtgctagaaa atctaggaca taaatttgtt cgatacgccg atgactgcaa
     3421 tatctatgtt agaagcaaac gagcaggaca gcgtgtgatg aagaaatcca ttcaatttct
     3481 cgaagaacaa ctaaagttga cggtaaatcg agaaaagagt gcggtaggaa gtccattgaa
     3541 acgaaagttt cttggattct gcattctgcc aacaaagaaa ggtattaaaa ttcgccctca
     3601 cgcaaaagca aaacagcgtg tgaaaagcaa gctcaaacaa ctcactaaaa gaaaccgtgg
     3661 aagaagtatc caacttattt taaaagaaat caagcaacta atgactggtt ggatcaacta
     3721 ctatggaatt ggtgagatga aagaattcat gagggagtta aacggttggt tgaagcgaag
     3781 aatcagacaa tatatttgga aacaatggaa gaatccaaga accaaaagaa aaaatctgat
     3841 tcgtttaggg atcgataaag cgaaagcgta tgaatggtca aatacaagaa aaggtatttg
     3901 gtcagtctcc aaaagtcata ttcttcatcg atcattaaca gacaaagaac tggcccgcca
     3961 aggatatgag gacatctcct tgaagtacca gttcgtacac tcaacttatt gaaccgccgt
     4021 atacgggtcc gtacgtacgg tggtgtgaga ggggcggaag gtcagttggc tttcccctct
     4081 actcgattat ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc
     4141 aaacctactc gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca
     4201 tcgtctattt cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg
     4261 cggaactgca cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct
     4321 tcttctcttg tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa
     4381 ccaaatgttg cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg
     4441 atattgccaa aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt
     4501 cgttctgctg aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca
     4561 gtgaccatgt gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga
     4621 ttccttttgt gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact
     4681 tcacgtgttt cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta
     4741 gcaataagcg ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta
     4801 gagatttctt gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg
     4861 atatttgcga acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca
     4921 tgttcaaatc gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca
     4981 aaagtagttg gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt
     5041 tcttttgctt tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca
     5101 tttgcttgct tgatgagata gccataagca gtatctacga catccgtact tgttagacca
     5161 taatggaccc attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg
     5221 acatcatggc gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc
     5281 tcacgaattt ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct
     5341 gctaaaatct cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc
     5401 atttcagggc gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt
     5461 tgtttgattt cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc
     5521 ttgcgacctt tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc
     5581 tcaatcaagc gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg
     5641 cttatcaaac gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg
     5701 aactgactca tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct
     5761 aattcattca catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct
     5821 gaaaggctca ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc
     5881 tgatcaatcg cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc
     5941 acgaccggaa aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct
     6001 ttttcaaacg ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca
     6061 ggagctagat ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga
     6121 gtggttttca aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga
     6181 ctgacgatcg ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct
     6241 aacaggcggt cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt
     6301 aaaatcgcat ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt
     6361 cttctggcaa gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct
     6421 gcaacttgtg cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt
     6481 tcttttgcac taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt
     6541 gaaccaggca acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa
     6601 cattgaacgt ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg
     6661 tatcgcaagt agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg
     6721 tactcctcca ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga
     6781 ttggaccggt acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg
     6841 cgcggctccc cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc
     6901 atattcaaac atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac
     6961 accgaattca tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc
     7021 cataatcaca gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt
     7081 ttaacaatca ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt
     7141 attgttcgtg tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct
     7201 ttttatggat aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca
     7261 aacatgagaa gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca
     7321 cgacctcttc cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg
     7381 caaaatacga ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt
     7441 tcacaacctt tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt
     7501 ttttcaatcc attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc
     7561 catttccagt gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat
     7621 tgaaacccag cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga
     7681 aatcaacttc caacagcata cggattcctt ggactgccac catcccgaac atgactaaca
     7741 ttcctccacc aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata
     7801 atcctaaaac aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg
     7861 aaagttgaac caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac
     7921 cgcctaagat aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa
     7981 catttttccc agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga
     8041 ccatggaaat gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat
     8101 aaaagggttg tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc
     8161 ccatgaaagc agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa
     8221 tgaacccttt tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta
     8281 gcagatttgt tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga
     8341 ttgctacagg aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag
     8401 ggaatagttt tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag
     8461 cgataataga tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag
     8521 ctactgcttg gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa
     8581 ctgtcaattg caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag
     8641 tcatctgttc ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc
     8701 ctgcatacat cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt
     8761 ctttttccac gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc
     8821 aatacgcgca agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg
     8881 gaaagacttt tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa
     8941 ttcgatcaat ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt
     9001 gtcttctgaa gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa
     9061 agaataaaca gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc
     9121 gaagatcatt ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc
     9181 ttcaatggtc acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc
     9241 catttgttcc atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac
     9301 gccctcgcct aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt
     9361 tgctccttta tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt
     9421 tctgttaaag aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc
     9481 ttttacttat agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga
     9541 tatataagta ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg
     9601 cacgtgaagg cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt
     9661 ctaacactac tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga
     9721 tttttaatgc attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc
     9781 taaaaaagca gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc
     9841 cgaataaata gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg
     9901 ggaagtaatt tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta
     9961 ctgcttttcc tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac
    10021 catctacgtg agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat
    10081 catctaataa acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct
    10141 catatggctc accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat
    10201 cctttttatc tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc
    10261 ctttgctgcc attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat
    10321 acgtcaaaat cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga
    10381 tatcatcgga aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat
    10441 acatttctcg caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta
    10501 gttcgcttcc atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca
    10561 tcccataaaa gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa
    10621 aattcactag atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct
    10681 tatttcgatg ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga
    10741 agtggataga aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt
    10801 gatacggtgc ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga
    10861 ccgttattat ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat
    10921 catatccttt tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa
    10981 taatgacttc agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg
    11041 tctttacttt tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg
    11101 tttgagatac atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg
    11161 cctcataaaa aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt
    11221 aaagtcattc tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag
    11281 taatgcagtg agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa
    11341 ataccacaga aaccccttga cgttttttga caacagccag atattccttt tgatcaaata
    11401 aacaagacta tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg
    11461 ggatacaaaa agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc
    11521 cactaataaa taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat
    11581 gaaacacatc cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg
    11641 gacagataga taataagaag gatacatcat caaaaaataa atcagataga caacacgagc
    11701 agacagcact aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact
    11761 gataagactg gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc
    11821 ttctattggc tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga
    11881 tatttattga agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca
    11941 aacatactcg cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat
    12001 accgtgtaag atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc
    12061 caataaaact ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt
    12121 ttggtaaaga cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg
    12181 gggatgctga ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt
    12241 ggtaaaatca gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc
    12301 ggaatcaaaa tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat
    12361 ctttttcctt tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact
    12421 gtccctatcg tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa
    12481 attccagcaa accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc
    12541 aaattcagcg aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat
    12601 aagaccggat aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc
    12661 gttactgtat tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga
    12721 tttgaaaatc agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact
    12781 agcatacccc atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt
    12841 cgtggcatca ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc
    12901 cgcgattgtc tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat
    12961 cttgtaaaac gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc
    13021 tgggatctct ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga
    13081 catcaaaaca agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc
    13141 gcctaatcgt tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac
    13201 tagagcgata gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa
    13261 agtcgttcct ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt
    13321 cacaggtaca gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc
    13381 atcactcagc atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg
    13441 ataatcggtg aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa
    13501 tcctaggatg aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg
    13561 ttttagtttt tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa
    13621 ctcataaaga ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac
    13681 taaaagattg tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc
    13741 gccgttgtcc atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc
    13801 atctgcacga gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt
    13861 taaaatttca ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc
    13921 tttatgggct gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga
    13981 ttctccatct gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc
    14041 ccctgacaat atttctgtca actggttact tgatagaaac accattgccg cttctctatc
    14101 cctaaccgcc tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg
    14161 ggcccctttg tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt
    14221 ttcaaatccc aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac
    14281 ttgtggtatt tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat
    14341 tcctaattct tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga
    14401 agctggttca gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga
    14461 attcaccccg ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga
    14521 ttcatctgca ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat
    14581 ctgattgatg tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga
    14641 tgctgctagt gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat
    14701 cgtcacatgg ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg
    14761 gacttgcgaa ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca
    14821 agccgtcaag aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat
    14881 tctctgcacc tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata
    14941 tttattaaac gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct
    15001 aggatgaaca actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc
    15061 taaaaacact tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag
    15121 acaactgccc aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc
    15181 ctaaaaaaca gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga
    15241 tgcacaagca ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg
    15301 acaatttagc tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc
    15361 tatagaaagc gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa
    15421 ggttttaact cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt
    15481 gaattctgaa caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc
    15541 ctcgtctttt gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt
    15601 ccattttacg actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc
    15661 ctgcttgaat ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa
    15721 gttcaggata ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga
    15781 tattgataat cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta
    15841 ccgtactgat aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg
    15901 taggaccgtt caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg
    15961 aagaacgagc accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg
    16021 cctcaataat gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg
    16081 cattcgcatt tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca
    16141 attttccaaa tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat
    16201 cttttctcaa ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct
    16261 ttgctatctc cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg
    16321 cgcataaccg gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata
    16381 gttggtggcc gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa
    16441 cagttgtgta tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg
    16501 gatagttatc cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc
    16561 cccataagcc ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca
    16621 atttgattct aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg
    16681 tagttcttgg gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt
    16741 ttcttgctga ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa
    16801 gagtatcact gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt
    16861 tttccacttt tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc
    16921 ttctagcgtc aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa
    16981 gtgccaaggt tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat
    17041 aaatccatac tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact
    17101 tgcatccaat acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc
    17161 cagacctgtg tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt
    17221 cgcctcctct tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac
    17281 ttgttgttgg gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc
    17341 ttcagcagcc ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc
    17401 gttacttaat gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg
    17461 accaaccaaa actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg
    17521 atcttttgtt tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact
    17581 agcagttgtt ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc
    17641 atcatcgcta aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc
    17701 taaaaattta ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat
    17761 gaacgtaagt ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt
    17821 cgatctgttt ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt
    17881 catcatattg aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca
    17941 aagggatcag ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt
    18001 tgatttgctt gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac
    18061 gcaaaatctt ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca
    18121 tcgcttcaga tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga
    18181 tcagcaagta ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca
    18241 taataagatc aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa
    18301 gttttgatca aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc
    18361 tctcctcttt attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca
    18421 actatgaagg agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc
    18481 atcgcacatg agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct
    18541 tccagaactt cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac
    18601 ggaagctatc aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca
    18661 aatagacagc atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt
    18721 gaaaaagcca tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa
    18781 aagaatttga tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta
    18841 cgctcaataa tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc
    18901 agttacaaag aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct
    18961 ctgttggata tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct
    19021 gaagtagttg cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact
    19081 atcttgcgta acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg
    19141 ccaaaagtcg gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt
    19201 gctgatcgtg cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat
    19261 acatctgaag caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg
    19321 cccaatcgct ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt
    19381 ctatggaaaa atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa
    19441 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga
    19501 ggaaagcttt tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc
    19561 acaaggagca atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
    19621 aggagttgac aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga
    19681 atattagctg aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga
    19741 tttcaataaa acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt
    19801 cagggcgact agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg
    19861 ttaaaatttg tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac
    19921 catagatatg ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt
    19981 ctttaatcac attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct
    20041 gtctgagcca gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg
    20101 tcagtaaatg ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg
    20161 ccatttgtct caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct
    20221 gcttcgtacg atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca
    20281 ttaatcccgc ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag
    20341 tgaccaaaac cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga
    20401 tgaacaggaa taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga
    20461 acagtgctgt tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg
    20521 tagagcgctt ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca
    20581 acagcgataa gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg
    20641 ttcgcattcc tatacgcatt tacttccccc ttttttattt taattatact tgaataatga
    20701 aaaactagct atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct
    20761 ctatgcaatt tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt
    20821 ttttatgtat cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat
    20881 ctgctgtttt ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga
    20941 tcatcggcaa tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt
    21001 ttcacaatga ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg
    21061 tcgtgtcaga tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc
    21121 tgattgcgtt gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag
    21181 atacttttat agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa
    21241 cagaaaagaa ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc
    21301 taagaacaaa tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg
    21361 actaattttc ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct
    21421 ccatttttga ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct
    21481 gcctcttttg gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc
    21541 tctacgattt ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa
    21601 agcaaatttg tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt
    21661 cctgtatcga caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc
    21721 aattgcggta gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag
    21781 gcattggcga tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg
    21841 gcttctgctt gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca
    21901 cggaatcgtt ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt
    21961 tctttagcgt accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca
    22021 cgacgtgtga tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc
    22081 tgcatttcct gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg
    22141 tctgcttttg ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt
    22201 tcttttaatg attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt
    22261 tctgcttgag cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca
    22321 tctcttttga cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct
    22381 ctgacctctt tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa
    22441 gcgacttctt gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc
    22501 atagaaccaa gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt
    22561 tcttctcttg tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa
    22621 ccgattttga tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact
    22681 tcaggggtgg agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa
    22741 actggcaaga cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca
    22801 atgtgcacat ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt
    22861 gctgtctggt acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt
    22921 gcgatcgcga taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc
    22981 atgctttcat aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc
    23041 gttccccgct cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa
    23101 ccggtaacac ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca
    23161 acagctactt gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc
    23221 atcggcatca atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta
    23281 gcaataaata aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag
    23341 cttgtgaaag ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat
    23401 acatctccaa aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa
    23461 taaatcgtct caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct
    23521 atcataccaa tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta
    23581 taggaaggtt gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt
    23641 cacaataaat tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc
    23701 tccagaacac gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg
    23761 ccatattgat caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct
    23821 ctagcattgc taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg
    23881 acccgacaca tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt
    23941 ttatcgttgt catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg
    24001 gtcttccttt ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct
    24061 ctgaaaaaga aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc
    24121 aagagattat tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca
    24181 aagatggaca aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag
    24241 gcgccgtttt tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa
    24301 tagcagaaca gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag
    24361 ccatctcttc tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc
    24421 aaacagagca gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt
    24481 tttgtttaaa gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa
    24541 tgggatagaa actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc
    24601 agtcgcaact ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac
    24661 gaaacaatat ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt
    24721 catttctggt ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct
    24781 aaatcaaaat tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat
    24841 tgccggtttt ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt
    24901 agaaagcgat tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca
    24961 agtgttaggg aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa
    25021 cggacaggtc cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt
    25081 ctgcgatgac ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt
    25141 ttcgacgaag ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg
    25201 ccacggtgga tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga
    25261 attaccaaaa aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa
    25321 tggttgcaga attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa
    25381 gtttcagaaa tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc
    25441 tgcttgatgt atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc
    25501 aaggaaatga tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga
    25561 atgtgatccg ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc
    25621 agctggcgat cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg
    25681 atcagaaatt gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat
    25741 tcatcgattt gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc
    25801 taaaagaaga tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta
    25861 tatccgtcaa gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca
    25921 aatatttgga aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa
    25981 agctcacaga ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag
    26041 aagtaacaac tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt
    26101 cttatcttaa ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta
    26161 ttatttcacg aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat
    26221 ttgaacctac atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag
    26281 cagggtctgg aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg
    26341 caaaaacatc ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt
    26401 cattatcttg aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag
    26461 ctaaaaatcc ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac
    26521 tgacaatatc gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag
    26581 cagcttctgt ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa
    26641 cagcttccgt caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt
    26701 cacgactaaa catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat
    26761 acgcagtatt tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg
    26821 gcgaatacga tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga
    26881 tatgattgac tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg
    26941 caagattggt ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg
    27001 ctgacacgat attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa
    27061 tattcaattt ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca
    27121 tactttcttc ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa
    27181 cgatccatac aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg
    27241 ctaaaagtgc ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg
    27301 tagccatcat gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg
    27361 ccgtcaaaac tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac
    27421 tgtaatacca tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg
    27481 cgccgattgc tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcgactgaa
    27541 atagccacgg tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg
    27601 cacctgtaac tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa
    27661 aagtagcgat aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca
    27721 agccactcat ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa
    27781 ttgaggaata catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa
    27841 aggtcgtaga accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc
    27901 ttaagaaaaa aggttttgaa actagaataa gaataatata aataactaat ccccaaatca
    27961 agacattgat tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg
    28021 ccagtgctgg caaagccact gttaagacga ctttcgatag aatttttcca acagattcag
    28081 aaaaaatccc cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga
    28141 ttgcactgac aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct
    28201 ttctcctcct aattatgttc atttattca
//
LOCUS       DASJRQ010000027        28105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJRQ010000027 DASJRQ010000000
VERSION     DASJRQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.027"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..519
                     /locus_tag="U4A80_001078"
     CDS             <1..519
                     /locus_tag="U4A80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974711.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            527..1042
                     /locus_tag="U4A80_001079"
     CDS             527..1042
                     /locus_tag="U4A80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1974712.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="U4A80_001080"
     CDS             1147..1977
                     /locus_tag="U4A80_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN1974713.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="U4A80_001081"
     CDS             1994..2845
                     /locus_tag="U4A80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1974714.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="U4A80_001082"
     CDS             2842..3201
                     /locus_tag="U4A80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN1974715.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="U4A80_001083"
     CDS             3216..3377
                     /locus_tag="U4A80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1974716.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="U4A80_001084"
     CDS             3374..3679
                     /locus_tag="U4A80_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974717.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="U4A80_001085"
     CDS             3679..3996
                     /locus_tag="U4A80_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN1974718.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="U4A80_001086"
     CDS             3993..4388
                     /locus_tag="U4A80_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974719.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="U4A80_001087"
     CDS             4385..4723
                     /locus_tag="U4A80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974720.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="U4A80_001088"
     CDS             4720..5088
                     /locus_tag="U4A80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974721.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="U4A80_001089"
     CDS             5085..5390
                     /locus_tag="U4A80_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974722.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="U4A80_001090"
     CDS             5414..5617
                     /locus_tag="U4A80_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN1974723.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="U4A80_001091"
     CDS             5614..5910
                     /locus_tag="U4A80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974724.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="U4A80_001092"
     CDS             5986..6402
                     /locus_tag="U4A80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1974725.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7310..7654
                     /locus_tag="U4A80_001093"
     CDS             7310..7654
                     /locus_tag="U4A80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1974726.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="U4A80_001094"
     CDS             7659..7940
                     /locus_tag="U4A80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974727.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="U4A80_001095"
     CDS             8043..8357
                     /locus_tag="U4A80_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1974728.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="U4A80_001096"
     CDS             8335..10029
                     /locus_tag="U4A80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1974729.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="U4A80_001097"
     CDS             10049..11227
                     /locus_tag="U4A80_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1974730.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="U4A80_001098"
     CDS             11190..11876
                     /locus_tag="U4A80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1974731.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="U4A80_001099"
     CDS             11876..13036
                     /locus_tag="U4A80_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1974732.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="U4A80_001100"
     CDS             13046..13921
                     /locus_tag="U4A80_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974733.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="U4A80_001101"
     CDS             13918..14229
                     /locus_tag="U4A80_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974734.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="U4A80_001102"
     CDS             14219..14572
                     /locus_tag="U4A80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1974735.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="U4A80_001103"
     CDS             14562..14963
                     /locus_tag="U4A80_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974736.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="U4A80_001104"
     CDS             14956..15360
                     /locus_tag="U4A80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974737.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="U4A80_001105"
     CDS             15372..15980
                     /locus_tag="U4A80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1974738.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="U4A80_001106"
     CDS             16000..16362
                     /locus_tag="U4A80_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974739.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="U4A80_001107"
     CDS             16365..16547
                     /locus_tag="U4A80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974740.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19897
                     /locus_tag="U4A80_001108"
     CDS             16565..19897
                     /locus_tag="U4A80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1974741.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19948..20685
                     /locus_tag="U4A80_001109"
     CDS             19948..20685
                     /locus_tag="U4A80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1974742.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20695..22986
                     /locus_tag="U4A80_001110"
     CDS             20695..22986
                     /locus_tag="U4A80_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1974743.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23010..25091
                     /locus_tag="U4A80_001111"
     CDS             23010..25091
                     /locus_tag="U4A80_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974744.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
     gene            25105..25452
                     /locus_tag="U4A80_001112"
     CDS             25105..25452
                     /locus_tag="U4A80_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974745.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            25445..25585
                     /locus_tag="U4A80_001113"
     CDS             25445..25585
                     /locus_tag="U4A80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1974746.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            25655..26074
                     /locus_tag="U4A80_001114"
     CDS             25655..26074
                     /locus_tag="U4A80_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1974747.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            26071..27081
                     /locus_tag="U4A80_001115"
     CDS             26071..27081
                     /locus_tag="U4A80_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1974748.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            27271..27344
                     /locus_tag="U4A80_001116"
     tRNA            27271..27344
                     /locus_tag="U4A80_001116"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27305..27307,aa:Ile,seq:cat)
BASE COUNT        10059 a         4237 c         5992 g         7817 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaaatcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcgaatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    19441 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    19501 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    19561 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    19621 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    19681 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    19741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    19801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    19861 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    19921 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    19981 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    20041 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    20101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    20161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    20221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    20281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    20341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    20401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    20461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    20521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    20581 aacaaaatga agaccatgca atatccaacg ttgcaagtgg atattaatca ttttaattgt
    20641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    20701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    20761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    20821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    20881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    20941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    21001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    21061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    21121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    21181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    21241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    21301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    21361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    21421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    21481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    21541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    21601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    21661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    21721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    21781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaatggt
    21841 ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt
    21901 caaaatggtg gttacgtttt attagatcct aaagaagccc ctagtcgtat tttgattatg
    21961 gacacaccag ataagaatac tgcacggaac gttttgcaaa tcaacaacgc tggtattggt
    22021 ttctctaaaa ctggcattaa tggaacgtat gacaccgcat ggacgttaga tggcggattc
    22081 aatgcctcgt ttattacagc tggtgagata gtagggatta ctattagagg tactacatta
    22141 attagtgatg gcactgatta tagaacaagt atagctaatg gcaaaatgac ttggtactca
    22201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    22261 ggcattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    22321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    22381 ccaaatttca gatacagtaa tgctagttat ataaatgatg gtgacggtaa ttctttaggt
    22441 attgatggta gtgcaggtaa ttcatgggaa tttaaagtag ctggtaggac tatgaaattt
    22501 actagtgacg gtatgctaac gttgccaggt tgtttttttg gttcatggga agatgggaaa
    22561 cttgctagat ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    22621 aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    22681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg taaagttcgt
    22741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    22801 acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca tttcatcatt
    22861 gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg actagggtat
    22921 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    22981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    23041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    23101 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    23161 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    23221 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    23281 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    23341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    23401 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    23461 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    23521 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    23581 actccaacag catcgacttc gggggctatg attatagtgg gaatccgaat ttaatgtcca
    23641 aactaaaatc gagcgatttt aacgttggtt accacgggtc actaactaat gttaacggaa
    23701 tgttacattt tatgtctgat ggtacagggg acattgatat gtttacgcgt attaatacac
    23761 ctcagcttgc tagtggcaaa acatatactc tgagtgcaaa agttcgattt gatgaaggaa
    23821 ctacaggagc tattaataaa ttacgtttgg tatatcgtac atcaccagga gaaaatatat
    23881 tattggaagc aaataataca actattacaa tagatgatgt agggaaagaa ataacaatca
    23941 aaggcacagc taacgttaat tatcaaatca caaatttaga tcgtttttat attagtgtta
    24001 gtttttctaa cagggataaa ataaatggtg gatttaaatt gtacgacatc aaaatcgaag
    24061 aaggctcaac agccactccg tatcagccaa acttactaga tgacccttac tgggtaggta
    24121 aaacatcgtt gggtaagaat attgcaaata aaggtacgac gttcccaatt aaatctagcg
    24181 cgtacgaaat atataaaggt aacacggaag aagagcttat aataggtcaa acgtatacta
    24241 tcacgcttaa aggaacaaaa cccgcaagtc aaacctttgt agcatataat tattggaacg
    24301 ttaattttgg agatctaaaa ccagttgagg gattgacaga cgtatggtct ctaacattca
    24361 caccaacgaa acttgaaccg ggtttgccta aagaccttcg catttttcag tcgcctaaag
    24421 aaacggccgg cgcatgccga attgactggc tcaagatcga aaaaggcgac acacgaaccc
    24481 cgaatattga gcaatataaa taccgaggaa tcggcatgcg agactcaaac aatccaaaag
    24541 attatgtatg ggatctagaa cctagctatg ttgagaataa cctagctaca gatatcaagg
    24601 ttgctgaaat cacaggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    24661 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    24721 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    24781 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    24841 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa
    24901 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    24961 tggttggctc gatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
    25021 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
    25081 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
    25141 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
    25201 ccaccagttc ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta
    25261 gctactgaag aagagaagag tggcacagcg gttgacgata ttgaagatgt cgatcagttg
    25321 aagcaattaa atgctctact gacaaaacaa ttggcgatat cggtacagga acaagaaaaa
    25381 atgcaacaaa tgttagctca attgacgatg gaagtcgcaa gtattaagaa tggagggaaa
    25441 agtaatgaat agttttccgg gctttgaaaa tataaaacaa ttttatgatt ggggatgtta
    25501 tacggaccaa gatttacttg actatgtaaa catgaactgt ttaacaaaag accaatataa
    25561 gcagattact ggaaatgaaa tttaattaag cacaaagtta aataaaaagc gtactcaaac
    25621 gagtgcgctt ttttgcataa aggagaaata gatattggaa aaatatttaa atacattctc
    25681 agtaatgaca ggggtgatag gcgggacaat tgttggttta ttgggaggga tggacaatat
    25741 actacatgtt ttaatttttc ttgtgggtgt ggacttctta actggacttg ctaaggcatg
    25801 gaaattgaaa gaaataagta gtgagatagg tttcgaagga ttgttgaaga aagtcttaat
    25861 cttcgttgtg attgccgttg cggttgaagt acagaaaatc gttgggaatt ctatacctct
    25921 aagagaaata gttattatgt tctatgttgc aaatgaaggg attagttttt tagaaaacat
    25981 ttcggtattc cttcctttgc cagataagtt aaaagaggta ttccaacaaa tacgaaatga
    26041 tactgaaaat aaagaccgag gaggaaccaa atgaaaaaga aaatctttgt aggagctatt
    26101 atagctcttt ttttattgcc aataaacgcc tttgcttaca cgatcaacaa tgaattcaat
    26161 ttgggcgcaa acgaaggtag ttctcaagta gcaaataacc aatacatcct attgcatgaa
    26221 acagcaaacg aaactgcgac aggacgaaat gaagcgcagt acatgaaacg ttcttggtac
    26281 aatgcctata cagcgtatat tgttggcgat ggtgggatcg tttaccaagt tggacaacct
    26341 ggttatgtac aatatggcgc tggttcatat gctaatgcaa atagtcctgt gcaaatcgaa
    26401 ctgcaacata cgcacgataa agtaacgttt gaaaagaact acaaggcata cgttgaattg
    26461 gctagagatt cagcaatgaa atatggtatt ccattaacat tagaaacgcc ttataaccaa
    26521 ccaggaatca aatcgcattt atgggtaaca caaaatatct ggggcgatca tacagatcct
    26581 tacggttatc tttctgaaat gggcgtaagt aaagaaaaat tagcatatga tttagctcat
    26641 ggatttaccg atgaaaatcc gacaacttca gatgataaac cagtcattga tccaactaga
    26701 gcaggtgcag caaatcctac actgacagat ggaacaaatt acgcccacat tgatcagttt
    26761 ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc acatcgctaa ctataaatac
    26821 gagtatattt tcattatgga ctacaatact gggaaagaat tagctcgagt aagagctgat
    26881 ggaatttata gaccagatgt aaaccaagct tataatactt ctggaaatgt tggttatcat
    26941 gtatctttca atatgcgtaa ttttcctagt aagaaagtct atgtaatgat gcgggcaacg
    27001 aatgatccag agggaaacac taaaggcggt gcgcaagatt tccatgacaa acgttggtat
    27061 ttaaatattc ctaaacgata aaaatagctc ctcgttgagg agcagtacat aactatattg
    27121 acaactataa aaattattcg ataaaatagt gatgttatcg catatcttca ctatcaccca
    27181 taaatagtca cactccaagc tatgcgataa caggtttgtt gccacacatt ctactggttg
    27241 attgtttatg gctttatgtg gcaacaacca gtacccttag ctcagttggt cagagcagac
    27301 ggctcataac cgtccggtcg taggttcgag tcctacaggg tacattaacg tagccatttg
    27361 aatcgttctg tgttagaatt tttttgaaga gtattataca agctaaagct tttcttcatt
    27421 gccactcaaa tgagtggctt ttttatgtat ccttttatgg attaatgaaa ggatgtttca
    27481 catagttata cttctgtata tttgaaaagt tttactttga tttttaaata gaaagacatt
    27541 tgggttaaat tgtgagataa taataaagaa gagtttaaag cgcaccccaa accacttccc
    27601 cataagtgtg ttacgcttta aactctttta tatttgaagc cattaaaaag cataccatat
    27661 ttttgaaaaa aagtgagaaa aaaggcttac aattggagtg gtagttaatt agtgacttat
    27721 ttttgatttt atagcactga tactataaaa tatagatatc atcatattac acaatcttaa
    27781 tactaactta aaaaatatct cctttcacaa gtatggtgat aaaattcgtt ccgggctacc
    27841 tttttaggta gcctacttta atctttatac ctttctggat caacgaaagt atactttata
    27901 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcatgaa tcggctacac
    27961 tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc
    28021 atgtcaagac gtcaacgaag aacctattca aaagaattca aacaacaaat cgtcgatctc
    28081 tatctcgctg gtaagcctcg cgcag
//
LOCUS       DASJRQ010000028        27925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJRQ010000028 DASJRQ010000000
VERSION     DASJRQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.028"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(362..1306)
                     /locus_tag="U4A80_001117"
     CDS             complement(362..1306)
                     /locus_tag="U4A80_001117"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1974749.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1602..2546)
                     /locus_tag="U4A80_001118"
     CDS             complement(1602..2546)
                     /locus_tag="U4A80_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974750.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2767..3981
                     /locus_tag="U4A80_001119"
     CDS             2767..3981
                     /locus_tag="U4A80_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1974751.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4003..6018
                     /locus_tag="U4A80_001120"
     CDS             4003..6018
                     /locus_tag="U4A80_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN1974752.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6101..7474)
                     /locus_tag="U4A80_001121"
     CDS             complement(6101..7474)
                     /locus_tag="U4A80_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1974753.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7500..7784)
                     /locus_tag="U4A80_001122"
     CDS             complement(7500..7784)
                     /locus_tag="U4A80_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1974754.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7786..9801)
                     /locus_tag="U4A80_001123"
     CDS             complement(7786..9801)
                     /locus_tag="U4A80_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1974755.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9980..10285)
                     /locus_tag="U4A80_001124"
     CDS             complement(9980..10285)
                     /locus_tag="U4A80_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1974756.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10374..11405)
                     /locus_tag="U4A80_001125"
     CDS             complement(10374..11405)
                     /locus_tag="U4A80_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1974757.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11612..12487
                     /locus_tag="U4A80_001126"
     CDS             11612..12487
                     /locus_tag="U4A80_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1974758.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12639..13481)
                     /locus_tag="U4A80_001127"
     CDS             complement(12639..13481)
                     /locus_tag="U4A80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1974759.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13573..15450)
                     /locus_tag="U4A80_001128"
     CDS             complement(13573..15450)
                     /locus_tag="U4A80_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1974760.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15447..16868)
                     /locus_tag="U4A80_001129"
     CDS             complement(15447..16868)
                     /locus_tag="U4A80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1974761.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16871..20260)
                     /locus_tag="U4A80_001130"
     CDS             complement(16871..20260)
                     /locus_tag="U4A80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1974762.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20517..21908
                     /locus_tag="U4A80_001131"
     CDS             20517..21908
                     /locus_tag="U4A80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974763.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21981..22628)
                     /locus_tag="U4A80_001132"
     CDS             complement(21981..22628)
                     /locus_tag="U4A80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1974764.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22636..23322)
                     /locus_tag="U4A80_001133"
     CDS             complement(22636..23322)
                     /locus_tag="U4A80_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974765.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23328..24401)
                     /locus_tag="U4A80_001134"
     CDS             complement(23328..24401)
                     /locus_tag="U4A80_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974766.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24623..25297
                     /locus_tag="U4A80_001135"
     CDS             24623..25297
                     /locus_tag="U4A80_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974767.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25302..26642
                     /locus_tag="U4A80_001136"
     CDS             25302..26642
                     /locus_tag="U4A80_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1974768.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26791..27777)
                     /locus_tag="U4A80_001137"
     CDS             complement(26791..27777)
                     /locus_tag="U4A80_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1974769.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8152 a         5261 c         5058 g         9454 t
ORIGIN      
        1 tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg tcgtttaatt
       61 gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt agatatgaat
      121 cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca ttgataacga
      181 ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga tacattttcg
      241 gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca ataaaaaact
      301 gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt
      361 cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt
      421 tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg
      481 atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag
      541 gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa
      601 taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat
      661 cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca
      721 ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac
      781 ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac
      841 ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag
      901 attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac
      961 cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt
     1021 taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga
     1081 tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc
     1141 aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga
     1201 tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa
     1261 caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc
     1321 ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac
     1381 atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat
     1441 aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga
     1501 actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta
     1561 aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat
     1621 tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta
     1681 tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac
     1741 gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc
     1801 aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta
     1861 caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat
     1921 ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt
     1981 gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc
     2041 atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt
     2101 gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg
     2161 cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt
     2221 aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc
     2281 acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta
     2341 tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg
     2401 tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt
     2461 gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga
     2521 gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact
     2581 ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg
     2641 caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt
     2701 ccgctattga gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa
     2761 aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt
     2821 ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg
     2881 cgttctttat ctgctgttga attattaacg actgctccct cttttatggt cactattttt
     2941 gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga
     3001 ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc
     3061 ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc
     3121 atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca
     3181 tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt
     3241 tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg
     3301 tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa
     3361 gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg
     3421 gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac
     3481 gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt
     3541 tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg
     3601 atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt
     3661 gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt
     3721 atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat
     3781 ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt
     3841 aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc
     3901 cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa
     3961 gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat
     4021 aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat
     4081 ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat
     4141 cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg
     4201 atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg
     4261 acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca
     4321 gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt
     4381 attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat
     4441 caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga
     4501 atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac
     4561 catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat
     4621 tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt
     4681 cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa
     4741 tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc
     4801 tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc
     4861 aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat
     4921 cttttttttg acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa
     4981 atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa
     5041 aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa
     5101 tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca
     5161 aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa
     5221 caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa
     5281 acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg
     5341 aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat
     5401 gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt
     5461 gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca
     5521 cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag
     5581 acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg
     5641 gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat
     5701 ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta
     5761 ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa
     5821 cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt
     5881 tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg
     5941 aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat
     6001 tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt
     6061 cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa
     6121 caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag
     6181 cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc
     6241 ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata
     6301 ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata
     6361 ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga
     6421 aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg
     6481 atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg
     6541 gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc
     6601 cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa
     6661 acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc
     6721 ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg
     6781 acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct
     6841 ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag
     6901 cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca
     6961 taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta
     7021 ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac
     7081 aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag
     7141 caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt
     7201 gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag
     7261 ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga
     7321 tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct
     7381 tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga
     7441 cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt
     7501 tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat
     7561 tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca
     7621 atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg acttacttca
     7681 aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata
     7741 aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt
     7801 tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg
     7861 tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat
     7921 agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg
     7981 ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg
     8041 aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat
     8101 catagctttg atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa
     8161 tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa
     8221 taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg
     8281 atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag
     8341 tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct
     8401 ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata
     8461 gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga
     8521 agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg
     8581 gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac
     8641 gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc
     8701 aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc
     8761 taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg
     8821 atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa
     8881 gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa
     8941 ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg
     9001 gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg
     9061 atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga
     9121 agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt
     9181 caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag
     9241 tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc
     9301 ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt
     9361 ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt
     9421 atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag
     9481 gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc
     9541 agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc
     9601 aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg
     9661 gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt
     9721 atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag
     9781 ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc
     9841 gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc
     9901 gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt
     9961 atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa
    10021 atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat
    10081 taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg
    10141 tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag
    10201 ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt
    10261 gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc
    10321 gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt
    10381 tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt
    10441 gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg
    10501 tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt
    10561 tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga
    10621 tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac
    10681 atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat
    10741 tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt
    10801 cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa
    10861 agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat
    10921 tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa
    10981 atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac
    11041 cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct
    11101 tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg
    11161 agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg
    11221 atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt
    11281 ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt
    11341 cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta
    11401 agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag
    11461 tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg
    11521 gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt
    11581 tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca
    11641 agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc
    11701 tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat
    11761 cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg
    11821 gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt
    11881 ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc
    11941 aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt
    12001 gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa
    12061 attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga
    12121 ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag
    12181 cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac
    12241 cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt
    12301 tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt
    12361 cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat
    12421 ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa
    12481 aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg
    12541 atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg
    12601 aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg
    12661 aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat
    12721 ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt
    12781 gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt
    12841 gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca
    12901 gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg
    12961 atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc
    13021 agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt
    13081 ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc
    13141 aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt
    13201 ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct
    13261 tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct
    13321 tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta
    13381 ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa
    13441 agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg
    13501 actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt
    13561 cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag
    13621 tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag
    13681 tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata
    13741 agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc
    13801 tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt
    13861 acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt
    13921 ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc
    13981 tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc
    14041 cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt
    14101 tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac
    14161 gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt
    14221 gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc
    14281 atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg
    14341 agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc
    14401 taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc
    14461 attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac
    14521 tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc
    14581 cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac
    14641 gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga
    14701 atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc
    14761 tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa
    14821 ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc
    14881 atcattaccg actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta
    14941 tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt
    15001 tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc
    15061 attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc
    15121 aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc
    15181 tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc
    15241 taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc
    15301 gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa
    15361 cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa
    15421 cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac
    15481 aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt
    15541 tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata
    15601 ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca
    15661 cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac
    15721 ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc
    15781 tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata
    15841 aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc
    15901 ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt
    15961 ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa
    16021 atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat
    16081 ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc
    16141 gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt
    16201 tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca
    16261 atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg
    16321 ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt
    16381 acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg tactttggat
    16441 tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa
    16501 gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa
    16561 gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt
    16621 agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga
    16681 tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg
    16741 tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct
    16801 acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt
    16861 tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg
    16921 cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta
    16981 atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg
    17041 acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta
    17101 tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat
    17161 aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg
    17221 attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt
    17281 tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg
    17341 tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat
    17401 cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa
    17461 cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg
    17521 tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg
    17581 ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag
    17641 gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg
    17701 tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt
    17761 tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt
    17821 gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat
    17881 caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg
    17941 aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg
    18001 catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga
    18061 atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc
    18121 tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg
    18181 gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt
    18241 gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata
    18301 actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta
    18361 attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat
    18421 ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt
    18481 ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg
    18541 gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa
    18601 tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta
    18661 cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa
    18721 gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg
    18781 agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc
    18841 gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg
    18901 atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc
    18961 gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc
    19021 gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat
    19081 gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac
    19141 caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac
    19201 ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca
    19261 tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa
    19321 tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata
    19381 cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa
    19441 acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac
    19501 cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat
    19561 ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat
    19621 ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg
    19681 taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa
    19741 atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg
    19801 gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat
    19861 tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca
    19921 gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct
    19981 gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac
    20041 caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg
    20101 ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat
    20161 catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca
    20221 gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt
    20281 ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta
    20341 tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca
    20401 taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga
    20461 aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat agaaagatgg
    20521 aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca
    20581 aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct
    20641 ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga
    20701 atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt
    20761 tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt
    20821 ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct
    20881 atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc
    20941 agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat
    21001 ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt
    21061 tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc
    21121 tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt
    21181 tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt
    21241 acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg
    21301 agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt
    21361 catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt
    21421 attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact
    21481 ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc
    21541 aattttattc cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt
    21601 ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta
    21661 tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg
    21721 ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg tcgaaataca
    21781 atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc
    21841 atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg
    21901 agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ttcaggacaa
    21961 atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga tttatagcga
    22021 aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc
    22081 attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga ttgaatggag
    22141 aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc agccgatagt
    22201 tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa cctttgccaa
    22261 tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat ccccaaaaag
    22321 aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat gtgagaactg
    22381 cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata aaaagcaccg
    22441 cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat gtgctgtgag
    22501 caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa
    22561 aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg atagcccttc
    22621 attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc catcttccat
    22681 tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca taatagtcgc
    22741 tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg catgatctgt
    22801 atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat ataaagcgcg
    22861 agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac tatttttaag
    22921 tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt ctttttttga
    22981 agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga
    23041 gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt tttctggaag
    23101 ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt gcaatcctcc
    23161 tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc tgacgaattc
    23221 tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat gaccagaacc
    23281 aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta acctcctatt
    23341 gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg
    23401 acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc aaaaggcatc
    23461 gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc
    23521 accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt tttgatccct
    23581 tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta catgaaaata
    23641 ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa ggtcatattt
    23701 tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa ctgtagaccc
    23761 ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc attgatcggc
    23821 tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca cgtatctagg
    23881 gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc gacaatcgtc
    23941 aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc tgaatcagcc
    24001 agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt cgtgagtttt
    24061 ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt gttttttcct
    24121 ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat gtatttcaga
    24181 tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga caaaacaatc
    24241 tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc attcgctagt
    24301 cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat caagccatat
    24361 cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc ctccctatga
    24421 tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt gacaatttgt
    24481 cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga taaaacctta
    24541 acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata aactaaagtg
    24601 accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg ataaagtatt
    24661 atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag tttatgatgg
    24721 tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct tggatttgat
    24781 gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa aaatacagac
    24841 tccagtattg attttgactg caaaagatag tttggaagat aagattgaag gatttgaacg
    24901 aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa
    24961 agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt ataaggatat
    25021 cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc ccatacaagg
    25081 aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt tgacaaaaga
    25141 gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag ttattgaagt
    25201 ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag aaatcaaaac
    25261 cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga gatattacac
    25321 gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt atcttttttc
    25381 ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa acagatctgt
    25441 cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa agatatcagc
    25501 aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac atggaacagc
    25561 cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg tctaaaaatg
    25621 gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa
    25681 tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac agtggctcat
    25741 ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat ggcgttgcct
    25801 acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact tttcgcacga
    25861 ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt tattacttgt
    25921 ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa tttgttgaga
    25981 atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg gaacatcttt
    26041 ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa gcattgtcag
    26101 agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg aatgattctg
    26161 atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa caacttgtta
    26221 aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat aacgatgcaa
    26281 cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt ttgctggata
    26341 atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct gttaaccata
    26401 aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa gatcaagcaa
    26461 ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact ggcggatacg
    26521 ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag atttctgtat
    26581 ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag cctaaaaaat
    26641 aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg
    26701 aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct ttaagctgac
    26761 cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt cttcaatgtg
    26821 tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg tcactttttc
    26881 aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg tatgtttgct
    26941 tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca atttttcgtc
    27001 tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt cttcacgttt
    27061 gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc
    27121 agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa ttgtatcatc
    27181 atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg tatcagtctc
    27241 gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg ttgtattttg
    27301 aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt ctaatccagt
    27361 ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt ctgttgtttc
    27421 tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat ctcctctata
    27481 ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa
    27541 agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc gattttcatc
    27601 tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg cttcatcagt
    27661 agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt caattactga
    27721 taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct cttccatgta
    27781 aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat ttattctata
    27841 ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa tttaattgaa
    27901 atttgaaaac atttatgtta atgaa
//
LOCUS       DASJRQ010000029        27725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJRQ010000029 DASJRQ010000000
VERSION     DASJRQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.029"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..2121)
                     /locus_tag="U4A80_001138"
     CDS             complement(229..2121)
                     /locus_tag="U4A80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974770.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="U4A80_001139"
     CDS             complement(2118..3845)
                     /locus_tag="U4A80_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974771.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="U4A80_001140"
     CDS             complement(3861..4511)
                     /locus_tag="U4A80_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1974772.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="U4A80_001141"
     CDS             4715..5011
                     /locus_tag="U4A80_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1974773.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5183..5803
                     /locus_tag="U4A80_001142"
     CDS             5183..5803
                     /locus_tag="U4A80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1974774.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="U4A80_001143"
     CDS             complement(5895..7061)
                     /locus_tag="U4A80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1974775.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="U4A80_001144"
     CDS             complement(7073..7426)
                     /locus_tag="U4A80_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1974776.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="U4A80_001145"
     CDS             7554..8576
                     /locus_tag="U4A80_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1974777.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8595..8756
                     /locus_tag="U4A80_001146"
     CDS             8595..8756
                     /locus_tag="U4A80_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974778.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9015..9449)
                     /locus_tag="U4A80_001147"
     CDS             complement(9015..9449)
                     /locus_tag="U4A80_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974779.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="U4A80_001148"
     CDS             complement(9559..11418)
                     /locus_tag="U4A80_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1974780.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="U4A80_001149"
     CDS             complement(11536..12696)
                     /locus_tag="U4A80_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN1974781.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="U4A80_001150"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="U4A80_001150"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1974782.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="U4A80_001151"
     CDS             complement(14443..15141)
                     /locus_tag="U4A80_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1974783.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="U4A80_001152"
     CDS             complement(15098..16759)
                     /locus_tag="U4A80_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1974784.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="U4A80_001153"
     CDS             16977..18377
                     /locus_tag="U4A80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1974785.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="U4A80_001154"
     CDS             complement(18629..19708)
                     /locus_tag="U4A80_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1974786.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="U4A80_001155"
     CDS             complement(19794..20753)
                     /locus_tag="U4A80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1974787.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22276)
                     /locus_tag="U4A80_001156"
     CDS             complement(20765..22276)
                     /locus_tag="U4A80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1974788.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="U4A80_001157"
     CDS             complement(22298..22861)
                     /locus_tag="U4A80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1974789.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="U4A80_001158"
     CDS             complement(22867..23730)
                     /locus_tag="U4A80_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1974790.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="U4A80_001159"
     CDS             complement(23744..24640)
                     /locus_tag="U4A80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1974791.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26104)
                     /locus_tag="U4A80_001160"
     CDS             complement(24815..26104)
                     /locus_tag="U4A80_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1974792.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="U4A80_001161"
     CDS             complement(26076..27593)
                     /locus_tag="U4A80_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1974793.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8107 a         5845 c         4786 g         8987 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    27721 aataa
//
LOCUS       DASJRQ010000030        27186 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJRQ010000030 DASJRQ010000000
VERSION     DASJRQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.030"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..1628)
                     /locus_tag="U4A80_001162"
     CDS             complement(60..1628)
                     /locus_tag="U4A80_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1974794.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1632..2042)
                     /locus_tag="U4A80_001163"
     CDS             complement(1632..2042)
                     /locus_tag="U4A80_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1974795.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2039..2962)
                     /locus_tag="U4A80_001164"
     CDS             complement(2039..2962)
                     /locus_tag="U4A80_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1974796.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2959..3837)
                     /locus_tag="U4A80_001165"
     CDS             complement(2959..3837)
                     /locus_tag="U4A80_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1974797.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3824..5161)
                     /locus_tag="U4A80_001166"
     CDS             complement(3824..5161)
                     /locus_tag="U4A80_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1974798.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5164..6267)
                     /locus_tag="U4A80_001167"
     CDS             complement(5164..6267)
                     /locus_tag="U4A80_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1974799.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6341..7849)
                     /locus_tag="U4A80_001168"
     CDS             complement(6341..7849)
                     /locus_tag="U4A80_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1974800.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7815..8507)
                     /locus_tag="U4A80_001169"
     CDS             complement(7815..8507)
                     /locus_tag="U4A80_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1974801.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8529..9503)
                     /locus_tag="U4A80_001170"
     CDS             complement(8529..9503)
                     /locus_tag="U4A80_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1974802.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9803..10675
                     /locus_tag="U4A80_001171"
     CDS             9803..10675
                     /locus_tag="U4A80_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1974803.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11133..13331)
                     /locus_tag="U4A80_001172"
     CDS             complement(11133..13331)
                     /locus_tag="U4A80_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974804.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13563..14555
                     /locus_tag="U4A80_001173"
     CDS             13563..14555
                     /locus_tag="U4A80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1974805.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14615..16036
                     /locus_tag="U4A80_001174"
     CDS             14615..16036
                     /locus_tag="U4A80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1974806.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16029..17528
                     /locus_tag="U4A80_001175"
     CDS             16029..17528
                     /locus_tag="U4A80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1974807.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17567..18961
                     /gene="uxaC"
                     /locus_tag="U4A80_001176"
     CDS             17567..18961
                     /gene="uxaC"
                     /locus_tag="U4A80_001176"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1974808.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19120..19803
                     /locus_tag="U4A80_001177"
     CDS             19120..19803
                     /locus_tag="U4A80_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1974809.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19805..20641
                     /locus_tag="U4A80_001178"
     CDS             19805..20641
                     /locus_tag="U4A80_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1974810.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20652..22406
                     /locus_tag="U4A80_001179"
     CDS             20652..22406
                     /locus_tag="U4A80_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974811.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22682..23023)
                     /locus_tag="U4A80_001180"
     CDS             complement(22682..23023)
                     /locus_tag="U4A80_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1974812.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23091..25262)
                     /locus_tag="U4A80_001181"
     CDS             complement(23091..25262)
                     /locus_tag="U4A80_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1974813.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25431..26330
                     /locus_tag="U4A80_001182"
     CDS             25431..26330
                     /locus_tag="U4A80_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1974814.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(26401..>27186)
                     /locus_tag="U4A80_001183"
     CDS             complement(26401..>27186)
                     /locus_tag="U4A80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1974815.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8075 a         5610 c         4885 g         8616 t
ORIGIN      
        1 aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa agaattaaat
       61 tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact tcctcttttt
      121 ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc tgagagtata
      181 cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc tttcaaatga
      241 aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc tgtgagtgga
      301 atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg taggcatttt
      361 cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt taagtacagt
      421 accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt ctccgcatca
      481 aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga ttgaagagat
      541 tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca atcagcattt
      601 ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt atccataaac
      661 tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc ttccatcgga
      721 gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac ttcttgaata
      781 gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag atatgtcatg
      841 taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg caatgtagaa
      901 gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta aggaccagta
      961 atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc ttctgccgtc
     1021 atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt cgctaattct
     1081 gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc atcatattct
     1141 tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca tagttcattg
     1201 acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa gccgttacta
     1261 tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat caaataattg
     1321 tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag ataaaacaat
     1381 ccatcagcat aactgatctg tggcgcccat atacttccat ttgtaggatt tccttgcaaa
     1441 gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt tttcgactca
     1501 tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata ccgctctttc
     1561 actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt cactttgata
     1621 gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa tgccccagct
     1681 gcgaaataaa aaaatgcaaa aggaaacgca ataaactgag tgccaatcaa caatatgaat
     1741 cctgttataa aatacaaacc agtggcagtt gttaagtatt ttcctttagc agtcaaaaac
     1801 aatgctactg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag accagtcatg
     1861 tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac agcaagcgac
     1921 tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc taacccgcca
     1981 aagaaaatca aagatagact gccaccgacc attcctagca atagttccca ttttcttttc
     2041 atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc gatgtttcat
     2101 aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca tcccaattat
     2161 cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag acatttgcat
     2221 acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca gtcaaggtac
     2281 agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct gtgatgtatc
     2341 caggattttt actggctaag ctatggatat gacactgatg aaaaacggca tctgccccac
     2401 caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga cgacaatact
     2461 caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa tcaggcaacg
     2521 gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa gatacgcgat
     2581 ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct gctgtatttt
     2641 cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta gcacttcgga
     2701 aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta ccggagataa
     2761 ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata tattcatcat
     2821 atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct gccaaataat
     2881 cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc tttccaatta
     2941 tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa tatttctcat
     3001 ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt ctggactacc
     3061 cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt cgtccaagca
     3121 catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt tttcttggat
     3181 tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc catgatgatc
     3241 taggcggcga tgtagcaaaa gtatttcgat gtccacctgt gccgctagat caatacaaac
     3301 tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca tagaaagacc
     3361 aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat cgatcaagct
     3421 gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa attgtttgaa
     3481 caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa tcgaccaaac
     3541 agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt aaggagcacc
     3601 atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca ggtaaaggac
     3661 cggcggaaac ggtccttctt tttctggata agcatagagg gctggtactg tttccaattc
     3721 atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc ggtataatcg
     3781 ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca actgcataag
     3841 caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat tggaatttct
     3901 caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga ttttcaatcg
     3961 acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt ctgctcattt
     4021 cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc atcgagacat
     4081 cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa ataaaacctg
     4141 cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg aatgctggtg
     4201 tccgttcatc tatcggataa gccttttttt cccatacata cggttctttc cctcgcggac
     4261 cacagaaata ataaagattc agtataaatg gacacatcac attatccatg actatattgg
     4321 atactcgaat atcttcaacg atcccgcctc gacctcgtct agatttcaat cgtattcctc
     4381 gatcagtttc ttggaagata caatttgaaa tggtaatatt acgaatacta ccactcattt
     4441 cacttcctaa tacgacaccg ccatgtccat ggaccatcgt gcaatttgtg atcgtgatat
     4501 tttcgcaagc gatcctttcg tacgtatctt cagtccctgc cttgattgca atacagtcat
     4561 ctcccacgtc aatatggcaa ttactaatac gcacattttt gcatgattct ggatcaattc
     4621 cgtctgtatt tggtgaatcc gctgggttca agatcgtcaa gttatcaaac gttgcattac
     4681 tgcaaagaat cgggttgatc gtccaacttg gtgactggat caatttaata tctttcacag
     4741 taattcgatg acagttatgg aaactcatca atttaggacg aggataagcg agattatctg
     4801 gttcatttct aaaagtatgc caccattttt ttccatttcc atctaatgta ccgaaaccag
     4861 tgacagagat gttctctaca ttttgagcat agatacagga agcataaact tttcgatgga
     4921 ctccttccca acgtgaatga acaacaggat aatcttttgg atcgtcagag aatttcagaa
     4981 ccgctcctgc gcttaaatga agttcgacat tgctttttaa aaataaagct cctgttagaa
     5041 attcacctgc tggaacaact acggtcccgc ctccgtcact tgctgcagca tcaatggctt
     5101 gttgtattgc ttcagtattc aattcatcaa ttgatgcgcc aaatgtcaaa atatcataca
     5161 tatttataat gcctccactt caatcaatgc ttgcaagaat gcaccaacgc ctttttgatc
     5221 attacaaata attggttcac tgatgtagta agcatatgaa ccatctcgct gatctgctcc
     5281 gcctaaccct gctacttgac agtttttatt caagttcagc cagccttctt ttgtttccag
     5341 aacaaattca tcaagtaatc cttgatgtga tttcttcact tgtggaagcc attcttcttt
     5401 gttcagcagt cccaaacgta ttcctttagc cattgcgtaa acaaacatac tactgccgga
     5461 agcttccaga taatttcctt tccgttttgt ttctgttgtt acttgatacc atgtcccgct
     5521 gttttcttct tgatatggaa gtaatgcagc cagtgtcttt tctagaattt ctttcagcaa
     5581 agaacaatct gtttcttcat gaagtaattc ataggtatct gctgcagcca tcaaatacca
     5641 gccaatcgat cggctccaaa aatgatggga tagacctgtt tttggatcag cccatggttg
     5701 gaccttcttt tcatcccatg catgaatcag caaatttgtt tttggatcga atagatgttt
     5761 ataactgatt tcaaactgtc tcaaaacatc ttggtaatct ttttcttcac taaaattctg
     5821 gataaattca gcataaaaag gagaacccat atataagcca tccaaccaaa tttgataagg
     5881 ataaatttct ttatgccaaa aagctccttc ggatgtgcgt ggatggtttg ccaactgctg
     5941 ggaaaggagt tctgctgctt ttttatattt ttcttcgcct gtttctttgt acaataaaaa
     6001 gaaaagcttt cctgtattta catggtcgat attgtattct tcaacgctgt agccacggat
     6061 cgttccatct tcttgcacga aatagtcgat attgtctttt atgaaatcaa aataaatttt
     6121 ttcttgggtt cgttcccaaa gtaattggaa ccctttaagt acgacgccat agtcataaga
     6181 ccattttcct ttataccact tttcttcata taagcgaggt gttctttcca tgatactttt
     6241 tgctaatttc tttgaccagt tttccattat ttgctctcct ttaaaaatta aaataggatt
     6301 tgggacaaat accaagaagt atttgtccca atcgtctata ttactgtgcc gctgctttgt
     6361 atgcttcctc gtactcttgt gtgattcgat cgcctccttg tgctttccag tcttctacag
     6421 cttttttgaa tccatcttcg tcaagctgtc ccatgatgta ttgcaccgta gcatcaatga
     6481 cgattttttc aatttctgat cctacttcat tgttcgtcgg tgaatccaat ggcacagaag
     6541 gatccattac agcaaattct tcattttctt tgatcaattg attagagaat tctttttcag
     6601 ggtctgtatc tttgaaagag aacgtcactt cactaggacg agagcttgat aacggttgca
     6661 cctcttgttg ccaaagatcc atatcgataa attcgataga accatcatca ttcaattcat
     6721 aatgcgttcc ttcgataccg ccagtcatca atttatacac atcttcttct aaaagatcat
     6781 caacaaattg aagtaatttc ttcaattgtg cttcgtcttt tacttgagat ttgggaaatg
     6841 ctagcaagcc gccaatacca tttccttctg accaaacatg ataaccgccg ccttcaccat
     6901 ctgagatctt gtttacaggc actaattcca tatcatcttg aataccttga cccatcgtct
     6961 tcagattctt gatatccacc attccagtgt agattccagt tcttccttgt gcaaattgtt
     7021 cttgctgatc agtcttttgg gtaactgcaa agtcttgtgc caaatagcca ttctcaaata
     7081 gatctcttga aaaattcaaa gctttcatat actcttctgt atcaaattct ggagtgaatt
     7141 tgccattatc gtctacagcc cagccatttg gtgctccaaa ataagaagtg aaaagtttaa
     7201 agctggtata acgaatatca ctaggcgcac gatcgccaaa accaactgtg tcgtccacac
     7261 catttccatc aggatcgttt tctgtaaatt gtttagctac ttcataaagc tcatccatgg
     7321 tagttggaac atctagtcct agattatcca accagtcttt tcgaatgact aagccagcac
     7381 gagcgtagtc tttttgtatc ggtacgccgt acaaggcacc gtcgatactc gcagcttcga
     7441 ttctatcttc agaaatttga cttaaatttt tatagtcttt taaatattct gatacatccc
     7501 agaatacgcc tgattttaac gcattgcgga cagaagagtt tttcaacatc gcaatttgaa
     7561 gtgtaacgat atctcctagt tcatcagaag ccaatgcagt tgttaatctt tcttcttttg
     7621 aagcatcagg aatccagttg aatgtcagtt ccgtatcggt tttttcttcg atcaagttca
     7681 caatcgtgtc tgtaggcggt gaagcatgat gcaaaatatt taaccaagtg attttagctt
     7741 ttccatcttc tgtctctgaa gaactgttac ttgaactgct gccgcatgcg gcaagtgcta
     7801 gaattcctgc aaaactaaga cctaatttta cgagcgttgc ttttttcata aattttctcc
     7861 ttcactgttt taataatatt tttgttttct tctgtttttt ctgtatatga gttcttccat
     7921 tccaaatggc aaaatataga atttgctgtc cacgtgactg cggaaaagaa gagactgcca
     7981 ccgataaagg ttaaaacacc tggcattgtt tggatgatca tcagataaat ccctaccata
     8041 cttaatatca acactaatga gtaatgaaga tgtgagaatc cgaataaaaa tgccattttc
     8101 attttgaaaa aatatccttt ccaatcataa tgagccatga ttgggaaaat atagagtgct
     8161 gagagcacat atatgaacgc agcaatatac aaagcgatcc taagatacca attgtgtatg
     8221 aaagaaagat ccacaagtaa aacaatcccg atggcgccat atatccagct catcagcaag
     8281 gtttcacgat agttttcttt aaaatatgtg aagtacgttt tcgtcaaagg aaacgtttct
     8341 tttgagcgca gccattttct aatcactgca accatcgcat acgatgcagg accgaaagta
     8401 aaaactccca aacctgcgat gactaaaccg ccccacagaa aattcaaata aaccaactgt
     8461 aaaatacgat ttagccttcc gtttatttta tctaatgact gcagcatatc tttccctctc
     8521 tttctatctt agtaaacgcc gtcttctcct agctttttgg caatcttatt agctaaaatt
     8581 accataaaca atcctacgac ccctttaaac aatcccattg ctgtactgaa acttaactgt
     8641 ccattcttca atccagctgt ataaatatag gtgtcgaaaa tttctgcaac gcttcgattc
     8701 aatgaattca ataacaagta catatgctcg aaccctaagt ctaatgtctg accgattttt
     8761 agaatcagca aggtaatgat tactggacga attgccggca atgtaacatg ccatgttttg
     8821 cgtaagcgtc ctgctccatc catttctgca gcttcataca actgtgtatc gactgcagtt
     8881 atcgctgaaa ggtagatgat cgtagaccag cccaactctt tccagatcac ttgtccgata
     8941 tatactgtgc gtgtccattc aggggatgtt agaaaactga ttttttcaaa tcccattgtt
     9001 gcaaggaatt cattcaatac ccctccgtca acattcaaaa atacataagt aatcgaaaca
     9061 atgattaccc aagacatgaa gtgagggatg taaatgatcg tttgaatccc gtttttcaat
     9121 cgtttgttcg ttacttcatt taatagtaaa gacaaaatga ttggcatcgg aaaaaacacg
     9181 aagatattta atccgaacaa gatcaacgta tttcttaata atgtaaagaa tgtcggttct
     9241 gtaaacaaac gaatgaaatg tttgaaaccg acccaaggac ttcccattat tcctaaaaat
     9301 ggttgataat cttgaaaagc aataacgagt ccccccatag gaagatacct aaaaatcaag
     9361 aaataaagga ctcccggcag aatcatcaca tataaaaatt tattcgtttt gattcctgca
     9421 attatttttt ttcgtcttct gagcttttct tcttttttcg atagcgtcag ttgaaatggt
     9481 tcttgtttct ttacactttc cataagcccc tccttagttt tgtgaatgtt tgctgcttat
     9541 gacttcatga tagataacta tctgaattac gtatataatc atttttaccg taagtaaaaa
     9601 cgcttacata gcaatgattt ttgtgctata caaaacgata aagtgtattt tttcacaaat
     9661 aaactacgca tagcaaacct tctatttttt tctatctgac acttagcata ggaaatgatt
     9721 atatacgtaa tctttcaccg atgataaatt gaacatatag aaacagaaac atttaattta
     9781 ttttgaggag gagcctatct atatgaaaaa gaaagtctct ataggagaaa gaagttttac
     9841 acttttcaac aatacgtttc taattttgct agctttgatc tgtatcgttc cttttttaaa
     9901 tatcatcgct acatcttttg cttctacaca agaagtcgtc gcgaaaaagt ttatcctttt
     9961 tcctactacc ttttctttag acgcgtatcg ctacattctt tctacgccga ccatttttag
    10021 agcacttgct gtttcgattg gtgttacagg tgtcggaacg atcgtcagta tgtgtgcaac
    10081 ttcactgatg gcctacggtc tttccagaaa gtatttgttt ggtcgaggtt tcgtcaactt
    10141 tcttgttgta ttttctatgt tgttcagcgg ggggatgatt ccaacgttct tagttgttcg
    10201 ttctctaggg ctggtcaatt cttactgggc catgatcctg cctgtggctg ttaatgcaat
    10261 gaatatgatc attatgagaa actttttcca agccttgccg gatagtttgg aagaatcagc
    10321 aaaaatggat ggttgcacag attttggtgt atttttcaaa attatgctgc cattagcgtt
    10381 accgtctatt gctactatct cgctttttta tgctgttact tactggaaca cttatatgac
    10441 tgctattttg tatatcaacg attcatctaa atggccgata cagattcttt tacgccaaat
    10501 cgtcattgta tcaagtggga tgcaagcaga aagtagtgcc gttgatgtga ttccaccagc
    10561 acaaacagtt aaaatggctg ttatcgttat tgcaacagtg cctatgctga ttgtttatcc
    10621 ttttgttcag aaatattttg tcaaaggagc actcgtaggt tctgtcaaag ggtgattatg
    10681 tactgaacgc aaaatggtag ttgtgtcaga agtggtcagc ttcaagaaat aagaaggcat
    10741 ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttttcga agaatctact
    10801 cctggaacac cgtgtatcag aggttgtgaa agagacgttc agccttgagc gatgataaaa
    10861 actctcacaa acattgcgac gagtaaccca caggagcaat aagaagcgat tttgaaaaat
    10921 agcttcccat atttcacaaa attatgcctt attgtcgata gcctgtctct tgaacgccat
    10981 ttattcgtaa aaaagaactg tgacaagact tttgtcacag ttctttttgc gtgcttagcc
    11041 ttgaaattta actatccata aatacaaata ctagggttat tctaacttct tttattgctt
    11101 gaatcagtcc acaagaaaaa cagcctcttg attcacaaat atttttttcg atattctccc
    11161 ggcgtaatcg attccttctt tttaaagaaa cgaataaaat tttgcggatt tcgatattgc
    11221 aatctttctg caatctcttt tacggagagg ttagtctctt tcaaccattt ttttgctacc
    11281 tctaaacgat gattttgaac aaaatcacca aatttttctc ctgtttcctt tttaaataca
    11341 ttacttaaat agttagggtt ataatgcaac ctatcagcga tcacatctaa agacagatca
    11401 cgatcatatt ctgtgtagac gatatgcata atattctccg acaaattttt aaattcatga
    11461 tcagtgattg cttgagagca ttctgtgatt ggcagaacta attgttctaa aacaagtttt
    11521 tctagttctt tgggatgata attgttcagt gcttttacat aaatttgttt catgttatca
    11581 aagttttttg aatctactcc caataactgt cctaattgaa cgagttcatt gatcaagcgg
    11641 accgaaacta cttcccgact caaaggattt ttattttgtg caaacaatgc atcaatcaat
    11701 tcatgtacta aattcttcac ttcctgattt ccagaacgaa tggcttcaaa caatcgattt
    11761 tgttcttcta tcggatatct aataagtgtt ttttcatgag cagcaggaac gatttcctga
    11821 taaaaaataa ttgaacttgg accagtattt acacgataat acagggcttc ttttgcccga
    11881 tcaaccgatt gcttgctttc attcagcgtt tcgaaccggt tactgatgcc tatactgata
    11941 gttaaattca agtattcttt tacacgtttt tgaatgagtc ggcaatactc catgactttt
    12001 ttgcttgctt ctgcatcatc tttatccatt cgataaatag tggcttgcat ttcttcattc
    12061 aaaacgatcg gcagcattcg attttccttt ggcacaattt ccgtgatcat gttgttgatc
    12121 gctaaaagta gcaaatcacg ctccccaccg tgactatctt ctaacagatc gatttgaacc
    12181 aaagctgtat aatagcagtc attttgagaa ctataaccaa attgctgcaa ccgctgatct
    12241 agttctcgcc tttctactcg atttctaaat aaactaagta caaatagcgt ttccagctgt
    12301 ggcttttggg taactaagtg tgcacttaac gcttcttttt ctcctatgat ccgactgact
    12361 gattgagcga ccaaatctag ttctttccca ctgaatcctt ttgatttaag attcagacgt
    12421 tcctgtattt gagaaatcgg acgggcaaat ctgtccgaaa ataagtagga aagaagacca
    12481 atgagtccta ccagtgttaa agacactgca actagcccga ttctcagatt gcggatcatt
    12541 gcgccaattg cactagagtc aacttctaat ctgtaaatcc aattattata atctgatttc
    12601 agtactatat acgttttatc tttttctctt atcactttca gctgattgta atctagatcc
    12661 acgtccgtta atacagttgg ttgctcttta ttttttactg tctgagtagt aaaaaggttg
    12721 ccaacttcgt tttcaataga aagtatgtct tctccacttt caccaacaac ttgttctatt
    12781 gtatgtttct ctatcgtagc aattcccaat gcatattttt ctttttcaaa cattggcaaa
    12841 gaaatcacca tttcgattcc tttgtttttc ttttgccaaa ataaattatt tttgttattt
    12901 atgtacttct gttgatactt ttcaatttcc tcttcggtca gttgactcaa cgatccattg
    12961 attactcccc atttaccagc caaactgact aaatcataat tattatcatc cataacaatc
    13021 gtttctataa aatttagttc tttcctgatt tcagaataag tggagtaatc tgtatatgtc
    13081 agcggatgct tgaatatgtc cgagaaattt ttggtcgaac catacgtaga aaacgaatat
    13141 tcaatcgttc taattttcca ttcaacgtta gttttaactt gcctgagcaa attctttttg
    13201 tctgttaaat aactcgattc gatcgattca taggtattct ggtaaatgaa taaactaaat
    13261 cctatgacaa gtacggctcc taataaaaac atcggtaaaa atattttata aaatactgta
    13321 cttttcttca ttttcttcct ctcctccttt taaaataaaa ggctttcatt tttctgtaaa
    13381 attgatactc atctaattaa atgagcattc cccgctttca agaatagcat aattaaaaat
    13441 aaaaatccaa gtaattttta acgtgtgcaa tcttttcata tcaaagatct attgacttat
    13501 cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg
    13561 gaatggtaac gatcaaagat gtcgctaaag cagctggcgt ttctcatacg acagtttctc
    13621 gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa acaaaaaata cgacaactag
    13681 ctaaggaaat gaattattcg ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat
    13741 atctaatcgg actatttttc tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag
    13801 attccattgc aggcatccat tcagtattag acacaaatta cagtttatct gttgaaagta
    13861 tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta cgatggactg atcgttatga
    13921 gtcaaagtga tgatgatcag cctttcatcg actatctcaa agaaaaagaa tttcctttcg
    13981 ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa cgtcactgcc gatgatgcgg
    14041 ccggtgttat gcaggcagta gattacggta ttggatgcgg acatacgaag atcgcttata
    14101 ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc
    14161 taaaaaaagc gagcatccct gtcaatccag cctatttttt aacaggagat tacagcttag
    14221 aaagcggcct ccaaaacatg gaatacctat tatcactttc tgacctgcct actctggttt
    14281 tttgcggcaa cgacgacata gcgattggtg cattgagagc tgtacattta gctggcctcc
    14341 gtgttcccca gaacatttct ctgatcgggt tcgatgattc tccagtcgtc tcctatctca
    14401 atccgccgct taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc
    14461 ttttactaga tttgatcgaa ggcaggacga tcgaacaatc taaaattgag ctctccacct
    14521 tgttgatcct tcgtgagtcg atcaattact tgtaaactac aagtaatttt atataaaaat
    14581 gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa aaactgacaa aagaattagt
    14641 gaaaaaaaac gaagcacctg taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg
    14701 atttattgat tggcagattc agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc
    14761 aatcattcaa cctttagaac aaggtcttgg aaatatgatg aaccagcaag ataatttata
    14821 tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg agattatcac
    14881 cgtagtagaa caaatgatca atccttatga tgaatgggat gcttatctat ctctagcaga
    14941 aaatgacgac ttggcctata tcgtctccaa tacaacagag gcaggaatcc aattcgtgga
    15001 gactgataat ttaacagata ctcccccaca gagcctcccg ggcaaactga cagccttttt
    15061 ataccgacgt ttcaaattaa acaaagctgg ctttaccatc attccttgcg aactgattga
    15121 cagaaacggc gaacgtctaa aagaaatcat tttacgctac gcagaaaaat gggatttaga
    15181 acctgctttt gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg
    15241 catcgtccca ggttatcctc gcgaagatgc aggtgttttc aatcaacggc ataactatga
    15301 agatcaattg atggtcaaag cagaaccatt catgctttgg gtgatcgaag ggcctaaaga
    15361 attggagaat caactaccat tcaaacaggc tggcttgaat gtcatcatca cagatgatat
    15421 gactccttat cgtgaacgta aggtgcattt gcttaatgga ccgcatacag cgatggtccc
    15481 tcttgctttg ttggcggaat tggaaactgt agaagaagta atgaaagatc ctttattctc
    15541 cgcatatgtt gatcagttat tcaatctaga attgattcct atgctttctt tacctaaaga
    15601 tgaattagcc atttatgctg accaaatcaa agaacgtttc ttgaatcctt ttgcccatca
    15661 caaattagaa gcgatttcat taaatagtgt ttcaaagttc agcacccgtc tgcttccagt
    15721 gttcaagaag tatatcgaag aacaaaatca ggtaccaccg ttaatcactg tttcactagc
    15781 tgcgctactt cttatgtatc gaggcgatca agtaaagcca catgatgatg aaaagacgat
    15841 aagcgaattt actgatgctt ggtctgataa tggaacagct attcctcgat tattgcaaaa
    15901 cgctgcgctt tggggagaag atttgtctca aataccgaat gtgacagata cagttcaaga
    15961 aatagctgca aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac
    16021 gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg
    16081 tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac gattgatcag caaacgatca
    16141 ccactcttga agatatcccg caaggtcata agattgcatt gggtgatttg aaagctggag
    16201 acaacgtgat caaatatggc tatccaatcg gtcatgtgac gacagacgta actgccggtc
    16261 aatggctgca cactcataat gtaaaaacca atctttctgg tgaattggat tatcgatatg
    16321 aaaaagatgt ccatccaagt cattaccttt ttgagaaccg aacatttcaa ggttacttgc
    16381 gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt agtacctacc gttgggtgtg
    16441 tgaatggcat tgctgaactg atcgtcaaac agttcaagga aaaacatcca gatctgggac
    16501 agtttgacca tattacgatt ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc
    16561 atcaaaatac tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc ggaggagttt
    16621 tagtatttgg attaggttgt gagaacaata cagttcctga attcaaaaaa attttaggtg
    16681 aatatgatga agaacgagtg aaatttctag tggctcaaga tgtttacgat gagatcgaac
    16741 aaggtgtggc tctattagaa gagcttttaa cagcggcgga aaatgaccag cgcatatcag
    16801 tcccgctctc taagttgaat gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa
    16861 taactgctaa tcctttactt ggtgcatttt ctgactactt gattgctcaa ggaggcagca
    16921 cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg
    16981 aaaatgaaga agtttttgac tcaatcgttg acttgatcaa tgacttcaaa cagtatttct
    17041 tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg taataaagca ggtgggatca
    17101 ctacattaga agataagtct ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg
    17161 atgtgctaca atatggcgaa aaaatcagaa aaccagggct tagtttattg caagctcccg
    17221 ggaatgatct tgtagctgcc tctgctcttg cttcatccga ttgccagttg gtcttgttta
    17281 ccacaggaag agggactccg tttggctcat atgtcccaac ggtcaaggta tctacgaata
    17341 caactctttt tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc ttattagaac
    17401 aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat catcgctgta gctagtggag
    17461 aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc catttttaaa aacggagtaa
    17521 cactttgatt ttcataaaac agtataaaaa gaaagaagga agatttatgt ttttatctga
    17581 tgatttttta ttaaaagacg attgggcgaa aaagctttac cattcctacg caaaaaacat
    17641 gcccattatt gattatcact gtcacttaga tccaaaagaa atttacgaaa acaacaattt
    17701 cagcaatctg acagaagctt ggttaggagg cgatcattac aaatggcgct tgatgcgagc
    17761 ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct gattttgaaa aatttttagc
    17821 ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt tacagttggt cacatcttga
    17881 attgaagcgt ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg
    17941 gacacaagca aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa
    18001 atcgaatgta gaagtgattt gtacaacaga tgatcccgca gacgacttgc actatcatca
    18061 gttgcttgct catgaagaac cagatttgaa ggttctacct tcattccgtc cagataaagc
    18121 actcaatatt gaaaaagaag gatttaatgc atgggtcaca ctactggaag aagcagctga
    18181 ccaaacgatc accacctatc aagaactgat agaagtctta gggcagcgga tagaatactt
    18241 ccatcaacat ggatgtcgtg tttccgacca cgctttagac atcttacgct ataaagaagc
    18301 ggatgaaaca gtattagaag agatttttca aaaagcacga gctaacaaac tattatcttc
    18361 agatgaaatc gatgcttatc gtacagaaac actgatacat ttgacccact tctatcattc
    18421 tcataattgg acgatgcagc tgcacatcca tgcttatcgt aactgtaata cacaaatgtt
    18481 ggaaaaatta gggcccgata ccggctatga tggaatgaat gatctttctt tgactattcc
    18541 tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag ttgccaaaga cgatccttta
    18601 ttcattgaat ccgaatgact atcctgcgat tttagctttg atgggctgtt tccaaaaaga
    18661 acagaatgga aaactgcagt taggttctgg atggtggtac aacgatactc gagctggtat
    18721 gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg gggaattttg tcggcatgct
    18781 gacagattca agaagtttcc tgtcctacac tagacatgaa tatttccgtc gtgttctttg
    18841 tgaattttta ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac aacttttagg
    18901 aagtctagtt caagcgatta gttatacaaa tgctaaaaat tactttggct tttttgccta
    18961 attgattaat agtaacagta acgaatcaaa aacaggatag tgaaataacg gaatccgttt
    19021 atccacgttt tagagaagaa gacggaccaa atttgctccg tcttcttctc ttgctgttct
    19081 gcttcattgc ctataaacct ttatgaaaga agagataaca tgcctactat ccatattgcc
    19141 ggtgattcta ctgcggcaga aaaagatgaa aacaagcgtc ccgagacagg ttggggagaa
    19201 aaaatctctg cttatttttc acccgagatc cagatcaata accaagcaaa gaatggacgc
    19261 agcaccaaat catttatcca ggaaggaagg ttagcccgtc tagcttcaca gtttcagccg
    19321 aatgatttct tatttgtaca atttggacat aacgaccaga aaatccaaga agaaagaggt
    19381 acgcttccct tcggtagcta tttaaaaaat ctgcagatat tttttgaatg cgcaaaaaaa
    19441 gcgaacgtcc aaatggtctt actgacatct gtgacgcgac gagattactt agaaaacgga
    19501 acattgaatc cagatatact tggagactat ccaaaagcga tgcgtgcatt tgctgagaaa
    19561 caccatattc ctcttttaga tgtgttctca agatcacaag aattgttcca gaccttctca
    19621 aaagaagaga caaaaaaatt ctacctgcat cttatacctg atacttgtaa aaattaccca
    19681 gaaggactaa aagacaatac ccactttagt ccagaaggcg ctgataaagt tgcccgaata
    19741 attgttgaat tgataaaaga atcccgtctg cctcttgcta attatttaca aaaaggagtt
    19801 tgagatgttt ccattaaatt taggcatccg tgcccacgat ttagatgttc gaaatcgtga
    19861 agaaatcgtt caaaaagtat cacagcttca cttgtcccat atccaatttg caccacaaaa
    19921 agcatttcct gaacaactta aattaactga catgacactt gggagtgctg cctattttgg
    19981 tgaatacttc caaaaaaaca atatcgaact ttccatttta ggatgctata tcaacctatc
    20041 cagcaaaaat caagaagttc gtgcacaagc attggcaact ttcaaacagc aaattgctct
    20101 gtgctctgcc tatcaagcca agatgattgc tacggaaacc ggtagtgtct ctgtcggtta
    20161 cacaaaagaa aattttactg aagaagctta tcagatcgcc agaaattcta tcattgaatt
    20221 agtcgctcat gcagaaaatt ttggcgtaac tgttgctgtt gaagcaggga tcaatcatcc
    20281 aatctataac tatcagttag ctaaacgttt aatcgatgaa gtagcttcac caaatttaaa
    20341 aatcatttta gattgtgcga atttgatgca taaagaaaat catgctgatc aggaaaaagt
    20401 gatccgaggt gccttggaca atttacatgg ttatattaca gcgcttcatc tcaaagacta
    20461 tatcatggaa aatggaaaga tccgcatggt acctgttggc aagggctgtt tagatttccg
    20521 cccggtactg cattatatca aatatgagcg tcctctaatg tacgccactc ttgaagctac
    20581 tcctgagatt tatgttcctg aagcaataga acacttgcag tttatttacc aagattgtta
    20641 aggagaagat tatggaggaa cgaatacaga aaagttatca aaaatatcat gcaaatgctt
    20701 ttcagacatt gctgcatctt tatcagggaa attattggcg ttttgccatg tcaggactct
    20761 tttttattat caaacatctt ccatcttggt taatgccgat cgctattgcc aatgtaatca
    20821 atgcagcttc atcgaaagat tcttctcagt tgcaggccat ttatttcaac gcttttttga
    20881 tggcttgtct gattggtcaa aatatattga cgaattattt tcatgtaaaa taccatagca
    20941 cttcgatccg tcaagtagaa gctggactaa gagcgaccct tatcaagaaa atccaagaac
    21001 tatctattcc ttatcaaaaa gaattgcaat ctggacgaat ccagtcaaag atcatccgtg
    21061 atgtagaagc tatccaaacg ttgtcctctc aagtatttgt cagcggatta aatatcgctg
    21121 tcaatttatt cgtagcttta ggtatcacgc tttataaaag tcctgttgtt ttcatgtttt
    21181 tcttattgac tgttcctctc gccgcattcg ttactctctt tttccgtaaa aggatcagac
    21241 agtccaactc agacttcagg caagaaatgg aagagacttc tgcaaaagtg atcgaagtga
    21301 tcgaattagc accgattgct cgagctcatg ggttagaaaa acacgaaaca actcgcttaa
    21361 ataagcattt tactcaaatc tatgaaaaag gattccactt agatatcctg caatctgttt
    21421 tcggatcagt cagctgggct agttttcaga tatttcagct gatttgtttg attttcactg
    21481 ggatattagc tttacaaggg cgcatccaac ccggcgatgt cgtgatgtat cagacgtatt
    21541 tcacgacgat cgtcaattcc gtttctggaa tgatcacgct gattcctacg atttcaaaag
    21601 gcatggaatc tatcacttct attggcgaac tgttgattgc tggcgatgta gaaacttatc
    21661 acggtaagaa aaaaatccaa catttggatg gaaattttcg ttttgaggat gtttcttttc
    21721 tttatccgga ctcactcaaa ccgattatcg atcatttttc tctagaggtg aaagctggag
    21781 aaacaattgc ttttgttggg ccatctggct ctggaaaatc tacactcctt aacttattga
    21841 ttggatttat ccagccgact tcaggaaaaa tttttctaga tgatcagcct ttggatgaac
    21901 tggatatgcg aagtgtgcga aattatttag ccgttgttcc tcaaaccact ttgttgttct
    21961 ccgcttcgat caaagagaac atcacttatg gcttgaaaaa cgtatcaaaa gaacgtttag
    22021 atgaagtcat cgaagctgcg caactttctt cactgattga ccagcttcct gatggtctgg
    22081 ataccttagt aggcgaacat ggcaacaagc tgtctggtgg ccaaaagcaa cgtatctcca
    22141 ttgcacgagc gttgatacgt cagccaaaaa tcattttgct agatgaagca acctcagctt
    22201 tagataacca gtcagagaaa aaaatccagc aagccctgaa ttacctgaca gaaacaccca
    22261 ctacttttat tgtggctcat cggctttcaa cgatcaaaga ggcagataaa atcgttgtga
    22321 tcgatgaagg aaaaatcgtt gaaataggta cttacgaaaa actgttagct caaaaaggct
    22381 atttctaccg cttgaaaaat gaatgacaaa aaataggagc tatgacaaga cttttgtcgt
    22441 agctcctatt tctgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    22501 gtcatcaaac atgagaagct gttttttgaa attgttcctt gtaactcaga gttaaacggt
    22561 aggctcacga cctcttggta aatggtgttc aagagacagc ctatcggcaa taagaagcaa
    22621 ttttgtcatc gctcaaggcc gaacgtctct ttcacaccct ctgttgtttc ataaaatagt
    22681 tttattcgct gtaaaggtcg cgaactggtg tcatgatgat acggttcaag tcattgatga
    22741 ttgtactgaa gccttgctct ttttgcatca agtcattgat tacttcttct gattggattt
    22801 tcacagccat ttcttgtgct ttcattgcat cttcgtcgct gaactcttgt ccactcattt
    22861 gtttttcttg taattcgatt tgcatcgctt ggaagtcttt gaacagtttg tgtgcttctt
    22921 cgtttgcttg tactttctcg tacgcttctt tcaacgcttt gaattctggt aattcacgga
    22981 tctcacgttc gatttgattc gcgctatcgt agatgtttga catgttatgc cctcttttct
    23041 tttagtagtt actctctatt gtaacatggg gttattcgtt ttcaaagggg ctactctcct
    23101 tgatatttcc gccagaggtc tttagccgct tcaccaattc cctgaacgcc ttctttcacc
    23161 atatcgccga agtttttcag cccttctttt gcttgttcac catattgatt caagttatct
    23221 tgccaatttc cagactctcc attagaattt cctgtgtttc caacttgatc tgcagctacc
    23281 acttgtcctc ctgtggcata ggcatctgct acactaaatc ctgtttttga agtatacgga
    23341 aggatgcctt ctgccgctga tttgaagact ttcccgacaa catttccact tgtcccttcc
    23401 aaatagtgca gcttgctgga ttcttcgtaa cccatccatg tcgaaatcac tacgtctggc
    23461 gtgtaaccga tcatccattg gtcatttgct tttgtggcat cgaaattagt ttctgtggtt
    23521 cctgttttcc ctgctatcgt ataacctgca ggattcgcag cagccgctgt tccatttgaa
    23581 aatgtaccaa gaagcatgct ggtcatttca tcggctgttt cctttgatat gacctgtttt
    23641 ggttttttgt ttgtgttatc gacgatcacc gctccagtcg aatcaacgat tttcgtgata
    23701 agatgcggcg tatacatctg accatcattg gcaaatgcac tgtaagcacc cgccatgatc
    23761 aatggagata ctcctttttc cagtccacca agggctaagc cataatactt atcagatttt
    23821 gctaaaggca ggccgaattc ttctgcttta tcatatcctt tttgtaaacc gatctcatgc
    23881 aaaagccata cagtaggcaa attcaaactt tgtgcaacag cttgatacat cggtacttct
    23941 ccttggtatg tcccgtcaaa attatgcgca tcgtaataac tttgcggttc gtctttcaag
    24001 atactggaag gcttgtatcc agcttccagt gctggtgtat atacgcttaa tggtttgatc
    24061 gttgatcctg gtgaacgacg catctgtgta gcaaagctga atcctctaaa gacatgatct
    24121 cctcttcgtc ctactagtgc ttgtacacca cctgtattcg gatctaaagc tactgaagca
    24181 gattggacca tggcaccatc tgaagcattt gctgggaata atgcactgtt ttgataagta
    24241 gcttctaatt gcttttgata atcctgatct aaagctgtat agattttata gcctttattc
    24301 atgacatctt cttcatcaag accatactcg ttaactgctt catcgatcac tgcatcaaaa
    24361 taataaggat aacgataacc agaatctgaa ttatcgtatg tgtcattaag atacgcgctg
    24421 atatctgtct ggctttctgc ttgttcttgt tcttttgtga tttttccgtt ttcttccatt
    24481 acacttagta ctgtgttttt ccgatcattc gcattttttg ggtgatcgat cggattataa
    24541 ataccaggac ctttcagcat tcctgccaaa gtagcagcct cagacagcgt aacattcgtt
    24601 gcatctactc caaaatactt tctggaagcg tcttggacac cccatacccc gttgccaaaa
    24661 taggcattgt taagatacat ggtcaagatt tcttctttgc tgtacttctt ttcgatctcg
    24721 attgccaaaa ataattcctt cgcttttcga tcaaacgttt ggtctaatgt cagatacgca
    24781 tttttggcaa gctgctgagt gatcgtactt cctccgcccc ctgatgttcc gccagacaag
    24841 accattcgga ctgctgcacg ggcaatccct ttgatatcaa acccgtgatg ctcgtaaaaa
    24901 ttcctatctt ctgtcgagat tactgcatct tggatatagg gagaaatcgc gtttaattct
    24961 acatatgttc ctttttgggc gtacagtgtg ccagcttctt cgtttttctt atcataaatg
    25021 actgtcgatt ggcttaatcc tgattttaac gtttcgacat ttgcttgttt tgctaaaata
    25081 aagaggtaga tacttgtcac taagacacag atcaaaccaa tcagcaaaat caatttgttc
    25141 acatgatatt ttttccagat gcgctttcgc catcgatgga attgtccgag atatggtctg
    25201 atccaacgcc aaaaactgat caaagcagct tttatctttt ctaaaatgct ttgaaaatcc
    25261 atttgcttct tctcctgtct atttgattca atcgtcttat tttaccatag tgaaagagtg
    25321 ttttcttcct gcttaagttg tagttcaata tgttttttta cgttagtgca agtagagtaa
    25381 cataagaatg aacagaaaaa tgtaagtttt ttcttaaata tgttttaaaa atggtacaat
    25441 tagtctgcga ttgcactgaa aggagtacac ttgtgaaaat cacgatcact ttacctatga
    25501 accatcctga aacaacaatt cgcgacttgc ttgaaaagga atggttagtt cctagaaagg
    25561 ttcgacactt tttgcgtata cgtaaaaacg tttggatcaa tcaacaacct gctttgttcc
    25621 atcatgaagt aaagggcaat gaccagatca cattgttatt cgaagaaaca gattataatt
    25681 atcaagaagt tcaattagga gatgcgaagc acatccgtgt tttatatgaa gatgagcatt
    25741 tgattatagt ggataagcct gcaggaatga agactcatcc aaatgaaccg acagaaaaca
    25801 gtaccttact caatcattta gctgcttatc tcgacactaa acagcaacga ccttacgttg
    25861 tccaccgctt ggacaaagaa acaagcggtg ccgttttatt tgcaaaaaat ccatttgtct
    25921 tgccgatact tggaagaatg ttggaacaaa aaatgatcta tcggagatat caagccatcg
    25981 tatggggaga aattgccaaa gatatgacca tcaccaataa aattggacga gatcgccatg
    26041 atcggagaaa aagggtcgta gatgataaaa atggtcagag cgccttgaca catgtcactg
    26101 tcaacaaagt actttctgaa caaacacaag tctattgtgt tttggaaacc ggacgtacac
    26161 accagatcag agttcattta gctcataagg gacatccgat cgttggagac cctctctacc
    26221 aaaatagaac agctgaacga ctgatgctcc atgcattaga actgcacatg atccatccat
    26281 ttactcaaga aaaaatagtc ggtaaagctt tacctggatt atgggaatag aaataaaaaa
    26341 gaacagagtc ttatcagcag ataatgataa gactctgttc tttttgtgta atttgtctgc
    26401 ttacttttga tgttcgtgtt ctgctaacag caaactaccg ataatcccac tttcattcgg
    26461 cagtccccaa cgaacgatat attcacttac ttgaggtgtt tccatataac cacccatcaa
    26521 ttcaacaaat tgctgacgga ttttttgaag gagatgatcc tgattcatta ccccaccgcc
    26581 taaaataatc ttttcaggtg ctaacgtcaa cgtatagttg atcagtgctt gagcaatata
    26641 ataagcctga atagcccaaa caggatgatc ttgcggcaga tcttgccctt tgatccctgt
    26701 gcgtgcctca atagacggtc ctgctgctaa tccttctaaa caatctttat gataaggaca
    26761 agtaccttca taatggtcat ctggatggcg tttcacccaa atatgtccca tttcaggatg
    26821 tgcgatccct gaaaaaattt cttcattgat cacgacacct gcaccgattc ctgttcccac
    26881 ggtaaggtaa atacaactat tttttccttg tgctgctcct tgcttcaatt ccccatatgc
    26941 agcagcattg acatccgttg tccaagcagc aggaatattg tagcgttcct tgattgtgcc
    27001 aagaaaatcg aattgtgccc accctttttt gggagttgcc aaaacgtatc cgtaattatc
    27061 ttttgttttg tcgataccaa tcgggccgaa tgagccaatc ccgattccct tcaattcata
    27121 acgatcaaaa aattcaaata cctgatccaa tgtttcttct ggtgttgtgg tagggatact
    27181 taattt
//
LOCUS       DASJRQ010000031        27029 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJRQ010000031 DASJRQ010000000
VERSION     DASJRQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.031"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(149..2302)
                     /locus_tag="U4A80_001184"
     CDS             complement(149..2302)
                     /locus_tag="U4A80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1974816.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2596..3444)
                     /locus_tag="U4A80_001185"
     CDS             complement(2596..3444)
                     /locus_tag="U4A80_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1974817.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="U4A80_001186"
     CDS             complement(3668..5608)
                     /gene="thrS"
                     /locus_tag="U4A80_001186"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1974818.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5945..6124)
                     /locus_tag="U4A80_001187"
     CDS             complement(5945..6124)
                     /locus_tag="U4A80_001187"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1974819.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="U4A80_001188"
     CDS             complement(6171..7994)
                     /gene="cadA"
                     /locus_tag="U4A80_001188"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1974820.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8272..10551
                     /locus_tag="U4A80_001189"
     CDS             8272..10551
                     /locus_tag="U4A80_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1974821.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10644..11291)
                     /locus_tag="U4A80_001190"
     CDS             complement(10644..11291)
                     /locus_tag="U4A80_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974822.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11470..12189)
                     /locus_tag="U4A80_001191"
     CDS             complement(11470..12189)
                     /locus_tag="U4A80_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1974823.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12320..13471)
                     /locus_tag="U4A80_001192"
     CDS             complement(12320..13471)
                     /locus_tag="U4A80_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1974824.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13688..14428)
                     /locus_tag="U4A80_001193"
     CDS             complement(13688..14428)
                     /locus_tag="U4A80_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1974825.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="U4A80_001194"
     CDS             complement(14432..14755)
                     /gene="rsfS"
                     /locus_tag="U4A80_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1974826.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14812..15405)
                     /gene="yqeK"
                     /locus_tag="U4A80_001195"
     CDS             complement(14812..15405)
                     /gene="yqeK"
                     /locus_tag="U4A80_001195"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1974827.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15389..16039)
                     /locus_tag="U4A80_001196"
     CDS             complement(15389..16039)
                     /locus_tag="U4A80_001196"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1974828.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="U4A80_001197"
     CDS             complement(16049..16360)
                     /gene="yhbY"
                     /locus_tag="U4A80_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1974829.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="U4A80_001198"
     CDS             complement(16376..17485)
                     /gene="yqeH"
                     /locus_tag="U4A80_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1974830.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17482..18009)
                     /locus_tag="U4A80_001199"
     CDS             complement(17482..18009)
                     /locus_tag="U4A80_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1974831.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18189..18974)
                     /locus_tag="U4A80_001200"
     CDS             complement(18189..18974)
                     /locus_tag="U4A80_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1974832.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18967..19836)
                     /locus_tag="U4A80_001201"
     CDS             complement(18967..19836)
                     /locus_tag="U4A80_001201"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1974833.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19837..21216)
                     /locus_tag="U4A80_001202"
     CDS             complement(19837..21216)
                     /locus_tag="U4A80_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1974834.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="U4A80_001203"
     CDS             complement(21224..21643)
                     /gene="fabZ"
                     /locus_tag="U4A80_001203"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1974835.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21668..22144)
                     /gene="accB"
                     /locus_tag="U4A80_001204"
     CDS             complement(21668..22144)
                     /gene="accB"
                     /locus_tag="U4A80_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1974836.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="U4A80_001205"
     CDS             complement(22147..23382)
                     /gene="fabF"
                     /locus_tag="U4A80_001205"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1974837.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="U4A80_001206"
     CDS             complement(23401..24138)
                     /gene="fabG"
                     /locus_tag="U4A80_001206"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1974838.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="U4A80_001207"
     CDS             complement(24150..25067)
                     /gene="fabD"
                     /locus_tag="U4A80_001207"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1974839.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25174..25398)
                     /locus_tag="U4A80_001208"
     CDS             complement(25174..25398)
                     /locus_tag="U4A80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1974840.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25451..26413)
                     /locus_tag="U4A80_001209"
     CDS             complement(25451..26413)
                     /locus_tag="U4A80_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1974841.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26423..26863)
                     /locus_tag="U4A80_001210"
     CDS             complement(26423..26863)
                     /locus_tag="U4A80_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1974842.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7381 a         5995 c         4693 g         8960 t
ORIGIN      
        1 tttttgtcac aacctttttt gccgagtaaa cggtgttcaa aagccagttt ctcggtatga
       61 agtcaaattt aagcaaaaat gtgagaagct attttcacaa aatttttctt catactcgaa
      121 actaagcggc tttttcacga cctctttttt attttgccat gaacatatca taatacgcgt
      181 ccattatttc tttagcaatc gtcaagttta tgtggtcttg ttcgtctaat aagttcggca
      241 agacaacact gatggcaatt tccggattgt ctgttggtcc gtatgccacg atattactat
      301 tgactgtcgc aatatctgga tgaccttccg caaccgtttc tgcagtccct gtttttgcgg
      361 aaaggtccat tttcgcactg gctaatggtt tggctgttgt gtaaggatcg gtaccatgga
      421 caacttggtg gaatccttca cggaggatcg ccatattttc tgcagaaata ttgaccgtat
      481 tttctactgt tgtgccaatt tctttcttca cttcacccaa gtttccattt tcgtcattgc
      541 catagatcgc tttgactagg tgcggctgta ctcgtttccc gccattagcg actgttgaag
      601 catattgagc taattggatt ggtgtatatg tgtcaaactg tccaaatgcc aaatcggtaa
      661 agttacctgg agcataccat tctttgccat tatccgcatt gaacttcttc atataatcaa
      721 cagaacgtga gatcccagga gattcattcg gcaaatcgat cccagtcgtt gtaccaagac
      781 caaactcttt gaaagagtct ctcaattgct gataagcttt agcttcttca tctaaactag
      841 gtagtcccat tccaggtgta tagtctagtc ctaacatctt taacgccact ttgatcatat
      901 acgtattaga agacaattcc gatgctttga ctgcatcaag agcaacttgt ccgcttcgat
      961 taaagacaga gcttttttct gaagcacctt gcagacgaat cggctcatca atcaaaactt
     1021 gatttccgct gatcgcgtta cttgcccagc ctgctgtcaa agtaccagcc ttgactacag
     1081 accctggatc aaaggcgcta gtaatcgtgc ctaaagcatt ctcggtgatt tctttggaac
     1141 cttgttcatg actgaaacca gtcattgcta gcacttctcc tgtctgagga ttcatagcca
     1201 ctgcataagc accaggcgaa tactgtgctt tgccgttgtt gatcaatgtt tgataattcc
     1261 ttttcaaaat ttcttctact tttgattgga attctgcatt catagataac attaaattag
     1321 aacctttttc cccagagaag atttcttttt gattcgaaac atttccttca ttatcaaggg
     1381 aaacttcata ttgggttttg gttccttgca ggacatcttc atattgtttt tccaaataac
     1441 tttgtcctac acggtcattc cgagaataac cttttgctag gtatttctct gcttcatctg
     1501 ctggaagtcc ttgcttttca gtcgtcacac tgccaagaat acttttcagt gaacttgctg
     1561 cattatattc tcgtgtccag tctgtgccag tcgaaactcc tggaagttca gaagcgcgtt
     1621 ctgccacaac agctaactct tgatcagtaa caccagagtt tttgatatag acagtgttta
     1681 attcatatgc actattcatc cgtttaaaga tggtagcaat ttttaattgc tcctcgttta
     1741 aattaatctc atcagctgtg actttattga ccatcgcact atattgttcg ctgttcccta
     1801 attgcttttc ttttgccgaa agtctttttg tggcttcttt caaatgtttg ggatcagcca
     1861 gccaaaaatc ttttttatct ctatctgtca gattttcatc taccggaaca tcgatcagcg
     1921 agttcagctt cgttgcgatt tcaagcaaat ctttagccgt aacttgtgtt cctctcgtat
     1981 atgtgatggc ctgattcgct ttattcgtaa ctaaagcttt tcctgtggag tcgtagatca
     2041 ttccgcgagg agtactttct tggaccgtaa tcgtagaagt agacttcact cgcgcctcca
     2101 tctcttcgcc gttcacgatc tgcaaataac ccaatctagc aatcaacgca acaaataacg
     2161 taaatataat gaaaaaaaga aaatttaagc gaaaaggaac atgggaccgg cgaaacgttt
     2221 tgttcttagg ttcttgtgca ccttttctta ttttttctaa ccaagaagta tttaatattt
     2281 ttttcataaa atctttcatc atttcctatc cttccgccta tttacattca tgcttcattg
     2341 tacccaaaaa cttcttaata aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa
     2401 tttcaagata aaatcaactt tttgtttcac agcaatcact taaagttaca tatccaacta
     2461 tttttttcat taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca
     2521 ctttgatgga tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa
     2581 aaaatagtaa ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt
     2641 tttcactttt tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat
     2701 cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg
     2761 tctagatgtt tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt
     2821 caacgcaatg atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc
     2881 acttctacct gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg
     2941 cgggagcaag acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt
     3001 ttgcagaata tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc
     3061 tgccgattcc aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt
     3121 ttcttttatc gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc
     3181 atttgcttct atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt
     3241 gattttgaag tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg
     3301 cgcactcaac ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat
     3361 attaccgata atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact
     3421 ttcaatacga ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag
     3481 tataccatat cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc
     3541 gcccaacaga atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg
     3601 aggaatacct gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat
     3661 ttacctatta gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga
     3721 tattcaaatc ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat
     3781 cttccaattc cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac
     3841 gtatcttata gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt
     3901 gcaaacgttc tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga
     3961 ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat
     4021 aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg
     4081 tattttcccc gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta
     4141 attgtatagt tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt
     4201 ttggtccgta aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca
     4261 tagcggcttt cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat
     4321 ctgtattgtt tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag
     4381 catatactgc caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac
     4441 gaacaaatgt atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag
     4501 cacctgattt ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac
     4561 gataagaatg gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa
     4621 gaacaagcat ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat
     4681 cccagtgtcc agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt
     4741 ggtaacctag actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg
     4801 ccccttttgg aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt
     4861 caagttcttt tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat
     4921 attctttcaa agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat
     4981 tgtttgagtt tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac
     5041 ggccagtgga tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag
     5101 cagtaatcac ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg
     5161 caaaaatttc taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt
     5221 tgacgatttt catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt
     5281 gtccattatc tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga
     5341 tatttggata gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta
     5401 agccaagtgc atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg
     5461 gacgagtcaa atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc
     5521 tttttgaaat gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg
     5581 caccatctgg aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat
     5641 cagatcatca agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac
     5701 gaaaagttcg tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg
     5761 gtccgaccgc ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg
     5821 gaaaagtttc agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc
     5881 tcatcaatca attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact
     5941 aaacttattt tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga
     6001 tgacatggat gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt
     6061 tcatcatatt ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg
     6121 gcataatgct ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg
     6181 tttgtcttgg gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc
     6241 aaaatagaga ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt
     6301 cccgtacttg caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt
     6361 ttcgctattc tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca
     6421 tctttcaaga tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt
     6481 gcaataccga tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc
     6541 gcaacaggtc tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag
     6601 ctacgggcat gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat
     6661 ccatccccgg tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc
     6721 tttgctgctt ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca
     6781 attgttacat agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca
     6841 cgtccaatct ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct
     6901 ttcagttgac taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct
     6961 aagatatggc tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt
     7021 attccattga ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt
     7081 ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca
     7141 acgatcccgt ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga
     7201 atcagtggac aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag
     7261 aaccaccaag aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga
     7321 tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc
     7381 atttgatact ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca
     7441 tttaaagacc ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct
     7501 gtcaaagaag attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca
     7561 agttcgccag gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc
     7621 tgcaattttc cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc
     7681 agttctttac ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg
     7741 atcaatatca tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta
     7801 atcgctaata catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac
     7861 atcaagaagg cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg
     7921 tgaaaaacaa attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt
     7981 ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat
     8041 ttccagctgc ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg
     8101 ccttaattga aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa
     8161 aaatattcag caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa
     8221 actatgctat acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag
     8281 aaacaactag aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat
     8341 atgctggata caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca
     8401 gctaatcaca agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat
     8461 cgccaacatg agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc
     8521 ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa
     8581 gaccgtgaaa cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa
     8641 acaatcgtta ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt
     8701 cccgcatttt atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa
     8761 tttaaaatcc aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat
     8821 gactttttgc tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct
     8881 acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt
     8941 gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg
     9001 tatcataatc gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg
     9061 ttttctgatt acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag
     9121 acattcaaaa attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa
     9181 caagaaagtg gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg
     9241 accttagtcg atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc
     9301 cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat
     9361 aaagaaacaa atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc
     9421 ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg
     9481 gcggaagaac agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa
     9541 aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga
     9601 atccatcgag ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt
     9661 ctacaaactg tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag
     9721 gaacatttga caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct
     9781 gtactattct ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc
     9841 tatatcttta ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc
     9901 ttgttcccaa aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat
     9961 gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa
    10021 ttaacgacaa gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg
    10081 aaagaacatc aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga
    10141 acaacagatg atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa
    10201 gcccggtatt ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat
    10261 gctcaactgc cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt
    10321 atgaagcgtt ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga
    10381 gttttcttgt gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt
    10441 tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt
    10501 cctttgttaa acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga
    10561 caactgctat cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa
    10621 gtaattcaat catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat
    10681 tttaatttga caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg
    10741 tcaaatacct tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc
    10801 cattcaatga attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa
    10861 atctctgata cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct
    10921 atcttctcat ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat
    10981 agtagaaaga aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa
    11041 cgacgaaaaa gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc
    11101 accatacact ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag
    11161 ccttcaacgc cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca
    11221 gctaactctt ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt
    11281 tttgcttcca tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac
    11341 ttatgctctc atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca
    11401 agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa
    11461 actggttgct tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc
    11521 ttccagtgca tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc
    11581 gttttgaggg atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa
    11641 tgcttcttgt atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc
    11701 ttccattaca tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc
    11761 gtcttctcct gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga
    11821 aacacccatg ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt
    11881 cacattgttc gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata
    11941 gcgcaattca gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat
    12001 aatatgtttt ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt
    12061 tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc
    12121 ataaatccgg ctattattag catctttagc agttttcttt gctacgatat ttgcccattt
    12181 acgtcccata ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg
    12241 ttccattata cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt
    12301 ctcattttta gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa
    12361 tattgcctcc caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct
    12421 ctttttttcc gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc
    12481 cagcatcggt aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt
    12541 gctttttgat attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc
    12601 tttttgtttt tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt
    12661 tcatgcgcga ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag
    12721 aagataacaa ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag
    12781 ttgaaagatc ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta
    12841 acgcatggcg aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat
    12901 agcctgcact tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt
    12961 cctttgcata acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg
    13021 gatacagact gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca
    13081 gttcttgaaa tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc
    13141 tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt
    13201 gcaagatttt tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg
    13261 gaagctccac tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat
    13321 cgatgatagc cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct
    13381 ctgctcctgt tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat
    13441 tgtactctac gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa
    13501 aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa
    13561 ctattctcgc aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat
    13621 atcgttcgga tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc
    13681 aaaaccgcta gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt
    13741 cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta
    13801 aataactttc caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac
    13861 cagacccttc ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc
    13921 ttcctggata actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt
    13981 ctggaaaaac ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt
    14041 cttctaacgc ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat
    14101 agcacagcga atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga
    14161 gcatattgcc ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa
    14221 tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg
    14281 caaccgctaa tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta
    14341 aatgacgttt cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt
    14401 aaatgaaagc aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta
    14461 gcggagcatc tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa
    14521 catgtacgat cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa
    14581 tacgttttac ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt
    14641 tgatttgacg ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat
    14701 ttcgaacgtc taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa
    14761 tttttaattg ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt
    14821 tgctacccag taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag
    14881 caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc
    14941 aatggctcgt gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac
    15001 ataaatgatt ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc
    15061 atgaagaatc tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag
    15121 accatgccag attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat
    15181 taccattttg aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat
    15241 actagctttt tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac
    15301 tccaagcaca tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt
    15361 ttctctagaa taagcagtat attcattact atagtccata ttcatacaac ccttcatttt
    15421 gaatataatc aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct
    15481 tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat
    15541 atggagtatc cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca
    15601 gctcatcgat tttataccat tttggcaaat attctaccat atccccaccg ataataaagt
    15661 aataatctgt atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt
    15721 tcccaccacg agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta
    15781 attccaacat gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg
    15841 gctgataaga aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag
    15901 ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct
    15961 ttttcttctc taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct
    16021 tagcttgcgt attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt
    16081 gatacttttc ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga
    16141 tttcgcaatc tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat
    16201 tttgaagtaa cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga
    16261 tcattgctga attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt
    16321 tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa
    16381 tcaatgcttt gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg
    16441 gcgctgttac agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag
    16501 aaaactcaaa acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta
    16561 ataatccacc tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa
    16621 tatttacatc atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa
    16681 atcttgcaag tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt
    16741 tgatttcttt taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt
    16801 gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct
    16861 cgattttatc caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg
    16921 ctgtattttt gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga
    16981 tatagacatc ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat
    17041 gagatttctt agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac
    17101 gttccttcat ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt
    17161 tgttcccaac taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg
    17221 aaccattgaa gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt
    17281 cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat
    17341 ggcgtaatct gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag
    17401 ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc
    17461 cgatacattg taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt
    17521 tgctaataga taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact
    17581 atctgttgcg ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg
    17641 tatatcagtc ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg
    17701 tttctttgct atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag
    17761 atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac
    17821 agggattcct gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg
    17881 attattccat gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt
    17941 ctttaattgg gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt
    18001 tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac
    18061 cccgtattat actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa
    18121 aaagcatttc tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca
    18181 aaaaacaact aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct
    18241 aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta
    18301 tatcgtggca actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac
    18361 tcgcgtagtt ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg
    18421 tctttccaca aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc
    18481 agcatccaga cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca
    18541 atgatgatgg cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata
    18601 gcctctcctt cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc
    18661 aaggtaatca ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga
    18721 tagccttcag gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct
    18781 ttttgaatac cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc
    18841 gcttcgtcat ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct
    18901 tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt
    18961 gctgtcttat ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat
    19021 tttctgtttc aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa
    19081 ttcagccttt tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct
    19141 tccagcaaaa ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa
    19201 actggcagtt actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc
    19261 attgctatgc cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc
    19321 ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt
    19381 tgctcgttcg aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat
    19441 aaaattagga tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt
    19501 gaggactgct tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc
    19561 gggaaatgct agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc
    19621 atcaataatc aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg
    19681 gcatactttt tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt
    19741 tgcccacatg ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt
    19801 gatccgaata tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc
    19861 tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat
    19921 gactgcagga tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt
    19981 gataaattca cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg
    20041 gacgattatt tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac
    20101 tgcactgtct actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc
    20161 tggagaagga gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg
    20221 tccttggaat actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac
    20281 ctgttttttt actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat
    20341 tcgagtattc atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt
    20401 tccggcattt tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc
    20461 tctttttgtt tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg
    20521 ttgtaaagaa caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat
    20581 ctgaacttct atatggcgag caggataaat gattttttcc aaatacatat catcatttcc
    20641 aaatgccgct gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga
    20701 ttgaactttc cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata
    20761 accaattctg tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc
    20821 tggaatgact gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat
    20881 tgcatcgatc gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga
    20941 ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt
    21001 cacgattgca gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg
    21061 gccaatgcag attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga
    21121 atatatcgct acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat
    21181 ttcaccgcgg ttagctatca atactttaga aaacatattt cacctatcca atcataaagg
    21241 ttaattccgc ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg
    21301 ctgaagcttt cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt
    21361 tcttacggaa ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt
    21421 caagagaaag taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg
    21481 gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt
    21541 tcttagcaac gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg
    21601 gataacgatg tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct
    21661 gctttgctta tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt
    21721 tttctacaag gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt
    21781 tcatcgcttc aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga
    21841 aagctggttt gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct
    21901 cgattccatc aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt
    21961 gttcaggtat gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat
    22021 tcatgcctcg acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat
    22081 cgaattccgt tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat
    22141 ccaaaattat tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa
    22201 tgaattactc attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa
    22261 gtcaatctca ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa
    22321 tgccagcaaa gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat
    22381 cgatttcgtg ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc
    22441 ttttgcttct gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac
    22501 ttgttccggc tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga
    22561 accatctgga gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat
    22621 ttctccatag attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac
    22681 gcctgctcct tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg
    22741 attaggatca gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc
    22801 acaaatcgtt gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt
    22861 acggaatgct tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca
    22921 aatacctttt gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat
    22981 tggtacgaac attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt
    23041 ttcgattgtt ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt
    23101 cattttttct ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata
    23161 gatcgaaaac atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa
    23221 atctttgact tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg
    23281 tgcgatgcca ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat
    23341 aggtgaagtg actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc
    23401 ttatccgtgc atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt
    23461 ttgtgctaag aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg
    23521 aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt
    23581 gatgaatcct ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac
    23641 agattttgtt aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt
    23701 tccaatcaac ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat
    23761 ttttttcaat acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt
    23821 tttgaaatct tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt
    23881 tactaacaca tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt
    23941 ctcataatca gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc
    24001 ttctatcttt tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt
    24061 tgcaaaagct aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt
    24121 ttttcctttt acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc
    24181 tgcttattct caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt
    24241 aatacttttc cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg
    24301 atactctcat agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt
    24361 tctttcttca ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat
    24421 tgaatctgac ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga
    24481 aaaggcccgc tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa
    24541 acagcttcgt ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa
    24601 tttgcaggag atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca
    24661 atcggcgcat tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata
    24721 taagcgcctc tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct
    24781 actaatgccg aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc
    24841 tccttcaata agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc
    24901 gtctgatcca aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc
    24961 tcacttgctt catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata
    25021 ccttgatatt gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat
    25081 atatccagaa tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag
    25141 gaaatgtccc agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt
    25201 cactaaatct tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc
    25261 ttcaatttca ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat
    25321 gtcagtagtc aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg
    25381 aattttttcg aataccataa taatatattc ctccgatatt gtttgctttt tatttttata
    25441 atttaacagt ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg
    25501 taaatattac tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca
    25561 ataagatagg gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact
    25621 tttcttgcgg catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat
    25681 gcaaaagcag ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt
    25741 ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg
    25801 aatcttctga atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc
    25861 tggtaccgtc agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga
    25921 gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta
    25981 gcttagatag tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt
    26041 tttcagccat gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga
    26101 tagcatgagt tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat
    26161 cgggcgacat tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt
    26221 ttatcaattt ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc
    26281 ttcgttcacg aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg
    26341 aaagatcatt attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc
    26401 caaattgttc cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca
    26461 gtgcatccag caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca
    26521 tttcacgatg aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca
    26581 actttacaac gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta
    26641 acttgttaat agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg
    26701 aagttgtttt tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca
    26761 gatctttgaa ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga
    26821 cactaacaag ataatcattt actgttttta aatctggttc catgttccac cgtccactaa
    26881 ttagtttgat tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca
    26941 accttaaaag atcatttttt tactatatga gcttttttta agattttact catttaaaac
    27001 aactgataaa tcagcattta taatttctt
//
LOCUS       DASJRQ010000032        26921 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJRQ010000032 DASJRQ010000000
VERSION     DASJRQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.032"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..1092
                     /locus_tag="U4A80_001211"
     CDS             88..1092
                     /locus_tag="U4A80_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974843.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1093..2037
                     /locus_tag="U4A80_001212"
     CDS             1093..2037
                     /locus_tag="U4A80_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974844.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2037..2999
                     /locus_tag="U4A80_001213"
     CDS             2037..2999
                     /locus_tag="U4A80_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974845.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3026..3940
                     /locus_tag="U4A80_001214"
     CDS             3026..3940
                     /locus_tag="U4A80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974846.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3966..5747
                     /locus_tag="U4A80_001215"
     CDS             3966..5747
                     /locus_tag="U4A80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974847.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6095..6781
                     /locus_tag="U4A80_001216"
     CDS             6095..6781
                     /locus_tag="U4A80_001216"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1974848.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6801..10382
                     /gene="smc"
                     /locus_tag="U4A80_001217"
     CDS             6801..10382
                     /gene="smc"
                     /locus_tag="U4A80_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1974849.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10391..11200
                     /locus_tag="U4A80_001218"
     CDS             10391..11200
                     /locus_tag="U4A80_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1974850.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11211..12209
                     /gene="ftsY"
                     /locus_tag="U4A80_001219"
     CDS             11211..12209
                     /gene="ftsY"
                     /locus_tag="U4A80_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1974851.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12396..14663
                     /gene="gshAB"
                     /locus_tag="U4A80_001220"
     CDS             12396..14663
                     /gene="gshAB"
                     /locus_tag="U4A80_001220"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1974852.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14752..15204)
                     /locus_tag="U4A80_001221"
     CDS             complement(14752..15204)
                     /locus_tag="U4A80_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1974853.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15354..16304
                     /locus_tag="U4A80_001222"
     CDS             15354..16304
                     /locus_tag="U4A80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1974854.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16297..17256
                     /locus_tag="U4A80_001223"
     CDS             16297..17256
                     /locus_tag="U4A80_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1974855.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17253..18008
                     /locus_tag="U4A80_001224"
     CDS             17253..18008
                     /locus_tag="U4A80_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1974856.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18031..18984
                     /locus_tag="U4A80_001225"
     CDS             18031..18984
                     /locus_tag="U4A80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1974857.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19628..19924)
                     /locus_tag="U4A80_001226"
     CDS             complement(19628..19924)
                     /locus_tag="U4A80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1974858.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20279..20635
                     /locus_tag="U4A80_001227"
     CDS             20279..20635
                     /locus_tag="U4A80_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1974859.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20645..21544
                     /locus_tag="U4A80_001228"
     CDS             20645..21544
                     /locus_tag="U4A80_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1974860.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22061..22792
                     /locus_tag="U4A80_001229"
     CDS             22061..22792
                     /locus_tag="U4A80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1974861.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22937..24964
                     /locus_tag="U4A80_001230"
     CDS             22937..24964
                     /locus_tag="U4A80_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1974862.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25072..26169
                     /locus_tag="U4A80_001231"
     CDS             25072..26169
                     /locus_tag="U4A80_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1974863.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26476..>26921
                     /locus_tag="U4A80_001232"
     CDS             26476..>26921
                     /locus_tag="U4A80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974864.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT         9023 a         4663 c         5685 g         7550 t
ORIGIN      
        1 aacctttgtt ataaaacgct ttattctgtg taatgtcctt ttttattaag aaaaacttcc
       61 atttaggaag cagagagggg tattcgattg tccgataacc aattattaga agttcagaac
      121 ctacatacag gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt
      181 actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc
      241 ttggctacta cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt
      301 ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga
      361 aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc
      421 gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa
      481 gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc
      541 caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc
      601 gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc
      661 caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc
      721 ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat
      781 ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct
      841 tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat
      901 gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca
      961 ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc
     1021 gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa
     1081 ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt
     1141 cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt
     1201 attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact
     1261 acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga
     1321 aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg
     1381 atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct
     1441 gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga
     1501 ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca
     1561 ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc
     1621 gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc
     1681 atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt
     1741 gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc
     1801 ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca
     1861 gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta
     1921 gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat
     1981 ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt
     2041 ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc
     2101 tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa
     2161 atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt
     2221 atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct
     2281 tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat
     2341 cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc
     2401 ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg
     2461 ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt
     2521 tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag
     2581 accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca
     2641 caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag
     2701 cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag
     2761 cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag
     2821 ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc
     2881 aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg
     2941 ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa
     3001 accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag
     3061 tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat
     3121 tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac
     3181 cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt
     3241 ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg
     3301 acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac
     3361 ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa
     3421 catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt
     3481 cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt
     3541 ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga
     3601 ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat
     3661 catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat
     3721 cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt
     3781 aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg
     3841 gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc
     3901 attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa
     3961 taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag
     4021 catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca
     4081 aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg
     4141 aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt
     4201 accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg
     4261 atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga
     4321 aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg
     4381 cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt
     4441 atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca
     4501 caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa
     4561 acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt
     4621 tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga
     4681 cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa
     4741 acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga
     4801 tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa
     4861 cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa
     4921 cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg
     4981 aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat
     5041 tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat
     5101 tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc
     5161 tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt
     5221 taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg
     5281 aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg
     5341 tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg
     5401 atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa
     5461 gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata
     5521 caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag
     5581 catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc
     5641 gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac
     5701 caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga
     5761 ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata
     5821 ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc
     5881 tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt
     5941 catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc
     6001 tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag
     6061 tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact
     6121 aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca
     6181 ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt
     6241 tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca
     6301 tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc
     6361 gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa
     6421 ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc
     6481 gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc
     6541 taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt
     6601 cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc
     6661 tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca
     6721 agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta
     6781 aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat
     6841 ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg
     6901 gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta
     6961 aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc
     7021 agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg
     7081 agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca
     7141 tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag
     7201 gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac
     7261 cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga
     7321 aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca
     7381 tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg
     7441 agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa
     7501 tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag
     7561 aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta
     7621 aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt
     7681 cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata
     7741 cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac
     7801 attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc
     7861 aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa
     7921 cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag
     7981 ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca
     8041 agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa
     8101 aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga
     8161 agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc
     8221 ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc
     8281 aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata
     8341 aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt
     8401 tcacgttagc tatcgaagca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg
     8461 aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat
     8521 tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag
     8581 cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc
     8641 aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca
     8701 atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga
     8761 tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt
     8821 ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac
     8881 tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag
     8941 aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa
     9001 atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa
     9061 cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat
     9121 tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat
     9181 cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag
     9241 cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc
     9301 tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc
     9361 agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa
     9421 aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca
     9481 aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc
     9541 gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc
     9601 gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat
     9661 ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg
     9721 tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac
     9781 aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag
     9841 cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat
     9901 tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg
     9961 gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg
    10021 agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag
    10081 aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt
    10141 tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc
    10201 gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa
    10261 cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa
    10321 tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt
    10381 aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca
    10441 aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa
    10501 gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg
    10561 actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga
    10621 tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa
    10681 actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct
    10741 gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca
    10801 accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta
    10861 tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta
    10921 tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc
    10981 ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat
    11041 cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga
    11101 gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa
    11161 tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt
    11221 ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg
    11281 agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt
    11341 tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta
    11401 tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac cgaagcatta cgtcaagaag
    11461 tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg
    11521 tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg
    11581 gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta
    11641 aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct
    11701 tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg
    11761 ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa
    11821 agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga
    11881 acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac
    11941 cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa
    12001 aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag
    12061 caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg
    12121 gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat
    12181 tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa
    12241 agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat
    12301 ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga
    12361 ttataattag attcatatga attggaggtc acttaatgat gaattttaag caattattat
    12421 tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac
    12481 agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc
    12541 gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc
    12601 ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg
    12661 ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg
    12721 aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac
    12781 gttatttagc aaaaaaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt
    12841 ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt
    12901 tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct
    12961 ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg
    13021 aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc
    13081 atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa
    13141 tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag
    13201 gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata
    13261 tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat
    13321 tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag
    13381 aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc
    13441 atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt
    13501 tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt
    13561 tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat
    13621 tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg
    13681 aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca
    13741 aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg
    13801 tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca
    13861 aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat
    13921 tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga
    13981 tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc
    14041 tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag
    14101 aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca
    14161 tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg
    14221 ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga
    14281 tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag
    14341 ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg
    14401 attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg
    14461 tagaagcact aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg
    14521 atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc
    14581 atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat
    14641 tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc
    14701 aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa
    14761 acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc
    14821 ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa
    14881 gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct
    14941 attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt
    15001 caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt
    15061 tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa
    15121 atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg
    15181 gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac
    15241 gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa
    15301 cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct
    15361 acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat
    15421 cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag
    15481 tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg
    15541 ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga
    15601 tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga
    15661 ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc
    15721 ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag
    15781 ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata
    15841 tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt
    15901 atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg
    15961 gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag
    16021 gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt
    16081 ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa
    16141 caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc
    16201 gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg
    16261 ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt
    16321 tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca
    16381 tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt
    16441 gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt
    16501 ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc
    16561 ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac
    16621 gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga
    16681 gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc
    16741 agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta
    16801 tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc
    16861 ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat
    16921 gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt
    16981 gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt
    17041 attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc
    17101 ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt
    17161 ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc
    17221 acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac
    17281 tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaagttga
    17341 ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt
    17401 tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat
    17461 ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa
    17521 tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga
    17581 caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa gaaatagcgg
    17641 atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc
    17701 tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt
    17761 ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg
    17821 ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga
    17881 aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg
    17941 atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt
    18001 atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt
    18061 ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca
    18121 aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa
    18181 atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg
    18241 atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag
    18301 tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa
    18361 aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa
    18421 aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg
    18481 gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct
    18541 aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct
    18601 actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct
    18661 ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc
    18721 gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat
    18781 atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt
    18841 tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt
    18901 gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa
    18961 gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta
    19021 acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa
    19081 accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt
    19141 ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa
    19201 aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt
    19261 acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc
    19321 tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga
    19381 gaagctattt tctgtccagc gttcttatta ctcaaaacaa atcgcagttg tcacaatctc
    19441 ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg
    19501 agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct
    19561 cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa
    19621 cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat
    19681 aaaaatcggc gggaagctta ctccaccgtt ttagtaattg atagaaataa ctctcttctg
    19741 ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt
    19801 ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa
    19861 tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct
    19921 tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt
    19981 taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt
    20041 ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt
    20101 tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat
    20161 aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca
    20221 gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat
    20281 gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga
    20341 tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg
    20401 ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag
    20461 tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat
    20521 actttacatg gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt
    20581 aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga
    20641 taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga
    20701 atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct
    20761 agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa
    20821 taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga
    20881 acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa
    20941 tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa
    21001 agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg
    21061 tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga
    21121 ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc
    21181 atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg
    21241 atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg
    21301 ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc
    21361 ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct
    21421 agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca
    21481 ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta
    21541 ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag
    21601 agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg
    21661 tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt
    21721 ttgaacgata atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat
    21781 tgattgaagt ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta
    21841 ttttaaaggc tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac
    21901 tagaaatcaa atcgggctga taatcattta taactaatcg atacatagga ccaaatatag
    21961 gtctatgttc ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa
    22021 tcgactgata tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt
    22081 attaacagca gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac
    22141 tgaagtatac ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga
    22201 gggtgtaact cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc
    22261 agatcctcca agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa
    22321 atttgggact caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat
    22381 gaacgatggg tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc
    22441 tgggtggaaa ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact
    22501 agttggagca cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata
    22561 tacgccgcaa acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc
    22621 aattaatgct aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga
    22681 aaatgaaaat caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca
    22741 acaatattcc actaaattag tttggacatt agaagataca cctaacaact agtttactga
    22801 aaagaactgt taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat
    22861 tgtcaaaggc taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc
    22921 aggaggaaaa gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt
    22981 gactaatatc tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa
    23041 tagaagagac gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca
    23101 gacacaagaa agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt
    23161 agaagctgag ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct
    23221 gcctgacgat cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag
    23281 cacgtccata ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag
    23341 aggagctatt tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta
    23401 tttatactta ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg
    23461 cgatcctaga atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg
    23521 ctcatattct actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga
    23581 tacgtataaa aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag
    23641 agggcatgga catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga
    23701 gcatactggc gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat
    23761 aattaaatgg gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcgctc
    23821 ttctcggatt atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt
    23881 tacttcttca acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc
    23941 gaatgtcaca tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga
    24001 agctggagga aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct
    24061 tgaatataag aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca
    24121 tatcttgatc aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt
    24181 cacgctaaat ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac
    24241 aactttgcag gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa
    24301 cgggaatttg attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat
    24361 agatatccat gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac
    24421 aatcactggg aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt
    24481 ttttgactcg gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta
    24541 tttttattac agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg
    24601 aaaacataaa atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa
    24661 aattatagat gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct
    24721 tacaaatgga gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac
    24781 ggttataggg acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt
    24841 gtcctctctt ttagattcta cagctcatcg tggaatcaga ataaatattc ctgtagaaaa
    24901 ccaaagaatc ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa
    24961 ttaacaggaa gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat
    25021 aggctcttgt gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa
    25081 ggagcgattt tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg
    25141 gcacttttta acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata
    25201 acttcagaga atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat
    25261 caggaagtag aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat
    25321 atttctgtga atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag
    25381 attgaaaaag acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata
    25441 gtgacgctaa aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag
    25501 cgatttgatg gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac
    25561 caaacagaaa ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct
    25621 ttgttgatgc aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa
    25681 ccagatcaaa tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc
    25741 tatatcaatc aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta
    25801 tatcaagagg aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact
    25861 gctttaaatg gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga
    25921 aatgaaaatg aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc
    25981 aatcaatggg tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac
    26041 acgacggatg ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt
    26101 ttcttattgt ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa
    26161 gaaaagtaag gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct
    26221 ttttataaca atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat
    26281 aattctaata acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa
    26341 aaaaaagaca ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat
    26401 ccaggaaatc agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga
    26461 aagagctgat gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat
    26521 tcattactta aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa
    26581 gaagagttgt cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat
    26641 ttacaaaagt tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt
    26701 ctggaaatta atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct
    26761 ttatctatga aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt
    26821 gctaaacagc atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga
    26881 aatctaaaaa aatatcaaat tttttttgaa aagagaaagt t
//
LOCUS       DASJRQ010000033        26775 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJRQ010000033 DASJRQ010000000
VERSION     DASJRQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.033"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(115..693)
                     /locus_tag="U4A80_001233"
     CDS             complement(115..693)
                     /locus_tag="U4A80_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1974865.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(713..1381)
                     /locus_tag="U4A80_001234"
     CDS             complement(713..1381)
                     /locus_tag="U4A80_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974866.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1396..2466)
                     /locus_tag="U4A80_001235"
     CDS             complement(1396..2466)
                     /locus_tag="U4A80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974867.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2637..4001)
                     /locus_tag="U4A80_001236"
     CDS             complement(2637..4001)
                     /locus_tag="U4A80_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1974868.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3998..5557)
                     /locus_tag="U4A80_001237"
     CDS             complement(3998..5557)
                     /locus_tag="U4A80_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974869.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5613..8093)
                     /locus_tag="U4A80_001238"
     CDS             complement(5613..8093)
                     /locus_tag="U4A80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974870.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8077..8412)
                     /locus_tag="U4A80_001239"
     CDS             complement(8077..8412)
                     /locus_tag="U4A80_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974871.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8426..9460)
                     /locus_tag="U4A80_001240"
     CDS             complement(8426..9460)
                     /locus_tag="U4A80_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1974872.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9550..10230)
                     /locus_tag="U4A80_001241"
     CDS             complement(9550..10230)
                     /locus_tag="U4A80_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1974873.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10400..11359)
                     /locus_tag="U4A80_001242"
     CDS             complement(10400..11359)
                     /locus_tag="U4A80_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1974874.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(11580..12683)
                     /locus_tag="U4A80_001243"
     CDS             complement(11580..12683)
                     /locus_tag="U4A80_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1974875.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12905..13375)
                     /locus_tag="U4A80_001244"
     CDS             complement(12905..13375)
                     /locus_tag="U4A80_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1974876.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            13632..14951
                     /locus_tag="U4A80_001245"
     CDS             13632..14951
                     /locus_tag="U4A80_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN1974877.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            15392..17032
                     /locus_tag="U4A80_001246"
     CDS             15392..17032
                     /locus_tag="U4A80_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1974878.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            17186..18508
                     /locus_tag="U4A80_001247"
     CDS             17186..18508
                     /locus_tag="U4A80_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1974879.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(18570..19001)
                     /locus_tag="U4A80_001248"
     CDS             complement(18570..19001)
                     /locus_tag="U4A80_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974880.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(19065..19901)
                     /locus_tag="U4A80_001249"
     CDS             complement(19065..19901)
                     /locus_tag="U4A80_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN1974881.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(20091..20393)
                     /locus_tag="U4A80_001250"
     CDS             complement(20091..20393)
                     /locus_tag="U4A80_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1974882.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(20419..20850)
                     /gene="ruvX"
                     /locus_tag="U4A80_001251"
     CDS             complement(20419..20850)
                     /gene="ruvX"
                     /locus_tag="U4A80_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1974883.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(20847..21113)
                     /locus_tag="U4A80_001252"
     CDS             complement(20847..21113)
                     /locus_tag="U4A80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1974884.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(21367..22092)
                     /locus_tag="U4A80_001253"
     CDS             complement(21367..22092)
                     /locus_tag="U4A80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1974885.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(22458..22528)
                     /locus_tag="U4A80_001254"
     tRNA            complement(22458..22528)
                     /locus_tag="U4A80_001254"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22494..22496),aa:Gly,seq:ccc)
     gene            22645..23397
                     /locus_tag="U4A80_001255"
     CDS             22645..23397
                     /locus_tag="U4A80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1974886.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            23413..23892
                     /locus_tag="U4A80_001256"
     CDS             23413..23892
                     /locus_tag="U4A80_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1974887.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24143..25723)
                     /locus_tag="U4A80_001257"
     CDS             complement(24143..25723)
                     /locus_tag="U4A80_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1974888.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(25716..26459)
                     /locus_tag="U4A80_001258"
     CDS             complement(25716..26459)
                     /locus_tag="U4A80_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974889.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(26635..>26775)
                     /locus_tag="U4A80_001259"
     CDS             complement(26635..>26775)
                     /locus_tag="U4A80_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEN1974890.1"
                     /translation="NTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENE
                     SF"
BASE COUNT         7889 a         5423 c         4703 g         8760 t
ORIGIN      
        1 gagagaataa caaatttgta tttatgaaaa actacctata taaagaagca agatataacg
       61 atcttcgtca tatcttgctt cttcacctaa ctattttctc cctatataac agctttacgc
      121 ttctcctaat acaccattca aatataaatc gatcaattgc gccacattta gcggttcttc
      181 cacaggtctt ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat gccttgctgc
      241 gatttctttt gaaatcccat ttcttagcac agattggttt gctgaaaaga tttcttcaaa
      301 aggttcacga taatttttct tccatagaaa gaaaagatga tctcttgttt cctttgaaag
      361 attgctttca agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa
      421 ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct
      481 caaagctttt ccaacatctt ttaagtggtt ttctaaaact gccgtatata gtttttcttt
      541 atcaccgaaa taatgataga gattgggctg ggtgatccca actttttgtg cgatttctct
      601 tgttgaagtg ccgagatatc ccttggacat aaacaagtca gtcgcttctt tcaaaatttt
      661 ttgctgtgta tctttgttgt atcgatggac catttttcca cctgcttttt ggttattctt
      721 tttctttcaa tacaccatct tccatcataa atattctatc gcatttgtca atcagccgtg
      781 tatcgtgagt aaccatgatc gttgctttat ttttgtcttt tgattctttt gccaagatat
      841 cgactacttc tcgtgccttt tctgaatcca aactagcagt tggttcatct gctagaataa
      901 tggacggatc attgtacaat gcacgagcga ttgcgaccct ttgccgttct ccaccagata
      961 gttcttctgg atacttgttg atcaacttat ccacgccaag ctgctcaaat aattcgtttg
     1021 cttctttttg acgatgttca tgtttaatct tatctactaa taataactgc ttttttactg
     1081 tcagaaaagg aaccagattg gaagcttgca aaataaagcc aatttcttta aaacgtagtt
     1141 taacgcgttc gctttctttt tgattactga attcttgatc attgataaat acagagccac
     1201 tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt acttttacct gaaccagaag
     1261 gaccgatgat tgcaacaaat tcccctttat cgatactcat ggttgtttct ttcaatgctt
     1321 taactacttg gtcgccatct tggaaacttt tttctacttc tttaaattct attgctttca
     1381 tgttcttcct ccttcttatc caattgcctt caacggatcg atttttacga ttgtccgaac
     1441 agaaaatagt gcgcctaaca cagctactac taacatcaac aagctaatga cgccaaaaaa
     1501 cagccaattg ctttggaatg gaaccgcatc aggtagaaca agggatgttc ccactgttcc
     1561 taatagacca agaagaatac caacaaatga taaaatgaat gtttgtgcca ctacagaacg
     1621 cgcaataaat cctcctgtga tcccttgtgc tttcattaca ccaaagatat taatcttttg
     1681 catcgtcagt acataaatga aaatcccgat cacaatagcc gcaataacga tcaaaaatcc
     1741 gatcataaat ccaaaagtca atacttgcgc attatatcca ggtaattcat tgatgaattt
     1801 agaaattttg atctgttcta aatcttcagg taaattactg attttacctc tgacaactat
     1861 tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg aagcggattt cttggtaagc
     1921 attgatggtt gtatagagaa ctggtgagac attgaatttg gcatgatccg taaagccagt
     1981 tagtttaaac gtcttgtcac taccagataa cttaactgta tctcctatgg ctagaccata
     2041 ttcttctttc aaactaatat cgcctactgc ctcatcatcg ttatcaaaag cttttccttc
     2101 aacaagattg ggcatgatga attgattttt atcgataccg aagaagctaa cattgatttt
     2161 tccttcttct gtactgtctt tactagtcac gactccaggt gtttgggcaa gatacgccac
     2221 tttcccacct tcgacttctt ctgctatctt cttagtaatc atggacatcc ctagatttga
     2281 atttgcatct ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt
     2341 atcttgagct aagccataag ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa
     2401 catcacccct gcaactaacg cataacgtaa ctttgaatgc ataatttcat ttaatgctaa
     2461 aaacatttaa ctcctcctcc tgaaataatc attttataca acaattttat cacttgataa
     2521 atttttggcg aatattatgc tcaaaaaatt cttccgctat ttcattatat gaatttttta
     2581 ttctcgattg attaatattt attatatccc cacacaataa aagaccgtga tatttctcac
     2641 agtcttttat ctactctatt gctatttgct ctcttagcta gtactttaaa agctaactca
     2701 ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc caaaatcctc aactgctagc
     2761 tgtggaatcg tagttttgtg tttcacgata tccgcaagca ggttcaaatc tgataataag
     2821 aattcttggc ttatcagctg tccgccaatt atttctaatg tatccggatg gtagatcagc
     2881 tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa atagatcaaa ttctcctgaa
     2941 cacgaaccgg tattttgttc caagaatggc gcttcatctt ctgtcagccc tacagttcct
     3001 aaaaagtagt ctataatgac tgctgctgtt atcttttgtc tgaggtcgta agcggttggc
     3061 tgaccactca aagttagcgc tactgctctt gccatatgta cagctcgagt aactaaaggt
     3121 aaaaagagat gccgtttatt tttgtcaaaa ctgactggga cgatatctcc aatcgcatag
     3181 atgtccggat cacttgtctg cagatattca ttgactatca cagtctcatc ctcatacaaa
     3241 gccacttttt cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa aatgaccata
     3301 tctgccttta ttgtttgaga cagaagttgt acggatacta ctttctcttc tgattttttc
     3361 caatcgatca agaattcatt tggataaaaa tttatttgct tagtttgcag ctgattttga
     3421 aaactgttga tcatatcctt atcgaaatat cttgataaaa cattcggcat ataatcaact
     3481 agatgaataa tgtatccttt atttctcaaa gcatctgcta attccaatcc aatgtaacca
     3541 gcaccgaaga tcactacttc ttttgcttct tttaaagcgg caagtacttg cttagattgc
     3601 actagatttt tataggtgta gacattttca gcttcaattt gtttatacaa ggtgacagga
     3661 gaggaacctg tcgctacgat taatttatca taggttatgg tctcgttttt tccttctttg
     3721 gatgcgagga tgagctgatg tttgtctgga tcaagttcag ttactctcca ttcggtgatc
     3781 agttttgccc cactttcttg aagagtccgc atgttatggg caacttcagc taaactacgt
     3841 atctttcctt ttaaataaag gttgattcca tttgaaacat agcctagtcc atctttatga
     3901 tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt tagggcagct
     3961 gtgatgcctt catgggaagc accaatgatc acgattttca tgttttattc atccttaaat
     4021 tcaatttttc taaataacat tcttttaaaa attggttcaa ataggggaaa tcataattat
     4081 attcgttgct agtgaataca aaaactttag cggacttctg tgacgggtac tctttcaatt
     4141 ggctagtcaa aatgatatca tactgctttt ctttctcgta ttcgactaaa tcatatttga
     4201 tcgtattcag caattgttcc tggatgattt ctttcacgat atccgccatt agcctttcat
     4261 aaggaagatc ataagcaatt tgcagacgta attctaactg cttgatcagc gtccgtaaaa
     4321 tagatgcata gcgagcaaat aattcaaagc gggcattttc ctcgtctact tcagtaaaat
     4381 caacaatagc aaatgtttgt ctgaccaatc tttcacataa aaccaggaat tgtttatctt
     4441 ctacttcagt taaccgctgt tttaatcctt cttggccaaa aatattcatc catcctctaa
     4501 aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt
     4561 ctggaaaaac ataaaaagag aaatattctt ctatcgattg tttaaacgtt cgattcaatt
     4621 gagctactac gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat
     4681 gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt tcattttgat
     4741 tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa
     4801 attgtgtcgt ttgaaacaat ccaacaggaa gattgctata atctggtttg gattttttcg
     4861 tacagcgcat tcgcttcttc ataatggaaa gccatatttc aaatttcaat cgtccttctt
     4921 ctgtaatagc aaaactaaac tctttttgca gcagatctaa aaaagtgttt cctgataaat
     4981 cactgtattc ttttttgttt tcaagatagg gacgtccaaa ccaaaataat tggaaataaa
     5041 aataactaat ttgactttct ggaccggtca gccgcccgct ttttattttc agatgaaact
     5101 catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa ctgatattaa
     5161 gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa agaagtatct
     5221 gatattttgt agaatagcgc aagtaacaat gataataaac atcgactgct gctatatttc
     5281 catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt ttgatatttt
     5341 ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca actgataagc
     5401 gggtaagtac gttattaacg gaaacctctc ctttttcctc taataaaagt tggaaaagtt
     5461 tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc actccttcaa
     5521 gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag gatctgtgac
     5581 agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc tgtcagttcc
     5641 catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct atttacctct
     5701 aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc tgttccatca
     5761 ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gctttgatcc agaatgattg
     5821 aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa agaagtcaat
     5881 gtacctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac atcaaaagaa
     5941 aaatcgcctt ttgattcata ataacttgtc ttcaacggtt tggggagatc gttaccaaat
     6001 gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag aattgtttta
     6061 gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt aatattgctg
     6121 gaaagtacag ctgcctttgt cagtatgctg acggattgta aagaagtatc tacagtattt
     6181 aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag atctgctggg
     6241 acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa ttttaaggaa
     6301 ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc ggttatcgca
     6361 tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca
     6421 gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact atctgcactc
     6481 acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc ttgttcagac
     6541 tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac caagcgataa
     6601 tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt atttacaccc
     6661 ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg
     6721 gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt tgctgaccaa
     6781 ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact atcggctgtt
     6841 aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt taaagcaaca
     6901 ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc tgtctgattc
     6961 aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac gttttttcct
     7021 gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa acgtccttca
     7081 gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg gttatttcca
     7141 tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc acttggtgag
     7201 atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc tcctggattc
     7261 aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa aatcgaattg
     7321 aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa aacggccttt
     7381 ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact cgtatcatat
     7441 ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg aagcgggata
     7501 tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa tatctgtcaa tagagtattt
     7561 gtatcccacg accccatccc aaaacgcaag gtatggcttc ctgctgataa taaacttccg
     7621 ctagtaacct tgatgggggc accggcaata tacaagtagg cgttgttcca gtcaattgct
     7681 tttaatacat cactgcttga tacatgttcc ggcaaattga tgatcgccac aaatgcaaat
     7741 ccttgtgcag tgatattcac caaatcaagt acatttgtta gtttcacatg gctttcccaa
     7801 tgaaaacgga ccgtgccacc tttccaagga tcatttgaac catcgttcaa ataagtagca
     7861 gtcaatttag gcaattggtt agccccagtt agttggacaa gcttcccgcc attgactgca
     7921 ctacgtccac catttggagg tacgatattt tcatacgtgt atggatcata agaaatggca
     7981 ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa
     8041 agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc
     8101 ctccttttcc tttcttctcc ataacaacaa ccacgcaaga agccataatt ctataccaat
     8161 gatcaaataa agaaaacgga tcttttgttc attcgtctcc gggaatacgc catgttgatt
     8221 agattcttta taaatcggta gctgtgtctg ctgttttgat gcgccattga ctgaaccact
     8281 tggatgcgga attggactta gatcataaaa tgtaatcgta cctgaacttt tggtatctgc
     8341 ttggactggc tcttgccagc tgaagaacag caaacttgca ataatccctc ccaataagat
     8401 cgatgttttc aacgaaatca cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga
     8461 cccacaaaaa cagcaaaatc aaccataaca ctagaactgc agcaaccaat cctgcataaa
     8521 gccatgggaa agattgttct tgtactgcaa aaacggaatt ttcatttact ttttgtgctt
     8581 tttctttttt gatcgtaaag ttttgtgtaa atcgccattc attactgtcg cctgattttg
     8641 ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc accagctaaa tcaagattca
     8701 agtgaaaatt cgaatttggt gcaactaaaa atgcattaga cgtattttca atataaagct
     8761 gattttcata aaagatcttg ctcgtaaata tcaaattgct gataatccta ggctgaggat
     8821 tttggaaacg aagctgaaca gttggctttg cgttgacctc gtccaaatct cctgataaaa
     8881 gtttcagatc cggttctatc gtctcttgac tttcttgtaa aacgactgcg agattataag
     8941 caatacgatt tttgatttgt gctccttctg tagcattttc tgtttcagct tcttctttag
     9001 gtgaaacatg aatacccgct aatacacgtc ccttaaagct ttttgctggg attttcactt
     9061 ctataggtac agttgtttct gcatgtgctg atacattgat ttcctttttc gcaatgagca
     9121 aaacgtcacg gacatcaaac ggcaatgtct catctaacgt tgggtttttt tgactataat
     9181 cgatcgttcc accatcactc gttaccgccg gattgacaga gacctcaaat gttcgttctt
     9241 catctgaaga attgctgata attacttcta gtgtctgatt ttgttccggt gccactaata
     9301 aatcaaaata accgattgct ttatctattt ggttctctgg aaataccggt ttgattccaa
     9361 agtctcctgc tgaatccttt gcttggctga ttgacggaaa aatgaaaaat agacttaaaa
     9421 tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat tgtccaccac atcctttcaa
     9481 caacaactgc ggtaaaaaat ccttcaccta tagtattttc accgcagttt gttgaattta
     9541 tttcatactc tatgctactg ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt
     9601 tccagctgct gctttcggca ctttcagtgt gatatttgct ggatcataaa cagaaagcca
     9661 tgttcccaga ccttgatctt ttgctgctgt aaatattggt gtcgctgcac ttgctttact
     9721 caacccagtt acagtttgac tagttggtcc agtggattcg ttatctgacg tacttttaac
     9781 agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc
     9841 tgaaaccgaa actgtcacgt tccacccttg tgcttggcca tctgcaccta cacctcgacg
     9901 atctgatact tggagataag gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt
     9961 tccgctttca atctcgtgtg taccaaatgg caattcggga actacatcca gtgttaaagg
    10021 acctgtctgg ccagttgctg gattttccgg atctgctggc tctactggtt ttgaaggatc
    10081 agtagggtta agaggctcca cggtatttgt tggttcagta aaggtaatat ctccttttga
    10141 agtcgcttga ccctcagcaa aagctggtgt tgccaagccg cctagaagaa gtgttgaaag
    10201 taataatgtc ccaatcatgt tctttttcat aataaatcac tcctttttct tagtgaccta
    10261 agtatagtag agacagtatg caaaaaatcc tcatgaattc atccgcttac ttgaaattat
    10321 gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt
    10381 ttttattaag tttcgtttgt caaattaagt tagacaaatc atcttttgtg acgtaactca
    10441 aaaatacttc cattggtgtc cgatagtgta atgattttct aggaatatga tttcgtttcg
    10501 atgccaccga ttggataaag gtttcagata caccattaaa atccatagct ttatacaaac
    10561 catctttacg taataagcca tttgaatgtt cattcaatcc tcgttgagaa ggtgttcctg
    10621 gatccgcaaa ataaattgaa atatcgttgg tattactaat ctttttccaa tttgagaatt
    10681 cttttccaca atcaaatgtg atggatttaa ataagttttt tgggacacaa cgaaaccatt
    10741 gattcagcct attttcaatg tctttagcct gtctgccttc gggtttcaag gtaatgatga
    10801 ctttagatag ccgttcaact aatgtgatga ccgcgctttt atgttttcca cccacgatag
    10861 tgtcaccttc caggtgacca aattcatgat caaactgggt atagtctgtc tttctgtctc
    10921 ggattgaccg gcgaaaccct tgttttcctc ttttttcttt atgaccgttt ggttttcttt
    10981 ttcccttcat tggtaaatcg tgagtgtcaa aggttttatc tttaaacatt cgatacagtg
    11041 tgcgtaccga acaagcaata ggaaactctg ctcgaccaat aaggacatca ggagcccacc
    11101 cttgaacaac ctttttttga atgtattcct gttgctcatc aggtaaaaca atcggatgtc
    11161 ttccgcagtt acgtttgttt ttcttatatt gtttatagta gctcattgcg cttccaccag
    11221 atttaagaaa acgatagacc ttataaatag tttgtcgaga acgttgtaac agattggcta
    11281 cttttgaaac aggttgtgat tgattgaaat aagcttctat cattacgagt tcagttggtg
    11341 taagatggtt ataggtcatt tgtactcact ccttataatt ctttcgtcgg aactattttg
    11401 agtgtagcac aaatgacttt ttttagttgt ctagcttaat tttacaatcg gcgaagtatg
    11461 aaaaaatatt tcaattttat tttttcgttt caaattattc tattagtgac atccttgaac
    11521 tataaaaata ggagtacggc atcatctgcc gcactcctat ttttttcgtc atttgtttct
    11581 taaataaaac gattctcacg caattttatt tttttgatat cttctactgt ttgtgttcca
    11641 gctaattgca tgaccatttc caattctgtt ttaaagaaat caaacacttg atggactcct
    11701 gtacttcctc ctaaagacaa tccataaata acgggtctgc caatagcaac aagatctgca
    11761 cctgatgcga ttgctttgaa aacatgctgt ccgcgtcgta caccgctgtc aaacacgatt
    11821 ggtacacgtt tatctactgc ttctgccaca tattgcaacg aatcaaacgc tgctggacca
    11881 ccatctagtt gacggccacc atgattagat acccagattc cgccagcacc agattctaat
    11941 gaacgataga catcttcttc tgactggaca cctttcacat agactggcaa atcagaatga
    12001 gcagcaataa attctacatc ttttggacta agcttctgtt tggaagattt gtacacagca
    12061 tccatcgttt ggcctactcc tgattgataa gcctgaacga tcggcattgg cagtgggaag
    12121 gtaaacccat ttctgcggtc tgtttcacgg tttccgccaa ctgttgcatc cgctgtcaat
    12181 acgattgctt ttgctccatt acgcttggcc acttcaagaa tatccaaatt gatcccatca
    12241 tctttgctca tataaaattg aaaccattgc ggtgctttct ccccgcctgc ttctcttatc
    12301 tcttctaatg tacaagaagc gtaagaactt gctgtataga tcgtcccaaa atctgctaca
    12361 ccttttgcag aagctttctc cgccttcaca tgagctaagc catgagcagc aactggtgcc
    12421 atgatgattg gtgcagtcag cgtttctttg tcaaaatgtg tagttgtatc gggtaattct
    12481 acatctctta atacatgagg aataatcagc tgatgattga atgcccgttc attttcttga
    12541 taagtaaata agtcgccagc accactgctg atgtaaccat aaccaccagc tggaatcact
    12601 tgtgaagcgg ctatttctaa atcttccata ttgataaaat ccactattcc ttcatttgta
    12661 cctgcttgat acgttttttc catttcaatg ccttccctct ataaaaaatt gaatgaataa
    12721 tatgatagcg tttacacact ttatattact cttccgtatt gatacaggca aataatctgt
    12781 ttacatttca gtcattccag ttgttactac tctgaaagaa atgtttagaa cacaaataga
    12841 taaaaaacga tgggattttg acaaaagtcg tgtcaaagtc ccttttacga aatactgtgg
    12901 atgcctattg aaataagtgc gggttgtttc tttgtatttc aatcgtttct tgccattctt
    12961 cgtttaattt tggattaccg ttttcttcct tgaacgcata aggtcccaat ccaaaaagct
    13021 cagttagtat ttgattcacg attccatata gctcggtgtg atcggacaca tgattattga
    13081 agatcatctc attatcacca cgtttgaatc tcacattcgg gctctcatga tgaacctcta
    13141 tgcagttttc cccttttttt aactgacgga tttctgaaaa catctcataa atatcttctc
    13201 caataaaacc ttcagcaagc gccagactgt ccacaagacc gcctactgtc aactcagaga
    13261 ttatcgcttt gttggcacca agctttaatg tatagcttgt tgcttctact acgccgcatg
    13321 cattgcaacc accttctgat agttcgcttt ttctgatcaa ttgaacttct ttcattttct
    13381 gttcctcctc ttagttttct acttatgtat tttttatcac ctgacgtaag tgtattttat
    13441 cgtcacgatc acgtgattta ttcttcagag attctacctg aataattcat acttttaatt
    13501 aaaaccatgt gaaactgcct cacggcagct tacaattact ttttcatctt cacccgttaa
    13561 gtgatgaaaa aagaacccct ttctgctatg gttaaagtga ctaaaccaac caaaagaaag
    13621 gagttctttt catgactagc ttacacaaaa accaagtaaa attcaattca aatatcacta
    13681 tttctcatac aggtggtcaa ttatcgagtg attcgggtct cgtcctagtg aaagagctga
    13741 tgaacatctt tggtttttct gaactggcta agcaacacat tcacattgaa gacgaacgag
    13801 cgtattttac tcatgacaac ttatccatac ttgagcagtt cattatgcaa ttaattgctg
    13861 gttactcagc tgattctgca gctaatctcc tgagacaaga tcctgtgttt aaagctgttt
    13921 tagataggaa agaactcgct tctcaatctt cactttcccg atttttagat cgactatctg
    13981 aggagaatat ccatgaactt caagctttga accaagaact tatcgataaa gcacgcctca
    14041 tccgtaatga tacggaatta atcattgatt tagattcgac ccattcagat acatttggtc
    14101 accaagaaca aacgaattat aatacacact accaaaccta tggttaccat ccattggtag
    14161 cttttgacgg attgactggt gacttcttaa aagctgaact acgttcagga aatcaatata
    14221 catcaaaagg cgtaaaggag tttctcacac ctttattaga gcactataat cactctctac
    14281 caaacactga catcttggtt cgtggagaca gcgggttcgc tacacctggt gtgtatgatt
    14341 cgtgtgaatc aaaaaagagt cattatgtta ttcgactgaa gaataatcgt agactaggtc
    14401 aaatagctga gaaatcagta ctttatggcg ataatcaaaa gtgggaagaa cgagaagttc
    14461 agtacttctc gaccacttat caagcacaat cctggtcaca aagtcgtcgc gtgtgtatac
    14521 gctcaacacg tgaagcgggc gaactactct ttcgacatga atttatcgtg acgaatctat
    14581 cagaaaatgt ttctcctgat accatctttt ctatctatgc caaacgtggc acaatggaga
    14641 acttcattaa agaagcgaaa gctggctttt actttgacaa aacagacagt cctcgctttt
    14701 tggagaatca tgtccgaatg atgctcagct tgatagctta caacttagtc aacttcttaa
    14761 gaacgattgg ctttgaagaa gtccaaaagg gaatgaccat tcattctatt cgattgaagt
    14821 ttctgaaagt tgctgggaaa ttagtccaaa cgggtagacg agtctatctc aaattatcta
    14881 gttatcatgt gtatcagaat gaattctaca gggtctttgc tcgcctgagg cgagccagtc
    14941 aatggatcta atccaacaat ctaacgattt ttttactggg aagttatcca gggagaagta
    15001 tgctcaaaat gccttacacc caaaatttat tcctcaatat ttttgaacag tgaatgtttg
    15061 aagtaacttt ttagtcaaaa tcagttgata gggatattag tgatacatat ttcaataaaa
    15121 tgtgccaaag aattaaagct atgaattatt caggattcta tttatctatc taacatatcc
    15181 tttattcttc tacttttctt tctttagcat ataagagata cgcatagtaa tcaagcaagg
    15241 attgttgata taaaaagaac aaataccgct attttgattt cttttgactc tattactcat
    15301 ataaataaaa tttattatac ctgctctttt tgactatatc ctcatctgac tcctatactt
    15361 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggctaaact tattaaataa
    15421 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat acggaatcac
    15481 tggtatgctt ccaagtactg tccaaacatt ggaacaacaa tctgtccaag cttacggtca
    15541 atacttaagc aaacagaccg atttggaaaa acgtatcttc ttaatgaatc tattcaatac
    15601 gaaccggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
    15661 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccaca
    15721 agatgctgta tttctttcaa tcgacgaacc agaaaatatc aaagcaagtt tgaaaaacgc
    15781 agcagatgga cgcgacattc gtttgatcgt tgtgacagat gcagaaggaa tcttaggtat
    15841 gggtgactgg ggtgtgaacg gtgttgacat cgctatcggt aaattgatgg tctacactgc
    15901 cgcggctggt atcaatcctg cacaagtctt gcctgtatca atcgacgctg gtactaataa
    15961 cgaagaatta ttgaataatc cattatactt agggaatcgt catgctcgtg tagaaggcga
    16021 aacttactac gaattcattg atcaattcgt tcaatcagct actgaattat tcccagaatt
    16081 gttattacat tgggaagatt ttggacgagg aaatgctgca acgatcttag aaaaatacga
    16141 agataaaatc actactttca atgatgatat ccaaggcaca ggaatcgttg tactagctgg
    16201 tgtattaggt ggattgaata tatcaggtga gcaattgaaa gatcaaacga tcttgacatt
    16261 tggtgctggt actgctggtg taggtatcgc aaacatcctt ttagatgaaa tgatccgtca
    16321 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac agggtttatt
    16381 gtttgaagat acaaaaggat tgactcctgg tcaaatccca ttcgctcgta aacgctcaga
    16441 attcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcaa tccatccaac
    16501 gatcatgatc ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
    16561 ggcggctcat accccacgtc caatcatcat gcctttgtct aatccaacaa aattggcaga
    16621 agcaaaagca aaagatttga ttgaatggac ggacggaaaa gctttagttg gaacaggtat
    16681 ccctgctgct gatgtggaat ataacggtgt aacttaccaa atcggtcaag caaataatgc
    16741 gttgatgtac ccaggtttag ggcttggttt gattgcttca acagctacac gagtaaatgc
    16801 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
    16861 agcagctatc ttgcctccag tagcaaaaat cactgaattc tctcaaacga ttgctgaaac
    16921 tgtcgcaaaa tcagttgttg cacaaaaatt gaatcgtgaa gaaatcacgg atatcaaaga
    16981 agctgttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagattgtta
    17041 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
    17101 aaacagcagc cagccaacta ctgctctttt gccgcttctc tttggctttt ttcatacgat
    17161 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaaccgcaa ctgcagttga
    17221 ggaaaagaaa aaagaagggt tttggtcaac aaaaattacc ggcgtaagtt tgccccttta
    17281 tttgatcatg gtccttgtcc tgatcattgc aatggcttta ggaaaattac cgacgaatat
    17341 gattggccca atcgctgttt tggtcatttt cggtaattta tttcatttta tcggaacaaa
    17401 aatccctata gtcaaaagtt atttaggtgg tggctccgtt tttgcgatct ttgcctctgc
    17461 cgcgattgct acatttggga tcttacctga gtatgtagta aaatccacag aaaactttgt
    17521 cagcaatatg ggctttttag acttttatat tgctgccttg attactggta gcatccttgg
    17581 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
    17641 aatggtcgtc tccttttttg ccgtaggact cgtaggtatg cttatcggaa atggttttgc
    17701 aaattccgtt ttatatatct cattgcccgc aatggctggt ggtgttggag ccggagctgt
    17761 acctttatca acgatttatg caaatgtttt aggtacggac gcttcttcta tcatttcccg
    17821 cctgattcct gcgacagcaa tgacaaatgt cctagctatc attggtgcag cgttagttgc
    17881 tcgtgcggga caatcaatgc caaaattcaa cggtaatggt aaattaatgg aaaaaggcga
    17941 ccttggcgat ggaaaaccta gaaatgtaaa accttccatt ccacaattag gtgtcggttt
    18001 gatggtatca ttgactttct tcatcttagg acaaatctgt aactattttg ttcctaaagt
    18061 ccatgcttac gcattcatga tcattatcgt tgttatctgt aaaatcgcaa acctattacc
    18121 agaatattac gaagatgcag ctatcatgtt caacaacttg attgtcaaaa acttgacagc
    18181 agctgttctt gccggtatcg gtattgcgtt attgaatcta aatgtattgg cttctgcttt
    18241 gacatggcaa tttgttgtct tatgtttgac aagtgttatc gcgatttcag tagtttcagg
    18301 atttgtcggt cgtttgtttg gtttatatcc aatcgaatca actattactg ctggcttatg
    18361 caacaactcg atgggtggta caggaaatgt agctgtctta tctgcagcta atcggatgga
    18421 acttattgct tttgctcaaa tgggcaatcg gcttggtggt gccatcgttt tgatcatctc
    18481 aggtttcttg atgcaattac tttcttagtc aaaaaaaata cctgatcatc aagtatcggc
    18541 ttgatgacca ggtatttttt gataaaaatt tacaggattt tcaaaaatct tttttgcatt
    18601 ttatcggaat gataaccgat gcgttcataa aatcggtgtg cttctactct gtattctgca
    18661 gaattaagtc ttattgctga tattcctcgt cttttcgctt cttctacgcc agtcatcaag
    18721 gcctttccga ttcctctttt ttcagcatgt ttagacacag caagtgccat aatattgaac
    18781 atcggatcgc tgaaaatcga ttcataggac tccgcttgta catagccagc tgctttttta
    18841 gaagtaggat cttcatagac caagaaaaaa tttttttctg gctccttcaa cagtttcttc
    18901 atttgtctca ccgtaacggt taatggtaca tcatatccta attgttccgc attgatttct
    18961 cgtagtgctt ctgcatctcc taaacgcatt tcccgcaaca tccaaatcac cagccaattt
    19021 ttattttttt aaatgaattt ttttatcaca gtatactcct tttttcactt aaaaaaagac
    19081 ttttcagtga ttttgacttg cttgaatcca gatccttcta atgtttgcaa gatgacgaaa
    19141 gcattgtaaa gattgatcaa aagaaccacg atgacaatcg cttgaaaaat aaaggtcact
    19201 ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat aacagcagcg
    19261 gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt taaagcaatc
    19321 gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag tgttgcttct
    19381 tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc accaccaata
    19441 cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca agtccatgac
    19501 gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac caaggtcaat
    19561 accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa ctgtaaaaaa
    19621 acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga tacccaaccg
    19681 acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga aacaatggac
    19741 atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg ggcgccgata
    19801 agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc gccaactgtg
    19861 ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt cctttctctt
    19921 gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa gaaacaaacg
    19981 aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa aaaccgggct
    20041 ctacttttca gagagtccag cttttcttac accttatcga ttcttttgtt ctattcttct
    20101 tccgccatga atgtattgaa tacttcttcg atcatatccc attctgcgtc tgtttcgatt
    20161 tgttctaact cgccttctgt tccatcttcg ttttcgatgt atgcataagc ttgtaattct
    20221 acatcttcat cttctgaaac accagctgga tataaaagaa catagttacg tccaaattct
    20281 tcttgtccgt caactgttag caagatttcg tataatgttt catttccttg gtcatccacc
    20341 aatgtgatgt gttcgtgtcc ttcgtgttcg tgggaatggt tatgttctgt cataagatta
    20401 cctcttttct ttttattatt aaaataattg cttacctgag ccgtctaaat aattttgtaa
    20461 aatcatcaca gctgctaatt tatcgatgac tttttttcgt ttcgctcttg aagtatttgc
    20521 ctgctctact agcatacgtt ctgcctgaac agtggttagt cgttcatctt gataatcaac
    20581 cggcaaggag aatagttctt tgatcttctc tccatatgac atggaagctt ctgcccttgg
    20641 tccgatggaa ttgttcatat tcttaggtaa tccaactaca aaacggtcta cttcgtactg
    20701 cgccactaat tccttcaacc gatcaaaacc aaactcttct tctgcctcat tgattcgaat
    20761 gatttctact ccttgagctg tccagccaaa gggatcgctc acagcaacgc ctactgtctt
    20821 agaaccgaca tccaagccca ttactctcat aaattcactc catgatttga gaggtagtct
    20881 ttgacaatta cttccatgat ttcatcgcgt tcgtgacggc gaatcaaatt tcgtgcatca
    20941 cgataacgag gaatgtaggc cgggtctcca gaaagcaagt agccgacaat ctgattgatt
    21001 ggattgtagc ctttttcttc caaagcccga taaacaatcg ctaatgtttc gctcacttct
    21061 tttttgcgac tatcgtcaaa accaaaacgt acggtttcat ctgtaaaacc catgattcta
    21121 cacctctttc ccgcaaggta attccagctt accttctaca cccttatttt actcgaagaa
    21181 agggaaaact acaaacaaat attccaaact tctgaataaa ttaagaaaca cgatttgtct
    21241 gatttttcct tttttctgtg gctttttcaa aagccctctt tccataaagt atacgctata
    21301 tttctttgaa agtatgtttt tacacaattt tttcataaag ttcataaaaa attcgaattg
    21361 ctgctactaa tttttcgttt tcgttgcttg ccctgtctca taattgatgg tgtagccatc
    21421 ttgaacattg tagataaaga cattgaattc aatttttttg tcttcaatgc tttctgcttc
    21481 caactgcaca cctcttgcta ccagttcatt tccctcgaaa tgaggagtga cacgatagcg
    21541 tacatgatga tttgtttcct tgatatatgc cgcaatatca ttttcgtggg caagcatgta
    21601 cggagcattc aacgaacgtg tccctgtcat taagttttta atgttattat tttgaccagt
    21661 caactggaaa ccaatcaaat ggctgcggtt gtataaccat tgtccgtcac ttaatttctt
    21721 ttgtttccaa cctgtcggat caatatacaa aggttctctc ttttcagtag gaaacaagtc
    21781 ttttcccaac atcgcattgg cttgtcctac tctgttcaat ttgtctatat ttgaatagct
    21841 ttcccatgtc cctttggaaa gactaagatc ctcttttgta aactccggct gattcccatt
    21901 gatttctatt tcttgatgtt ttccatcata ttctggcaca tccgctactt gagaagcttg
    21961 cggtgtggaa tcaaaaatgg ttgtaatttg ttggaagata tctgaatctt tttgtgtggt
    22021 ggaaataccg agtgcccctg caatcacagc agcgacgatc cccacgatcg ccttggttgt
    22081 ttttttgctc atttctttac tcctttacta acagttccct aaatcttaac atatgttccc
    22141 ctataaataa atactggaaa gaaatagaaa cagtaaaaaa ataaaaccag tttttagacg
    22201 gcttaagaat acaaaaaaag agacggattc atcgtctcta ctcaatcaca aaacactatt
    22261 tacaaggtat ttattatcat tcaaaaaatt tttcttaata ctcaaaacta aacggcttct
    22321 gtacgacctc ttggtaaaca gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    22381 gtcaaaaaca taagaagctt ttttgcttga ctattcttat tacttgatgc agggcagctt
    22441 tttcacagcc tcttttaagc gggtgacggg aatcgaaccc gcgacatcag cttgggaagc
    22501 tgggatttta ccactaaact acacccgcga tacaaaaaca gtatacgcct ctttcactcc
    22561 ttcctcaagt ttttttattc tttacgcttc cgatatgttt tgtagtaaaa taaaactaat
    22621 caatcaatta ttggagaaga tgctatgacg ggaaaagaat tagatctgtt gctggacact
    22681 tgtttactcg ctgggaaaat catgatggaa agcaatgcgg agatgtatcg ggtagaagat
    22741 acgatgagtc gaattgcttt agcttctgga aatttccgtt tggtcagcta tgtcactcaa
    22801 accggtctct ttattggtct tgatcgtacc tcgaccatcc gaatggaaca aattaccaac
    22861 cgttcgatta atttagaaaa agtagtaaaa gtaaatgatc tttcacgtaa atatgttgct
    22921 tcagagctaa cattagaaga actttactgc gaactgcaag caatcgaccg agaccgttct
    22981 ttctttcctg tctggttgca aatcgttagc gcggcagtga tcagtggaac aattatggtg
    23041 ttatttggcg gaaagatgac cgatttacct gtgactttag tcattggagg attgggatac
    23101 gcagcttatc tatatagttt gaaatttttt agaatcaaat ttttatcgga atttttatct
    23161 tctttattga ttggcatagc cgctatctac agcgtacgtt ttggcttagg aagcaatcag
    23221 gatctaatca ttatcggatg tgtgatgcct ttagttcctg gagttcaaat tacgaacgct
    23281 atccgtgatt tgctagctgg acattatgtc tcaggagttt ctcgcggaac agaagcgatg
    23341 atgactgcta cgatgatcgg ttttgcgatt gcttttattt tccagctttt ttattaggaa
    23401 aggaacacaa gtatggggac gatatttatt cagttttctt ttagtttttt agcgactgcg
    23461 gcatttgccg ttattacaaa tgtacctagg cgttcattga tttgttgtgg tctttctggt
    23521 gcttttggct ggatgatcta ctggatctgt gttcaaatag gcggatctgc tgcttttggt
    23581 tcattgcttg gttctttagg agtagcattc atcagcgatc ttttctctaa acgtttgaaa
    23641 atgcccgtaa cgattttcaa tattcccggg atggttcctc tagtccctgg tggtttagct
    23701 tatcaagcgg ttcgaaatct cgttactgaa tcctatcgag aagcggctca ttatgcggtc
    23761 caagcaatta tgattgctgg agcaatcgct ttaggtcttg tattatcaga agtctttaac
    23821 cataacatcc gtaatttcaa agtaaagcgt gacgcaatca tgatgaaacg aaaaaaacgg
    23881 ctttttaaat aaacgaagtg taacaaagaa aaggttgtga aagagacgtt cagccttgag
    23941 cgatgacaaa atatctcaca aacattgcga caagtaaccg caggagcaat aagaagtaat
    24001 tatgagtatt aaaaaaagtt ttgtaaaaat agcttctcac atttttacat aaaattgact
    24061 taatactgag aaactgactt ttgaacaccc tctatctgta aaaaaggggc tgcgacatga
    24121 tttttgtcac ggcccctctt ctttattcaa attttttcca aaacgttcgt tgataataaa
    24181 cgatccatag agcggaaagt attagaacta aaccgatcac aggaatattc actaaccaaa
    24241 atggataaaa caaagccaac attccgtata agacgataag aataatactg atgatcaatg
    24301 aacccatcat agttgcgact aaacccaaac ttcctgaacc tctgttaaat aattggttaa
    24361 tattggtcca gttcagcagc agcaaccgat aatctctagc gaaataacgt agtgacaata
    24421 agtagcagcc gaataaagaa ccaaatagca agctagggat aaaaaacaaa ggcagctgaa
    24481 aagacagtcc gcttaataat gcgatcccac cagttaaaaa actctgcaat atccatccga
    24541 atctgaactt ttcttttaaa tattggttca tagagattgg caaagaacgg ataaacagaa
    24601 aattttcttg atccaaagaa ataagattgc tgataaaaga agtctgatta actgttaaga
    24661 aagccaatgc gatccctacc aggaaaacaa caccgattaa cttattatct aaatcagtta
    24721 attttatttc gcctcctagg gcaaaagcaa gaataaaaat gattggagtt aacaaggaag
    24781 aagaaagaac ttgcataatc aaattaggtt cttttactaa ttgagcattg taactaaata
    24841 aaagctgatg cacattttgg ttctttttat gggttctttt tattttgaac tcactgtttg
    24901 aatcagtcag ttgctctgta agctttggta ctatcagctg ttttacagca aggaaaaatc
    24961 ccagagccgt gatagctagt attccccaac ccaataaacc ttgttgagaa agtggctgac
    25021 tggcagcatc atagaatggc aataatatcg aaatcggatg ccgatctgtt aactcatttt
    25081 caatagtccc cgtttgatga ttcatccaca tgattccagc aaatgcgatc agcatcgagc
    25141 tgccaagcaa gatactagaa actgtttttc tatgtttttt aaacactgct gttcgagcca
    25201 aaccaaaaac aatcaaacta gccagacaaa aaagaataat caaaaataga gaaaacaaaa
    25261 ggattgagat aatgatagta agcaaaatcc ctagatcagc tcgccatccg gttaaaataa
    25321 acatgactag aagcggaaaa acaaaaggaa tgatggtcaa agcaactacc aaaatctttg
    25381 ctgaaaaaac ctgttcttgt ccaaaaggca gaggcaaaaa tacttgcagg tcatttcctt
    25441 caaaaaagat attataaatg actgatatac tttgagttaa acctaacata ccaaaaagtg
    25501 ccacgtaata agtaaagaaa cctggcaatt taacaaagtt catagcaaac atagcaaatc
    25561 cgtaaatcag taaaaaaatc agccctgaaa aaagattttg agacaaaaga tagatagtta
    25621 atcgctttcc cttttttcct ttctctcttg ctttattcgt gagctgtgga ttaacatata
    25681 aaagattaac tcgaattaat tctaataatt gtcgcttata catacggtcc atctcctgtc
    25741 gcttgccttc cagccatttt caaataaatc ttttctagcg tttcatcagg tgagccattc
    25801 aataattcac taactgcccc ctgataaatc aattcacctt tcttcaaaat agccagttta
    25861 tcgcataact gttgtgcggt atccaataca tgtgtagaaa aaatgactgt ttttcctttt
    25921 gcggcatgag ccttcatcat ttctttcaaa tcaaatgctg cttgaggatc aagtccttgc
    25981 aaaggttcat ccaataccca aatatctgga tctggcagca acgtgccgat caaaatcgtt
    26041 ttttgacgca ttccatgtga aaaacttgcg attgtttcat tttgatgact atacatatca
    26101 aaaagcgctg tcaattctgc taaacgcttt tctttttttt gtgtgtctaa ttcataggca
    26161 gctgaaatca agtcccaata ttctccagcc gtcaattgta aaaaaatatc tggggaatca
    26221 ggcacataac cgattttttg tttgatactc aatcgatttt ccgtcaattt cattccatct
    26281 actaaaatcg ttcccgatga tggttcaata atactaacta aacttttaat cgtagttgat
    26341 tttccggcac cattatgtcc taaaaatcca aaaatttccc cttgtcttat cgagagagac
    26401 aagtctttta atgcttgttt tttcccgtaa cttttactta cattgatgaa ttcaatcatt
    26461 tctgtactcc ttttgtttgt tattactttt tattgtaccc ttcgttattg gtggagtaaa
    26521 tgtcaaaaag agtgcataac aaaaaaggat agaaacgact aggctgctac ttgggttgtt
    26581 ctcttctaca ggaaaacact cttctaacag gtaagatatg agtctttgtt ttctttaaaa
    26641 tgattcattt tctatctttt cttgcataaa attctttagt ttatcaatac ttctctttcc
    26701 catctctagt tgttctccat ttgcaaatgt tactatttcg tttactctgc taatagatgt
    26761 aatgttttca gtatt
//
LOCUS       DASJRQ010000034        26691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJRQ010000034 DASJRQ010000000
VERSION     DASJRQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.034"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="U4A80_001260"
     CDS             complement(104..1336)
                     /locus_tag="U4A80_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1974891.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="U4A80_001261"
     CDS             1591..2160
                     /locus_tag="U4A80_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1974892.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="U4A80_001262"
     CDS             complement(2338..2778)
                     /locus_tag="U4A80_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1974893.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="U4A80_001263"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="U4A80_001263"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1974894.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="U4A80_001264"
     CDS             3732..4655
                     /locus_tag="U4A80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1974895.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="U4A80_001265"
     CDS             4731..5870
                     /locus_tag="U4A80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1974896.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="U4A80_001266"
     CDS             5863..6663
                     /locus_tag="U4A80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1974897.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="U4A80_001267"
     CDS             6663..7490
                     /locus_tag="U4A80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1974898.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="U4A80_001268"
     CDS             7468..8202
                     /locus_tag="U4A80_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1974899.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="U4A80_001269"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="U4A80_001269"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1974900.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="U4A80_001270"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="U4A80_001270"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1974901.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="U4A80_001271"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="U4A80_001271"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1974902.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="U4A80_001272"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="U4A80_001272"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1974903.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="U4A80_001273"
     CDS             11787..13820
                     /locus_tag="U4A80_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974904.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="U4A80_001274"
     CDS             13864..15144
                     /locus_tag="U4A80_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1974905.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="U4A80_001275"
     CDS             15354..16160
                     /locus_tag="U4A80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974906.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="U4A80_001276"
     CDS             16172..17392
                     /locus_tag="U4A80_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974907.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="U4A80_001277"
     CDS             17382..18968
                     /locus_tag="U4A80_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1974908.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="U4A80_001278"
     CDS             19007..21145
                     /locus_tag="U4A80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1974909.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="U4A80_001279"
     CDS             complement(21513..22496)
                     /locus_tag="U4A80_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1974910.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="U4A80_001280"
     CDS             22874..24265
                     /locus_tag="U4A80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1974911.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="U4A80_001281"
     CDS             24281..25321
                     /locus_tag="U4A80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1974912.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="U4A80_001282"
     CDS             25340..26425
                     /locus_tag="U4A80_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1974913.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26691
                     /locus_tag="U4A80_001283"
     CDS             26458..>26691
                     /locus_tag="U4A80_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1974914.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLV"
BASE COUNT         8550 a         4443 c         5425 g         8273 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt a
//
LOCUS       DASJRQ010000035        26370 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJRQ010000035 DASJRQ010000000
VERSION     DASJRQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.035"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..611)
                     /locus_tag="U4A80_001284"
     CDS             complement(243..611)
                     /locus_tag="U4A80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1974915.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="U4A80_001285"
     CDS             761..1243
                     /locus_tag="U4A80_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1974916.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="U4A80_001286"
     CDS             1280..2290
                     /locus_tag="U4A80_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1974917.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="U4A80_001287"
     CDS             complement(2405..3118)
                     /locus_tag="U4A80_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974918.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3308..3898)
                     /locus_tag="U4A80_001288"
     CDS             complement(3308..3898)
                     /locus_tag="U4A80_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1974919.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4382..4609
                     /locus_tag="U4A80_001289"
     CDS             4382..4609
                     /locus_tag="U4A80_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1974920.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4625..5791
                     /locus_tag="U4A80_001290"
     CDS             4625..5791
                     /locus_tag="U4A80_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN1974921.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6025..7068
                     /gene="hrcA"
                     /locus_tag="U4A80_001291"
     CDS             6025..7068
                     /gene="hrcA"
                     /locus_tag="U4A80_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1974922.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7162..7725
                     /gene="grpE"
                     /locus_tag="U4A80_001292"
     CDS             7162..7725
                     /gene="grpE"
                     /locus_tag="U4A80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1974923.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7776..9605
                     /gene="dnaK"
                     /locus_tag="U4A80_001293"
     CDS             7776..9605
                     /gene="dnaK"
                     /locus_tag="U4A80_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1974924.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9756..10922
                     /gene="dnaJ"
                     /locus_tag="U4A80_001294"
     CDS             9756..10922
                     /gene="dnaJ"
                     /locus_tag="U4A80_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1974925.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11004..11669)
                     /locus_tag="U4A80_001295"
     CDS             complement(11004..11669)
                     /locus_tag="U4A80_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1974926.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11645..11818
                     /locus_tag="U4A80_001296"
     CDS             11645..11818
                     /locus_tag="U4A80_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974927.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11863..12600)
                     /locus_tag="U4A80_001297"
     CDS             complement(11863..12600)
                     /locus_tag="U4A80_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1974928.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12602..14104)
                     /locus_tag="U4A80_001298"
     CDS             complement(12602..14104)
                     /locus_tag="U4A80_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1974929.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14363..16357
                     /gene="uvrB"
                     /locus_tag="U4A80_001299"
     CDS             14363..16357
                     /gene="uvrB"
                     /locus_tag="U4A80_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1974930.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16367..19183
                     /gene="uvrA"
                     /locus_tag="U4A80_001300"
     CDS             16367..19183
                     /gene="uvrA"
                     /locus_tag="U4A80_001300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1974931.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19446..20711
                     /locus_tag="U4A80_001301"
     CDS             19446..20711
                     /locus_tag="U4A80_001301"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1974932.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20715..21446
                     /locus_tag="U4A80_001302"
     CDS             20715..21446
                     /locus_tag="U4A80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1974933.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21581..22465
                     /gene="rapZ"
                     /locus_tag="U4A80_001303"
     CDS             21581..22465
                     /gene="rapZ"
                     /locus_tag="U4A80_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1974934.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22462..23460
                     /locus_tag="U4A80_001304"
     CDS             22462..23460
                     /locus_tag="U4A80_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1974935.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23494..24429
                     /gene="whiA"
                     /locus_tag="U4A80_001305"
     CDS             23494..24429
                     /gene="whiA"
                     /locus_tag="U4A80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1974936.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24972..25622
                     /locus_tag="U4A80_001306"
     CDS             24972..25622
                     /locus_tag="U4A80_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974937.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25809..>26370
                     /locus_tag="U4A80_001307"
     CDS             25809..>26370
                     /locus_tag="U4A80_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN1974938.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8708 a         4450 c         5602 g         7610 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca
     1921 tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag
     2341 aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga
     2401 aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg
     2461 taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt
     3241 gacaaataat catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag
     3301 agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt
     3361 atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca
     3421 agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag
     3481 cttgttgttc tataaaatct tctttattct gataattttc cgattctaac caaggaaatt
     3541 gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg
     3601 ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata
     3661 aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt
     3721 caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct
     3781 ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa
     3841 tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt
     3901 tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag acattcatta
     3961 aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag
     4021 tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca
     4081 ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat
     4141 attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc
     4201 actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa
     4261 aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta
     4321 gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc
     4381 tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc
     4441 gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc
     4501 atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct
     4561 tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat
     4621 ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc
     4681 attttgcgag catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc
     4741 agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc
     4801 gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa
     4861 acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga
     4921 gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa
     4981 ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa
     5041 aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga
     5101 aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg
     5161 atatctggat actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc
     5221 attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact
     5281 gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca
     5341 tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa
     5401 cctcatgtac tggaacaatg cccactattt tgggtttgga gctggtgcta gcggttatct
     5461 tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc
     5521 aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga
     5581 aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta
     5641 tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt
     5701 acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa
     5761 tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat
     5821 ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat
     5881 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
     5941 ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact tagaatatat
     6001 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
     6061 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag
     6121 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
     6181 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
     6241 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
     6301 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
     6361 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
     6421 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
     6481 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
     6541 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
     6601 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa
     6661 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc
     6721 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
     6781 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
     6841 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
     6901 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
     6961 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
     7021 gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga
     7081 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
     7141 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
     7201 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
     7261 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
     7321 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
     7381 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
     7441 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
     7501 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
     7561 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
     7621 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
     7681 catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa
     7741 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
     7801 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
     7861 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
     7921 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
     7981 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
     8041 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
     8101 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
     8161 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
     8221 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
     8281 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
     8341 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
     8401 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
     8461 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
     8521 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
     8581 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
     8641 taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta
     8701 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
     8761 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
     8821 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
     8881 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
     8941 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
     9001 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
     9061 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
     9121 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
     9181 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag
     9241 atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
     9301 atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
     9361 acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag
     9421 caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
     9481 tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag
     9541 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
     9601 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
     9661 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
     9721 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
     9781 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
     9841 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
     9901 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat
     9961 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
    10021 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
    10081 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
    10141 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
    10201 atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa
    10261 ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg
    10321 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag
    10381 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
    10441 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
    10501 gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata
    10561 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
    10621 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
    10681 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
    10741 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
    10801 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
    10861 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
    10921 agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg
    10981 ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata
    11041 tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga
    11101 tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta
    11161 tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt
    11221 ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc
    11281 ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat
    11341 tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc
    11401 gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta
    11461 tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt
    11521 ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat
    11581 aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca
    11641 taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga
    11701 tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt
    11761 ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa
    11821 atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt
    11881 attcaagaaa tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc
    11941 atcttctaaa aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa
    12001 ccccatttcg tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac
    12061 gttcaagaca tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat
    12121 gatgtcaggg ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga
    12181 aagcatttca ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat
    12241 tgcttttttc tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat
    12301 gttttccaaa atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg
    12361 ttgtctcacc aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat
    12421 ttcaccgtca gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga
    12481 acctgaaggg ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt
    12541 taatactgtg ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat
    12601 cttacttcac cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca
    12661 agtacatgat cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga
    12721 tcttaccagt ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta
    12781 atgagataac gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa
    12841 tgattttttg cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc
    12901 cgacaggaac ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa
    12961 gagtcaaggt gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca
    13021 acccaaagaa aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat
    13081 aaatactagc aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca
    13141 caccgattac tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc
    13201 ctttcaacaa tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag
    13261 tggaagactc actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt
    13321 attcgcctgt tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat
    13381 tttgtccttt tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt
    13441 ccaattcttg tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat
    13501 cgattgcgcc aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag
    13561 aatagccata ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg
    13621 ctccgactgt ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga
    13681 cagctaattg gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt
    13741 catctgttac agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca
    13801 aagcagcaga gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt
    13861 tttccatcaa ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg
    13921 gagcaaaagc agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca
    13981 ttgtctcgct tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa
    14041 acgcaagtgt cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt
    14101 tcataatcaa cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca
    14161 gacaagactg taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata
    14221 aaacataaca tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt
    14281 ttttagtaag gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa
    14341 tgaatgaaaa ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt
    14401 ctcgaaatac caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt
    14461 agtcggcggg aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac
    14521 gatttccaat cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac
    14581 attggctggt cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata
    14641 ttttgtttct tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta
    14701 tatcgaaaaa gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag
    14761 ttcattatta gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt
    14821 aggctcgcct tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga
    14881 tcgcaatcag ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt
    14941 tcagcgcggt cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga
    15001 tgaacgtgcc ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa
    15061 tgcactgaca ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca
    15121 ctttgtgacg aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga
    15181 acagcgttta actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca
    15241 gcgaaccaat tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa
    15301 ttattctcgg catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt
    15361 ctttcctgaa gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag
    15421 aggaatgtat aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt
    15481 gccatctgct ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca
    15541 gatcatttat gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat
    15601 ccaacaaatc atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat
    15661 gggacaaatc gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt
    15721 gtttgtaacg acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt
    15781 agggatcaaa gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat
    15841 tcgagatctt cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg
    15901 attagacgta cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt
    15961 acgtagtgaa cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa
    16021 agtaatcatg tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc
    16081 aagacgtcgt acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat
    16141 cattaaagaa atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga
    16201 agcggtacaa gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa
    16261 attagaaaaa gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt
    16321 gcgagatatg attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa
    16381 aattacgatt catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg
    16441 agataaattc gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga
    16501 tacgctgtac gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca
    16561 gtttttagga caaatggata agccggacgt agatagcatc gacggattga gtccggcgat
    16621 ttctattgat cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga
    16681 aatcaatgac tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga
    16741 tcatatcgag attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc
    16801 tgagcgcaca aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa
    16861 aaaaatattc gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat
    16921 ctatgattta agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt
    16981 catcgaccgg atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc
    17041 ggccctgcga ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt
    17101 gtttagcgaa cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag
    17161 actgttctca ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa
    17221 attagaagtc gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc
    17281 tatcgttcca tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg
    17341 tgaaagtttc ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga
    17401 gatcatcttg aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg
    17461 cgttcgcgat gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca
    17521 tgatacgaac agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg
    17581 tcagacatgt aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac
    17641 gaatatcggg caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt
    17701 aacgttatct gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag
    17761 actttcattc ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac
    17821 attatctggg ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc
    17881 tggagttttg tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg
    17941 actgattggc tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca
    18001 tgatgaagat acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga
    18061 acaaggcgga caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc
    18121 tttgacaggg aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa
    18181 aggtaatggg aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag
    18241 tgttgaattt ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag
    18301 tacattggtc aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc
    18361 taagccaggt aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat
    18421 cgatcaaagc ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt
    18481 tgatgatatc cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa
    18541 agggcgtttt agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat
    18601 tattaagatc gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg
    18661 taaacgatat aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat
    18721 tttggaaatg acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg
    18781 caaactgcaa acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac
    18841 gacattgtcc ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc
    18901 taatggaaag aatttctata tactagatga accaacgaca ggacttcata gcgatgacat
    18961 cgcccgcttg ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat
    19021 tgaacacaat ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg
    19081 tggagaaggt ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa
    19141 agaaagttat acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga
    19201 gatagttaaa aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta
    19261 gaatcattac atggtagttc tttctacact gattggtata gataaaaact tcctataagt
    19321 tcaatccgtt caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt
    19381 tatggtatga tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag
    19441 agataatgat gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca
    19501 tgaacgttta tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat
    19561 atgcttcgga tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc
    19621 caggatttga taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat
    19681 attctgataa gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga
    19741 tctcccaaca taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt
    19801 tatttgaaag gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc
    19861 cgtatcctaa aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg
    19921 aattgccttt tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg
    19981 tccaatttga aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa
    20041 ttttaggccg gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc
    20101 cgggtgatga tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc
    20161 ggatgatgtg ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt
    20221 acgttgtgat catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg
    20281 aaaaaatcga atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag
    20341 aatataaatt atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga
    20401 acggcttgaa tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg
    20461 aaacgaccta tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa
    20521 aaatcttttt agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca
    20581 aagaaaatcg ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt
    20641 tgttgatgaa atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga
    20701 aagaaggcta gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga
    20761 cagaaagttt tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt
    20821 tgaattatgt cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc
    20881 gatcagaaga aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc
    20941 gaccaaactt cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag
    21001 cggcaacgga tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc
    21061 agcatacgac agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct
    21121 atgaacgcga agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag
    21181 aaaaaatcaa ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc
    21241 tctattacga aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag
    21301 gttgcacaga attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg
    21361 atgctcaatc tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg
    21421 cattggatac agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat
    21481 atttggtcca aatctttttg taaaagaaaa agaaaactgg taatgatacg cccgggatga
    21541 tataatttta ttatgcacaa tgtgagaggg gttcgtaaat atgacagata atttgcaatt
    21601 agtcatcata acaggtatga gtggtgcagg gaaaacagta gctatccaaa gttttgaaga
    21661 tatgggctat ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt
    21721 gatcaaagaa tcaggtaaag tcacaaaaat ggctttagga gtggatttgc gttctcgttc
    21781 cttctttgaa gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac
    21841 ttctatctta tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag
    21901 acgagcacat ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc
    21961 gatactagac gacttgaaag cccaagcttc cgtgatcatt gatacgacaa cattgacacc
    22021 acgacaatta cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg
    22081 gatcgaattt gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat
    22141 ggatgtgcgt tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa
    22201 agatcagcca gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa
    22261 atttttggat cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt
    22321 gacagttgca atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt
    22381 ggcaaatgct ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag
    22441 gaaagagacg gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc
    22501 gtagtggtcg gcggtggcac tggtctgcca gttatcctga aaagtttacg aaatcaagga
    22561 gcagatatca cagcagtagt gactgttgca gatgatggtg gaagcagcgg tgcaatcaga
    22621 gaatcgatcg ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat
    22681 atgccacaat tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta
    22741 gccaatcata cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact
    22801 tatgaggcga ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct
    22861 tcagaaactc ctttgacctt gcatgctgtt tttaaagacg gaacgactgc agtaggagaa
    22921 tctaaaatcg cgatcaaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat
    22981 aaaagtgaag ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc
    23041 gttttgggac ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc
    23101 ggcgaagcaa tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa
    23161 aaaggggaaa cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg
    23221 aatcagcctt ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg
    23281 gacccagaga tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga
    23341 aatgaaggat gtcgagtgat ttccaccgac tttttagaat tgaaagatgg tggtgtcttc
    23401 cacgatggag acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa
    23461 gcaatttatt gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa
    23521 gaactaactg gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg
    23581 cgaatgaacg gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa
    23641 aatgctgcaa ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt
    23701 gagctactcg ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg
    23761 aagcaaggca caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat
    23821 ggacacgttt cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt
    23881 gcttttatgg cctcaggttc agtcaataat ccggaaacga gtcgttatca tttggaaata
    23941 ttttctattt atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta
    24001 catgctcgaa cgcttgaacg gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa
    24061 attgctgatt tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt
    24121 cgaatcgtac gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat
    24181 ctgaacaaga cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca
    24241 aaggtcggtc tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa
    24301 catccagagg tcagcttgaa agaattaggg gaaatgattc cctcaggtgc aatatcaaaa
    24361 tcgggaatta atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa
    24421 gcagtttaat ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt
    24481 ttttaaaagc tatacttgct tttcttccgt caaactataa ttccaacttt actggagtaa
    24541 cataatgttg aaaggcttct attgtttttt aatacaatcg tataaaaagc ggggagacaa
    24601 ctgaatgaag attttagttt tatacataaa cttttgctta atatagaagt cctgatatag
    24661 ataggatcaa gtatttatct ataactggag attaattaaa aactggaagg aactgctcga
    24721 tcaaccttga gaactgtttt tcttacttct ggtcaagatt ctcgtggttg gtggataagg
    24781 atggttgata agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa
    24841 gttttaacaa ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat
    24901 tgggatgtat ggttggtata cgttatacct gaacttgaac tatcaagatt tggtttagaa
    24961 tttgagaatc gatgtttata aagggagggc aaaaaatgtg cttttttaca acttttacta
    25021 tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt
    25081 tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt
    25141 ttctattcac agttattgta tttatttgga gaactacagc accaccattg ccagagatta
    25201 catatggaga atttccgttt agtcttgaat atgaaataga tgaggaaaga attttgattg
    25261 aagatacggt gattgcagaa tttataggaa gttttagagg gaatgtgaca actcaaccta
    25321 gacgaagttg gagtataaaa cttttaaata atagtggaga gttgaaagca tttcattatg
    25381 aattcttaat aatagaaagt gaagatgtaa caatcacttt aagtagtggc tctgctggat
    25441 attttatgaa tgatggtgga agcaagccac agctcacagt ccgtgatcgt attgcaggtt
    25501 ttatgcattt ttctcactat gacttagaag aagctcgtaa aatacttgca gaatatggga
    25561 tcacattaat caattgggaa tatgcgccac cgataagaaa ttttttcaat gattattatt
    25621 agtcttataa aaaacagaat gtaaaattta tgtgcgcgag tatttagttt tccatttttt
    25681 gtttgataga tatatagcat tgttgaagca tgggatagtg gtgccaattt tatcaatgag
    25741 gttggtcagg cagttaacat aattggtgat gctataaatc catttaattg gaattggagg
    25801 aattaggtgt ggaaacagtg agattatgga tattcatgaa aaattttcgt tcggctttga
    25861 gatatgttat tctggaatca aaatcaggtt attatctgat tgacacagat cactatactt
    25921 tttggggagg ctaccttttt ccgattttta attggataat cccccataag gttatgaaaa
    25981 ttaacttgcc tgcagaagaa ataaatagat taatactcaa caaagattta caaaggaaaa
    26041 gagagaaaga acattctaat gttgcttgga taggtccagc atttgcagta gtgacttctt
    26101 caattgtcgg tcctatgcta gagagtattt atagtgattt ttctattgga atacgttatt
    26161 tatttgttac tttaagtata gctgttcttt tgtgcataaa gggttatgtt agcaagaaga
    26221 ataaagatat tttaacacta atcggtgtaa acaatttatc tagtactaaa attatactgt
    26281 ttcctgaatc tataaaagaa ggatcaaggt tgttgataat ttttataatg cttatactgt
    26341 tttctgtttt atttggggtg atgtttatag
//
LOCUS       DASJRQ010000036        25754 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJRQ010000036 DASJRQ010000000
VERSION     DASJRQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.036"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="U4A80_001308"
     CDS             complement(164..388)
                     /locus_tag="U4A80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN1974939.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="U4A80_001309"
     CDS             complement(586..2166)
                     /locus_tag="U4A80_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1974940.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="U4A80_001310"
     CDS             complement(2567..3943)
                     /locus_tag="U4A80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1974941.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="U4A80_001311"
     CDS             complement(4071..5189)
                     /locus_tag="U4A80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1974942.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="U4A80_001312"
     CDS             5331..5765
                     /locus_tag="U4A80_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974943.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="U4A80_001313"
     CDS             complement(5843..6376)
                     /locus_tag="U4A80_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1974944.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4A80_001314"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="U4A80_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1974945.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4A80_001315"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="U4A80_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1974946.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="U4A80_001316"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="U4A80_001316"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1974947.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="U4A80_001317"
     CDS             complement(10101..10679)
                     /locus_tag="U4A80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1974948.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="U4A80_001318"
     CDS             11001..11471
                     /locus_tag="U4A80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1974949.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="U4A80_001319"
     CDS             complement(11579..12805)
                     /locus_tag="U4A80_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1974950.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="U4A80_001320"
     CDS             complement(13559..13759)
                     /locus_tag="U4A80_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1974951.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="U4A80_001321"
     CDS             complement(13893..14501)
                     /locus_tag="U4A80_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1974952.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="U4A80_001322"
     CDS             complement(14586..15056)
                     /locus_tag="U4A80_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1974953.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="U4A80_001323"
     CDS             complement(15104..16018)
                     /locus_tag="U4A80_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1974954.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="U4A80_001324"
     CDS             16164..16967
                     /locus_tag="U4A80_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1974955.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="U4A80_001325"
     CDS             17145..18092
                     /locus_tag="U4A80_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1974956.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="U4A80_001326"
     CDS             complement(18160..18453)
                     /locus_tag="U4A80_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN1974957.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="U4A80_001327"
     CDS             complement(18481..19146)
                     /locus_tag="U4A80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1974958.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="U4A80_001328"
     CDS             complement(19286..19918)
                     /locus_tag="U4A80_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974959.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPVIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="U4A80_001329"
     CDS             complement(19925..20992)
                     /locus_tag="U4A80_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1974960.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="U4A80_001330"
     CDS             complement(20989..21720)
                     /locus_tag="U4A80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1974961.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U4A80_001331"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="U4A80_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1974962.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U4A80_001332"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="U4A80_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1974963.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="U4A80_001333"
     CDS             23883..25052
                     /locus_tag="U4A80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1974964.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..>25754)
                     /locus_tag="U4A80_001334"
     CDS             complement(25319..>25754)
                     /locus_tag="U4A80_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974965.1"
                     /translation="TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIY
                     SSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESG
                     INKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         7759 a         5162 c         4127 g         8706 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg acaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agtt
//
LOCUS       DASJRQ010000037        25560 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJRQ010000037 DASJRQ010000000
VERSION     DASJRQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.037"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..2222
                     /locus_tag="U4A80_001335"
     CDS             162..2222
                     /locus_tag="U4A80_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1974966.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2305..2874
                     /locus_tag="U4A80_001336"
     CDS             2305..2874
                     /locus_tag="U4A80_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1974967.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3049..3855)
                     /locus_tag="U4A80_001337"
     CDS             complement(3049..3855)
                     /locus_tag="U4A80_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974968.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3993..5507
                     /locus_tag="U4A80_001338"
     CDS             3993..5507
                     /locus_tag="U4A80_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1974969.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5910..6893)
                     /locus_tag="U4A80_001339"
     CDS             complement(5910..6893)
                     /locus_tag="U4A80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1974970.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6898..7545)
                     /locus_tag="U4A80_001340"
     CDS             complement(6898..7545)
                     /locus_tag="U4A80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1974971.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7630..8262)
                     /locus_tag="U4A80_001341"
     CDS             complement(7630..8262)
                     /locus_tag="U4A80_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1974972.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8278..8829)
                     /gene="coaC"
                     /locus_tag="U4A80_001342"
     CDS             complement(8278..8829)
                     /gene="coaC"
                     /locus_tag="U4A80_001342"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1974973.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8826..9563)
                     /locus_tag="U4A80_001343"
     CDS             complement(8826..9563)
                     /locus_tag="U4A80_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1974974.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9560..10549)
                     /locus_tag="U4A80_001344"
     CDS             complement(9560..10549)
                     /locus_tag="U4A80_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1974975.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10807..11349
                     /locus_tag="U4A80_001345"
     CDS             10807..11349
                     /locus_tag="U4A80_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1974976.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11366..12451
                     /locus_tag="U4A80_001346"
     CDS             11366..12451
                     /locus_tag="U4A80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1974977.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12463..13269
                     /locus_tag="U4A80_001347"
     CDS             12463..13269
                     /locus_tag="U4A80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974978.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13270..14097
                     /locus_tag="U4A80_001348"
     CDS             13270..14097
                     /locus_tag="U4A80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1974979.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14094..15167
                     /locus_tag="U4A80_001349"
     CDS             14094..15167
                     /locus_tag="U4A80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1974980.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15595..18291
                     /locus_tag="U4A80_001350"
     CDS             15595..18291
                     /locus_tag="U4A80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1974981.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18368..18856
                     /locus_tag="U4A80_001351"
     CDS             18368..18856
                     /locus_tag="U4A80_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1974982.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18868..19362
                     /locus_tag="U4A80_001352"
     CDS             18868..19362
                     /locus_tag="U4A80_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1974983.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19383..20192
                     /locus_tag="U4A80_001353"
     CDS             19383..20192
                     /locus_tag="U4A80_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1974984.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20189..21010
                     /locus_tag="U4A80_001354"
     CDS             20189..21010
                     /locus_tag="U4A80_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1974985.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21061..23160
                     /locus_tag="U4A80_001355"
     CDS             21061..23160
                     /locus_tag="U4A80_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1974986.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23157..24275
                     /locus_tag="U4A80_001356"
     CDS             23157..24275
                     /locus_tag="U4A80_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1974987.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24314..25192
                     /locus_tag="U4A80_001357"
     CDS             24314..25192
                     /locus_tag="U4A80_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1974988.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25338..25535
                     /locus_tag="U4A80_001358"
     CDS             25338..25535
                     /locus_tag="U4A80_001358"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1974989.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8075 a         4451 c         5411 g         7623 t
ORIGIN      
        1 aaagtacata agttattatt ccgattttct tgaaacaata aaaaatcaaa aaaaatactc
       61 tcctaatagt ccatcttctt ttgattttat ttatataatg aaatcatctt atgaaaatta
      121 ctacttcatg tttagacaag agtagggagg actaagaata aatgtgttca aaaagacgtt
      181 caaaacaact aaataaacac gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac
      241 actacgttaa gtttatgatg cgcacgatgc tgatcagttt atctgtaggt gtctatatgc
      301 atgtacccgt tcaggcaacg acacaaacca tcaaggtcgt acgtacctct gtttctgaga
      361 ccactgattc ccctatcact attactcata ctttcactga tgcaaatgga aaacagctac
      421 ttcctgcatt gacttcctat aataaagtca gttatcctcc aacgataaac cactactggt
      481 taattcctaa caaaacatta tggacatcgg aaaccggaag cagtgtgccg ctttctatct
      541 tttccgatat gactgatcct acaaaaggtg atcagttaaa acagatcact gatattttga
      601 atcatgcttt gacagatata aaggacggtg gaagcgcaca agtttcttat gtatatgaaa
      661 aagattattc acagtttgaa agaaagaaaa tcgatgttct ggcagggaca aaagtttcga
      721 tatctgatct ggtagaaagg ctgaagaacg cagatggaac acaaggagat atgtccaaag
      781 taatttcaaa caatgggaat atcgtggata cgactaatac aggaacatct actatttcct
      841 tgtcctattt tgatccaatt gctttaaaaa caatgactgc atctgctgtg atccatgttc
      901 gtgctgatca aacagctatc aaaggaaaag atcttcactt aagaacagga gagagttacg
      961 cccctaaaga tcttgttgaa tatgtaacaa aaagcgatgg aacttcagga aataaagaag
     1021 aaatcaaaat gaacgggaca gtagatacta caactccggg agaatatccc gttgtattaa
     1081 cttatataga ctcgaaaaca tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag
     1141 catctgctgc ttcatcgact gtcatcactc attttataga tagtcaaaca aataaagaac
     1201 ttcaagcaga aacacatatg agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg
     1261 aattttctga tcgagtgcca aatatagatt tatcgactta tgaacaaaat gggaaagtca
     1321 cttctatttc tgattggatg caagcaaacg gattagacag aaaaagtcac tggtcggaga
     1381 tccttgaagt acttcactca gaactgatga ataataggaa gcaatcagga ggggaaatta
     1441 cctttactta tgtttatata ccagacaatc gttcattaga aggtcatgat cttattgttc
     1501 atccaaacga aaaaataaac aaagaagagt tgatccaatc agcaacaaat tccttaggta
     1561 aacccgtacc gttaaaggat gtatcggtta caatcaatga agtaccattg gaggatggtt
     1621 atttgaccaa acaaacaggt gattttacga ttatgtatag ttatcatgac ccgtattcac
     1681 tccaagaact ccatgctcag tcactattac atgttttacc attaaacaaa cagatagatc
     1741 aaaaagaaca aaaaagacaa gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg
     1801 cagtttctcc aacagggaaa gtaaaacaaa caagaagcct agagttagca agaatatcta
     1861 gaaaaatctc agaatctaat aaaacgataa ggcaagaagc tcatttggcc ccacaagttt
     1921 ctggattgga aaatgaaatt ttagagccta aaataaaaca acaatcagaa aaagaatttg
     1981 ttgctaaaca gtcggaagat cagaaggaat cagatgacca gaaggaaacg aagaaaaaac
     2041 gcttgtcaac tattgattcc accgatgagc atctgcagcc gcaagtacca cttcctagtc
     2101 cgggaacacc cgcacctggt ggaggtattc cttcttctgc tttaggagat ttcatgcgag
     2161 gtatggcagg aacatgggta tacgctaatc gaaatgaatt tgacgcagat gactggttct
     2221 gatttatatc tctatctgtt atagtcaaat aagtagctga gtcatatgtt atttggcata
     2281 atggagacga ggagggatag aaaaatgtta gataaaacgt taccttacgc agaaatctgg
     2341 atgacaagac cacttcaaca aaatgttcca atcacgcctt tagcggaagg ttttgaattt
     2401 cgattttatc aaaaagaaga tgaatctgct tgggcggaga ttgagacttc cgttggggaa
     2461 tttcaaacga cagatgaagc acttgagtat tttcaacaaa actttgctcc ttatcctgaa
     2521 gaacttgaaa gaaggatgtt ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt
     2581 accgcttggc gaaaaaaatc aagagagcaa gtgtatccgg tttttcattg gttagcagtg
     2641 aaaccagatt accaaggaaa agggctcgca aaagcattga ctgccagagt cttgcaagaa
     2701 tttcctaccc tccatacgga cggaccaatc tatcttcaca cacagacatg gagccatcag
     2761 gcgattgctc tttataaaaa aatgggtttt acttttattg ctgaaaatct agacggcaca
     2821 cctaatccag attatgaaaa agttatgcag gttttaagta aattagataa ataacgaaat
     2881 ggtgaaataa gtcctcagta ttgggataaa aaacgaaata gtcattcctc atatcttact
     2941 tttttgagtt aatactatcg gataactaat ggcatgccat acattcaaaa aagaaggggc
     3001 agtgacaaaa gtattgtcac tgccccttct cttgtttttt agcatgtttt atcgtttgat
     3061 ttttttagga ataaaattct tgatgaacgt aaatttttgg taatcgtttc gactatagag
     3121 gtaaccaaat acactgaaaa caactgctcc aataaaatta acaaataaat ccttcatagt
     3181 atccatgatt cccacgtcta agtaaccatt ttggatgatt gttttatctg ttttaccatc
     3241 tgcattttga ctttcaatga ttgtccgagt gatatctgga acatgataga cagtgtttcc
     3301 gtctttactt aactccacac tattgatttg ctggatgatc gtatcttttt gcatatcgaa
     3361 ttgcagtaat ttatctgctg aaaattccac aaactcccac ataacaccaa ctgtcatcga
     3421 aaaacagaag gaaacaactg ctagtaataa aggagtcaag ttcatggttt ttgtattttt
     3481 attgagcaaa taaacaaggg aaaagccgac acccccagct aaaaatccgc tgaatgcatg
     3541 tagaaaagta tcccacattg aaaaatagcc ataaaaatca cttaactctc ctaaaactgt
     3601 tgaactaaaa ataaaagata acaaaatgat ttctagcata tttggaatct gtatctttaa
     3661 tatcttttcc agaaaataag ggatttcaaa taaaattaat gtcagtaata aaagcattgt
     3721 atttccccag tgttggttcc agacctgatc cactagcata ataacgacga tgatacgtaa
     3781 taaaatatag attccgtttg tgattctacc tgcagttttt gtactgttcg taaaaaaggt
     3841 tttcaaatga ttcataaaat aactccttca tgtatatcct taattatact aagaagcgtg
     3901 tgtttaccaa agctatatga cttaaagaaa aacagagttg atattgtgaa aaaaatcact
     3961 taatatagta taaggagaaa agggggatac aaatgaaaaa attagttgct ggtttattaa
     4021 cagccgttgc acttcttgct atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga
     4081 caagttctag tacgaaggaa tctgccgaag tatcatcagg cgcttctgaa caagtatata
     4141 cagatccaaa agaaatgaaa gaatcttatg atgtcgtgat tattggagct ggtggtgcag
     4201 ggatggcagc agctatcgaa gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga
     4261 tgcctgttgc cggaggaaac acatctaaat catcaagcgg tatgaatgct tcagaaacaa
     4321 aatttcaaaa agaagaagga atcaaagact cgaatgatgc cttctatgaa gaaacgctaa
     4381 aaggtggtgg aggaacgaat gataaagaaa tgcttcgata ctttgtcgat cactctgctg
     4441 atgcggtcga ttggctagat caaaacggta tcaaactgga caacttaacg atcactggag
     4501 ggatgagtga aaagaggaca catcgtccgt ctgatggctc ggctattggt ggatacttag
     4561 tggaggggtt attgaataac gtccatgagc gggaaatccc tatttttgtc aacgcagatg
     4621 tgacagacat taagaaaaat ggggaaacgg tcaatgaagt gactgtacat gttcaaggag
     4681 aagaaccaaa agaggtcacc ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca
     4741 gcaaagaatt tattgaaaaa tatcgtcccg atttagctga ttatattacg acaaatcaag
     4801 aaggatcaac tggagatggt atcaccatga ttgaaaaatt aggtggacaa accgttgata
     4861 tggataaaat acaaattcat ccaactgtgc agcaggatga gggggtcttg attggtgagg
     4921 ttgttcgcgg agaaggagca atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa
     4981 tggatacaag ggataaagta tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt
     5041 tgatttttga ccaaggcgtg cgtgatcgtg ccacagctat tgaattttat gaccaaaaag
     5101 gatatgtcac aactggaaag acaatggaag aattagcaga aaaaatcgat ttaccaaaag
     5161 aaacactagc aaaaacagtt gatggatgga atacggatgt tgcggagaaa aaagatgctc
     5221 aattcaaccg tgccacagcc atggaacatg acttatcgtc tccgaattat tatgcgatca
     5281 aaatcgcacc aggaatccat tacacgatgg gcggtgtgaa aatcaatacg aacactgaag
     5341 tattagataa agaaaaccag ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg
     5401 gcttacatgg aaacaaccgg atcggtggaa attcagttgc ggagatcatt atttttgggc
     5461 gacaagcggg catacaagca gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa
     5521 taaaaagagg ttgtgaaatc agcgttttga gctaataccg agggtcagct gattgaacac
     5581 tgtttagtga agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
     5641 aaaacagctt cttatgtttt taacttggtt ggacttatta ccgcagatgc aagcttttga
     5701 acaccgttta ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
     5761 ctaaacacgt ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac
     5821 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
     5881 tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg
     5941 atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta
     6001 cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa
     6061 gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt
     6121 tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc
     6181 ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa
     6241 gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc
     6301 tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa
     6361 aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta
     6421 gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg
     6481 ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag
     6541 attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc
     6601 atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga
     6661 gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc gactcccaaa
     6721 atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca
     6781 gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca
     6841 atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta
     6901 ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac
     6961 tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt
     7021 agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac
     7081 ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt
     7141 cagccattca tctgcataat gtttagtaat ataataatct gtcatggact caggccattt
     7201 tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc
     7261 agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg
     7321 agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac
     7381 ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga
     7441 agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag
     7501 gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc
     7561 gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta
     7621 cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac
     7681 tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc
     7741 tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg
     7801 gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc
     7861 agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata
     7921 aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt
     7981 ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg
     8041 tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc
     8101 acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc
     8161 attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc
     8221 taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta
     8281 agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa
     8341 ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata
     8401 gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt
     8461 gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga
     8521 aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc
     8581 gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga
     8641 ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt
     8701 gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa
     8761 ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat
     8821 ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat
     8881 ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg
     8941 ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta
     9001 ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg
     9061 atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa
     9121 acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca
     9181 ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc
     9241 agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata
     9301 atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca
     9361 gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta
     9421 gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct
     9481 aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct
     9541 ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt
     9601 gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga
     9661 cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat
     9721 ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca
     9781 agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat
     9841 aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt
     9901 cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga
     9961 cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggtaa
    10021 tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag
    10081 aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca
    10141 cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat
    10201 tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg
    10261 gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg
    10321 gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc
    10381 gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg
    10441 caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg
    10501 tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac
    10561 tccttttaaa attttctttt atcataccat aaagcctact cttttattct tgttcttccg
    10621 taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa
    10681 agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc aataaatttc
    10741 gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt cgattcggag
    10801 gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa tttgacacaa
    10861 gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt ggaacgagat
    10921 ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg tgtgactcca
    10981 gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa agaagatcat
    11041 acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc tgattcgaat
    11101 gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata taagacgttt
    11161 gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga gttaagctta
    11221 ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc gacaaaacaa
    11281 catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc aacgcaatca
    11341 tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg atcaagtagt
    11401 gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa
    11461 attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac gtattatagc
    11521 tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt tgaatgattt
    11581 gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt ttcctcatat
    11641 gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca aatcagatat
    11701 cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg aaaaacggga
    11761 gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa
    11821 tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga aactacgtac
    11881 agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt ttatttttgt
    11941 tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca tgaataaagg
    12001 gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca atcatttcgt
    12061 tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag ataatctagt
    12121 tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt
    12181 tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag gaaaactagt
    12241 cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct gttatgatga
    12301 acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg
    12361 attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg aagaagaatt
    12421 cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac aatgcgcagg
    12481 atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg
    12541 atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa ttatctatct
    12601 tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta tgcatttttg
    12661 atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac aaaactgaag
    12721 cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct gctgcttaaa
    12781 gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt ctttgatttt
    12841 atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt cgtggcaacg
    12901 tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga gatgaatcct
    12961 tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt ccgacgagtg
    13021 atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt tattccttct
    13081 ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg
    13141 gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac aatcggtgtg
    13201 attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt
    13261 cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt ctttttattc
    13321 ttatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg aacgatgaac
    13381 agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac acgtttgatc
    13441 attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc cacgatcatc
    13501 gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg gacagcgttg
    13561 ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc tagtttcttg
    13621 attttcttta cactactagg attcggatta ggttttcaga gtgtattgct ttcccatatt
    13681 gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga aatgaacgat
    13741 tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt caaaaatatc
    13801 attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt tacctattct
    13861 cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac gctctcagta
    13921 gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta
    13981 gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa agaaaaccgt
    14041 ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct gcgatgaaaa
    14101 aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg cttggtgttc
    14161 gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc tataactggg
    14221 gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga tacaaagtga
    14281 attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa
    14341 actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa gaaaaactag
    14401 tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca cgatttttag
    14461 acttggattt tgatccagat aatcagtatt cgattccata tttttgggga actttaggga
    14521 tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat gatttgtgga
    14581 agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt atggggcttt
    14641 ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagctg acagaagcag
    14701 caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa
    14761 tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa gctgccgata
    14821 tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc aatctttggt
    14881 ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat gatttcatca
    14941 actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt tattctacac
    15001 caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa caattctatc
    15061 catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg aaatacctag
    15121 gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg caaaactcag
    15181 taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt ctatctgagt
    15241 tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat tttatttttt
    15301 ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac
    15361 gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg aaactatgca
    15421 aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc agttcaatat
    15481 ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg tagataaatt
    15541 attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta aaaattggaa
    15601 tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa
    15661 ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta
    15721 acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt
    15781 cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg
    15841 ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa
    15901 ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa
    15961 aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc
    16021 acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa
    16081 attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc
    16141 gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag
    16201 gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat
    16261 cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt
    16321 ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg
    16381 gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg
    16441 ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt
    16501 caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta
    16561 atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt
    16621 tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc
    16681 ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg
    16741 ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt
    16801 ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata
    16861 aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt
    16921 agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac
    16981 ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca
    17041 agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg
    17101 acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac
    17161 tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg
    17221 aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat
    17281 caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca
    17341 gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg
    17401 ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa
    17461 ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag
    17521 atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc
    17581 aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg
    17641 ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc
    17701 atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa
    17761 acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca
    17821 attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat
    17881 attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa
    17941 ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg
    18001 agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc
    18061 cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct
    18121 cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat
    18181 tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct
    18241 tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa
    18301 aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat
    18361 taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa
    18421 aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt
    18481 tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta
    18541 tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca
    18601 ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa
    18661 aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct
    18721 gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa
    18781 taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga
    18841 ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta
    18901 attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta
    18961 gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca
    19021 tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg
    19081 cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt
    19141 gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca
    19201 ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt
    19261 caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg
    19321 atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa
    19381 ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggagttgct ggacatgaaa
    19441 tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga
    19501 tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg
    19561 tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag
    19621 cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag
    19681 cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg
    19741 ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct
    19801 ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg
    19861 gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa
    19921 cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga
    19981 gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac
    20041 cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc
    20101 tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc
    20161 ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga
    20221 gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc
    20281 ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta
    20341 caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac
    20401 cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga
    20461 aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt
    20521 ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc
    20581 gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa
    20641 tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa
    20701 atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat
    20761 cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt
    20821 gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt
    20881 taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt
    20941 cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg
    21001 aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag
    21061 atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac
    21121 gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag
    21181 ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg
    21241 attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg
    21301 gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt
    21361 gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg
    21421 cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata
    21481 ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac
    21541 gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag
    21601 caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca
    21661 agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa
    21721 tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag
    21781 ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt
    21841 tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct
    21901 gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt
    21961 gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct
    22021 ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta
    22081 ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag
    22141 gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa
    22201 ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc
    22261 ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta
    22321 gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt
    22381 tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat
    22441 gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta
    22501 atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa
    22561 gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat
    22621 cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa
    22681 ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg
    22741 agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca
    22801 gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc
    22861 gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg
    22921 atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat
    22981 aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt
    23041 acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg
    23101 aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga
    23161 gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa
    23221 atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca
    23281 aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg
    23341 gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt
    23401 ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg
    23461 aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt
    23521 tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa
    23581 catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata
    23641 cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt
    23701 ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg
    23761 gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg
    23821 gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa
    23881 caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac
    23941 gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag
    24001 atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg
    24061 acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg
    24121 aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc
    24181 aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac
    24241 gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag
    24301 ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc
    24361 agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt
    24421 tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat
    24481 cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat
    24541 cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata
    24601 tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt
    24661 tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg
    24721 aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca
    24781 tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga
    24841 ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca
    24901 gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc
    24961 atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa
    25021 acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt
    25081 tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat
    25141 aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa
    25201 tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt
    25261 tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat
    25321 taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata
    25381 ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt
    25441 ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca
    25501 taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata
//
LOCUS       DASJRQ010000038        24308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJRQ010000038 DASJRQ010000000
VERSION     DASJRQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.038"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(112..657)
                     /locus_tag="U4A80_001359"
     CDS             complement(112..657)
                     /locus_tag="U4A80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1974990.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(669..1361)
                     /locus_tag="U4A80_001360"
     CDS             complement(669..1361)
                     /locus_tag="U4A80_001360"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1974991.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1380..1664)
                     /locus_tag="U4A80_001361"
     CDS             complement(1380..1664)
                     /locus_tag="U4A80_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1974992.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1677..2240)
                     /locus_tag="U4A80_001362"
     CDS             complement(1677..2240)
                     /locus_tag="U4A80_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1974993.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2468..3130
                     /locus_tag="U4A80_001363"
     CDS             2468..3130
                     /locus_tag="U4A80_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1974994.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3254..4450
                     /locus_tag="U4A80_001364"
     CDS             3254..4450
                     /locus_tag="U4A80_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1974995.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4551..5927)
                     /locus_tag="U4A80_001365"
     CDS             complement(4551..5927)
                     /locus_tag="U4A80_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1974996.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5964..6830)
                     /locus_tag="U4A80_001366"
     CDS             complement(5964..6830)
                     /locus_tag="U4A80_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1974997.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6919..7944)
                     /locus_tag="U4A80_001367"
     CDS             complement(6919..7944)
                     /locus_tag="U4A80_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1974998.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7962..8636)
                     /locus_tag="U4A80_001368"
     CDS             complement(7962..8636)
                     /locus_tag="U4A80_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1974999.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8820..9713)
                     /locus_tag="U4A80_001369"
     CDS             complement(8820..9713)
                     /locus_tag="U4A80_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1975000.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9787..11484)
                     /locus_tag="U4A80_001370"
     CDS             complement(9787..11484)
                     /locus_tag="U4A80_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1975001.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11477..12790)
                     /locus_tag="U4A80_001371"
     CDS             complement(11477..12790)
                     /locus_tag="U4A80_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN1975002.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12790..16827)
                     /locus_tag="U4A80_001372"
     CDS             complement(12790..16827)
                     /locus_tag="U4A80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1975003.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16840..17298)
                     /locus_tag="U4A80_001373"
     CDS             complement(16840..17298)
                     /locus_tag="U4A80_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1975004.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17596..18114)
                     /locus_tag="U4A80_001374"
     CDS             complement(17596..18114)
                     /locus_tag="U4A80_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1975005.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18116..18732)
                     /locus_tag="U4A80_001375"
                     /pseudo
     CDS             complement(18116..18732)
                     /locus_tag="U4A80_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(18729..19592)
                     /locus_tag="U4A80_001376"
     CDS             complement(18729..19592)
                     /locus_tag="U4A80_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1975006.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19579..20490)
                     /locus_tag="U4A80_001377"
     CDS             complement(19579..20490)
                     /locus_tag="U4A80_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1975007.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20531..21865)
                     /locus_tag="U4A80_001378"
     CDS             complement(20531..21865)
                     /locus_tag="U4A80_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1975008.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22073..22903
                     /locus_tag="U4A80_001379"
     CDS             22073..22903
                     /locus_tag="U4A80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN1975009.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23147..23465)
                     /locus_tag="U4A80_001380"
                     /pseudo
     CDS             complement(23147..23465)
                     /locus_tag="U4A80_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23587..23781)
                     /locus_tag="U4A80_001381"
     CDS             complement(23587..23781)
                     /locus_tag="U4A80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975010.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT         7142 a         5281 c         4208 g         7677 t
ORIGIN      
        1 aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt ttgttttatg
       61 ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt tttataaaat
      121 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
      181 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
      241 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
      301 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
      361 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
      421 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
      481 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
      541 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
      601 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
      661 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
      721 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
      781 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
      841 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
      901 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
      961 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
     1021 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
     1081 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
     1141 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
     1201 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
     1261 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
     1321 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
     1381 tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat aataagagat
     1441 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
     1501 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
     1561 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
     1621 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
     1681 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
     1741 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
     1801 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
     1861 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
     1921 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
     1981 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
     2041 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
     2101 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
     2161 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
     2221 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
     2281 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
     2341 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
     2401 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
     2461 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
     2521 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
     2581 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
     2641 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
     2701 ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca
     2761 gcagacagcc aaacttaaag caatcgatct ccgccatgat ccagtcaaag cagcgaaagc
     2821 tttattcaaa gaattggtga aagaaccaaa ccgactgcat gaagcaagtc agtttcttta
     2881 tacgcatctg ccgaatctag ttgatttgac aaataagtat aatgagatca atgaccatga
     2941 gatcaaaaac aaacagacct atgaaaaact agaagaaagc gcgcagatca tcgaccagct
     3001 gtcagcttta atcgcacaag attatcaaaa attcgttgct gaggatctag atgatatcga
     3061 cgtagagata tctgttgcga aacagagctt aaagcgtgat aataaatatg aaccagaaca
     3121 aaaggattaa ctcttttgtt cataaaagaa gactcggaac attcagatga tgtctggctg
     3181 tttcgagtac tttttctaaa gtagagacat tcatgtaaga atcgagaaca gattcaacaa
     3241 ggaggaattt tttatggatc ataaaccgac tgaaacaaaa gatgttacac cagttaacga
     3301 tacacttgaa gatttattga acaacccgtt ttcaactcca acagatacgc tgaccacttc
     3361 acaacaagcg gagattgatc agctccaaga gcaacagaca gctgctcgtt taattgacaa
     3421 actaccggcg gaacgccaag aacaagctaa acagctagca gcaaaaatcg atgctaatga
     3481 tgcacagtct gtcatcagct acggatcggc tgcccaagca aaattaagtg aattctcaca
     3541 atcgatgttg aatcatgtgc aagcacaaga tattggtcct gtaggagatt ccttaactga
     3601 actgatgtat cgtctccaag aagccaatcc tgacgaactg cgtgccggag aagggaacat
     3661 cttccagcgt gtcttcggga aggtcaaaca atcgatctat gaagtgacag caaaatatca
     3721 aaagattggt gcacaaattg acaagatttc agtgaaatta gataaagaaa aagacggtct
     3781 gctaaaagat aatctgatgt tagaacagct ttaccagaaa aacaaagact actttgatgc
     3841 actgaatatt tatatcgctg ctggagaatt gaaaatggaa gaacttcaaa caacgatcat
     3901 tccagaagcg atgaaaagag cagaagaaac tggcgatcag atggatgttc agattgccaa
     3961 tgattacacg caattcctag atcgtctgga taaacgtacg catgatcttc gcttagctag
     4021 acagatcacg atccagcaag caccacaaat ccgtttgatc caaaacacaa atcaggcctt
     4081 ggctgaaaag atccaagcat ctatcgctac agccattccg ctgtggaaaa accaagtcgt
     4141 gatcgcattg accttgcttc gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga
     4201 aacaacaaac gacttattga agaaaaattc agaaatgctg aaaatctcag ccatcgaaac
     4261 agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc cttcaaacca ctcaaaatga
     4321 tttgatcgaa acaatccaag agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg
     4381 tcatgcagaa gtagagcttg gacacatgga agaagatctg aaacaaaaac tgttggattt
     4441 aacccaatag atcacctttc gcttgttact aaaacaaacg cccaacaaca gtttattaca
     4501 actgttgatg ggcgtttttc ttatctttct tctaacttct ccattggagt ctatttctca
     4561 ttcatcttgc tgtgtccata gccatagcca aaataaatga tcagtccaat aaccattacg
     4621 ataccgaaca cgatccatgt cacagtttgc agacgaagca tcaatacaat acaagtcaag
     4681 atggagacga ttggcaatag cggaacgaat ggcactttga attctccagc ttttggtttg
     4741 ccaaacattt ttctcaactt cagtaagcct agactaacaa gcatcaaata ggcgagcgcc
     4801 atgatattcg ttagttccgc taatacattc aatgggaaaa atccggcaaa gaatacagct
     4861 gccacaccag cggcataagt cgcttttttc ggtgtacgat gcttttgatc gatttctgtc
     4921 ataaacttag gaagtaagcc atccttgctg acagcaaaca gtaaacgagc aagtgaatac
     4981 atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc ctacagcgat caccgcgccc
     5041 actgttccat gtcctacata cctcattgca aaagccactg ggtcattgac tcctagttca
     5101 gtatacggaa cgattcctgt caagatcagc gtcacgatga tgtaaaggac agtagcaatc
     5161 agtatcgaac caatgattcc tcttggcaca tctttttgcg ggtttttgac ttcctcggct
     5221 gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca caagagctgc accatctagc
     5281 acaccggaaa aaccaaaagg catgaatggc tgccagttat caggtttaac gaaaaatatt
     5341 cctacaatga taaacaacgc gatgatacca aatttgacat ataccatggc attattcaaa
     5401 cgtaatgctt tcttggcttc aagcgacacg atgtaagtga cgaatatcac tacgagcacc
     5461 gcaatcaagt caatgtatgt accattttcc ggattgtacc cgccagttaa tgcttgtgga
     5521 agttcggccc ctaaactttt caaaaaacct tgcatatagc cagaccatcc ggaagcaaca
     5581 gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat
     5641 tcaccaaata caacatacag ataggcatat ggcccaccaa tcactggcac tcgcgaagca
     5701 aattctgcga aacttagtcc agacaaaatg acaacgattg cagctaagac aaatgaaagt
     5761 gacagtgctg gaccagcatt ttgattggcc gcaacccctg tcaccacaaa tattcccgta
     5821 ccgatgatcg caccaattcc aagcatgatc aaatcagacg tttttagttc ttttttcatc
     5881 tcactcgtat gattgagatt gatctctttc ttacgaaaca aatccattga agttcctccc
     5941 cattttaggt tggaaaacag cgattaaact gttttcatat accctaaaag gataccacct
     6001 aaaatgacta aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg
     6061 ccaaggatga aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag
     6121 ccaacagcta aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat
     6181 aataatccac agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca
     6241 aagaagcttg cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc
     6301 aaattcgccc agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat
     6361 gctctaaaac ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca
     6421 tcttgtcggg cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat
     6481 acataatctt tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa
     6541 attaattgaa aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca
     6601 tggaactgtc cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa
     6661 atccaagggg tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca
     6721 ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa
     6781 ccaatactac cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc
     6841 cttttatttc acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt
     6901 ttcgtttcta acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa
     6961 agtcattgtt gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc
     7021 ggccagattt tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc
     7081 atttctccca gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat
     7141 ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat
     7201 gtttccgtga tccggcgtat atttgatggc attactaagc aattggttga agataaaagc
     7261 gatccattta gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt
     7321 tttttggata aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc
     7381 atattcttgg atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg
     7441 ttctacgtat tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc
     7501 aatatcgaac tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc
     7561 atggacccat gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat
     7621 tacttcttgg tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc
     7681 tgtcctccca ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt
     7741 tccccaccat cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc
     7801 ctcaatcaaa gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca
     7861 catcacaaag atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc
     7921 tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc
     7981 tgtcctactt ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac
     8041 cgattgatgt tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct
     8101 cgcagcaatt tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga
     8161 cgatattcat ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca
     8221 acattcaacg taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa
     8281 gaacggcgaa gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc
     8341 acaaaatcat ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg
     8401 gagataaaaa tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca
     8461 tccaaaacgg gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct
     8521 gagaacactt gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc
     8581 tccatgatca attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa
     8641 aatccctcca tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg
     8701 gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa
     8761 tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat
     8821 tatctgttat ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg
     8881 aaaagcgtta gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat
     8941 ttttttgtct gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc
     9001 actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc
     9061 tgcttttctc taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg
     9121 acttcataag cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt
     9181 tcttcaagat ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa
     9241 tgtgtactct ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct
     9301 aacaaaagat cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag
     9361 atggctccga tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc
     9421 gcttgctcaa aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc
     9481 gtctgatttt tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt
     9541 tcaagctgct tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct
     9601 gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat
     9661 tgtaataaaa gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc
     9721 accttcttat ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt
     9781 tgttgcttat acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat
     9841 caaacttatt ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga
     9901 cccgcgtttc atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat
     9961 ttcttcatta tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg
    10021 aagtacataa ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc
    10081 ttgaacctca aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc
    10141 ccactcaatt ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa
    10201 tattgcgtat tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc
    10261 actgcttgat gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg
    10321 ctctactcct tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt
    10381 gttatggaaa tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa
    10441 atctcctcca cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc
    10501 ttctgtcaaa atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc
    10561 ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc
    10621 aactgtaggc gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac
    10681 agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc
    10741 tagatcaata taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct
    10801 aaggtcgctt tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata
    10861 caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac
    10921 catatcaggc atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg
    10981 agctggcgaa taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc
    11041 cgtcacttgg acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat
    11101 acttgtcagt cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat
    11161 cgcttcggca gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa
    11221 atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc
    11281 accaaaatcc gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc
    11341 ttttttgtgt aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac
    11401 tagttttccg ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc
    11461 cgtgacaatc atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag
    11521 cacctaacaa taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg
    11581 ggaatacgga ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt
    11641 tgtccgttgt ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag
    11701 agactggaac tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt
    11761 gtggttggcc ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg
    11821 cttcatccat ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag
    11881 gcaagaaacg ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat
    11941 ttttgtcatt caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag
    12001 caggagccat atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag
    12061 caccttctaa tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt
    12121 atggatctct tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg
    12181 ttgattctgt cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga
    12241 tatcaacatc caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat
    12301 cccgatcttg gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa
    12361 ccgtatcttt cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta
    12421 gctgacggat attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca
    12481 tgttttcagc agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg
    12541 ctccatttag tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga
    12601 tagccaagcc atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca
    12661 tccgtttatg caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat
    12721 gtggaagaag gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca
    12781 gctttttcat taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc
    12841 tcctgcaagc gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc
    12901 tgtagcaggc aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt
    12961 tgtctccaaa agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt
    13021 tggataatac gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc
    13081 attttctatg tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt
    13141 tctatagctg tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga
    13201 agcattgaat agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata
    13261 ctctcctgca ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt
    13321 catactgtca attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac
    13381 ttgtagtaca ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat
    13441 gaccgtatct tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc
    13501 agatagccga tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag
    13561 atgcagaggt tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata
    13621 gaagtatccg atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta
    13681 tgctcgatac agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc
    13741 taatgtatgg ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt
    13801 gtaagcagtg aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg
    13861 ccaattttct tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg
    13921 caagatccat gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat
    13981 gattttgtat tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg
    14041 ctctccgatt ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc
    14101 tactgaatag aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc
    14161 tttcgctact ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc
    14221 tgcgccattg attggcatat tactgccttc aattagttta ttgatattca tgtggattgg
    14281 cttgaattca tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat
    14341 gttaggctct tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc
    14401 tttaccttcc ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg
    14461 acctaacatc tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc
    14521 tactaactga taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc
    14581 tccagtttca tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt
    14641 agttttactt gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc
    14701 aaattcgact ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg
    14761 gcctttatgt tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc
    14821 attctcataa tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc
    14881 gtcttgagct cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc
    14941 taaagaaatc ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt
    15001 cgccactact tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg
    15061 gtacttcatg ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc
    15121 agctccaaat ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt
    15181 tctcttgaag gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc
    15241 attgtaatat cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt
    15301 tcgtccaata gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat
    15361 ggagaagata cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac
    15421 tgtgttgatc gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga
    15481 agaaccatca ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg
    15541 atagtgtaca tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg
    15601 agtaaaccct gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc
    15661 attatcttca gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct
    15721 cgtacgataa cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac
    15781 gattaggatt ttttctggat ttctattttc tccgccattg tctttataca atgtttcata
    15841 gccattttta agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt
    15901 attctcactt cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat
    15961 ttgctcatgg ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat
    16021 atccagactc gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc
    16081 agcattttta gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc
    16141 tagaacaaaa acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag
    16201 atagacattg taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg
    16261 cagaacaggt tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc
    16321 aaaattccgt tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc
    16381 gcctgcagtt gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt
    16441 tgtataatca ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg
    16501 atagcgttca ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa
    16561 cttcgggtag cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg
    16621 gtcttgcgta gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat
    16681 gcgctggagt gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc
    16741 ttcgctacgt tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg
    16801 gttatctatt ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg
    16861 gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc
    16921 gacataccat tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata
    16981 ggaggataga cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc
    17041 gattgctaaa cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca
    17101 ttcaagggtt tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa
    17161 gaatacctca tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg
    17221 atagactgac tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt
    17281 caattttaac cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac
    17341 aatcaacaaa acaatgccca tctaattata atttaattta attttaattt taaatcaaat
    17401 gatatccttt ttagttaaaa aacaccaatt atacaacatt tattattaaa attaaattat
    17461 aaaattttat aacgttctca tttaacaaaa cttaagttat taagacaaat gaataaacaa
    17521 ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg catttacgca ctcatgcttt
    17581 attcgtacaa ttaattcaga taacggcagc gcataggatg ccaataacac ttcctaaaat
    17641 agctcctcct aaaatgtcag taggataatg taaaagtaaa aaaatcctcg aataagcgat
    17701 caaacaagca aataaaaagg ctaatattcc tgcaactgga cttaattcga acaacgcagc
    17761 tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact
    17821 tgtgtaaagg acaagccgac gatttttttc ataaggtctt ttccgtcttg tcgttttttt
    17881 gataaaaagc aaaccaacaa aaaaatagat caaactgccg ataagaatgg ctagaccata
    17941 ttctctagta tcaggtatca agactaaaac agcagcaagg agcaaccaaa taaaaccacc
    18001 attagagata ttactgataa atttcaaaaa attgattttt cgactatccg ttttccgttg
    18061 cttggcgaac ttgttgataa tactatcatc gattttagca atcaaacgca acatgttatt
    18121 cctccttttt aagaaccttt tcttgcgtac gatggataaa aagcaggact tgaaaataaa
    18181 acaagaaact gaaacctaat aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa
    18241 gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga
    18301 aaaatgcagt aatccaagaa gcttcttttt gttccagttg aagcaagatt ttgtaactaa
    18361 agagaagcgc gcttaataga agcaaaaaca acgtagtata catcaatgta tagccaattc
    18421 ccccacccca atagcggaaa atctgataat cgatcaatag aagaagaaaa taaacacttg
    18481 ctaaactatc ttgctttcca taaagagaga agttctcacg aaactttttc cagaatagtg
    18541 aaccgagctt tttctcttca tttttttgta atgataaatc atatacagaa ctgttcgact
    18601 tgcagttcct aatgtgaaaa caggcaagac gcacaggaac catagtagct gtaaatggat
    18661 aagacgtgga atccattcaa tgatttctaa gaaataatca ttggcttttt taccaatcga
    18721 tcgattcatc atccttttga acctcctgtc aaatccatcc cttcaatgaa ataacgttgt
    18781 gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg ttgctcctgc catcagataa
    18841 ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt
    18901 tcttgatctt ggatatataa cagaggacct aagaaatcat tccaagctgc gttgaaggaa
    18961 ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa gcatcaaatg actccaaata
    19021 tacaaatgat tcgctccgtc gatttcagca gcttcgatca attcattatt gatcgataag
    19081 taaaattgac gcatcagaaa tatattgaac gcattgccga agaacgatgg aacgatcagg
    19141 ggcaaataag tacctaccca gccgatcttg gagaataata catattgagg gatcatcgtt
    19201 acaaaccccg ggatcatcat cgtagaaaga accaaagcaa acatcagctt tttcccagga
    19261 aaatcaagct tcgcaaatcc ataagcaata aaagaatttg aaaggacatt accgatcact
    19321 actagcacag tgatgaataa tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc
    19381 gtatccagat agttttccca tacgattttt tctgggaaaa aggtcggagg ataacgaacg
    19441 atttcttcca ttggtttcaa tgaagtaaac accatccaaa gcaatggaat gatcaaaatg
    19501 attcctatta ctgtcatgag gacataactt agtatttttt tgtttgccga ttcacactgg
    19561 tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc acttaggtgc
    19621 aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca ttgacatagc
    19681 catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca gattataaaa
    19741 aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag cttcttgaaa
    19801 aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag gagtgattaa
    19861 tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt ctgctgattc
    19921 atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg ctcctccaga
    19981 actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa acagccaagc
    20041 tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag atggagaaag
    20101 cagctgcatc cataagatgt agactgctac tcctgataag actgccggta aatagaaagc
    20161 tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta aaaacacact
    20221 gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt ataacgattt
    20281 ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa tgaaattcat
    20341 cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca taggccctag
    20401 cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc atcttgcttc
    20461 ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta cctagacagt
    20521 caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt gcatctgcca
    20581 atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca ttcactaatg
    20641 tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta aagttttcgt
    20701 ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc actaaattct
    20761 cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt tcttgtactt
    20821 ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt gctccgtatg
    20881 gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct tctttttctg
    20941 ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa ctagagtaat
    21001 aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca gatacaccag
    21061 acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct tgccagtctt
    21121 ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt tcatctttgt
    21181 caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct aaattccata
    21241 atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt ttacgtgctg
    21301 tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca gcttctttaa
    21361 atatcttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc gcataaggat
    21421 caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa gtgtcttttt
    21481 ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca acggaattta
    21541 tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa gatttagtcc
    21601 agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt gattgattga
    21661 aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa aaggaccaga
    21721 aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca cttgaactgg
    21781 catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa cttgccagca
    21841 accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa ccgatgccgg
    21901 ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta tgattctaaa
    21961 aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt gaatttttta
    22021 atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt tgatgtttag
    22081 acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat taatcgcgaa
    22141 atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta tgcagtacct
    22201 gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga tggacattgc
    22261 aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt tattagaggg
    22321 tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg gtgccattat
    22381 ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc ctgctgacag
    22441 cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt tagcagactt
    22501 tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta tttacgaaat
    22561 gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag atagctatca
    22621 tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt caatcttagt
    22681 cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact tctataactt
    22741 ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac tggatggcga
    22801 aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa aattagaacc
    22861 agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta cccgtagaaa
    22921 cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat taatcataac
    22981 cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg ccagcgccta
    23041 aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt tttgcccacc
    23101 tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac tctcgtacac
    23161 tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt ttgatggtcg
    23221 cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt cgatacccaa
    23281 atttatattt tagatttgca caatctcttg atgatctcct gaatattggc tcgatattga
    23341 ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt acacatctac
    23401 tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt ggttttgtat
    23461 ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa cccgctgctt
    23521 cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct cttacggcaa
    23581 tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc caagaaataa
    23641 agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa cagaaatccc
    23701 gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct ttttatagaa
    23761 tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta atcacaaatt
    23821 cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct tcgactttcc
    23881 ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac ctctaaatct
    23941 aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca tccagtatac
    24001 ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc cattgcagga
    24061 gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat aggacttact
    24121 cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc aactacatgc
    24181 acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga atcgggaatt
    24241 gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata tctttattat
    24301 aaaataac
//
LOCUS       DASJRQ010000039        24227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJRQ010000039 DASJRQ010000000
VERSION     DASJRQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.039"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..1372
                     /locus_tag="U4A80_001382"
     CDS             71..1372
                     /locus_tag="U4A80_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1975011.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            1375..2226
                     /locus_tag="U4A80_001383"
     CDS             1375..2226
                     /locus_tag="U4A80_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1975012.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            2239..3054
                     /locus_tag="U4A80_001384"
     CDS             2239..3054
                     /locus_tag="U4A80_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1975013.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            3205..4371
                     /locus_tag="U4A80_001385"
     CDS             3205..4371
                     /locus_tag="U4A80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1975014.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            4601..5248
                     /locus_tag="U4A80_001386"
     CDS             4601..5248
                     /locus_tag="U4A80_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1975015.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            5332..6276
                     /locus_tag="U4A80_001387"
     CDS             5332..6276
                     /locus_tag="U4A80_001387"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1975016.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            6411..7862
                     /locus_tag="U4A80_001388"
     CDS             6411..7862
                     /locus_tag="U4A80_001388"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1975017.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            7883..8719
                     /locus_tag="U4A80_001389"
     CDS             7883..8719
                     /locus_tag="U4A80_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1975018.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            8719..9378
                     /locus_tag="U4A80_001390"
     CDS             8719..9378
                     /locus_tag="U4A80_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1975019.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            9504..10193
                     /locus_tag="U4A80_001391"
     CDS             9504..10193
                     /locus_tag="U4A80_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975020.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            10199..11155
                     /locus_tag="U4A80_001392"
     CDS             10199..11155
                     /locus_tag="U4A80_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1975021.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            11199..11711
                     /locus_tag="U4A80_001393"
     CDS             11199..11711
                     /locus_tag="U4A80_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975022.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(11967..12515)
                     /locus_tag="U4A80_001394"
     CDS             complement(11967..12515)
                     /locus_tag="U4A80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1975023.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(12604..13785)
                     /locus_tag="U4A80_001395"
     CDS             complement(12604..13785)
                     /locus_tag="U4A80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1975024.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(14021..15529)
                     /locus_tag="U4A80_001396"
     CDS             complement(14021..15529)
                     /locus_tag="U4A80_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1975025.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            15688..15879
                     /locus_tag="U4A80_001397"
     CDS             15688..15879
                     /locus_tag="U4A80_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975026.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            16082..16999
                     /locus_tag="U4A80_001398"
     CDS             16082..16999
                     /locus_tag="U4A80_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1975027.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            16992..18065
                     /locus_tag="U4A80_001399"
     CDS             16992..18065
                     /locus_tag="U4A80_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975028.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            18213..18731
                     /locus_tag="U4A80_001400"
     CDS             18213..18731
                     /locus_tag="U4A80_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975029.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            18712..19104
                     /locus_tag="U4A80_001401"
     CDS             18712..19104
                     /locus_tag="U4A80_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1975030.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            19180..20115
                     /locus_tag="U4A80_001402"
     CDS             19180..20115
                     /locus_tag="U4A80_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1975031.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            20248..21027
                     /locus_tag="U4A80_001403"
     CDS             20248..21027
                     /locus_tag="U4A80_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1975032.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            21020..21337
                     /locus_tag="U4A80_001404"
     CDS             21020..21337
                     /locus_tag="U4A80_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1975033.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            21581..23191
                     /locus_tag="U4A80_001405"
     CDS             21581..23191
                     /locus_tag="U4A80_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1975034.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            23348..24196
                     /locus_tag="U4A80_001406"
     CDS             23348..24196
                     /locus_tag="U4A80_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1975035.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         7731 a         4333 c         4820 g         7343 t
ORIGIN      
        1 attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg
       61 gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg
      121 ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt
      181 tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt
      241 aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga
      301 aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt
      361 agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa
      421 ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta
      481 tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac
      541 taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa
      601 cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg
      661 gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc
      721 ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt
      781 accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag
      841 cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat
      901 cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca
      961 gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc
     1021 agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa
     1081 catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg
     1141 gaatttcaat aatttctcca tgatctatct gtttaacaat ggtggtcccg gaagcgtagg
     1201 cggaggagct ggttctactg atattcttat ttcctggatc tataaactta cgactggaac
     1261 ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg
     1321 tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa
     1381 aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt
     1441 cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc
     1501 aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac
     1561 tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt
     1621 tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt
     1681 cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta
     1741 ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat
     1801 aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg
     1861 atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg
     1921 gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc
     1981 gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg
     2041 cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg
     2101 acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt
     2161 ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa
     2221 ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg
     2281 ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt
     2341 gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa
     2401 gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa
     2461 aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct
     2521 gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga
     2581 acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata
     2641 cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa
     2701 gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta
     2761 tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg
     2821 gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa
     2881 gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta
     2941 gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg
     3001 ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt
     3061 taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa
     3121 tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg
     3181 gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca
     3241 cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga
     3301 gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag
     3361 attacaagta acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc
     3421 gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga
     3481 ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa
     3541 gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca
     3601 actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa
     3661 atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt
     3721 gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag
     3781 gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa
     3841 gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa
     3901 acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa
     3961 ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga
     4021 atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc
     4081 ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg
     4141 attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat
     4201 tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg
     4261 gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa
     4321 tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa
     4381 aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac
     4441 ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca
     4501 taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact
     4561 tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta
     4621 tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac
     4681 atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac
     4741 aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc
     4801 agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac
     4861 tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg
     4921 tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac
     4981 agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg
     5041 tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt
     5101 aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta cttattggga
     5161 agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga
     5221 agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc
     5281 ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta
     5341 gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac
     5401 tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact
     5461 aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct
     5521 ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat
     5581 gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc
     5641 gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat
     5701 gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac
     5761 gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta
     5821 ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg
     5881 aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc
     5941 gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga
     6001 gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca
     6061 gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg
     6121 ctatgtggcg caagcatgtt acagattggt acagagcttc ataaagaagg gccggaaatt
     6181 ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt
     6241 gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga
     6301 tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg
     6361 tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg
     6421 caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg
     6481 atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac
     6541 cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg
     6601 gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag
     6661 gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag
     6721 aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata
     6781 ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg
     6841 gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg
     6901 ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga
     6961 aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg
     7021 ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga
     7081 gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa
     7141 gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg
     7201 aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt
     7261 tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga
     7321 atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca
     7381 gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg
     7441 ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc
     7501 tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca
     7561 gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg
     7621 aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt
     7681 atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa
     7741 aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat
     7801 ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat
     7861 agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc
     7921 cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag
     7981 taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc
     8041 ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc
     8101 taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc
     8161 tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga
     8221 aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa
     8281 aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga
     8341 acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga
     8401 caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct
     8461 aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat
     8521 actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga
     8581 tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa
     8641 gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc
     8701 tgagtcggtg agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca
     8761 cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt
     8821 ttaggaaatt tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg
     8881 aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg
     8941 tttctgttat atttcgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc
     9001 tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt
     9061 tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa
     9121 tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg
     9181 gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa
     9241 gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg
     9301 atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct
     9361 atccatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt
     9421 tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga
     9481 aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt
     9541 tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg
     9601 accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc
     9661 gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg
     9721 ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa
     9781 tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg
     9841 cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag
     9901 aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa
     9961 tggagcgagg cgtaaaatat tttccaaaag atcaattgta caaagaattg aaaagacgtt
    10021 cagaaaatca agatccattt tctaacgaac gatattatga acggttttta gaggaaatag
    10081 ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat
    10141 atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat
    10201 gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc
    10261 gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac
    10321 gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa
    10381 tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga
    10441 aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga
    10501 ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg
    10561 gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat
    10621 ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga
    10681 aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga
    10741 cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt
    10801 ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt
    10861 gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga
    10921 agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc
    10981 aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt
    11041 gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa
    11101 agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga
    11161 taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga
    11221 aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc
    11281 aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata
    11341 ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat
    11401 tagtctcagg cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg
    11461 gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag
    11521 caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc
    11581 gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct gctggattca
    11641 cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa
    11701 tcatcaattg actataaaat catctgagca aagataaaga cattaacact actgaaaaaa
    11761 acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag
    11821 aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg
    11881 ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga tctctcggct
    11941 tatcctacga gtattattcg atacatttat atcaataatc ctttttttat tttatctgca
    12001 gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt
    12061 ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa
    12121 tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga
    12181 cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg
    12241 taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt
    12301 tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg
    12361 tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg
    12421 taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa
    12481 ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa
    12541 caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt
    12601 attttatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag
    12661 caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat
    12721 cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat
    12781 aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac
    12841 aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca
    12901 tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt
    12961 gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat
    13021 aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat
    13081 ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa
    13141 tacataggga tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc
    13201 agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac
    13261 tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc
    13321 cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata
    13381 agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa
    13441 taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg
    13501 catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa
    13561 gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac
    13621 aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa
    13681 catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag
    13741 ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat cactcctatt
    13801 ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt
    13861 cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata
    13921 aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa
    13981 gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt
    14041 cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat
    14101 catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat
    14161 cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt
    14221 cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg
    14281 tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta
    14341 ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga
    14401 tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg
    14461 cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta
    14521 aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt
    14581 ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt
    14641 ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag
    14701 attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg
    14761 tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat
    14821 cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat
    14881 gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc
    14941 ctttttggct ttcatgatac agttggttca agcaagtagc gagctttcca cttcctgggc
    15001 caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac
    15061 catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat
    15121 agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac
    15181 tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta
    15241 accgcaagat atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa
    15301 tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt
    15361 tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta
    15421 acttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg
    15481 ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc
    15541 cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt
    15601 cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat
    15661 gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat
    15721 aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg
    15781 gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag
    15841 cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg
    15901 gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa
    15961 ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag
    16021 aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag
    16081 tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaacgc
    16141 ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat
    16201 gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga
    16261 tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc
    16321 aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca
    16381 ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat
    16441 cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt
    16501 tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta
    16561 tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg
    16621 tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg
    16681 attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt
    16741 actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca
    16801 gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga
    16861 aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat
    16921 ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg
    16981 aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag
    17041 aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca
    17101 cagaagaaat ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa gtaactccga
    17161 cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt
    17221 tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag
    17281 aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag
    17341 aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc
    17401 tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt
    17461 caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg
    17521 gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg
    17581 aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc
    17641 gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca
    17701 cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg
    17761 ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg
    17821 aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg
    17881 atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa
    17941 cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac
    18001 gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt
    18061 cctgattgga taagttggat taccagatgt cattcagata attctgttcc aaaggacttg
    18121 cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat
    18181 aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta
    18241 gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc
    18301 agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa
    18361 ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca
    18421 tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa
    18481 aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt
    18541 acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct
    18601 attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc
    18661 ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa
    18721 ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca
    18781 acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca
    18841 atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc
    18901 gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc
    18961 acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg
    19021 gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc
    19081 attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca
    19141 taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc
    19201 ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt
    19261 gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt
    19321 gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc
    19381 gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg
    19441 atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat
    19501 ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta
    19561 atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa
    19621 ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg
    19681 gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat
    19741 gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg
    19801 ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca
    19861 gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc
    19921 gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc
    19981 gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt
    20041 cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat
    20101 tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct
    20161 tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta
    20221 tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa
    20281 gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga
    20341 gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc
    20401 ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca
    20461 acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt
    20521 gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat
    20581 gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga
    20641 aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt
    20701 aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc
    20761 atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta
    20821 ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt
    20881 gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa
    20941 aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg
    21001 gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt
    21061 catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc
    21121 atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt
    21181 taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc
    21241 aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac
    21301 aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca
    21361 gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt
    21421 ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc
    21481 ttttttattt taggaattag agagggattt gtccttctat gattttttgc tataataggg
    21541 aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca
    21601 aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt
    21661 gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta
    21721 taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa
    21781 tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct
    21841 gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat
    21901 gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat
    21961 actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag
    22021 aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca
    22081 ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg
    22141 aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc
    22201 gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt
    22261 gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga
    22321 aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt
    22381 aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt
    22441 cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt
    22501 aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat
    22561 acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc
    22621 attgactgga atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc
    22681 tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac
    22741 gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt
    22801 agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata
    22861 cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt
    22921 tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac
    22981 tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca
    23041 gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat
    23101 tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa
    23161 tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg
    23221 aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat
    23281 atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat
    23341 taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa
    23401 gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa
    23461 cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt
    23521 tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga
    23581 gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa
    23641 cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa
    23701 gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg
    23761 tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta
    23821 tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc
    23881 gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt
    23941 tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg
    24001 tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt
    24061 agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga
    24121 aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc
    24181 catttcagcg caataattcc ttttattcaa aaaacatacc aaaaata
//
LOCUS       DASJRQ010000040        24132 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJRQ010000040 DASJRQ010000000
VERSION     DASJRQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.040"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..506)
                     /locus_tag="U4A80_001407"
     CDS             complement(60..506)
                     /locus_tag="U4A80_001407"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1975036.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(517..2730)
                     /locus_tag="U4A80_001408"
     CDS             complement(517..2730)
                     /locus_tag="U4A80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1975037.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(2963..3727)
                     /locus_tag="U4A80_001409"
     CDS             complement(2963..3727)
                     /locus_tag="U4A80_001409"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1975038.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3728..4675)
                     /gene="prmA"
                     /locus_tag="U4A80_001410"
     CDS             complement(3728..4675)
                     /gene="prmA"
                     /locus_tag="U4A80_001410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1975039.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4688..5176)
                     /locus_tag="U4A80_001411"
     CDS             complement(4688..5176)
                     /locus_tag="U4A80_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1975040.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5142..5813)
                     /locus_tag="U4A80_001412"
     CDS             complement(5142..5813)
                     /locus_tag="U4A80_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1975041.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5951..7237
                     /locus_tag="U4A80_001413"
     CDS             5951..7237
                     /locus_tag="U4A80_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1975042.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7309..7503
                     /gene="ssrS"
                     /locus_tag="U4A80_001414"
     ncRNA           7309..7503
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4A80_001414"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7581..7865
                     /locus_tag="U4A80_001415"
     CDS             7581..7865
                     /locus_tag="U4A80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975043.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7938..8417
                     /locus_tag="U4A80_001416"
     CDS             7938..8417
                     /locus_tag="U4A80_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1975044.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8640..8783
                     /locus_tag="U4A80_001417"
     CDS             8640..8783
                     /locus_tag="U4A80_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1975045.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9361..10944
                     /locus_tag="U4A80_001418"
     CDS             9361..10944
                     /locus_tag="U4A80_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1975046.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11381..12565)
                     /locus_tag="U4A80_001419"
     CDS             complement(11381..12565)
                     /locus_tag="U4A80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1975047.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12590..13597)
                     /locus_tag="U4A80_001420"
     CDS             complement(12590..13597)
                     /locus_tag="U4A80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1975048.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13769..14128)
                     /locus_tag="U4A80_001421"
     CDS             complement(13769..14128)
                     /locus_tag="U4A80_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975049.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14100..14471)
                     /locus_tag="U4A80_001422"
     CDS             complement(14100..14471)
                     /locus_tag="U4A80_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1975050.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14488..14790)
                     /locus_tag="U4A80_001423"
     CDS             complement(14488..14790)
                     /locus_tag="U4A80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975051.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14807..15277)
                     /locus_tag="U4A80_001424"
     CDS             complement(14807..15277)
                     /locus_tag="U4A80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1975052.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15252..15530)
                     /locus_tag="U4A80_001425"
     CDS             complement(15252..15530)
                     /locus_tag="U4A80_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1975053.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15527..16576)
                     /locus_tag="U4A80_001426"
     CDS             complement(15527..16576)
                     /locus_tag="U4A80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1975054.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16533..17462)
                     /gene="tadA"
                     /locus_tag="U4A80_001427"
     CDS             complement(16533..17462)
                     /gene="tadA"
                     /locus_tag="U4A80_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1975055.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17594..19348
                     /gene="ade"
                     /locus_tag="U4A80_001428"
     CDS             17594..19348
                     /gene="ade"
                     /locus_tag="U4A80_001428"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1975056.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19446..20108)
                     /gene="trmB"
                     /locus_tag="U4A80_001429"
     CDS             complement(19446..20108)
                     /gene="trmB"
                     /locus_tag="U4A80_001429"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1975057.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20178..20960)
                     /locus_tag="U4A80_001430"
     CDS             complement(20178..20960)
                     /locus_tag="U4A80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1975058.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21164..22375)
                     /locus_tag="U4A80_001431"
     CDS             complement(21164..22375)
                     /locus_tag="U4A80_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975059.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22368..23105)
                     /locus_tag="U4A80_001432"
     CDS             complement(22368..23105)
                     /locus_tag="U4A80_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975060.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23243..23677
                     /locus_tag="U4A80_001433"
     CDS             23243..23677
                     /locus_tag="U4A80_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1975061.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23670..24014
                     /locus_tag="U4A80_001434"
     CDS             23670..24014
                     /locus_tag="U4A80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1975062.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         7175 a         5083 c         4303 g         7571 t
ORIGIN      
        1 ttatcagtct aacttaagcg acgtgtttca tagtttttac tagtgaaaac cagattactt
       61 tatttgtttt tagaatcaat aatgatcgtt acaggtccat cattgacgag agaaaccgac
      121 atatctgctc cgaattgtcc ggtagcgact gtgatgccgc gacttctgat tccttcattg
      181 aatgattcgt ataaaggaat agctgtttct ggtcgtgcgg cttccacaaa gcttggtcga
      241 tttccttttt tcgtatccgc aaacagtgtg aattgagaga tacttaaaat ttgtcctccc
      301 acatcttcta cactaatatt catttttcct tgttcatctt caaaaatacg catttgtgca
      361 atttttttta ccagatagtc cacatctttt tgcgtatcgg tttcatgaat gcctaataaa
      421 acaagtaggc cgtagccaat ttttccaatt tcttgctgat cgactgacac agcagcttta
      481 cttacccgtt gaacaactgc tctcatatct gtcctcctat ccattcgtcc ggcgtacact
      541 gtagacatca ggtactgctt tgattttatc cacgatactc tttaggtggg ataaattctg
      601 gataccaacc gtcaaatgga tcgtcgccat tttatcttta ttcgtccgtg cttcaacgct
      661 gttgagattt ttcgttagcg cattgactgt ttgaagtaca tcattcaata agcctgaacg
      721 attatagccg taaatttcca aatctgcatc atactctttt cgtgtattcg atgtatcttc
      781 ccattcgact tcaatcaaac gttctgctac atttggtttg tcaggctgaa cattcggaca
      841 atctcgacga tgaatggata tccctctgcc tttagtgata taaccaacga tatcatcacc
      901 aggaatcgga ttacagcagc gactgatacg aattagtaag ttttctaccc cttgaatgac
      961 aacgccacct tcatgacgta ctttcatttt gtcaggttct tttttcggct gattcatgat
     1021 ttcttctgct tcttgctttt gctgctcgat tttttgttct ttacgttctt tttcagtcag
     1081 acgattggcc atcgtcaagg ggctaacttc tccataccct acagctgcat agagatcatc
     1141 ttctgtttga taattaaaac gatcgagtgc ttcttgcagt ttgttcttcg tcaaaatatc
     1201 tttaggcgta aatcctagat ctgtaataca tttgaccacg gattcgtggc ctttgatcac
     1261 attttcttct cgatcttggg ctttgaagaa acgtttgatc ttatttcttg ctttgctagt
     1321 agcaactaat ttcaaccagt cgcgacttgg gccaaatgaa tttggagaag tcatgatctc
     1381 aatgatatct ccgtttttca atttgtaatc aagctgcacc attttgccat ttacttttgc
     1441 accagtggtt ttgttaccga tatctgtatg gatactgtat gcaaagtcca atggtccgga
     1501 tccttttggt aactcagtaa cgtctccttt tggtgtgaag acgtagactt tatcactaaa
     1561 gatatctcct tttacgcctt ccataaattc agaagcatca tagctttcgt cttgaagttc
     1621 gagtatctca tggaaccagc ttaattgttt cgtcatacca tctggttcta ctttttcatt
     1681 ttttccttct ttataggccc agtgtgcagc aaccccgaat tcagcgattt catgcatttc
     1741 ctgtgttcgg atttgaattt ctaccggatt tcctgccgga ccgatcacgg tagtatgcaa
     1801 agattggtac atattagctt ttggcatggc aatataatcc ttaaatctgc cgggcattgg
     1861 tttccatttt gtgtggattg ctcctaatac agcataacaa tccttgattg aatctacgat
     1921 cactcgaata gctagcaagt cataaatttc gttgaactgc tttttctgat ctttcatttt
     1981 acgatagatt gaataaatgt gttttggccg tccatagatt tctgcaaaaa tccccagctc
     2041 ttctgtcgct attcgaatat cttcaacagt accacttacg tatttttcac gttcttctct
     2101 cttcgtctgc atcaagtgga cgatacggta atactgtttt ggatttagat aacgaagtgc
     2161 tgtatcttct aattcccatt tgatccggct gatccctaga cgatgtgcaa gaggcgcata
     2221 gatttctaaa gtttcctgag cgatccttct ttgcttatct tctcgcaggt gcttcaacgt
     2281 acgcatgtta tgtagtctat cagctaattt gaccatgata actcgtaaat cttgagccat
     2341 tgctagcagc atctttcggt ggttttctgc tagctgctct tcgtgggatt tatattttat
     2401 tttccctaat ttggttacgc cgtccactaa catagcaacg tcatcgccaa attcttctct
     2461 cagatcttct aaagtgattt ctgtatcttc aacaacatca tgtaagaagc ctgtagccac
     2521 tgtatgggga tccattttta aatctgctaa tatacctgcg acttggatag gatgaataat
     2581 gtagggttcg cctgattttc taaactgtcc gtcatgtgca gcagtcgcgt attcgcatgc
     2641 tttctcaaca aatgctacat gttcgggacc catgtattga ctaacgagct ttatgacacc
     2701 gggtcccgtt aaaatttctt cttttgtcac aggaatcgat ccccctttct tttttcggta
     2761 aaacgggaat cgggacaaat atctgtttga taattgtccc aaaatccgtg cagttatacg
     2821 tttttttacg gaaatgttcg ctttttaaaa gctttttcaa acgatttttc gtatccattg
     2881 tataaataat tgtttctatt atacaaattg ttgactattt tttcaattct ttctgtcgat
     2941 actcttttat taatgaagaa cgttagctga aagaaggatt cattaattcc cattgatagc
     3001 tagcagcact taataaataa agaggtgctg tttctgtacg aaggattctt ggtcctaatc
     3061 cacaaagtat ccctttgtgt tctacaaaat aatcgacttc atcgcgggat aatccacctt
     3121 ctggtccaaa gacagctaag attcgactat ctggttttgt ttcactaaga attttggctg
     3181 catttgattt ttcgccttgc tttgctgatt cctcataagc aaccaagacc acgtcataat
     3241 catctagccg ctcaaataaa tcttgtttcg tttccaataa ctctactgat ggcacgatct
     3301 gccgatggga ttgttccgct gcctcttgag caatcttttc aagccgttgt tgtcgtttca
     3361 tacgtttttt tgcatcccat ttaacgacag aggctgatgc cgggaaacca agaaaagctt
     3421 gcgctcctaa ttcagtggat ttctgaacga tccaatccag cttttcccct ttaggatagc
     3481 cactcgctat ggtaatcgaa cttggcagtt ccttttgatt ttcttctttt ttaatttctt
     3541 tcagataaac agcctcttca gtaatctccg tgatttctgc aacaatggct atttgatcat
     3601 tgaatgccaa aaaaacttca tcctcgggtt ccatcctcat aacccgtaca atgtggtgat
     3661 agttttcccc ttctaaaaca aaatagtctt ttgattcata tgattctttt aaaaaatacc
     3721 gctgcatcta ttccacctct ggttttttca aaataattgc ataccagtct ttttgctgga
     3781 aaatttgatc cacgataaac ccttgttcta acatggcatc cagaaccatt tgcttcttct
     3841 cttcaatgat tcctgaaacg atcagcgtgc ctgtttgctt cagtaaacgc caagcatctg
     3901 ggatcatcaa taagatgata tctgctaaaa tgttcgcaac gattacatca ctttcaatct
     3961 cgattcctgt caaaagatcg ttagctgata catggacatc tttggctatt tcattcatat
     4021 ccatattttc tttcgccgaa cgaacggcta cttcgtctaa atcaaaggca tagacatctt
     4081 ttgcaccaag atattttgct gcaatactta gcacgcccga cccagttccc acatctagca
     4141 atgtttcacc accacgcaag actgtttcta gtgctgtcaa tgtcaaggaa gtcgtgggat
     4201 gagtgcctgt gccaaatgcc ataccaggat ctagtgtgat aattttttct agaggatctt
     4261 ctgtctggta gttttcccag cttggcgcaa ccgtcagcat tcgcgtaata cgcaccggat
     4321 gatagtattt cttccaggct gttgcccagt tgctttcctc tacttcacta actgttactt
     4381 tattttttcc aatagctaga ccaaactcag gtaattgtgt gatacgatct ttgataaaag
     4441 gcaagatttc cggtaagaaa gtcgtttccg gaaaataagc catgactagt gcgccttctt
     4501 tcagcgatgt aaaatcattt ttatctaaaa tctcaccata ttgatccgac tgaaaatgtt
     4561 caacatctaa agaatcttca atcgcgacac cactcgcccc tgcctccatc aaaatattag
     4621 caactgcttc gactgcttca ctttcagttt ctacttttac ttcattccat ttcatatctg
     4681 tcactcctta ataacttggg tacggtaaat aagtatctgc ttcattttgc tgttttactt
     4741 ctactaattc tgctagttgc tgattatccc agtgaagctc gaatacagct tcgtcatcgt
     4801 cttcatccaa aaaatcaagc aaatcgcttt gcccttgagc taatacttca tttagatatt
     4861 ccactaaaga atcagctact gattttgcca accctttttt tccttcataa gggattactg
     4921 ctagataatt gcgttcatca aaaacagatt tctgaggatt aaaaagaagt acgccgtctt
     4981 caaattcgat aattggttct tgcgaaacga tcccttctgc atcatcaatc tcaaattgtg
     5041 ctttattttc tgcaaacaag cgaacaacga tctcgatcgt atgatttttt gtatcccaat
     5101 ctaaagctac atcatagtct gtcacttgtt tttcaattat ttcatctaaa tatgttaata
     5161 gatccttctt tttcattttc tttcctccaa ccccaatctt tctcgatttg gttttttctt
     5221 tgtaaagaga tataaggatt tcccgacaca acaaaacgca acggcttttc tgtccataca
     5281 cctttgtttg ggataccgat tctaggcaaa gcattgatct tttttggtgt cttttttttc
     5341 tcaaaaacta agcgtaacga actatctgct atagactgac cataaagttc ttgaggaatt
     5401 gctaatgctt ccactaattt tcccggaccg ttgctgatat ctggaccagt ttttccgcca
     5461 cgatttttac tcatttgatc aatcattgcg gcgggttcga ttgcacgtat cattactcct
     5521 tgaggaatcc cttctggctg agtaatgatg ttcaaaatcc gatgagtatg catcgtgtaa
     5581 aggtagatcg tacctggttt ttcatacatt gcccgcacac gcggtgttct tcttaaacca
     5641 tagctatggg cggcctcgtc ttctggacct aggtatgctt ccgcgtctac aatatatcca
     5701 gctaatcttc cttctggtgt gtcatgctcc aaatacatcc ccaataaatc ccgtgccact
     5761 tctggcgtcg ttttattcca aaaaaattca tctatctttt gtttatccgt catttttctc
     5821 acctacttta tcatacacaa tattagttgg ataagctatg aaaacaaaac aacagaaggc
     5881 tgttctttct aggcatcttg ctaaatatga tataattaag ggacgtaaaa acaagaagga
     5941 ggaaaatcct atgcaacagc cattggctta tcgcatgaga cctcgaacga tcgatgaagt
     6001 cgttggtcag cagcatctag tcggggaagg aaaaatcata cgccgcatgg tcgatgctcg
     6061 catgctctct tccatgattc tatatggacc tcctggtact ggaaaaacga gtatcgcaag
     6121 tgcgatcgcc ggctctacta attatgcatt tcgcatgcta aacgccgcta cagattcaaa
     6181 aaaggatcta caagttgtag cggaagaagc aaaaatgagt ggtacagtga tcctcttatt
     6241 ggatgaagta caccgattag ataaaacaaa acaggatttt ctgcttcctc atttagaaag
     6301 tggccgaatc atcttgatcg gagcgactac tgagaatcct tatattacca tcaatccagc
     6361 gatccgcagc cgtacacaga tctttgaagt caaaccatta acagaacaag acatccagtt
     6421 ggcagtcgaa catgctttaa gggataaaga acgcggcctt ggacaacaag caatccagtt
     6481 agacgaagat gctcttcttc acctttcgcg tgcaacgaac ggcgatttgc ggagtgcatt
     6541 gaatgggctg gaactggcta cactatcgac tccttcagat aaagaaggaa ggattcatct
     6601 tacactgtcg atcattgaag aatgtgtcca aagaaaagct cttacccatg acaaaaatgg
     6661 agatgctcat tatgacgtta tttcggcttt tcaaaaatcg attcgtggaa gtgatgtgga
     6721 tgctgcactt cattatctcg cccgtttagt tgaggcaggt gatctggcaa gtatttgccg
     6781 gcgtctgatg gttatcggtt atgaagatat aggtttaggt aatcctgctg ctgcggctcg
     6841 tacagtgaac gctgtattag ctgctgaaaa attgggattg cctgaggcac gtatcccact
     6901 agcagacgtc gtcgtcgatc tttgcttgtc gccaaaatcc aattctgcct acatggcatt
     6961 agatgcagca cttgctgata ttcgagaggg caaagctgga gatgttcctg atcatttgag
     7021 agatagccat tacaaaggtg cgaaatcact caaccgtggc gtgggttatc agtatcctca
     7081 tcattttgat caggcttggg tcaatcagca atatttacca gataaactga agaatgctca
     7141 gtattatcag ccaaaagata cagggaaata tgaacaggct ctgggtcaac agtattaccg
     7201 tataaaagag tggaaaaagc atcctccaaa aaactagaca agaaaacggt tctgtttctg
     7261 cgagctctct cattgctctt tttaaattca tatgctacta tagaggtggg aaatctgtga
     7321 tgttcgaatt gtcctctttg tgtgttgacc gaacattttt attgatatct tgggacccgt
     7381 cttgtgtcac tgctggtaac atgccttatc atttggtagt tcgaagctac gaccatggga
     7441 cccacctgct tgaattcgcg ggatcaatac tacaggacaa acaacggcat taacggagat
     7501 taccatcttt taaagaataa agcaatttgc tcttggcaaa ttgctttttc tatttgttgc
     7561 ttattttgga ggatttgttc atgattagga ttttaatgat tatcgccact ttagtattat
     7621 tattcgttag ctattattta tttaataaac aggatatctt ttttgtattg atcaacaaaa
     7681 atgataaaaa tcaaggattc ctacaatttt acggagctgc atatgctgtt ttaggcgtaa
     7741 tgggtatttt aacagctttt ttcaatcaac gctttattgc cttggtcttt ttactgatcg
     7801 taattattgt ttctgccacc ttcagtatcc gatttgcaaa aaaaattgct gaaccaaagc
     7861 agtaaataat tctctttttc cctaaagtgt tctacaataa aagagtacaa tacgattgac
     7921 ttgaggagtg aatgcttatg ttacaacaat acaaaaaaat catggttgcc gtagatggtt
     7981 ctgatgaagc tgaattagct tttaaaaagg cggtcaatgt agctatccga aataacggag
     8041 aacttctatt agcacatgtc attgacacac gttctttcca aaccgtttca tcctttgatg
     8101 gaatgcttgc agaacaagct actgaaatgg caaagcaaac attggcagat tatgaaagca
     8161 atgcgaagaa agctggcttg aataatgtaa cctctgtcgt agaatatggc tcaccaaaac
     8221 aaattattgc cagagaaatc ccagaagata atcaagtaga tttgattatg ttaggagcca
     8281 caggattgaa tgctgtagaa cgtttattta ttggttctgt ttctgaatat gttattagaa
     8341 acgcagcttg cgatgtgctt gttgtccgaa ctgatttaga aaaccaattg ccagctaaag
     8401 accacaccaa agcttaacac gtagacgacc tgccaactat cggcaggttt ttttgcacaa
     8461 aaaaccgtga taaaaacaaa agacgcggca agcactcatc ggtctttata atgtttaaaa
     8521 taaagggaaa acagtcaatg agttcaagta aaataaaaaa aagtgaacac cagggcgatt
     8581 agtcgccctg gtgtggttta aaacgaccta agcatgccaa agcttattgg ttcgtcaatg
     8641 tggaagtttc cgatgaatct gatacgaaaa aatatgaagt atcgatggcg gataccgatt
     8701 ttatcgccaa actaaaattt tatttcgggt tcgggtaccg cgagaaggca agtgctacat
     8761 tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg ccattgcaac cacaacgata
     8821 ctcctgaacg cgatgttggt gagatttcca aagagaagag ctgtttaatg caacggtatt
     8881 aagcaaatgg caaaaggaag tcatgtagac aaagtttttt atcaaacacc atatgatttc
     8941 gcctgaacgc tggtttagct cagaatacaa tgatgtggtc cacgagacat taccctcagt
     9001 cagataaaaa aagctctctg acagaattac acatcgataa atattaagta aatgtcagcg
     9061 ctagttgaac tttctgacac tttcaggtac actggaaaag acaactacac gaaggaggga
     9121 aaatactatg atgcgcatga ctaaaataac aagtaatgaa aattaccaac gataccatat
     9181 cgcatttagc catgcaattg gttttacagt tgctgttgac gggacaagtt tgtccaaaac
     9241 agttgaattc acgtaaaatc aatggccatc tcaaggtatt tttctcatga taatctgagt
     9301 ttaaataaca caggccatgg ataaccatgg cctttttgag tggtctcaag gagagagatc
     9361 atgatcatac aagcaatttc tattaaaaaa acaatcggcg ggaatccatt atttgaggat
     9421 ttatcctgtc aaattgaagc tggtgaaaaa attgccttag tcggtatcaa cggaactggc
     9481 aaatcaacct ttttacaaat cttgaccggc agagaaggtg tcgacagcgg tgttatcagt
     9541 cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac ttacagtttc cgaagcgacc
     9601 gtcaaccact acttgctcca ttctgccgct gagatacagg acctcaaaca gcaaatgagt
     9661 cgctacgaag cattgatgac agaaccagat acaaacttag agaagtgtct ggcccgttac
     9721 ggtgagctgc agcatcgctt tgaagaacta ggcggttacg tattagaaga ccggattgca
     9781 gcgatcttgc aaggcttagg gattccacac cgtcaggaag cacggttaac agaacttagc
     9841 ggaggcgaac gagttaaagt cgctttagcc aaaattttag cagccgatgc cgatttactt
     9901 ttactcgatg aaccgaccaa tcatttagat cttaacagta ttcgctggtt ggaaaactat
     9961 ttacagaata caaaaaaatc ggttctggtc gtttctcatg atcggcaatt tctcgatcaa
    10021 gtcaccacaa aaacatggga actggaagaa ggtgcattga ttgagtatcc cggaaactac
    10081 agccgttttc gagtcttgaa agaagctcgc ttggctgagc tgacaaaaaa ttacgaactg
    10141 cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc gttttcgtca gtgggcacat
    10201 gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac tggaacgccg cctggcgaaa
    10261 ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc tgcaaagctt gtctaatggt
    10321 ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc accagcagta cgctgatcaa
    10381 gtactattca aagacagcag ttttgcagtc tatcgtggag atcatttagc catcatcggc
    10441 gacaatggtg caggaaaatc aaccttactc aaattattac tcggtgagga aaagccttac
    10501 ggtggcacca tcaaattagg tagtagcttg caaatcggtt atttgccgca gcagctgcag
    10561 tttgccgatc ccgatagccg tttgctggct tacgccatca cattgactgg aaacgaagaa
    10621 gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa gcgatgtggg taaacggatc
    10681 aaagatttat ccggtggtga aaaaatccgt ttggcgttgc tgaaactctt tttagaaaaa
    10741 atcaacgtcc tgattttgga tgaaccaacc aatcacctcg acagctatgc tcgagaagaa
    10801 attgaagaaa tgctgcaaga ctatcaagga acgctcctag cagtcagcca tgaccgctac
    10861 tttttgcagc aacacttcca agaagcactc gttattgatc aggagcagat cagccgccag
    10921 ccgctcggcc aactgattga ctaggacgtt ttttcgcaac aaaaaacttg attttcatac
    10981 ctgtgctttc acgattcagg caaagtacag gtggaaaatc aagtttttat tattcgatca
    11041 atgaatgttt cggatcggtt ttctagctaa acataaagcg aagaataaag tcgacagccc
    11101 acgaggctga tctaaaaatg ttttttcttt ttacatatgt aatgtccctt ataaagatga
    11161 atgaaaataa aatattggga ttttcctgaa aatcaataaa taaaatgcca gtcttttccc
    11221 ttttgaccac ttttccaagc aaaattccct aagaagatcc tgtaaaaaag aagggaaatt
    11281 gaagttaaat taccattttg cggtcttctc cgcaaaaaaa acacatgcaa gaattccctc
    11341 ttgcatgcgt ttcatctttt atcctaacga atgctttggc ttagccgcgc agacgctcca
    11401 catcacgggc aatcattagt tcctcatctg ttgggatcac taatactctt acttttgatt
    11461 cttttgtaga gatatctact tcttcaccac gcaattcatt caacgctgga tcgatttcac
    11521 aaccaaacca agacatacct ttgatgattt cactacgtac atgtccgtca ttctcgccga
    11581 taccagcagt aaagacaatt gcatcaacac cattcatcgt tgtcacataa ccgccgatgt
    11641 attttcggat gcggtccacg aaaatgtcat aagctaaacg aatatgttct ttttcatagt
    11701 tcttttcaag atcacgcata tcactagaca atcctgttag gcctagcaga ccagatttct
    11761 tgttcaaaac attgatcatt tcatcaatat caatctctaa tttttccatt aaatatggga
    11821 gaacagctgg atcgatatca cctgaacgtg tacccattgt caccccagct aatggtgtga
    11881 atcccataga agtatccatg gatttacctc catcgactgc tgtgatcgat acaccatttc
    11941 ctaagtggca agtgatgatt tttagttctt cgatcggttt gttcaataat tttgccgcac
    12001 gttccgacac ataacgatga cttgtgccat ggaagccata ttttcttact ttgaaatctt
    12061 cataataaga caccggtaag ctatataaat aattttgctt tggcattgtt gagtggaaag
    12121 atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat ctttttaaac gctttgatcc
    12181 ccattaagtt tgcaggatta tgcaaaggag caaattctgc taattcagca atgcggtcca
    12241 ttactttctc atcgatcact actgaatccc cataagtttc tcctccgtga acaacacggt
    12301 gccctactcc ggtgatttca tcataagagc ttaaaatttg cagttcaatc aactgatcta
    12361 acagcatttt tactgctact tcgtggtttt cgatatccat gatttgttcg aatttttgat
    12421 tatcgccgta cttgatggta aaaatagaat cttgtaaacc gatacgttcg acgatccctt
    12481 tcgcaactac ggtttcttct ggcatttgat ataattgcca tttcaaactt gaactcccag
    12541 cattgattgc aattgtttta gacatatgac tctccttctc gttgaatgat tacagagcag
    12601 atgatttcca gtttttaaac tcgttgaaaa attccgtcac cttctctggt tcttttaatg
    12661 aagcaagttt cacaagcaag acttctttta cttgttgtgc ttgtggtcct tttttctgta
    12721 agatcaaaat acttttttgc gattgtttat tacggaacaa ttcatctggc aattgaatca
    12781 ttccttgaag ataaccttct tctttaaacc attttttgat ttcttcactt tgatccgttt
    12841 ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc cggcttcaca tatttcattg
    12901 attgctctag caataaatga tgtgcataac tatggccttc ttcggtactt gttaaaaact
    12961 cttttgcttt ttcgtcattc ggataataac cgattggcaa atcgctgatt gcaaaatcga
    13021 caggatctat taataattcc tgcaacccgt cttgatgaaa gtattgaaca tttgcttgtg
    13081 tcaaatcact tgtagaagct gcgacagcca acaatgtatc atcaatatcc acaccaattc
    13141 cttctgtttg gtaccccgca ttcgataggt ttaaaagaac tgttaataac aagttgccca
    13201 ttcctgccgc aatatccaga atcttaaccg gtgctttttt atttgtgtat agttgttcaa
    13261 taagaaaaac aaataaaaaa ccgatactat caggagtgag ttgatggttc gcctgcaaat
    13321 gttctgtctg gctgcccttc aataaaagta attgagacag tctgcgccat tcttctggct
    13381 cgaaagaaag tttcttcaac tcttcataaa gagctgtgat ccgttgcgtt gtctcttttg
    13441 tcggcactcc atccaccaca cgtacttgat aatcatctat taaattttct gcattttcga
    13501 cataggcttc taaaaaggaa gttcccaatg cattctgtaa aagctgaata gcttccaggt
    13561 tttgattaaa cccttgttcc attttttctg gtaacatggt gcacctcatt tctagatata
    13621 ttataacaat tttttcacct gtttaattct atcgaacaat tgacaaaatt tcaatcgttt
    13681 tcactgtttt ttatttgtga aacggcaaac atttttttat taaaaacagt gttataacag
    13741 aaaagagggc gttttcacga acattccatc attctgtact agtatctgta tcatttttta
    13801 cttcttctgt gaaccgaaag ataaacggtg agacttccac ctctaagaat agggactgtt
    13861 gtacccactg atatgttaca gatccagttg tgtagtaaaa aactccttcc gaactttcgg
    13921 caagcgtaga ctgttccgat aaagccatct gcttcattat ttttgctata taataatttt
    13981 tgattcgtat attaagatcc attgacaaac ggtagctttc taatgctgcc atcaataaaa
    14041 acgtacataa aagaaataaa agcaagacag aaaataaaat agagccctta taacattttc
    14101 tactgatcgt tttttgtcca tatggcatat ccatcttctc cattttcaaa gtgtacataa
    14161 aaagaaattg tgtgttcttt ttccaataat tgaagctcag tgatacttgt taacataggt
    14221 tgatgtccgc cggaagttct tcttttacgt atcattgcct gatatctttc gatgatatac
    14281 tgtttatcct cgatcgtgta gtgcagttgc tgatttttca cagagacatt gtgactgctc
    14341 ttgagttctt cttctaattg tgctaaaaat attagccact ctttttgatc tttacgctgc
    14401 aaaaattcac ttacttggat ggtttgctga atcaaaagat tgaatgtgag caatagactg
    14461 ctcaatatca tcagtccaat tagacattca atcagcgtaa atgctggaat tacttgtttt
    14521 ttcaatactg atcgcttctt tctccatatt gacaaacact ttttcgatta ctccattatt
    14581 atcgacaatt tggatatcgt atgggatcat ttcttccctt cgctctggtc ttttgtgata
    14641 catccgctgc gtcttgatat cttcataaat cattcgaata aatagcagtt cctccccact
    14701 tttcttttct gcctgaagca aaaacagctg gccaaagctc actaagtaga cacagacaga
    14761 agcgatcata agcgctgtaa gggactccaa caaaacaaat cctgttttat tcaattcttt
    14821 ttgtatagcg accactccct atctggaatt gatacgtaat cgtccgctct ttttcttccc
    14881 aataaaattg ataagctttt agtgaacttt cattcccggt tctagcagca aaagtaatag
    14941 aagcatggcg tttcaaggta atctcatcag gaatatctaa tacttcccaa tttattttat
    15001 ctggattcgg aacatgaaaa atgatttgat tctcttcact attccaataa aagcctgttt
    15061 ggatctgatt cacaatagcg attttttgcg tagaatagat tcgtttttca aattggccga
    15121 aaaattgata gactgccaat tcattcttcc atgcagaaaa tgcgagaact ggaaaagaaa
    15181 ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa caacgtatat cctgatgcta
    15241 aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt ctcgtttgat atagccttca
    15301 ttcaacaatt cctccacagt aggaactctt ccattatttt gaagcgcata gagctctttt
    15361 tgtgtgtcta ccaactggat gatcgcttca cgactttctt ggtctacatg attcctataa
    15421 cgcgacaaat tagggacgaa caacaacact aatacactga tgatcagtaa gacgattagc
    15481 atttcgatca atgtaaaacc gctataataa gatatttttc tctttttcat ggcaaaacct
    15541 cctctatgct tccgtaaaca ggtaataaaa cagcggcata aactaataaa atcaaagccg
    15601 ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca gtgatgaaga cgtttgaaaa
    15661 gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt acctaaactt ccctttgcct
    15721 ctccctgtaa aacaatttta ctgaattctt ctgatagaaa acgataattc tggaactgct
    15781 cagccaatga catacctgcc tccaatcctt gaatcaactt agaggcaaga tgctgaatca
    15841 gactttcttg tttcgtttcc tttaagcaga ctatgatttg ccttaattcg aaaccttcat
    15901 gaaaaagctt tccaatctct agtgacaaat aagccgattg atacaaggaa tatatcggtc
    15961 caatcaataa ttgtctgctt atccattcac tctgatcaac aggagacaac ttcatcagac
    16021 gataccttac tagtaaagcc aatagagtaa ccgctaggat gaatagtaga acataccaat
    16081 gaaagacttg tatcagctga ataccccaat gctcttgcat gaccattcct gaaccaataa
    16141 gctgaggcaa aacgaacaac cgcatactaa acaagatacc tagcaaaaag gtgagcaata
    16201 gcatcggata tgcaagcagc tttttcaaat cattgcgaaa tccttcaaca agccgaaact
    16261 gtttggcaat ttccaacaaa gttgtcggca gattcccatg acgttcagct aactcgatct
    16321 gtaccagttg ctctgctcca tatcctattt gcgaaagtac ctcactaaac gcttgccctt
    16381 cctttagctg atgttggata tttgccaaaa taacaggtgg aaacgaacgg atcgtgagta
    16441 ataaataaag ggattcttgc agactaaaac cagataaaag cagttctccc atggtttgtg
    16501 caaactggtt ttgctggctt cttgttaatc ctttagaata gttctgactg ccaaatattt
    16561 tcctgagctt cttcataact ttcctcccat gattttttta ctgattcttc ctccataaat
    16621 tgataactcc ataaaacaga aggatggtga ttggcatctt ccaaaagttc ttgataaatg
    16681 acaccagcta aacattcaga tagctcttct tttttctttg tcagttcatg gattctgacc
    16741 aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa cccggtgccc tgttagtgct
    16801 gctcttaccg cagcatgtgc agttgattca tctcttatct cgccaatgat tagcaagtct
    16861 ggacgatgcc gtaatgcgag tctcaaaagt gtatcataag tctgtgctat tttcggattg
    16921 atctgtagct gcagaaaaaa cggttcttct atctctacag gatcttcgat agtaatcact
    16981 tgcaattttt cttccaaagc caaccgatac attagagaag tcttgcctga tccgactggt
    17041 ccgctgaaaa ggtataatcc acgtctgacg gttttctgta aaatagttgc aaaatctcga
    17101 gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata atagccgaat cactaaactc
    17161 tctcgttgaa ggtaatctcc tacagaagac aaccgcagac gaacttcctg tttttctaaa
    17221 gaataagtaa atgcacctaa ctgagctttt cttttttctc cgatatccat ctgtcccaaa
    17281 tacttaaatc ttgagatcag ttgctgtcca tctgccgaag agactttctt ccagaatgtt
    17341 cgtttttttc catagcgaaa atagatgtaa aatacttctg aagctggcaa tatatatacg
    17401 tcttgggcat gtttggcaaa actttcactt atcatttgat ccgataatcg ttttatttcc
    17461 attattctcc cctccacaaa aaacatacgt ctttttgatc tttttgtttc aaataaaatg
    17521 aatttacaaa aaaatattgg caaaaaccga atttatgtta tacttataaa aatttatctt
    17581 gggaggcact ccattggaaa caacattaag gaaatatatc gaagtagcaa gtaaaaaaaa
    17641 gcctgctgac ctagtgatca agcatgctaa gatcgtcaac gtctttacga aagaaatcat
    17701 ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga atcggtgaat acgaaggaaa
    17761 aagcgtacat aatgctaatg ggcaatatct agtgccagga ttcatcgatg gccatgtgca
    17821 tatcgaaagc tcacttttat caccaagcga attttcaaaa gtatccttgc ttcatggagt
    17881 tactactgta gtgacagatc cccatgaaat aggaaacatt gctgggagta caggtcttga
    17941 gtttatgata gaagatgcaa gacaaacact catgaatatc ttttttatgc taccttcttg
    18001 tgtacctgta accccttttg agaccaatgg tgcaaattta aatgcaaaaa aattagcccc
    18061 ttttttacaa aaacccgaag ttttaggctt agctgaagtc atgaactatc aagcagtagc
    18121 aaataatgaa acagacatcc tagaaaagat ccaattgatg caccagcata agaaaaaaat
    18181 tgacgggcat gcagctggta tcggcatgga agagttaaat gtttatcctg cagcaggtat
    18241 tcggacggat catgaagcta caactgcaaa agaagctaaa gaacgtttgg atcttggtat
    18301 gtatttaatg gttcgagaag ggaccgttgc aaaagatctg gcatcgctac taccggcgat
    18361 cacacctgaa aattcaagaa gatgcttctt tgtcaccgat gataaactga tcaatgattt
    18421 agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct attgcacttg gaatcgatcc
    18481 attacaagcc attcagatgg caacattgaa tgcagcagaa tgttttgatt tgaaaacact
    18541 aggtgcgatt gcccctgggt atcaagctga tttcttctta acagacgatc ttaccaagct
    18601 gccaattcat gaagttttct ataagggaac attagttgtt caatctgggc aactccaaga
    18661 ccatttattt gctgatcatt caaattcata tacgtgttgt ctgccaaaac taaacgctca
    18721 accattaaat ccacattcgt tagagctgct tctttcttct tcacaagccc atgtgatcga
    18781 aattatcccg aatagtctag tgactaaaga tttggtagaa aaagtggacc gacaaggcga
    18841 acgattcatt ccttcaatcg aaaaagacca attaaagatt gcggtgatcg agcgtcacca
    18901 tcaaacggga aacattggat taggcatagt gaaaggattt cagctgaaac aaggagcgat
    18961 agcgacaacg attgcccatg actcgcataa tctagttgtc gttgggacaa acgacgagga
    19021 tatgctgtat gctgctaatc ttttgattca aaaaggtggc gggatgaccg ttgtcaacaa
    19081 acagcaggaa atagcttgtg tttctcttcc tataggcgga atcatgacac aggaaccttt
    19141 ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa gcacatcaac taggagcagc
    19201 taaaaccttt gatccttttt tgacactttc tttcttaaca ttatctgtca tacccgaatt
    19261 gaagatcacc gacaaaggat tattttcatt ttctcgcttc caattgattc caccttgtgg
    19321 aaaaaccgat gaaataaata cattttaaat caaataaatc ccagaaaata cattacatct
    19381 gtaatgttca ttttctggga tttatttatg gatattaatg tttttatgaa agcagaaagt
    19441 tagtttcaca gacttttctt tttggtctta tcttgaaatt gagcttctac tcgataaata
    19501 cgttgacctt ttgaagaaaa tttttcttca tactccgtca taatattgcc ttcaaattga
    19561 ctttgatgta aatctaacca tacctgtttg aggatcatgc catattgaga aaaacttgcc
    19621 agagaatact caaacaatcc ttgattatct gttttgaaat ggatttctcc atttggacga
    19681 agaatctgtt catctacagc aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt
    19741 ttttttggcc atggatcgga aaaattcaga tagatttgat ctacttcgct gtcagcgaaa
    19801 tactctgtca acgctgatcc atccacatgt agaagtttca ggttaggaag cggttgttcg
    19861 atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca tttctactcc aatataattg
    19921 atttcaggat gggcttttgc cattccagtg atgaactggc ctttccccat accaatctca
    19981 atgtggattg gatgatcgtt ttcaaacaat tcattccatt ttcccttcca caatgttgga
    20041 tcactaatta caaaattcgg gtgagccgct aacatttccg ctgctcccgg acgatttcgg
    20101 acacgcatta tttatctcca ttcctttcct ctaattaaaa aggtctggcc aaatctccgc
    20161 agagtttggg ccaaacctca gccactgaaa attcgtttta attgtaaaat ttcttcgttc
    20221 atccgtcgat gatcttcacg tttgcaatat ctggtgattt cttggagcaa attcatggcc
    20281 gcataccaat gaaatttttc aagcatttcg tcagtcgggc gtatgccata actgatcagc
    20341 cactgattcc agctagacag tggcacataa tttcctaaaa tcgttgcaat atccaccgca
    20401 gggtctgcaa acatgattga atcccaatcg actagatata aataattttt acatactaac
    20461 caattacgat ggtttacatc tccgtgtact actgataagt tttccttatc tattgacggg
    20521 atctgctcac gaagaaaacg atggacaatt tgcagaaatt gatttttctg gagttcttcc
    20581 ggtaattctt tttcatattt ttgcagcatc ttctccggcg tcatgacacg accaccgata
    20641 cgggtcagca tggttttaag agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg
    20701 tcgtttcttt gaccaatctc attaggagtc agcaagcgac cttccagcca ctcttgtgct
    20761 gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa gttttggtgt aattccttcc
    20821 cttgaaagag cagccaacat cggagtcgtg tttcgtttaa taaagacttt gtcctcatca
    20881 cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg
    20941 tctaactgaa actccatcat gcattctccc gtcctcttac tctttgtttt gcagtactta
    21001 ttattctagt cgcctgaaca ctacacgtca agcacatata cgttcttttt catttaattg
    21061 ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga acagcattct tactgtcaac
    21121 gctctatttt tcattctcat ttttacccta cacagctttg ttttcagtct gacatcttct
    21181 tcaaacgagt tggtacatat agtttaacaa agagcatagt aacaatcaaa taagccgcta
    21241 gcggaaccca tcgctgctgc ccatctaata cgactgcagc aatcaaacca aaaataaccg
    21301 acgcgataat taatacccat gtgagtagat actgaatcgc ctgcttcttc tgattcttat
    21361 ggattggata aagctgaacg agaaccatat agcggaactg gttcgctaaa ggtaacagtt
    21421 ggaaaccgat caaatataaa aataagaaac cgataatcag actaaaataa aagtcgacta
    21481 ctccggcaat caataaccca cctataatta ctaatcggaa ataaagacca ctaaaatcag
    21541 aacctcttgc caatcttctt gcaaacaagt aaagataagt gttttttgat tctcttttaa
    21601 tatgagtcaa tataggatcc aaatagcgtc ttcttttgat ctttgccgta atttcaggaa
    21661 catctgtgaa taaattgata aattgataga tctgatgcaa cctatgttct tctacatgca
    21721 ccattttctc ccaatccaat cctttttctt gttgcttcca caaaaaccag taatatagac
    21781 ttgcctgtac taatgcacct gctattcctg cccatattag ccaataaaat gatagaaaca
    21841 aaaagatcag agaagaaaaa aaccagccta atttccaata acgaataact tgttgatcta
    21901 gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa tttcaaactc cataatatga
    21961 tcagatagac gaaaaacatc gaaaaggatt gctccgtact aaccaccact aatggcatgg
    22021 ccaatccgca tgccaatatc aaaaagatca acggaaaaat gcaagaatag cgaaaagctc
    22081 gttccaaata ggtttgcatt tctttttctt taggaagtaa aaaaacaata tctgctgcct
    22141 ctgcaaaagt agccatctta cccacgaata aacagagcat ccaaaataca gagattatga
    22201 gccctccaat tgcaaacggt ggctgcaggg ttttcaacca attagaataa tataatccca
    22261 caccgccaat caagaaaaga caaactaaaa caaaatgatc gttcaataca taacgcatat
    22321 atttcatcat tttcttttga tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc
    22381 tgctcctttg ttaaagcaat atagatttcg tccaaagaag aatccggcag ctgaaattct
    22441 tttctcaaca tctcaagaga accgtctgct cgtatctctc cgttatgtaa gaccacaaaa
    22501 cgatcacaat atttttctgc agtagctaaa atatgcgtag acatcaaaat accggctcct
    22561 tcttttttca tttctaccat caattcaagc aatgcattga tagctagtgg atccaagcct
    22621 aaaaatggtt cgtcgatgat atataaagaa ggctcgatca aaaaggcgca taagatcatc
    22681 actttctgtt tcattccctt tgaaaaatta gctgggaacc agtcaagacg attagataac
    22741 cgaaaagtct ccaacaactt ttctgctctt ttcattgcta tatccatagg tatatcgtag
    22801 gccatcgcag tgacttcgat atgttctctc aatgtcaatt cttcatataa agaaggagtc
    22861 tctggaatat acccaatttg tttgcgatat tcttccgtat ttttctttaa ggatagatga
    22921 ttgatttcta tcgttccttt tgaagcttcc agcagaccga taatgtgctt gatggtcgta
    22981 cttttccctg ctccgttcag tccaatcaac cctactatct ctccaggctg tacatcgaaa
    23041 ctgatatctt ttaacacagg gagatgtccg tacccccctg tgacatgatc gataattaaa
    23101 ctcattcttt ttcctccgcc atttattttt atttattata ccacgaagaa aatgacggta
    23161 ttttgttttt ttccttacaa tagctttaac ttatggtaaa gttaagctga agtatttatt
    23221 ctttatagaa aggatgtttt atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga
    23281 aatcccaagc tataaaatat atgaagacga caaggtctat gcttttttag acatctctca
    23341 agcaacgaaa ggacacacat tagttgtgcc taaacgtcat gtagcagata tttttgaata
    23401 tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct aagattgctc aagcattgga
    23461 aaaagctttt ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg aactagccta
    23521 tcaatctgtt tttcattcac atatccattt agtccctcgc tacagcaaag aggatgattt
    23581 ttctattcac tttgtcaatc atcaagatag ttatggatcg gaagaactaa aagctattca
    23641 agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat
    23701 ttggagctgc tgctggcact gtcggaggtc tgttattggc gcccaggagc gggaatgaaa
    23761 caagaaagaa actgatcgaa gaattggaag aagcaacaac tctgacaaat gatttaaata
    23821 acagcctgaa tcattttaaa agcgcattga tcgaaacaaa agaaacagct gaatctgtga
    23881 ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga ttttaaattc caagccgaac
    23941 cacgtatcac acaaatcaat gaacaagtag aaaaactaaa agctgatctg ccagagtatc
    24001 ctgaacaaat ataataatga ttattttctc aacagattta actatataag tgagaaaagt
    24061 ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg tttgaaacta ccacgacaac
    24121 gtttttaaag ga
//
LOCUS       DASJRQ010000041        23830 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJRQ010000041 DASJRQ010000000
VERSION     DASJRQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.041"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..788)
                     /locus_tag="U4A80_001435"
     CDS             complement(54..788)
                     /locus_tag="U4A80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1975063.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="U4A80_001436"
     CDS             1115..1525
                     /locus_tag="U4A80_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1975064.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="U4A80_001437"
     CDS             1518..2219
                     /locus_tag="U4A80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1975065.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="U4A80_001438"
     CDS             2219..3805
                     /locus_tag="U4A80_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975066.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="U4A80_001439"
     CDS             3824..4045
                     /locus_tag="U4A80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1975067.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="U4A80_001440"
     CDS             4042..4803
                     /locus_tag="U4A80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1975068.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="U4A80_001441"
     CDS             5183..5692
                     /locus_tag="U4A80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1975069.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="U4A80_001442"
     CDS             complement(5767..6306)
                     /locus_tag="U4A80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1975070.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4A80_001443"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4A80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1975071.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="U4A80_001444"
     CDS             7319..8170
                     /locus_tag="U4A80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1975072.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="U4A80_001445"
     CDS             8198..8833
                     /locus_tag="U4A80_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1975073.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="U4A80_001446"
     CDS             8853..9626
                     /locus_tag="U4A80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1975074.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="U4A80_001447"
     CDS             10034..10831
                     /locus_tag="U4A80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1975075.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="U4A80_001448"
     CDS             10809..11222
                     /locus_tag="U4A80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1975076.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="U4A80_001449"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="U4A80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1975077.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="U4A80_001450"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="U4A80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1975078.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="U4A80_001451"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="U4A80_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1975079.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4A80_001452"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4A80_001452"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1975080.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="U4A80_001453"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="U4A80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1975081.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="U4A80_001454"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="U4A80_001454"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1975082.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="U4A80_001455"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="U4A80_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1975083.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="U4A80_001456"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="U4A80_001456"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1975084.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="U4A80_001457"
     CDS             21906..22484
                     /locus_tag="U4A80_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1975085.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="U4A80_001458"
     CDS             22536..23018
                     /locus_tag="U4A80_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1975086.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="U4A80_001459"
     CDS             23135..23509
                     /locus_tag="U4A80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975087.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DASJRQ010000042        23388 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJRQ010000042 DASJRQ010000000
VERSION     DASJRQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.042"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..2187)
                     /locus_tag="U4A80_001460"
     CDS             complement(43..2187)
                     /locus_tag="U4A80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1975088.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="U4A80_001461"
     CDS             complement(2544..3917)
                     /locus_tag="U4A80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1975089.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="U4A80_001462"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="U4A80_001462"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1975090.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="U4A80_001463"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="U4A80_001463"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1975091.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="U4A80_001464"
     CDS             complement(5365..6378)
                     /locus_tag="U4A80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1975092.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="U4A80_001465"
     CDS             complement(6459..7058)
                     /locus_tag="U4A80_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1975093.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="U4A80_001466"
     CDS             7244..7945
                     /locus_tag="U4A80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975094.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="U4A80_001467"
     CDS             7955..10666
                     /locus_tag="U4A80_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975095.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="U4A80_001468"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="U4A80_001468"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1975096.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="U4A80_001469"
     CDS             complement(12805..13986)
                     /locus_tag="U4A80_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1975097.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="U4A80_001470"
     CDS             14238..15761
                     /locus_tag="U4A80_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1975098.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="U4A80_001471"
     CDS             complement(15830..16558)
                     /locus_tag="U4A80_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1975099.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="U4A80_001472"
     CDS             complement(16571..17851)
                     /locus_tag="U4A80_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1975100.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="U4A80_001473"
     CDS             complement(17992..18594)
                     /locus_tag="U4A80_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1975101.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="U4A80_001474"
     CDS             18839..19045
                     /locus_tag="U4A80_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975102.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="U4A80_001475"
     CDS             19357..20076
                     /locus_tag="U4A80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1975103.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="U4A80_001476"
     CDS             complement(20157..20840)
                     /locus_tag="U4A80_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1975104.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="U4A80_001477"
     CDS             21058..23244
                     /locus_tag="U4A80_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1975105.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7779 a         4212 c         4589 g         6808 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgt
//
LOCUS       DASJRQ010000043        22734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJRQ010000043 DASJRQ010000000
VERSION     DASJRQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.043"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1957)
                     /locus_tag="U4A80_001478"
     CDS             complement(26..1957)
                     /locus_tag="U4A80_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1975106.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="U4A80_001479"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="U4A80_001479"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1975107.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="U4A80_001480"
     CDS             complement(2886..3638)
                     /locus_tag="U4A80_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1975108.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3798..4007)
                     /locus_tag="U4A80_001481"
     CDS             complement(3798..4007)
                     /locus_tag="U4A80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975109.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4018..5345)
                     /locus_tag="U4A80_001482"
                     /pseudo
     CDS             complement(4018..5345)
                     /locus_tag="U4A80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5481..5636
                     /locus_tag="U4A80_001483"
     CDS             5481..5636
                     /locus_tag="U4A80_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975110.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5710..6297)
                     /locus_tag="U4A80_001484"
     CDS             complement(5710..6297)
                     /locus_tag="U4A80_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1975111.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6380..7630)
                     /gene="clpX"
                     /locus_tag="U4A80_001485"
     CDS             complement(6380..7630)
                     /gene="clpX"
                     /locus_tag="U4A80_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1975112.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFVGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7877..9166)
                     /locus_tag="U4A80_001486"
     CDS             complement(7877..9166)
                     /locus_tag="U4A80_001486"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1975113.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9320..10252)
                     /locus_tag="U4A80_001487"
     CDS             complement(9320..10252)
                     /locus_tag="U4A80_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975114.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10409..11068)
                     /locus_tag="U4A80_001488"
     CDS             complement(10409..11068)
                     /locus_tag="U4A80_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1975115.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11071..12096)
                     /gene="sppA"
                     /locus_tag="U4A80_001489"
     CDS             complement(11071..12096)
                     /gene="sppA"
                     /locus_tag="U4A80_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1975116.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12109..12399)
                     /locus_tag="U4A80_001490"
     CDS             complement(12109..12399)
                     /locus_tag="U4A80_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975117.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12808..14613
                     /gene="glmS"
                     /locus_tag="U4A80_001491"
     CDS             12808..14613
                     /gene="glmS"
                     /locus_tag="U4A80_001491"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1975118.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14685..16040)
                     /locus_tag="U4A80_001492"
     CDS             complement(14685..16040)
                     /locus_tag="U4A80_001492"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1975119.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16064..17233)
                     /locus_tag="U4A80_001493"
     CDS             complement(16064..17233)
                     /locus_tag="U4A80_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975120.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17230..18117)
                     /locus_tag="U4A80_001494"
     CDS             complement(17230..18117)
                     /locus_tag="U4A80_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1975121.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18306..21998)
                     /gene="nifJ"
                     /locus_tag="U4A80_001495"
     CDS             complement(18306..21998)
                     /gene="nifJ"
                     /locus_tag="U4A80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1975122.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22197..22718
                     /locus_tag="U4A80_001496"
     CDS             22197..22718
                     /locus_tag="U4A80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1975123.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6331 a         4940 c         4044 g         7419 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
     3781 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
     3841 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
     3901 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
     3961 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
     4021 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
     4081 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
     4141 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
     4201 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
     4261 tacttctaga tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac
     4321 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
     4381 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
     4441 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt gcatttgtgc
     4501 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
     4561 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
     4621 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
     4681 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
     4741 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
     4801 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
     4861 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
     4921 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
     4981 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
     5041 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
     5101 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
     5161 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
     5221 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
     5281 tttttttctt cttcatttaa gtctgtgagc tggatagtca tcaataattt atcttcgcca
     5341 atcattttct cacctctttc aaatcttttt ataatttaat tataaccaat gataaagaaa
     5401 aactaaaact ttatgtctta gaaaccaatt tggacgatta catgatagac taagtaaaaa
     5461 gaaacaaaga ggtgtttaca atggggttga aatttgaacg tacagataat ttagataaac
     5521 ttttcgaatc atttgctgtc gatccggaaa aaacagttgc accaaaagaa gagatcgatc
     5581 gatttctaaa accacagcca caaaatgaaa agaaaaaaga caagaaacaa aagtaagtta
     5641 agctcaaaaa aagacttacc agccaaactt ttatgaaaga gtttgactgg taagtctttt
     5701 ttattctttt tatgattcaa ttgcttgttc gattgctgcc cacgcttctt cttttccctg
     5761 ttttgtaacg gaagaaaaga gaataaacgt gtcattgcga tcaaaatcca acgctttttt
     5821 gatttcgctc tcatgcttgt tccatttacc acgagggatc ttatctgcct tagtggcaac
     5881 aacaattaca gggatatcat aatacttcaa aaattgatac atctggatat cttcttgaga
     5941 aggcttatga cgcaaatcta ctaaagaaac gaccgcacgt agctggtctc tagtcgttaa
     6001 atatgtctcg atcatctttc cccattttgc tcgctcagtt ttggatactt ttgcataccc
     6061 atatcccgga acatcgacaa aatgtaatgc gttctcgatc aaataaaaat tcaatgtctg
     6121 ggtctttcct ggcttaccgg aagtacgggc taaattcttt cgatcaatca atgtgttgat
     6181 aaaagaagat ttccctacat ttgatcgtcc tgccagagct atttctggca attgtgtatc
     6241 tggatattgt tttggtgcca ctgcacttat gataatttca gcttgatgga ctttcatggt
     6301 ctctctcctt aacaaggtga tgaataaagc aagcggctgt cgcaaaagcc aaaatgactt
     6361 ttacggcagc ctcttttcat caacccgctt ttttctctga atctttatag attactgttg
     6421 gctcaccaga caattcagct gcttcttttg tgataatcac tttttcgatg ttttcatttg
     6481 aagggatatc aaacatcaca tccatcatga tttcttcaat gatggaacga agtcctcgag
     6541 caccggtatt gcgttcgatt gcttttttcg caatggctct caacgcttca ggttcgaatt
     6601 caagttcggt attatctaga gaaagaagtt tttgatattg tttgactagc gcatttttcg
     6661 gctcagtcaa gatccgtacc agatcatctg ttgtcaattt ctctaaagct gccataacag
     6721 gtaatcgtcc gacaaactca gggatcaatc cgaattttag taaatcttca ggaatgatat
     6781 gctgcatgac actttcgtct tcagaaagtt tttggttttg tgtaccaaat ccaatcgtct
     6841 tttcgcccat acgattcttc acaatcgttt cgatgccatc aaatgcacca cctacgatga
     6901 acaatatgtt cgtcgtatca atctgaataa attcttgatg tggatgttta cgtccgccct
     6961 gaggtggcac acttgcaaca gttccttcta gaattttcag caaggcttgt tggacacctt
     7021 cgccggaaac gtcacgagta atagatacat tttcgctttt acgcgcaatt ttatcgatct
     7081 catcaatata gatgatccct ttttctgcaa gttccacatt gtaatcagca gattgaagca
     7141 atttaagcaa aatgttttct acatcttccc ccacataacc agcttctgtc aaacttgtcg
     7201 catctgcgat tgcaaaaggt acattcaatg tttttgctaa tgtttgtgct aaaaatgttt
     7261 taccagaccc tgtaggtccg atcaagcaga tattgctttt ttgcagttca acatcatctg
     7321 tgttttcttg tgcttgctga ttgacacgtt tatagtgatt gtatacggca acagctaacg
     7381 agcgttttgc gcgatcttgt ccaatcacgt agttattcaa tacttcaaga atttctagtg
     7441 gtttagggac ttcagtgaat tcgcgtacag cttcttcata aaattcttca tcaatgattt
     7501 ctttacatag gtcgatacat tcattacaga tgtagacccc tggaccagct acgatctttt
     7561 tcacttcttc ttgggttttc ccgcaaaaag aacagcgtac ggtctcattg ctacttggat
     7621 tgtcatacat ggtcttcacc ccttaacttg caaaaagtat gcaagaaact atcaaagact
     7681 ttacagtctt ttactatcat aacatagttt agtaaaaaga aaaagtgttt aacaaaggat
     7741 caactgacct atacctttgc attttttact cttaacaaaa aataaggaac tgtgacaaaa
     7801 tcgtgtcaca gtcccttatt tcgaataaac aattttctag aagtagcttc ttcagaaatg
     7861 atttattact tgatttctat tctttcgctt ctgtcgctgt acctgtgatc aattcgattg
     7921 cttttttcat tgtcacatca tgttccagca tatcttctgt caacacgcgt ttgatttgtt
     7981 cgattggcat gttgtatgtt tctgataatt cttggatttc tgcattgatt tcttcttctg
     8041 ttgcttgaag attttcagca gctgcaattg cttcgatcac aagatttgtt cttgtgcgca
     8101 tttctgcttc accttcgaat tgtttatgca agtcttcttc tgtagaacca gttaattgat
     8161 agtacatttc aggagagata ccttgacgtt gcatgttgtt caagaattcg tccattgaac
     8221 gatgtacttc atcatgaacc atcacgtgtg gcaattcaac gatttctgcg ttttcaacag
     8281 ccatacggat cgctgcttca tcttttgcat cgtccgctgc tttttctttt gtttctacta
     8341 attctttgcg atatttttct ttcaattcgt ctaatgttga tacttcgtca tcaacatctt
     8401 ttgcgaattc gtcatctaat tcaggcactt cttttgcttt tacttcatgg attgtcactt
     8461 tgaagactgc ttcttttcca gcaagatctt ctgcttgata atcttctggg aaagttacat
     8521 ttacatctag actttcacct gatttatgac caactaattg atcttcaaaa cctggaatga
     8581 atgagcctga acctaattca agtgagtaat tttcgccttt tccgccatca aacgcttcgc
     8641 catctacaaa gccttcgaag tcgattacta ctgtatcgcc attttcagct gcagcatctt
     8701 ctttgataac taattcagct tgtgcttctt gttcacgttt gatacgtgca tccacgtcag
     8761 catctgttac ttcacggtct tgtttttcaa caactagatc tttatattgt cctaatttta
     8821 cttctggttt cactgttact tcagcagtga ttacccagtc ttgacctttt tccatgcttt
     8881 caacatcgat ttttggttga gaaacaggat cgatgccagc ttctttgaca gcagcttcat
     8941 aagcttctgg taatacagca ttcaatgcat cttcatataa agcttcttca ccgtacatac
     9001 ggttgaatac ttgacgagaa actttccctt tacggaatcc aggtacgttc aagctttttt
     9061 tcactttatt aaacgcagtt gttaatcctt tttggatttc gtcttgcgca atcgtaaatt
     9121 ttaaaacacc atcattcgtg cctttttttt caaatgttgc agtcatttaa ttccctccga
     9181 gtttagaaat tcattttata catcaaaaac gtttcatgca tacctttaaa tagtacacta
     9241 accctttcaa attgtaaaca tattgaatgg atagaatcct tgtttttcat aacattttta
     9301 tctttttcac tgataaaatc taatggaaga agatcatttg ctgcaaaatt ttttctcttt
     9361 tttttaaaaa atcttgtttt tcatcttctg taccttcata catcgttttt cctgtacacg
     9421 cctctacagt cagttgaaac cagcgcttcg ggtcttcgat gtatgtatca tagacaggat
     9481 aaacaattgc agattgcaac gtgaattctt cttttaacac ggaactaagc agcgcatctt
     9541 cattcccgta acgatctgaa aatacacata attgctggta acttgcctgc tgctgcggct
     9601 ttggcagatc ttttggtatg actttgacta acttttcttc aatcgtcaaa tagaaaacag
     9661 gatggcattc gttcaattgc ctaagtgtct ccagtaattt tgcccttgct aaaatatgga
     9721 cctctggcga tacaataagt ttactcgctg tttggataaa ctcatgataa ggaagctgtt
     9781 cgatctcatc aatagacgca agctgacgtg caggttccaa cgaaggaagt tcctccgatt
     9841 tattgtggag tgccttgatc ttctgcctct ccatttgtcc ggataattct tctgcaaatt
     9901 ggattttttc aatgacccgc tgctcgaaag cttcataaaa atctgtactt gctatcaatt
     9961 ttctagcata taaaaaatct tgtgtactga ttaacaagtc aaaataaaat tcagcaaact
    10021 cctcattttg aagatactgt ttttcatgca agagtgcttg cttcaatgct tcttttttct
    10081 cgtccaactc gtaaagacaa aaaacaagca ggctattagc ctcaaagctt tctttcaact
    10141 cgtaagctcg ttgaaataat tcttttgcct gctgatattg ctggtttgct agtgcttgtt
    10201 gtgccactcg cattaaattc tcatattttt ctggaaaatc aatcggcttc acgcagtgtc
    10261 ctcctcttgc tcatattcag ctcatgttca atagtagcaa aacaaaaaaa gcatgacaat
    10321 taaaaataac tgaagcaccc tctaaaagct agattttaga tctgactttt agaaggtgct
    10381 ttcaactgtt actgcttttt attgctcatc agctgttgac ttcctggttg aacgctttga
    10441 tcagattgat cgtaacaaca cttgtattag tgaaaaaatc gcccagatgt tgctttttct
    10501 gattaaagac tgctggaaga taccctaaca tcagcaaagg aaattttaga atgaaccggc
    10561 aagcaccttc tctaactaaa acggttgacc agctaagttc ttcttcatca aagctgatga
    10621 ctcgaatacc aaagatcatt ttcccaatcg tttggccatg attgaatttc gtcaataaaa
    10681 taaaatacgc tagataaata agtaaggctg tgaatccgta gagactcaga taagaatctg
    10741 aaaaattaag ccaacctaga tttttggcaa taccaagtgt taaacgcgta atagctccga
    10801 tacacaaaag atcgaccaag aaggcaaaca ttcggataaa aaaacctgca tagaaatact
    10861 taggaaaatc atttgctttt ggcggttcat ttcttgtgtc cgtatacttt ttccatttac
    10921 gttgtttctt tttgatttct tcgggcgtca aaggctctct ttcaaaagag ctcagtacct
    10981 tttgggaaaa gttggagtca ggtcctacag caggctgctg ttgcttatct ttttcttgtt
    11041 ccttattttc atttgaaagc tctgacatgc ttactcacct ccgtaataat acattgcttt
    11101 tggtgattcc gctgtcccta gattctcaag aacggataag atctgactag tctcagaagg
    11161 cttcaatccc tggaattctg ctagtttact tcctaaccat gtactagcaa acccagatgg
    11221 cgtggaagaa tattctacta gttccgcatc ttcaagctgt tgttcctttc gtatagcttt
    11281 taaagcatct tcaggaaatc ccaattcatc gactaaacct acttcttttg cttggacacc
    11341 atcataaatc cttccatcag caattttttt gacttcttct tcagatttat tccgtccctc
    11401 gctgacgatt gctacaaaac gactatatgc gctgtcgata tatgcttgaa gcaccgcttc
    11461 ttcttctttt gtctctggtc gcgttcctga acccatatct ttcaacgctc cgcttttaac
    11521 tgttgtatct tccacaccca gtttttctag aagtcctgaa taattcaatc ctgacatgat
    11581 cacaccaatt gagccggtca ctgtttcttc tgttgcaaag attttatctg cttgagcaga
    11641 aatatagtag ccaccgctag ctgccatgtt tttcatgctg acatacattg ggatatcatg
    11701 ttctttacga atcgtatcca atttcttagc gatttccgca ctttcgtaga caccgccacc
    11761 aggagaattt acttctaaaa agataccttt gactgttgga tcttcttcaa tcgccttcag
    11821 ttgttccata aaatttttat gattgtaacc ttcagaagca aataaacctg aagaccctcc
    11881 cgcagcaatc gttccatcta ctgataattt tacgattttt tgcgtactgt ctccctcttc
    11941 caacacattt ggagctagtt cttcatcccc atataaccaa ctgttcaatc cctcaatttg
    12001 atcgttttcc tgaggcttag tcaaactagc tgaaactaac gaaaccacaa ataatcctgc
    12061 agcaatcaga actgctgccc aacgtctttt attcattctt tctcctttct aatacaagtt
    12121 ttttatgacg atcacagttt ctgactgctc agaagtgtcg aaccgctctg ctttttcttc
    12181 ccgcgcatac ccggaaaacg actcttttgt taattcatct tctaccgtaa actcacgagc
    12241 ttgagcaact tccaaggcat caggtgcgat ttcttggatg ctatcccagc cagtatctaa
    12301 aaatattttc gtatcatctg atacaccagc tgatgcttcg aatttggaca attgtttttt
    12361 taacattccc agtgtcatga tctcaatttt tgaatccatt tttactcctc ctctatccga
    12421 ttactttcta ttgtaccgaa ctcctgaaaa agatgataga taaaaaccca ttattttttg
    12481 acataaaaag gacatttgac aaagaatttg atatatacca atttattttt tattcttttt
    12541 ttgttgacaa gaacattcgc aaaaggttat gattttaaat gtttccagat aaaaaacaaa
    12601 aaggaggata gttatacctt ttaggatagc gccaggtctg tgtaaacaga tgacgaggag
    12661 agggtttatc gaaagattcg gcggatggcc ctagggactg cactctacag aaaatccaca
    12721 aaaaatgtct gcaaagataa aacaaaagga ttttcctgca gctggaagca ttcatacgaa
    12781 ttcattatag aaagtaggat gttttttatg tgcggaattg ttggaatgat tggattaaaa
    12841 aatgtaacac caggattgat tgacggtcta gaaaaattag aataccgtgg ttatgattca
    12901 gctggtatct ttgtctcaga tggcacaact gattatctgg tcaaagcaca aggcagaatt
    12961 caaaatttga aaaacaaaat aaatattgat actacaggaa atataggtat tggtcataca
    13021 cgatgggcaa ctcacgggca accttctgaa gaaaacgccc atccacatac ttcccaaagt
    13081 ggacgcttcg tgctcgttca caacggcgta atcgaaaact ttgaagaact taaaattgct
    13141 tatttagcaa atgaccactt tattggtgag accgatactg agattatcgc tcatctaatt
    13201 gaaactttcg cgaaggatac tacaacgcaa gaggcgtttt taaaagcttt gcgggtcatc
    13261 aaaggttctt acgctttcgc tttgattgat cggacagcac ctgatgttat ttacgtcgct
    13321 aaaaataaaa gtccactatt aatcggactc ggcgatggat tcaatgtgat tgccagcgat
    13381 gcaatggcta tgcttgctca tacgaaggaa ttcgttgaga ttgaagatga agagatggtt
    13441 accgtcacat cagaaaaagt tattattcaa aattttgcag gtgacatcgt agaacgttct
    13501 tcattcgaag cacaagtaga tgcttcagat atcgagaaag gtacctatcc tttttatatg
    13561 ctgaaagaaa tcgacgaaca gcctattatc atgcgtcgta tcgctcaaaa atacgtaaca
    13621 gaagacaatc gtgtctctct tgatcaaaag ctggtcgata cattgtctga tagcgatcga
    13681 atctatatcg ttgcttgcgg aactagttac catgcggggt tagcaggtaa acagactttg
    13741 gaaaaattga cacagattcc tgttgaggtt catttagcca gtgaatttgg ctataacaca
    13801 cctcttcttt caaaaaaacc attctttatt ttcttgagtc aaagtggcga aacagcagac
    13861 agccgccaag tgctggtaaa aatcaaccgt ctcggctacc cttcacttac gattacaaat
    13921 gttgctggtt ctactctttc gcgggaagca tcctttacac tgctattgca tgctggaccc
    13981 gaaatcgcag tagcatcaac gaaagcttat actgcacaaa tagctgtact aactttatta
    14041 gccaaagcta tcggtgataa aaaagaattt gaaacttctc ttgtattcga tatcactcat
    14101 gaattgagtt tagttgctaa tgcgatggaa tcggtcatcg cccaaaaaga ttatttagaa
    14161 gagttggcag aagtatatct cagcgataca agaaatgcct tttacattgg acgtggtgct
    14221 gactacaacg tttctcttga agctgctttg aaattgaaag aaatcagtta tatccaggca
    14281 gaaggttttg ccgctggcga attgaaacat ggaacgattg ctttgatcga agaaggaaca
    14341 ccagtgatcg gaatcatcag tgaagaagtg actggtgcgc atactcgagg aaacctgaaa
    14401 gaagtcgaaa gccgtggcgc taaaacacta gttattgtct cagagggatt agaaaaggaa
    14461 agtgaccagc tggtgctacc aactgtccac ccttatctaa ctactctaac cacagtcgta
    14521 ccgactcaat tacttgcata ttacgcaacg ctattacggg gattcgacgt tgacaaacca
    14581 cgtaatttag cgaaatcagt gacagtagaa taataaaaga acaaggacca gtgtcagaca
    14641 tctgacactg gtccttgttc ttttattttt tatgaaaata tctcttaatc cagtccgatt
    14701 tcttctttga ctactgccgc gattttatcg acatagtaat taactttctc atcagtaggc
    14761 gcttctgcca tcactcgaag taatggttct gtaccggaag gacgaactag gattcttcct
    14821 tctgatccca tttcagcttc tgcctcttca atcacttttt tgattgccgg aacatccatc
    14881 gcaccgtttt tatcacttac gcggatattc actaattttt gcggatagat tgttacctct
    14941 gctgcaagct ctgataattt cttacctgtc tgtttcatga tattcagtaa ctgaattcct
    15001 gaaagcatcc catctccagt tgtgttaaaa tctaagaaaa tcatgtgccc agactgctcg
    15061 ccgccaaagt tataatcgtt tttgcgcatt tcttctacaa cgtaacgatc accaacttgt
    15121 gtaacgacat ctttcaaacc gattccttcc accgctttgt ggaatcctaa gttactcatg
    15181 acagtcgtaa cgatcgtatc ttttttcaat cgattttttt ctgccaagta tttagcacag
    15241 ataaacatga ttttatcacc gtcgacaatg tttccaagtt catccaccgc aataatacga
    15301 tcgccatcgc catcgaaagc tagtccagca tctgctcctt tttcaacgac catctcagct
    15361 aaacgttctg gatgggtaga gcctacgccg tcattgatat ttaacccgtc tggatttgtg
    15421 cccattgtat aaaaatctgt ttcaagatcg gcaaacaaac gattaacact tgttgaagtt
    15481 gctccgtttg ctgcatcgac acaaatggtc aaaccggaaa ggtcaccgtt gatcgtttgt
    15541 acaaggaatt gagaatattt caataagcct tctggaaatt cttctacagt tcccaatcct
    15601 tcagctgatg gacgaggcaa ttcatccact tcagcatcta ataatgcttc aatttctagc
    15661 tcttggtcat ctactagttt aaaaccatct gcaccgaaaa atttgattcc attatcttgt
    15721 gctggattat gagaagcaga aatcatgacc cctgcgcttg ctttttgcaa acgtgtcaga
    15781 taagcaacac ctggtgtaga gatcacacct agctggaaca cttcgatacc caccgataaa
    15841 agtcctgaaa tcagcgcttc ttctaacatt tgacctgaga ttcttgtatc ccgccctact
    15901 aacacgcgtg ggcgttttcc ttcttcttca tgctggctta ggacataacc tccgcaccgt
    15961 cccagcttaa atgcaagttc tggcgtaagt tccttgttgg cttcaccgcg tacgccgtct
    16021 gttccaaaat atttacccat agttgtaaaa ttactccttt aacttaattt tctgaggaat
    16081 ttgttctgct catttgtgtt gattcgcttg tttctgatgt ttcttctgta cttgtggtcg
    16141 agctgcttgt cccagattcc gaactggaag aaatggttgt tgtcgatggt tccgttcctg
    16201 aatcagcaga tgaaatactt ctactcgtac ttgtattttc ttgtgtccga ctgctcgtcg
    16261 aagcagagct actgcttgac gtttgcaaca cgggtgtaat ctccacacta actgattcgg
    16321 gcttgatact taacccctct tctactggta tttcaatttt acgagttgtt cgatgattga
    16381 tacctgagac attgatcggt acagcaatac tgtcaccgat cgatgcaagc aaatcgctag
    16441 agccgcttac ttctgcttcc agttttgaaa aactgaaatc atagctttct accccggcag
    16501 ctggtgttcc ttgctgtatc ccataaagtc gtaccgactt gcttggcttg atcgaatcag
    16561 ctctcactgt tacttgcact ggatcagaga cgatacttaa tacttcccct gctgcattca
    16621 gcgcctgtat tgggacctca ctattaatcc catcatctgt gaccatcgaa gcgtccacat
    16681 cagctactac tcgtttgatt tctgccaatg ttttgtcacc tgtagtgata gagacttttt
    16741 taggactaac ggaagtatca tcaagttcat atccagatgg tgtgaacgtt gaaggaatca
    16801 acgtttccac atcgaaatct tttattacct ttttctcaac cgttactgta atagtcgatg
    16861 gttcgatctt tgctgtgaca gcactgctca aattttgaat ttttagcgga acttcatgtg
    16921 ttccttcgcc cagcttagtg atatctgctg tgacgcggaa catccgtgta tcttcgtttg
    16981 cctctgcatt caactgaact cggtttgcac tggaaagttt gacagtcact gcattttcat
    17041 atccatggat atagtagcgc tttgaatcat aagaaatctt gatcggcacc cccgtaactg
    17101 tttgattata cgcttcattt ccagaaagcg tatttggaac ggactggcca tttgcattga
    17161 agtacagcgc aatactgaac agcaatgcaa taaaactgta aagaatgctt gtccgtttct
    17221 cttttgagat catttgcgat cccctccctt agtcataccg tctaggaaag attgaatcac
    17281 gtttttcttg ttttttgttt tctcttcagc aattagttct ctacgcaaga ttgccaaata
    17341 ttcttgctgg gataagccag ccatcagttc attatctaac gtgatgctga cgtccccggt
    17401 ttcttcagag acaacgatgg taatcgcgtc actgacttcg cttataccga cagctgcccg
    17461 gtgtcgagtc ccgaattctt tagggatcaa catactttct gatagaggaa gataggcgct
    17521 tgctactgcg atctttcctt cttttacaat aactgcaccg tcatgcagcg gtgtattagg
    17581 aataaagata ttgatcagca gctccccagt gatatctgca tctaaagcaa tccctgtctc
    17641 aatatactca tctaatccag tatgacgttc gatagtgatc aacgcaccga tttttcgctt
    17701 ggacatatat tggatggctt tgtcaaaaga caagatcatc ttctcatctt cctgctgttc
    17761 gctttttgat tgtttgaaaa gcgaactgcg tccaagatgt tccagtcctc tccggatttc
    17821 cggctgaaaa atcacgatag cagctatcac cccgtacgta atgacttggt tcatcagcca
    17881 agacaaggtg tgcagaccaa tgatttccgc tacgattcgg ataacaataa aaatagctac
    17941 acctttcaga agctgaatcg cttttgtgcc tcgcagcagt tggatcagtt tgtatacaag
    18001 ataccagaca accagaatat caagtatgtt caccaaaaaa ttcagagaga acaagtcacc
    18061 ggaaaacatt tgctgccaat agtccaaatt aaaaagttcc ccgatttgaa atgacataaa
    18121 aagcctctct tctcttttga gatttcctct ctagtatatc atatgtttta attttagagg
    18181 ctgatatgct cgtatttttc ttgagaaaaa cgcataattt tcaagaaaat tgcaagtatg
    18241 cgttttattt ttctctgcaa taaatatttt taaacttcag agaattattc attatatttt
    18301 ttctgttatt tttccttttt ttcttttgta gcttgttcca tttttttacg tcgttctgct
    18361 ctggcagctc ttctagcagc tcttctttct tctgcttcaa ggtccgtcgt tttttctttt
    18421 ctagtcttaa cgggtacatc gatagattcc tctggttcaa gtcgtttctt gattttttct
    18481 aattctcccg tcagtgcagc atagttgtaa aaacgtgttt tagcgtcgtg gacagtcttt
    18541 tcaaataaag cttttgcaag atttggattt gcgttatcta gcgaggaaaa gcgaacttgt
    18601 ttcctcatga aatcaggcat agcagagaaa tcaggttttt tgtagtccaa agtcattggg
    18661 ttcttgtttt tttcgaccat tgtagggtta tatcgatata atgaccaata accagaagaa
    18721 acagcttctt ttgcttcttt aagcgattct ttcattccgc caagcaagcc atgtgtgata
    18781 caaggcgtat aggcaataat cagagaagga ccaggatact tttctgcttc ttcaaatgcc
    18841 ttgatcgttt gcatttgatt ggcacctgaa gcaatttgcg ccacataaac atttccatat
    18901 gtcatcgcca tcattcctag atctttcttt gaagcatact ttccactagc cgaaaattta
    18961 gcgattgccg aagctggtgt tgcttttgag gtttgaccgc cagtattgga atagacttcg
    19021 ttatccaaaa caaggatatt cacgtctgct ccgctggcaa gtacatgatc gatcccgcca
    19081 aaaccaatgt cgtaagccca tccgtctcca ccaatcatcc attggctgtt ttttacgaat
    19141 agatcctttc tcttgtatat atcctccagt tcagggaaaa ttgatcgttc gctttcgatc
    19201 gcagcttgca gttttgctgc tctttgctgc gttccttcac tctcaaacat gtgtactaac
    19261 cagtcttcca tcaattctcg tagttcttct gatacttttg gcaatacttc tttgattgaa
    19321 cttgccaaag cctcgcgacg agtctgtgcc gctagaagca taccataacc aaattcagca
    19381 ttatcttcca ataaagaatt tgaccatgca ggcccttgac cacattcatt ggtcgtataa
    19441 ggagaagcag gagaagcacc gccccaaata gaagaacagc ctgtcgcatt cgcgatcatc
    19501 attcgatcac cgaacatttg tgtcaataat tttacataag gtgtttcgcc acagcctgag
    19561 caagcgcccg agaattcaag aagcggttga ttgaattgtg tagacaagac agtattcttt
    19621 cctttgactg gatttgcttt ttgtcttaat gtcattgcaa aagcccaatt cacagcttgt
    19681 tctttctgtt cttcataagg cttcatcact aaagcttttt cttttgcggg acaagcctct
    19741 acacacagac cacaacctgt acagtcttcc actgaaactt ggatacggta ttttacgccg
    19801 tctgcgcctc gcatttctct cacgataaat ccttctggcg cttcttccat ttcctcttca
    19861 tctgctaaaa atggacggat ggcagcatga ggacaaacaa aggcacattc attacacatc
    19921 gtacagcgat cactaatcca ctcaggtact tctaaagcga cgccccgctt ttccatagaa
    19981 gccgttccta aaggcatttc gccagttgtc attccatttg ctacaagtgt accaacagag
    20041 agttgatttc cttcctgtgc attcactggt tcaagtattt cttgtatata accagtgttc
    20101 cttttattga ttttttcagg catctccaac gttctccaat ggtctgggat cgcgacttga
    20161 tgaagcagtt cggtcgtccg atcgattgct tgtatgtttt tttctacgat agccattgat
    20221 ttatgtccat agcttttcag ggcttcttct tttagcagcg gaagcacctc atcaaaagga
    20281 atgatgtcag ctaatttaaa aaaagccgtt tccattgccg tattgatccg gcgtcccaaa
    20341 ccgacttctt gggccaatct catggcattg atcgtataga atctaatgtt attttcagcc
    20401 aagtaacgtt ttaattttaa gggcagatgc gtttctaatt gttcgtcgct ccaaagagta
    20461 ttcaataaaa aaatgccacc tggcttcaat cctttgacca agtcatagct atgcaggtaa
    20521 gctggtgtgt gacaggcaac aaggtccgca tgctcgacaa gatacgctga acgtatcggc
    20581 gtatctccaa aacgaagatg agagacagtc agaccgccag attttttcga atcataataa
    20641 aaatagcctt gcgcatattt atcggtgtga tctccaataa tcttgatagc tgatttattt
    20701 gctccaactg ttccgtcaga tccaaatccc caaaacttgg cctggaatgt ttgcggttct
    20761 gtcaaatcca gactttcctt tggttccaaa gattgataag tcacatcatc cacgatccct
    20821 atggtaaacc gttttttgcg tatgcttggg tcttttttca attcatcaaa aactgctgag
    20881 atttggtcgg gagtcacatc tttagacccc aatccgtagc gaccaccaat aacagccggg
    20941 cgaagttcag aatcatataa cgcactttga acgtctaata agagaggttc tccacctgcc
    21001 cctggttctt tacttcgatc aagaacagca attgttttta ctgtatgagg caacttttgc
    21061 acgaatgttt ccactggaaa aggacgataa agatggatat tcaaaaaacc tgttttccgt
    21121 ccgtttgcat tcaagtaatc aatcgtttgt tcaatcgtct gtgcaactga tcccatagaa
    21181 acaatgactt cttccgcatc gtctgctccg taataagtca ccaagtcata agaagttcct
    21241 cgtaatcggt tgatctcttt catatatttt tgaacgattt caggtaattt atcataatag
    21301 gggttgatcg tttcccgttg ttgaaaatgg atgtctggat tctggttcgt accactaaca
    21361 gtcggatgat tgggattcat ccctcgttta cgaaatgaag ctaatttctc ctgattgatc
    21421 agaggagcta attcttcata ttccaaaact tcgatttttt gaatctcgtg actcgtacgg
    21481 aatccatcaa agaaattcaa aaaagggact tctccttcaa tagaagcaag atgggcaaca
    21541 ggtgctaaat ccatcacttc ttgtacactg ctttcagcta acatagcaaa tcctgtttgt
    21601 cttgcagcca tcacgtctcc gtgatctccg aaaatattca acgcatttgt cgtcactgcc
    21661 cgactagcaa cgtggaaaac acttggcagt agttccccag caatcttgta catattcggt
    21721 atcatcaaaa gcagcccttg tgaagcggta tatgtagttg tcagtgcacc agttttcaat
    21781 gatccatgga ccacaccagc tgctcctgct tcggattgca tttctactac ttttactggt
    21841 tgaccgaata cgttttcttt tcctgctgct gaccattgat ctactaattc agccatggtc
    21901 gaactgggtg ttattggata tattgcagca atctcggtaa atgcataaga aatatatgcg
    21961 gctgccgtat tgccatccat tgttttcatt tttcgcatga ttcatcatcc ttgttcatat
    22021 tctctcttat tatctgttcc attataacgt aaaacatgcc atttttttct tttatttata
    22081 aaaatatttt acatgtatct atttgaattg gtcatctaat ttttagacaa acagtctaaa
    22141 tcgttgcctt tttttgtcag acatgtataa actataagcg aaagaggtgt tttaacatgg
    22201 atatatcagt gattgatgca acgaaaacaa atacacaaaa aggaatcctt tatggttcat
    22261 cgaatgctcc taaaaaaatg gtcgaattca tcaacttagc ttgtccgtat tgccgacaat
    22321 ggtttgaaga atcttatgag ctattagaag aagcagtcca aagcgggcaa ttgcagcgag
    22381 tgatcaaatt gttcgataaa gaaaaagaaa gcttacagcg agggaacgtg atgcaccggt
    22441 atctcacaat cagcgatggc caaaaagcga tcaaagagat caaacaaata ttcgatactc
    22501 aagacgagtg gaaacatctt tctctccaag aagtagcgga ttttgcagta gataaactaa
    22561 agctgacaga acaaaaagac gaacagctga gccaagctgt catcaacgaa gctgagcaag
    22621 cacatattcg atttgttcca actgtcatct tgggtaaaga aatctttgat gaatctattt
    22681 caatagagga attaaaagaa ttgatccaaa caaaataaaa aaataaggag cgtg
//
LOCUS       DASJRQ010000044        22406 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJRQ010000044 DASJRQ010000000
VERSION     DASJRQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.044"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U4A80_001497"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="U4A80_001497"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1975124.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U4A80_001498"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="U4A80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1975125.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U4A80_001499"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="U4A80_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1975126.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U4A80_001500"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="U4A80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1975127.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U4A80_001501"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="U4A80_001501"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1975128.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U4A80_001502"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="U4A80_001502"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1975129.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="U4A80_001503"
     CDS             complement(8557..9027)
                     /locus_tag="U4A80_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1975130.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="U4A80_001504"
     CDS             complement(9073..9498)
                     /locus_tag="U4A80_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1975131.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="U4A80_001505"
     CDS             complement(9565..9834)
                     /locus_tag="U4A80_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1975132.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="U4A80_001506"
     CDS             complement(9845..11440)
                     /locus_tag="U4A80_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1975133.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U4A80_001507"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="U4A80_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1975134.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="U4A80_001508"
     CDS             complement(14993..15553)
                     /locus_tag="U4A80_001508"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1975135.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="U4A80_001509"
     CDS             15906..16880
                     /locus_tag="U4A80_001509"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1975136.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="U4A80_001510"
     CDS             17050..18450
                     /locus_tag="U4A80_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1975137.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="U4A80_001511"
     CDS             complement(18528..19250)
                     /locus_tag="U4A80_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1975138.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="U4A80_001512"
     CDS             complement(19329..19835)
                     /locus_tag="U4A80_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1975139.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="U4A80_001513"
     CDS             19958..21757
                     /locus_tag="U4A80_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1975140.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..>22406)
                     /locus_tag="U4A80_001514"
     CDS             complement(21825..>22406)
                     /locus_tag="U4A80_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1975141.1"
                     /translation="TEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDT
                     GNGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPS
                     GFDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAI
                     ALGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
BASE COUNT         6188 a         4883 c         4050 g         7285 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgt
//
LOCUS       DASJRQ010000045        21947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJRQ010000045 DASJRQ010000000
VERSION     DASJRQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.045"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..364
                     /gene="rplT"
                     /locus_tag="U4A80_001515"
     CDS             5..364
                     /gene="rplT"
                     /locus_tag="U4A80_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1975142.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            616..1953
                     /locus_tag="U4A80_001516"
     CDS             616..1953
                     /locus_tag="U4A80_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1975143.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            2001..4130
                     /locus_tag="U4A80_001517"
     CDS             2001..4130
                     /locus_tag="U4A80_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1975144.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            4105..4794
                     /locus_tag="U4A80_001518"
     CDS             4105..4794
                     /locus_tag="U4A80_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975145.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(5202..5815)
                     /locus_tag="U4A80_001519"
                     /pseudo
     CDS             complement(5202..5815)
                     /locus_tag="U4A80_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5951..6145)
                     /locus_tag="U4A80_001520"
     CDS             complement(5951..6145)
                     /locus_tag="U4A80_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975146.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(6195..6635)
                     /locus_tag="U4A80_001521"
     CDS             complement(6195..6635)
                     /locus_tag="U4A80_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1975147.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6639..7484)
                     /locus_tag="U4A80_001522"
     CDS             complement(6639..7484)
                     /locus_tag="U4A80_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1975148.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7633..9051
                     /gene="pepV"
                     /locus_tag="U4A80_001523"
     CDS             7633..9051
                     /gene="pepV"
                     /locus_tag="U4A80_001523"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1975149.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(9108..10055)
                     /locus_tag="U4A80_001524"
     CDS             complement(9108..10055)
                     /locus_tag="U4A80_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1975150.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10278..10628)
                     /locus_tag="U4A80_001525"
     CDS             complement(10278..10628)
                     /locus_tag="U4A80_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1975151.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10828..11907
                     /gene="pepA"
                     /locus_tag="U4A80_001526"
     CDS             10828..11907
                     /gene="pepA"
                     /locus_tag="U4A80_001526"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1975152.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            12051..12371
                     /locus_tag="U4A80_001527"
     CDS             12051..12371
                     /locus_tag="U4A80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1975153.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            12393..12857
                     /locus_tag="U4A80_001528"
     CDS             12393..12857
                     /locus_tag="U4A80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1975154.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            13062..13667
                     /locus_tag="U4A80_001529"
     CDS             13062..13667
                     /locus_tag="U4A80_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1975155.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13706..14995)
                     /locus_tag="U4A80_001530"
     CDS             complement(13706..14995)
                     /locus_tag="U4A80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1975156.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15423..15902
                     /gene="rlmH"
                     /locus_tag="U4A80_001531"
     CDS             15423..15902
                     /gene="rlmH"
                     /locus_tag="U4A80_001531"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1975157.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15987..16496
                     /locus_tag="U4A80_001532"
     CDS             15987..16496
                     /locus_tag="U4A80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEN1975158.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16596..17246
                     /locus_tag="U4A80_001533"
     CDS             16596..17246
                     /locus_tag="U4A80_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975159.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17697..18635
                     /locus_tag="U4A80_001534"
     CDS             17697..18635
                     /locus_tag="U4A80_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1975160.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18648..19700
                     /locus_tag="U4A80_001535"
     CDS             18648..19700
                     /locus_tag="U4A80_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975161.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19966..20601
                     /locus_tag="U4A80_001536"
     CDS             19966..20601
                     /locus_tag="U4A80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1975162.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            20792..21583
                     /gene="msrA"
                     /locus_tag="U4A80_001537"
     CDS             20792..21583
                     /gene="msrA"
                     /locus_tag="U4A80_001537"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1975163.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         6973 a         3854 c         4526 g         6594 t
ORIGIN      
        1 atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa gagtgctaaa
       61 attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa aagaacaagt
      121 aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg acttccgtaa
      181 attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct actcaaaatt
      241 aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag ctgacttggc
      301 tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag ctttggctaa
      361 ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc tttcctcttt
      421 ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc taaaatagca
      481 taattaaggt tttttaattg aaaactgaac atttatggtc accctttaat gaaagcgcta
      541 actacataag ttagaatgag tttgactgat atattagttt gcttatcaga aaaacaaaat
      601 ggacggagga ataacatgag tgatattatt attggtattt tgttagttat ctcattcttt
      661 ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggctttgc catcatggct
      721 actgtatgga tgggattagc gctagtcgga aacacgtttt caccaaaccc ggcgatggaa
      781 gggcaaggag taattgatgt gttgacgcat atctatacga cggggccagc tgagtacgcc
      841 aaatcgatct tggtaaatgt cttctttggt gcctttttcg ggcgtgttct agtagatagc
      901 ggaatcgcag ctaccctgat tcgaaaggta gttgaacttg gaggggacaa gccaagaatc
      961 acgatgagct tactatgtat tgtaaccgct gttatattca tgtcgatgac aggaatcgga
     1021 ccagtgattt ctattgcagt tattgtgttg ccaatcttga tgtcattagg tatttcagta
     1081 ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc taatatcgtc
     1141 aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga aagctatact
     1201 tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt tgtggtacta
     1261 acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc aaacgtacca
     1321 gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc agttttaggg
     1381 gtcgttcttt tagatcttcc aatcatctta ggttttattc tagcaggtat ctgggcattg
     1441 ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt tgcgaaatta
     1501 tttacagatg gcgcagtaga tgttgcacca atggttggtt tcttgatgac cttagcgatg
     1561 ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg agattggata
     1621 ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact aggatttttc
     1681 cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt attagctgtt
     1741 aatccaacga tgtcgccagc ctttttgttc ccgttatttg caatcacaac gattgctcct
     1801 cagcatttag atattacaca atcttgggtt gcttggggat taggttatac aaaagtaact
     1861 tctcgagaat atatgaagaa atccatccca actggttgga tcatcggagc gatcttatgc
     1921 ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata aaaacgatta
     1981 gatgaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat gttggcggta
     2041 cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga aaatcttcag
     2101 tgaaaacaac ccacgatgat gtccgtggag tagccgcagg tgtcgttcag tcttttcaaa
     2161 attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca cattctacca
     2221 cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta atcgggatgg
     2281 caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac gatatcgatt
     2341 tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa cacttaaatg
     2401 tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag gtcttggttt
     2461 cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat gaagcagcat
     2521 cggtaaaatc gatccctaca acgatggctt ctgacatcac aaaattatat ggattgacaa
     2581 gaagaactcg gacagcagca atcaatgcat ctattttacc aaaaatgcta gatacggcaa
     2641 cctctacaga agattccgta cgagaagcag gggtcaatgt ttctttgatg attatgcgcg
     2701 gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta accatgcttt
     2761 ccggtccagc agcatcagtc atgggctctt tgatgtactt gagagcgtct aatggtgttt
     2821 attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga cgtccagcaa
     2881 tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat gttcgggttc
     2941 taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc gatgtcggtc
     3001 cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca gaaaaaatca
     3061 aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat tacgtcaagg
     3121 tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc aatgtccttg
     3181 gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga aaagccatcc
     3241 aagcattagc tgattattgt catacgacgg ttgaagacat cgcagaacag atcatggaaa
     3301 aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat cacttggaga
     3361 aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt acttacttta
     3421 gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt tcttctattg
     3481 gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg ccaagtaaag
     3541 aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca ggcgctacac
     3601 cagaatcgat cgaagtccac gtagaaatcg atccacagac ttcaaaagta acggctatcg
     3661 ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca acagaagaag
     3721 cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc ttattggaaa
     3781 atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct ggaagtcttc
     3841 ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca tgtatgaaaa
     3901 caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg gcagtttatc
     3961 aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga gtctctgatt
     4021 ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta tctactatgg
     4081 aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa gttagaagag
     4141 ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca gtttcggcga
     4201 gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca acaatcaatc
     4261 gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc cgatgtagga
     4321 gaaatttgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca aataaacgaa
     4381 cgaatcactt ttgcaactta tgcagaagat gaagggattc gattgatgac ggagccgtta
     4441 gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca agctttgatt
     4501 attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta tacgcaaagc
     4561 gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat tcgtgcattg
     4621 agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt ttcgccttat
     4681 gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg gatcttagaa
     4741 gaaataaaaa aaatagaaaa aagatgtacg gagtatgatt tagattacaa ataaagagac
     4801 tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acgatgttct cctgtcaact
     4861 cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga aattgttcct
     4921 tgtgactcgg agatgaacgg caggctcacg acctcttgga aaacggtgtt caaaagcttg
     4981 catctgcggt aataagtcca gccaagttaa aaacataaga agctattttt acttggctgt
     5041 tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt gtgcaatcag
     5101 ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt ttgccgtttc
     5161 tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat gggtctgtcc
     5221 tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc ctcgataatc
     5281 atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc aatcattgat
     5341 tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga aatcgtcaaa
     5401 cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc ccaaatttct
     5461 cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc aaaaaaagct
     5521 tttttatttt gagcgattac tgcctgatcg gtttttccaa cttttcccgc gtataacggg
     5581 ctaatgaagc agatcggata tcgatttttg aaacataagt gccggactgt ggaatggttt
     5641 ttacaagctc ttgattgcgc agttggatca gtgcttctcg aatcggtgta cgcccgatag
     5701 ctagttgttc ttctaacact ttatcggaga ttttacttcc aggtgctaac tcggaataaa
     5761 tgatttgttt acgtatagat tcataagctt gctgttgcaa attattgata accatttaac
     5821 cttcctttct atgcagatat aatctattat aacataaatc gcttacaaat cgtgtgggca
     5881 taaaaacaca gtctgcaaaa aaagccctga taggcatgcc tattagagcc tcgaaaagaa
     5941 aaatattgaa tcaatctggt cgtacctctt gtatcggttt gacacgaccg ccgattcttt
     6001 tagcaaaagc ttcggattct tttttgtctt tgaaaatgag cagattgtct aagttttttt
     6061 cccgagtgcg acagccagtt ttatcgatgt aaccgtgcat tactttaacg acatacatgg
     6121 ggatcacctg cttttttctt ttcattatac gaactactcg acacaataca agaaaaatgc
     6181 ttagacggtc tttattaaga atttacatca tccaaagaag cgtacgcttg gaataactga
     6241 tctaactccg gattgacttt gctcagcttc actaatcgtt gcgaatcaga aagtaaaaaa
     6301 cagtgtttac tactgccagt agtttttaat tttttgtctt cagttcccac aatttcgtag
     6361 ctaaaatcca accgtgtccc cgtatacttt tggatagttg gatggatgct cactttttct
     6421 ccgtaatgga tcatttcttt atagctgcaa tttacttcca gaacggggat gatgatccca
     6481 gattcttcga tttttttata gggaaaccct agatgtacca ataaggctac ccgagcctct
     6541 tcaaaccagc ggatataatt gctgtgatga ataatcccca tttgatctgt ttcataataa
     6601 aaaggttctc gcacataacc tgtaaatttc tccatgactc atgcctcgtt ttccattgat
     6661 tttttacaaa aataattcat ccatttcgga agtgcttcgc tgatttgtgt cggcaaaaca
     6721 atatattgtt cttttgccag ctggtctcca atgaaactat gaagataaat agctgctgca
     6781 atcgtttggt cattttgagg aaactgggct aaaaatcctg tgatcatccc agctaaagtg
     6841 tcacccatgc cgcccgttgc catagcagca gaacctaaag gattggtgta gaagtgctgg
     6901 tgaccataaa tttttgtctc atgacttttc aaaacgagca cagcaccgat ttcctgttgc
     6961 ttctttttgt tttctttatc ccgttgttct tgtatcggta gagaactcaa ccgttgccac
     7021 tccatttgat gaggtgtaag tacagtttgt tcgggatagg gtagcaaaag atgattagca
     7081 gcaagtaaac ttaaagcaga gccatcaatc accagccatt gttcttttga ttggttttct
     7141 agtacttgtt tgaaacgttc caaactctta gaagaagtac ccaatccggg accaatcaag
     7201 agtacatctg cttgctttat tacggaaaga aactggtcag tatccgtcca aactacaatc
     7261 attgcttctg gcagatgact gtgaagagca ctccgatttt tttcatcagt tactacagtc
     7321 gtgagacccg ctccgctgtg tacggcagct aaagcactca taatgattgc tccgccatat
     7381 tgttcattgc cgccaatcaa aacgatacgt ccaaatgttc ctttatgtga aatttttgga
     7441 cgaggtgtga tgactctttt cactagttgt tcgtttatca gcatctaaaa acctcctttt
     7501 actcattata gcgtatattt tttttagaga aaaacagata agaaaaggaa ttgtcagatt
     7561 ttttgaacta tttttttctg taatttgtgg tatcatgaac acgaagactt aaataagtgg
     7621 aggattaact ttatgacaat tgattggcaa aaagaagtag aagcacgtaa agaagacttg
     7681 cttgaagact tgaaaaatct tttacgtgtc aacagcgagc gcgatgactc aaaagtaaca
     7741 ccagatgcac catttggacc tggacctcgc gacgcattga aacatatgtt agcttatggc
     7801 gaacgtgatg gatttaaagt aaaaaatgtc gataactatg cagggcatat tgatcttggc
     7861 gaaggagacg aaacgctagg gatatttgga cacatggatg tcgttcctgc aggagatggc
     7921 tgggacacag atccttatga accagtaatc aaggatggaa agatctttgc acgcggatca
     7981 agcgatgaca aaggtccaag tatggcagct tactatgcga tgaagatcat taaagaactt
     8041 gacttgaaac tatcaaaaaa agttcgcttc gtagtcggca gcgatgaaga aagcggctgg
     8101 ggcgatatgg cttattattt cgaacatgaa gaagaaccag attttggttt ctcaccagat
     8161 gctgaattcc caatcatcaa tggtgaaaaa ggaaatgttt ctttagcttt acgcttcaaa
     8221 ggcgacaatg caggagatta tgtcttgaaa tcttttgttt ccggacttcg cgaaaatatg
     8281 gtgccaggaa cagcaactgc agcacttcaa gtaccaagtg cagatgcagc tatcgcgatg
     8341 gaagaagcat tttaccaatt catcgaagca aatccagtaa gcggaacgat tgaagcggac
     8401 aatacgtatg taaaaatcga attagttggt aaaggcgcac atggtgcaag cccacaatct
     8461 ggtatcaatg caggttcttt cttggcattg ttcttagata actatgaatt cctaggatct
     8521 gctaaacaat ttatccatgt agcagctgct tatgtccatg aagattttta tggagaaaaa
     8581 ctaggagtcg cttatgaaga tgaaaaaatg ggcaaattga caatgaatgc tggactattt
     8641 gcttttgaag aaaatggtac agaggaagcg aacttcatca atatgaattt ccgttttcca
     8701 aaaggtgtaa cagtagacgg attgcaaagt gacatcgaac aaacagtggg gcaagaaggc
     8761 gcaacagtta ctcgaggtgc tcgcgtaatg gaaccacatt acgtaccaat ggatgatcca
     8821 ttggtggcta cattattgca agtctatgaa gaccacactg gagaaaaagg ctacgaacaa
     8881 atcatcggtg gcggaactta cggacgtttg ttgaaacgtg gcgttgctta tggagcgatg
     8941 ttcccaggat atactgatac aatgcatcaa gcaaacgaat ttatgagtct tgatgacttg
     9001 ttcagagcaa cagcaatcta tgcagatgcc atctatcgtt tggcgaaata attaattaaa
     9061 aagaggatca agaattgcaa attatttttg cagtcttgat ccttttttta ctttctactg
     9121 tggtattcat gataaacatg atatagattc aattcatatt ctttcttagc gttcgtgacg
     9181 actgtatcca aaatcaaccc tgttactaat gaaagaagtg ctaaagtcat caatccagta
     9241 cttaaaatcg cagaagggat gcgtgtaata aagcttgtca gcatatattc gcgaatcaca
     9301 ggaatccccg tgaccaatcc aaataaaaag aagaagatgg tccaaatccc aaagaaaagc
     9361 aaaggcttat aatctttaaa cattttaata atcatcatga tcactttgaa tccgtcagag
     9421 aatgtattca attttgattc gctgccttca ggacgatcac gataatcgat tgggatttca
     9481 acgaacttga attttttatc taaagcatgg atcgtcaatt ccgtttcgat ttgaaaacca
     9541 gaactcataa taggaaaact tttgacaaag atacgattga atcctcgata gccggtcatg
     9601 acatcaagaa tttttgtttt atacattcta gtaattgtat ttctaacaag gttatttcca
     9661 aaatcgtgga aaggtctctt attctcatca aagtaggttc catttgatag gcgatcacca
     9721 atgaccatat ctgcttcacc agaacgtaat ttctcgagca aaccatgtac agcttctgca
     9781 ggataagtat catcgccatc taccattaaa taatagtcgg catcgatatc aaagaacatt
     9841 tggcggatca catttccttt tccttgacga ggttcttttt ttacgattgc tccaccttga
     9901 acggccaatt cgtatgttcg atcggtcgaa ttattatcat agacataaat atctgcttct
     9961 ggtaattctc gtttaaagtc tgcaatgact gttgaaattg ttgcttcctc attgtaacaa
    10021 ggaatcagaa cagcaatttt ttcttgttgt tccatttttc actctcctta actatagaaa
    10081 acaaaagcta aattaaaaat acctcataat caaaggaaat gctagttttt cccgctaaat
    10141 atcatttatt taaaaagaaa atactggaat atatgtttgg aaagctctat ctaaaaaacc
    10201 acccactaca aaagaaggac atgatacctc tcttttgaat agtgggtgcg tattcgttct
    10261 ctcacttttt tgacgtatta atctgttgca cctttttttc ttgaaatttc taatacggtt
    10321 cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc tattcgagaa
    10381 agacctcccc gataagcact cgaccgaacg gcgaatttcc taataggttc cttttcaaaa
    10441 gagatccaag aaccttcgat tgcaccttct tttagaaaag atgacttgat attttcaagt
    10501 acttggtctg ctgacaatac ttttttgcga tgataccaaa tcgttgaagc taccccgctc
    10561 aataatccta gaccgattcc aactgctaac ccgcctttaa aatagcttag ttcgctttct
    10621 tcatacatgt ggacatacct ccttcttctc acttgttact ccttgtgctt attttaccac
    10681 actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata
    10741 cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt
    10801 ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag
    10861 ttaacagaat tacaaggaac aagcggtttt gaacaagatg ttcgcgctta tatggaaagt
    10921 catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttggtctg
    10981 cgccatcatg aagaagcgga tgcaccccgt gtaatggtcg cagcacatat ggatgaagtc
    11041 ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcggc
    11101 tggaatcctt acgttgtttc tgctcagcgt tttacactta aaacctctac tggtaagaat
    11161 tatccatgta tttcttcttc cgttccccca catttattgc gaggaacaag cggacaaaaa
    11221 tcagtagaag taaccgatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg
    11281 agttacggtg tccttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc
    11341 aacggaaaga atattatcag taaatcatgg gacaaccgct atggctgtac aatggtatta
    11401 gaagctttag aagcgttgca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat
    11461 gtccaggaag aagtaggctt gcgtggttct aaagcttctg tcaataaatt caagccagat
    11521 ctgttctttg cagtagactg ctctgcagca gatgatacag tgacgaaaaa tggcacattc
    11581 ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct
    11641 cgtttgcgcg agtacttgct ggatatcgct gaaacaaaca atatccctta tcaatacttt
    11701 gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaacgaagg aatcccaagt
    11761 acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc
    11821 gattttgaag ctgcacgaga aatgttgatt caaacactga aaggtttgga taaatcaaca
    11881 gtgaatacga tcgtatacgg aaaataatgc gatagtgaaa ctataaaatc tggcaaaaga
    11941 ttgctttttg ccagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa
    12001 ggtgtataat ttaattgaaa aatacataaa cagaaagtag aggacaaaga atgattatcc
    12061 caaatagttt agaagaatta gcaggctacg tagaaactgg aaagagtgta tttttcttta
    12121 ctgccggctg gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa attgaaaatg
    12181 acttttctga ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg
    12241 aatggaacat ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc
    12301 gtctagtgaa taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa
    12361 caaaaggctg acatctgtta ggaggggtca agatgaatca agaatataag aatattttag
    12421 taggtatcga cggtagtgaa caagcacttg ctgctttcaa aaaagcggta gaagtggctc
    12481 gccgtaataa tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataacttta
    12541 tgggctatgc acctattgat caaaatctag tcgatcagca aactgaaaca gccaaggaaa
    12601 tgatcgaaga atgtaaagaa tatgggaaat ctgttgatta cacaaatata gaaggaatta
    12661 ttgcatatgg ttctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgagatcg
    12721 atctaatcat ggttgggcag tcaggtctaa atgcagtcga acgctttatg acaggaagtg
    12781 tagccagcta cgtgatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag
    12841 aacagccaag taattaagca agtggaactt gtacgacctt tttaatcaat taactaaaac
    12901 agtaaaggga ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc
    12961 agaagaggct tcttcggaaa taaagcatga aaaaaatttc atgcagtgaa tgcaattttt
    13021 tgttaagata aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca
    13081 aagcacacgt cggagacacc ttgatggtta ttatagccga cgataaagga acagaaaaca
    13141 ctgttgaacg caaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt
    13201 ggaacttttt ccatatctcc gatcatctaa caatcgaagg gaacggccaa gtaagcttaa
    13261 cagatgagga attagcgaaa ttgaatcgat tgatcaaaga agcaggattc gaagaacagc
    13321 ttgaagctga tcatgaaccg aagatcgttg tcgggtacgt aaaaacatgc gtcccacatc
    13381 ctgattctga ccatttatcg attactgaga cagaagtaga caatggtaca gtattgcaaa
    13441 tcgtttgcgg tgcacccaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag
    13501 ctatgatgcc agatggaatg atgatctggc caggaacttt gcgaggggta gatagctatg
    13561 gaatgatctg ttccgctcgt gaattgcatt tgccaaatgc tccagcaaaa aaaggaatct
    13621 tagtattgcc tgaggatgcc gtagtcggag aagcatttcc aaaataaaga ggttgtgaca
    13681 agtctttgtc acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg
    13741 attgatcgat cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga
    13801 ttttgacaga atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag
    13861 aaacttcttt gtcatcgatc ttagtaatga cgtcgtattg tttcaatcca gctttttcag
    13921 ccggtgtagc tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct
    13981 tcaagatttg ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta
    14041 gagctggacg tgtcactttt ccgtcttttt ccagttgatt gatgatattc actacatcgt
    14101 tacttggaat agcaaatccc attccttcta cgcttacatt agaagtagat tcagaagtac
    14161 ttgcgatctt acttgagttg ataccgataa cttgtccttc gatattaact aaaggaccac
    14221 cagaatttcc agggttgatt gctgcatctg tttggatcgc attgatattg actgtttcgt
    14281 tcgattcatt cgtacttgtc acttgtcggt tcaaggaaga aatgattcct gaagtgacag
    14341 aatttgcata ttcagatcct agtggggaac caatcgcaat cgcaggctca ccaaccttca
    14401 gagcacttga gtcaccaaaa gaggcaactg tttcgacttt gtctgaattg atcttcaaca
    14461 ccgctaaatc tgaataagca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt
    14521 tcatcaagac ttcaagacct tgctggccat ctactacgtg attattcgtg actacataag
    14581 cagtattccc gtcttttttg tagatcacgc cgcttccttc actataagct tccaaattac
    14641 tatcatctga gctgctactt tcttcttgct gcccgaatag tccgccaaaa ccgcttgatt
    14701 ggttttgact ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt
    14761 tggtgatatc agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat
    14821 tctgattgcc gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa
    14881 tgccagctgt cagtagtcct ccaacgatcc ctccaacaag ccccagacct aacttacgcc
    14941 agaggctatt atttttactt tttttcatct ttggtgtcac attttttcta tccatgtgtt
    15001 cctcctccaa ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc
    15061 tactttgaga ctgtgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga
    15121 aaatatttga ttttcttttt ccacagatac tgtgtaaaac tacgcggata ggattggaaa
    15181 aataagggat aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt
    15241 atgtttgcct aatgaatgtg atgaaataag gtaaaataac actgttttct gtggataaac
    15301 ctgtggatgt tgtgcataac tgttggggaa atctttttaa caaaaatagc caaaataaac
    15361 atatagacga aaaaattgat tataataaac aaagtgagca tgaagaccaa aggagagaaa
    15421 acatgaatat caaaattatc tcagtaggaa aattaaaaga aaaatacctg atccaaggta
    15481 tcaatgagta tgtcaaacgg ctgaatgcct atgcaaaaat cgaattgatc gaagttcctg
    15541 acgaaaaagc cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag
    15601 gggaacggat cttagcgaaa atcaaagaac aagaatacgt ctatgcctta gcaattgaag
    15661 ggaaaaatcc tacaagtgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa
    15721 aaagccacct tgtttttgtt atcggcggct ctttaggact cagcgaagcc gtcatgaaaa
    15781 gaagcaatgc tcagatctct tttggaaaaa tgacgtatcc acatcaattg atgcgtctga
    15841 tccttgtcga gcaaatctac cgtgccttcc ggatcaatgc gggggcgcct tatcataagt
    15901 gatgcggttt ttatcattga tcctaaaaat cttagcaaga caaaaagtac gtaagaaagt
    15961 aaagactaaa tacttcggag gaacaaatga ataacaatga taggttattg cgattgcgtt
    16021 atgcgattga tttgaaagat acggatttga ttaaagcttt tgagctaggc ggtgtaccac
    16081 tgactaaaga agaagctcag gcggtactga ccaaagtaca ggataagaac aaagataatg
    16141 cagaaaataa tgtgtatgaa aaaaccatca acaaccaagt ttttgacgca ttcctaaatg
    16201 gcctgattct cttggcgcgt ggtccgcaaa aagataagcc gatagtagac acgccgcaaa
    16261 aaagcaagga aatcaaatat atcaacaatg tgttgctaaa aaaattaaaa atcgccttgt
    16321 ctatgaccac tgatgatatt ttagacgttt ttgctgaggc agaaatctat ccatcaaaag
    16381 gcgaaatcgg ggcatttttg agaaaagaag gccaacgaaa ttttaagcct tgcggagata
    16441 aatatatgcg taattttctc aaggggttag gaatttataa tcggagaaaa gtataaagaa
    16501 atttcatgag aaatcaaaac aatggttgtt ttgatgagag ctagtttttg tatgagatta
    16561 aaatattatc ttaatcgagg agctggattg gttttatgaa tgatgaatat aagaatgatg
    16621 aagataagat gctttttgaa gagatagaga atcgatgtag gctcaatttt gagcttcggg
    16681 gaaagatgtc tttaatacaa caaaaaaagt atttagcgaa taaatcagag tttactctgg
    16741 gacatgttga aaaattaatc tcggattgga ttagtagtag aagcgaattt acaaaaataa
    16801 aacagccgat taaatttgac atgaaaaaac ttttgttaaa taagagtgag ataggtaata
    16861 gagatcaata tattagagct aaagggcaag aaataattga ttcattaggt gaaatgaggt
    16921 catacaatta tttgtatgta acacatagag ctgatggaat ggtcattact gtggggaaat
    16981 cttcttctaa tgatattttt ttagatggag atctttttta tcaactcaat acaaatcact
    17041 tgtctggcac tgaaaatata attttaagga cggaatatgg caatgaaata tttgcaaaat
    17101 atgatgagat tttaaaaaat tatttggact gggcttggat aattccggtt gagtcaggag
    17161 atgctaagaa actggagaga ttattgggcg atgaactaat taataaaaaa gtaccaattt
    17221 taaattatta ttcccataga caataaaagg ctatttttca tgataatact agttataact
    17281 gtaaaaatgg aagaaaatct gggaaagaag tttttaaata agtgtattgt tcgattttaa
    17341 taaataataa gcaataccat ccgtctgtgt tgcgcaacat cgtactacaa tgaatatttt
    17401 ttcacaaaaa gaaaatcatc catcgaatga atggaaggtt ttagtataca cgaggttaat
    17461 gccccctctt ttataatcag aggagtgagg ttttttctat gaatgtttct attttcttat
    17521 tgctagtcta gtttctctta cgatgaaatt gggattctat tccagttttt attgatttgc
    17581 tgataaaata acttgaatgg tggatataaa catgataaat aagagaagaa taacaaaaca
    17641 aggttgtgaa atgattctca aatgaatata atagaaagaa aaaggaggaa gaatcaatga
    17701 aaatagcaat tgcaggcgct ggcgcaatgg gaagtcgctt tgggttaatg cttcaccaaa
    17761 gtgggaacga agtattatta atcgatggct gggcagaaca tgtccaacaa atcaaagagc
    17821 atggattaca agcaaatttt aatggtaaag aggtagaagc aaaactacca atcgttcttc
    17881 aatccgaagt agaaaaagaa gatcaagttg atctgattat tctatttacc aaagcgatgc
    17941 agctggaaaa aatgctgcag gatatccaat cattaatcaa aaaagataca gaggtcttat
    18001 gtctattaaa tggtatcggg catgaagata ttattgagaa atttgtacca atggaaaata
    18061 tctatattgg aaataccatg tggacggcag gtcttgaagg tcctggtcag gtcaaattat
    18121 ttggaagtgg ttcagtagaa ttacaaaatt taggtgatgg aaaagaagca gctgcaaaaa
    18181 aattagcaga caaattgtct gaatcaggat taaatgctca tttttctgac aatattcact
    18241 attctattta tcgcaaagca tgtgtgaacg gaacaatgaa cggactatgc acaatcttag
    18301 acgttaatat ggctgagcta ggaaaaacat cgactgctca taaaatggtg gcgacgattg
    18361 tcaatgagtt tgccaaagta gcagcagtag agaagattga actagatgtc ccagaagtca
    18421 ttgcacattg tgaatcttgt tttgacccgg aaacaattgg gttacattat ccttcaatgt
    18481 atcaagactt gattaagaac catcgattaa cagaaatcga ttatatcaat ggcgcaattt
    18541 ctagaaaagg gaagaaatat ggtgttgcga cgccttattg tgatttcttg actgagcttg
    18601 tccatgcaaa agaagatagt ttgaacgtaa aataaaggag agattgaatg gaaacttcac
    18661 aaaaaatatc accgaaaatt tttctgaata aagtcctcgc tggtacagca acaggaatca
    18721 ttattggatt gattcctaat gcagtattag gtgccatttt aaaatatttt agtgcgtatc
    18781 cctttgcggt aacgatcgca cagttagcag ttatttttca actagcaaca cctttgatca
    18841 ttggtggttt gattgcctta caatttggat ttgatccgat gagaatgatg gttgtagccg
    18901 gagcttcttt tgttggttct ggtgtagtca aattcacacc tgatttgggt gaaacgggtg
    18961 tttacgttgg tgctggtaca ggcgatatta ttaatacgat gctgacagca tcgattgctg
    19021 tcgggctgat tttactgatt ggaaatagtt ttgggtcagt gtcgattgtg ttaacaccga
    19081 ttgttgtagg tgtaggtgca ggtttacttg gtttttattt attccctttt gtaacagcaa
    19141 ttacttctgc aattggatca ttgattaata acttcactac cttacaaccg ctgttaatgt
    19201 ctatcttgat tggttgttcc tttgcctttt tgattatctc tcctatttca acagtagcaa
    19261 tcggaatggc aatccaacta gatggtgttt ctgctggtgc ggctgcgatg ggcgtagctg
    19321 caaccacttt tgttttagta gtgaactctt ggaaggttaa taagtcaggt gtgacaattg
    19381 cgattgcttt aggcgctatg aagatgatga tgcctaatct atttagaaaa cctgtgattc
    19441 ttattccttg tttagttagt gcagtgatta cagctattcc tgtcgctcta ttttctattt
    19501 caggtacgcc tgcttctgct ggtttcggcg tggtcggact tgttggtccg ttggcttctt
    19561 tagatgcagg gctaaatggc tttcttgttg ttctttgttg gttagtcatc cctgtttttg
    19621 cggcactaat tactcaattt ttatgcgaga aagtatttaa actgtatgat cgagaagaag
    19681 tcttcaaatt tttaggctag tagattttac taagaaattt tcaatcaatg gatgcgtaag
    19741 tgagatgtca acaacatctg acttacgttt ttttgccttt ttgagtagga gattgatgct
    19801 gacaactgaa ctcttcagtg ccattcgatt ttttaaaatg tagaagaaac tcacctttat
    19861 gttattgatt gttcaaaagg caactagaat aaaaatatgt gttaaaatga gaaaaaattt
    19921 gattgttgga tgtatcgaat aatcactaaa aagtaggaga agaaaatgat ttttttgcaa
    19981 aaatatggtt tttatttttt attattaggt gtgattagtg atttatcgac accgtatatc
    20041 cttggccttt tttatcccaa actgaatcaa atgactaccg taattagtgt gtttggtgac
    20101 gtagatagtc cagtcagacg tgctttcttg gtctggtcag ttgtatctgg actttttttt
    20161 gttctttcgt tgcctgcgtt gtatcaccta tttattggaa catcaaaaac acttgcaata
    20221 cttgctgttg caacagttgg tttatatggg ataggtgatt gtatttttac tgggttattc
    20281 agtatcaata cgaatgaaag ctcttggaat ctatcgactt ggatccataa tataggttct
    20341 gggctaggct atgcaggctt tctacttttc ccattgttac ttgttctgct ttatcgtcaa
    20401 agtggtcaag gtacgcttag ccatttttat cttgttttaa cagttattag tttactaatt
    20461 gcaggtcttt acggattagc acggattccg tcgattagtc agtttgcctt ctttaagcaa
    20521 ttgggcttct ttcaacgctt aagttttttc tttaactatt tgccgatgat ttgtttaagt
    20581 ttttttcaac tacgaaagta gaaaaagcag tgtgaagctg attaagtaaa agaatttctc
    20641 tatgggtaac cattcgtgtg gatgtgttcg actaagaata gagatgagga gagcgaaaag
    20701 caagttagtc ttaacttgtt atgcgttgtg ctaaatttca ggtagtcatt ttttgaatga
    20761 ataagaagaa aacttgaaag agggtgactt catgttagat gtggagcaga tggaaataga
    20821 cctttataat attattttaa atcgaaatac aagagaatgg gaaagaacac aatttgaaga
    20881 ggcaaaagaa gacctagcaa aaatgtcgat aaagcaggtg atagaaaaac tagaggtcaa
    20941 cttgcgtccg ctagcactac gagataattt gacaccagat gcgatggatt tttatttaag
    21001 aatcacaggg gagtcagcga gtgcaaaggc ttatgattat gccaaacata agataacttc
    21061 acccttatac caagaacggg cgatttttgc cggtggctgt ttctggtgta tggttgagcc
    21121 ttttgaaaca aaaccaggtg taatctcagt gttatctggt tatacaggtg gagagaccaa
    21181 aaaaccaaat tacgatcaag tcagtggtgg atatactggg catgttgaag ctgttgagat
    21241 catatttgat acacgtcaaa taacctatga agagctgttg gagatttatt ggcaaatttc
    21301 agaccctaca gatgcctttg gtcaatttca agatcggggg aaacaatatc gttcaattct
    21361 atttgttgtt aatgaaaagc aaaggcaatt ggctgaaaaa tcaagagaac aactgattca
    21421 atctaaccga tttagccgtc ctatcgttac agagattcgc caagcaaaga ctttttggcc
    21481 agctgaaaat catcatcaac aattttatca gaagcaacct aaaagatata aaaagatcaa
    21541 gcattcgcat cagcttcatt tattttttca gcgacataga tagaggaagg ttgtggcgca
    21601 aagcgttcag ctccgacaaa ataatgagca actgacaaaa atagcttttc atatttttgg
    21661 acagttatga attatttcga ggagttgctt tgagaacacc gaggaaaagg ttgtggcgca
    21721 aagcattcag cttcaagtcg caaggaaaca ggaggtatga caagtctttt gtcacaactc
    21781 ctgttttttt actagccttt atttccatgt gaaagagaat tcgaattgta gaatcttgat
    21841 ttttctatgg aattttgtag agaatcatct tgataattaa tagacaccta atcgaattcc
    21901 acattaatga tgaactaaat tccgtataaa agcgcaaaca ataacta
//
LOCUS       DASJRQ010000046        21573 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJRQ010000046 DASJRQ010000000
VERSION     DASJRQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.046"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(567..1592)
                     /locus_tag="U4A80_001538"
     CDS             complement(567..1592)
                     /locus_tag="U4A80_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1975164.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1867..3552
                     /locus_tag="U4A80_001539"
     CDS             1867..3552
                     /locus_tag="U4A80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1975165.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3566..4849
                     /locus_tag="U4A80_001540"
     CDS             3566..4849
                     /locus_tag="U4A80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1975166.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4919..5788
                     /locus_tag="U4A80_001541"
     CDS             4919..5788
                     /locus_tag="U4A80_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1975167.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5785..6621
                     /locus_tag="U4A80_001542"
     CDS             5785..6621
                     /locus_tag="U4A80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1975168.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6647..7714
                     /locus_tag="U4A80_001543"
     CDS             6647..7714
                     /locus_tag="U4A80_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1975169.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7711..8538
                     /locus_tag="U4A80_001544"
     CDS             7711..8538
                     /locus_tag="U4A80_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1975170.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8583..9608
                     /locus_tag="U4A80_001545"
     CDS             8583..9608
                     /locus_tag="U4A80_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1975171.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9605..11788
                     /locus_tag="U4A80_001546"
     CDS             9605..11788
                     /locus_tag="U4A80_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1975172.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11788..12918
                     /locus_tag="U4A80_001547"
     CDS             11788..12918
                     /locus_tag="U4A80_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1975173.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12915..13592
                     /gene="pgmB"
                     /locus_tag="U4A80_001548"
     CDS             12915..13592
                     /gene="pgmB"
                     /locus_tag="U4A80_001548"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1975174.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13676..14203
                     /gene="msrA"
                     /locus_tag="U4A80_001549"
     CDS             13676..14203
                     /gene="msrA"
                     /locus_tag="U4A80_001549"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1975175.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14404..15090)
                     /locus_tag="U4A80_001550"
     CDS             complement(14404..15090)
                     /locus_tag="U4A80_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1975176.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15170..16708
                     /locus_tag="U4A80_001551"
     CDS             15170..16708
                     /locus_tag="U4A80_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1975177.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16828..17295
                     /locus_tag="U4A80_001552"
     CDS             16828..17295
                     /locus_tag="U4A80_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1975178.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17814..18257
                     /gene="rplM"
                     /locus_tag="U4A80_001553"
     CDS             17814..18257
                     /gene="rplM"
                     /locus_tag="U4A80_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1975179.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18273..18665
                     /gene="rpsI"
                     /locus_tag="U4A80_001554"
     CDS             18273..18665
                     /gene="rpsI"
                     /locus_tag="U4A80_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1975180.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18753..19979)
                     /locus_tag="U4A80_001555"
     CDS             complement(18753..19979)
                     /locus_tag="U4A80_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1975181.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20051..20197)
                     /locus_tag="U4A80_001556"
     CDS             complement(20051..20197)
                     /locus_tag="U4A80_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975182.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            20889..>21573
                     /locus_tag="U4A80_001557"
     CDS             20889..>21573
                     /locus_tag="U4A80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1975183.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKA"
BASE COUNT         7012 a         3824 c         4468 g         6269 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttttttaa gaagctatta attattgata
     1741 cataagcttt cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga
     1801 cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg
     1861 aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa
     1921 accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc
     1981 aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca attttttgaa
     2041 gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca
     2101 tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt
     2161 gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga
     2221 agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg
     2281 tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg
     2341 gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt
     2401 gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg
     2461 aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa
     2521 aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact
     2581 tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat
     2641 gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc
     2701 agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct
     2761 ttgacccttc aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg gttaaaaacc
     2821 atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc
     2881 ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag
     2941 ctgaagaaag agggtacccg agtaaactac aagaaaaacc cagatggaac gaccagtcca
     3001 tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg
     3061 agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca
     3121 atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga
     3181 gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag
     3241 acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa
     3301 aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt
     3361 atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat
     3421 caatcagcag gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt
     3481 atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa
     3541 ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc
     3601 tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac
     3661 aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact
     3721 ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga
     3781 atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat
     3841 ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc
     3901 agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct
     3961 tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg
     4021 tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga
     4081 tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc
     4141 tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa
     4201 tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga
     4261 agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac
     4321 agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat
     4381 tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca
     4441 aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga
     4501 gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga
     4561 aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact
     4621 ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc
     4681 tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat
     4741 gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa
     4801 cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct
     4861 gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat
     4921 gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat
     4981 gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt
     5041 cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga
     5101 aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt
     5161 agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt
     5221 cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc
     5281 tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt
     5341 agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag
     5401 cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt
     5461 tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc
     5521 aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac
     5581 tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac
     5641 gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct
     5701 tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct
     5761 ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac
     5821 gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca
     5881 tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg
     5941 acttttgaac attatcagga tattttcaat gctgagattt ttccattcat catgtatttc
     6001 aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc
     6061 ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt
     6121 tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc
     6181 ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt
     6241 cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa
     6301 gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg
     6361 gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat
     6421 ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac
     6481 acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc
     6541 gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc
     6601 gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt
     6661 tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa
     6721 cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt
     6781 tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc
     6841 aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat
     6901 tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt
     6961 ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact
     7021 acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt
     7081 cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg
     7141 tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat
     7201 cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaatacg gtgccgatta
     7261 cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg
     7321 tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaag
     7381 agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg
     7441 gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc
     7501 tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg
     7561 ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt
     7621 tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa
     7681 aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt
     7741 ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac
     7801 gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa
     7861 acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc
     7921 gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag
     7981 gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg
     8041 ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt
     8101 tatttagata aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga
     8161 taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa
     8221 ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt
     8281 tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat
     8341 cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa
     8401 aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa
     8461 atttatcagg aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat
     8521 gatttagaga aagaatagag aaagaataac aataagtaaa taagcaaagg agggagaaat
     8581 aaatgaagaa agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt
     8641 attatcaaaa aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc
     8701 gagcagaaga cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa
     8761 tgctgaatga gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg
     8821 aacatgtatt ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta
     8881 tgacagctga tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg
     8941 cttatggatt ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg
     9001 ttctcgggga tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct
     9061 ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc
     9121 acatgcttga tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt
     9181 acagcttcca aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa
     9241 gcaaattcac agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca
     9301 tccaactgaa tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt
     9361 gtggcgatca ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa
     9421 agctggtttc cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc
     9481 attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat
     9541 atgtcatcca gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta
     9601 ccttatgaaa gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca
     9661 gatgaccatt ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa
     9721 tcaagtaagg ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc
     9781 cgagttagtg aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt
     9841 ttctcttgag acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga
     9901 gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg
     9961 tcgtgttgct gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt
    10021 gaccgaagac tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg
    10081 tacacagcag ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc
    10141 ctaccagaca acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg
    10201 tgtattccaa gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa
    10261 tacgtccttt acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc
    10321 gtttaagaaa agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca
    10381 gtcgatgtct tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc
    10441 ttctttcgat cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg
    10501 caatgatcca agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca
    10561 tgttttctgg gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt
    10621 agcaaagaat ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag
    10681 agattatcac tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga
    10741 gacacccaaa tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc
    10801 tgccgcagaa catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc
    10861 aacagaggat caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc
    10921 tttttggctg agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg
    10981 tccagatgaa tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta
    11041 taatgtggcc ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca
    11101 aaatttttta gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga
    11161 tgacacgtat ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag
    11221 ccagtctatt ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca
    11281 agcagatctt gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc
    11341 taagaatttg cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat
    11401 tcacgccata gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga
    11461 agcgtgcctg attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc
    11521 ttcattagga gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa
    11581 tggaactctt tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt
    11641 tatttggaaa aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa
    11701 agagacgaaa gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga
    11761 actgatcttg gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa
    11821 gaaagtctga cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt
    11881 gttcaggctg aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca
    11941 ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa
    12001 aacaaagaaa tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc
    12061 gtcggattga aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga
    12121 gtaggagaac tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt
    12181 ggcggaagta gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt
    12241 tacgatcaag ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca
    12301 gttgataaaa cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat
    12361 gttactaatc ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc
    12421 ttgaaagaaa atgaattagc agtggctgac caagccatgc atgattttta tatgagattt
    12481 gcccctcaag taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg
    12541 ttagcatttg cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag
    12601 ttcgataaac gggtacagca agcagacctc gtaatcgtag gcgaaggacg tctagatgga
    12661 caaagtctaa aaggaaaagc gccagttggg attgctagaa gagtaccaga gggcgttcct
    12721 gtgatagcta tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga tgttggcatt
    12781 tcggccgttt ttccgatcct tcctgcagct gtgcctttac aagaagcttt acagcaagcc
    12841 actgataatc ttcatcgcac agctaaaaac atcgcatctt tacttcaagt cgttcatttt
    12901 caaggaggta atcgatgaaa atcaaagcag cactattcga tttagatggc gttcttgtag
    12961 acacagctcg ttatcattac gaagcgtggc tggttttagc taaccagctg tccattccat
    13021 tcactgagaa agaaaacgag caactgaaag gaatatccag aaccgagtca ctagagcgat
    13081 tgctgtcatt tggaaaaatg gaacagaaat tctcagaaaa agagaaatca gcctttgctg
    13141 agcaaaaaaa caatctatat cttcaagcga ttcaaaaaat ggatgaaaca tccgttttgc
    13201 ctggtgcaat agctgttctt gagtacttga aaaaaacaaa catcaagatc ggattgggat
    13261 cagcaagcaa aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt tattttgatg
    13321 tccttattga cggcacgcaa gtatcaaaag caaaacctga tccagaagtt tttctcaaag
    13381 gagcacagca attgaatgta ccgccaaatg catgcttggt cattgaagat tcagaagctg
    13441 gttgtcaagc agctttggct ggtaacatgc atgtattggg aataggtgaa aacataaatc
    13501 ttccttctgc tgaatatgtg attcctgatt taaccgtttt tgatcaagta cgttcgtttt
    13561 ggcatttatc agaagccgta tcacgtatat aatagtttcc gttttttctg tcacaagcaa
    13621 aacgttatcc tccttttgga aatcaaggta tgatgagaaa aaagggggat tttatatgac
    13681 aaaaaaagca gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat ttgaagagca
    13741 gccagggatc atttctgtga catccggtta tactggcggg catgtaccaa atccaactta
    13801 tgaacaagta acaacaggca caacagggca tacagaagca gtagagatcg aatatgatcc
    13861 agaagttatt tcttatgatc agttggtgga aatctattgg cagcaaaccg atccgactga
    13921 cgcattagga cagttcgctg atcgcggtga ttcttatcgt ccagtgatct actataatga
    13981 tgaagaagaa cgaaatattg cagagcgttc aaaacaagca ttgcaagaca gcgggcgatt
    14041 cgataagcca atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg cagaagatta
    14101 ccatcaggat tattacaaga aaaacaagct ccattacaat ctttatcgag aaggttctgg
    14161 acgtgcaggt ttccttcgtc gacattgggg cgtcaagaaa taagaagccg aaaaagctac
    14221 ttatggaaca ccgtatatca gaggttgtaa aaccagcatt ttgagctaat actgagggtc
    14281 agctgattga acaccattta gtggagaggt tgtgaacctg tcgcctagct ccaagtcaca
    14341 aggatttggt aagagaacac tatatatcag aggttgtgac aagtcgtttg tcacaacctt
    14401 ttttcaatac ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag gaatgaaagg
    14461 cagcttttcc aacttttttt tatgacagat aaagtaaaca gaaaaaacgg atattccaaa
    14521 cagactagca agaaacaaga tctgaagcaa ctcttctaga gacaagccac cactccaaga
    14581 aagcatcaac agagtatcgc cagctccgag ataggattga ccatagcgaa ggcacacacc
    14641 gcctacgata aacgttaaca gcacactttg ccattggaag acgccggcct gcaaccaaaa
    14701 aatccataaa attgcccaag aacagaatag cgtgtgagaa tggaccatga agtaaaataa
    14761 atccatcaac gaaaatagca aagccgaaag catccatatg aacgtcgaaa aatttcttgc
    14821 ggaagaaaaa gtaaagaacc aggcaaaaag accaccacat aaggcttctg ccaacaaata
    14881 gtagaagggc aaccgacacc tgcaatagcg gcagcgaaat cgttggacga tgatcgagag
    14941 aatcggaatc aattcatacc aagaaagcga acgacagcat ttgacacaat gagatctagg
    15001 ataaataata gagtgaccta acggaatccg ctgggcggtt aaacataaaa atgatccgat
    15061 acagcaacca atgataaaat aaagtaacac gccatctccc ccttttcaaa accaagatac
    15121 gcaagcttaa actaaatttt tgtacataca taaaatttga ggtgaaaaaa tgaacgtttc
    15181 aatcggatta gatatcggaa ccacacaaac caaagcagtt gctttcacag atgaagccca
    15241 agaagcagca tccgcttatt ttcgctaccc tttgatccaa gaaacacaag ggatggcaga
    15301 acaagatcag gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag taattaaaca
    15361 gttgccttca gacacaatca ttcaatttgt ctccttctcc tctgcgatgc atggcctgat
    15421 attgttggat gaagagaaaa caccgttatc tcgaatgatc acttgggcag ataaccgagc
    15481 agcggacgaa gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt atcggcagac
    15541 tgggacgccg attcatccaa tgacaccatt gaacaaaatc aagtggctgc aaaacagcca
    15601 acctgaatta ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata tcttttatag
    15661 attgtttggc gaactgatca gcgactacgg aacagcttca ggaactggat attttgatat
    15721 ccatgaattc caatgggcag aagaagtgct tgactacttg aacattcgaa tagagcaatt
    15781 gccgcaagtc tttccttcca cctatcaagt aacaggtcta ccatcaaaaa cagcagcaga
    15841 actaggtttg ccaaatgata tatcatttgt tttgggaggg gcagacgggc cgctttctaa
    15901 tctagggtta ggtgcttttg gtgatacgat cgccgctttg accgtaggaa cgagcggagc
    15961 acttcgtttt attactactc agcctcagtt ccatccagaa atggagacgt tttgttatgt
    16021 tctggatcag aaacattggg tagtgggagg agcgactagc aatggtgctg gcatctttga
    16081 ttgggcaggg catacatttt tacaggaagt cgttcaatct gctaaagaaa aaggagaaaa
    16141 tccatatgat gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca atggtctgat
    16201 ttttcatccc tatttacttg gtgagcgagc accgctttgg gacgcagatg cgtttggcag
    16261 ttttatcggt ttacagcggc aacatacaga aaaagaaatg atgaaggcga ttctagaagg
    16321 gatctgtctc aacctttatc ggattttaaa aggagtttgc caggaaaatc agctcacaga
    16381 gatccgtgct acaggcgggt ttgctcaatc tccgattttt cgtcagatga tggctgatgt
    16441 actgggttac ccgcttgttt tccctactgt cacagaagca tccgcattag gagctgtgat
    16501 tttaggctgg agaagcatcg gaaagatcca aagtatcagt gaagcacagg aattgataga
    16561 attgaaagag caagttggag tacaagaaac tgatcataaa aaatatcaga aaatctatcc
    16621 attatttgtt tcgacacaaa agcatttagc acagacttat cagcaatttg ctcaactgcg
    16681 agaagaatta gctgattctg aaaaatgatt ggctttacgg ataaaaaagc gaactgaaaa
    16741 tcgttctcag gtaaatgatt ttgttgtact tttcattgaa agtctactat tattttaagt
    16801 gtaagaaaat gttaggaggc aagaaagatg aaattccaac aaaccaaaga agtattaaat
    16861 caattagttg cagatctaag ccaaatgtct gttgttgttc accaaacaca ttggtacatg
    16921 agaggaccag gtttccttac attgcaccca atgatggatg agttcatgga tgatttgaac
    16981 gctcagttag atgaagtatc cgaacgcttg atcacattag acggttcacc ttattctaca
    17041 ttgcgtgaat ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg ggatcgtact
    17101 attccagaac gtttggaaac acttgttgca ggctatcgtt atctagcaga tctttaccaa
    17161 aaaggaatcg aagtttctgg agaagaaggc gacgatccaa cacaagatat ctttatcgaa
    17221 ttcaaaacag ccacagaaaa acgtatctgg atgatccaag ctcatttggg caaagcgcca
    17281 gagatcgacg cgtaaaaact gttgttgaac ttgaccgaaa aagaggttgt gaaatcagcg
    17341 ttttgacctg agtattagag aaacaaacgt caaaatagtt cctcatattt tgccgttttt
    17401 tgagctaata ccgagggtca gctgattgaa caccgtttac caagaggttg taagcctgcc
    17461 gttcagctcc gagtcacaag aaacaatttt aaaaaacagc ttcttatgtt ttatggatat
    17521 tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcaaaata aggggttgtg
    17581 acaagacttt tgtcacagcc tctttttgtt tttcattttg acaggaaacg acccttttat
    17641 ataaaagaaa gaaaagaagt aaagtttcat ataaattttt caatcaagca gaaaaacgtt
    17701 gacattgact agattagcgg gtaatatgtt aaatggtatt gtttgcccca caaagagccc
    17761 cggaaactcg aagtgtatgt caaacatcag aacttaaatt ggaggaaaat aacgtgcgta
    17821 caacatatat ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta gacgcaactg
    17881 atgttccatt gggacgtctt tcaacagttg ttgcatctgt actacgtggt aaaaataaac
    17941 caactttcac acctcatttg gatactggcg atttcgtcat cgtcatcaat gcagacaaag
    18001 tgaaattaac aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc aactacccag
    18061 gaggattgaa atcaatcact gctggcgaat tgcgtgctaa aaattctcgt cgtttgatcg
    18121 aaacttctgt aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa tttacaaaat
    18181 tgaacgtata cggtggagca gaacatccac atgcagcaca acaaccagaa gttttagaca
    18241 tcacaaactt aatctaatag gagggaattt cattggcaca agttcaatat atcggcacag
    18301 gccgtcgtaa aaacgcagtt gctcgcgtac gtctagtacc aggaactggc aaaattactg
    18361 taaataaaaa agatgttgaa gaatacattc cacatgctga cttgcgtgaa gtaatcaatc
    18421 aacctttcgc tgttactgaa acaaaaggcg catatgacgt atttgttaac gtaaacggcg
    18481 gaggatacgc tggacaatca ggagctatcc gtcatggtat cgctcgtgca ttgctagaag
    18541 ttgatcctga cttccgttta gctcttaaac gtgctggctt acttactcgt gacgcacgta
    18601 tggtcgaacg taaaaaacca ggtcttaaga aagcacgtaa agcttcacaa ttctcaaaac
    18661 gttaatatcc tttatatatc aacgtttctg gcacttttca attatttgga aagtgcttct
    18721 tttgttttta agaacacatt taagaacaca tatcaatcac acttattcaa tcctctctga
    18781 aaagcttcaa aaacagcctt atcaactata tctggtgtgt tcacatatat ctttgttgta
    18841 gaaatatctg agtgtgttaa agcatttgaa atttgattca tatctgctcc accttgtcta
    18901 gctatagttg cataagtatg acggagtttg tgtggagata aatgaactaa gtcaggatgc
    18961 cttcttttaa ttgaattgat tcgatagttt aggtaatcgg catgaacagg acaatttact
    19021 tctccagatg gtttagtata agtaaagaga tactgatcta acgtttgctt aatttttaat
    19081 ttcaacaatt gatcagcttg taccgatttc cattcactta gcaatgtcat tacaacttct
    19141 ggtacttgaa tgacggtatc ttttcgacct tttgtaaact tttttctttg atatttatct
    19201 aaagtatgtt taagacggac cgtttgtttt tcaaaatcaa tatatttcca ttgcaaagcg
    19261 taagtctcac tttttctatc accaagatac agggtaatca taaaaagtgt gtaatcatga
    19321 aaagtaagta aatgattatt aaaatcatca tttactgctt ctatccaatt agtaagttct
    19381 tttgatgata gtgcttgctt accttcttgt attctttttt cttctaaagc atttttttta
    19441 ggtgcagtga tactttgaat aattttagta gttcgattaa attcgatata atttagtatt
    19501 tctgcaatat cgaacatgga acgaacatat ccttttatga tttttatatt tgcatattct
    19561 ttcgattttt tggttaattc atctgatatt atctcagtat tgatatttag ataattcatt
    19621 gcgtattcac caaacatagg taaaatatgc aaccgaaata tatcttttgt attactaatg
    19681 gttatatcac taggaggcct gtttgatcta attgtttgac ctaattcata tctaggaagc
    19741 catttagatt cgtaaaattt tttaaacgtt atttttcctt ttaattctaa tgagtttgca
    19801 ttctcctctc tcaaaacttt ttctattttc ttcttcatat cattttcggc tttaatcgcc
    19861 aattgcctat ttggaaatgt tttttgatac ttttctgatg caattcctgt tatttcttta
    19921 aacctttttg gatatctcag attagtttta tatttcccat ttgaaagctt tgtaggcaaa
    19981 atcctcactc cttaaaaaaa gtaaggcagt atatttcact aattttagta tatgcggcga
    20041 gaagaaaatt tcaagtattc ataaagtctt taatatcttc tacgtcaaag cgaactacac
    20101 caccaactct tattactggg aaattatata tacgaatcca cttatctaga gtattattat
    20161 cgattcctag aaatgcacag gtttgttttt tattcaggta gactctggat tctataaaat
    20221 catctcctaa aatagtagtt atgaaatttt tgaattgctt tgggtaagca cataggaatt
    20281 tcacctctcc ccagtttacg gcgagcctta cggaagtatc attatttgct gctattgtca
    20341 tggcattttg ttttcccaga acaaaaagaa atctctaagt atttgtcgct aatcaggtca
    20401 tggcgtactt gagtttctag ctcgaatagc agggatcgtt attcaaaaat tgtatttaat
    20461 tgtcaatgat caaaaatagt ttttttccta ttcactatta ataacgataa acatgtataa
    20521 aaagacacac tacgaaaaaa gttaaatgga gtttactgat tttcatattt gtatatggaa
    20581 cagacatagt cttgaataat ttttataaat gttttgatgc ttatcaactt attcaacccg
    20641 ctatccactt caagtataat ttttccatac gccaataaag tagttttagg agcatgttct
    20701 catttgagaa cgctcctttt ttatatattt tgaaagctac ttactatacg cgtagtttca
    20761 aatagttttg tcattctaat cttcagcaac agaaaaaaat ctaaactata aaatttaggg
    20821 ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc atttagaagg
    20881 agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt ttagcattga
    20941 acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt ccaaatggtg
    21001 ctggaaaatc cacattgctt aaagcaatgt taaatattat ccctcatcag ggagaggtct
    21061 ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta gagcaaaaag
    21121 ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg ggaacatatg
    21181 ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt tcaaaggctt
    21241 tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta tctggaggac
    21301 aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc atatttttag
    21361 atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat acgttaaaaa
    21421 aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc aaggtaaaga
    21481 agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga agtgtagaat
    21541 ctgtatttaa tgaagaaaat ttaaaaaaag cct
//
LOCUS       DASJRQ010000047        20588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJRQ010000047 DASJRQ010000000
VERSION     DASJRQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.047"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="U4A80_001558"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="U4A80_001558"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1975184.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="U4A80_001559"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="U4A80_001559"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1975185.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="U4A80_001560"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="U4A80_001560"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1975186.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="U4A80_001561"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="U4A80_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1975187.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3502..4482)
                     /locus_tag="U4A80_001562"
     CDS             complement(3502..4482)
                     /locus_tag="U4A80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1975188.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4506..5990)
                     /gene="galT"
                     /locus_tag="U4A80_001563"
     CDS             complement(4506..5990)
                     /gene="galT"
                     /locus_tag="U4A80_001563"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1975189.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6002..6991)
                     /gene="galE"
                     /locus_tag="U4A80_001564"
     CDS             complement(6002..6991)
                     /gene="galE"
                     /locus_tag="U4A80_001564"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1975190.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7239..7406
                     /locus_tag="U4A80_001565"
     CDS             7239..7406
                     /locus_tag="U4A80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1975191.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7468..9279)
                     /locus_tag="U4A80_001566"
     CDS             complement(7468..9279)
                     /locus_tag="U4A80_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1975192.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9276..9641)
                     /locus_tag="U4A80_001567"
     CDS             complement(9276..9641)
                     /locus_tag="U4A80_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1975193.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9804..10199)
                     /locus_tag="U4A80_001568"
     CDS             complement(9804..10199)
                     /locus_tag="U4A80_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1975194.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10217..11179)
                     /locus_tag="U4A80_001569"
     CDS             complement(10217..11179)
                     /locus_tag="U4A80_001569"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1975195.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11179..11391)
                     /locus_tag="U4A80_001570"
     CDS             complement(11179..11391)
                     /locus_tag="U4A80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1975196.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11412..12110)
                     /locus_tag="U4A80_001571"
     CDS             complement(11412..12110)
                     /locus_tag="U4A80_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1975197.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12130..12672)
                     /locus_tag="U4A80_001572"
     CDS             complement(12130..12672)
                     /locus_tag="U4A80_001572"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1975198.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12804..13808)
                     /locus_tag="U4A80_001573"
     CDS             complement(12804..13808)
                     /locus_tag="U4A80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1975199.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13805..14794)
                     /locus_tag="U4A80_001574"
     CDS             complement(13805..14794)
                     /locus_tag="U4A80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1975200.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14791..15597)
                     /locus_tag="U4A80_001575"
     CDS             complement(14791..15597)
                     /locus_tag="U4A80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975201.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15763..16719
                     /locus_tag="U4A80_001576"
     CDS             15763..16719
                     /locus_tag="U4A80_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1975202.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16796..17314)
                     /locus_tag="U4A80_001577"
     CDS             complement(16796..17314)
                     /locus_tag="U4A80_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1975203.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="U4A80_001578"
     CDS             complement(17402..17551)
                     /gene="rpmG"
                     /locus_tag="U4A80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1975204.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17779..18225
                     /locus_tag="U4A80_001579"
     CDS             17779..18225
                     /locus_tag="U4A80_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1975205.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="U4A80_001580"
     CDS             complement(18420..20315)
                     /gene="asnB"
                     /locus_tag="U4A80_001580"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1975206.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6144 a         4399 c         3554 g         6491 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     3481 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     3541 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     3601 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     3661 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     3721 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     3781 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     3841 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     3901 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     3961 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4021 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4081 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4141 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     4201 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     4261 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     4321 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     4381 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     4441 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     4501 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     4561 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     4621 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     4681 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     4741 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     4801 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     4861 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     4921 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     4981 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5041 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5101 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     5161 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     5221 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     5281 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     5341 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     5401 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     5461 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     5521 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     5581 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     5641 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     5701 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     5761 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     5821 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     5881 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     5941 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6001 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6061 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6121 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     6181 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     6241 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     6301 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     6361 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     6421 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     6481 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     6541 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     6601 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     6661 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     6721 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     6781 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     6841 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     6901 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     6961 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7021 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7081 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7141 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     7201 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     7261 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     7321 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     7381 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     7441 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     7501 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     7561 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     7621 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     7681 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     7741 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     7801 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     7861 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     7921 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     7981 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8041 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8101 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     8161 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     8221 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     8281 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     8341 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     8401 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     8461 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     8521 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     8581 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     8641 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     8701 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     8761 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     8821 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     8881 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     8941 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9001 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9061 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9121 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
     9181 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
     9241 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
     9301 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
     9361 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
     9421 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
     9481 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
     9541 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
     9601 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
     9661 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
     9721 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
     9781 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
     9841 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
     9901 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
     9961 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10021 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10081 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10141 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    10201 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    10261 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    10321 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    10381 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    10441 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    10501 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    10561 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    10621 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    10681 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    10741 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    10801 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    10861 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    10921 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    10981 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11041 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11101 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    11161 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    11221 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    11281 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    11341 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    11401 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    11461 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    11521 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    11581 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    11641 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    11701 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    11761 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    11821 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    11881 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    11941 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12001 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12061 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12121 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    12181 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    12241 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    12301 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    12361 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    12421 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    12481 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    12541 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    12601 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    12661 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    12721 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    12781 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    12841 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    12901 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    12961 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13021 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13081 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13141 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    13201 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    13261 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    13321 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    13381 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    13441 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    13501 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    13561 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    13621 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    13681 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    13741 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    13801 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    13861 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    13921 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    13981 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14041 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14101 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    14161 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    14221 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    14281 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    14341 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    14401 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    14461 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    14521 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    14581 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    14641 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    14701 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    14761 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    14821 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    14881 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    14941 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15001 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15061 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15121 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    15181 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    15241 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    15301 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    15361 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    15421 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    15481 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    15541 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    15601 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    15661 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    15721 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    15781 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    15841 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    15901 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    15961 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16021 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16081 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16141 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    16201 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    16261 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    16321 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    16381 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    16441 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    16501 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    16561 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    16621 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    16681 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    16741 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    16801 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    16861 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    16921 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    16981 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17041 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17101 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    17161 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    17221 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    17281 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    17341 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    17401 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    17461 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    17521 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    17581 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    17641 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    17701 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    17761 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    17821 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    17881 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    17941 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18001 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18061 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    18121 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    18181 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    18241 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    18301 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    18361 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    18421 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    18481 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    18541 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    18601 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    18661 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    18721 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    18781 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    18841 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    18901 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    18961 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19021 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19081 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    19141 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    19201 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    19261 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    19321 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    19381 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    19441 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    19501 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    19561 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    19621 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    19681 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    19741 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    19801 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    19861 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    19921 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    19981 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20041 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    20101 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    20161 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    20221 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    20281 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    20341 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    20401 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    20461 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    20521 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    20581 gatcgtgt
//
LOCUS       DASJRQ010000048        20586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJRQ010000048 DASJRQ010000000
VERSION     DASJRQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.048"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(941..1354)
                     /locus_tag="U4A80_001581"
     CDS             complement(941..1354)
                     /locus_tag="U4A80_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1975207.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="U4A80_001582"
     CDS             complement(1810..2295)
                     /locus_tag="U4A80_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975208.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="U4A80_001583"
     CDS             complement(2434..2649)
                     /locus_tag="U4A80_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1975209.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="U4A80_001584"
     CDS             2873..3673
                     /locus_tag="U4A80_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1975210.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="U4A80_001585"
     CDS             3692..5560
                     /locus_tag="U4A80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1975211.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="U4A80_001586"
     CDS             5553..6044
                     /locus_tag="U4A80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN1975212.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="U4A80_001587"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="U4A80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1975213.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="U4A80_001588"
     CDS             6604..7599
                     /locus_tag="U4A80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1975214.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7741..8991)
                     /locus_tag="U4A80_001589"
     CDS             complement(7741..8991)
                     /locus_tag="U4A80_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1975215.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
     gene            9401..9700
                     /locus_tag="U4A80_001590"
     CDS             9401..9700
                     /locus_tag="U4A80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975216.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            9773..11134
                     /locus_tag="U4A80_001591"
     CDS             9773..11134
                     /locus_tag="U4A80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1975217.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            11156..11416
                     /locus_tag="U4A80_001592"
     CDS             11156..11416
                     /locus_tag="U4A80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975218.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            11430..12182
                     /locus_tag="U4A80_001593"
     CDS             11430..12182
                     /locus_tag="U4A80_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1975219.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(12276..12881)
                     /locus_tag="U4A80_001594"
     CDS             complement(12276..12881)
                     /locus_tag="U4A80_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975220.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(12934..13908)
                     /locus_tag="U4A80_001595"
     CDS             complement(12934..13908)
                     /locus_tag="U4A80_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1975221.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(14286..15005)
                     /locus_tag="U4A80_001596"
     CDS             complement(14286..15005)
                     /locus_tag="U4A80_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1975222.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(15132..16403)
                     /gene="celB"
                     /locus_tag="U4A80_001597"
     CDS             complement(15132..16403)
                     /gene="celB"
                     /locus_tag="U4A80_001597"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1975223.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            16556..17953
                     /locus_tag="U4A80_001598"
     CDS             16556..17953
                     /locus_tag="U4A80_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1975224.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            18034..18474
                     /locus_tag="U4A80_001599"
     CDS             18034..18474
                     /locus_tag="U4A80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1975225.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            18622..18948
                     /locus_tag="U4A80_001600"
     CDS             18622..18948
                     /locus_tag="U4A80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1975226.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            18949..19698
                     /locus_tag="U4A80_001601"
     CDS             18949..19698
                     /locus_tag="U4A80_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975227.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(19778..20233)
                     /locus_tag="U4A80_001602"
     CDS             complement(19778..20233)
                     /locus_tag="U4A80_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1975228.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         6803 a         3483 c         4088 g         6212 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctgag tttagtctaa tggtgataag
     7621 tcaagatgaa atttaagatt ttctttattg agtaatatgt tttgaagcgc acgaaactag
     7681 acccgatcaa aatcaaattt ttttcgggtt gattcaagga tttgcgtcaa taacggattg
     7741 ttatttctct atcacactcc ttggtggcgt atagagttgg tgtttacgta gtagcgtatc
     7801 caccagacgc gtaaattttc ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg
     7861 aacctcctta tatttacttt gataaaaagc tttgtattca ggaagataat tttttacaga
     7921 gttggcagct tcaactaagt agtatctaaa atatcgattt ccttgttttg tcagaggtgt
     7981 attttgcgac tgatagtttc cagattggtt cactttccaa cttaatccag cgtattttgc
     8041 caattttgtc tgatcttcaa atctttcgat ttgaccaatt tcagcgatta aaccggcagc
     8101 atagacttta ccaacgcctg gaatactggt taagcattga tattctggaa tgacaacaat
     8161 tacttgttcg atggctttat ctaattcttt gatatttttt tcaagtgctc gtatttctcg
     8221 tacgagaaca cttaaaacaa cattaattga ttcttgtgca agagcaccta ggcggtaact
     8281 catggtaatt gcacgttgaa ttgctttagc gattttttct ggttgcttga aacggccttt
     8341 tcctttttct tgaagtaaat cacagaaagc ttctaaagga agctcagcaa gttcatccaa
     8401 tgtgaaatct tccgtcatga gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc
     8461 tcgcatttct cgagcaagcg tattacattt gtacgttaga ttttcaagaa aatgttgctt
     8521 tgtacgaatc aattgcttaa taagttgata gcgagtacgt gttaaacgct gtaaggccat
     8581 gtatttctct tctttgatag gggaagttgt gaatcgttga atacgcaaga aatcagcgat
     8641 tcttaatgca tcgtttcgat cagttttatt ctcatcaaac atgcgactaa attgtttcac
     8701 ccgaaaagga ttttcaatcg ttacaagtgt gttgagcttc tttagtttct catcttcatg
     8761 aaaaaacatg gaaggatgga agctgtacat ggacgttgac tccatgccaa tcacaatttg
     8821 atcaaagtgg tagctgttat tgaattcaag aattgtttct cgtatgaatg aagcaccttc
     8881 gatgtcattg gcaacagaga tttcagataa gatagacaat tgattgtcgt ctgttaaaaa
     8941 acaaacatct agtttttcag agctaacgtc aataccgaca aataatttca tggaagaagg
     9001 acctcctttc gatataaatt ttggaaaatg cttcgatact gtgggatttc ccagaaactc
     9061 actgtatagc cacaacctcg cctaatagaa tacggtgata aaagagctag aagctgcctt
     9121 ccaaactact atttgaaagt ctagtccatt atcgaaacag ctaatgagtt ggtagtgata
     9181 acagtggttc gggtaacaga ctaaacaggg tagacaaacg ctacaggagg aaatagcaaa
     9241 ttcccgttgg atcctttcca tacatccatt gtactctgag aaatcacaca atatcgaaac
     9301 aaattccgtt aggaatgtgt tattcaataa attttataat ctagaaacat tttgaataaa
     9361 aactattaga caaatgatat tttacgagga ggaagaaaaa atgaaaaatc ggttaattgt
     9421 cgcatgtgga agtggagtag caacgagtca gacaatcgct agtaagatcc aaagtatgtt
     9481 agaagatgac ggcatcgcat ttcctgtaga agcagttgat tacaaatcga ttcaaaatga
     9541 gcttctaact gctggaatct atgtgtatgt cgcacaacct gatgaggaag tacttgaaca
     9601 agcaaaagat ttgggtattg aagtatttcc aggaattcca tttttaactg gaatgggtgt
     9661 agaacctatt tatgacagta tcaaagagtt agtacagtaa aggaggattg ttggataaaa
     9721 aagttggttc atagttttat ttactatatt tagaaagaaa gaaggtttat ttatggaagt
     9781 tttccaaacc attatcaatt atgttttgaa cttaggatca gccatttttg ttcccctgat
     9841 tattttgata cttggattgt tggcagggat gaaattaaaa aaagcattca tgtctgcttt
     9901 gactttaggg attgctttta gcggaatgtc tatggtcatt ggctatatgt ctaacgcagt
     9961 aagtccagca tcagaagcat tggctaaaaa tacgggaatc agtttgcctg cacttgatct
    10021 tgggtggaca ggtgcagcaa gcatcacatg gtcgtggagt tatgctttta tattttttgc
    10081 agtaacgatt ggggtcaatt ttgtactgtt actttttaat tggacgaaga cgttaaatgt
    10141 tgatatgtgg aatgtttggg ggaaagcttt aactgcttat ttagtttatt atgtttccgg
    10201 tagtttggct gcaggctttc ttactgcaat ggtccaagtt atattggaat taaagcttgg
    10261 tgatatgttt caaaaacata tacaagatct cacagggatt ccattagtaa cagtgactca
    10321 ttttatgaca tctgccgctg tcttgttgct gcctttcaat atgatcatgg ataaaattcc
    10381 tgctttaaat aaacgagcgg atacgaatgc attgaagaag aaaattggga tcttctctga
    10441 aaactctgta atgggattca ttattggatt ggggcttggc tttgctgctg cgtatggcgt
    10501 ttcaggttct cttaatctag cgatccaaac agctacagca ttaagtttgt ttcctatgat
    10561 ctctaaaatg ttcatgcaat ctttgtctcc tctagcagac gctatgtcag aatttatgaa
    10621 aaagcggttt aaggatcgtg aagtatatat cggattggac tggccgatat tagctggtcg
    10681 ttcagaaatt tgggttactg ctattttact tgttcctgtg tttattggtt atgccattat
    10741 tttgccagga aaccaagtat tgccattagc agggattatc aactattcga ttgcggtcgg
    10801 cggattactt ttaacaggag gaaacttggc tcgtatgctg atcttgggaa tcatcactat
    10861 gcctatttat ttgtacgggg ccacttacct tgcgccaata ctcagtgatt tagcagaaaa
    10921 aagcggagca gtcgagggaa tcaagaaagg acagttgatc acatggtcat ccatagaagg
    10981 gccggagttc agggttttat ttgcggaggc atttaatagg aatattctcg ctattgtagg
    11041 atgcgtgatt tttctaggac tgtttgttct actttataaa tatatgataa aagcgaaagt
    11101 accgagtgaa cgttatgctg aactacaaaa atagcattga aatggtggga aagtaatgaa
    11161 aagagaagag acagataaaa taaaatggac agtcgctctt tgtggtacac ttctgctatt
    11221 tctatatgga ctctttactc agaatatcat catcaatcta ttggtcattt ttttcgcact
    11281 agtgatctat aaatatggaa atcacgtttt attcagagag tacgatgaaa aaagaaaacg
    11341 gaaaatagaa gaatcaatga aaataaaaga agctaccaaa gaaatattga gagaaaaaag
    11401 ttttataaaa agataatgga ggggtcatta tgtcagatgg acgtatgtta tttgagagag
    11461 aacgacagga catggcacgt atcgtaagag tgattttcga aaggaaaaac acaaatgtcg
    11521 caggagggaa tttttccttt aaaaccactg atgatcaagg gaaagactac atcatcatga
    11581 cgccgaccat gatgtcagaa gcctatctag gagagatttc tcctgcacag gttctagtag
    11641 tagaaccaag aacgcgtaga atcgttgacg gagtcggtca tttaactcgt gaaatcaata
    11701 tgcatgaagc gatgtatgag actaatccag ctatcaaagc tgtcctacat gctcatgcgc
    11761 ctaatgcaat gttctgggcg actactgggt tgcctatgcc taatttgaca gaagccacag
    11821 ctaaagtaaa agagattcct gtcatggact ttgagcctaa ttgctctgaa gaacttgctg
    11881 aattgacaag cgactatgta aaaaaacagc atctcgaatt accgcatatg ttgctgctca
    11941 actctcatgg cgtattgatc aacacaggtg gagaatcagg aatcgaagct atccataaag
    12001 cactgcaaat ccttgataca gttgaatgga atgcagaaat tgcctacaag cagacgattt
    12061 ttcaagcatt agggattact gacggttttt attcaaaagg tgagaaaatc gggggattgg
    12121 aagagttgaa acataaaacg gctatttata ataaatctgt tattgcagct ggtggagact
    12181 aacctatctg acacaaaaaa ctacgacttt cacaaaatct gatccagatc gatatctgct
    12241 tcaggttgta ttatgtaagt cgtagttctt ttcttttatt tagttaagag gtggtctgtc
    12301 cattccctcg cttgcttgat ttcgtccatc gtcaactgat gacctgttac agttagctga
    12361 atagtcacat ttgcttggcg tgtttcgaat tgatcggcca attctttaaa ggaagcaggg
    12421 gaaacgattg gatcgttatc accataagaa atccagacgg ttttatcctc taatgtggaa
    12481 cggctttgat gaacacctag agacatcgga tggaaaaaca accctttatt tagctcggtc
    12541 tcacgctcca ataataaatg agcagcgatg tttgcgccat ttgaatagcc gacaagcagc
    12601 cagtcactta acggtatcag ttctttttcg ctgatttctt tgattttttc atggagttta
    12661 tctgtttctt tttccaaact gtccaggtcg aattgattca atccattacg cttgaaaaaa
    12721 cgattcatcc catcttcttg gaccgtgcca cgaaaagaaa gcaatgtact gtttggtgca
    12781 agaaagtgag cgatatccag taaagaatgt tcatcgccac ctgtgccatg aagtaagatc
    12841 aaatgcttgc cacttgttcc tttttcaaaa atgtaggtca ttttgttccc tcttttctgt
    12901 ttctatagat gcaaatgaag tagcatgaag agattattca cgttttttat ttgcatcact
    12961 tgtatcgaaa ggacggacat aagcttcaat ggcttcacga cgttcttcta agaatggagg
    13021 aagagatagt ttttctcctg ctgattctgc tggttcatct tcaaggaatc caggaccatc
    13081 agttgctaat tcaaacagta cgtgatgagc ggctaggaag tactcagatt ggaaatagaa
    13141 acgatcgacg aaaccagaat ttggcaattc aatcttattg attacttgaa tccagtattc
    13201 caatgcttct ttgtctgcta cacgcaatgc taagtgatgg atatttccaa atccttcgat
    13261 tgcttttggc aagtctgtcc gatgttcaat aatgatgctt gcaccatttc ccccttcacc
    13321 gatttcaaat aagtagaaat ctcctgaatg gtctgtttct ttgaatccta agattttttc
    13381 aagaatcaag cggatatgct ctaattgact gacacggaca aagacaggtc caagtcctgt
    13441 gatcgcaaac tctgtcggta cattgctgtt tttccaagga atgccagctg ctacaccttg
    13501 attgttttcg tcagagatca attgataata ctgattatcg aaatcttcaa attcgatata
    13561 ttttttgccg aaacgttcat tgattgctga atgagtaacg tcatattttt caaaacgttc
    13621 caaccaaaag tctaatgctg cgtcacttgg cacacggaaa gaagcacgag agatcgtatc
    13681 tgttcctttt tttccttttg gctgatttgg aaaatcaaag aaagtcatat ctgttcctgg
    13741 cgcaccagta tcgtctgtaa agtaaagatg gtaagtttcg atgtcatctt ggttaactgt
    13801 ttttttgatt aagcgcattc ctaaaatatc tgtaaaaaag cggtagattt ttgtagcgct
    13861 agatgtcatt gctgtgacgt ggtgaattcc tctgatttgt gtatccatga taaatttcct
    13921 cctgttaact tacaaaaagt aagtagttga tttagacata gtatagaggg tgcatctcta
    13981 ttttgcaatt aatttgtctt ctaaagtatc aggaaggaat caagaatgga aaagaggagg
    14041 gacggatcaa taaaataaaa agacttacga ctagctgtat cgatttcaaa aaaactagct
    14101 ttaaaaagct aactttagaa tcattacggg tggtcataag tctttttatg ctgagttatt
    14161 ttttcatttt tgagctaatg cttaggatag aatagaatgt tgatttcacg atctcttctt
    14221 gttacttgaa actgcttacc actgtcacga tctcttggta aacggctttt tcacaacctc
    14281 tttggttaag ttgaacgcac atccagcaca gaatagttcc gaacatcaaa tcggtttcgg
    14341 ctggaagaat attcaatcgg ctgtccatct ttgagataga cgacttgctg aacttccagt
    14401 acaggatcgt tttctgcaca gttcaaatat tgctgatcat attcatcact tttatctgca
    14461 gaaatagcac gataagcccc tgcaaaatgg acattcaatt cttgatgaag atgatcatag
    14521 atagaggcta acaaatgttc tttttttaaa cccggcacaa gatggactgg cataaaggta
    14581 tgctctaaaa catatggctc gccatctaag atccttaacc ggtgaatatt gtagacaggt
    14641 tgctcggcat cgatcatcaa tcgttcttgg atatattgat tagggaattc aacatcaaaa
    14701 tgaatgattt gactcttcaa agaacgatgt ctgcgtttca tttggagact caaaccttca
    14761 tactcgttag cgggcgatgt gtctttttgc ataaatggat gattcaagat tttcgttcct
    14821 gatcctcgct gcgaatagac aagtccttcc attgctaaga tattgatggc ttttttgatt
    14881 gtcatccggc tcgcaccaaa ttcttttacc aagtcgactt gattaggcaa aaaactatcg
    14941 agaggatact cttttgaacg gatacgttct cttaaaatat ttgcgatttc ttcgtatttt
    15001 ggcacgttat tcacctcttc tagagtataa cgaaagatta tgcttccgac aactcgaagt
    15061 agcatctgga acattgttta ttcgaaaaaa gaggtcatga caagtttttg tcacaacccc
    15121 ttaatgatct attaagcaat atctaaatct ggttgtttat ctagtacttt caggaatggg
    15181 taccagataa aggcaacgat gacaaagtcg atgacttgca gcataccacc aagccatgaa
    15241 tttgttgcta agacaccgct tgcaatgatt ggcacagtcc atggaacaga gacgccagtt
    15301 ggtgcaggga caagtccagc agccattacc aaataattga acgtcgttac gattagaggt
    15361 gccacaaccc aaggaataag gattgttgca ttcaatacga tcggtagacc gaagatcgct
    15421 ggttcattta cattgaaaat accaggtgct aaagctaatc gtccaacatc acgatactgt
    15481 ttctttttaa gaacaaaggc catcaataaa acgacagcta aagtcattcc agagccacca
    15541 ataccaacag tgaaggtttc catgaatggt tttgtgatga tgtgtggtaa ttcttttcca
    15601 tgttgataag cttctaagtt atcaagcatc agtgtattcc aaatcgggtc cattacagag
    15661 ttgacgatga tttgtccgtg aagtccgaag aaccataaga attggacaaa gaacaaagca
    15721 atcaatgttg caggtaaact acttcccaaa ccagttaatg gtttttggat cacttcataa
    15781 acgacgtcat gtaagttagc attgaaaata ccagtcatca cagcattgat tactaagaaa
    15841 atagttagtg tcactacagc agggatcaaa gcggcaaatg attttgttac tgcaggcgga
    15901 actccatcag gcattttgat ctgccagccg cgtttcgtga ttctgcagta gatttctcca
    15961 gccaagaaag cagcgatcat tccaatgaac attccttttg cgccaagacg atcaagtgaa
    16021 agcacaccag tcacatcttc accgccagcg gttttcattg caaaaggcgt aagaatcaag
    16081 aagctggcaa aagacacggc accaccgaaa ataccttcaa cgtcataaga ttttgataaa
    16141 taatagccga taccaaaagt aacaaaaacc gacatgatgc tcattgttgc attttgccca
    16201 ttaccgaata aattcgataa agttcccttt gttgcgtcac tgaaaaaagg caagttattg
    16261 ataacgacaa cgatcgaacc gaacatcgtt aaagggaagg aaagcatgaa agcatcccgc
    16321 agcactgtaa ggtaacgatt ttgccctaat aaattagcta agggcatgat tttctcagaa
    16381 agcttgtcca taaaaccgtt catttttatt cacctcaaat ttatttgcaa tcgattacat
    16441 acaaagtata acgccaaaaa ttttaaatgt ctagtcattt tcataaaaag tattgattta
    16501 ttttttaggg ttcgttatga ttacggtatc aagaaaaaag agaggaagta aaaaaatgaa
    16561 aacctacaca ttcccagaaa atttttggtg gggttctgct gctagcggac cgcaaacaga
    16621 aggaagagta agtgatgatg gtaaaggaga aaatatttgg gatcattggt atgaattaga
    16681 gcctgaaaaa ttcttcgatc aagtaggacc tgaaaaaact tcgcaagttt acacaaaata
    16741 caaagaagat gttcagttaa tgaaagaaac tggccataac acatttcgta catctatcca
    16801 atggagtcat ttgattccag aagggacagg tgaaatcaat caaaaagctg tttcttttta
    16861 tcgagattat tttactgaat tgctggataa tggtattgaa ccttttgtga acctttacca
    16921 tttcgatatg ccactaccac tgcaagaaaa aggcggatgg ttatcaagag aaacggtcga
    16981 cgcttatgtt tcttatgcag cagcgtgctt tgaactgttt ggagatctgg tcaaaaaatg
    17041 gtttactcat aatgaaccaa tcgtacctgt agaaggaggc tacttgtatc aattccacta
    17101 tccaaatcat atcaatatga aagaagcggt ccaagtaggc tatcatgaga atctagcaag
    17161 cgcaaaggta atcaaagcct accatgaagg cggacatgat ggtgagatcg gaatcatctt
    17221 gaatctgaca ccaagctatc cgagagatga aacgaaccca gaagatgtaa aagcggcgaa
    17281 gatcgctgac gcattcttca accgatcatt ccttgaccct gcagtgaaag gagagtttcc
    17341 acaagagttg atcgaaattg tcaaagaatt ggctataatg ccagagatcc atgaaggaga
    17401 cctagaactc atccgtgaaa acacgattga cctgctagga atcaattact atcagcctcg
    17461 tcgaatcaaa gcaaaagaaa ctccaatcaa tcacgaacat agccccatgc cagatgatta
    17521 ttttgataat tacgacatgc ccaataaaaa aatgaatcca tatcgtggat gggaaattta
    17581 cgaaaaagga atctatgata ttttaacgaa tacaagagaa aactatggta atatcaaatg
    17641 ctatatttct gaaaatggga tgggggtcga aggagaagaa cgatttgtta acagcgaagg
    17701 aattattgaa gatgattacc ggatcgagtt tgttacggat catttgaaat acgtccatca
    17761 agcaatccaa gaaggaacca attgtgtggg ttaccacatg tggacatgta tggacaactg
    17821 gtcttggaca aatgcttata agaatcgcta tggctttatc gctgttgatt tggcacaaga
    17881 cggtaaacgg acgatcaaaa aatcaggtcg ctggtttaaa acggtctctg aaaaaaacgg
    17941 atttgatgcc tgatattttt tgataaaacg ttcacaaaat gtttgttcta acactgtaat
    18001 cgtgctacac taacagtgaa ggaggcgttt acaatggtag atacttatcg taatatttta
    18061 gtagcaattg atggttctga aaaggcagaa aaagcatttc ttgaggcaat cacgattgcc
    18121 aaacgaaatc aggcaacgtt gcacatcctg tatgtcaatg aagtaacagg caattacttt
    18181 ggtgattttg catttgttac aacaagcctt caggaagaat tggatgaggt tgcggaaaac
    18241 cagatgaaag aacatcgaaa cttggcaatt gaaaaaggcc tgacagatat cgagacatat
    18301 gttctttacg gttatccaaa gacactgatt gctaatttcc atgaatcgaa agaaccaatc
    18361 gatctgattg tcatgggggc gacaggtctg aatgcggtag aacgtgcact agtaggttcc
    18421 actacttcct atgtagtcaa ccacgcatca tgtaacgtca tggttgtaaa ataaataaat
    18481 taagggaaca gaaagcaaaa aagcataacg cttcttttgc tttctgttcc ttttattttt
    18541 ttgtaaaaaa ttactttacc tgatgaagta atcatgctat tctaattaca acgacagatg
    18601 gagtaatatg gaggaaagaa aatgatttca agtgatggaa tacgcggata caatgatgcg
    18661 attattttat cgatccttaa caaaaacgat tcttatggat atgaaatttc taaaacgatt
    18721 cgtgagagga caaatgattc ctatgtgatc aaagagacaa cactttactc cgtttttgcc
    18781 cgtttagaaa agaatcaact gattgtttcg tatcctggag aagtgactca tggaaaaaag
    18841 cggacatatt ataaaatcac tgaaataggg cgtaatcttt tagaagaaaa aaagcaagag
    18901 tggctgacga cgaaagacgt agtcgatcaa tttttggagg gaaattaaat ggatacaatc
    18961 cgtaattatt tagacagtct ttttatcgga gtacctcaaa gtaccgagat agataagctg
    19021 aaaactgatt tgttggcaaa tatggaagac cattatcatg aattgatggg agaaggaaaa
    19081 aatgaacagg aggccatcgg tacagtaatt tcgacatttg gttcaatcga tgaattatta
    19141 gaagaactag atgtcgaaaa aaaacatcaa gctgacgaga cggaaacaaa cacagcttct
    19201 atttatttaa gcgaagcaga aaactactgg aaagaataca gagcggcaag tctccaagtg
    19261 gcatcaggtg ttctattcat tagcttatct tttgcaagtt ttcttttctt ttgttcagca
    19321 ggatatgtgt tcatgggtat ttcttgtctg attttcggta tagcattagc tgtcggtttt
    19381 tttattgctt ctggtatgaa aatcacacgt ttaaatcact ttttgcacca tcggaaaata
    19441 cctgaaaaag tattagcaga agcaaaagaa aaagaagaag agtatcagcg aagttttggc
    19501 ttctcactga ttgcaggaat tggattatgt attttttcac ttttcccact tttggcttct
    19561 ttaatgtggt atatggatgg tagtatcggt gcctctatat tttttgttac ggtaggaacg
    19621 ggtgtcttct tgatcattta tggcagtcta gtccgtcatt cttatcgtca attcactcaa
    19681 agcgcttatt actggtgaat gtattcagat ttgtaaaaat attctattta tccggcgtag
    19741 aaaaaaagac tgtgacacgc ttttgtcaca gtctttttta tatataataa tttaaaaatt
    19801 catcatctaa atccgtagaa ttttctagat tgcttaccca tgtaaaatcg ttatagacag
    19861 atttcagttg acttctgcgc cattgtacag ggataaaaag acgcatgcta tcttcattga
    19921 atccgactaa taatttcttt ccgtcagtaa tgatgggacg ttgtaacaaa gtaaaatgcg
    19981 agacgatcag atcgacaagc tctgaggtcg tacattggtc gatttggtct ttcaaatttt
    20041 tataatcaat cgagcgttta gctaatagat cgtcaaatcc gttttctgtt aatgacaata
    20101 aataaaatat ctctttcctt gttaaaggct gtttaggtgt gaaaattcgt tctctgtaag
    20161 gcatttgatg gtctttcagc cagtttgcca tcagatggta actatgtgtg cgagaagaat
    20221 agatcgtaag cataaaaaaa ctccttaaat agtccattaa tttgttagat ctgttgagat
    20281 aaataatagg taaggagctg tgcataagca ttgatcaaaa caacccaaac atcattagaa
    20341 ataactttct aaagtcttaa acttgcgacg agaagcaact agactaatag aaaaaatgtc
    20401 ttagccattt ggcttcctgt ttgttttttt gcttatgctg cagctcctcg ggaaagggtt
    20461 ctaacaattt gaagaaggtt gatgattgat ctagtttaaa ttgtgtgaaa tttgaaatct
    20521 ggtgttttta ctagatgaat cacctaacaa ctatgattat aacttataga acttctgttt
    20581 tgcatt
//
LOCUS       DASJRQ010000049        20369 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJRQ010000049 DASJRQ010000000
VERSION     DASJRQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.049"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U4A80_001603"
     CDS             complement(<1..1835)
                     /locus_tag="U4A80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1975229.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U4A80_001604"
     CDS             complement(1832..2536)
                     /locus_tag="U4A80_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1975230.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U4A80_001605"
     CDS             complement(2548..4857)
                     /locus_tag="U4A80_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1975231.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U4A80_001606"
     CDS             complement(5052..5516)
                     /locus_tag="U4A80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975232.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U4A80_001607"
     CDS             complement(5516..6142)
                     /locus_tag="U4A80_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1975233.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U4A80_001608"
     CDS             complement(6149..6514)
                     /locus_tag="U4A80_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN1975234.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U4A80_001609"
     CDS             complement(6504..6842)
                     /locus_tag="U4A80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975235.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U4A80_001610"
     CDS             complement(6832..7158)
                     /locus_tag="U4A80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1975236.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="U4A80_001611"
     CDS             complement(7136..7417)
                     /locus_tag="U4A80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1975237.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="U4A80_001612"
     CDS             complement(7419..8783)
                     /locus_tag="U4A80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1975238.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U4A80_001613"
     CDS             complement(8796..9365)
                     /locus_tag="U4A80_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN1975239.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U4A80_001614"
     CDS             complement(9337..10524)
                     /locus_tag="U4A80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1975240.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U4A80_001615"
     CDS             complement(10588..12315)
                     /locus_tag="U4A80_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1975241.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U4A80_001616"
     CDS             complement(12312..12764)
                     /locus_tag="U4A80_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN1975242.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U4A80_001617"
     CDS             complement(12876..13256)
                     /locus_tag="U4A80_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1975243.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U4A80_001618"
     CDS             complement(13253..13639)
                     /locus_tag="U4A80_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975244.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U4A80_001619"
     CDS             complement(13641..13919)
                     /locus_tag="U4A80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975245.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="U4A80_001620"
     CDS             13967..14695
                     /locus_tag="U4A80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN1975246.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="U4A80_001621"
     CDS             complement(14841..15317)
                     /locus_tag="U4A80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN1975247.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="U4A80_001622"
     CDS             complement(15277..15624)
                     /locus_tag="U4A80_001622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975248.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="U4A80_001623"
     CDS             complement(15611..15796)
                     /locus_tag="U4A80_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975249.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="U4A80_001624"
     CDS             complement(15797..16069)
                     /locus_tag="U4A80_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN1975250.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="U4A80_001625"
     CDS             complement(16066..16269)
                     /locus_tag="U4A80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975251.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="U4A80_001626"
     CDS             16394..16606
                     /locus_tag="U4A80_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975252.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="U4A80_001627"
     CDS             complement(16639..16833)
                     /locus_tag="U4A80_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975253.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="U4A80_001628"
     CDS             complement(16846..17028)
                     /locus_tag="U4A80_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975254.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="U4A80_001629"
     CDS             complement(17025..17165)
                     /locus_tag="U4A80_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975255.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="U4A80_001630"
     CDS             complement(17655..18020)
                     /locus_tag="U4A80_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975256.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="U4A80_001631"
     CDS             complement(18060..18362)
                     /locus_tag="U4A80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1975257.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="U4A80_001632"
     CDS             complement(18362..18661)
                     /locus_tag="U4A80_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975258.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="U4A80_001633"
     CDS             complement(18654..18818)
                     /locus_tag="U4A80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975259.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="U4A80_001634"
     CDS             complement(18824..19093)
                     /locus_tag="U4A80_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975260.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="U4A80_001635"
     CDS             complement(19105..19854)
                     /locus_tag="U4A80_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975261.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..>20369)
                     /locus_tag="U4A80_001636"
     CDS             complement(19851..>20369)
                     /locus_tag="U4A80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975262.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
BASE COUNT         5644 a         4311 c         3175 g         7239 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtac
//
LOCUS       DASJRQ010000050        20361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJRQ010000050 DASJRQ010000000
VERSION     DASJRQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.050"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            292..1431
                     /locus_tag="U4A80_001637"
     CDS             292..1431
                     /locus_tag="U4A80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1975263.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="U4A80_001638"
     CDS             complement(1498..2073)
                     /locus_tag="U4A80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1975264.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="U4A80_001639"
     CDS             complement(2078..3052)
                     /locus_tag="U4A80_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1975265.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="U4A80_001640"
     CDS             3335..4303
                     /locus_tag="U4A80_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975266.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="U4A80_001641"
     CDS             4416..4694
                     /locus_tag="U4A80_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1975267.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="U4A80_001642"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="U4A80_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1975268.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="U4A80_001643"
     CDS             5151..5906
                     /locus_tag="U4A80_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975269.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="U4A80_001644"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="U4A80_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN1975270.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="U4A80_001645"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="U4A80_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN1975271.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="U4A80_001646"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="U4A80_001646"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1975272.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="U4A80_001647"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="U4A80_001647"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1975273.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="U4A80_001648"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="U4A80_001648"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1975274.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="U4A80_001649"
     CDS             10643..11185
                     /locus_tag="U4A80_001649"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1975275.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="U4A80_001650"
     CDS             11208..12764
                     /locus_tag="U4A80_001650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1975276.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="U4A80_001651"
     CDS             12778..13680
                     /locus_tag="U4A80_001651"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1975277.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="U4A80_001652"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="U4A80_001652"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1975278.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="U4A80_001653"
     CDS             15123..15545
                     /locus_tag="U4A80_001653"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1975279.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="U4A80_001654"
     CDS             16014..16244
                     /locus_tag="U4A80_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1975280.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="U4A80_001655"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="U4A80_001655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1975281.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="U4A80_001656"
     CDS             17655..17831
                     /locus_tag="U4A80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1975282.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="U4A80_001657"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="U4A80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1975283.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="U4A80_001658"
     CDS             18326..18709
                     /locus_tag="U4A80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975284.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="U4A80_001659"
     CDS             18878..19762
                     /locus_tag="U4A80_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1975285.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="U4A80_001660"
     CDS             19876..20112
                     /locus_tag="U4A80_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1975286.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="U4A80_001661"
     tRNA            20200..20280
                     /locus_tag="U4A80_001661"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="U4A80_001662"
     tRNA            20287..20358
                     /locus_tag="U4A80_001662"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta t
//
LOCUS       DASJRQ010000051        20329 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJRQ010000051 DASJRQ010000000
VERSION     DASJRQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.051"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..1765)
                     /locus_tag="U4A80_001663"
     CDS             complement(29..1765)
                     /locus_tag="U4A80_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1975287.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1762..2466)
                     /locus_tag="U4A80_001664"
     CDS             complement(1762..2466)
                     /locus_tag="U4A80_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1975288.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2597..3100
                     /locus_tag="U4A80_001665"
     CDS             2597..3100
                     /locus_tag="U4A80_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1975289.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3060..3398
                     /locus_tag="U4A80_001666"
     CDS             3060..3398
                     /locus_tag="U4A80_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1975290.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3419..4837
                     /gene="ffh"
                     /locus_tag="U4A80_001667"
     CDS             3419..4837
                     /gene="ffh"
                     /locus_tag="U4A80_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1975291.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            4949..5527
                     /locus_tag="U4A80_001668"
     CDS             4949..5527
                     /locus_tag="U4A80_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1975292.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5531..6052
                     /locus_tag="U4A80_001669"
     CDS             5531..6052
                     /locus_tag="U4A80_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1975293.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6131..6685)
                     /locus_tag="U4A80_001670"
     CDS             complement(6131..6685)
                     /locus_tag="U4A80_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1975294.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6938..7213
                     /gene="rpsP"
                     /locus_tag="U4A80_001671"
     CDS             6938..7213
                     /gene="rpsP"
                     /locus_tag="U4A80_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1975295.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7225..7476
                     /locus_tag="U4A80_001672"
     CDS             7225..7476
                     /locus_tag="U4A80_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1975296.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7601..8392
                     /locus_tag="U4A80_001673"
     CDS             7601..8392
                     /locus_tag="U4A80_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975297.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8562..9083
                     /gene="rimM"
                     /locus_tag="U4A80_001674"
     CDS             8562..9083
                     /gene="rimM"
                     /locus_tag="U4A80_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1975298.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9073..9834
                     /gene="trmD"
                     /locus_tag="U4A80_001675"
     CDS             9073..9834
                     /gene="trmD"
                     /locus_tag="U4A80_001675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1975299.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            9970..10317
                     /gene="rplS"
                     /locus_tag="U4A80_001676"
     CDS             9970..10317
                     /gene="rplS"
                     /locus_tag="U4A80_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1975300.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            10690..11352
                     /locus_tag="U4A80_001677"
     CDS             10690..11352
                     /locus_tag="U4A80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975301.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            11431..14658
                     /gene="fss3"
                     /locus_tag="U4A80_001678"
     CDS             11431..14658
                     /gene="fss3"
                     /locus_tag="U4A80_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1975302.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            14772..15086
                     /locus_tag="U4A80_001679"
     CDS             14772..15086
                     /locus_tag="U4A80_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1975303.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            15099..15473
                     /locus_tag="U4A80_001680"
     CDS             15099..15473
                     /locus_tag="U4A80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1975304.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            15483..15827
                     /locus_tag="U4A80_001681"
     CDS             15483..15827
                     /locus_tag="U4A80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1975305.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            15898..17250
                     /locus_tag="U4A80_001682"
     CDS             15898..17250
                     /locus_tag="U4A80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1975306.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            17310..18452
                     /locus_tag="U4A80_001683"
     CDS             17310..18452
                     /locus_tag="U4A80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN1975307.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            18477..18731
                     /locus_tag="U4A80_001684"
     CDS             18477..18731
                     /locus_tag="U4A80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975308.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            18987..20171
                     /locus_tag="U4A80_001685"
     CDS             18987..20171
                     /locus_tag="U4A80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1975309.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            20168..20305
                     /locus_tag="U4A80_001686"
     CDS             20168..20305
                     /locus_tag="U4A80_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1975310.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         6758 a         3617 c         4403 g         5551 t
ORIGIN      
        1 tttaaaacgc acgagcagtt gctctttttt acattttcgg aatggtaacg gtgaaagtcg
       61 aacctgttcc aagatgactg tctacagtaa tcgtaccatt aaggagttgc acgtagtctt
      121 ttacaatcgc taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc
      181 ggtaaaaacg ctcaaagatc cgttcctgat cctcaatgtc gatacctata ccaaagtctt
      241 ggacagaaaa cgatagatca tctgttgcct gatagcggat aataatctcg ctgtcgcttt
      301 ttgaatattg gacagcattt tcaattagat ttttgataat tggataaagg atatctgttt
      361 tagtagtaaa tggttcgttc tttccaatca atcgaatcgt caaccgtttc ttttcgatga
      421 ttggctgata attttgaacg atttgctgga aataaggttc tgtataaatt gtctggatct
      481 cgtaagaatt ctcgccatct tttgacagct ggatgatttc ccgaatcagt ttatctaacc
      541 ggatcgcatc tttttgcatg atctcaagaa aagaagtcag agtttgcggg tcatcttttg
      601 caccatccag taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt
      661 gagagacgtt gccaacgaaa tccttttgta gcttttctaa acgtcgcact ttcgttaagt
      721 catacgccac ccctagaatc tgtccggtac cttctgaatt agtaaagtag cgcaaactca
      781 agtctaatgt ctgccgacct tctgtaaggg tgatctcctg atggatcagc ggatgctcag
      841 gagtcacttg atgaatcagc tgaatgagtt ttggctcttg aatgacttct gtgtaccgct
      901 gttcaggttg atagcttacc acgtttaaat gtagctgcat ctttggatta agcagtagta
      961 aacgagaatc agcatcatca atcaggaata cgccaatcat cagttcgttc agtaacgtat
     1021 ataattgctc ttctgtggta gtacacgccc gatacatctt actcatttgt tcgctcaacg
     1081 cattaatcgt ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt acttccgcct
     1141 gattaggatt ttgcaccatt ttttttagta cgggcagcac ggttttcaat ggctggtttt
     1201 tttgatagat cagataatag atcattcctg caagaacaag gaagaaagac agaaagaaga
     1261 aaaagaccca acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga
     1321 tacgtaaaat ggcttctaac tggccacttt ttttgactgg taatgcgaca tataaaagat
     1381 cctcattcaa agtagtactt ttgcgcaaag cgctacctag gttcccacca ttcaatacgg
     1441 ctttgatttc gggacgggag tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata
     1501 agatctttcc agtagcatcc attaaagtaa tccgttcgtt cgactgatgg acgaattctt
     1561 ccaaagcagt tttgttttgc gctgcttgga ggttttcgac atctaattgc cggatcagca
     1621 gttgtgcttt ttttgtcagg tagctttctt gctgttccac gatctgctgt tgaaaataat
     1681 gggaaatcaa ttgccagcca ccaaaaaaca aactaactaa aagaataaga aagaagagac
     1741 gttcataaat ttttttccat ttcatcgttt gggttcctga aacttatagc caaatcctct
     1801 aatagtcacc aagtactttg ggtgttttgg atctcgctcg attttttctc gcaaatgact
     1861 aacgtgtaca tctacgattc ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag
     1921 caatgtgtct cggtcgatca cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa
     1981 ttctttcggt gttagttcaa tcagatggtc gtctacggtc acttgataat tggtcaaatc
     2041 agcagtgatt tgtccaatcg atagatattc aggctcagct tcatcttttg attgcttacg
     2101 aggttcaatt cgtctgaata ttgctttcat acgggctaat acttcgcgag gactaaatgg
     2161 tttggtcaaa tagtcgtcag cacctatttc taggccaatg atccgatcca cttggtcatc
     2221 tttagccgtc agcatcaaaa tcggtgtttc atttttttct tgacgcagcc gctgagtgat
     2281 ctccatgcca tccatcgttg gaagcatcac atctaaaatg atgaaatcaa atgattgttc
     2341 taatgctaat tctaatcctt cgcctccatc tgtggcactg gttaccttgt agccttcttt
     2401 ttccaaattg aacgtcaaga gcgttacaat agaaggttca tcatcaacaa ctagtatttt
     2461 tttcattcaa atctccttta aataggtttc attctgttta tattttagca tagaatcagt
     2521 acaaagggct attttacaaa agaaataaat ggtataatag gaaggcactt tgctgaatag
     2581 aaaggaagag cagtcaatga ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta
     2641 tgatggacag aaaaaagaag taccagaatt gatggaacta gttgaaactg gagaagctgt
     2701 catggtctgt ccagaagtga tcggcggatt accaattcct agagatcccg ctgaaatcat
     2761 tggtggaaat ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga caagcggcga
     2821 agatgtaacg gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa
     2881 acacattgat ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc attcgattta
     2941 tgacggaagt ttttccggaa taaaaaaaaa tggtgccgga gtggctactg cttattttat
     3001 tcagcaaaaa atgactgtgc tctctgatga agaatggctg aaaactagag gtgagcagaa
     3061 tggaaatcga aaagaccaat cgaatgaacg cgctctttga attttattcg accttattga
     3121 cagaaaaaca aatgaactac atggaattat attatgcaga tgatttttca ttaggtgaga
     3181 tcgcggaaga atacgaagta agccgacaag cagtatatga caatatcaag cggacaagca
     3241 agatcttgga ggattatgaa aaaaaactcc atcttttttc taattatatt gttcgagaac
     3301 aggtattgga aaatatgact gcatatatta cagataagta cccggaagat aaaaaactac
     3361 ttgaatatgt acagcaaatt caagcaatcg aagaataaat aaaaggaatg aagaaaatat
     3421 ggcatttgaa agcttaactg aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg
     3481 aaaagtatca gaagctgacg taaaagaaat gatgcgggaa atccgccttg ccttactaga
     3541 agccgatgtt aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt
     3601 aggggcagaa gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga
     3661 agaattgacg atcacacttg gttctgaaac agcagagtta aataaatcac caaaaatacc
     3721 aactgttatc atgatggctg gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt
     3781 agccaattat ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata
     3841 tcgaccagct gcgatcgacc aattgaaagt attaggtcaa cagctagacg ttcctgtatt
     3901 tgatatggga actgaagtca gcccggtaga gatcgtccgt caagggatgg agttagcaaa
     3961 agaaaagaaa aacgactatg tattgatcga tacggcagga cgtctgcata tcgatgaaac
     4021 attgatggat gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt
     4081 tgtcgatgcc atgactggtc aagatgccgt caacgtagct gatagcttca accagcaatt
     4141 aggtattaca ggggtcgtca tcactaaact tgatggggat actcgaggcg gggcagcact
     4201 atcgatccgc tcagtgacag gcgcaccaat caaattcatc ggttctggtg aaaaattgac
     4261 tgacttagaa gtattccacc cagatcgtat ggctagccgt atccttggta tgggggatat
     4321 gctgacattg attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc
     4381 acagaaaatg cgggaaaatt cattcgattt caatgacttt attgaacagc ttgatcaagt
     4441 gatgggtatg ggcccactgg aagatctaat caaaatgatc ccaggaatga atcaagtccc
     4501 tggaatcgaa aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag cgatggttta
     4561 ttctatgacg ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg
     4621 aattgctgcc ggttctggaa acagtgttgt tgaagtgaat cgaatgatca aacaattcaa
     4681 ggaatcaaga aaaatgatgc agcaaatgtc caaaggggat atgaacatcc caggtatgga
     4741 ccaaatgttt ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga atcgaatgat
     4801 caagaaaaat aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac
     4861 agactgaaaa tgtctactgc cggagctttt ctatcagcaa tttttattct ttactaatac
     4921 aattactcta aacgaagagg tggcacagat gagatgtccg tggagtgaac aaacagaaag
     4981 tatgcggcaa tatcatgata ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt
     5041 ccgaaaattg atgcttgata ttaatcaagc cggattgagc tggcagacga ttttaaataa
     5101 aagtgaagca ttcgatgaag catatgatcg attcgatatc aaaaaagtcg caaattatgg
     5161 agaaacaaaa atcagtgaac taatggaaaa caaaggaatt attcgtaacc gaagaaaaat
     5221 cgaagcagcc gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt
     5281 ttcagagtat ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt atcattcaca
     5341 agaagaagtc ccaacaacaa gtcctctttc agatgaatta gcaaaagatc taaagaaaag
     5401 aggatttaaa tttatcggaa gcacaacaat ctatgctttt ttagaagctg tcgggatcat
     5461 caatgatcac cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg
     5521 cgaataaagc atgcgaaaaa tcatatttta tggtgctata agtttagatg gctatttggc
     5581 aacgaaaacg gatgatcttc aatggctttt tgacactcca acaggtaagc aaactactta
     5641 tgaagctttt tatgaaacaa tcgatacgac catcatgggc agaaaaactt atcaggaagc
     5701 aaaaaaatat ttggagaccg aagcaattta tccagagaaa aagaatttcg ttttttccac
     5761 ccacgcacaa ttacaattgc cagatgctga ggtcgtaaac gaagatcctg ttgcttttct
     5821 aaaaaatcta caaatgacag aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat
     5881 caaaccgcta ttggaacatc aaatgattga tgaatggtat atccagatta cccctgtatt
     5941 attaggggac gggattcctt tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct
     6001 agacacgaca cgattcggtg aatttatcga attgcattat gtacgaaagt agaagtaaat
     6061 aaaataaaaa aggcaactat atgcagaaat gtcctgtaca tagttgcctt ttttattacc
     6121 gttatgcttt ttactctgtt aatttgttag aaaaatctag atacgcatct aaaatcgttt
     6181 ggaagaaact taatgttcct tcaacgatat taccattttc atcaattaga ttcgcaacgt
     6241 ttccgatata tgcttccggt tgctgcaagg ttggcacatt caaaaagaca agtgattggc
     6301 gtagatggtg gtttgcgcca aagccgccga tcccacctgg tgatgcagta acgactaaac
     6361 ctggtttatg atcccaaaca ctgtgtccat aaggtcttga gcctacatct aaagcatttt
     6421 tcaatactgc tggcacagaa cggttgtatt caggagtaac gaaaacgaca ccgtctagtc
     6481 ccctcacttc ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga gtttcgagat
     6541 cctcattgta aaatggcaga tcatcgattt ttacaaatac actttcgtac ccttcaggaa
     6601 gcaatttcgc aaaagcttct gctacttttt tgttgtaaga atcttttctt aaactaccaa
     6661 taaaaaatcc aatttttttt gtcatacgat ttttcctcct tcaaactatc tataataatc
     6721 ttacaaaaga taagtaactg gagcaaataa aaacacttac tgacaaaagc tacatagaag
     6781 atagattact tatttttaac aaaaaagaag tctaaaatct tctattttct aaaaaaaatc
     6841 gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta
     6901 catcttgtga ataaaacaat ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa
     6961 acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc
     7021 tcgtgatgga cgtttcatcg aaactgtagg tacttacaat cctttgaaag accctgcaga
     7081 agtagtttta aaagaagact tagttcttga ctggttatca aaaggtgcac aaccttctga
     7141 cactgtacgt aacatccttt caaaagaagg cgttatgcaa aaacaccacg aagctaaatt
     7201 ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg acttagtctt aacaatcgtt
     7261 cgtccgctag tcacttatcc tgatcaagtc aagttagacg tcgtagagtc gaaagacttt
     7321 tacgagtaca atctaacagt ggctcctgaa gatattggcc gtatcattgg aaaacaaggg
     7381 cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa
     7441 gtgcgtttga atatcatcga tgataaggaa aagtaaataa acgtgctgta ctcgaatttt
     7501 cgagtgcagc ttttttgttt tttttattaa ttgaagaaat gattatttgg caaaacaagc
     7561 aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt
     7621 tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac
     7681 acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac
     7741 ggcttccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga
     7801 ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg
     7861 ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac
     7921 agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta
     7981 tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc agccatttga
     8041 gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg
     8101 tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg
     8161 gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga
     8221 agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac
     8281 gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca
     8341 tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg
     8401 cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc
     8461 gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac
     8521 atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg
     8581 taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg
     8641 atttcccaga agaacgttac aaaaaaggaa cggtgctaac actattccaa gacggaaaag
     8701 cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt
     8761 ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt
     8821 caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc
     8881 tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg
     8941 ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg
     9001 cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag
     9061 gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct
     9121 atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct
     9181 aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc atacggcggt
     9241 ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa
     9301 gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat
     9361 cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat
     9421 gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat
     9481 gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg
     9541 ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg
     9601 gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg
     9661 acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc
     9721 tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt
     9781 ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt
     9841 ctaaaatctt ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc
     9901 acttaactat tacgatattc cgctgtgaga aaagctcata agaatatttg gaataaggag
     9961 aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc
    10021 ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt
    10081 gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa
    10141 acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat
    10201 acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg
    10261 tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatcag aagataaaca
    10321 cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc ttgtatatca agggcttttt
    10381 cttttttctc tatcacagtt ttaagccatt tttggctgtt ttggaccatt tttcaatcat
    10441 attcccttaa aaattgtcct agattgtccg acattgacta tcagtgtcgt caaaataggg
    10501 caaaatttag tagtcagctt gacgacaaat ataatacgaa aaataccaaa acatttaccg
    10561 aatttagcaa cttttgaacc caagtttctc aaagggtcag ctattagcaa aaaagacaaa
    10621 acatgtactt ttctactaaa aagttgatgt gataggcttc tatctaaata actattagga
    10681 gtttttatcg tgaagcaaaa tgatggacgt atcatttgga aaaatcaaag tgagttaaaa
    10741 ctgattttaa caattaatga attcatcgaa aaacacggaa ttacatctag ccgtcaatac
    10801 cagaaaaagc tatcagaaaa tccaaattct gcaccaagta tgtggttcat taacaaaaaa
    10861 tatggatcat gggagaattt attgattagt ataggacgag aaaatactgg gtatggaaaa
    10921 tgggctagaa tgtcagaaca agaattattg gaaatagtag aagcctttat taagtgcgag
    10981 aagataacct ctcaacgaat gtacgaacaa aaatcagtag ggaagaatat tccttcactt
    11041 agtaccatta aaaagatgtt aggagatata cgtccacttt ttaaagaaaa aaatgatgga
    11101 tctcgattta ctgatttcga actgctgtta gaactaaaaa atgaaataat ccgattaaaa
    11161 ctacaggatg atttatcaat gacaaagttt cgaaaactag ttcaatcccc aaaactacca
    11221 tcagttgata caattatgaa aagaacgaac aaaaattggg aggaattgat ggcagagatt
    11281 ggatttgatt atcgaagaat caaaatttat aagcagagaa acaacctatc caagacaaag
    11341 aaaactaaat agtatcactc taaatcagac gttcaaaatt aaaatttgga cgtctttttt
    11401 attacccaaa aacgaaagga gatttatttt atgaaaaagc caagatttaa gaattggcgt
    11461 ttgctcgcaa cactgagtct attatgccaa acaattggtg gatcgcttgg cccaacgatt
    11521 gcctttgctg atgaaattac tcatccacaa acggtaactg tggaattgga tttggctcac
    11581 cagtatgctg tcgaaggtac atttagcgat ggtcgtccca tgtcagaagt taccgttccg
    11641 cactatgcag tttacaatgg tgtgaagcaa gatattttct gtattgagcc aggggtgccg
    11701 atttacaatg agttcactcc tggttatgag aaaaatccat tgcctgatat gtcggagaaa
    11761 gcgaaattag tttccgtttt atggaaggaa gctggtacgg atgtggatac acatattgtg
    11821 gcccagaaga tgatttggca agaagttaat ggctacacgc ttcactcaat taaacgttct
    11881 gatggtagtg cagtaaatat cgcagccatt gagtcaaaaa tcaatcaagc cattgcggat
    11941 tatcaaaaga aaccaagctt ccataactcc acagtgaaaa ccgtgttagg tcaatcgact
    12001 actgtgacag atacgaacgg cttgaattta tcggagtttg atgaagttgt cgagaatatc
    12061 gcaaatattg attatcgtgt gaacggcaat caactagtga ttactccaaa tgccaattca
    12121 aaagagagtg gtgaattaac cctaaagaag tcagctaata caggaacacc ggtagcttat
    12181 aaaatggctg gtcaacaaac tttgatggcg ggggcaattg ataagcccaa tacctacaca
    12241 atcaaaattg atgtggaaac tgagggttct ttgaagatta aaaaagtcga taaagaaaca
    12301 ggaaatgttg taccgggaac cgttttccat ttagactttg gaaaaacatt acctgcaaaa
    12361 gacgtgacca ccgataaaga aggcattgcg acattggatg agattcctca tggcgcgaaa
    12421 gtaaccatta ctgaaaaatc agtgccagct ccttatacga ttgacactac accaatcacc
    12481 gcaaccatta aatctggtga gactatttct gtgacttcaa aaaatactcg cgaaaaaggt
    12541 caaatcattt tgaataaaat gggggtggaa acaggaactg atctttggaa cgacaaatac
    12601 tcattagccg gaaatacgtt tgccattcgc aaagacagtc caaccggtga aattgtccaa
    12661 gaaatgacca cggatgaaaa aggtcatgcg gaaacaccaa aagagattgc caatgcgttg
    12721 gaattgggaa cctattatgt gaccgaaacc aaagctagta atggctttgt gaataccttt
    12781 aaaccagtaa aagtcgaatt gaactatgtc aatcaaaccg tggctcttgt taccagtaac
    12841 gtaaaagggc aaaaccaaga aatcactggt gaaaccactt tgacaaaaga agacaaagat
    12901 accggtaatg aaactcaagg taaagctgag tttaaaggag ctgaatatac tctctttact
    12961 gcaaaagatg gtcaagcggt caaatggagt gaggctttta aaccagagtt agtgaaggga
    13021 acgaaagctt ctgatgaaac ggtgactttg actttagatg aaaagaatca agttgctgtt
    13081 aaacacttag cgattaatga gtattactgg caagaaacca aagcacctga aggatatacc
    13141 ttggatgaaa caaagtaccc tgtttccatc aaaaaggtgg atgataacga aaaaaatgcc
    13201 gtgattaccc gagatgttac ggcaaaagaa caagtcattc gctttggctt tgatttcttt
    13261 aaatttgctg gatcagctgc tgggactgcc gaaactggct tcaatgatct gacctttaaa
    13321 gtatcgccat tggaaggaac cagtgagatt acaggtgccg aagatgaagc aattacagct
    13381 tataacgagc aactaggttt tgatggctat ggcaagtttg aaaatcttcc ttatggggat
    13441 tatttacttg aagaagtaga ggctccagaa ggatttcaaa aaattacgcc attagaaatc
    13501 cgttctactt ttaaagaaaa taaagcggat tacactaaga gtgaatacat ttttacgatt
    13561 accgaaaaag gacaaaaaca accaattaaa acggtgaccg ttccttacga gaaactgacg
    13621 aataaagcat tttctgttag cttgaatcgt ttgatgctct atgatttacc tgaagaagaa
    13681 gatagcttaa cttctcttgg gacttggaaa gacggaggca aagaattgac agaccttgat
    13741 tctaccgagc tagttgataa attgagctat aacttgcatg aaatcaaaga agactggtat
    13801 gtggtagcgc aagccattga tgtggaagcg acaaaagctg cccaagaaaa agatgaaaaa
    13861 gccaaaccag tggtaattgc cgaaacaacc gcaacgttgg cgaataaaga gaaaacagga
    13921 acttggaaaa ttttgcataa attaaccgca gaacaagttt tggataaaac catcgtctta
    13981 ttcaactatg tgtatgaaaa caagaaaccc tatgaagcag gtgatgatcc agtagcgaaa
    14041 gatgctagct tgaacaatca agcccaaact gtcaattgta cggtggaacg ccatgtttcc
    14101 atccaaacaa aagcccacct agaagatggt tcgcaaacct ttactcatgg ggatgtggtg
    14161 gatatgttcg atgatgtgtc gattacccat gatgtactgg atggctcaaa agaagctttt
    14221 gaaacaattc tgtatgcatt acttccggat aatacgacca aagaaatttg gaaatctggc
    14281 aaaattgatt atgaagtgaa tgacaaagaa ttcactaaaa ccgtacttgc ggaaaaagtg
    14341 gacacaagta aatatccaga aggtacaagt tttaccttca aagaaattaa ctatggcaaa
    14401 gacggaaaca tcaatgggaa acacaatgag gatctaaaag aaaaaactca aactttaaca
    14461 cctaaaaaag ttccgacaac accaagtgca ccagaacaac cggaaatacc aactgttcca
    14521 agtgactctc aagaatctag tcctacagta aagacattcc cacaaacagg ggagaaaaat
    14581 tctaatgttc tgctgtttat tgggtttacc ttgatctttg cgactgctgg gtattatttc
    14641 tggaatcgcc gaaactaagg tgatgtgatg aaacaactaa ataggaaaaa ccaacgtgcg
    14701 cgaccgcctc ctaaaaaaag tggtcgcgct atttttgtat ccaaaactaa aaatgaaaag
    14761 aggaaaagaa gatggaattg aaatttgttg tgccagatat ggccgaaacc tttggaaaga
    14821 taaggtatgc cggcgaaggc gaagttttaa ctgaaggata tgggcggaat actacagtaa
    14881 ttggtcgtag ttatcatttg tattccagta agcaacgagc cgatgatatt gaagtggtgg
    14941 tagctgcgga agccggcgaa aaggattttg atcaagatca accactaaaa gccgtgaatc
    15001 cccatttggt tgccaaaggc tatgagattg agaatcgtgg gtttaccgac tacgtgttgt
    15061 atgtcgatga tttagtgaaa gcataggagg aaaagaagat gagattaaca gaaggtattg
    15121 ttgtagattc aggactaacg tttgggaaat tacgattttc cgcattacgc cgagaagtac
    15181 ggaagcaaaa tgaggatgga acggttagta acgaggtaaa agaacggact tataatttga
    15241 aatcctctgc gcaaggtcga atgatccaag ttagtatccc agcaaatgta cccttacgcg
    15301 agtttgccta tgacgcagag gtagagttgg tcaatccaat catggatacg gtcgccaatt
    15361 atgttttccg agaaggaact actgtcaatt ggttcattaa ggccgatgat ttagtcttga
    15421 aacgacaacc agatcaggga aattctacaa accaaaatga aggaaagaaa taggtgaagc
    15481 gtatggaaat taatgttgaa catattttag attgtctcga tcaatatgga aaaggtgagc
    15541 taacggagga acaactcact aaagcattaa cctatgatga aaaaatgttt ctgattatgc
    15601 atcaagggtt actggaagta ggtaacgatg tcgaagaaga ttttgatgta ctgaattggt
    15661 taggggaaga agagtccttc tttatggtta tcgaagtaaa tgaaagtcta tgtcgagaag
    15721 ccgaaaccgt attggaagaa attggtgtgg aaatgccgga tgccattgaa gtttttttaa
    15781 agcaactagt ggaaacgaaa caactcccta tagcagtaaa tgactagcat aactgcttga
    15841 aattggatca atgaaatcct atgaagcttt tttcgattgg aggtggaaac cataaaaatg
    15901 tacaaagggc atcgtatacg ggcaggagat cagcacttgg tttatcactt tgttcttggc
    15961 tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga gcttagacaa
    16021 tttgatattt ctaagctatc actttctact atagaaatcg ttcgttctat aaaagattta
    16081 atctatttgc taggaagtct cgttctttca ggaagcacga tgttgcttta tatacacttt
    16141 tttcaggatc attggcgcag tttgtggcat cgacaaaagc tagcccggat gattctagaa
    16201 aatcattggt acgaagtaaa acaaactcag agtgaaggat ttttcaaaga cctcaatagt
    16261 agtcgaacca aagagacaat cagttacttc cccaaaatct attaccgtat gaaggatggt
    16321 ttactttcta ttcgcgttca aatttcactg ggaaaatatc aagatcagct tttaaagctg
    16381 gaaaagaagt tagaaagtgg tctatattgt gaactagtag aaaaggaact caaagactcc
    16441 tatgtggagt acactttact gtatgatata attgccaatc gaattgggat agacgaagta
    16501 gtggcagaaa gtggggcttt gcgattgatg aaaaatcaag tatgggctta tgattcttta
    16561 cctcatatgt taatcgctgg cggcacaggt ggtgggaaga cttacttttt actcactatc
    16621 atcgaggctt tattaaaatc tgatgccgag ctttttgttc ttgatcccaa aaatgcggac
    16681 ttagccgatt taggtacggt aatgcctcat gtctattccc aaaaggagga aatttctgct
    16741 tgtgtggaag atttctatga gcgcatgatg actcgtagca aggcaatgaa agaaatgccc
    16801 aactacaaaa caggagagaa ttatgcgttt cttggacttc caccaaactt tttaatcttt
    16861 gatgaatacg tggtttatat ggaaatgtta acgacgaagg aaagtgcggt gattttgaat
    16921 aagctcaaac aaatcgtgat gttaggtcgt caatctggtt tctttctgat tctagcttgt
    16981 caaagaccgg atgcgaaata tttaggagac gggattcgcg atcaatttaa tttccgggtg
    17041 gctttgggtc gtatgagtga actgggatat tccatgatgt ttggcgaggt tgataaaaaa
    17101 ttctttatga agcgtatcaa aggtcgaggc tatgtagata cgggagggag tgtgattagt
    17161 gagttttata cgccacttgt accaaaagga tatgatttct taagggaaat tagcgacatt
    17221 ggtggattaa acgttaatac ggaaggataa aataatagta tgtagcctag gagtgtcaag
    17281 aatcctgggc tttttttacg aggtgattta tgacattttt agatttattc gctgggattg
    17341 gtggcttttg cttaggtatg gaacaagcgg gtcaccagtg tatcggcttt tgtgaaattg
    17401 atgattttgc tcgccagagt tataaggcaa tccatgatac aagtaaggag gtggaaatgc
    17461 atgacatcac aagtgtatca gacgagttta ttcaatctct cggaccagtg gatatccttt
    17521 gcggtggatt tccgtgccaa gctttttcaa ttgcgggaaa gcggcaagga ttttccgata
    17581 ctcgaggtac tttattcttt gaaatcgctc ggttcgccgc tattctccaa cctaagtttt
    17641 tattccttga gaacgtccgg ggattactca atcacgaagg aggggctacg ttcgagacga
    17701 tcctccgaac gctggatgga ttggggtatg atgtggaatg gcaagtgctt aactcaaagg
    17761 cctacgttcc ccaaaaccgt gagcggatct tccttatcgg acattctcga gacgcatgta
    17821 ccgaacaagt atttcctatc attggatcgt ctccaacatc tgatcaaaac atcagaaact
    17881 tattaaatat caacccttcc aatcgaggaa tggggggaca agtctatggg tcagatgacg
    17941 tagcgcctac cttgaccggt gatgaaggaa tcaaaatcgc attgccggta gatgatggaa
    18001 tttctgtagc gggtatgtta ctgggaaatt ttgaacaagg caatcgggtt tatgagataa
    18061 ctggaacagc gcctactttg tcaacgaaac aaggcggaac gaagatcatg cttcggaaaa
    18121 acgaaacaga atatcaagag gtgaagccag ggaacagtgt gaacctggct tttcctaatt
    18181 ccacaagtcg tcgaggccgt ctgggaaaac aaagtgtgca taccttatta acaggagacc
    18241 aacaagcagt cgtcacggat cagtatcaaa ttcgaaagtt aacacctagg gagtgctggc
    18301 ggcttcaggg ctttccggac tgggcttttg atcgagcttc tcaagtcaat tcagatagtc
    18361 agttgtataa gcaggctgga aattctgtaa cagttccggt catttttgat attgctagaa
    18421 gattaaagga ggtaaaaaca gatgatttat gatgagttga ttggggaatc tactgggtga
    18481 ttggcaagat tcagtcagat ccggaattgg aagaagaact gtaccggctc aattttgata
    18541 ttcgcaaaaa cggggtgaaa gtccctgatg atccgtatgt aattgatgag gaaacggatg
    18601 ctcgaatgga gctgaatcaa gtcatcagtg aattggaacg aattgcggac tttgctaaag
    18661 aacctgatat ccgccaatac ttgtttgaga taaaagcaga gttggaaatt gagggtatta
    18721 ccgcggagta acggccaaat aaaccactga taggcactga ccgcggggga tttgcgaagc
    18781 agaaatcctc cgtggtgctg tgcctgttta tggacggcgt tagccggcca tgatgtctca
    18841 ccccccgtat ctaacagggg ggtacaaaaa cacaaaaaat aagcaagcaa cgataaggaa
    18901 tggctgtcct atcaaggata ataagcaatt tgtgtagatg ttaactgatt tgaaaaagtt
    18961 aacaccttaa atttggaggt gagaacttgg cacaaaggaa tttagattat cggctattaa
    19021 aagaccggcg taatgaatat ggcgtttcac aaaataaatt agctacgact tgtggactta
    19081 gtcgcccgta cttaaatcag atagaaaatg gtggcgtaac cgcatcgaca aaaaccatga
    19141 ggaaaatttt tgaccaactg gaaagtttca atcccgagtt acctttaacg ttactgtttg
    19201 actatgtaag gattcgcttt cccacaacgg atgcgaggaa aatcattcaa gagattctcc
    19261 atttgaaatt tgattatatg ctccacgaag attacgcctt ttactcctat caagaacaat
    19321 atgttatggg agatattgtg gtgatgttgt cacatgaaga agataaaggc gtccttttag
    19381 aattaaaggg tcgtggttgt cggcagtttg aaactttttt actcgcccaa aagcgtagct
    19441 ggtatgactt tttcgaagat tgtctaaaag ccggtggcgt gatgaaacgc ttagacctgg
    19501 caatcaatga tcgagttggc ctgttagata ttcctgattt aacgaaaaaa tgtcagaagg
    19561 aagaatgtat ctccttgttt cgtaccttca aaagctatcg ttccggggaa ctgttgaaac
    19621 ctgatgataa agatggtatg ggcaatactt tgtatattgg cagtcttaag agtgaggtct
    19681 atttctgttt gtacgaaaaa gactatgagc aatatatcaa gttagggatt cctttagacc
    19741 agaccgaaac gaaaaatcgt tttgaaatcc gactgaaaaa tgatcgagct tatcatgcaa
    19801 ttcaagattt gttaaaaggt cgcagtattg aaagtaccac attttccatt attaaccggt
    19861 acttgcgatt tgcggataag gtggaaggca aaaggcgaac taactggccc ttgaatgaac
    19921 aatgggggcg cttcattgga cggaatcaga aggaaattca attaacttct gaaccgaagc
    19981 cttataccat tgaacgaacg ttgaactggc ttggacgaca agtcgcccct acgtggaaga
    20041 tggccaagga gttggatcgg ttgaatcaga caacgtacat tcaggatatg gtaaataata
    20101 ctcgactatc agaccggcac aaaaagattt tggagcaaca aagtatggca attgaaaatt
    20161 tgattatatg aaaagaggaa tcgtgatgga aattgtgaaa gatattttat tggtgtttgg
    20221 tggcagtatg cttggtgtta ctgtgatgtg cttgatgcat gcaagtgcag cggcggaccg
    20281 agcaatggaa aagaaaggaa agtgatgaaa tgaatttcgg tcaaaattt
//
LOCUS       DASJRQ010000052        19746 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJRQ010000052 DASJRQ010000000
VERSION     DASJRQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.052"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            112..1371
                     /locus_tag="U4A80_001687"
     CDS             112..1371
                     /locus_tag="U4A80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1975311.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1460..2407)
                     /locus_tag="U4A80_001688"
     CDS             complement(1460..2407)
                     /locus_tag="U4A80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1975312.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2801..4093
                     /locus_tag="U4A80_001689"
     CDS             2801..4093
                     /locus_tag="U4A80_001689"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1975313.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4108..5877
                     /gene="aspS"
                     /locus_tag="U4A80_001690"
     CDS             4108..5877
                     /gene="aspS"
                     /locus_tag="U4A80_001690"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1975314.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5986..6861
                     /locus_tag="U4A80_001691"
     CDS             5986..6861
                     /locus_tag="U4A80_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1975315.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7127..9010
                     /locus_tag="U4A80_001692"
     CDS             7127..9010
                     /locus_tag="U4A80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1975316.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9085..9369)
                     /locus_tag="U4A80_001693"
     CDS             complement(9085..9369)
                     /locus_tag="U4A80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1975317.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9359..10093)
                     /locus_tag="U4A80_001694"
     CDS             complement(9359..10093)
                     /locus_tag="U4A80_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1975318.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10222..10845
                     /locus_tag="U4A80_001695"
     CDS             10222..10845
                     /locus_tag="U4A80_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1975319.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11037..12158
                     /locus_tag="U4A80_001696"
     CDS             11037..12158
                     /locus_tag="U4A80_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1975320.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12165..15293
                     /locus_tag="U4A80_001697"
     CDS             12165..15293
                     /locus_tag="U4A80_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1975321.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15312..18506
                     /locus_tag="U4A80_001698"
     CDS             15312..18506
                     /locus_tag="U4A80_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1975322.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18652..19359)
                     /locus_tag="U4A80_001699"
     CDS             complement(18652..19359)
                     /locus_tag="U4A80_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1975323.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         6591 a         3480 c         4164 g         5511 t
ORIGIN      
        1 actctaactt aagtggcttt tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa
       61 aaaagttaag cttaaattat acaagttatt ttttatagga ggttgctgaa gatgaaagaa
      121 gtaccagtag tagatatcaa aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac
      181 atgttggagg agttacgcca tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat
      241 aaagaagcta tcacgatcta cggatcatca gcagcaaaag tattttatga tccaaggaat
      301 ttcaaacgaa aaggtgctat gccaaaatta gtattgaaga cactttttgg tcaaggtgga
      361 gtacagactt tggatggagc tgctcatcac catcggaaaa atatttttat ggatctaatg
      421 acaccggaaa gaatggaaga ttatcatcgt atcttagata agaatctgac acaagcactt
      481 gaagcacagc atggtcaatt tgaactattt gatttaagta agatggtttt ctttacatct
      541 atttgcgagt gggcaggtat caatttatca gcgatttcta aagatgaggt cgaaaaatta
      601 gcagaatatc aaatctctat gatcagcgga acgtttactt ctccgattga tcacataaaa
      661 ggtgtcgaaa atcgcaaaaa atcagaaaaa tgggcgcaag gcttgattga agaagctcga
      721 caaaatcctg tggctggaaa agaaaatgtg gcattatatg cttttgccaa tgccacagat
      781 ttagatggtc agcttttacc tctggaagtg gcagctgttg aattattgaa catcatccgt
      841 ccaacggttg ctctaactgt gtgggcggca ttgatgggac atgcactttt cagtagacca
      901 gatttgtatc agcaattaaa aaatgacttt tcgacgttac aagatccgtt tattcaagaa
      961 atgagacgct attatccttt cttcccaatg ctacctgcga tttcgctgaa agaagtagaa
     1021 gtagatggtt atcgtatacc agaaggcagt tgggttattt tagatttata tggaacagac
     1081 catgatgaac gaacagttga agcgccagat tcctttatga tcaaacggta tgtagggaaa
     1141 gcaaaagata tttcctataa agaagaatat gagatgattg cacaaggcgg gggaaatttt
     1201 cgacaaatgc atcgctgtgc aggagaatgg atcactcttc atagtctacg tgtgttttcc
     1261 gatcagctag taaataagtt tgagttctct gtacctgaac aagattggac gattccattc
     1321 aatcagtttc cgacttatcc gaatagccga gcgctacttt acaagaatta gattagcata
     1381 aacttatcga aaaaaaggaa gatcaaacag tgctgatctt ccttttttca tattactata
     1441 aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt tgataatctt
     1501 cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct ggacgcttag
     1561 ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa acaagttgcg
     1621 caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt
     1681 ctctgaaacg ttctccttgg aaatctggat ttctttttcg aggatctcct tcaccaaatt
     1741 gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg
     1801 gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca gcctgccgat
     1861 ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc gcttctttca
     1921 attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct tcttttaatt
     1981 cgtgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag tggatataaa
     2041 aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa
     2101 actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct tttgtagcga
     2161 tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc agttcttcgg
     2221 aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta ttcaaagctg
     2281 tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa
     2341 cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg attcttattt
     2401 ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa aagctgaact
     2461 aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact ggtttttagt
     2521 aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa actttacaag
     2581 agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac actctggaga
     2641 tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa gagaaatctt
     2701 tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat gaacaagggt
     2761 gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc aaagaccaaa
     2821 aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc
     2881 acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt ttgaacacta
     2941 tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag agatgtatga
     3001 tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag caccaatcgt
     3061 tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt
     3121 ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac gtcaattcca
     3181 tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg agagcatggt
     3241 gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag tcatcaattc
     3301 attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact atcttttacc
     3361 ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc cattacgcgt
     3421 attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat ctatcttaga
     3481 ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc tggatgcatt
     3541 ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt acacacatac
     3601 gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga tttgcgcagg
     3661 cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg gcttcggatt
     3721 tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag ttatccctgc
     3781 atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata tcgaagcatt
     3841 gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact tcatgaaccg
     3901 taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag tattggtgat
     3961 cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga
     4021 aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg atgaaatgac
     4081 tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg cggattagtt
     4141 tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa aagacgagac
     4201 cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt
     4261 aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag tgaatacgtg
     4321 atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc
     4381 actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg
     4441 ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat
     4501 ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc gacaacaaaa
     4561 gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc ttacctaggg
     4621 aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca tgctggacat
     4681 ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa tgctggattc
     4741 gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag
     4801 cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga aatccaaact
     4861 tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga agtcacattg
     4921 ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga caaaccggat
     4981 acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt agaattcaaa
     5041 gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct
     5101 gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg acaattcgga
     5161 gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa
     5221 ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc aggtgacttg
     5281 ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc tatccggaca
     5341 cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt atgggtaact
     5401 gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca
     5461 ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc aaaagtctat
     5521 gcagaagctt atgatatcgt cttgaacggt tatgaattag gcggaggttc tatccgtatt
     5581 catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa agaacaaatg
     5641 gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc acatggcggt
     5701 attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa tattcgtgaa
     5761 gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc accaagtatg
     5821 gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga agaatagccg
     5881 ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga tttaattgaa
     5941 aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa aaaaacattc
     6001 gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt ttatgcgttg
     6061 atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc aagtggtatt
     6121 actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt cgaagtaccg
     6181 gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg gttaaaaatc
     6241 gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct ttgtatccat
     6301 attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt tgggggggct
     6361 gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg cggtctagat
     6421 atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat ttcgatctac
     6481 ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata tatgttttac
     6541 agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac taaacaaaag
     6601 aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga aatccaaaac
     6661 tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa gcatgataag
     6721 cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag agaagcaatg
     6781 aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat acttggtcgt
     6841 ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac tgctgtagac
     6901 agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag aaaaactgct
     6961 tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt aaatttatgg
     7021 gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt cacttaatac
     7081 ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg aaaaacaaaa
     7141 aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat tagctgtggt
     7201 gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc
     7261 gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg aatgggaaca
     7321 aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac tctatccggc
     7381 acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc gagggctgtt
     7441 aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact ggtggcaaat
     7501 caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta cgcatatctg
     7561 gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg tattgttgat
     7621 ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat ccttcctctc
     7681 cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg tttattacgg
     7741 taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg tttggccaag
     7801 tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata tcgctggagg
     7861 aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt acaatttcat
     7921 ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg cagtgacagc
     7981 acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt ggattggcaa
     8041 acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg aagcaaaggt
     8101 gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat tgattctaat
     8161 catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg attatggtcg
     8221 tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga gcaaaccatg
     8281 ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa
     8341 aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt
     8401 agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc aagcgatcct
     8461 tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag aactaacggc
     8521 atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt atccaaaagt
     8581 agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt
     8641 aaaggaacaa gatttgttga aagacactgt tctgattgga ctaggaacga atggctcatt
     8701 ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt
     8761 caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc ttgaacagat
     8821 gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta atgaccatag
     8881 tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac attacattca
     8941 cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg agacaaacgt
     9001 gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc tgaagttttt
     9061 ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg ttgagtgtgt
     9121 ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt ctgcttttgt
     9181 tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc ttcttttttg
     9241 aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc tagaaagatc
     9301 ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa aataatgttc
     9361 atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata ttgtaagtga
     9421 tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct tcaactaaca
     9481 aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta ggtgcaattc
     9541 gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg accatagtaa
     9601 agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct aacgtcgtat
     9661 gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt gttggcagcc
     9721 ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga acgacagata
     9781 agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg ttaagcgcga
     9841 tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg atagccgcct
     9901 gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg tcaacaatct
     9961 tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa
    10021 acactttgga actttggata atcttgatat cttctgcgta taattggtcg attcgttcgt
    10081 cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat tatactattt
    10141 ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata ctatgaccat
    10201 aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt tcgagtacta
    10261 ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc gcaagatgag
    10321 aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct ggcggtagct
    10381 gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa tccagttctg
    10441 cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc aggaccaagc
    10501 gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat catgtttccc
    10561 agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc cagaatgtct
    10621 aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga tctgttcaag
    10681 cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat caagaaaacg
    10741 gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga caaattggac
    10801 aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga taagtttatg
    10861 cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa tgaaaacgag
    10921 ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga agacaggtgt
    10981 catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt gattgaatgc
    11041 gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat gacttgattg
    11101 aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa aaagtagagg
    11161 caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat gccgtagaat
    11221 tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt tttgctatat
    11281 caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc agttattcga
    11341 actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt aaaaacgtac
    11401 aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt gaagatgaca
    11461 atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag gagctatttg
    11521 attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt gtaaaatctg
    11581 attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa ctacttacac
    11641 cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga caggataatg
    11701 ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg
    11761 tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc gaacccctgc
    11821 atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc ttttatcagt
    11881 ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc atcccgaata
    11941 tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg gcaaacggtc
    12001 gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct caaaaattag
    12061 cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa gaaaaatggc
    12121 tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat
    12181 tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca cgtttgacag
    12241 aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg atttttgatg
    12301 ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga aaagagatgc
    12361 gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt gaacatcagc
    12421 agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg
    12481 gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa
    12541 taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg atgaatctcg
    12601 atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt aattttttgg
    12661 tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact caatttttcc
    12721 aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt
    12781 tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag
    12841 cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac
    12901 aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag caactagaaa
    12961 attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg atattaataa
    13021 acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa cgatggattg
    13081 ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa gaaataaaag
    13141 ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta gcgatgacta
    13201 cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa
    13261 tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct
    13321 tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa gaagaattga
    13381 atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga attcttcaag
    13441 aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga cagttaaaat
    13501 atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg
    13561 cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat
    13621 tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag cagcaaattg
    13681 ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga tcaacggaac
    13741 accccataca acaattaaaa cacgaacagc caaataagga agatatctta aaagtagaac
    13801 aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt gccaaaagtg
    13861 aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa atcatggcaa
    13921 cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa aaggcatttc
    13981 ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa tctttagata
    14041 gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga caacaagcag
    14101 aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa aataatcagc
    14161 tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat acggatagta
    14221 actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac tcgaatcaac
    14281 tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg cttcggacac
    14341 agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa aaggaacaag
    14401 aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa gaaatctgtg
    14461 catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact tcttatcatg
    14521 aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag ctaactgaga
    14581 cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt atgatcgaaa
    14641 taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg
    14701 atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt acgcaattac
    14761 agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg gaacggtatg
    14821 tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc caacgtttaa
    14881 cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga gggaaaaccg
    14941 gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg cataccctgt
    15001 ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc gatgtgatcc
    15061 agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga tttgggtcat
    15121 tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa agtgaaggaa
    15181 gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta caacaaataa
    15241 gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga taaaaaggga
    15301 gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga cgtcaatact
    15361 tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag
    15421 aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa gagatagatt
    15481 actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt gctgtggaac
    15541 tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca atgctagaac
    15601 cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac
    15661 aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa acgattgaga
    15721 ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc ggttcattgg
    15781 catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa gcttacacag
    15841 aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa cgggcgtttc
    15901 aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg catcaattat
    15961 taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg cttctcccag
    16021 tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg catctgcaga
    16081 taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct ttatcattca
    16141 aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt gattttttct
    16201 ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg aaatatcaac
    16261 aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca gaagtagtgt
    16321 tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg tcagagatcg
    16381 gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc gtcgaattta
    16441 ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg
    16501 aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct tttcaggtgt
    16561 tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat cgctaccaga
    16621 aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat gcctttttcc
    16681 gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga aaattgcgag
    16741 agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag accggttcat
    16801 ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat catgtcctgc
    16861 aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt ctttcttttg
    16921 attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa tcgatacgta
    16981 cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa aatcaattgg
    17041 aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat ctcattcatc
    17101 cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga gattatcaaa
    17161 agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt attttagccg
    17221 atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg gaaaaagaag
    17281 aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac aactggcagg
    17341 cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg aataaaaaag
    17401 aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat gccagcttaa
    17461 gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt gatgaggcac
    17521 aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta gctgtacctc
    17581 agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg tggtcattgt
    17641 ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa cttgccgcag
    17701 aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa actgtgttga
    17761 aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa gaacaaaaaa
    17821 ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag
    17881 cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga caaatttgct
    17941 gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc gaacaagtaa
    18001 aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt tctcaattta
    18061 cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg acattttatt
    18121 taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt aatgctggcg
    18181 aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat ctcacgggag
    18241 cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa caagctgctg
    18301 gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg atctctgaag
    18361 gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg ttccaaaaag
    18421 tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc cgcatgattt
    18481 taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa
    18541 agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag gctgggattc
    18601 cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag gttatacaaa
    18661 gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat cgcaaattgg
    18721 agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt tagcactttt
    18781 tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt ctggaagcat
    18841 atgaaattct ttctcagcca gctgaaaaat ctctttttct ggtttttgga aacctgttgc
    18901 ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt gcttcagttt
    18961 ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat tcttttcctt
    19021 caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa gttcttcctc
    19081 gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt ctaaatcttt
    19141 tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc ctgccattac
    19201 ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat gcatatctgc
    19261 atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct gctgatcata
    19321 catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg cctttcggtt
    19381 ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc taaaaaagct
    19441 gataaataaa ggtttttctt actatatttc tatttccttg acagaataag gtttttatat
    19501 ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa caaattaatc
    19561 attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt tcgaaagatt
    19621 tattttttta atacagtctc aatataacta ttcaaacata aaataacgtt atttgattta
    19681 tttgtttata gtaaatttta tttagtaatc attgctttaa aatactacgc ttatataaca
    19741 gagaaa
//
LOCUS       DASJRQ010000053        19504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJRQ010000053 DASJRQ010000000
VERSION     DASJRQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.053"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="U4A80_001700"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="U4A80_001700"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1975324.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="U4A80_001701"
     CDS             complement(1173..1868)
                     /locus_tag="U4A80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1975325.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="U4A80_001702"
     CDS             complement(1881..2597)
                     /locus_tag="U4A80_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1975326.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="U4A80_001703"
     CDS             2927..3532
                     /locus_tag="U4A80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1975327.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="U4A80_001704"
     CDS             3857..4180
                     /locus_tag="U4A80_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1975328.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="U4A80_001705"
     CDS             4362..5732
                     /locus_tag="U4A80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1975329.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="U4A80_001706"
     CDS             complement(5862..7163)
                     /locus_tag="U4A80_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1975330.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="U4A80_001707"
     CDS             complement(7393..9351)
                     /locus_tag="U4A80_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1975331.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="U4A80_001708"
     CDS             complement(9528..9794)
                     /locus_tag="U4A80_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1975332.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="U4A80_001709"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="U4A80_001709"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1975333.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="U4A80_001710"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="U4A80_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1975334.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="U4A80_001711"
     CDS             13138..13701
                     /locus_tag="U4A80_001711"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1975335.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="U4A80_001712"
     CDS             complement(13954..14475)
                     /locus_tag="U4A80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1975336.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="U4A80_001713"
     CDS             complement(14478..15320)
                     /locus_tag="U4A80_001713"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1975337.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="U4A80_001714"
     CDS             complement(15313..16038)
                     /locus_tag="U4A80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1975338.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="U4A80_001715"
     CDS             16214..16510
                     /locus_tag="U4A80_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975339.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="U4A80_001716"
     CDS             17078..18670
                     /locus_tag="U4A80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1975340.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="U4A80_001717"
     CDS             18683..19423
                     /locus_tag="U4A80_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1975341.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5578 a         4002 c         3701 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttct
//
LOCUS       DASJRQ010000054        18536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJRQ010000054 DASJRQ010000000
VERSION     DASJRQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.054"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..778
                     /locus_tag="U4A80_001718"
     CDS             131..778
                     /locus_tag="U4A80_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1975342.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    907..1123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1171..3816
                     /locus_tag="U4A80_001719"
     CDS             1171..3816
                     /locus_tag="U4A80_001719"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1975343.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4126..5439
                     /locus_tag="U4A80_001720"
     CDS             4126..5439
                     /locus_tag="U4A80_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1975344.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5429..6082
                     /locus_tag="U4A80_001721"
     CDS             5429..6082
                     /locus_tag="U4A80_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1975345.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6137..6829
                     /gene="radC"
                     /locus_tag="U4A80_001722"
     CDS             6137..6829
                     /gene="radC"
                     /locus_tag="U4A80_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN1975346.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6979..7179
                     /locus_tag="U4A80_001723"
     CDS             6979..7179
                     /locus_tag="U4A80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1975347.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7433..9112)
                     /locus_tag="U4A80_001724"
     CDS             complement(7433..9112)
                     /locus_tag="U4A80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1975348.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9114..9326)
                     /locus_tag="U4A80_001725"
     CDS             complement(9114..9326)
                     /locus_tag="U4A80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1975349.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9719..11449
                     /gene="efrA"
                     /locus_tag="U4A80_001726"
     CDS             9719..11449
                     /gene="efrA"
                     /locus_tag="U4A80_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1975350.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11446..13206
                     /gene="efrB"
                     /locus_tag="U4A80_001727"
     CDS             11446..13206
                     /gene="efrB"
                     /locus_tag="U4A80_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1975351.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13301..13498
                     /locus_tag="U4A80_001728"
     CDS             13301..13498
                     /locus_tag="U4A80_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975352.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13651..14136
                     /locus_tag="U4A80_001729"
     CDS             13651..14136
                     /locus_tag="U4A80_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1975353.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14240..14833)
                     /gene="clpP"
                     /locus_tag="U4A80_001730"
     CDS             complement(14240..14833)
                     /gene="clpP"
                     /locus_tag="U4A80_001730"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1975354.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15013..15540
                     /locus_tag="U4A80_001731"
     CDS             15013..15540
                     /locus_tag="U4A80_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975355.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15631..16368
                     /locus_tag="U4A80_001732"
     CDS             15631..16368
                     /locus_tag="U4A80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1975356.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16372..17289
                     /gene="miaA"
                     /locus_tag="U4A80_001733"
     CDS             16372..17289
                     /gene="miaA"
                     /locus_tag="U4A80_001733"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1975357.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17291..18520
                     /gene="hflX"
                     /locus_tag="U4A80_001734"
     CDS             17291..18520
                     /gene="hflX"
                     /locus_tag="U4A80_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1975358.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5992 a         3207 c         4028 g         5309 t
ORIGIN      
        1 taccgcagat gcaagctttt gaacaccgtt ttatttgtct cacacccttt tccttcctac
       61 ttgtaacctt gctcccgtgt tagaaacatg ttacactaaa tatgtgaaat tattaacaag
      121 gagcgtcgtt atgcgagaaa aaacagatac actgccaaaa gcaactgcga aaagattacc
      181 tttatattta cgctacttga agatgctgga tgactcaggt ataagtagaa tcaaatcaaa
      241 tgagttcagc gaaatcaccc aaattccttc tgcaacgatc cgcagagact tctcgcaatt
      301 aggcgagcta ggcagaagcg gatatggcta tgatgtccct tttcttatcg atgtattcaa
      361 taatatcctt aatactaaag aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg
      421 caaagcattg aaacacaaca attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt
      481 tgataatgat ccagcactga tcaatcgagt aattgatgga gaaatgatct atccgattga
      541 tcgtttcgcg gagatagcca aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag
      601 taaatattca caatctgcta ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa
      661 ttttgcgcct gacagagtaa ctgtcccagc ttatgtcaat gttcaataca ttgatttgac
      721 aaccgagttg cagacgctta tttactttga tgagaattac tcagaagttt tttcttagga
      781 aaatttgagt aggcttattg atcttgcgag aactcgtcgt aattctttca aaaatttttt
      841 agtactaaat gaagtggttt tttcacgacc acttgttgac taaaaaaaaa aaccattgta
      901 gaatgaagca taacgctgaa caagaggagt aactggcaca ttcattctta gagagagaac
      961 tatctgctga aaggttctca atgacaacca gtgaaggtag cttgggagtt tctgtcttga
     1021 atggagtaga gatagacgag tcgtgatcgt taactcactt gagaggcaat aatgattgat
     1081 tattgcaatt taggtggtac cgcgtaaatc atacgtccta gaatacgaaa gtattttagg
     1141 acgtttttta gtttaaagga ggaaaacagc atgacggaag aaaataacct atcaacaaaa
     1201 tataatccgc aagaagttga agctggacgt tatcagcatt ggttagaaca agatttgttt
     1261 aaaccgagcg gagataaaaa agcaaaacct tattcaatcg taatcccgcc cccaaatgtt
     1321 actggaaaac ttcatttagg gcatgcttgg gatacgacct tgcaagatat gctgattcgt
     1381 caaaagagaa tgcaaggata tgatacttta tggctgcctg gtatggacca tgcaggaatc
     1441 gcgacacaag caaaagtaga agcgaagctt agagagcaag ggatttctcg ttatgatcta
     1501 ggtcgtgaaa agttcatcga tcaagtgtgg gaatggaaag aagagtatgc gagccacatc
     1561 cgcgaacagt gggctaaaat gggcttatct ttagattaca gtagggaacg ttttacatta
     1621 gatgatggat tatctgaagc agtacgtaaa gtttttgtta ccttatacga aaaaggtttg
     1681 atttatcgtg gtgaatatat catcaactgg gatcctcaag cacgaacagc tctatctgat
     1741 atcgaagtta ttcataaaga tatcgaaggc gccttttatc atatgagcta tgaactagca
     1801 gatggatcag gcgtagtaga aattgcgaca acccgtccag aaacgatgtt gggagatacg
     1861 gcgattgctg ttcatccaga agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt
     1921 ctacctttag tcaataaaca aatcccaatc attgcagatg aatatgtaga tatggaattc
     1981 gggacaggtg tagtaaaaat cactccagca catgatccaa acgactttga ggtaggtaat
     2041 cgtcatgatc ttccacgcgt aaatgttatg gatgatgatg gtacgatgaa tgaactagca
     2101 ggaaaatatg ctggaatgga acgttttgct gctcgtaaag caatcgtgca agatctgaaa
     2161 gaaatcggtc gattgattaa aatcgatacg atgatccaca gtgtgggtca ttccgagcgg
     2221 acaggtgtcg ttgttgaacc tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta
     2281 gcgaaaaaag ccattgataa ccaagaaaca gatcaagcag tggaattctt ccctccaaga
     2341 ttcaatcaaa cgtttttacg ttggatggaa aatgtccatg attgggtaat ctcacgacaa
     2401 ttatggtggg gacatcaaat tcctgcatgg tatcacaaag aaacaggtga aatgtatgta
     2461 gggatggaag caccagcgga tagtgaaaac tgggtacaag atcctgatgt tttagacact
     2521 tggtttagtt cagctttgtg gcctttctca acaatgggct ggccagatac ggaaagcgaa
     2581 gattataagc gatacttccc tactagcact ttagtaaccg gttatgacat cattttcttc
     2641 tgggtaagca gaatgatttt ccaaagcttg gaatttactg gcgaacgtcc atttgagaat
     2701 gtcttgattc atggattgat tcgtgacgaa caagggcgga agatgagtaa atcattaggt
     2761 aatggaatcg atccgatgga agtaatcgaa aagtatggag cagatgctct ccgctggttc
     2821 ttatcgaatg gttctgcacc aggacaagat gtccgcttca gctatgaaaa aatggatgct
     2881 tcttggaact ttatcaacaa aatctggaat gcttcacgtt tcgttatcat gaatatcgaa
     2941 ggaatgaccg tagaagagat cgatttatct ggagaaaaat cagtggctga ccggtggatc
     3001 cttactcgtt tgaatgaaac gatcgaacga gtaactgagc tatttgatcg ttttgaattt
     3061 ggtgaagcag gtcgacaact gtataacttt atctgggatg atttttgtga ctggtacatc
     3121 gaaatgagta aagaagtact ttatggagat gatgctgttt caaaacaaac cacccgtagc
     3181 atcttagtcc atacacttga tcagatccta cgtttattgc atccaatcat gccattcgtt
     3241 acagaagaaa tatgggaaca tattcctcat cagggaaatt ctctagtagt ggcagaatat
     3301 cctgttgtac gtccagagtt tgatgatgaa acagcttcta aagggatgga agtattgaaa
     3361 gaattgattc gagccgttcg aaatattcgt tcagaagtga atacaccatt gtctaaacca
     3421 attacgttgc taattaaaac aaacgatgct aaaatcgatc aatttttagt tgaaaataca
     3481 agctatatcg aacgcttctg taatcccgaa gagttgacga tatcaagtga gatcactgcg
     3541 ccagatctag cgatgtcagc tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga
     3601 ttgatcaata ttgaagaaga aatcaaacga ttagaaaaag aactggaaaa gtggacaagc
     3661 gaagtaaaac gtgtccaagg aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa
     3721 gaagtagtag aggcagagcg tgcaaaagaa aaagattatt tagagaagca aacggctgtc
     3781 aaagaacgaa tcgaaagttt gcgtactatt caataagaat agaaaaagag agtgtgaaat
     3841 cagctagcta ttactaaaag ctagctgatt gaacatcgtt acaggaaaga gtttgagcct
     3901 cactcttagc ttcgattcat aaggagatgt gacataaatt ttgtcgcatc tcttttttag
     3961 tctactctta ttatgtagga gaaggaggga ggtggatcat gtatcaagag tcaataaagt
     4021 agttgataaa atcgttcctc tctctgtttc ttttctcttt gtcatacttt tatttttctt
     4081 atgtgttcta tttgttataa taaaagagaa atgggggaat tgttgatgac gattgaagaa
     4141 gcaatagaat ggatccatag tcgattacca tttggttcac gtcctggcct tgaccgagtc
     4201 gaagctttat tagaagtggt agggcatcca gagagaaaag taaaaacgat ccatatcgct
     4261 ggaacaaacg gaaaaggttc tactgtagct tatttgagaa gcctattaga agaggctggg
     4321 ctgacagtag gtacatttac ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc
     4381 aatggcaaaa gcattccaga caatgatttg atccagcttg taaaaaaata tcagccactg
     4441 atcaaacaac ttgatcaatt tgaagcagta gctggtatta ctgaattcga gacattaaca
     4501 gcaatggcat ttgattattt tttgatgaag aaagtggacg tagcgattat cgaagttggc
     4561 ttaggcggct tattggattc taccaatgta gctgtaccaa tgctgacggg aataacaacg
     4621 attggacttg atcataccga aatacttgga gatacaattg aagaaatcgc tgcacaaaaa
     4681 gctggaatca tcaaacaagg cgttccagta gtaacaggaa atattgcccc agaagctttt
     4741 actgtcatcc aacaaacagc taaagcaaaa aatgcaccgc tttatgtata cggggaaaat
     4801 tataaaataa actatctgca tcctgatgag atgtggggag aagtatttga attttttaat
     4861 gaaataggaa agatccctca attaaccact ccgcttttag ggcaacatca agctgaaaat
     4921 gcaggagtag ccatcgaatt gtattttgtt tactgtcaat tggaaaagct gcctttccga
     4981 gaaaaagatg ttttgaaggg attgaagaaa tctcgatggc caggaagaat ggagcgtttg
     5041 agtaacgagc cattagttgt tttagatggt gcccataata cgcatgcaat gaaccggctt
     5101 gttgagaatt tgaaaaaaga attcaaagga tatcaaatca acattttgtt ttccgcctta
     5161 cgaactaaaa atgttagaga aatgctagaa cgattgcagc aagtaccaaa tgtccatatt
     5221 tatttgacga cctttgaaca tccaaaagcc atcgaattaa gtgaagtatc agatttagca
     5281 agtgagagag tatcaatcgt ttcattgtgg caattcggat tagcagatat attggaaaag
     5341 atgacaaatg aagacgtatt agttgtgacc ggttcacttt atttcatttc agaagttcgc
     5401 catttattaa tacaattagg aggagatcat gaagtataat gcagtgattt ttgacatgga
     5461 tggtttgctg ttcgacacag agttagtgta ttacgaagcc acacaagtag tagcagacaa
     5521 gatgggtttt ccttatgata aagacctgta tttaaaattc ttaggtgttt cagatgaaga
     5581 agtatgggcg aattatcata tgattttctc ggaatttggc aaaacaagtg tacaaagatt
     5641 catagatgat tcataccagg aaacgcttga acgtttttcg caaggagcgg tacaaatgaa
     5701 gcccggtgtg ttggaactcc tctctttttt agaagaacat cagattccca aagttgtcgc
     5761 ttccagtaac caacgagcag tcattgaact tttattgaag aagaacaatc tgtttgaacg
     5821 atttgagacg attgtttctg cagaaaatgt tcaacgagca aaaccagacc cagaaatttt
     5881 cttgttagct aatcagaagc taggtacagc gaagaaagaa acattgattt tagaagattc
     5941 acaaaatggt attttagcag cttctgcggc tgagatcccg gtagtcatgg taccagatct
     6001 gcttccacca tcccaagaat tgatcaaaaa aacagctggg gttgtttctt ctttacatga
     6061 aatccctgca ttgttaaaat aaaaatccat ctcttttcgt atcttatttt agataagata
     6121 gaaaggagat gtttttttgg gaaaacgatt gatcaaagaa gtacctacta gctctttacc
     6181 aagggaacgt atggaaattt atggcgcgga agctttatcg gatcaggagc ttttggcaat
     6241 cttactgcgt accggtcaac acccttatag tgtgatgtcg attgctggaa atctgttaaa
     6301 aacgtttgga ggactggcct ctttacgaca ggctactttg catgagttag aagagattcg
     6361 agggatagga cgcgtgaaag caatcgaaat caaagccttg atcgaattag gtcgacgaat
     6421 tcagacggat catgaaagag cggctccacc ggttcgtacc agctacgaat tggcccagca
     6481 attgattctg gaaatgaaag atcacaaaca agaacatttg gtttgtattt atttggacac
     6541 gaagaatcaa gtgatcttaa aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc
     6601 tcatcctaga gaaattttcc attatgctgt acgttattgt gcagcaagga tcgttttagc
     6661 gcataatcat ccttctggta atgttattcc atcgcagcag gatatgaatt ttaccaaaag
     6721 gatacaaaag tgtggagaaa tgatggggat cactgtactg gaccacttga tcatcggacg
     6781 caaacgatat tttagtttga gagaagaagg aatgatggaa gagaagtaag ttgctttctt
     6841 gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt tgtttgggaa tagaagttga
     6901 aacatctgtt gcacctctat cgtacacgag caatgttaca atttaaatta gtgcttttgg
     6961 cacgaggagg atattattat ggaaaacgga acagtaaaat ggttcaacgc tgaaaaagga
     7021 tttggattta tctcacgtga agatggaagc gacgtattcg ttcacttctc agctatccaa
     7081 ggtgacggat tcaaaacatt agaagaaggt caagcagtga ctttcgacgt tgaagaatca
     7141 gaccgcggcc cacaagctgc aaacgttgta aaagcttaat tttatctgta aatcaaattg
     7201 atggtagaca tcaattttta cgagagaaca aagcgagtaa caaaaatacc tgccacaaga
     7261 gacaatttct cttgtggcag gtattttttt gtatgaaaaa gtaaataaat ttaagaggct
     7321 atgaaagagc caccttgtat caaggaataa gaacaaacaa aaatggcttc tcatgttttt
     7381 catttgtttt ctcttatatt caggacaagg tgctgatttc ataacctctt atttattctt
     7441 catctggtgt cattaccatt ggcaaaatca ttggatgtct ttctgtacgt tcgaataaga
     7501 aaggctgaag cgcgccagtc atcgcttctt tcaatttata ttctgaacag tcaggtccat
     7561 tcaaacattc tcttaaagcg tggaataaga ttcgctgagc ttcattgatc atttctcctg
     7621 attctcgcat gtagacaaat ccacgagaga gaatatctgg accagccaag atttcttttt
     7681 tcttgatatc cactgtcgcg acagctaata caagaccttc ttctgaaagc aggtgacggt
     7741 cacgtaaaac gacattacca atatcaccga tcccgctacc atcgatatac acatctccag
     7801 ccacaaaatg cccagcagga cgcgcagaat cgctagttaa cgctaaaaca tcgccatttt
     7861 ccatgatgaa gcagttttct gcaggaacac ctacatcttg tgctagagaa gcatgtactt
     7921 tcaacatgcg gaattctccg tggacaggaa taaaatattt tggcttcatc aaacgaagca
     7981 ttaatttttg ttcttcttgt ccaccgtgac cggaagtatg gatgttattg atttttccgt
     8041 gaatcacttc tgcaccagct tcagacagca aattgatcaa atgattgaca cttgttgtat
     8101 taccaggaat aggtgaactt gaaaagatca ctgtatcgtc tggctggatt tggatttgac
     8161 ggtgagtacc attcgcgatg cggctcaacg cggccatcgc ttctccttga gaacctgtac
     8221 ataggatcat cgtttcattg gcaggcaagc gattgatttc gtgtgcatct acaaatgtgc
     8281 cgtctggaac tttgatatat cctaaacgtt gaccgttgac gatggcattt tccatgcttc
     8341 gtccgaaaac agcgatttta cgaccagttg ccacagcggc atctgctgct tgctgcaaac
     8401 ggaagatgtt agaagcaaag ctggcaaaaa tgatacgtcc ttcgattttt gaaaagatct
     8461 tcaaaataga atgcccaatc gttttttctg atttagtaaa tgttggaatc tcagcatttg
     8521 tactatcaga taaaaggcac aagactcctt cttccccgat acgagccatt ttatgcaagt
     8581 ttgctggttc gccaacagga gtgaaatcga atttgaagtc tccagtcgct acgatatttc
     8641 cagaaggtgt tttcaccacg atccctaatg catctgggat actatgggtc gttctgaaaa
     8701 agctgacaga agtctttcta aaacggatga tcgtatcttc attgatctca tggagctctg
     8761 catctcgaag caagccatgc tcatctaatt tgtttgtgat caacgctaat gcgagaggac
     8821 cagcatagat aggaatattt gcttgtttca gcaagtaggg tattccgccg atgtggtctt
     8881 cgtgcccgtg agtaatcacc aaggctttta ctttgtgtaa gttttgtatg atgtagctgt
     8941 agtcgggaat gacatagtca attccaagta aatcgtcttc cgggaatttg atcccggcat
     9001 caatcaaaat gatttcatct tggaattgta ccccatacgt atttttcccg atttcaccta
     9061 atccaccaat agcaaagacg cctgtctcgt tatttttgat ggaaagtttc atattaaaac
     9121 tccactaatg agaaatcagc attttctttt tcgtactcta gatgacttcc ttctagaagt
     9181 tgaacgaatt caatattgta tggggtattt tcttccacac gttggcgagc gtctacatcg
     9241 ctttttgcct caatgtaaag agattttgtt tcttcccgtt tagggttgcg tacttttgtt
     9301 tcttgataat aaactttata gatcattgtt ttgtctcctt tatctgcgta aagcagtgat
     9361 ttagctagtt gattttttta gacaacacaa aacgagggga caagccacct ggtttttcat
     9421 atgctttgac tagaatactg gattcgagtc aaagagacaa gcctcgtttt tgcttatctg
     9481 ttcggatatt ttttcaaccg taccttctgt ataaaaaata tacaacaatt aattgttatt
     9541 ctagcataaa acagcaaata agtatagaaa tcaactagat tgaaagaaaa aatcaatgtt
     9601 tatcatcccg ctataatcat aatgactttt ttatgagaga agtgttctat taataggctc
     9661 gttgtgctat catttttagt aatgaaaatg taacaaaaga ttaaaaggag atgagaaagt
     9721 gaaactaatg tggcattata ccatgcgtta taagaaattc ttgtttttca atttcatatg
     9781 cgtatttggt tttattttga ttgaattagg acttcctacg attttagcac gtatgatcga
     9841 tgtaggaatc cgcaatgatg attttgatta tgtgaagcaa caagggatat ttatgatcgt
     9901 tattactgta atcggtatta ttttgaatat cttgttaggt tattttggtt cgaggattac
     9961 gactaatatc gtagcagata ttcgagatga tttgttcgaa aagatccaaa gttattcgca
    10021 tcaggaatac gaaaatctag gcgtatcttc attgatcact cgtacaacaa acgatgctta
    10081 tcagatcatg ttgttcctgc aaaatatttt acgtatcggt tttatggcac caatgatgtt
    10141 cattgtcagt ttgtacatgg tcatgcgtac aagtgtgaca ctcagtttgt atgtagtagg
    10201 tgcattacct ttactattat tggcagtcgt agcgattgct aaggtgtctg aaccgttatc
    10261 taaaaagcaa caaaaaaact tagataaaat caacagtatc ttgcgggaaa atctttcagg
    10321 attgcgtgtg attcgtgcat tcgtcaatga aaagtttgaa gaaaaacgtt ttgctgtagt
    10381 caatgagaat tatgcttcta gttcaaaaag cttattccgt ttgatggctg ttgcacaacc
    10441 gggtttttat ttcttattta atatcgtaat ggtattgatc atctggagcg gggcagtgca
    10501 aatcgatgca ggcgagcttc gtgtaggaga tttgattgcg tttatcgagt atatattcca
    10561 tgccttgttc tcattcatgt tatttgctag tgtgttcatg atgtatccaa gagcagctgt
    10621 ttcagctgga cgtatccaag aagccctaga tgccaaacct tcgattgtag aagatccaaa
    10681 tggtatatct gatacaaaaa ctaaagggta tatcgaattt caaaatgtaa catttgctta
    10741 tccaggccat tcgcaagagc cagtgatacg aaatgtcagt tttacagcct ctccaggaga
    10801 gaccgttgct tttattggaa gtaccggtag cggaaaatct acgttgatcc aattgatccc
    10861 tcgcttttat gatgtgagtg aaggaaagat cttgatcgat ggagtagacg tgagagatta
    10921 tcggctaagc aagttgcgag ataagattgg gtttattccg caaaaagcgc tgctatttac
    10981 tggaacgatt gcagataatt tgcgatacgg gaaagaagat gctacgcaag aagaaatgga
    11041 acgtgcagct gagattgctc aagcttcaga attcatttct cgaaaaccag atggatttaa
    11101 tgaacattta tcagaaggtg gttccaattt ttcaggtgga caaaaacaac gattggcaat
    11161 agccagagcc atcatacgcc gtccggaaat ctatattttt gatgacagct tttctgcatt
    11221 agattatcaa acggatgcga agttacgtgc tcgtctgaaa aaagaaacga cagaatctac
    11281 tgtattgatc gtagcacaac gtgtgggaac gatcatgcat gcagataaga tcatcgtatt
    11341 aaatgaagga gaagtcgtcg gtatggggac gcatcgggag ttgttggaaa aatgcccgat
    11401 ttattatgat attgccgctt ctcaattatc aaaggaggaa ttagcatgaa aaaaaccttt
    11461 tcttccttaa aacgtttagc tcgatatatc agaccgtatc gtgagacttt tcttttagtg
    11521 atcctattta cagttctgac agtggctttc aacgtggctt tgccatatgt tacaggactt
    11581 cctactacag aaatcagccg taatattgcc aatggcgaat caatcaattt ttcttatata
    11641 ttcaaatgtc tcatttggat cacagttgtt ggaatagggt attgtatttc gcaattactt
    11701 tctggtgttt tgatgacaaa tgtcgttcaa agtgccatgc atgatttgag aagagacata
    11761 gaagaaaaaa tcaatcgtct tccggtttca tatttcgata aaaaccagca aggaaatatt
    11821 ttgtctcgtg tgacgaatga tgtagatgca gtcagcggtg ctttacaaca agcttttatt
    11881 ggtatcgtaa atgcgatatt gggaattacg ttggctgcag caatgatgtt ttatatccag
    11941 ccgatgatgg cgctgatctc tatgatcatg attccaacat cgatctggat ctcaaaacga
    12001 gtgatcaatg cttcgcagaa atattttcaa agcatgcaga attctcttgg tgagctaaac
    12061 ggctacgtcc aagaaaatat gacaggtttt agtgtattga aagtatatgg acgcgaaaaa
    12121 gaaacattgg aaggattcaa aaaagttaat cacagcttga aatactttgg gtttcgtgca
    12181 gcattcattt ctggcttgat gatgcctcta gttcaattaa ctgcctatgc tacttatatt
    12241 ggtatggctg ttctaggaag cttttatgtt attactgggg cgatcgttgt cggtcaacta
    12301 caagcattca tccaatatat ttggcaaatc agtcagccaa tggggaacgt gacacaattg
    12361 tcttcagttt tgcaaagtgc atcagcggca acgaaacgtg tattcgagat tttagatgag
    12421 ccagaagaaa aagaaaacaa agtagatgct tcactgccag aaacgatttt aggagatgtg
    12481 acattcgatc atgtcgattt tgcatatgat ccgaagaaac cattaatcca agatttgaat
    12541 tttgaagtga aagctggtca aaccgtagct gtggtcggtc cgaccggtgc cggcaagacg
    12601 acattgatca atctattgat gcgtttttat gacgtagaca aaggagcaat aaggattgat
    12661 ggaatcgaca ccaaatcaat gagtcgcagt gatgtgcgtt cgctatttgg tatggtcttg
    12721 caagatgcat ggctttatca aggaacgatt gcagataata tccgcttcgg aaagcttgat
    12781 gcgacagatt atgaagtagt tgatgctgcg aaaacagcaa acgtagatca ttttatccga
    12841 acgatgccag atggatatga tatggccatc aattctgagg gagacaatgt gtctttagga
    12901 caaaaacagt tgctgacgat tgcccgagca gtggtctccg atcctaaaat cttgatttta
    12961 gatgaagcaa caagttcagt agacacccgt ttagaagcgt tgattcaaaa agcaatggat
    13021 cgcgtgatgg agggacggac aagtttcgtc atcgctcacc gtttatcgac aatccgtgat
    13081 gcagatctga ttctagtgat ggatcaaggt cagatcattg aaaaaggaac gcatgagagt
    13141 ttattggcac aaggtggatt ttatgaaaaa ctgtataaca gccaatttgc tgaggaagca
    13201 gaataagtca tataagaata aaacagttta aaacttattt tttgttctct attcttgata
    13261 atggtaaagt aaagttatca agaaaagtga ggacacggag atggaacagt ttttaaatga
    13321 aaaaattact gaagccaaaa gagaagttga tcgtttaacg cacgtctgtc agacgaagcc
    13381 ttttgattca gctgattttt ttgaaaatga gtttcaaatc gaacgcttgg cagcacaaat
    13441 agaagtgtac gaattaattt taaaaaaagt aatagaagaa aatatagatg tacaataaaa
    13501 acaggtaaaa cgtcagttac cagatactgt aaatagacat ctggcctgag caggttttac
    13561 ctgttttttt tcttatgaaa taaatatatg ctatgatacc tttattcaat aggattagtt
    13621 tctacaaaaa atgaaaggag aaaaataaat atggaacggt ttatagcatc aaatgccaca
    13681 gtgattggtg atgtgacatt gtcagaagat gtgacgatct ggtatcaagc tgttttacgc
    13741 ggggacagta attggataaa aataggacaa agaacgaata tccaagatgg tacaatcatc
    13801 catgtcgatc atgatgcacc tgtagatatt gcggagaatg tgacggtagg acatcagtgc
    13861 atgctacatg gctgtacaat cgaaaaaggg gcgctgatcg ggatggggac cacgatattg
    13921 aatcatgcgg ttattggtga gaatagtttg attggtgctg gatcattagt aacagaagga
    13981 aaagttattc ctcctaatgt cttggctttt ggtcgcccag ctaaagtgat ccgtcctttg
    14041 acaaaagaag aaatacaaaa aaacaaagaa aacatccagc attatgtcga gataggtcgg
    14101 atgcatgcgc aaggaaaatt caaggagcaa agataggcag tagcatccat aaaataaaaa
    14161 aggatggtca gtttgagttg accatccttt tttatttggt ttagataaga gagcttaaaa
    14221 aagcttcctt tgaaacagat taatttaatg aagaactgtt ttccatgact tcatcgatca
    14281 atccatatgc ttttgcttgt tcagctgtca tatagttgtc acgatctgta tctttttcaa
    14341 tcacttcaag cggttgtcca gtgcgttcag ctaaaatttt gtttaagcgt tcgcgtgttt
    14401 gcaaaatatg gcgtgccgca atttcgattt cagttgcttg cccttgagcg ccgccaagag
    14461 gttgatggat catgatctca gcatttggta aagcaaatcg tttacctttt gtaccagctg
    14521 tcagcaagaa gcttcccatt gaagcagcca tacccataac aattgtttgg acatctgctt
    14581 tgacaaagtt cattgtgtcg taaatagcca tacctgctgt aactgaacca ccaggagaat
    14641 tgatataaag atagatatct ttttcagaat cttgagcgtc taagaaaagc aattgggcga
    14701 tgatcgaatt agcaagatca tctgttactt ggccgcttaa catgatgata cggtctttca
    14761 ataaacgtga gtagatatcg taagctcttt cacctcgtga agattgttca atgactgtag
    14821 gaattaaatt cataatagag ttcctccttg atttcgttta gaaatagtgt accataattt
    14881 agtttctaat acctaaaatg tagtatacat ttttggtcaa aaaaggtcaa ataaaaaact
    14941 taggcaaatt agtcgcataa acccgcttta tggtatgata agcagagaaa caaaacaagg
    15001 aggaacaaca gattgatagt tggtattact ggccttatcg gagtgctgac aggtgctgta
    15061 gttgtctata tcggcatgat actgcaaatg cgtcacttgg aaaataaaga acagcgtaga
    15121 cgtgaattgg aattgaaagt caaagaaatc gagacgctga atcaattaaa taaaaagatc
    15181 aacgagatct tgcaaaaacg gacaacgctg atggaagaat atgtcagctt tgatgcattt
    15241 gatgattgtt atatcacgat tgacgatttt gcgtatctcc aatcattttc agcgcaaaat
    15301 agtttttatc tgccaaatta tattttagac gagctattta agaatatctc ccatcgaaaa
    15361 gtgattttat ctccagatga aacaatgaag attggtggat atgcttataa aggcggacgc
    15421 gtgattttag agaattttac ggatagtctg actgaaatga tcaatgaaaa aaaagctcag
    15481 ttgaagacta tgacgcgcca gccgattggt ttcttctcga aagatgatgt ttggagctga
    15541 tattttgaag cattgccgtt gcacatgaga gcgtggtaat gctttctttt tttcttaatg
    15601 atacaataga actgaacgga gtgaagaaag atgttacaaa tctatgccca tcgcggaagc
    15661 agcggcaccc acccagaaaa tacgcttcct gcatttgcag aagctgtacg agtaggtgca
    15721 gatggtatcg aactagatgt ccatctaagc aaagatggtt atttgatcgt tatgcacgac
    15781 gaagaagtgg accggacgac taacggtaaa gggctgattc gagaaaaaac attggaagaa
    15841 ctcaaaaaat tgaatgcagg gggcaaattc agcaaagaat ttccagcagc taaagttcct
    15901 gaattgagag aagtgttagg actgctcctc caaaaaaatt accgagggat gctaatgatt
    15961 gaaattaaaa ctgatcagta tgaatatccc ggtatcgaag aaaaattagc cgaaacatta
    16021 aaaagtagag attggccttt tcaacatgca tattgcagct ttcatttgga ttctttggaa
    16081 aaaatcgcat tactggaacc ggagatacag ctggacttat tgatgagtac ttctgataaa
    16141 aagatccaac tagcagcaga aaccccttat atagaaggag tacatccaaa aaatacttgg
    16201 ttgttcgaac atccagtcca ggcagctgat tttccaaaag caattcgtgc atggacgatt
    16261 aacgatgaag aagaaatggc caaatgtcaa gaactcaaaa tagatggaat aataactgat
    16321 tttccggaga aagctcttcg gtggaaacaa tcaacgaaaa gtaggtaaaa tatggaaaaa
    16381 gtattagtga tcgtcggtcc gacagcagtc ggtaaaacag cgctaagtgt ggaactcgca
    16441 caactatttg gaggagagat catcagtggt gattcattgc aaatctataa aaatctggat
    16501 atcggtacag caaaaatcaa agaagaagag atgaataatg ttccgcatca tttgattgat
    16561 gtgatcgaac ctactcaacg ttactcagcc gttgattttc agcattcagg tcgacaattg
    16621 attaccgaga ttgcgaaccg agggcaccta cctatcatag ctggtggtac gggtctctat
    16681 atccaatccc ttctatatga ttatcagtta ggagcgacag aagaagcgaa tcctgagatt
    16741 agaaaaaagt atgaaacgtt agctgaaaca attggttccc aagcattatg gaatcagttg
    16801 aaagaaaaag acccggcagc tgctgagaaa atccactgga acaatcaacg caaagtgata
    16861 agagctctgg aagttttaga gacgacagga catagtatca cggcacctaa agaagagcca
    16921 aagcaattgt atgattacct catgattgga ttagatacga agcgtagtat tctttatgaa
    16981 cgaatcaatc aaagagtcga tttgatgcta gcagaaggtt tagtagcaga agcgcgtaag
    17041 gtgtatgagc ttggagaagt ccaggccagt caaggtattg gatataaaga atttttccct
    17101 tattttgaag ggaaagaaac attggaagaa gcaacagaac aagtcaaact acattcgaga
    17161 agatacgcga aacgtcagtt gacgtggttt cgtaaccgtt tggatgcaca gtggtttgat
    17221 cttataagtg agccaaatca aaaaagtcag attgagcaaa caatcaaaaa atggctggag
    17281 gcggaataaa atggaagaaa aagtgatcat cgtaggggta gaaaccgaag caaaccagcg
    17341 ttacttttca gagtcaatgg aagagctagt tcagctgacg aataccgcat caggagaagt
    17401 cgtattcaca attacacaaa agagaccaca agtggatcgg caaacgatta tcggtaaagg
    17461 gaaattacaa gaattagttc aacaagcgga tgcgcatgaa gcggatttga tcatctttaa
    17521 tcatgagcta actccgagac agagccaact gattaccgat gctgttggtc ttccggtaat
    17581 tgatcgcgtg cagcttatct tagatatttt tgcgatgcgt gctcgttcga aagaagggaa
    17641 gctgcaggtt gaacttgctc agttagaata cctgctgcct cgtttagtag ggcaagggaa
    17701 aacattatct cgtcttggcg gtgggatcgg aacaagaggg ccaggtgaaa caaagttaga
    17761 aacagaccgc cgacatatac ggaataaaat ttttgctgtt aagaaagaat tgaaagaagt
    17821 agaagctcat cgagagcgca atcgccaaaa aagaaaaaat agcgagattt ttcagattgg
    17881 acttattggc tacacgaatg ccgggaagtc aactatcttg aatctattga cacaagcgga
    17941 tacttattcg aaagatcaat tgtttgcaac gttagatcca ttgactaaga gatggcgttt
    18001 tgctgaagga tttgagatta ctgtaacgga taccgtcggg ttcatccaag acctgcctac
    18061 gcaactgatc gatgctttcc attctacatt agaagaaagt caaaatatgg atcttttgct
    18121 acatgtagtc gatgctagtt ctccagatcg tattttacag gagcagactg tcttaaaatt
    18181 gatggatgaa ctgaatatga aagaaatgcc gatactgact gtttacaata aggcagacca
    18241 gatcgaccca gcgatgttca cgcctacgct attcccaaat gtattgattt ctactcagac
    18301 gaaagaaggt aagatggcgt tgatcgaagc aatcaaacgt cagctgatgg aactaatgac
    18361 cccttataca ctttctgtgc caagcaacga aggacagaaa ttaagcgagt tgagaagaca
    18421 aacaatggtt ttggacgaag cgtacatgga agaaacaaac gaatatgaag ttaaagggtt
    18481 tgcaaaaacc aattcaaaat ggttaagaaa aaaccaataa aattttattt ttttaa
//
LOCUS       DASJRQ010000055        18476 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJRQ010000055 DASJRQ010000000
VERSION     DASJRQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.055"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..424
                     /locus_tag="U4A80_001735"
     CDS             191..424
                     /locus_tag="U4A80_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975359.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            844..1515
                     /locus_tag="U4A80_001736"
     CDS             844..1515
                     /locus_tag="U4A80_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1975360.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1568..3544
                     /locus_tag="U4A80_001737"
     CDS             1568..3544
                     /locus_tag="U4A80_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1975361.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3559..4311
                     /locus_tag="U4A80_001738"
     CDS             3559..4311
                     /locus_tag="U4A80_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1975362.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4327..5088
                     /locus_tag="U4A80_001739"
     CDS             4327..5088
                     /locus_tag="U4A80_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975363.1"
                     /translation="MRNRKWLFVCAAALFTFTAYTQLTVADSQESTDSTEQTIQTSDS
                     SAIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5101..5361
                     /locus_tag="U4A80_001740"
     CDS             5101..5361
                     /locus_tag="U4A80_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975364.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5358..7448
                     /locus_tag="U4A80_001741"
     CDS             5358..7448
                     /locus_tag="U4A80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1975365.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEVSAQVIDKSKGKDITSEGTLTYDSNLRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7475..7939
                     /locus_tag="U4A80_001742"
     CDS             7475..7939
                     /locus_tag="U4A80_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975366.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7936..8178
                     /locus_tag="U4A80_001743"
     CDS             7936..8178
                     /locus_tag="U4A80_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEN1975367.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8196..8498
                     /locus_tag="U4A80_001744"
     CDS             8196..8498
                     /locus_tag="U4A80_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975368.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8568..10628
                     /locus_tag="U4A80_001745"
     CDS             8568..10628
                     /locus_tag="U4A80_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975369.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMN
                     NQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQFSQADSVATTASIKEEA
                     QEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSES
                     NEVK"
     gene            10628..13237
                     /locus_tag="U4A80_001746"
     CDS             10628..13237
                     /locus_tag="U4A80_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN1975370.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVKIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKREETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNLLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLH
                     DQLEEKNEENMFYKIVQSLENETAQKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIG
                     KRLLLEAEKAVKE"
     gene            13234..15765
                     /locus_tag="U4A80_001747"
     CDS             13234..15765
                     /locus_tag="U4A80_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975371.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15815..16147
                     /locus_tag="U4A80_001748"
     CDS             15815..16147
                     /locus_tag="U4A80_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975372.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16148..16741
                     /locus_tag="U4A80_001749"
     CDS             16148..16741
                     /locus_tag="U4A80_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975373.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16904..>18476
                     /locus_tag="U4A80_001750"
     CDS             16904..>18476
                     /locus_tag="U4A80_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN1975374.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6850 a         2894 c         3774 g         4958 t
ORIGIN      
        1 agatccaaga ttctataatt tttttcaaag taaaaagaaa ttaggtaatg attctcagac
       61 caattactta ggaaaataac catacaaatt agattttaca gataaaggat tagaaggaat
      121 cacggcgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat ttatttagga
      181 ggaaaattag atggcgcgac cagttttaga tgcgatggat atggatactt tgacaaaagg
      241 aaggtatgta cagctgttag ctcgtggtta ttcacctaaa gatgtattca aagccctatc
      301 taaaggaaca gatatggaaa aagaaagatt acgaaaagaa ttcgactatt ggagagagca
      361 taacggaata aaagatttaa aaccagcacg tccagttatt agacgaaaaa aattgaagaa
      421 gtaagaccaa agcgtatggc gtacataggc ttttttattt tgataaaaat aatgaaagga
      481 aggaaaactc atgaattgta aagaaatcga aaatagaaaa aaagtgtcga aagaaatgga
      541 agaaaagtta ctaaaaacaa tgaaacaaaa acaatgaaac aaaaacaatg aaacaaaaac
      601 accttaaaag attatctgtc gcgcaatata tcaatgatat gcagttaaca ggaaaagaga
      661 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gagacgaact tatgtgaaaa
      721 aaagttctaa ttgtcaacta ttgttagaag tctcatgaca ttcattaata gaattcattt
      781 ttaaaacttt ttctatggaa atgagaaaaa gtttttttgt tttgtctgaa aggaggcaga
      841 tggatgaaaa aatggttatt tggtttttta ggagtcgctt taatcgtctt ttgcagtgtg
      901 tttggctatg ttagctacca aaaacatgaa ggagatgtgt ttaagcaaaa tatagaaaaa
      961 aagatgcccg tagatcaaat caatgcacac gcaaagagtt ataaggagga tgcaacgaac
     1021 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aaaaagaagc aatcgaaatg
     1081 ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt agcattgccg
     1141 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgag
     1201 gatgccgaaa tgggcaaagg aaactatgtt ctagcaggac acaacatgga aatgccaggt
     1261 gttttatttt cagatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
     1321 gatggcgtct atcgttacaa ggtaacgtgt aaatttatcg tatctgagta ctttaaatta
     1381 attgatggcg ttcctgaaga gaactctttc ttgtctttac caaagaaagg agagaaaccc
     1441 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     1501 ggagagctac aataacaagg ttgtaatcaa cgcgagtcgt tgaaatatga aatgaggaga
     1561 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttgttg gaatgttagg
     1621 aatcattgca ggctgctttc agggatatcg agcatatgca gaagatgtca cgcaaaaaac
     1681 gccaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
     1741 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
     1801 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     1861 ggatcaagta ttaccgactg aagattctga tctaaccaac gcgaaagtgg acgaaatcgt
     1921 taaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaataaaat
     1981 tacttcagct gtagatgaaa atggtgagat cacttttgct gatcaaccag cctatgtcaa
     2041 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     2101 aaaagcaaaa ccaatggttg tgattgcgcc aatgactgat aatacaggtt caggtttttt
     2161 aaaagacatt cacttatatc ctaaaaatat tgtttctaag ttgagttttg aattaacgaa
     2221 atttggtgac gatggcacag ctcaaagcaa acaaacacct ttaaaagggg caaaatttga
     2281 gttatacaag ggcgaacctg gaaaaggaac aaaattagga gatcttgtat cggatgatca
     2341 aggaaagctg actgctactg atttgacgtt aggaaaatac tattttgtgg aagttccttc
     2401 agaagttgta gtgggatcag ataaagaacc aacagcagat caatatttat taggcgcaga
     2461 cgctcgcaac gatgcgcaca ataaattaac ctttgaaatt acgaatgatg gcgtgacaag
     2521 tgacttaaaa gcttcatacg taaactataa agcaccagtg atcgataaaa cagtaacaaa
     2581 tggaacagga caagaacatt ctttccaaat tggtgacgca gtaaattatc aaggaacgat
     2641 tcatatccca acagacattg caggcggagc agacggtatc acagtaaacg gagtgaaaag
     2701 cgaaacttct ccttacagtg tatttaaatg gggggataca gcaggacaag gactttctta
     2761 tgtcgctgct aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga
     2821 aaatacagat tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa
     2881 taatggccaa gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta
     2941 taacatgtat gtaaatgata gcgcagctgt tgccaatcca ttaacaaata gcgtggattt
     3001 cgtttataac aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     3061 tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa
     3121 agcaacaccg cttgaaggcg cagaatttgc tgctaaaaac gcagaaggaa aatattatgg
     3181 tggattagtt gatacggata aagacggtgt caaagaagca gtatgggtag acgatgtagc
     3241 aaatgcagcg atcctaaaat cagataaaga aggtcacttt gaaatcactc gtttaactga
     3301 aggtgaatat tcattggaag aaacaaaagc gcctgaaaac tatcaaaaat taacgaaaga
     3361 gatctctttc aaagtagaca aagattcata caaagaagaa aaccgaatca cgatcaaaaa
     3421 caatcaaaaa gtttccgtac caatgactgg tagcaacggt tttcaaacgt atgtattaat
     3481 ctcttgcttg ttgctaggag caggggcact ttctgcagtt gtttacttta agaagaaagc
     3541 ctagaaggat caaaaccaat gattatcagg aagcctaaga aaaagcgaat catgggtaaa
     3601 tggatcattg ctttctggtt actttcagct gtaggtgtct tacttttgat gcctgctgaa
     3661 gcgagcgtgg cgaaatacca acaaaatcag cagattgccg caatcgatcg aacaggaacg
     3721 gcagcagaaa cggattcttc tttggatgtt gctaagattg agttaggcga tcctgttggt
     3781 attcttacaa ttccaagtat ttctttaaaa ctaccaattt atgacggaac gtccgacaaa
     3841 attttagaaa atggtgtagg tattacggaa ggaactggtg atattacagg aggaaacggt
     3901 aaaaatcctt taatcgcagg tcatagtgga ctttataaag ataatctttt tgatgattta
     3961 ccttcagtaa aaaagggaga aaagttttat atcaaagttg atggtgagca gcacgcttat
     4021 caaatcgatc ggattgagga agtgcaaaaa gacgaactac aaagaaactt tgtcacttac
     4081 ttagaaccaa atcccaatga agatcgagta acgctaatga catgtacgcc taaggggatc
     4141 aacacacacc gttttctagt gtatgggaag cgagtaacct ttacaaaatc agaattaaaa
     4201 gatgaagaaa ataagaaaca aaaattatca tggaaatggc ttttaggaag tactgttttt
     4261 ctttcagtca tgatcattgg ttcgctattt gtttataaaa agaaaaaata aaggagggta
     4321 accaacatga gaaatagaaa atggctattt gtttgtgcag ccgctctttt tactttcact
     4381 gcctacacac agttgacggt tgcagacagt caagaaagca cggatagcac agaacaaaca
     4441 atccaaacgt cagatagcag cgcgattcaa agcagcgatg aaaccgtcga ttcgtcaaca
     4501 gttgattctt cagaagaacc taaaattgct gaagacgatc aaatagtcaa acagaccatt
     4561 cacgttcaaa aaattatttc ttccgaacaa ttagacaacc aggggcaaaa gcttgaaaaa
     4621 caagaaggag taaatggcgc gaagttttct gtttatgatg tatcggacat tttacaaaag
     4681 atggatgtta aggatctcac tactgaccaa attgaatccc aattaaaaga tcgagtgaaa
     4741 aaactatcca gtgatcaact gaagctaatt tctaatggag aaacaaagac aattgatcaa
     4801 caaacaggtg tctttgaatt ctcggtagaa gtacaggcaa atcagaaaca agcttattat
     4861 attgtcaatg aatcaagtcc tgaaaacatt tctaatagcg aggatatttt gctattaaca
     4921 cctgtttctg ataaaaatgg tgaattctta aaggatgtgt ggatttatcc taaatcagaa
     4981 gcttcgcaac ctaaagaaga agtaaaaaaa atcgtatcta ctggcgtaaa gaaaaacttc
     5041 tttgagaatt gttgggattt tgttactcac ttgttttcta gagattaagg aagaaataag
     5101 atgaaactga tcaaattatt attattcaat ctttcttgtt taaccgcagt ctttttgtac
     5161 tgcggtttat tattttctca actatctatt ttgccaagct accaactata cctaatgagg
     5221 attttaaaca taccagctgc ttccatgccg ctagatatta tggaaaagct cattaggaat
     5281 ccattattta ctacgatcaa tttgatcgtt attgtcggaa tttcactagt catttataaa
     5341 aaagagggga tgaagggatg aattttaaac actcgtggac aagacggtta aaatatgttt
     5401 ttcccataat gatgatgtta ggtacattgt ttgggttaaa aacagccaat gtctttgctg
     5461 aaaaagttat tgtcgatccg tcaaatccaa tccagaacgt aaagaataat tttattattc
     5521 caaaagatgt tcctaatgca aaaacaaata ttactgtaaa cggagcgtca aaagtttaca
     5581 aatatcatgt tgatgccaac aaaggtggtt ttacagacga ctacgtagga ttagtggagg
     5641 gaaacaaaaa tatacgctat ccttctttcc aaaaggaata cggtgaaaca aaccttgttt
     5701 tagaaggcgt aacaacaaac accaaagtca atattgacta tggaaaaatc ggtacgtata
     5761 acgggaaaaa agtcaatatc aagctagttt tatctaatat tcacttatac tcggatacgt
     5821 tgccgtggaa tctactagat aataactata caaaaactca ttttcgagac gatggctata
     5881 aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac agtactttgg atctcagaca
     5941 atcttttctc aggaattgtt taccattcaa cacaaatgaa tgtgcagcta gtagccacat
     6001 atgaggacgg tagccccgtt caattttcag gagatacatt tatcagtttt aactctttga
     6061 accctgcagg aggaaaaagc acggatctta aaggagaata tacccactat gacaaaatga
     6121 atacaacaga ttggtatgtc agaaaagata cggttctttc agaatttaaa tctttctaca
     6181 acaatctgaa cgtggtaggt ggccacccag gaggaagctc taagctgaca caagcggata
     6241 atgatttcaa taatttgcat gataaattag gagatccgaa gtttggtcaa ggtactgtgt
     6301 cctttaaaat atctgaagca aatccgacgt ttgtgattgg ttcttctaat gttcaaacct
     6361 ggtttacttt atcttctgcg acaatcttta gtgttgttcc tgatcaacct gaaaaaacag
     6421 gggttgataa aaatggaaat aacgtgaacg ataacatgtt gcaagtagga gacacgattc
     6481 aatatcgaat caaacagaag gtgaatcgat tgggagtgga tttactagcg gtttacgatc
     6541 gatttgagtt aatcgataat ttaccaaaag aagtagatta cgtcgatgca cacgttgaaa
     6601 gtggtacaaa taaaaaattc gatgtatctg gtgaagtgac ctatgacaaa acaaaacacc
     6661 aggtaaaata tgcagcgaaa gctgatacat taaaaaatcg aatgaagtat aacggcgaaa
     6721 cttacgaatt agtgatcaat gtaaaagtca atgaattggc caatcaaaat agtgtagcca
     6781 aaaatcaggg aacgtcgatc attaataaag tagaaaagga tacaaatatc attaccgttt
     6841 atttccctaa aattccagtg aaagaagtac aacaaaatgg aaaagatgta aacggacgaa
     6901 atgacggtaa gaagggtacg ccgacagctc ctttaaacgc aggatctgaa gtacaatatc
     6961 tagtcacaca aaaatggcac acaaaaggcg tagacgctgc gtcagatcac tacaaacaat
     7021 ttagtattca agatccaatt gaagctcgtt tgacatataa agaagtttct gcgcaagtaa
     7081 tagataagag taaaggaaaa gatattactt ctgaaggcac actaacctat gatagcaatt
     7141 taagaacgct gaagtgggaa gcttccgctg acttcttaag taacaatctt ttagatggac
     7201 gagaaattca gctgatattt acagctaaaa ctccattaca atcagaaaaa aatattgata
     7261 accaagccgt agtagcagta gataatgttt ctaataaaac aaatgttgtg acaattgggg
     7321 ttgatcctaa cttgccacag gtcattgttc ctaaaacagg ttctacacat ttagtaacga
     7381 tttcagcggt cagcttacta ttacttgtgt tagcaacttt tagttatgcg gttctgagat
     7441 acatttaaaa taaaaaaacg aaagaatgaa taaaatgaaa aagttaataa tagtagttac
     7501 ttccttgatt gccatgattg ttattttagt cggatgtagt aatgaaaaaa ataaacaaac
     7561 taatcaagat aatggcgtat tgaaatcagg aactatgtgg aaagaagaag taggtggtct
     7621 tgtttataat ttgaaaatta tagacgagac tacctgggaa tactctgaga gtgtatggca
     7681 tccagatcct gtacaaataa cagtcaagag gcaaaaagac tataaaggtt tagaaagata
     7741 caaaatagta gattcagctg gtgttaggga atttataaac aaatctgatt ctttatttat
     7801 tgttgttccc tatgagaaga atggcgtaaa aaaaataatt tttcttggat ctagcaagga
     7861 tgaaaagcga acgaaagaga aattgattca cgatggatca caatccaata aatataagtt
     7921 acaaaagact agcgagtgat tgctagtctt tttatttgtc ctgaaggagg aatcacagtg
     7981 gcaaaagcta aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg ccgcggtgta
     8041 tctgaaacac agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga
     8101 aaagacaagg acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat
     8161 gaaattatta aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt
     8221 ttttaacaca accgaaagta gtagctgcgg ttgatatttt tagagcatta caaactgccg
     8281 caactagttt aaaaaacaat ctgatgattt ttgctgctgc tgcggccgtt gcttccattg
     8341 ttattggggg tcttttcttc ctatttggac gaagaggagc tgaaaatggg aagatgcacg
     8401 taggctatgt attgatgggg gtaggagtta ttttagcagc agcttctatt gtggtttcgt
     8461 tatttggatt gtttggacaa acacctcaag gattataaga aagtaaaaaa gtaggagtaa
     8521 gaagtagtcg atacttttta ctcctttttt atgaaaggag aatactcatg tactttacga
     8581 ttgcaggcaa taccaaaaca gataaagagt taataaaaaa agcaacgaag gaagtaggaa
     8641 agcgtacttc ttttttaccg aaaacaatta aatattctgt cattgaaacg gaagaacagc
     8701 tacatgtgtt agggaaagta ctggaacacg aaagtcgatc ttctgttaag ttgactctca
     8761 cgttacatga cacagaagga aaaatagaga aagctggtac agaatacgtt tttggcccac
     8821 tggattatca aatttcagaa ggtttctatc ctacattgat ggatttaatc cgccttgatg
     8881 tgtttaaaga aacggatgaa actatgagtg tggaagagtc ttatcgaaac gaaaaacttt
     8941 tacaagtttt agaagaagag ttagagagcg accaaagaaa agtgaatcaa gggagtgaag
     9001 tcgaagtaaa tgaagaagta ccatgggaac gaccaatatt tccaaagaag gagtcaaaag
     9061 aaaaagagac agtctatgtt gacgaacaag ctgatattcc gactcagaaa tcgatatttg
     9121 aggaagagaa cgatgtgatg ggtcaattgg aggtagatcc ctttgaagaa acaatggagg
     9181 atgaagagac ggccgaagta gaagttgatc cagttgaagc taatgaagag tcaacatgga
     9241 ctgaagggga ttctcaggaa tatattgaac cacttatcag tgaagaagta gaaaaaaacg
     9301 aaatagagat acaaaaggca actgtatttg attatatttt ggatcaatat ccgccagagc
     9361 atgaatggat gaaagaaaaa aacaaattat ttttaaatga gctttattcg aaatatcaat
     9421 taccccaaac ctatcaagca tttttagaga gtaaagaaga attaatctca cagggtaaag
     9481 gcacactttt agaacagtta gagtctgtcc aatcaacaaa ttggcatgag cttgcacaag
     9541 aagatttatt agccaccttt gaagaacgtc aacaagaaac agatcaagaa attaatgagt
     9601 atgcgacgga acagtcgtct aattgggaga aagcaaaaga ggaactaaat aaagaagaac
     9661 agcgagtgat tgaagaagaa gtacaaaaaa ttaaagagcg atacgaaaag aaacgcgaca
     9721 atgcgtattc cttgtgtgta gcaaagatca ataattttac tcagacgaat caagaagcgt
     9781 tatcgaaaga aaaagaaaac ttgttaaaag aacgtattaa agaacgaaaa gaagaatttt
     9841 accgaggttt gcgtacagat aagctaaaga ttagcaaaga attaaatgaa caactgaata
     9901 agctatatca aacaacgact gaagttctta tggaaaaaca cgaacagatc cagcaagaat
     9961 tacaagtaca aattccaaaa tggaaaaaag agtatgaaat tcaacaacaa gaattgaaag
    10021 aacaagaaga aaaacaacgg caaaaagaaa aagagcgcga acaattagaa ctaaaaaaac
    10081 gtgaacagga tcttgcggaa caacaaatgg caatagaaaa acaacggtta aaccaagaaa
    10141 aggaaaaaga agctgctcgt aaggaacgtc aagaacaaat ggatacattg cgacaagctc
    10201 agttaaatta tctagcacaa cctcaaccaa cgccttcagt agctgctgtt caaaccccaa
    10261 tgaacaacca aacaaatcta actccttcac cacagcgttc ctttaacgga tggatggtgg
    10321 gatgtattgc aagtttaagc ttgcttctag gtggagggac agtatttgct tttaatcata
    10381 tcaatcaatt ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    10441 aagaacaagc aagaaaagag tatgaacaac aattggaaga attattgaat cgtaccaatc
    10501 agtcaacaac agagtcaagt atggaaaata gcaaagagag tgatcctagt aaggctaaag
    10561 aaggatcaag cgattcaact gtacaatcaa acacttccga aagttcagaa tcaaatgagg
    10621 tgaaataatg cgtgttagag aaacaacgag acgaaaagac aaacgattca atttagaaaa
    10681 gaataggtat ctttctcatt tctatcgcta tactccttca ggagaagagc taccagaaaa
    10741 acatgacaaa cgtatttctc aaaagatttt ttatagtgga ttagcttatc ttcccttaaa
    10801 ctatgttgtt acaagaatac cagaagcatt tcatcgattt gagattattc caggaagttt
    10861 acaaggagtg aaaatttttc agttacaaga aatgccgcag ttattccatt ttgattcaac
    10921 tggtttttgg aatcctttga ccccaacaac aagtttaggc acacgtttag ttgtttctac
    10981 gatttgtgtt gttccagcta tacttatggg attgaaaatg gacatgtggt ttcgtcctct
    11041 agcagatggg caaaaaggag ataaccgatt aaaaacgtta aaggaaatcc aacaaattta
    11101 tccgcaaata ccagaatcaa agggaggttt tcctggttat ggcggaatac caattactca
    11161 ttataaaaag tactggtata ttcagacgga tacagtaaat acttgtatta ttggtacttc
    11221 ccgttcaggg aaaggacaga tagaagtttt agcaaccatt gacaatttgt ctcgtgcaga
    11281 aagacaaagt tcaatggttg ttaatgatcc aaaaacagaa ttatatgttg ctagtaaaga
    11341 tgaactagaa gctagagggt atgatgtgta tgcatttaat atcaccgatc cactgcaatc
    11401 aatgagtgat aatccattgg cactcatcgt gaaatactgg aagcgaggag acatagatac
    11461 tgcaacgcag ttaaccaata cttttacgaa tacgatttat tatgatgcca atgcttcaga
    11521 caacaaatac tttaacgaaa atgcgcagaa agctgtaaat gcgcttattt ttgcgttgtt
    11581 agaacaagcc gatcaaacag gggattactc caaattaaca cccaataatc ttgttgagct
    11641 tctttctgaa attggcggtt ttaattatca agatcctgag aatgattaca aacaactcaa
    11701 tgccttagat gaattcatga atcagttacc acctggaaac atagcaaaaa aacaatatgg
    11761 ggcaacaaaa attggttctg ataaagcaaa agggaatatt ttatcgacgg caatcacagg
    11821 attgaatcca tttaccctaa cgaaaattgc gaaaatgact tctcaatcaa ccattgatta
    11881 taagtccatt ggttttccga agtatttgga tctcaaacta cacgagagtc tgttaaatca
    11941 gcgaattgtt gtagagtttt atcatcaagg gaaaaaattt catgaggaac gtgtcaaagt
    12001 aggttataga ggtttttgtg aaatcaactt tgattgtcat ttagagcagg gagatcatgt
    12061 ttgcttaaaa tacgatcatc aaggaaaaga atatgttgct tggtatgagt tttctcaaag
    12121 agtattgaaa gacgaagagg gaaatatcgt ttatcgaaaa aaacgagaag aaacccattt
    12181 accagaatta gaaaaagaag ctgttctaac attagatgag gataaaagta acttattagt
    12241 agagtctatt cggatgcact ataccgataa accaatggct atttttatgc tcacacctga
    12301 ttatgatcca tctaaccata tcattgttag tatttttctt agtcagttgt ataaagaatt
    12361 atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac cgtaggattc attttatttt
    12421 agatgaattt gggaatatgc caccaatgga taatatggaa ggaatcatga cagtcacagc
    12481 tggtagaaac atgttgtggg atctgtttat tcaatcctat cagcaacttt atgcgaagta
    12541 tggggaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    12601 gacagacgat aataccatcg aagaattttc caaaaaagtg gggaataaga cggttgaaca
    12661 agagaacagt accaccaatt tattaggtat gaatactcat atcaatcgca atgttgattc
    12721 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    12781 cgctccttta aaacgacaag atttgaaagg acataaaata cggccgtatc ctatttttaa
    12841 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    12901 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    12961 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    13021 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    13081 aaatgagaca gcccaaaaat tattaattca attttacgaa gaacaagcaa cggatcgtct
    13141 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    13201 gttgttattg gaagctgaaa aggcggtgaa agaatgatca ttggaggaat tttttctggc
    13261 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    13321 aaagtcttac aagattatgg taattatctg cagtatcaag atgtatttag ttggatttgg
    13381 aacacttttc tgtggggatt gattaaagga ttatataatc tcaatacttt actagaaaaa
    13441 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgcacta
    13501 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    13561 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    13621 tttatggcca tgatcttgat ttttggtggg agttctttaa tcgatgaagg gttaaatatc
    13681 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    13741 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    13801 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    13861 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    13921 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgattggaa
    13981 ctagatgata atgggaaaga aattcctgtt caaattagtg acgaaggctt agcaaaatat
    14041 gtctatactt cagggtatcg tcgatacagt agtaaaccag gtattatctt agctggtgaa
    14101 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    14161 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggccaa
    14221 cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc gtttacgatt
    14281 ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca tgcggcagga
    14341 aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc gctattcaaa
    14401 ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa cggcagtaat
    14461 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    14521 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    14581 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    14641 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    14701 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    14761 gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg cacgagagct
    14821 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    14881 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt taatgcagca
    14941 gtaaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    15001 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    15061 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    15121 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    15181 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    15241 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    15301 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    15361 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    15421 gatagtcgtt tgaatcaatt caaggagaat gatcaaagct ttgttcacca atcaactgca
    15481 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    15541 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    15601 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    15661 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    15721 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    15781 ggccttttat ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    15841 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    15901 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    15961 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    16021 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    16081 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    16141 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    16201 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    16261 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    16321 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    16381 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    16441 aatcgtgcaa aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa
    16501 tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg
    16561 agcagaacaa gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa
    16621 ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    16681 actctacaaa aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta
    16741 ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg
    16801 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat
    16861 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    16921 aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa
    16981 tcgggaaata gaaattttaa aatccaaagg gtatgattta gattttttaa aacaagtgat
    17041 gccacaaggt gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg
    17101 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    17161 gaacaatcct catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat
    17221 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc
    17281 gactgaccga aataaaagcg cagaagaata cctggaacta gcggaatatg ctcgtaaatt
    17341 gacttctaat ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac
    17401 gattcaagaa gtagatgaac gagcgcagga actgatcgaa aatctaaaag ggatggatta
    17461 tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt
    17521 cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat
    17581 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg
    17641 ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt
    17701 gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    17761 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    17821 ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg
    17881 aatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata
    17941 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt
    18001 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    18061 agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    18121 tacttcctca aattcgcgtt caaatacgga gacgcaagtc acaggtaaag atccagaagc
    18181 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    18241 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    18301 gtatggcatg atttttgatg ggcatagtac gattcgacac cttgaccaac aacagttggt
    18361 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    18421 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgac
//
LOCUS       DASJRQ010000056        17817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJRQ010000056 DASJRQ010000000
VERSION     DASJRQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.056"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="U4A80_001751"
     CDS             248..1021
                     /locus_tag="U4A80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1975375.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="U4A80_001752"
     CDS             1018..1785
                     /locus_tag="U4A80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1975376.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="U4A80_001753"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="U4A80_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1975377.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="U4A80_001754"
     CDS             3750..4070
                     /locus_tag="U4A80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1975378.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="U4A80_001755"
     CDS             4070..4420
                     /locus_tag="U4A80_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1975379.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="U4A80_001756"
     CDS             4587..5525
                     /locus_tag="U4A80_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1975380.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="U4A80_001757"
     CDS             5540..6370
                     /locus_tag="U4A80_001757"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1975381.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="U4A80_001758"
     CDS             6414..7439
                     /locus_tag="U4A80_001758"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1975382.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="U4A80_001759"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="U4A80_001759"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1975383.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="U4A80_001760"
     CDS             8517..8957
                     /locus_tag="U4A80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1975384.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="U4A80_001761"
     CDS             8960..9562
                     /locus_tag="U4A80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1975385.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9630..10736)
                     /locus_tag="U4A80_001762"
     CDS             complement(9630..10736)
                     /locus_tag="U4A80_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1975386.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10946..11947
                     /gene="ccpA"
                     /locus_tag="U4A80_001763"
     CDS             10946..11947
                     /gene="ccpA"
                     /locus_tag="U4A80_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1975387.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(12283..14628)
                     /locus_tag="U4A80_001764"
     CDS             complement(12283..14628)
                     /locus_tag="U4A80_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1975388.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            14907..15809
                     /locus_tag="U4A80_001765"
     CDS             14907..15809
                     /locus_tag="U4A80_001765"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1975389.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            16022..17797
                     /locus_tag="U4A80_001766"
     CDS             16022..17797
                     /locus_tag="U4A80_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1975390.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         5693 a         3171 c         3696 g         5257 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtctgcacta cttgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaataaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag gcaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcaga cgttagaagc agaaatcaaa
     2341 gaattagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaacctttct gtgtctttgg taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatggtgaa atcacgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagg ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatccaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaatcattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagtg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc ggctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gtcatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc taattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ccatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggtgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc gaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gagtaggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactactcaa gcagcgatgg aactagccca aataatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat gggaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag tcaaaaaagc agttattcca gcggcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggaatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac cgtttgtcgt tatgctaggc gatgatatca tggaagatga
     7861 agtaccattg acacagcaat taatcgatga ttacgaacgt actcatgctt ctacgatcgc
     7921 cgttatgcaa gttcctcatg atgaaacttc aaaatacgga attatcaatc caggagaagt
     7981 attggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttggacgtta cttattgact ccagaaattt ttgatgtatt
     8101 gtccgagcaa caaccaggag ccggaaatga aatccaacta actgacgcaa tcgatacatt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttacg atgtcggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtcta acacatccag aagtaggtgc
     8281 acctttacgt gagtatatcc tgtctttagg taaaaaatta gaagcagaag gaagcaagat
     8341 cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aacccttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttta
     8581 tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag actgtagcag
     8641 aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct agacaagtag
     8701 aaggcttgct ggtcaaaagt aatgaactgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcagaa cttaatcatt
     8821 ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg
     8881 ttgccggaaa agtcggtggg acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataaaaa tggctaaaaa aggtggattt ttattaggtg cagtagtcgg
     9001 aggaacagcc gcagcaatcg ccgcgttatt gttagcacca aaatctggta aagaacttcg
     9061 ggatgaatta gctgcccaag cggatgattt gatggatatg gcttcagatt acacagatat
     9121 ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc aagatctttc
     9181 tcaacaagca ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat cagcagatga
     9241 agcaggaagt gaatctgacg atatcttgtt aaatcttaaa gaacaatcag ctgacttatc
     9301 taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat taggaagtat
     9361 tgctaaagat acttcagaag atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg
     9421 agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag atacaatgac
     9541 agattcatct acaaatcact aaaatcacta aaatcacaaa tttatagaat aaaaaggtgg
     9601 gagcggacta gctctcacct tttttactct tatttttcta aaactagtaa ttcttttggt
     9661 gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc gatacgtacc
     9721 cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc ttcttcgatt
     9781 actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc gatcccgtga
     9841 cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt tcgtgcgatt
     9901 tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc aagctgtgct
     9961 tcaagagtga tgttgtatat tttttcttgg aagtcggttg gtttttgata agcaattgta
    10021 cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa cacaagttca
    10081 ttcggtgaaa tcttcgtgtt tcctggtgta ccatgagggc ttgcagcatt tgctcctgca
    10141 aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg gtattcgatt
    10201 tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc aatctcaaat
    10261 gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag agttttgatc
    10321 agttgtagac gttggatgat ggaagctaga tcagtgtaag aatccgttcc cgtcaagata
    10381 tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc tacattgcca
    10441 ggctgaccat agcgagcacg aagttctttt ccgatgatct cccaagggtt ttcgctgtct
    10501 aaatagccac ggacatcata cgcccaacta ctgttttgag catcttcaac ttccaaagca
    10561 ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac acgctcatga
    10621 ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat ataagccaat
    10681 tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt attcatttgg
    10741 caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt attaaaagac
    10801 caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata aaacggttgc
    10861 aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt cagaaaacat
    10921 ataaaatatg aataggagaa cacaaatgga aaagcaaaca atcactatct atgatgtagc
    10981 gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca atccaaacgt
    11041 caaaccagca acaagaaaaa aagtcttgga agttatcgat cgcttggatt atcgtccaaa
    11101 cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa tcatccctga
    11161 tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg caacaatgta
    11221 caaatacaat attatcttag caaattcaga tggtaacgac caaaaagaag tcaatgtatt
    11281 gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc atatcacaga
    11341 cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg gctctattga
    11401 tccagatgaa caagtaggta gcgtaaacat tgattatgta tcagcaacaa aagatgcagt
    11461 aaacaaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg cgttgatcga
    11521 tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg aaaataacct
    11581 accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg gtctatctct
    11641 agtcgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg atgaattagc
    11701 catcggtatt ttggatggtt tattagaccg tggcgtgaaa gtaccggaag aattcgaaat
    11761 catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa caagtatcac
    11821 acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa tgatgaataa
    11881 agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa aaggatctac
    11941 aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact ctgcgttaca
    12001 aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc gtgattcgga
    12061 gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt tgtttaatcc
    12121 tttttttttg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac
    12181 aatccgtgta tagatgctgc attatgttca ccaccatcaa aaaaataagg gactgtgaca
    12241 atactattgt cacagtccct tattactgtg tattctatta aatcaatcat ctttctcatc
    12301 tgattctttg gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt
    12361 atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt
    12421 cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac
    12481 ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa
    12541 ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag
    12601 attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc
    12661 gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg
    12721 gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa
    12781 gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac tgcgcatcat
    12841 gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt
    12901 ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac
    12961 gccgccgttt gctaaagcct gaaatccatt ggtctgttga agcgtcgtga catctactgt
    13021 acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa
    13081 gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg
    13141 ataagccggg atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga
    13201 tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt
    13261 tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata
    13321 aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt
    13381 ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc
    13441 catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata
    13501 attttctacg ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt
    13561 atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga
    13621 atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc
    13681 attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag
    13741 aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc
    13801 ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc
    13861 ttttagcgca cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc
    13921 gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttcctcttgc
    13981 agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt
    14041 caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat
    14101 gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa
    14161 ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc
    14221 tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct
    14281 gataattgca gttttcaata aaggtgaaat tttatcagat gaaatagtcg tgcgcatcag
    14341 atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt
    14401 aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag
    14461 ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac
    14521 agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt
    14581 ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc
    14641 aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct
    14701 gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tcttttttat
    14761 aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa
    14821 gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca
    14881 attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa
    14941 agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg acttttatga
    15001 tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg
    15061 ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat gccccttata
    15121 tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga
    15181 gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg
    15241 ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag
    15301 ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg
    15361 aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttgaatgaaa
    15421 aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag
    15481 acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac
    15541 tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg
    15601 gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca
    15661 atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa gcttacgcac
    15721 catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa gtgtttcctg
    15781 agttattagc agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac
    15841 caggcacatt gccttgtcct tttttgttta agaaacaaag gagtgctttt tgcagtcttt
    15901 ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacagggta tactggtaga
    15961 aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa
    16021 gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat
    16081 tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta
    16141 tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat
    16201 tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta
    16261 cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt tgttcggcat
    16321 gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg tgaagaacaa
    16381 gtatcttctt ttaagtttct atttttattt tttgttttct ccgattagtt ttacacatga
    16441 atattccact cgaacgtatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc
    16501 ttgtttacta gctatttatt accgtaaata tagttccagc aaaaaaatgc tgccgtggtt
    16561 tttagggcta gctgtttctt ttcctttctt ttggttcatt cgcgaagatt ccatctggct
    16621 tcttcctttt actgtagtcg cgttaggcat cagtttcgtc cagatcttgc taggacaaga
    16681 cttttctttc aaagaagcaa agaagttaaa agaacgacca tttctccttt cgaaacaaga
    16741 atggctgaaa ctttcattgt ttttactccc tctagtcggt ttctccatcg tctatcagtg
    16801 gatcgaatat aaaaacgaga caacatatag tattgccgcc atcaatgacc gttcgagtgg
    16861 cgaatttgga cgtttgatga aacagctgat ccgaatagat gacgaaacga acttaaataa
    16921 agaaaacagt gagatatggg tctcgcatgc tgcattaaat aaagcattag acgcatctcc
    16981 tacatttcat gctgtttcta ctcgtattga cgaattgtat acgactcatg cgtggaccca
    17041 aggtggaaaa gtaaaagaat taccaggaga tatcattttt tgggcattgc gagatgtggt
    17101 aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt
    17161 caatcaagaa ttagaagaag cctatcaaaa gggaactatc aaaaagaaaa aagaattcta
    17221 tctgatggga tcaggggatg gaaagacgat gaatgatctg ccaatcgtat ttgcttttct
    17281 gaaatcagga tatgcctatg catggcgata caaagatttc catcaagggg ggaattatag
    17341 tttcggtacg caagaacaag tcgaacaggc ccaagaatta ttgaatcttc cactaatgga
    17401 taattggatc gatagcagtg atccgagaac acctgatttc cagttgacaa aaacagcaaa
    17461 agtcgcgaat gtcatgataa aaatttacca agttctggcc attcccgtgc tgattgcttc
    17521 aatagcaggg atattactta tgatactagg aaccgcattt gcaaaagctg atcgtgccta
    17581 ttatagttcc tctttgattg tagtcaccgg attaatactg acacaattgg tttttctttt
    17641 cggcgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt taagtgtcta
    17701 cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag
    17761 taaattgaat ctgaaaaaag ctttatcttc caaataaaaa catactaaga ttagagg
//
LOCUS       DASJRQ010000057        16950 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJRQ010000057 DASJRQ010000000
VERSION     DASJRQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.057"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..604)
                     /locus_tag="U4A80_001767"
     CDS             complement(<1..604)
                     /locus_tag="U4A80_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1975391.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYE"
     gene            complement(852..1688)
                     /locus_tag="U4A80_001768"
     CDS             complement(852..1688)
                     /locus_tag="U4A80_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1975392.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1699..2628)
                     /locus_tag="U4A80_001769"
     CDS             complement(1699..2628)
                     /locus_tag="U4A80_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1975393.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2820..3173
                     /locus_tag="U4A80_001770"
     CDS             2820..3173
                     /locus_tag="U4A80_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1975394.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3247..3903)
                     /locus_tag="U4A80_001771"
     CDS             complement(3247..3903)
                     /locus_tag="U4A80_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1975395.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4129..6063
                     /locus_tag="U4A80_001772"
     CDS             4129..6063
                     /locus_tag="U4A80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1975396.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6149..7372)
                     /locus_tag="U4A80_001773"
     CDS             complement(6149..7372)
                     /locus_tag="U4A80_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1975397.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7366..9264)
                     /locus_tag="U4A80_001774"
     CDS             complement(7366..9264)
                     /locus_tag="U4A80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1975398.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9258..10376)
                     /locus_tag="U4A80_001775"
     CDS             complement(9258..10376)
                     /locus_tag="U4A80_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1975399.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10459..11292)
                     /locus_tag="U4A80_001776"
     CDS             complement(10459..11292)
                     /locus_tag="U4A80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1975400.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11285..12202)
                     /locus_tag="U4A80_001777"
     CDS             complement(11285..12202)
                     /locus_tag="U4A80_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1975401.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12429..13553)
                     /locus_tag="U4A80_001778"
     CDS             complement(12429..13553)
                     /locus_tag="U4A80_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1975402.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13736..14644
                     /locus_tag="U4A80_001779"
     CDS             13736..14644
                     /locus_tag="U4A80_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975403.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14785..16398
                     /locus_tag="U4A80_001780"
     CDS             14785..16398
                     /locus_tag="U4A80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1975404.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5348 a         3302 c         2804 g         5496 t
ORIGIN      
        1 actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat
       61 ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt
      121 catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg
      181 tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt
      241 tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa
      301 taacagttat tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct
      361 tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta
      421 ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa
      481 cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca
      541 aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta
      601 ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa
      661 catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc
      721 gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca
      781 tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt
      841 attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca
      901 gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg
      961 atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc
     1021 gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag
     1081 aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt
     1141 ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa atagttttca
     1201 tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct
     1261 aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc
     1321 tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc
     1381 gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt
     1441 acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt
     1501 gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga
     1561 gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg
     1621 agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac
     1681 cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag
     1741 tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt
     1801 gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact
     1861 taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa
     1921 taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt
     1981 tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt
     2041 atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt
     2101 ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc
     2161 tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac
     2221 accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga
     2281 attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag
     2341 cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg
     2401 tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga
     2461 gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa
     2521 ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa
     2581 gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct
     2641 ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc
     2701 tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt
     2761 tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa
     2821 tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg
     2881 tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg
     2941 aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag
     3001 tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag
     3061 aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac
     3121 tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt
     3181 ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt
     3241 ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat
     3301 caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt
     3361 caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac
     3421 tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt
     3481 gatttgttct gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac
     3541 gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt
     3601 gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg
     3661 atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc
     3721 acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg
     3781 aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct
     3841 taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt
     3901 cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat
     3961 ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt
     4021 atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa
     4081 aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta
     4141 caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta
     4201 gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact
     4261 ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa
     4321 acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg
     4381 aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta
     4441 gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa
     4501 tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt
     4561 cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact
     4621 ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac
     4681 attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag
     4741 aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta
     4801 gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt
     4861 aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat
     4921 gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc
     4981 gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg
     5041 gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct
     5101 ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct
     5161 gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt
     5221 atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa
     5281 gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat
     5341 acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag
     5401 atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct
     5461 ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa
     5521 aattttttga ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta
     5581 gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc
     5641 atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta
     5701 ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc cgaattgttg
     5761 gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta ttaccaaagc
     5821 aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa
     5881 atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc gccagaaaac
     5941 ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc tagacaagat
     6001 caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga atatgaaaat
     6061 taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt ttttatttta
     6121 tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta ctagtcattc
     6181 tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt accccattat
     6241 ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc gttgtagcat
     6301 atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag ttcttttctg
     6361 ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg atcaccatac
     6421 tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa taacaaatgg
     6481 tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa tcaaaatatt
     6541 tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat gcttggcctc
     6601 tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt ggttttccat
     6661 ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact aaataatctg
     6721 ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca gtttcttcac
     6781 tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta ccttgttgtg
     6841 aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa aaacgctgaa
     6901 gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc gttattttat
     6961 aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta gtgataattt
     7021 gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt tgaaataaag
     7081 gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct tctgtccaac
     7141 caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg aattgatact
     7201 gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc tcgatctcat
     7261 tattccattc tttttccatt aattctttat aacattcatt tatccatttt tctggatgta
     7321 ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc ataattttag
     7381 tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa aatcaatttt
     7441 gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa ccactcctgt
     7501 tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa aagtgttttc
     7561 ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc gtttaatagt
     7621 atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat agttttcctt
     7681 gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa accctgtctc
     7741 atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc agccttcaaa
     7801 aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag aggcaagtac
     7861 ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc cataggtttt
     7921 gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat
     7981 tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg
     8041 actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat ttggccctaa
     8101 ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca aattggcttc
     8161 tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg tccaatactc
     8221 atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg gagataacgt
     8281 actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat tcaggatacc
     8341 tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga ctaacccctt
     8401 gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc aaaagcttga
     8461 aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa
     8521 aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct ggctaatggg
     8581 cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa aaggattata
     8641 ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat aacaattttc
     8701 tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa atttttggat
     8761 aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt catattcaaa
     8821 aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt ttttccataa
     8881 taattgttca tgatgatata acgctaataa cattgcgggc atttctacca tacgctggtc
     8941 tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag cttttattcc
     9001 tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc ccgctcccca
     9061 taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta ccccttgatg
     9121 tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt gatttatctt
     9181 tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat gtttcattgt
     9241 attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg aagtaagcct
     9301 tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc tctatggtat
     9361 gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc atagtgcttt
     9421 tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg tacagaggaa
     9481 acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact ggtatcaata
     9541 ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc gattttctga
     9601 ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt tgtgtaacga
     9661 taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca agaattctca
     9721 ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc aaattctgcg
     9781 atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc tgcatgctgc
     9841 cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata cagtaatggt
     9901 aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca tgctactaaa
     9961 tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt
    10021 cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa
    10081 cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa ttgttcttgt
    10141 tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg ccataaaata
    10201 ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac gtctgtatat
    10261 tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc cgcatctatc
    10321 ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc attcatggat
    10381 atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga actagatggt
    10441 taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct taaatttaga
    10501 ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa ttagcataat
    10561 tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga taattaaggg
    10621 aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt gattccaact
    10681 tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg gtacagcgat
    10741 tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca tcagtgctgt
    10801 aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg caatcgaagg
    10861 gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt aaaataaagg
    10921 aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga acaacagttt
    10981 attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac tggctgtagc
    11041 tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg gataattata
    11101 tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga aattgattcc
    11161 gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata aatatggcat
    11221 caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc tttgtatctt
    11281 ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat ataacagata
    11341 caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt tcaaaacttg
    11401 ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg gaagtcccaa
    11461 agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa actgtggcca
    11521 ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga aatttcgatg
    11581 ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga ccagctaaaa
    11641 ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt gatattcgta
    11701 tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc aaaaatccat
    11761 tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc accgttgatg
    11821 taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac tgtgctgaat
    11881 tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat cctgccatga
    11941 taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata gctagataat
    12001 tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa gcaaacgaga
    12061 taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg accaatccat
    12121 aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt attgtcttgc
    12181 ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc accagttaat
    12241 tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt ttcaaaatca
    12301 cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc tgaataagtg
    12361 gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc atagtatttt
    12421 tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc caatgagtta
    12481 gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt cagcataaat
    12541 gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata aaaacttgat
    12601 cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag tggagccatg
    12661 cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata ccagccgcca
    12721 gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt
    12781 acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc ttctttagta
    12841 ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc tagcatgttg
    12901 ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc agttttttct
    12961 tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc aaaatctaca
    13021 cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa atcactagcc
    13081 caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt ttgatcataa
    13141 aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa agcatcttga
    13201 aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc cggcatatct
    13261 tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga agctacattc
    13321 acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc agcattttca
    13381 acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc tttttttatc
    13441 attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc acttaagctg
    13501 aataataggg taatgtttag caaagctatc accaatttgt atcttttttt catgtctatc
    13561 ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt aacggaatta
    13621 gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt
    13681 tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa ggaaagtggt
    13741 tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa cctattccat
    13801 agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa atgaaagcgc
    13861 taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt cccttttatt
    13921 cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac aaacttttat
    13981 cattatccag ttttattttg atgagcaatt attagcacat attacgggac aatctttttc
    14041 tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt tcaaaattga
    14101 ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt cttttttcat
    14161 actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt gtctgcaaaa
    14221 aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat tgacaatacc
    14281 ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg aattagttga
    14341 tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg aacaattagc
    14401 ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag tgaatggaaa
    14461 aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga
    14521 aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga tccatcattt
    14581 ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc tatccttaaa
    14641 atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc tgtctattca
    14701 aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac atagtacgat
    14761 caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt agctggcggt
    14821 ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat tatctatgaa
    14881 cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg aggtgtgatc
    14941 actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa aaacaacatc
    15001 gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg attaacagtt
    15061 aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat catctcttct
    15121 ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa cggctttgtc
    15181 ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa aaatccacaa
    15241 catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa tttcaagcat
    15301 cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc ttatgatcca
    15361 gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt tcatttagaa
    15421 acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga tggctccgtc
    15481 ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga agtggtgaaa
    15541 gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca gttacaaacc
    15601 aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt agtgactggt
    15661 cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat gctggcagat
    15721 attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt cgcaaaaatc
    15781 tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc atcaggcata
    15841 acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta tccagaagct
    15901 acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt aggtggacaa
    15961 gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc aatcaccgga
    16021 aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc gtattctact
    16081 acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac gaatatcgta
    16141 gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt ctttttagat
    16201 atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa tattccaatg
    16261 aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta cattactttc
    16321 agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg tatcaaagcg
    16381 attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag gagttatttt
    16441 tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt atatagaata
    16501 gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt atagtttaag
    16561 aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa aaaaacatgt
    16621 tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta cgcctaatat
    16681 tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc tttcgaatat
    16741 tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc gactggcttg
    16801 cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta atcatatttt
    16861 tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt gtattaaata
    16921 atttaatttt tttgttccta tttatttaac
//
LOCUS       DASJRQ010000058        16719 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJRQ010000058 DASJRQ010000000
VERSION     DASJRQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.058"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(547..909)
                     /locus_tag="U4A80_001781"
     CDS             complement(547..909)
                     /locus_tag="U4A80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975405.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="U4A80_001782"
     CDS             complement(983..1363)
                     /locus_tag="U4A80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1975406.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1427..2620
                     /locus_tag="U4A80_001783"
     CDS             1427..2620
                     /locus_tag="U4A80_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1975407.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2697..3281)
                     /locus_tag="U4A80_001784"
     CDS             complement(2697..3281)
                     /locus_tag="U4A80_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1975408.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3482..4480
                     /locus_tag="U4A80_001785"
     CDS             3482..4480
                     /locus_tag="U4A80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1975409.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4592..5101
                     /locus_tag="U4A80_001786"
     CDS             4592..5101
                     /locus_tag="U4A80_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1975410.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            5280..6581
                     /locus_tag="U4A80_001787"
     CDS             5280..6581
                     /locus_tag="U4A80_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1975411.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(6695..7015)
                     /locus_tag="U4A80_001788"
     CDS             complement(6695..7015)
                     /locus_tag="U4A80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1975412.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(7163..9397)
                     /locus_tag="U4A80_001789"
     CDS             complement(7163..9397)
                     /locus_tag="U4A80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1975413.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9876..10142
                     /locus_tag="U4A80_001790"
     CDS             9876..10142
                     /locus_tag="U4A80_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1975414.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            10142..11869
                     /gene="ptsP"
                     /locus_tag="U4A80_001791"
     CDS             10142..11869
                     /gene="ptsP"
                     /locus_tag="U4A80_001791"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1975415.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            12033..13079
                     /locus_tag="U4A80_001792"
     CDS             12033..13079
                     /locus_tag="U4A80_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1975416.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            13096..14319
                     /locus_tag="U4A80_001793"
     CDS             13096..14319
                     /locus_tag="U4A80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1975417.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            14408..15295
                     /locus_tag="U4A80_001794"
     CDS             14408..15295
                     /locus_tag="U4A80_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1975418.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            15381..15611
                     /locus_tag="U4A80_001795"
     CDS             15381..15611
                     /locus_tag="U4A80_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1975419.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(15668..16633)
                     /locus_tag="U4A80_001796"
     CDS             complement(15668..16633)
                     /locus_tag="U4A80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1975420.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         5308 a         3043 c         3200 g         5168 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2641 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2701 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2761 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2821 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2881 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2941 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     3001 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     3061 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     3121 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3181 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3241 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3301 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3361 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3421 aacatttttg agaagcattc tcaattaaat tctgctatag tgtgaaaagg gaggaatttt
     3481 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3541 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3601 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3661 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3721 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3781 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3841 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3901 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3961 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     4021 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     4081 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     4141 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4201 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4261 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4321 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4381 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4441 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4501 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4561 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4621 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4681 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4741 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4801 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4861 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4921 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4981 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     5041 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     5101 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtggctct
     5161 ataatttcta ggactgatag aaaatcattc tatcggtccc ttttgagcat gaaaaaacaa
     5221 aacatacaga agctccgtta gaattgaatc accacaacac aatcaaagga gcgttctgta
     5281 tgcctgatct tcatcatatc aaactactac ttggaatcaa agataaaaac attttcatca
     5341 caaatgtgga atcgaagagc attaagaata tcaagagtct tgttgtttct gcgacgttaa
     5401 gtaaagaaat tcgtcgctgt cctttgtgta aacagatgaa tcatgaaggc atgatcgtta
     5461 agaacggaaa gaaaaaatcg ctcattcaac ttaataaatg tgcgaaccaa ctgacttatc
     5521 ttgctttagc gaaacaacgc taccactgtc gtggctgcca cacctatttt acggcgaata
     5581 cctacattgt cgatagaaac tgctttatcg ctaaacaagt gcgctataaa attttagaag
     5641 agctaactga aaaacaagca atgacgacaa ttgctaagca ttgcggcgtt tcttggtcga
     5701 ccgtttctcg gacactcgct tctctgatcc ccatgacaaa agtaaaaaga aattggttac
     5761 cgcgttgttt attaatggat gaatttcgtt cactgaaaaa tcaagtcggt ccctactcct
     5821 tttcttgtat ggatggcgat actggaaaat tactggatat tcttccttca cggaagaaaa
     5881 aggctctcgt ttcttatttt atgcaatttg aacgacgcgc acgattgaag gtaaaggtca
     5941 tcgtgacgga catgaatgtt tcttatgcct ctctgataaa ggaatgtttt ccaaaggcaa
     6001 aactcgttgt ggatcgtttt cacattgtca aacatctcat tcgtaaattt gaggatattc
     6061 gcgtacgaat catgaaaagc tttgatcgaa acgatccaac acaagccaaa tattatcgac
     6121 aattaaaggc gttgtctcgg cttctgatca aacgacagga caagcttgtt tatgacaaat
     6181 ggaccaaatg gcgcaatttt ggttgggcgt atctcaccga aagtgaagtg gtggatcgtc
     6241 tgctttcgat ctctgacgaa ttacgaatcg cctattttta ctatcaagaa attcttcaag
     6301 cgtttcacga caaagaggcg gacactttct ttaaacttgt aagaacgatg cccaattccg
     6361 ttccaaaaga actgcattct ataaaaaaag cttttataaa ctatgaatcc ggcattcgtt
     6421 tagccctcga attgccttat tcaaatgcca aaatagaaaa tctgcatacc cacatcaaag
     6481 cattgaaacg agtcgcttat ggctttcgaa gctttcggaa aatgaagacc agaattttct
     6541 tactaaataa tctgattacc tatgaatcaa aaaacatcta aagtagaaaa ggaattctac
     6601 cttagatgtt cttatctcac tctatcagtc ctatttgaca aagagcctct ttacttttgt
     6661 cccacgctct ttaattatta tgttcaaaat ttgtttacac ggctaatgta gcgcctaatt
     6721 catgttttgg aatcgagatt tcaacaaggc ctgccaattc gattactttg atttcatcct
     6781 cgtggtaaac ggcgtctttg attttatcta agaaataatt tttagcatct tcgatttctt
     6841 tcggctggat tttacgtttg tcattgatca gcagaatttc attatgttga ggggcttcag
     6901 tgtagacaat agtggtattt ccggctgtgt agaccttgac tagatgggca tcagtgtttt
     6961 caagctggtt taatacaaga cgggaatggc tgtttgttac atttaccaat ttcatggcgc
     7021 tcacctcttt tatgtatttt taattgcttg tttctcttat tataatttct tttttataaa
     7081 atgaacagta ttttgacttt tctgaaaaaa gaatgagacg gcatttctca aagaaatacc
     7141 gtctcacgct ataaaactgg atttattcag ccgtttcttc ggctgattct ttagccaaac
     7201 gttctggttt agaagtaacg atgatttttc catctttatc cagttttgct ttcaactcat
     7261 ggatagaagg gtgatcaaga taaaattcag cgataccatc ttcgatttgt tcttggatcg
     7321 tgcgtcgaag aggacgagca cccatagatg gatcatagcc taagtcaact aatttttctt
     7381 tgacattcgt aggaacttcg atgtgaagtt gctgtgctgc cagcatttgg ttgacatcgt
     7441 ctagcattag tgtgacgatt gtcatcaagt tttcttttga taatgcactg aattcgatga
     7501 tcccgtcaaa acggttcaga aattctggtg taaagtagtt gtttaattgg ttcaatacag
     7561 aacgagtaac accttcgcgt gccgcaccaa aaccaacgtt tgcttctact ttaccagttc
     7621 cggcattact tgtcataatg atgatcgtat ctttaaagct gaccgttctt ccttgtgcat
     7681 ctgtcaatcg accatcatct aagatttgca agaacatatg caaaacatct ggatgagctt
     7741 tttctacttc atctaacaag acaagactgt aaggatttct ccgtactttt tctgttaatt
     7801 gaccagcttc ttcgtatccg acataacctg gaggagaacc aatcaattta gagacactat
     7861 gtttttccat atattcagac atatcgaaac gaatcattga atcttgtgaa ccgaatagtt
     7921 cgtaggcaag ttgtttcgca agctctgttt tacctacacc agttggtcca acaaataaga
     7981 aggagccgat tggacgattc tgtttgttta atccaacacg gtttcggcga atcgctttcg
     8041 ctactcgatc tacagcgtta tcttgtccga tgacatgagc tttcaagtca tctgctaagt
     8101 ttttcagttg tgtctgttct ttttctttta attcacccac tggaatacct gtccgttgtt
     8161 caacgatttt ttccatatct ttttcggtaa tcacaggtgt ttcctcttca gtgagctgac
     8221 gttctttcat tttttgaagt ttattgatct gatcgcgata ataagctgct ttttcgaaat
     8281 cttcttctcg agaagctaaa actttttgtt cttctgcttc ttgcagtttt ttctcgattg
     8341 ttttaggatc aacgatttgg atcgtcaagt ttttcttaga acctgtttca tctagcaaat
     8401 cgatggcttt atccggcaag aagcgatctt ggacgtaacg attggataaa gttgcggctg
     8461 cttcgatggc ctcatcagta tattttacat gatgatagtc ttcataacgt ttttgcaaac
     8521 ctttcaaaat actgatcgtt tcttcgacag ttggttcatc tacccgtaca ggttgcatac
     8581 gacgttctaa agcagcgtct ttttcgatga tgcggtactc atttaaggta gtagcaccaa
     8641 ccatttgcaa ttctcctcga gctaaagctg gttttaaaat attaccagca tccatatttc
     8701 catcaccagc agaaccagca ccgactattt cgtgaacttc atctatgaat aggatcacat
     8761 tttctgcttg acggatctct tcaatcaatt tttgcatacg ttcttcaaat tgtcctcgta
     8821 taccagtacc ttggactaaa gatacgacat ctaaacgaat gacttctttg tccattaatt
     8881 tttgcggtac atcaccatct acgatttttt gtgctaaacc ttcaacaact gctgttttac
     8941 caacacctgg ttctccaatc aagacaggat tgttcttggt tcggcgattt aaaatttcaa
     9001 tgactcgttt gatttcgtca tcgcgtccga tcacaggatc gatatcacct tgtcgtgctt
     9061 gttctgtgat attgattccg tattctccca ataaaccttc tgtattgcct tgaggtggct
     9121 gaccaccatt gaagttgttg tttccgccaa attgtgttgg tggtgtttgc tcataatttc
     9181 cttgctgttg catttggcga gacatggaac ggaaaagatc gtccaaactg ccaaatccaa
     9241 atggatcatt ttgggccata ttagtcagac ctccattttg ttgatttttg attttttgat
     9301 aacagctctg acagtaatcg agttgtgtcc gctgaccatt gacagtggca tacaagtgaa
     9361 tcgttgcctc attcttgccg cagttttgac aaagcatgaa tcattcacgt cctttcacaa
     9421 aaatgggtac atccatattt tacatctcgt ttttcatgag gtaaaagaat aacccctagt
     9481 tttttaagag atttatgagg tagaaaaggt gaaatctcta ttgaccattc ctgacctttg
     9541 actcttatta tactaaaaat aaaaagggaa gcaagtaatt cgtttcatat gaaaacaaag
     9601 agggtcaaag gaaagaatga taaacgtcag gagaagagaa agcgacaaga aaaaggaaat
     9661 aaacacgttt gttataaaat gtgaagaaaa ggatgaaaaa gaatccggaa taatttgaat
     9721 acttttatct gaaattgaat gaaaactgtt gtatctaacg aaaaaaaatg ttaatcttta
     9781 cagatgaaag ggtggcattt gttaggattc tctgaattac taaaaaagaa aaccctcaca
     9841 taaaaacaac actcacttaa aggagaccga ttaatatgga aaagaaagaa tttcacgtag
     9901 tagcagaaac tgggatccac gctcgtccag ctacactatt agtacaaaca gcaagcaaat
     9961 ttaactctga tgtaaactta gagtataaag gtaaatcagt aaacttgaaa tcaatcatgg
    10021 gcgttatgtc tttaggtgta ggtcaaggtt ctgatgttac tattactgca gaaggtgctg
    10081 acgaagctga tgcaatggca gcaatcgttg aaacaatgaa gaaagaaggc ttatctgaat
    10141 aatggttgaa atgctaaaag ggattgccgc tagtgacggt gtagccgttg caaaagctta
    10201 cctgctagtt caaccggatt taacattcag taaggcaaca gtagaagata ctgcggctga
    10261 agaagctcgt ttggatgctg cattagctaa atctactgaa gaattgcagc aaattcgtga
    10321 aaaagcagcg caaagcttag gtgaagcaga agcgcaagta tttgatgccc atttgatggt
    10381 tctttcagat ccagaaatgg taggtcaaat caagcaaaat attaaagaca acagcgtaaa
    10441 tgctgaatca gcgctaaaag aagtaacgga tatgtatatc ggtatgttcg aagcaatgga
    10501 agataatgct tatatgcaag agcgtgctgc agatattcgt gacgttgcta aacgtatttt
    10561 agcgcactta ttaggcgtta ctttaccaaa tccatcaatg atcaacgaag aagtggtcgt
    10621 tgttgcacat gacttgactc caagtgatac agcacaatta gatcgcaatt ttgtaaaagc
    10681 gtttgtaaca gacattggtg gacgtacttc acattctgct atcatggcac gttctttaga
    10741 aattccagca atcgttggga caaaagaaat tactgctaaa gtcaaagaag gcgatatttt
    10801 ggctgtcaac gggatcgaag gcgatgtaat catcgatcct acagatgaac aaaaagctga
    10861 atttgaaaaa gcaggtgcag attacgcagc acaaaaagca gaatgggaaa aattaaaaaa
    10921 tgcagaaact gtcacagctg acggcaagca ttttgaatta gctgcaaaca tcggaacacc
    10981 aaaagatcta gttggtgtcc ataataatgg tggagaagca gttggtctgt accgtactga
    11041 attcctgtat atggattctc cagacttccc aacagaagat gaccaatatg aagcttacaa
    11101 agctgtatta gaaggtatgg aaggaaaacc tgtagttgtt cgtacaatgg acatcggtgg
    11161 agataaagaa ctaccatatc tgcaattacc tcatgaaatg aatccattct taggatatcg
    11221 tgcattgcgt atcagtttat ctgaacaagg cgacgaaatg ttccgtacac aaatgcgtgc
    11281 attgcttcgt gcatctgttc atggtaattt acgtatcatg ttccctatgg tagctacact
    11341 caaagaattc cgcgcagcaa aagcgatctt tgaagaagaa aaacaaaaat tgataagtga
    11401 aggcaaggaa gtatctgata cgatccaagt cggcatcatg atcgagattc cagcagcagc
    11461 agtcttagca gacaagtttg caaaagaagt agatttcttc tctgtaggta cgaacgattt
    11521 gattcaatac acaatggcag cagaccgtat gaacgaacgc gtttcttact tgtaccaacc
    11581 atataaccca tcaattttac gtttgatcaa aaatgtaatt gatgcagcac acgctgaagg
    11641 aaaatgggca ggtatgtgtg gagaaatggc tggagatcaa acagctgttc cattgctagt
    11701 aggtatgggt ctagatgagt tctcaatgag tgcgacatct attcttaaaa cgcgtagttt
    11761 aatgaaacgc ttggacacag cgaaaatggc tgaacttgct gatcgtgcat tgaaagaatg
    11821 cgatacgatg gaagaagtcg ttgaacttgt tcatgaatat gtaaaataaa caacgtatca
    11881 aaagctggtg tggcttgtaa aagagctacg ccagtttttt tgttcttttt taagaaaacg
    11941 tttttgtcag taagaataga caaaaatagg gcttaagttt gagtgacagt ttgtaggcta
    12001 tgttatacta tataagtgaa gggggacgta aggtgaaggt attattatat tttgaaagtg
    12061 aaaagatgct tgcaaaatct ggaatcgggc gtgcgcttga tcatcaaaaa cgtgctctga
    12121 cggaagtcgg tatctcttat acactagatg aaaaagaaga ttatgacatc ctgcacatca
    12181 atacatacgg aatcaatagt cataatatgg tcaataaagc cagaagagaa ggaaaaaaag
    12241 ttgtctatca tgcgcattct acagaggaag atttccgaaa ttcctttatc ggatcgaacc
    12301 agctttctcc tatcgtaaaa aaatatctgg taggattgta ccaaaaagcg gactatctga
    12361 tcacgccaac accttattca aaacagttgt tggaaagcta tggtatccgt gtgccgattc
    12421 aagctatttc caatgggatc gacttagaga aatatcaccc tgatccaatg aaagaacaaa
    12481 agttcagaga atatttcaaa ctgtctccgg atcaaaaagt aatcatttgt gtcggtttgt
    12541 ttttcgaacg taaaggaatc attgatttcg tagaaattgc taagaagatg cccgaataca
    12601 catttatttg gtttggtcat gttcccatgt actcgattcc tcggaacatc cggaaaattg
    12661 taaaagaaga tcatcctgaa aatgtcctgt ttcctggtta cattcgtgga gagatcatag
    12721 aaggtgcgta ttcaggtgca gatctattct ttttcccttc ttatgaagag acagaaggga
    12781 tcgttgtttt agaagcttta gcaagcaaac aaaacgtact tgttcgagat attccagtgt
    12841 ataatggctg gttagaagat agcaagaact gctatatggg gaagacaaac gaagaattta
    12901 tccgactgat ccaacagatt accaatcaag aactaccgag tacgacacaa gccggttatc
    12961 aaacagcaaa agagcgaagt atcaataaaa tcggtgaaga acttaaaaat gtatacgaaa
    13021 gtgtactgaa tgaaaaagtc tctagcaaat taaaaaaagt aaatcaggtc aaggactagt
    13081 ttggaggcga aaaacatgaa aatcggcttt tttaccgata cctactttcc gcaagtcagc
    13141 ggagttgcta catcgatcaa aacattaaaa caagaattag aaagacatgg gcatgaagtc
    13201 tatattttta caacgaccga tcctaatgcg gataagttgg aaaaagatgt gattcgaatg
    13261 ccaagtgtac catttatttc tttcaaggat cgaagagttg ttgttagagg catgtggtat
    13321 gcttatttgg ttgccaaaga actagaacta gatcttattc atacccatac agaattcgga
    13381 gcagggatac ttgggaagat ggtagctaag aagttgaaaa tacctctgat ccatacttac
    13441 catacgatgt atgaagacta ccttcattat atcggtaaag gaaaagtgat caggcaatcc
    13501 catgtaaaat acttgtctag attatttgca aatcatacga caggtgtagt ttgtccaagt
    13561 gaacgtgtta ttgatacatt acgcagctat ggtgtaatag caccgcttcg cgtgattcct
    13621 actggtatcg acattgaaaa attcaaacgt tctgatatca ctcagcagga tatcagcaaa
    13681 ttaagggagt cactaggtct tcaggaaaac catttgatga ttctttcttt aagcaggatt
    13741 tcttatgaga aaaatatcca agccttgatt aatgggtttc ctcaggtcat ctccgcttat
    13801 cctaacgcca gattagttat cgtaggaaaa ggaccttatg tagaagagtt agaggaattg
    13861 atctcagaac tgcagttaga agaatttgtg cagttcacag gagaagtgga caacaatgat
    13921 gtagcacttt attataaagc ggcagattat tttgtcagtg cttctacttc agaaacacaa
    13981 gggcttactt atacagaagc gatggcagcc ggaacacctt gtgtcgtgga aggaaatgct
    14041 tatttgaacc gattgttcga tcatccttca ttagggaaaa cttttgaaac cgacgaagac
    14101 tttgcaccag cacttattga ttatatttcc agtgaagttc ctaaagatcc aaaagtttta
    14161 gaagctaaac tttatgatat ttcatctaca catttcggcg aagctatgat cgaattttat
    14221 caagacatgg ttgtttatca tgaagaagag atgcgaagaa aagaagaaga agactcgatt
    14281 gaacgaatca aaataaaatt aaattcattt aaaagataaa attcaaacat tttttatgaa
    14341 aaaatccttg tttctagcgt atactagcat agaagcaagg atttttgatt tttaggaggt
    14401 cgaaaaaatg aaacttggtt ttatcggaac aggcgtcatg ggaagtgctg tcgcacgcca
    14461 tctactagaa gcaggacacg aagtagctgt ctataatcgg acaaaagcaa aagctgatcc
    14521 tttagtaaca gaaggagcta tctgggcaga tacaccaaaa gcggtagcag aacaaagtaa
    14581 tattctcttt acaatggtag gttaccccaa agatgtagaa gaaatctact atggacagtc
    14641 ggggattttt tctgcagata tctcaggaca tattttagtt gatttgacta caagtacacc
    14701 aagcctggca gaaaaaattg caaaaacagc aaaagaaaaa ggcgcagatg cgttagatgc
    14761 accggtatcc ggtggtgact taggagcaaa gaatggtaca ctgacgatca tggtcggtgg
    14821 agaagaagcc gtttatgatc aagtactgcc tttattcaaa gaatttggta cgacatttac
    14881 attgcatggt tctgctggga aaggtcagca tacaaaaatg gctaatcaga tcatgattgc
    14941 aggtacgatg acaggaatga cggaaatgct tgtctatgca caaaaaaacg gacttgattt
    15001 aaagaaagtg attgagacgc tttcaggtgg aagtgcagct aactggtctt taagcaatta
    15061 ttctcctcgt attttaaaag aagattacac acctggtttt tttgtcaaac attttatcaa
    15121 agatttaaag attgcattag aagaagcaga aaaaatggat cttgtattgc cagcaactac
    15181 ccaagcattg aaactatatg aagagttggc agataaaggt tttgagaatg acggaaccca
    15241 agctttgatc aaactttggt ggcctgaagg aaaaatacca gaaaaaaaat cataaaaaac
    15301 gagttttttt agagaaaatc aatgcaagta ggctatgctt ttgatacaat ataacagaaa
    15361 taagaaaagg aggacctccg atgacacatt cacaattagt cgcaatcata aaacgactgg
    15421 aggctatggt cgagtcagca gataatgaac tgcaagttcg ccgttttgaa aaagaaggcg
    15481 tagagaagtg tacagtaact tatgataaag cgaccgatac atttgaatta actgaaacag
    15541 atactcatca gcaatacgaa tttgataata tcgatatcgt agcgatggaa atctatgact
    15601 taatccaata aattttagga taattgacat ttgaagttga agatgcctaa aagaagccga
    15661 gaagcaacta atttagttgc ttttcagctt cttttttgtt taacagttca atttcaaact
    15721 gttctttttc taattttttg cggtaactga tcaagatttg tacaggatcg atttcttctt
    15781 ttgacaattg acgtttcaaa gataaatgag cttctttctt ttcataatag aagtcaggag
    15841 aaacgtcacc agaggtaatc attgtttctg caagttgacc ataacaaacg aggtagtgct
    15901 ttttctgact ctcatctgct gctaatggca tgctgaaggt gagcgatggt gttcttaaaa
    15961 actgcttttg ttcctggatc cgtctaataa aataagtaac taattcgata tctttttgaa
    16021 gcataccggc tttttcgaaa ttcaactgat agatgaaatg ttgttgtctt tttttcaagt
    16081 aagtcagtag ctttttattt cggccttgaa acagcccttg gagttccttt tttttctgtt
    16141 caaaagtcat ttcctgtagg tcaggaagct ggactcgcaa agctaacaaa ctaatttcag
    16201 acaaccaagg catttgataa gtttcttcca ttatacgcaa aatagacggc atttttttat
    16261 agagacgaaa aggcccgtat gttcgagccg taggagtatc tgttaataca aatcccttat
    16321 tggtaatatg tacataatta taattactga aataattcat ttgtcggtta taaaaaggac
    16381 ggtaatgttg gatcaattga cactcgagca ataaagcatc taattccgtg tctactacga
    16441 cgaaatcaaa atctgttata tgatgaatca tacgtaatac tttttttgaa tgctgtttat
    16501 tctgatgaaa atagctgctt actctatttt tgagccgttt tgctttacca acatagatga
    16561 ccttaccatc tcggtctttc ataagataga cacccggcag catggggaga gttttcattt
    16621 tttcttttaa cataggatct ccttttacgc tttgaataaa aagactataa caaattttga
    16681 aataatggac aagttttagg gaattctata gttctgttc
//
LOCUS       DASJRQ010000059        16663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJRQ010000059 DASJRQ010000000
VERSION     DASJRQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.059"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1392
                     /locus_tag="U4A80_001797"
     CDS             <1..1392
                     /locus_tag="U4A80_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1975421.1"
                     /translation="KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYE
                     GVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPAL
                     PEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSD
                     EVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVD
                     VSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNA
                     EVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNK
                     KDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTL
                     GLLGYKNRKKENE"
     gene            1526..2869
                     /locus_tag="U4A80_001798"
     CDS             1526..2869
                     /locus_tag="U4A80_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1975422.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2981..4384
                     /locus_tag="U4A80_001799"
     CDS             2981..4384
                     /locus_tag="U4A80_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1975423.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4733..5365
                     /locus_tag="U4A80_001800"
     CDS             4733..5365
                     /locus_tag="U4A80_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1975424.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5953..7392
                     /locus_tag="U4A80_001801"
     CDS             5953..7392
                     /locus_tag="U4A80_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1975425.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7687..7905
                     /locus_tag="U4A80_001802"
                     /pseudo
     CDS             7687..7905
                     /locus_tag="U4A80_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8154..8369
                     /locus_tag="U4A80_001803"
     CDS             8154..8369
                     /locus_tag="U4A80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975426.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8586..8842)
                     /locus_tag="U4A80_001804"
                     /pseudo
     CDS             complement(8586..8842)
                     /locus_tag="U4A80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9029..9940)
                     /locus_tag="U4A80_001805"
     CDS             complement(9029..9940)
                     /locus_tag="U4A80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1975427.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9959..10963)
                     /locus_tag="U4A80_001806"
     CDS             complement(9959..10963)
                     /locus_tag="U4A80_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1975428.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10960..13083)
                     /locus_tag="U4A80_001807"
     CDS             complement(10960..13083)
                     /locus_tag="U4A80_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1975429.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13088..15535)
                     /locus_tag="U4A80_001808"
     CDS             complement(13088..15535)
                     /locus_tag="U4A80_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1975430.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15522..15911)
                     /locus_tag="U4A80_001809"
     CDS             complement(15522..15911)
                     /locus_tag="U4A80_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1975431.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15971..16474)
                     /locus_tag="U4A80_001810"
     CDS             complement(15971..16474)
                     /locus_tag="U4A80_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1975432.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16487..16648)
                     /locus_tag="U4A80_001811"
     CDS             complement(16487..16648)
                     /locus_tag="U4A80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975433.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         5384 a         3101 c         3073 g         5105 t
ORIGIN      
        1 aaagtagaag ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta
       61 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
      121 ggtaattacg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
      181 gttccagtag aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa
      241 ggcgaaaaag tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca
      301 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      361 acaccaggca attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      421 gtgaaagttc cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa
      481 ggacaaaaag tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac
      541 aaagaagatt taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca
      601 gctggaaata aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt
      661 gaagtagacg ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga
      721 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      781 gaagatctac caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct
      841 ggaaacaaaa aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa
      901 gttactattt ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca
      961 gagggtgacc aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta
     1021 ccagacggaa gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt
     1081 cctaaattag aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact
     1141 agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt
     1201 aaaggtagta agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa
     1261 agccagtcct caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt
     1321 agcggtggtt taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa
     1381 gagaatgaat agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct
     1441 tcctcttttt gcttttttat gatgggaaat aagtgttagt taaagatgaa tatacttttt
     1501 atagaaagga aaagaatgta attaagtgga aaaaatagta attattggtt caaaccattc
     1561 agggctagcc gcagctcgat ttctaaactt aagcgattca aattatgaag ttattctaat
     1621 cgataaaaaa tcagaaatta gttacttgga atgtgggaca tcgttattta taaaaggaga
     1681 agtaagtaat ttagaagatc ttttttatca aaatagaatg acactaaatt ctgagataaa
     1741 aaagttattt ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg
     1801 tgtaaaaaaa gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg
     1861 ttcaaagcaa attatgctag atataccggg gaataattta gctaatattt tttcgataaa
     1921 gaattttcca aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt
     1981 aggaattatt ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg
     2041 gaaagaagta tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag
     2101 tttttcagat aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga
     2161 aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac
     2221 ccaatactct atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg
     2281 ggaaaaggaa ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac
     2341 aaatataaaa gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa
     2401 tagaagtagt gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca
     2461 acatattaac aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat
     2521 atttaattac aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaagtga
     2581 gtctatagat tatatagaaa tagaagattt cattagacct gcttttttga gaaaaaatag
     2641 tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag agaatagtag gtgtacaact
     2701 ttgtagtcaa gaagatttat ctggactatt atcaatgttt tctcttgcta tagaagaaca
     2761 aattacatta gacaaattga aaatgttaga ttatttattt catcctagtt ttagccaacc
     2821 ctataatttt attttaaagg cagtaacagg attaaacatt gaaacataat cgttactaaa
     2881 tgaaatcaag aaatatactg tattactcaa ttcattgaaa atatttatca taatttttaa
     2941 tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact
     3001 tgcatcagca gttatattaa tgtcccaact ccttatagca acacctttcc atgtgttggc
     3061 aagcgaaaat tcaacataca aaattgcaaa agaaagaacc gagacgactg aaatgagtaa
     3121 ttccgatgta acagcagaga gtagtgaact tttgacaact tcatcaagtg aaagtagtac
     3181 gacttcaaat gccgagtctt caacaacttc atcaagtgaa agtagtacga cttcaaatgc
     3241 cgagtcttca acaacttcgt caagtgaaag tagtacgatt tcaagtaccg aatcttcaac
     3301 aacttcaagt agtacaagtg aaataacttc ttcgaaagtg acaaaaccaa accatataga
     3361 atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca aaaacttttt cgacaccaca
     3421 gcaaatagaa tcaacagaat ctttccaatc acaaccaata ctatctggta atgaagaaat
     3481 tcattttgac aaaaatgaat ctgtagaaaa ttttattatg aaaattggtg agtctgcgag
     3541 aaaaattggt cagaaatatg atttatatgc ttctgttatg attgcacaag cgatcttaga
     3601 atctgccagt ggtcaaagcc agttagctca ggcaccgaat tacaatttat ttgggataaa
     3661 aggaacttat aatggaaact ttgtcattat ggtaacaaac gaagatttgg gtaatggaac
     3721 gctttatacg acccaatcaa aatttagagt gtatgaaaac tatgaggaaa gctttgaaga
     3781 ttatgccaaa ttattaacta agggaattag tggaaataaa gatttttatg caggagcatt
     3841 aaaagctaat agtaaaacat atcgagaagc aactaaattc cttacaggac ggtatgcaac
     3901 agatacacaa tactatttga agctaaatga attaattaaa acctacgatt taaccaacta
     3961 tgataaagaa gttaaaggtc cacaaatgag taagactgga taccaagtgc ctattcataa
     4021 ttattcaata accagtttgt ttggaaatcg tggaaatgaa tttcaccgag gacttgatat
     4081 ggcagctaca cagggtgagc cgattcatgc agttaaagct ggtaaggttg ttaaagcaga
     4141 gtttcactac tcttggggaa attacgtggt gatacaacac gaagatggat caaccgcgtt
     4201 atatgctcac cagcaacaat acctagtaaa aactggagat aatgttttgc aaggacaaat
     4261 aattggctat gtaggaagta ctgggaatag tactggccca caccttcatt tggaaatttg
     4321 caaagatagt agtctttctc agagcatgtt agttgatccg aaaaccttat tattcggaga
     4381 ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg gttggattct tagacctttg
     4441 atcaggatat tgagactgga attgatatct cgattacgat gggtttgaca aataaaacaa
     4501 gtccattatc gaatatcgag tgtattattg ccgtctttat gctcatattt tgagcaggtt
     4561 tcactagatg aaaacaattt atctatcttg aggatttcgt gtacgtatcg ttcagtcttg
     4621 tattgtaaat tagtcataaa atcagactaa gaaaaattgg aaatattttt ttctgattta
     4681 ttaaaagtta attgaaatta tatggtactt ctttcttggg agggtaggtg cagtgaagga
     4741 aagatatggt caccttactg ttgttgagga gattactcat aatggatata aaaaatatcg
     4801 ttgcctatgt gactgtggga atgaaacgat tgtatatcga tcaaacttac tatcaggtag
     4861 aaccaaaagt tgtggatgtg gaaattataa aaataggcat aaatataaaa atttgacaaa
     4921 taaaactttt ggtaagttag ttgcaatcaa gccaactaaa ctgcgaagtg aaggaactgt
     4981 tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta gtatcggcaa agcgacttct
     5041 aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa aagcatcaca ttgaaaataa
     5101 aaaattcgga ttacttactg caaagtatcc tttggacaat aactgttcat ggaaaacaaa
     5161 atgggtttgt cattgtgagt gtggcagtat gtgtgagata agttatagta acttattttt
     5221 tggacatact caaagttgtg gatgtttaaa aaggcaagaa acaaggacat gtgttgaagg
     5281 gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat aatacaagtg gtgttaaggg
     5341 agtttatcgt tctaaagaga aatagattgc gtatataact ttaaaaaaaa agaggtacta
     5401 tttaggatcg tatgattcta ttaagagtgc agctcaagct agaaaagatg ctgaaaataa
     5461 attgtttcaa cctttgattg acaaatatcg aggtaatcat taattattgt ggataataat
     5521 taacagtgat atataaacat ctataatata ttattcatac tattgatatt tagtataatc
     5581 ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa attggaaaac aaataggtgc
     5641 aacaccataa atatgaaaaa attgggaaaa agtagaatgg aaatttgctt acgtctcacg
     5701 agatagctca cactattaat ctgaagaaca atgaagtcga cttttagaaa ttgctagaag
     5761 aaagtaaagc gaattatcac tagttactat agagtaagtt caaataaagc atctccaact
     5821 tacatggtta catgaggata tcaaatcgaa attactcaag cttcctatgg ctagagtcgc
     5881 gagaagttgt tgggcagtag acataggtta tattgttctt agctacacga tcattagaaa
     5941 ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag tccaaaaaaa tcgctggtgg
     6001 attttgattg ctatatctat gtttaccttc atgtccacat tggatggcag tattgtcaat
     6061 attgcactac ctaccatctc agatgatatg ggcataccaa tgaatcaagc ggaatgggta
     6121 gtgtccgttt atctgatgat tgtctgttct tgtttgttgc tgtttggtaa aattggcgat
     6181 agccgtggga aaatcaaagt atttcggatt ggtacgctga tctttgtctt gggatcgctg
     6241 ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg cacgggtggt acaagccatt
     6301 ggtgctagta tgactatggc tacaaatgcc ggaatcatca cggaagtctt tcccatacat
     6361 gaacgtggtc gggcattggg agcaataggt gcgtttgttt cattaggctc gattgctgga
     6421 ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg cgtacatttt ttggatcaat
     6481 gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat tcttaccaaa agatatcacc
     6541 atgagcggcc atacaatcga tggagggggc tttgctgcca ttgcggctac gattatcagc
     6601 ttttttggag caatcttttt ggggcaagaa agtggctttt tccatccagt cccccttgta
     6661 ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa aagttgaaaa gaaagcagcc
     6721 tctccattga tttctttcgg tatttttaag aacaagattt ttaccatgag cttgattaca
     6781 gccttgttga ttttttcttc caacttcttt gtcaatgtag tgattccttt ttatctgcaa
     6841 aatgctcgcg ggttgagtcc tagctatgct gggctgttga tgatggtctt tcctttgatg
     6901 atggtcgtcg gggcgccttt aagcggctac ctgaccgacc gggttggccc ggagattttg
     6961 gtattagtag gcttgggctt attgtcggta acccaggtca tgtatatgtt tttgtcagtg
     7021 gactctcctt tgtggctata cattgtcgct actgccatca tgggtgccgg caatgccttg
     7081 ttccagtccc ccaataatac gatggttatg agctcggtca ctatcgaaaa cttgggactg
     7141 gcaggctcga tgaattcctt tgcccgaaat ttggggatgg tcagcgggat ttcgctttct
     7201 acgacggtct tatatcaagc gatgtctgct aagatgggcc agcaagtaac taactatatc
     7261 gcaaatcggc cggatgtctt tttatatgga atgaagatta cctttttagg cagctttgtt
     7321 ttgtgttttg ttgccttttt cctgactatc tggcgtatca aaaaacaagg aggtattcag
     7381 aaaaatgtct gaaaaaacca ctgttgaaga atttttggag gcctgcgtat gggtgagtgg
     7441 gatttccatg gatgatggaa gatgaagtga acttctaaaa gccgaaaaac tcaagtatta
     7501 tcttcaaaat actagtgctc aatgagatgc ttgatttgat cacaagaaga ctgcgaaagc
     7561 cgctggcaac cgcaagctag gtatcccacc gatggactac gttttagagg ctactggtta
     7621 tgaatacgga gccaacgctc tcatctgcat aagaatacat cacccagatt atgtaaagat
     7681 tttcgatact tctgtcaaaa aatcattaag atagataaat gatttccgtc tagtcaaatt
     7741 ggttcagaat gtagacacac agatggtaag aagtcactca caattcgaga atggacttgt
     7801 cctatttgcc gtatacatca taatcgaaac gtcaacgata gtatcaatat tttgaccaag
     7861 ggctatgaat acaagcattg ccttagaaaa catcaagaac cttaggaaca acggggatag
     7921 cttggttaat aagagatacc gctgtttagg cttgcaagta tactctttta tcaagaattc
     7981 cgtggcttta gtcatgagag gcttaatact atgaaaattt tctttagcat ttttgattta
     8041 ggttcctttt ctaattataa taagatgacc actacagtaa cctactgaaa tatataaaca
     8101 gccactgtag taccattttc aaagtagtct atgagaggag aatcttcatt cttatgaacg
     8161 ctggaattgc ggatatgaat ttaataaaaa aaacccttaa tgattttact tccaattcaa
     8221 tatcaaaagg tactggtatc aatttaagca caataaaaaa gctgaaaagt ggtgaaagat
     8281 cagtggaaaa gctgaatctt ttagatgcta taaaaataac ggaatttgct atgaaaaatg
     8341 gaaaagctga gattgaaata tggagataat tatcgtctag tgaattactt gccacttagc
     8401 tgaatttaac gatccttggt gcttgaaggg ggagattctt gggaatagag catacttgca
     8461 agcctatgtc tttactaaca acgatatctc ttattaacca agctatcccc gtagttctaa
     8521 ggcttttact tttatctaag caataatcgt attcaaagga aatttttgac ccttagaaag
     8581 tctcatttta tcagacgtga gcttaggcat tgctgaattt tgttaattcg gttttaggtt
     8641 gtgctttgag cataatatga aagtattctt tatcacgatt tcattcatct aaggtaatgt
     8701 gatgtgattc tgaaattctt tcaaaggcag tttttgcata agctgacatt acatcatcaa
     8761 tgacatgttt acaatgtttc actactaaaa taagggggta accaagaaga aaaactgaat
     8821 gattatttgt atctaattcc atggtatatc aaccatttcc tatatagact gattatgaca
     8881 tggattaaaa taaaaaggca ctatgtctta attgtcggtt ttcgagttac cgaatggcat
     8941 tcatcaccca cctatagagg atgggcgact tctgcctgaa ataagttaca ctacttataa
     9001 cttctaataa aattagattg agtcattatt atttaacatc aacgattttc cagtttccat
     9061 ttttttgtaa tactaatttg tactgaaagt agctagtggt ttttgataca ttatcaaggt
     9121 attttatctt tgtactcact agaaaatttt ttccgttttg tagataaact ggattctcta
     9181 actccgaaaa ctttaagtca atattgattg gtcgcatcgc accattttca acataatact
     9241 ccaattcttt ttcagaagct gatggataaa gtttaaaaaa tgtttctaaa aaatcggtga
     9301 tttcttccgt tgttttggca tcaatttctg aatcgttttc tacttgtttt gcttcatatt
     9361 ttgccttgtc tggctttcca atcattgttg gcaaactagt tactaaatga ttcctgtttt
     9421 catcttcaaa gattgtcact cgatatgcag aagaaattga ttgcgtcttt tttccctctg
     9481 taatatgttg ctccactaaa aatgaaacga caaattcttc cgagtgtttt tcaacatcta
     9541 gaatttttac agattgaact tcggaactcg tgggaatatc tgctcgaacc atatcttgga
     9601 ttaaagcgag accgttttct gtaaggtact gttcgagatt actcattctc ttttcaattg
     9661 cttctttgtt atttttccat gaaaaatatt ctttcgcaaa atcctctgta aaactttcaa
     9721 cgccagaagt attcactacc ttctctttta taaccacttt ttcatgagtt gtatgttgat
     9781 caatggcggt gaaattttta taaacgccaa aagcaagact acaagacaat aaaagccaaa
     9841 gggcaagcac catttttcga tgcttaccca cggatacaac tcgttctttt ttcttttttg
     9901 ggctcttttc cttttgattc cgttcaattt ttatcttcat tctttttcct cttttctctt
     9961 atttgtatcg gccagctccg ataagatgct gccgccaata gttttccgtt agattcgtcc
    10021 accccactgg atctcctgca tgaaacatgc gattctcccc tgcgtaaatg cccacatggg
    10081 tcacataagt accggcatca taggttgaat gaaagaaaac taaatcacct ggtttggcat
    10141 cttttagatc aatatgagtc atggcatcat attgatcctg agccacccga ggaagtttca
    10201 ggtcaacttt ggcataacac cattgggtca agccactaca atcaaacccc gtttctggac
    10261 ttgagccacc aaacacatag gccgttcctt catacttaaa tgcctcatca aaaatttctt
    10321 ggactatttt gtcatcaaac tgagttgtag ttaggtattg cttcactaat aaacagtaaa
    10381 acatattgcc ataattatac cgccacccac cattttcctt aatcgcgatt gggttggggt
    10441 aatcgacttt ttgaccacct gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat
    10501 gcttcttccc gtttttcccg acatgatcta gatacccgcc accatagtta taggcctgaa
    10561 taatactatc ttgatcacac cctagttctt tggaacgctt aaccaaagac gcaaaataga
    10621 gacagccttg tttgatggat gcctcggtac ttaatgaatt aggtggtaat ccggctgact
    10681 cagaactttg cataacgtct tccattgtgc caccagattc cacttgaata atcgccaaaa
    10741 gaattggaat atactctgtt atatccgatt ctttggcata cttttccacc attgcacggt
    10801 gtttcaatac ttcttctgag acactagttc cgccaggaga agtgccagaa ctaccatttc
    10861 cctcactgtc ttcaccaaac aacaaaccca cacacatcaa aatacccata aaagagatgg
    10921 tcagaaaagt taatgaaata acacccactt tcttcaagtt cattttttct cacctgacct
    10981 tgattttgga cgatggttca ttcttttttg tactttttga ttggtctgct ttggcagagt
    11041 ggacctctgt ttcacttgtt ggaaagaatt tttcttactt gctgattggc ttttcccttt
    11101 tgaaatgatt tcttgactag ttagttttcg tttgatttcg ggattcagat ttgctctcac
    11161 aatccgcttt ggtgcttctt ttttcagcat catatctttc tgaggttgag ctgctttatc
    11221 tattggaata ttcgcccttc tttttccctg acgatttgtt gcttcactca gtatcttgcg
    11281 tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt tctcgcaaat ccactcgatc
    11341 cgctttttct tggaacagtc cgcgcttgaa ttcttctggt gcttttttcg acttttcgat
    11401 tcctttatgg agattgtatt tcaaattcgt tggcgtattc cttacctgat cctttacttg
    11461 tttagtctta tctttcacca aagcttgggt atccaaaact ttgccgattt ttctcccagc
    11521 caaattcatt cgggattgtt tcttattatt tgaaactgga ttctccttag ttaagtcaga
    11581 tagtatttcg tgatcgttcg gtaatcgtga attctttcgt agagaagaac cggatggctt
    11641 aaatttggat tttgattgtt cctttgccaa cttatttccg actgctgcac caccaccaaa
    11701 agccaaagct cgtcccacat ttcgattgag ttgccgcaat tttcgattca ttaacatttg
    11761 cggttttcgc atgacgcgtc ttcccattga ctggctatca ttactctgga ggttgaacat
    11821 actcataatg tcgcccaatt tgaaatagat accggcaaag gtcacaattt gcagaaaagc
    11881 aaccatgaaa aatggatagg ttgccgaaat attgaaaaac atcgtggaga tgctaaatgc
    11941 agtcgtaatc accaaagtaa cacccgctcg catcataatc gtattgaata aacgaataat
    12001 cgccttcttt cctaaacttt cataagtcgg caacatggac aataagaaac taattggcag
    12061 aaacatcgca aagataatga agagaatttg cgagaacaac ataatccctg ttagaagaaa
    12121 aacaaagaag gaaatgccaa tattgaacaa taccaaaaag accacggtcc ctaaacggtt
    12181 catggtcttg gtaatactga gattcgcatt gtcattgtct tcaatctctg ccttgaccgc
    12241 ttcttcccga tctttgcctt tgttttcatc tggactcaca gatagaatct tgttgacccg
    12301 gtcttcacca atttcttcta tgttggaatc atcaaattgc agtagtaacc aaggttgttg
    12361 gacttgaatc gaaaacaaac tatcccgaat caaatccaca ctatcttttc cttggctttc
    12421 tgaattgggt acaacgatct cggttccaag ggtaagggcc gcttcactaa catctgagct
    12481 aaagtcattg atttttgtga tataggtagg cgcataagca atgaacgaac ccgatagtag
    12541 aaaaatcatc acaaaattaa tcacggctcg aactgctttc gtggtttctc gttttaataa
    12601 tccggtatac gccacataca cccccagagc taaaatcatc aataatagaa acccaaaata
    12661 aaatccggag gtctggagtc cattttcagt aataccagcc aatgtttgga tattctttcc
    12721 aatactttca gccgtatccg aaatgaaatc taatttgtaa gcctcttgaa ccacataacc
    12781 cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa ttagtaatgg cataaagacc
    12841 atacatcacg cttttcccta agccatcacc ccaattccaa ggcaaccaat cccaagagct
    12901 atccacaaag aaatctagtt gatagttgtt cagtgaatat ttggaatata gatttcctgc
    12961 ttcaactgta tcatcaacta gccctgtcgc ctcggctacc gtaccaacta aactcaatag
    13021 aactatcaga gtgagtatgc taataaaaac gattcccaca atccgaaaaa gtttcttttt
    13081 catcccctca ctccattctc tcttgaacag gtggacgggt atcaaaggca tggaagagtt
    13141 cttcaaagac tggatgtacc tgaaccacgc ctacccgacc atacaaatcc tgcatcaagc
    13201 attggccatt ttccaaatct cgtaggcgtt tttgattgct ttcatcttct cgatccaagc
    13261 caaagaattc caaggttttc ttgatttcat gaatatcggt actgcgaaac gcaaacttca
    13321 acccgatatt attcttcaag cgttcatcca gtaaatcatc actattttgc gtgacaaagt
    13381 aaaccccggc attcatagcc cgcccagcac gaacgagctt attcgataat gttttccctt
    13441 gcgctacttg aagaaaactc cacgcctcat ctaagtctac aattttgaag atttctcgat
    13501 tactatggat aaagtccaag gcaaaagtgg aaatgacgat tagcatggaa acactaagca
    13561 attccatggt ggtatattct tcaaaagtgg tttctgcatc tggtaaaacc aaatccgcta
    13621 cttgaatgat atttaactgc ctatctaaac taatcgactg ggtaatcgaa ccatctgaga
    13681 aaagcaaatg ggcaaaatca taatcggtaa aggactcgat gtgatccgcg atattttccg
    13741 caactggtga gccttctttt cgaagctcat taattacttg taataatccc cgttgattgc
    13801 tttgggtaac cgtccgaatg gccttacgca acactggaaa cttctcccca tctcgactag
    13861 aaattcctgt taaaaaggtc agaatatcaa ttgctagact ttcagaatcc ttctttttct
    13921 ccataatcac ataaggatca agtaatcctt gattttttgg cttactagtt aagttgagaa
    13981 tattgatttc atccgcaatt tctggcaagg tttctttcca gccaccccgt tccccttttg
    14041 gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta ataaactaag agattgttac
    14101 taaaggactt tccgccaccg agtgaaccga ggaatgccgc tgctagcgca ttggtaactg
    14161 aatccttgac cccttgtgag gccaatgccg gtttcaggta aacattgcgc cccgtatcta
    14221 cgttataacc aaagtaaatg ccttccaact cccctaacat ctgagtggcg ccaaaaccaa
    14281 gtccggcgag aaaatcagac gtaacatact ggatataatc attcatgtat cgcttactag
    14341 aaggtaagaa ttcttcatgt agccccatca tatcgccaaa aggccggact agttttacat
    14401 tggtatcatc ataaaaatct agcacttcat cacaccggcg ctttagttca tctactgatt
    14461 cggcacttac tcgaaccaca tagctcagtt tgtacatgga ttctttggac tgatccaagg
    14521 tagtttccaa ttcatctacg gaatccaatg catctaagac attggaattt gtttcattgt
    14581 cagattgata ggcatggttg tccaagtctt ttaattcctt tttcttattg cgaacggtag
    14641 caagtgcctt tttgttcgtc acaatctcca cattcatact agtgtcaatt gggaatgtaa
    14701 attgttgttg ctggtaatag aaaagttctg aggagggaaa ctccatttcc cccacaattg
    14761 tattaattgt taagtacgct acatatgatt catgattctc atgttccatg cataagtacc
    14821 ggggcttttc ttcaatcaaa caacgactgg gacgtaataa gtcgtaacgt ttcaccaaag
    14881 tttccgactt cagctttttc tttggtagct gaaattcata ttcttcaaat ggtgtaccct
    14941 tttcgccata gatatgttca atcaaataca tcagatcact cggtgtgact cgtctgactt
    15001 taaatcttcg agccaattta ctttccatta gtttttcaag cttggtatat cggcgaattt
    15061 cttcattgga taaggaaaca aaatcgccca tcaaatgatg attcactcca tagacaaatt
    15121 cttgaaagcc ggagaagaaa gatttcttga gattctttag attgacctct tcatctgtcg
    15181 caatcagttt gaagccaata aaaaagcgat aatcaatctg actatcgcca atcatggata
    15241 ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac ttctgttaat cgcccggtaa
    15301 tttccttttt tgatcgttct tgcgtggcac gaatactact ttctgtggca atttgtaagg
    15361 catgaatctt gccttcacga ttttgcgcaa tcaattgacg aaagttatcg tggacttgat
    15421 atttctgttc aggtgacaaa aaggagtaat tgtaaggtat taactcatag taggcaaaac
    15481 attctccctc ttgattaaag acaagattat tttcaatata cttaatcggg tacatagttc
    15541 acactcctta ccagagtaat gggttcgtcc catcgtttct tctgttcttt gactttcttt
    15601 cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa aagatcgtaa aaatcccaat
    15661 ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag taactgccac cggtatacct
    15721 aagtatttta aaaaggcccc ctctatcata gatagtggag gcacatgggc aaatacgatg
    15781 acaaacagca aggataaaac aaaccaagcc atctggttaa aagtcacagg aaaaggcagt
    15841 tgaaagtcat taattgcata aatgaccttt tcaactgccc aaatactggt gtagcttttt
    15901 attttcttca tcaaatcatt cctttaaaaa gaaggatagt ttagaaatca actacccttc
    15961 aataattttg ttaatatgga tactcaaaaa cacctcgact cgttaccaaa taattgcctc
    16021 caatctccaa gtctctacca cacgcatcaa aatcaatata cgtcttcaag tgttctggta
    16081 cttctccaaa atcaccagct tctacaatca agaaatatgc tacatcactc atatcatcgc
    16141 aatctggata atagataata tcatccacat gctctaccat ctcctcaaaa gaattgaaga
    16201 aagcatgttg aatctcagaa gcaacttcac caactggcgt tccttcaagt tcctccgcta
    16261 agccacacaa ccgattaatt tcctcaattg gcgtatactc atcaatctca aagggaagtt
    16321 cataatcatg aattgcatat tcctcgtatt cgtcatttaa cccaattcgt tctttcactt
    16381 catcaaaatc gacaggtggg gtaaaccacg cccccaccaa ttcaccttcg ttatatttcc
    16441 cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct tcccccttac gcaccgacaa
    16501 ttcggttaaa caaactgagt aagacatcct tcaccccaga agcattaaag accaatccga
    16561 ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga aaactcccgc ttaaatccta
    16621 aaaacaaacc aatgaccaca attgccagta agactaagga ttg
//
LOCUS       DASJRQ010000060        16220 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJRQ010000060 DASJRQ010000000
VERSION     DASJRQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.060"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="U4A80_001812"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="U4A80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1975434.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(632..1618)
                     /locus_tag="U4A80_001813"
     CDS             complement(632..1618)
                     /locus_tag="U4A80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1975435.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1641..2207)
                     /locus_tag="U4A80_001814"
     CDS             complement(1641..2207)
                     /locus_tag="U4A80_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1975436.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2221..2637)
                     /locus_tag="U4A80_001815"
     CDS             complement(2221..2637)
                     /locus_tag="U4A80_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1975437.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3094..5028)
                     /locus_tag="U4A80_001816"
     CDS             complement(3094..5028)
                     /locus_tag="U4A80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1975438.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5309..6265
                     /locus_tag="U4A80_001817"
     CDS             5309..6265
                     /locus_tag="U4A80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1975439.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6355..7458
                     /locus_tag="U4A80_001818"
     CDS             6355..7458
                     /locus_tag="U4A80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1975440.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7471..8424
                     /gene="menA"
                     /locus_tag="U4A80_001819"
     CDS             7471..8424
                     /gene="menA"
                     /locus_tag="U4A80_001819"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1975441.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9238..10584)
                     /gene="gor"
                     /locus_tag="U4A80_001820"
     CDS             complement(9238..10584)
                     /gene="gor"
                     /locus_tag="U4A80_001820"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1975442.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10718..11971)
                     /locus_tag="U4A80_001821"
     CDS             complement(10718..11971)
                     /locus_tag="U4A80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1975443.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11977..12900)
                     /locus_tag="U4A80_001822"
     CDS             complement(11977..12900)
                     /locus_tag="U4A80_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1975444.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13121..15613)
                     /locus_tag="U4A80_001823"
     CDS             complement(13121..15613)
                     /locus_tag="U4A80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1975445.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15620..16087)
                     /locus_tag="U4A80_001824"
     CDS             complement(15620..16087)
                     /locus_tag="U4A80_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1975446.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4765 a         3394 c         2887 g         5174 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      721 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      781 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      841 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      901 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      961 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
     1021 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
     1081 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1141 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1201 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1261 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1321 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1381 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1441 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1501 tgattgcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1561 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1621 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1681 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1741 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1801 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1861 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1921 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1981 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     2041 cgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     2101 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2161 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2221 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2281 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2341 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2401 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2461 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2521 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2581 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2641 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2701 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2761 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2821 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2881 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2941 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     3001 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     3061 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3121 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3181 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3241 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3301 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3361 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3421 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3481 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3541 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3601 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3661 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3721 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3781 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3841 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3901 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3961 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     4021 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     4081 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4141 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4201 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4261 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4321 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4381 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4441 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4501 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4561 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4621 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4681 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4741 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4801 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4861 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4921 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4981 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     5041 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     5101 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5161 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5221 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5281 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5341 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5401 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5461 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5521 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5581 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5641 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5701 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5761 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5821 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5881 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5941 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     6001 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     6061 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6121 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6181 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6241 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6301 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6361 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6421 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6481 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6541 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6601 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6661 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6721 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6781 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6841 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6901 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6961 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     7021 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     7081 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7141 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7201 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7261 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7321 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7381 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7441 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7501 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7561 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7621 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7681 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7741 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7801 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7861 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7921 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7981 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     8041 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     8101 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8161 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8221 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8281 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8341 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8401 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8461 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8521 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8581 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8641 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8701 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8761 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8821 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8881 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8941 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     9001 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     9061 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9121 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9181 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9241 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9301 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9361 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9421 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9481 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9541 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9601 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9661 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9721 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9781 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9841 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9901 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9961 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
    10021 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
    10081 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10141 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10201 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10261 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10321 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10381 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10441 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10501 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10561 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10621 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10681 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10741 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10801 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10861 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10921 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10981 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    11041 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    11101 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11161 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11221 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11281 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11341 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11401 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11461 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11521 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11581 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11641 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11701 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11761 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11821 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11881 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11941 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    12001 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    12061 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12121 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12181 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12241 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12301 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12361 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12421 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12481 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12541 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12601 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12661 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12721 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12781 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12841 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12901 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12961 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    13021 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    13081 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13141 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13201 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13261 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13321 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13381 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13441 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13501 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13561 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13621 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13681 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13741 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13801 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13861 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13921 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13981 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    14041 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    14101 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14161 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14221 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14281 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14341 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14401 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14461 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14521 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14581 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14641 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14701 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14761 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14821 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14881 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14941 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    15001 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    15061 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15121 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15181 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15241 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15301 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15361 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15421 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15481 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15541 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15601 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15661 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15721 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15781 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15841 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15901 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15961 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    16021 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    16081 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16141 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16201 ggaatatact attccgtatc
//
LOCUS       DASJRQ010000061        16100 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJRQ010000061 DASJRQ010000000
VERSION     DASJRQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.061"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4A80_001825"
     CDS             226..894
                     /locus_tag="U4A80_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1975447.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="U4A80_001826"
     CDS             complement(1094..1324)
                     /locus_tag="U4A80_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975448.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="U4A80_001827"
     CDS             complement(1451..2833)
                     /locus_tag="U4A80_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1975449.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="U4A80_001828"
     CDS             complement(2989..4176)
                     /locus_tag="U4A80_001828"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1975450.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="U4A80_001829"
     CDS             complement(4183..5214)
                     /locus_tag="U4A80_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1975451.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="U4A80_001830"
     CDS             complement(5260..6684)
                     /locus_tag="U4A80_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1975452.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="U4A80_001831"
     CDS             6859..7764
                     /locus_tag="U4A80_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1975453.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="U4A80_001832"
     CDS             7855..8532
                     /locus_tag="U4A80_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1975454.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="U4A80_001833"
     CDS             8529..8699
                     /locus_tag="U4A80_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975455.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="U4A80_001834"
     CDS             8813..9709
                     /locus_tag="U4A80_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1975456.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="U4A80_001835"
     CDS             complement(9764..11134)
                     /locus_tag="U4A80_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1975457.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="U4A80_001836"
     CDS             11372..12118
                     /locus_tag="U4A80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1975458.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="U4A80_001837"
     CDS             12115..12954
                     /locus_tag="U4A80_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1975459.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="U4A80_001838"
     CDS             12955..13905
                     /locus_tag="U4A80_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1975460.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="U4A80_001839"
     CDS             14046..14798
                     /locus_tag="U4A80_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975461.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="U4A80_001840"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="U4A80_001840"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1975462.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5222 a         3003 c         3235 g         4640 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt
//
LOCUS       DASJRQ010000062        15973 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJRQ010000062 DASJRQ010000000
VERSION     DASJRQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.062"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..756)
                     /locus_tag="U4A80_001841"
     CDS             complement(<1..756)
                     /locus_tag="U4A80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1975463.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
     gene            complement(1139..2407)
                     /locus_tag="U4A80_001842"
     CDS             complement(1139..2407)
                     /locus_tag="U4A80_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1975464.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(2638..3375)
                     /locus_tag="U4A80_001843"
     CDS             complement(2638..3375)
                     /locus_tag="U4A80_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1975465.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(3442..4011)
                     /locus_tag="U4A80_001844"
     CDS             complement(3442..4011)
                     /locus_tag="U4A80_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1975466.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            4139..5011
                     /locus_tag="U4A80_001845"
     CDS             4139..5011
                     /locus_tag="U4A80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1975467.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5353..6225
                     /locus_tag="U4A80_001846"
     CDS             5353..6225
                     /locus_tag="U4A80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1975468.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            6222..8180
                     /locus_tag="U4A80_001847"
     CDS             6222..8180
                     /locus_tag="U4A80_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1975469.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(8512..9363)
                     /locus_tag="U4A80_001848"
     CDS             complement(8512..9363)
                     /locus_tag="U4A80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1975470.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            9486..9953
                     /locus_tag="U4A80_001849"
     CDS             9486..9953
                     /locus_tag="U4A80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1975471.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(10216..10698)
                     /locus_tag="U4A80_001850"
     CDS             complement(10216..10698)
                     /locus_tag="U4A80_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1975472.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(10798..11661)
                     /locus_tag="U4A80_001851"
     CDS             complement(10798..11661)
                     /locus_tag="U4A80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1975473.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            12259..13134
                     /locus_tag="U4A80_001852"
     CDS             12259..13134
                     /locus_tag="U4A80_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975474.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(13279..13431)
                     /locus_tag="U4A80_001853"
                     /pseudo
     CDS             complement(13279..13431)
                     /locus_tag="U4A80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(13699..15030)
                     /locus_tag="U4A80_001854"
     CDS             complement(13699..15030)
                     /locus_tag="U4A80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975475.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(15179..15892)
                     /locus_tag="U4A80_001855"
     CDS             complement(15179..15892)
                     /locus_tag="U4A80_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1975476.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT         4842 a         3173 c         2817 g         5141 t
ORIGIN      
        1 tttttgttcg agtaaatctt ggagaagttc ggcgtaatag atcgtttttt gacagtggat
       61 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
      121 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
      181 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatgtcaa
      241 tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc tctggaaaag tcaattgaag
      301 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
      361 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
      421 tttaagatga gtctgtgcga gccggtgggc atccgattta tctgttttcc aactgcgtaa
      481 ggtgctttct tccatctgtt ttttcgcctc taacgggttt aacaaacagt aagaaaagtg
      541 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
      601 caatggacgc tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
      661 gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
      721 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
      781 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
      841 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcgt
      901 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
      961 tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt tttgagtaaa
     1021 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
     1081 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
     1141 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
     1201 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
     1261 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
     1321 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
     1381 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
     1441 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
     1501 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
     1561 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
     1621 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
     1681 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
     1741 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
     1801 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
     1861 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
     1921 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
     1981 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
     2041 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
     2101 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
     2161 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
     2221 aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc
     2281 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
     2341 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
     2401 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
     2461 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc
     2521 tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
     2581 tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta
     2641 tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc
     2701 tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc
     2761 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
     2821 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
     2881 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
     2941 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
     3001 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
     3061 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
     3121 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
     3181 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
     3241 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
     3301 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
     3361 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
     3421 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
     3481 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
     3541 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
     3601 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
     3661 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
     3721 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
     3781 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
     3841 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
     3901 cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc
     3961 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
     4021 tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact
     4081 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
     4141 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
     4201 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
     4261 agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg
     4321 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
     4381 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
     4441 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
     4501 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
     4561 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
     4621 ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat
     4681 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
     4741 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
     4801 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
     4861 tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca
     4921 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
     4981 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
     5041 tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt
     5101 tgttccttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
     5161 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
     5221 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
     5281 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
     5341 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
     5401 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
     5461 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
     5521 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
     5581 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
     5641 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
     5701 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
     5761 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
     5821 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
     5881 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
     5941 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
     6001 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
     6061 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
     6121 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
     6181 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
     6241 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
     6301 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
     6361 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
     6421 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
     6481 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
     6541 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
     6601 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
     6661 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
     6721 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
     6781 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
     6841 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
     6901 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
     6961 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
     7021 aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg
     7081 gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc
     7141 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
     7201 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
     7261 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
     7321 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
     7381 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
     7441 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
     7501 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
     7561 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
     7621 tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa
     7681 acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta
     7741 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
     7801 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
     7861 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
     7921 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
     7981 tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
     8041 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
     8101 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
     8161 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
     8221 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
     8281 ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga
     8341 ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa
     8401 aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa
     8461 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
     8521 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
     8581 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
     8641 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
     8701 acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc
     8761 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
     8821 tacttttgct gcagcttctg catattcacg tttcaatacc caacctgttt ttccatttcc
     8881 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
     8941 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
     9001 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
     9061 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
     9121 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
     9181 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
     9241 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
     9301 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
     9361 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
     9421 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
     9481 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
     9541 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
     9601 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
     9661 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
     9721 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
     9781 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
     9841 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
     9901 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
     9961 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    10021 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    10081 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    10141 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    10201 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    10261 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    10321 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    10381 cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt
    10441 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    10501 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    10561 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    10621 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    10681 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    10741 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    10801 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    10861 ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc
    10921 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    10981 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    11041 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc
    11101 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    11161 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    11221 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    11281 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    11341 tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc
    11401 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    11461 tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    11521 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    11581 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    11641 ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc
    11701 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    11761 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    11821 atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag
    11881 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    11941 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    12001 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    12061 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    12121 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    12181 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    12241 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    12301 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    12361 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    12421 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    12481 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    12541 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    12601 cttatagaac atccgcaatt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    12661 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    12721 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    12781 gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    12841 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    12901 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    12961 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    13021 tccataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    13081 atagataaaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    13141 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    13201 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    13261 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg ggtagatttt
    13321 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccactgc
    13381 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc actctaatct
    13441 ttaaagataa atattaaaat aacgattaat aatattaaca gtctttattt ttcaccacca
    13501 aaaacctatc tttgcatata ctaaatagac gtatatacac agataggttt gtcttgtgaa
    13561 aaataatgat tatccttacc aaaaaatgtt tgtttttcta catgttcgta cttatcaaaa
    13621 aatatgatcc ttattgaaat tactggctat ttatattcca acgacttctt gtgttaattt
    13681 ttgttatctc atagtaaatt atgtttcatt ttcacttaat ttagttgtca cgtgttcgtc
    13741 aattcgtaaa aacggtaaat atataaacac acttgcggct ataattaata tttgtaaaac
    13801 agctgctcgc cagtctccgg ctgttgctaa aaatcccgat ataactggtg gcgtagtcca
    13861 aggaatttgc actactacat gattaattaa tcccacagca gttgcaaaat atgctactgt
    13921 caaagaaaaa atcggtgcaa gcacaaaggg aataatcaga ctgatattaa atacaatagg
    13981 caatccaaaa ataataggtt cgcttatatt gaatatagcg ggagttaccg ataatttagc
    14041 aaagtcctta taatcttttc tacgactgaa gataaatatt gcaatcaata aagcaatcgt
    14101 atttcccgat ccacccatta ccgcaaaggt atctttaaag gccatattga taatatgtgg
    14161 aatttgctct ttgtttgcat aagctgccat attttcttgc atattctgaa tcaaaaacgg
    14221 atctaacaat gaacctgata ttactgcttg atggatacca aatccaaaaa atagattagc
    14281 aatagatgta attaacaaaa atcctggtaa accagttgta attttactta atggctctgt
    14341 aactatcgtg gtaattagct tatttatatc catattaaac aattgaatga ttataaatga
    14401 caataaagca aacgaggtaa tcgtaataat gattgggatt aatacattaa aagactttaa
    14461 aacagcggga ggaatatttc cggatagctg aatctgtagt ttcttattat tagaaagttt
    14521 aataaataat tctgtagccg ctaatccaac aaatatacct acaaacattc ctgaagctcc
    14581 tgtataggat ataggtatca cacccgtcac ttcaacagct ttatccgcat tttcaggagt
    14641 aaacatcatc tgcataggtg ttaatacaac aaatgtcgaa atagcaacta ataccgctgc
    14701 aatatgattt gtataatttc tgcttgcaca catatgataa gatactgctg ctacaactaa
    14761 taatgtagtt atgcttaatg taccattggc aattgaagta ccaatttgtt gtaacgtggt
    14821 caatgtacta gtatcgacaa tttcccccat gaatcctgtt ggttctaaga tcactgaatt
    14881 aataagtgtc acaaatcctg ctaacatcat aaatggcata aatgtagcaa acgcatctcg
    14941 caaagttctt aaatggatct gattccccaa cttagtcgca actataccaa atttttcaaa
    15001 tacaatgtcc ttcctgctac ttttgcccat attttttctc ctttatgctt attgtaatcg
    15061 attacttttt ccacccaaat aatacatcac ttatcaaaaa aagtaaatac ttttaattaa
    15121 atatatatat cttttttagt ataaaaaaat aagctctgaa aaaataattt tcccgagctt
    15181 atttttcaac aaaaacagct tcaaaaacaa agtcttgata taaatgtctc gataccgaat
    15241 actcgaaagg tttaccagac gatagagcaa caactttgtc tatctcaatc ataaattcac
    15301 ctggatctat ttttaatatt tttgcgtcaa attcattcgc agtatctcct ttgatacgta
    15361 tgtgcgaagt ctttatttcc agacccagtt catttgtgat ataagcatat acagatttct
    15421 ttaaatgttt tggttccaag cctggtatga ctgctaatgg catgaatgtt tgctcaatcg
    15481 agtatggttg acaatcaata aaacggcttc gtacaatatt atatacatag tcttttacac
    15541 tgatacttaa tttatcagct acttttttcg atggaagttc aattgagaaa gtatggattt
    15601 ctgaatctac gtgttcttca ccaaccacgc taaacaaacc ttcgtttgga ccaagaactc
    15661 gtgagttatc aattggtgtt ctgacgactt cagtcccata cccacttcta cttcgcaaaa
    15721 ctccttcgga aacaagtaag tctacgcctt tctttactgt taacttgctt gtatgataaa
    15781 taactgccaa ttcatctcct gtaggaagtt tctcaccaat tttatactct ccatttacaa
    15841 tttttttctt tatatctaag taaatttcca tatacttcgc aatttttttc aatggtgacc
    15901 ctccaatcaa aaacatatct tcatctatac tttaacataa ataaaccgat aaaatccaca
    15961 tcaaaattat aca
//
LOCUS       DASJRQ010000063        15938 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJRQ010000063 DASJRQ010000000
VERSION     DASJRQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.063"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            53..1477
                     /locus_tag="U4A80_001856"
     CDS             53..1477
                     /locus_tag="U4A80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1975477.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1776..2483
                     /locus_tag="U4A80_001857"
     CDS             1776..2483
                     /locus_tag="U4A80_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1975478.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2470..2793
                     /locus_tag="U4A80_001858"
     CDS             2470..2793
                     /locus_tag="U4A80_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1975479.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2882..3919)
                     /locus_tag="U4A80_001859"
     CDS             complement(2882..3919)
                     /locus_tag="U4A80_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1975480.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4240..5223)
                     /locus_tag="U4A80_001860"
     CDS             complement(4240..5223)
                     /locus_tag="U4A80_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1975481.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5638..6408)
                     /locus_tag="U4A80_001861"
     CDS             complement(5638..6408)
                     /locus_tag="U4A80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975482.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6401..7336)
                     /locus_tag="U4A80_001862"
     CDS             complement(6401..7336)
                     /locus_tag="U4A80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975483.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7652..9301)
                     /locus_tag="U4A80_001863"
     CDS             complement(7652..9301)
                     /locus_tag="U4A80_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1975484.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9418..12021)
                     /locus_tag="U4A80_001864"
     CDS             complement(9418..12021)
                     /locus_tag="U4A80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1975485.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12033..13082)
                     /locus_tag="U4A80_001865"
     CDS             complement(12033..13082)
                     /locus_tag="U4A80_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN1975486.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13108..13422)
                     /locus_tag="U4A80_001866"
     CDS             complement(13108..13422)
                     /locus_tag="U4A80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1975487.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13438..13884)
                     /locus_tag="U4A80_001867"
     CDS             complement(13438..13884)
                     /locus_tag="U4A80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1975488.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(13885..15807)
                     /locus_tag="U4A80_001868"
     CDS             complement(13885..15807)
                     /locus_tag="U4A80_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN1975489.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4706 a         3240 c         2656 g         5336 t
ORIGIN      
        1 tttaagttgt aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac
       61 tatgaaaaag ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca
      121 agcagaagga aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga
      181 acaaccttac cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca
      241 atataaagaa gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat
      301 ttcttgggca cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc
      361 tttctatcga aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt
      421 atatcatttt gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt
      481 agttgatgct tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg
      541 tcattggttt accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt
      601 tcattatcca aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact
      661 tgcacacgca aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt
      721 tattctaaat ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc
      781 agaaatcgct gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt
      841 ttctaaaaag cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa
      901 agaagatctt gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga
      961 gccacgtcgc gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga
     1021 gtggtttttc gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga
     1081 aatttacgaa aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat
     1141 cgaagcattt atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga
     1201 tacagggcaa ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt
     1261 atggaaggca ataaatgaag gatgtaacat taaggggtat catctttgga catttattga
     1321 ttgctggtca tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac
     1381 tactcaaaaa agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa
     1441 tggatttaga tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt
     1501 gaacacatta gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac
     1561 ttcatttata cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg
     1621 tctggaaatc taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta
     1681 aaattgaagt tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt
     1741 atgaaaattt atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa
     1801 ctgcgatcaa agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg
     1861 gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga
     1921 tctatgctgg ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc
     1981 tctccatcgt tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga
     2041 ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga
     2101 cagatgaaag tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact
     2161 ttcgttggat gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg
     2221 ttggcgcact tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca
     2281 tcgtcgctat gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta
     2341 aacgattaca gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt
     2401 ttatcccaag taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt
     2461 ggatcaaaca tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca
     2521 acttggcttt ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg
     2581 ttgcttgagt atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt
     2641 ctatttaccc aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca
     2701 gttcctacac ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga
     2761 attctttccc tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat
     2821 tactgtcatg cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg
     2881 cttatccgtt gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt
     2941 ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg
     3001 tactgcggat aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg
     3061 tttcgatttc atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag
     3121 attggttttt ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat
     3181 gtccaaatgg tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata
     3241 cagcgacata ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat
     3301 gataatcgat tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg
     3361 cagtcgcgta gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc
     3421 ccccagctcc gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca
     3481 gaccaaattt ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg
     3541 cttcttcatc tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt
     3601 attcttgatt gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag
     3661 aaaatttttg cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga
     3721 tacgtaaagc atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag
     3781 atactgcgtt gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt
     3841 catcaataaa gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca
     3901 ttaattcctt ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac
     3961 agttttcatt tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt
     4021 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
     4081 tttgtcacaa cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa
     4141 acgacaaaat atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
     4201 tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt
     4261 tggatgttct ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt
     4321 catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa
     4381 acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag
     4441 ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa
     4501 tgcataagga ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc
     4561 ggccaattct tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg
     4621 cggagcgatt tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac
     4681 tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag
     4741 taggtgactg atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac
     4801 gctgacaggc aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg
     4861 aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt
     4921 gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc
     4981 tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct
     5041 tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt
     5101 aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac
     5161 atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt
     5221 catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat
     5281 tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga
     5341 ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt
     5401 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt
     5461 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa
     5521 cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta
     5581 ttttcccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta
     5641 acttctcaat cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat
     5701 gaatagcaca agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc
     5761 gtaacggaaa ccggagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa
     5821 tggcggcaac atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac
     5881 aaatgtaggc acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc
     5941 tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata
     6001 cacatgaagt ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact
     6061 gcgacccact ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac
     6121 caagatttct tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc
     6181 atacgaacta gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga
     6241 aaaaccaccc atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa
     6301 ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt
     6361 ttccttgata gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg
     6421 tatgattatt ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc
     6481 tcatagaaag aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt
     6541 gatttcgttc aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt
     6601 caatcacagg tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt
     6661 tggatagaag ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga
     6721 tgttgcgaca aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac
     6781 cattttcgtt caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc
     6841 ctgctgttgg ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca
     6901 tcagtcttcg cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa
     6961 gattggactg cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt
     7021 agtagcctgc ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact
     7081 cttgcgggac taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac
     7141 cgaagacatt cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg
     7201 tagacttccc agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa
     7261 gatcgattcc tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt
     7321 caagcgcatt tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg
     7381 gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg
     7441 aaaatctcct aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca
     7501 aatcgaagat aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa
     7561 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt
     7621 ttgatccaat aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt
     7681 catcaaataa cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa
     7741 ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa
     7801 aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct
     7861 ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg
     7921 caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta
     7981 aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt
     8041 tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg
     8101 ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga
     8161 tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg
     8221 cattggaacc cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt
     8281 ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc
     8341 aagtgatggg catataagta cggtaattca atattgcagc ggctacttct aattctccca
     8401 gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat
     8461 gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt
     8521 cactcccact cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag
     8581 tttgctgttt cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg
     8641 aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca
     8701 ctagataata gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt
     8761 gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac
     8821 ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat
     8881 ctttttttcc taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct
     8941 gataaaaagc aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac
     9001 gttgatacaa cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt
     9061 cgaacatatc caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact
     9121 tcttctccgt gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag
     9181 gaattttttc ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta
     9241 aagcaactaa taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca
     9301 tatccctctc cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa
     9361 gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta
     9421 tctctcattt atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt
     9481 attctccagt gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc
     9541 ttgttctgtt ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata
     9601 aatcgatgat acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc
     9661 aagttcattt tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt
     9721 ctgcttttga taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa
     9781 ctcattaaat cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc
     9841 ttgagctagc ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg
     9901 cccaggtcgc caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat
     9961 gattaattga ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata
    10021 atgatcattt tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc
    10081 taccatagtt tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt
    10141 ttgaatttgt gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg
    10201 atgcgagtaa acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag
    10261 actgaacgat atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt
    10321 taaatcttta ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt
    10381 atatacttta gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata
    10441 atcatacgta tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct
    10501 atctccaact atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact
    10561 tatctcagtt ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc
    10621 aacaacagtg tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata
    10681 agctggttca tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc
    10741 tggaaaataa acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt
    10801 tgaatcagct actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa
    10861 aacaaggact tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt
    10921 ttcgattccg tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac
    10981 attatctgaa acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca
    11041 taaccgacga agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag
    11101 cggctcaact cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt
    11161 tcttactgag ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt
    11221 atattcaggt aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat
    11281 aatattgtgg aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat
    11341 cattgacttt tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg
    11401 aagcatttca actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc
    11461 gtctaaggtc atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt
    11521 atctcccaat cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc
    11581 tcgccaacac atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt
    11641 gaacccaaac gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt
    11701 atcactgatt ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc
    11761 tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac
    11821 cttaagcttt tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa
    11881 acaaaagttt actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc
    11941 agacaatacg atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg
    12001 aattatatgt gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact
    12061 tgttgttctt ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact
    12121 cccccaataa caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata
    12181 aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact
    12241 gcagtgccca ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc
    12301 gtcccttcag taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc
    12361 gattcatcat aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata
    12421 gcaattccta ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc
    12481 ccaaaagcat atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct
    12541 aaaatagccg acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt
    12601 cctgttgtca ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact
    12661 aatgtagtaa caaaaggaat cagcataatt gattttagac taataacttg catcggtact
    12721 tttacttttt tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca
    12781 ataaatccca taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg
    12841 ggacggtcag caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat
    12901 cccgtctgac caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct
    12961 gtgccaccaa aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc
    13021 atataggaaa ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc
    13081 attagtaact cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat
    13141 cgtctcttca ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc
    13201 aaagcgacct cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga
    13261 aacatccgaa attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat
    13321 atctaaccct ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc
    13381 aatccctgcg atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta
    13441 aaatgataga gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa
    13501 ctcgtcatcc atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt
    13561 atcagtgtct tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc
    13621 ccattcaaca gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct
    13681 tttgccatga ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc
    13741 aaatacgtct ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt
    13801 cgataatttt tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt
    13861 tatattgatt ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc
    13921 aaacgacgaa ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa
    13981 tgtttcataa attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac
    14041 agaatgaaca agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac
    14101 tggctttttt ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga
    14161 agatagtttt tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt
    14221 taccaatttt ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt
    14281 aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa
    14341 ctgtagatac tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat
    14401 aacaggaaaa tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt
    14461 tctatcttgt agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc
    14521 gttatttatt ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat
    14581 cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta
    14641 agcgatttca tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc
    14701 tacacttatc ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata
    14761 gatatttact cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt
    14821 gatcaattct tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc
    14881 aacacccata tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt
    14941 ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga
    15001 aaatgctttt tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga
    15061 attattacta cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg
    15121 gatagctaaa gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg
    15181 attaagcaat tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc
    15241 aatgaaattg atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc
    15301 ccaataatag ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat
    15361 gcgcacgcca tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac
    15421 atctttcatt gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg
    15481 aatctttaga gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc
    15541 cggagagtct gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt
    15601 tccaataagc gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg
    15661 gattatttct ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg
    15721 ttctgataat tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat
    15781 ttgccgttcg ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac
    15841 aattattatc tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata
    15901 ttcttgatta tttcaaatca ataaaaaagc ctgcaata
//
LOCUS       DASJRQ010000064        15745 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJRQ010000064 DASJRQ010000000
VERSION     DASJRQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.064"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..726)
                     /locus_tag="U4A80_001869"
     CDS             complement(28..726)
                     /locus_tag="U4A80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1975490.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="U4A80_001870"
     CDS             complement(719..2071)
                     /locus_tag="U4A80_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1975491.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="U4A80_001871"
     CDS             complement(2294..3694)
                     /locus_tag="U4A80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1975492.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="U4A80_001872"
     CDS             3855..4592
                     /locus_tag="U4A80_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1975493.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="U4A80_001873"
     CDS             4717..5196
                     /locus_tag="U4A80_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1975494.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="U4A80_001874"
     CDS             5193..5960
                     /locus_tag="U4A80_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975495.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="U4A80_001875"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="U4A80_001875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1975496.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="U4A80_001876"
     CDS             complement(6863..7465)
                     /locus_tag="U4A80_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1975497.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="U4A80_001877"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="U4A80_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="U4A80_001878"
     CDS             8146..8730
                     /locus_tag="U4A80_001878"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1975498.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="U4A80_001879"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="U4A80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1975499.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="U4A80_001880"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="U4A80_001880"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1975500.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="U4A80_001881"
     CDS             10631..10810
                     /locus_tag="U4A80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975501.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="U4A80_001882"
     CDS             10832..11857
                     /locus_tag="U4A80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1975502.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="U4A80_001883"
     CDS             12032..13276
                     /locus_tag="U4A80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1975503.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="U4A80_001884"
     CDS             13429..14376
                     /locus_tag="U4A80_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975504.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="U4A80_001885"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="U4A80_001885"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1975505.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="U4A80_001886"
     CDS             complement(15278..15520)
                     /locus_tag="U4A80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975506.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="U4A80_001887"
     tRNA            15641..15713
                     /locus_tag="U4A80_001887"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3196 g         4377 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttt
//
LOCUS       DASJRQ010000065        15563 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJRQ010000065 DASJRQ010000000
VERSION     DASJRQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.065"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1018..1359)
                     /locus_tag="U4A80_001888"
     CDS             complement(1018..1359)
                     /locus_tag="U4A80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1975507.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="U4A80_001889"
     CDS             complement(1359..1589)
                     /locus_tag="U4A80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1975508.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1876..2214
                     /locus_tag="U4A80_001890"
     CDS             1876..2214
                     /locus_tag="U4A80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975509.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2419..2658)
                     /locus_tag="U4A80_001891"
     CDS             complement(2419..2658)
                     /locus_tag="U4A80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1975510.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2634..2981)
                     /locus_tag="U4A80_001892"
     CDS             complement(2634..2981)
                     /locus_tag="U4A80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1975511.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3713..4294)
                     /locus_tag="U4A80_001893"
     CDS             complement(3713..4294)
                     /locus_tag="U4A80_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1975512.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4477..5103)
                     /locus_tag="U4A80_001894"
     CDS             complement(4477..5103)
                     /locus_tag="U4A80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1975513.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5125..6456)
                     /locus_tag="U4A80_001895"
     CDS             complement(5125..6456)
                     /locus_tag="U4A80_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1975514.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6611..7012)
                     /locus_tag="U4A80_001896"
     CDS             complement(6611..7012)
                     /locus_tag="U4A80_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1975515.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7098..7304)
                     /locus_tag="U4A80_001897"
     CDS             complement(7098..7304)
                     /locus_tag="U4A80_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975516.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7399..8901)
                     /locus_tag="U4A80_001898"
     CDS             complement(7399..8901)
                     /locus_tag="U4A80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1975517.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8919..9374)
                     /gene="argR"
                     /locus_tag="U4A80_001899"
     CDS             complement(8919..9374)
                     /gene="argR"
                     /locus_tag="U4A80_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1975518.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9472..10089)
                     /locus_tag="U4A80_001900"
     CDS             complement(9472..10089)
                     /locus_tag="U4A80_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1975519.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10310..11683
                     /locus_tag="U4A80_001901"
     CDS             10310..11683
                     /locus_tag="U4A80_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1975520.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11758..12870
                     /locus_tag="U4A80_001902"
     CDS             11758..12870
                     /locus_tag="U4A80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1975521.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12942..14579)
                     /locus_tag="U4A80_001903"
     CDS             complement(12942..14579)
                     /locus_tag="U4A80_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1975522.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14747..15355)
                     /locus_tag="U4A80_001904"
     CDS             complement(14747..15355)
                     /locus_tag="U4A80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1975523.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4324 a         3180 c         2704 g         5355 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
     1741 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
     1801 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
     1861 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
     1921 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
     1981 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
     2041 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
     2101 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
     2161 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
     2221 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
     2281 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
     2341 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
     2401 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
     2461 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
     2521 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
     2581 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
     2641 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
     2701 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     2761 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     2821 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     2881 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
     2941 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3001 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
     3061 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3121 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3181 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
     3241 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
     3301 cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     3361 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc
     3421 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa
     3481 tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     3541 tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca
     3601 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
     3661 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
     3721 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
     3781 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
     3841 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
     3901 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
     3961 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
     4021 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
     4081 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
     4141 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
     4201 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
     4261 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
     4321 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
     4381 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
     4441 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
     4501 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
     4561 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     4621 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     4681 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     4741 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     4801 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     4861 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     4921 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     4981 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     5041 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     5101 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     5161 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     5221 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     5281 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     5341 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     5401 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     5461 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     5521 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     5581 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     5641 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     5701 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     5761 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     5821 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     5881 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     5941 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     6001 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     6061 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     6121 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     6181 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     6241 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     6301 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     6361 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     6421 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     6481 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     6541 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     6601 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     6661 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     6721 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     6781 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     6841 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     6901 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     6961 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     7021 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     7081 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     7141 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     7201 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     7261 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     7321 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     7381 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     7441 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     7501 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     7561 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     7621 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     7681 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     7741 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     7801 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     7861 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     7921 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     7981 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     8041 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     8101 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     8161 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     8221 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     8281 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     8341 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     8401 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     8461 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     8521 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     8581 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     8641 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     8701 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     8761 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     8821 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     8881 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     8941 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     9001 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     9061 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     9121 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     9181 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     9241 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     9301 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     9361 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     9421 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     9481 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     9541 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     9601 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     9661 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     9721 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     9781 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     9841 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     9901 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     9961 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
    10021 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
    10081 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
    10141 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
    10201 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
    10261 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
    10321 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
    10381 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
    10441 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
    10501 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
    10561 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
    10621 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
    10681 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
    10741 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
    10801 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
    10861 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
    10921 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
    10981 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
    11041 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
    11101 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
    11161 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
    11221 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
    11281 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
    11341 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
    11401 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
    11461 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
    11521 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
    11581 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
    11641 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
    11701 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
    11761 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
    11821 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
    11881 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
    11941 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
    12001 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
    12061 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
    12121 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
    12181 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
    12241 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
    12301 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
    12361 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
    12421 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
    12481 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
    12541 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
    12601 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
    12661 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
    12721 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
    12781 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
    12841 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
    12901 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
    12961 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
    13021 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
    13081 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
    13141 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
    13201 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
    13261 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
    13321 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
    13381 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
    13441 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
    13501 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
    13561 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    13621 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    13681 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    13741 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    13801 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    13861 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    13921 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    13981 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    14041 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    14101 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    14161 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    14221 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    14281 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    14341 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    14401 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    14461 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    14521 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    14581 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    14641 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    14701 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    14761 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    14821 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    14881 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    14941 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    15001 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    15061 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    15121 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    15181 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    15241 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    15301 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    15361 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    15421 tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt
    15481 aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag
    15541 taatcgtttt tctatgtttt ttt
//
LOCUS       DASJRQ010000066        15489 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJRQ010000066 DASJRQ010000000
VERSION     DASJRQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.066"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..577
                     /locus_tag="U4A80_001905"
     CDS             191..577
                     /locus_tag="U4A80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1975524.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            671..862
                     /locus_tag="U4A80_001906"
     CDS             671..862
                     /locus_tag="U4A80_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975525.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1376..1708
                     /locus_tag="U4A80_001907"
     CDS             1376..1708
                     /locus_tag="U4A80_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1975526.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2004..2159
                     /locus_tag="U4A80_001908"
     CDS             2004..2159
                     /locus_tag="U4A80_001908"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1975527.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2435..3775
                     /locus_tag="U4A80_001909"
                     /pseudo
     CDS             2435..3775
                     /locus_tag="U4A80_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4016..5632)
                     /locus_tag="U4A80_001910"
     CDS             complement(4016..5632)
                     /locus_tag="U4A80_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1975528.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5750..5968
                     /locus_tag="U4A80_001911"
     CDS             5750..5968
                     /locus_tag="U4A80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975529.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6163..6624
                     /locus_tag="U4A80_001912"
     CDS             6163..6624
                     /locus_tag="U4A80_001912"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1975530.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6817..7056)
                     /locus_tag="U4A80_001913"
     CDS             complement(6817..7056)
                     /locus_tag="U4A80_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975531.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7080..8603)
                     /locus_tag="U4A80_001914"
     CDS             complement(7080..8603)
                     /locus_tag="U4A80_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1975532.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8621..8743)
                     /locus_tag="U4A80_001915"
     CDS             complement(8621..8743)
                     /locus_tag="U4A80_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1975533.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8773..9756)
                     /locus_tag="U4A80_001916"
     CDS             complement(8773..9756)
                     /locus_tag="U4A80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1975534.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9780..9929)
                     /gene="rpmG"
                     /locus_tag="U4A80_001917"
     CDS             complement(9780..9929)
                     /gene="rpmG"
                     /locus_tag="U4A80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1975535.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9950..10327)
                     /locus_tag="U4A80_001918"
     CDS             complement(9950..10327)
                     /locus_tag="U4A80_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975536.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10359..10538)
                     /gene="rpmF"
                     /locus_tag="U4A80_001919"
     CDS             complement(10359..10538)
                     /gene="rpmF"
                     /locus_tag="U4A80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1975537.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10553..10822)
                     /gene="rpsN"
                     /locus_tag="U4A80_001920"
     CDS             complement(10553..10822)
                     /gene="rpsN"
                     /locus_tag="U4A80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1975538.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11345..13111)
                     /locus_tag="U4A80_001921"
     CDS             complement(11345..13111)
                     /locus_tag="U4A80_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975539.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13071..14825)
                     /locus_tag="U4A80_001922"
     CDS             complement(13071..14825)
                     /locus_tag="U4A80_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975540.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14935..>15489)
                     /locus_tag="U4A80_001923"
     CDS             complement(14935..>15489)
                     /locus_tag="U4A80_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1975541.1"
                     /translation="YIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGII
                     FVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRA
                     IPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIIS
                     VLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5001 a         2973 c         2365 g         5150 t
ORIGIN      
        1 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
       61 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      121 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      181 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      241 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      301 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
      361 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
      421 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
      481 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
      541 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
      601 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
      661 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
      721 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
      781 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
      841 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
      901 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
      961 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     1021 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     1081 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     1141 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     1201 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     1261 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     1321 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     1381 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     1441 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     1501 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     1561 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     1621 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     1681 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     1741 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     1801 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     1861 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     1921 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     1981 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     2041 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     2101 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     2161 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     2221 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     2281 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     2341 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     2401 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     2461 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     2521 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     2581 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa tagggctgga aaaaagtatc
     2641 catcttattg aatgatgtca gtaactacaa gaccattctg cggattaaca aacagcgctt
     2701 caaatgtaaa cactgtggaa agactttttt agcagaagat tcagttagtg atcgtcgctg
     2761 ctccattgct cgcagagtca aacaagccat tcttgagcta ttaagcgaac ctctttctat
     2821 gtctttgatc gcacgtatga agcacatttc tccgaccact gtcattcgaa tacttcgcag
     2881 ccttcggcct aaaaccattt ctttgagcca gctgttacca gaagtggtgt gtttcgatga
     2941 attcaaatca gtgaaaaatg tctcgggagc catgagcttt gtcatgatgg acggcaagac
     3001 ccatcaactg attgatattg tggaaaatcg gcaactcaat cctttacgcg actatttctt
     3061 gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg gtttcagatt tctatacacc
     3121 ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga atcgtcgccg atcgctttca
     3181 tattagccaa cacattggtc gggcttttac caaccaccga attcaagtaa tgaagacctt
     3241 taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac tggaaactac ttcaaaagaa
     3301 tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg tcatttcgag atcatctgac
     3361 agaagcagag atagtcgatc ggctattatc ttatgatgat tccttaaagc gaggctacga
     3421 agtctaccag gattttcttt ccgttatcag aagacaagat gttcctgaat tcgtcgcgct
     3481 cctaaaagaa gactacaagg agttgccaga gcactaccaa ccagtgttca cgaccttcaa
     3541 gaaataccaa acagaaatca aacgagcgtt gcgcgttccc tattccaacg gccctataga
     3601 atgtttgaat aaccatatca aagtactaaa acgaattgcc tatggctata gaaactttca
     3661 aaactaccga gaacgaatct ttttatatcg aggaaaatat tttaagaaaa ctaagaatac
     3721 tacccaactg actaaagcca gaacaacaac acggctagac aagatagctg tctagccgtg
     3781 tgagtatctc gattattcta ttgaatttct tcaccaacac tcattgacga agagccaaaa
     3841 taagtaatct aaccgactgt taactaaacc attgcaggtc ccgttaaaga tacttgtgcc
     3901 tgttgtcacc gcttcttttt cttgtccaca agcgcttaac acacttaata aagcccctgc
     3961 tattattaat aataatatct ttttcttatt aaaaacctcc tcatctattt ccagattagt
     4021 aaatgtagta atctgtgctt tcttttttct caatactaat aactaactta tggggtaaac
     4081 aaatactcgt ttgaccgggt tttgagatcc atccagttct aaccgcgatt tgatcaggac
     4141 tattatcttc tttaactcga attcttccat ctttaatctc aacaatatta tattgtccct
     4201 tcgcaggatg ataattcacg agtttatgat tgatcttatc caaatcgaag cgatccattt
     4261 ccttgccatc aattcgaacg atggcatatt tgatagcatc ttcagttgtt tcattctcct
     4321 tttctataga gaatacaaat aagggaataa aagaggtaat aatcaaaatg ccaataatta
     4381 tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt cattaacgat atctggctaa
     4441 acatattaga ctttttccat ggaaatggtg aaatttttcg gaaattatct gcattaagag
     4501 aatcagttaa acaaccctca agttgttcct taatacattg ttgatccatc ttcataccaa
     4561 taaaaactag tttattaatg cgttcaccaa acaacggatg ccaggcttcc ttcaattcgg
     4621 gatttttttg tataatcatt tgacactcaa ttttaggtaa actagctacc caataattta
     4681 atgattcaat acttttatag ctacccgatg tttcaaagat cgtcgaaaaa tcactatgtt
     4741 gggcaaacca aagaaaccct tttgctcttg taatattgcc aggaaaattt ttatccgtaa
     4801 gatgggcaaa tttttgtgcg tcaaggagta accgatttcg atagataaaa cttgagatac
     4861 catattcttc tgtttcagga atatgatttt gatagccaag ctctaactct ttgaaccagc
     4921 ccactgaata ggaagtctca tctatattaa aaagatttgt atttagaatt ttcgataagt
     4981 ctacttcacc tttaattgtc ctaattattt tggcattgga ctgtaactta gcaattagac
     5041 cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact attatttaaa agtaatatgt
     5101 tacaacactc gaattgatca attaacaagc cacgaatatc ctgttcttct tcaactacat
     5161 taccttcagc ttttctttcc tgaatctttt cacccgtttc gaaattatcc caaaaacgtt
     5221 ttacatccac tactataata atggcaccaa tctctaatcg ttgggtcaaa tcaatgtcta
     5281 aagggttttt agctaatata attgcttgaa taattggaat tggtttgctg attcctgaag
     5341 cttcaataac aatttgattc aagtcagaaa attgcactaa ttgatctagt tcaacaatca
     5401 aatcttcgcg taatgtacaa caaatgcatc cattcggcat cgaaacaagt ttttctccag
     5461 ttcttttaaa acctttcttg tcgactaata ccccatcaac atttacttcc cctatgtcat
     5521 ttacaatgac agcaatttcc tttccgtgat caccgtacag aatatgattt agaagtatcg
     5581 tttttcctga accaagatag ccagttaaga ttgtaatagg tatttttttc atagggaacc
     5641 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     5701 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     5761 tcgtcatctt actatactcg aacgagaaag aatctttctg cttcacgaag aaggattttc
     5821 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     5881 aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     5941 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     6001 ttatttgtcg attttcagtg gtcgccagag gaaatctcga atcgtttgaa gcatgaacat
     6061 atggactttt caattagttt tatcacgatc taacgtgcaa tttgcaatga tcttttcgat
     6121 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     6181 acaagacata caaaaaatca tgtcgagtct cgtggaaaga tttgccttac tcattcgatc
     6241 catggccgtc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     6301 gtagctggca aaactggtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     6361 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     6421 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     6481 gaattctcca atcatttcgc agttacagaa gcattgaata atatgccctt ttattttcca
     6541 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     6601 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     6661 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     6721 ttgataaagt gttgcgcttg atttgacaat tcaagacata ataaaaagca gatcaaatcc
     6781 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     6841 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     6901 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     6961 tgtactccca tttaatctca tttgctttgc aaatcgataa cgccgataag catcaatatt
     7021 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa aattgaattt
     7081 taatgagcca tcatttcttg agcaatttca tgtttagaca atgaagctgg atagaaagta
     7141 ggccaattat gcaattcatt atagagtttt tcttgacttt ctccaccaaa gaaaatatgg
     7201 aaatgctctg ccttacttgg tgcaattcca tgatcactaa attgaatata tttaaaacga
     7261 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     7321 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     7381 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     7441 taataatctt tgtactcggc agctgtcatg tctttattga tttttgcttt gtaatcgaaa
     7501 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     7561 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     7621 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttttct
     7681 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     7741 tttgtaagac tttcaagagg attcagtact tccaatttta tcccagcttc gttggccaaa
     7801 gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     7861 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     7921 ccagcaatag gaacttgttt caatccataa tccaaagcta aatatccaaa ggctgcgtgt
     7981 tgcgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     8041 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     8101 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     8161 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     8221 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     8281 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     8341 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     8401 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     8461 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     8521 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     8581 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     8641 tatttcgttt atgttctttg ataattttta atgccctttt tctagtttta atattcgggc
     8701 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccccaat
     8761 cactacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     8821 ttcatccaac atttttccga ttagaataat agtggtttgg tctttttgtc gagtggtgta
     8881 ttgaaaatta acctgttcgt ttacctcttg catggcaact ataaaatcct gctcttgtaa
     8941 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccagtcaat
     9001 ccattggagt aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     9061 ctcatgtact tgatggtgtt gatgatagta tttgccatta tagtgatttt ctgatgaatc
     9121 actttcaaaa actaagggat gattaaattt tttcaaatta taaaaatcct ttttttgaat
     9181 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     9241 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     9301 tgccatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     9361 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     9421 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     9481 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     9541 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     9601 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     9661 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     9721 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     9781 tatttcactt ctttaaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     9841 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     9901 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     9961 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
    10021 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcgata cattgaaata
    10081 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
    10141 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
    10201 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
    10261 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
    10321 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
    10381 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
    10441 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
    10501 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
    10561 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
    10621 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
    10681 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
    10741 ttaattcctg acgtttatct gcatactttt caatcatcgc catttgtttc tttgctttag
    10801 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacacctc
    10861 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
    10921 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
    10981 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaggctctg aaaaataaga
    11041 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    11101 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    11161 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
    11221 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
    11281 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
    11341 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
    11401 ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta ccttgatcaa
    11461 ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
    11521 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
    11581 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
    11641 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
    11701 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
    11761 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
    11821 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
    11881 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
    11941 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
    12001 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
    12061 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
    12121 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
    12181 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
    12241 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
    12301 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
    12361 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
    12421 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
    12481 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
    12541 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
    12601 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
    12661 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
    12721 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
    12781 gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct ttataagtac
    12841 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacaa
    12901 acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat tcgtctatca
    12961 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa ctagaaatag
    13021 aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt ttataaaaac
    13081 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
    13141 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
    13201 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
    13261 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
    13321 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
    13381 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
    13441 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
    13501 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
    13561 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
    13621 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
    13681 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
    13741 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
    13801 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    13861 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    13921 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    13981 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    14041 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    14101 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    14161 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattccg
    14221 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    14281 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    14341 agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa aacaggtgtt
    14401 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    14461 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    14521 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    14581 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    14641 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agactgaaga
    14701 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    14761 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    14821 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    14881 ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta cgttctattt
    14941 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    15001 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    15061 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    15121 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    15181 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    15241 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    15301 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    15361 gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg ccccaccaat
    15421 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    15481 aaaaatata
//
LOCUS       DASJRQ010000067        14586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJRQ010000067 DASJRQ010000000
VERSION     DASJRQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.067"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(310..876)
                     /locus_tag="U4A80_001924"
     CDS             complement(310..876)
                     /locus_tag="U4A80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1975542.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(876..1832)
                     /locus_tag="U4A80_001925"
     CDS             complement(876..1832)
                     /locus_tag="U4A80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1975543.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1832..2488)
                     /locus_tag="U4A80_001926"
     CDS             complement(1832..2488)
                     /locus_tag="U4A80_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1975544.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2858..4876
                     /locus_tag="U4A80_001927"
     CDS             2858..4876
                     /locus_tag="U4A80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1975545.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4946..5152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5303..7717
                     /locus_tag="U4A80_001928"
     CDS             5303..7717
                     /locus_tag="U4A80_001928"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1975546.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8065..9618
                     /locus_tag="U4A80_001929"
     CDS             8065..9618
                     /locus_tag="U4A80_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1975547.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9999..11783)
                     /locus_tag="U4A80_001930"
     CDS             complement(9999..11783)
                     /locus_tag="U4A80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975548.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11798..13519)
                     /locus_tag="U4A80_001931"
     CDS             complement(11798..13519)
                     /locus_tag="U4A80_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975549.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13619..14380
                     /locus_tag="U4A80_001932"
     CDS             13619..14380
                     /locus_tag="U4A80_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1975550.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4727 a         2703 c         2897 g         4259 t
ORIGIN      
        1 tataaaatct tttaaaaatg cttatatatc aatgtttttg ccattctttt atatatctgt
       61 tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa
      121 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgatt
      181 tggttggact tattaccgca gatataagct tttgagcacc gtgaataaag aggttatgaa
      241 atcaacgctc taacctgagt attagagaaa aaaacgcaaa acatatcatt tatgttttgc
      301 gttttttatt tatctttttt tctcaataca atataaaata ggcggctggt tcttttggtt
      361 gataaaccga taattcagca cattatatgt ttcctgtggc agtttggaac aaaaagaatc
      421 aaccatatcc agttcttttt ctccaccttc atgaccatag tagacgacaa ggatcaatcg
      481 tccgcgaggc acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag tctctggcat
      541 agtgatgatt gctttatcac tttttgggag ataccctaga ttgaaaatcc ctgctttgag
      601 attttgttgt tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac
      661 acgttcttct aactgacgct gttctagcct ttcacgagta gcatcgattg cttgttgctg
      721 gatatcaaaa ctatagacat gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc
      781 gtggccattg cccatcgttg catctacgac gtgatcccct ggttgcaaga tttcttgcag
      841 taatgtatgg ctgaaatgca aagcagtttg caacatcaga cgctcacctt tttccgtaca
      901 tcatattttc cttggaagct atctctgcga agaagttctt catcaattgc attcaagact
      961 tcccattttt tcaagctcca cattggtcca atcaaggaat cccacggcgc atcccctgtc
     1021 aaacgatgga tgatgatctc ttttggtatc atttccagct ggtcgcaaat gacattgaca
     1081 tagtcaggac ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt
     1141 ttgcgcatca gatgcatcaa atggagcttg attccttgga tatctgaatc taaaatggtg
     1201 cgtctgacat tttctttcat catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc
     1261 gtacaaacac ggatattatg tttgcgtaat ttagccacgc catctaaata agtttgataa
     1321 tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct
     1381 acccataaat acatccgctc gttcagttca gcaaggtaat caactacttc atctggcaga
     1441 caatccggtc gtgtaccgac tgataatcct acgacacctg gtaaattgac tacttgctca
     1501 aatcgttctt tgattatttc aactggcgca tgggtgtttg taaaattttg gaaataaacg
     1561 atgtattgtt ggacatgagg ccatttttga tgcatcatgt cgatttcttt ctggaattgg
     1621 attggaagag ggtcttcagg tgcaacgatc atgtctcccg aaccagatac actgcaaaaa
     1681 gtacaaccgc catgggcaac agtcccatct cgattcggac agtcaaatcc tccatcgatc
     1741 ggcaccttga aaattttccc gccaaactca tttcgtaaag cgtaattcca agaatgatat
     1801 cttttatttc cttctgaata aggaaaagtc attaaaagtc cttctttctg taagagttat
     1861 ctatttgttt tcttttttct ctatacactg gataagtcag gcacagccaa gaaagactga
     1921 gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc cagataaatg cggcttatcc
     1981 cgacacttac tataataaac atcatcagca attgaagcaa tgtgcgtaag gttctttttt
     2041 gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag aatcatactg cctgctgcat
     2101 gtccactagg gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc
     2161 gcatgaaaaa tagtttgatc accgggttga tgattccagc aattacaata aatccagaaa
     2221 cgaaccaaaa tgtttctgcc ctttttttct tataccacat ccataaagcc gcacaagcaa
     2281 ataaaatgat gacggtaaga ggattaccga atttagtcat aaagagttgg tagctgttca
     2341 gatcaggata agaagctctg acaatgcctg taaaccactg atcgaaacca gaaagccacg
     2401 acagatggaa ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag aagctaccag
     2461 ccacctgaaa tgatgtttta tatttcatac aattcctcct ttgaagcaat gacttattat
     2521 agcatttttg gtccagcgaa aaaaacggta aagagataaa accaatgtat gattcctctt
     2581 tgaataacca atattcttat aaaaaaaata actgtaatcg gaaagacatt ttttggtaat
     2641 gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga aatcctttta aacaagaacg
     2701 tagacgtagg aagaacttga aaatgaacag aagttgttca ttacggtaaa ttacaatatg
     2761 attacaaaaa acaacaaagc tcttaaataa cttttaataa atattaagag aagttataat
     2821 tattaataat agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga
     2881 acggagacgt ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt
     2941 gggaactgcg atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca
     3001 agcggatgaa acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga
     3061 aattgctact tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat
     3121 cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta
     3181 taatcttttt gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga
     3241 atttttaaat aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc
     3301 tgaatctttc aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta
     3361 ctatgccggt acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac
     3421 agggcgttat gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta
     3481 cggattgacc caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg
     3541 tggcaataat actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa
     3601 ctcaggttca gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat
     3661 ctcaaataaa ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa
     3721 tttcatttat ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac
     3781 gaataattcc ggtaacaata ccaattcttc aggaaatgct ggtacatcaa acggaggaca
     3841 aacaactggt gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga
     3901 tcacggaatt acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta
     3961 tccaggacaa cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg
     4021 ctcaaatact gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc
     4081 ttcaggaacg aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata
     4141 cggaattacg atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc
     4201 aggacaacaa ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg
     4261 ttcgtcaacc aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg
     4321 cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac
     4381 gatggatcag ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca
     4441 attgatcgtt aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc
     4501 gaaaccaaat acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat
     4561 gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat
     4621 ggatcagtta atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt
     4681 gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac
     4741 ctatactgtg aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat
     4801 ggctcaacta atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt
     4861 gattgtagct aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt
     4921 tgttactata aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga
     4981 agtttcagag agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga
     5041 gttgcccttg ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag
     5101 gccttttcac taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca
     5161 atctttttat aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa
     5221 ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt
     5281 caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca
     5341 aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt
     5401 ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga
     5461 aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct
     5521 gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg
     5581 aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc
     5641 tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa
     5701 atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt
     5761 agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg
     5821 gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa
     5881 aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag
     5941 cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa
     6001 agtggctggt acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg
     6061 tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga
     6121 acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag
     6181 aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt
     6241 taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg
     6301 agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg
     6361 actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga
     6421 tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa
     6481 aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg
     6541 tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga
     6601 ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa
     6661 aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa
     6721 tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg
     6781 ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc
     6841 agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca
     6901 tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt
     6961 tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa
     7021 caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac
     7081 ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat
     7141 tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt
     7201 gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct
     7261 tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt
     7321 caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct
     7381 gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg
     7441 tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac
     7501 atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa
     7561 agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc
     7621 attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc
     7681 ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact
     7741 gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt
     7801 gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca
     7861 cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt
     7921 tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca
     7981 aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat
     8041 gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga agtattatca
     8101 gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt
     8161 ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc
     8221 catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac
     8281 ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt
     8341 tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa
     8401 cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact
     8461 tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt
     8521 gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat
     8581 atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac
     8641 ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca
     8701 atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac
     8761 atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct
     8821 gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca
     8881 gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt
     8941 gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt
     9001 tattggatgg acaacacata tgatatttcc gccgatttct catggtttat ctattcttct
     9061 ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt
     9121 atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt
     9181 ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact
     9241 atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa
     9301 aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat
     9361 acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag
     9421 acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg
     9481 ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc
     9541 tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa
     9601 gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa
     9661 gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag
     9721 ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg
     9781 tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa
     9841 gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta
     9901 ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac
     9961 taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta
    10021 ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg
    10081 acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg
    10141 tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat
    10201 gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc
    10261 gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt
    10321 tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt
    10381 gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata
    10441 ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga
    10501 cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta
    10561 ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga
    10621 ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt
    10681 ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct
    10741 aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc
    10801 ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt
    10861 ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca
    10921 cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg
    10981 aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat
    11041 tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg
    11101 accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac
    11161 tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg
    11221 ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga
    11281 ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact
    11341 tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg
    11401 tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat
    11461 gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt
    11521 aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg
    11581 aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt
    11641 cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat
    11701 ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg
    11761 ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa
    11821 actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt
    11881 ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa
    11941 caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg
    12001 ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca
    12061 acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag
    12121 caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct
    12181 tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta
    12241 aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct
    12301 gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt
    12361 cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga
    12421 tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga
    12481 tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg
    12541 gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt
    12601 ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata gctgcgatca
    12661 tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta
    12721 atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca
    12781 tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat
    12841 tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg
    12901 cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt
    12961 ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata
    13021 cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca
    13081 tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa
    13141 tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa
    13201 aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt
    13261 ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg
    13321 ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc
    13381 cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg
    13441 cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga
    13501 atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc
    13561 ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat
    13621 gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat
    13681 ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg
    13741 attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga
    13801 tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac
    13861 acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa
    13921 agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt
    13981 tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga
    14041 aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag
    14101 ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga
    14161 cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat
    14221 gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg
    14281 gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag
    14341 aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa
    14401 ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta
    14461 atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct
    14521 ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa
    14581 agtatg
//
LOCUS       DASJRQ010000068        14390 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJRQ010000068 DASJRQ010000000
VERSION     DASJRQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.068"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..932)
                     /locus_tag="U4A80_001933"
     CDS             complement(36..932)
                     /locus_tag="U4A80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1975551.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(925..1605)
                     /locus_tag="U4A80_001934"
     CDS             complement(925..1605)
                     /locus_tag="U4A80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1975552.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1606..2388)
                     /locus_tag="U4A80_001935"
     CDS             complement(1606..2388)
                     /locus_tag="U4A80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975553.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2381..3298)
                     /locus_tag="U4A80_001936"
     CDS             complement(2381..3298)
                     /locus_tag="U4A80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1975554.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3462..3785)
                     /locus_tag="U4A80_001937"
     CDS             complement(3462..3785)
                     /locus_tag="U4A80_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975555.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4573..7800
                     /gene="fss3"
                     /locus_tag="U4A80_001938"
     CDS             4573..7800
                     /gene="fss3"
                     /locus_tag="U4A80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1975556.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7913..8227
                     /locus_tag="U4A80_001939"
     CDS             7913..8227
                     /locus_tag="U4A80_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1975557.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8240..8614
                     /locus_tag="U4A80_001940"
     CDS             8240..8614
                     /locus_tag="U4A80_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1975558.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8624..8959
                     /locus_tag="U4A80_001941"
     CDS             8624..8959
                     /locus_tag="U4A80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1975559.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9042..10382
                     /locus_tag="U4A80_001942"
     CDS             9042..10382
                     /locus_tag="U4A80_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1975560.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10460..11134
                     /locus_tag="U4A80_001943"
     CDS             10460..11134
                     /locus_tag="U4A80_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1975561.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11127..11291
                     /locus_tag="U4A80_001944"
     CDS             11127..11291
                     /locus_tag="U4A80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975562.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11367..12509
                     /gene="dcm"
                     /locus_tag="U4A80_001945"
                     /pseudo
     CDS             11367..12509
                     /gene="dcm"
                     /locus_tag="U4A80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12499..12789
                     /locus_tag="U4A80_001946"
     CDS             12499..12789
                     /locus_tag="U4A80_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975563.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13048..14232
                     /locus_tag="U4A80_001947"
     CDS             13048..14232
                     /locus_tag="U4A80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1975564.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14229..14366
                     /locus_tag="U4A80_001948"
     CDS             14229..14366
                     /locus_tag="U4A80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1975565.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4598 a         2634 c         3019 g         4139 t
ORIGIN      
        1 acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag
       61 atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat
      121 ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg
      181 gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg
      241 gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct
      301 gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct
      361 gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa
      421 aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg
      481 agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata
      541 ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta
      601 tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga
      661 tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt
      721 cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc
      781 ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg
      841 attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag
      901 ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg
      961 aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac
     1021 tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga
     1081 aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa
     1141 atggacagcc acaccattca catttacatc cctcgactct ttgtccagta agatttcctt
     1201 gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat
     1261 gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc
     1321 tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg
     1381 aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat
     1441 gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc
     1501 tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac
     1561 agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga
     1621 tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta gactgaccag
     1681 aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca
     1741 cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg
     1801 aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct
     1861 agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac
     1921 aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc
     1981 gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt
     2041 gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga
     2101 taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc
     2161 tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc
     2221 gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca
     2281 ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt
     2341 acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc
     2401 cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag
     2461 gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa
     2521 gctggataac acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg
     2581 ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca
     2641 attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg
     2701 cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt
     2761 ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg
     2821 tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac
     2881 gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca
     2941 tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac
     3001 gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca
     3061 tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa
     3121 tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca ttttccccaa
     3181 tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag
     3241 caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac
     3301 ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag
     3361 aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata
     3421 cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc
     3481 ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga
     3541 tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc
     3601 agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca
     3661 aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt
     3721 cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa
     3781 aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat
     3841 tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac
     3901 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa
     3961 tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg
     4021 gataaataat attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga
     4081 tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc
     4141 aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc
     4201 ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac
     4261 tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
     4321 gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt tgattaaatt
     4381 tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc agtatatttc gtattttaga
     4441 ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa aaattttaaa
     4501 aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct taatttgaaa
     4561 ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac tattttgagt
     4621 ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc tgatgaaatt
     4681 acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc tgttgaagga
     4741 acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc agtgtacaac
     4801 ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa tgagttcact
     4861 cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt agtttccgtt
     4921 ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa gatgatctgg
     4981 caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag tgccgtaaat
     5041 atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa gaaaccaacc
     5101 ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac ggatacgaat
     5161 aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat tgattatcgt
     5221 gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag tggtgtgctg
     5281 acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc tggccaacag
     5341 actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat tgatgtggaa
     5401 acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt tgtaccagga
     5461 acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac tactgacaaa
     5521 gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat taccgaaaaa
     5581 tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat taaagcaggc
     5641 gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat tctggataaa
     5701 agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc tggtaatacg
     5761 tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac cacggatgaa
     5821 aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg aacctattat
     5881 gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt aaaagtcgaa
     5941 ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg gcaaaatcaa
     6001 gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga taaagcacaa
     6061 ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga tggaaaagct
     6121 gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg ttctgatgaa
     6181 acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt agctatcaac
     6241 gagtattatt ggcaagaaac aaaagcgccc gaaggatata gccttgatga aacaaagtat
     6301 cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac ccgaaatgtt
     6361 acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc tggatcagct
     6421 gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc attggaaggg
     6481 accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga gcaattaggg
     6541 tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta
     6601 gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac ttttaaggaa
     6661 aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca agaccaaaaa
     6721 caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc attttctgtt
     6781 agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt aacttctctt
     6841 gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga gctagttgat
     6901 aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt
     6961 gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtgatt
     7021 gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa aattcagcat
     7081 aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta tgtgtatgaa
     7141 aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag cttgaataac
     7201 caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac aaaggcccac
     7261 ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt tgatgatgtg
     7321 tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat tctgtatgct
     7381 ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga ttatgaagta
     7441 aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg aaaatatcca
     7501 gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa tatcaatgga
     7561 aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga agttccaacc
     7621 atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc tcaagaatct
     7681 agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt tttactgttc
     7741 attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg ccggaactaa
     7801 ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc tcctaaaaaa
     7861 gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga agatggaatt
     7921 gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg ccggtgaagg
     7981 agaaattata acagaaggat atggacggaa tactacggtg attggtcgca gttatcattt
     8041 gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg aagccggtga
     8101 aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg ttgccaaagg
     8161 ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg atttagtgaa
     8221 agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt caggtttaac
     8281 atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa atgaggatgg
     8341 aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg cacaagggcg
     8401 aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt atgatgcaga
     8461 ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc gagaaggaac
     8521 taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc cgaatcaagg
     8581 aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa ttaatgttga
     8641 acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg aacaactcgt
     8701 taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt tacttggaga
     8761 aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag aagagtcttt
     8821 ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca ttttggaaga
     8881 aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg ttgaaacgaa
     8941 acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt gcctaatgaa
     9001 atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa ggacatcgca
     9061 tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta ctcgctctgt
     9121 tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat atttctaagc
     9181 tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt ctgcttggaa
     9241 gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt gatcattggc
     9301 gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat tggtatgaag
     9361 tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga accagagaga
     9421 ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg tctattcgcg
     9481 ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag aagttagaaa
     9541 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gagtatactt
     9601 tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca gaaaatggga
     9661 ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat atgttaatcg
     9721 ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa gcactattga
     9781 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct gatttaggta
     9841 cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg gaagatttct
     9901 atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac aaaacaggag
     9961 agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa tacgtggctt
    10021 atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc aaacaaatcg
    10081 tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg ccggatgcga
    10141 aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg ggtcgtatga
    10201 gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca
    10261 tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac
    10321 ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt
    10381 gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt atatagtagg
    10441 aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg caaaagcaaa
    10501 taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg gacgagacgc
    10561 agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg gattagctga
    10621 gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt ttcctgatta
    10681 taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag agcaggcatt
    10741 tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg caatattaag
    10801 tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga ttttctatgt
    10861 ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg cagagggacg
    10921 tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga acattgcaaa
    10981 agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt atacgaataa
    11041 tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac tagggtatag
    11101 taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg aaaattgtaa
    11161 agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa gagttcaaaa
    11221 cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta gttggattaa
    11281 ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt tcttggcttt
    11341 tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc ggtattggtg
    11401 gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt gaaattgata
    11461 aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg gaaatgcatg
    11521 acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac atcctttgcg
    11581 gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt tccgatactc
    11641 gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct aagcttttat
    11701 tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc gagacgatcc
    11761 tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac tcaaaggcct
    11821 acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac gcatgtaccg
    11881 aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc agaaatttgg
    11941 taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca gatggcgtag
    12001 cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg gatggaatct
    12061 ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat gaagttactg
    12121 gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt cgaaaaaatg
    12181 aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt cctaattcca
    12241 caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca ggagaccaac
    12301 aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag tgttggcgac
    12361 ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca gatagtcagt
    12421 tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt gccagaagat
    12481 taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta ttgggtgatt
    12541 ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa atttgatatt
    12601 cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga gacggatgct
    12661 cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca agcaaaagaa
    12721 ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa tggtattaca
    12781 gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag gattcgcgaa
    12841 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac catgatgtct
    12901 cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca accattaaga
    12961 atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat ttgaaaaagt
    13021 taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta tcggctatta
    13081 aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc ttgtggactt
    13141 agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat aaaaaccatg
    13201 aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac gttactgttt
    13261 gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca agaaattctc
    13321 catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta tcaagaacag
    13381 tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg tgtgctttta
    13441 gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca aaagcgtagc
    13501 tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg cttggacttg
    13561 gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa atgtcagaag
    13621 gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga acttttaaag
    13681 gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa gagtgaggtt
    13741 tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat tccattagac
    13801 caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc ttttcatgcg
    13861 attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat tatcaatcgt
    13921 tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc cttgaatgaa
    13981 caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc cgagccaaaa
    14041 ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc tacgtggaaa
    14101 atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat ggtacagaat
    14161 gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc aattgaaaat
    14221 ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt ttggtatttg
    14281 gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca gcagcggatc
    14341 gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt
//
LOCUS       DASJRQ010000069        14255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJRQ010000069 DASJRQ010000000
VERSION     DASJRQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.069"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="U4A80_001949"
     CDS             476..1339
                     /locus_tag="U4A80_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1975566.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U4A80_001950"
     CDS             complement(1468..3090)
                     /locus_tag="U4A80_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1975567.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U4A80_001951"
     CDS             3339..3860
                     /locus_tag="U4A80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1975568.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4A80_001952"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4A80_001952"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1975569.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U4A80_001953"
     CDS             5941..6759
                     /locus_tag="U4A80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1975570.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U4A80_001954"
     CDS             complement(6862..7059)
                     /locus_tag="U4A80_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975571.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U4A80_001955"
     CDS             7228..7806
                     /locus_tag="U4A80_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1975572.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U4A80_001956"
     CDS             7901..8038
                     /locus_tag="U4A80_001956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975573.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="U4A80_001957"
     CDS             complement(8851..9354)
                     /locus_tag="U4A80_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1975574.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="U4A80_001958"
     CDS             9713..10738
                     /locus_tag="U4A80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1975575.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="U4A80_001959"
     CDS             11061..11939
                     /locus_tag="U4A80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1975576.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="U4A80_001960"
     CDS             12415..12531
                     /locus_tag="U4A80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1975577.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="U4A80_001961"
     CDS             13459..13797
                     /locus_tag="U4A80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975578.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="U4A80_001962"
     CDS             13891..14121
                     /locus_tag="U4A80_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975579.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4524 a         2378 c         2725 g         4628 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttag
//
LOCUS       DASJRQ010000070        14228 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJRQ010000070 DASJRQ010000000
VERSION     DASJRQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.070"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..3708)
                     /gene="rpoC"
                     /locus_tag="U4A80_001963"
     CDS             complement(34..3708)
                     /gene="rpoC"
                     /locus_tag="U4A80_001963"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1975580.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3766..7383)
                     /gene="rpoB"
                     /locus_tag="U4A80_001964"
     CDS             complement(3766..7383)
                     /gene="rpoB"
                     /locus_tag="U4A80_001964"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1975581.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8111..9079
                     /locus_tag="U4A80_001965"
     CDS             8111..9079
                     /locus_tag="U4A80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1975582.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9097..10002
                     /locus_tag="U4A80_001966"
     CDS             9097..10002
                     /locus_tag="U4A80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975583.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9999..10805
                     /locus_tag="U4A80_001967"
     CDS             9999..10805
                     /locus_tag="U4A80_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1975584.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10941..11930
                     /locus_tag="U4A80_001968"
     CDS             10941..11930
                     /locus_tag="U4A80_001968"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1975585.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12247..12771)
                     /locus_tag="U4A80_001969"
     CDS             complement(12247..12771)
                     /locus_tag="U4A80_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1975586.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13058..>14228
                     /gene="scm"
                     /locus_tag="U4A80_001970"
     CDS             13058..>14228
                     /gene="scm"
                     /locus_tag="U4A80_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1975587.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT         4032 a         3076 c         2807 g         4313 t
ORIGIN      
        1 caataatttg tcacaacctc ttttttaaaa tatctatttt gtttgattgt tcattgcgtc
       61 ttctgcagcc atttgcgctt caatatctga aatactgtag acattttcgc ttgctacacc
      121 gacttctttt ggttccatgt cacgataacg ttgcatacca gtaccagctg ggatgatctt
      181 acctataata acattttctt tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc
      241 atccgtcaag acacgagttg tttcttggaa tgatgcagca gataagaaac tatttgtttc
      301 taatgatgct tttgtgatac ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga
      361 gactagcgta ttgtagtttt gatctttaaa gtctgcgatg tctaataatg tacctggtaa
      421 gatatctgta tcacctggat ccattacacg aactttacgc agcatttgac gaaccattac
      481 ttcgatatgc ttgtcgccga tttctacccc ttgcatacgg taaacacgtt gtacttcacg
      541 aagcaagtag ttttcaacag ataatacatc gcgaacttgc aacaattgtt ttggatcgat
      601 tgatccttct gtcaatggtg caccacggtg gatcatatcg ccttctgcta ctttcatacg
      661 tgctgtataa ggaacagtat acgtacgtgt atctgttttt cctttgattg ttacttcttt
      721 ggtacgtgtt gcaggatctt ctgagatatc gatgacatcc cctgttactt ctgtgatcac
      781 tgcttgccct ttcggattac gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt
      841 gatatcgtct ccggcaaccc cacctgtatg gaatgtacgc atcgtcaact gagtaccagg
      901 ttcaccgatt gattgagcgg cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc
      961 agttgcaagg ttgcgtccgt aacaatgttt gcagacacca tgttttgtat tacatgtgaa
     1021 gacagaacgg attgtcactg tttcgacacc tgcatcaacg atttgttttg cgatgtcttc
     1081 ggaaatcaat tggtttcctg caatgatcaa ttcatttgtt tcaggatgac gtacttcttt
     1141 acgggtgtaa cgtcccacta aacgatcttc taatgattcg atgatttcat ttccctcgcg
     1201 gatggcttgg atttcaagac ctcggtcagt accgcaatct tcttcacgga tgattacatc
     1261 ttgggccacg tcaaccagac gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt
     1321 atcggtcatc cctttacgag ccccgtgagt tgagataaac atttccaaga cggatagtcc
     1381 ttcacggaag ttcgaaatga ttggcaattc catgattcga ccattaggag cggccatcaa
     1441 accacgcatt ccggcaagct gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga
     1501 catcatgaag attggatttt tcgcttccaa accttccatc agtttttgtt ggatgctgtc
     1561 ttttgcagca ttccaaacag caatgacacg ttcataacgt tcgtcatctg tgatcagacc
     1621 acgacggaat tgttttgtaa tagtttcaac ttgtttatgg gcattttcaa tgatttcttg
     1681 tttttcattc agtacactga tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg
     1741 tttgtaccct aagtctttca tacggtcaag catcttagaa gtttctgtta ctttgaatcg
     1801 tttgaagact tctgcgatga tgtttcctaa gtttttcttc ttgaacggta atactaattc
     1861 ttgctctttg atatgtgcag gaatatcagt accagcttca acaaagtatt tgtccggtgt
     1921 ttggacggtc aagttgaagt ctgttggttc attcagataa gggaattcag gaggcatgat
     1981 ctcgttgaag atgatcttac caactgttgt gatcatcgta cgttcttttt gccattcagt
     2041 aaatggtttt tcacctaatg agttagggtt cacaccgata cgtgaatgta aatgaacata
     2101 tccgttgcgc catgcgataa cggcttcgtt ttgatctctg aagaccattc cttcgccttc
     2161 gcgtccttct tcttccattg tcaagtagta gttacctaga accatatctt gggaaggagt
     2221 aacaactggt ttaccatctt tagggttcaa gatgttttga gcagctaaca tcaacatacg
     2281 tgcttctgct tgtgcttctt cattcaatgg aacgtggaca gccatttggt ctccatcgaa
     2341 atcggcatta taagcttcac atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa
     2401 aactggttcg aatgcttgga taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac
     2461 tgggtgttcc ttgattacgt cttctaaaac atcccatact tcgtcttctt gacgttcgat
     2521 tttacgtttc gcatttttga tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac
     2581 gaatggtttg aataattcaa tggccatttc ttttggaagt ccacattggt acatcttcaa
     2641 gaaaggtcca acaacgataa cggaacgacc tgagtagtcg acacgtttac ctagcaagtt
     2701 ttgacggaaa cgaccttgtt tccctttcaa catatgagaa agagatttca atgggcggtt
     2761 acctggtcca gtaactggac gtccgcgacg accgttatcg atcaatgcat ctactgcttc
     2821 ttgcagcata cgtttttcat tttgaacgat gattccaggt gcattcaagt ctaacaaacg
     2881 tttcaaacgg ttattacggt tgattacacg acggtaaaga tcattcaagt cacttgttgc
     2941 aaaacgtcca ccttctaatt gaaccattgg gcgtaaatct ggtggaataa cagggataac
     3001 gtccataacc atccagcttg gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa
     3061 acggcggatt gcacgtgtac gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc
     3121 ggctgcttct gcatctagat caacattatc aagtaattgt ttaatcgctt ctgcacccat
     3181 agcagcttgg aattcttgtc catattgttc acgtttttcg cgatattcgc gttctgtcaa
     3241 caattgtttt ttctctaaat tcgtatttcc tggttcgatg actacatatg aagcaaaata
     3301 aatgatttct tccaaagcgc gtgggctcat gtctaataca agacccatac gtgaaggaat
     3361 ccctttaaag taccagatat gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc
     3421 acggcgaact ttcgaacgag taacttccac tccgcatcga tcacaaacga ttcctttata
     3481 acggatacgt ttgtattttc cgcaggcaca ttcccagtct tttgtaggac caaaaatccg
     3541 ctcacagaac aacccttcgc gttcaggttt taaggtacga tagttaatcg tctcaggttt
     3601 ttttacttca ccgtaagacc agcttctgat tttttcggga gaagccaaac ctatttgcat
     3661 actttcgaat ttatttacat cgatcaaaag gggttccctc catttcattt aatggcaaac
     3721 cgcaagacgg ctgatcttcg accgccttgc agctcataga ccggattagt ctaaattatc
     3781 ttctgcggtt tcaaagtttt ggatgacatc gtctttttca tcttccactt gttcagcagc
     3841 tttcttttct aattctttgg cagtttgctg ttcggcaaat tttgtcaaag catcaacagt
     3901 gatcaaatcg tcgtcttcat cgtccatgtc gcgaagttcg atctctgttt cttcgatatc
     3961 caagacacgc atgtccagac ctagtgattg caattctttc accaatacgc ggaatgattc
     4021 cggtacacct ggttttggaa ttggttcacc cttgacgatt gcttcgtatg ttttcacacg
     4081 tccgactacg tcatctgatt tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta
     4141 agcttccagt gcccatactt ccatttcacc aaaacgttgt ccaccaaatt gagctttacc
     4201 tcccaatggt tgttgtgtaa ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc
     4261 atcgaccatg tgagcaagtt tgatcatgta catcacaccg acagacacac gtccatcaaa
     4321 tggttcacct gtacgtccgt cgtacagaac agttttcgca tcgctggcca taccggcttc
     4381 acgaactgtt tcccatacgt cttcatcact tgcaccatca aatactggcg ttgctacgtg
     4441 gatacctaac tgacgtgcag ccatacccaa gtgtaattcc aatacttgcc cgatgttcat
     4501 acgtgaaggc acccctaaag gattcagcat gatatcgatt ggtgttccat caggtaggaa
     4561 tggcatatct tcttccggca taatacggga tacaacccct ttatttccgt gacgaccggc
     4621 cattttatcc ccttcatgga ttttacgttt ttgaacgata taaacacgaa ctaacatgtt
     4681 tacgcctggt gacaattcat ctccagcttc acgagtaaag attttcacat cgtgaacgat
     4741 accgccgccg ccatgtggta cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc
     4801 aaagatcgca tgcaatagac gttcttctgc agataattct gttacccctt ttggcgtgac
     4861 tttccctaca agcaaatcgc catcttggac ttctgcacca atacggatga tacccatttc
     4921 gtctaaatct tttaatgcgt cttccccgac attagggatt tcgcgtgtga tttcttcagg
     4981 tcctaatttt gtatcgcggg cttctgattc atattcttca atatggatag aagtgtatac
     5041 gtcatctttt accaaacgac ggctcatgat gatcgcatct tcatagttgt acccttccca
     5101 agtcatgaaa ccaactaaaa cgttttggcc tagagccatt tcgccttgtt ccatagatgg
     5161 tccgtctgcc agagtatccc ctttttcgac tttctcaccc aaatgaacga ttggacgttg
     5221 gttgtagctt gttccggagt ttgaacgacg gaattttgtt acatcgtatt tatctaatgc
     5281 accgttgtcg cggcgtacgc ggatttcaga agcatcaacg tattcaacga caccgtcatg
     5341 tttacataac aaagcagctc ctgagtcatg cgctgatttg tattccatac cagtaccaac
     5401 ccatggtgct tgaggattga tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa
     5461 ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac
     5521 ttgtttaggt gatacgtcca tgtagtctac tttatcaata gatacttcta agttttcact
     5581 ttgagcacga gccataacaa cgtcttgagc aaatgttcca tcttcattca atgggctgtt
     5641 tgcttgggct acgatgtaat ggtcttcgat atcagctgtc aagtagtcga tttggtcagt
     5701 aacacgacct gtttcacggt ctacacgacg ataaggtgtt tcgataaatc cgaatttatt
     5761 tactttcgca taactggaca aactattgat caacccgatg tttgggcctt caggagtttc
     5821 gatcggacac atacggccat agtgggagta gtgaacgtca cgaacttcat aaccggcgcg
     5881 gtcacgagtc aatccgccag gtcctaaggc tgaaagacga cgtttgtgtg tcaattcgcc
     5941 aagagggttt gtttgatcca tgaactgtga caactgagaa gaaccaaaga attctttgat
     6001 acttgccaca actggacgga tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg
     6061 gatcgacatt ctttcacgaa ccacacgttc catacgtgct agcccgatac ggaattggtt
     6121 ttgtaaaagt tcaccgactg aacggatacg acgatttcct aagtggtcga tatcatcgac
     6181 attgccgata ccttccatca agttcaagaa atagctcatt gaagcaatga tgtcggctgg
     6241 acgaaccgtt tttacagctg cttctggata gccgtttccg atcacgttta cttcacgttc
     6301 agggtctctt ggtgaaaata ctttgatgac ttggacagtc attggatctg tgaccacacc
     6361 gtcttctgat ggatagtaag tcactgaatt taggccatta tcaatgaaag gagctagtgt
     6421 ttccatgact tggtgagtca gaactgttcc tttttcaacg atgatctcgc ctgtttctgg
     6481 atcaactaat gtttcagcta atgtcagatt caatagacgt gttttcaaat ctaatttttt
     6541 gtttactttg taacgaccaa cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc
     6601 attcagcaag ttacgtgagc tgtctgctgt ttttggttcg cctggacgca aacgttcgta
     6661 aacgtctttc aaaccttctt ctgttcttga gtcgcttgcg tttttatgca agtctttttc
     6721 gactgtattg cgtaatgttt cgctgtcgcc gaagatttca aagatcgtat catcggaacc
     6781 aaatcctaac gcacgaacta atacggttaa aggaatctta cgtgtacggt cgatacgtac
     6841 ataagagatg tcttttgcat cagtttccat ttctagccaa gctccacggt tagggataac
     6901 agtagaaccg aatccttctt ttccgttttt gtcgactttt ccatggaaat agacacctgg
     6961 tgaacggact aactgagaaa cgattactcg ctctgccccg ttgatgatga atgttccttg
     7021 ttctgtcatt aatgggaaat caccgaagaa tacttcttgt gctttgattt cgccagtctc
     7081 gcggtttgtc aaacgcaatg ttacatgtaa tggtgcagag tagtttgcat catgcgcacg
     7141 agcttctgct actgtatact ttggttcttt caattcataa tctacaaatt ccaaagatag
     7201 attgccattg aagtcatcga ttggcaaaat gtcttcaaac atttctctaa gtccctcatc
     7261 taagaaccat tggtaagagt ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc
     7321 actgattctt gcgaaacttc tacgttcgcg atgttttccg tattttacta cgtgtccagc
     7381 caagctcttc acccctctaa aatgtcattc aaaaagacta cccgatcaat tgttacaata
     7441 atatttcaaa tattattttt aggtaacaac agacattttt tgcggctttt aggcaaaaaa
     7501 aaaccaaaaa tagaacattc tcttgaaacc ttacttcgtg agcttctact tttgtttcct
     7561 tatattaaag ccgaaacgga caatatttcg tttctgcctt ttcttcatca gatagttttt
     7621 gcaacttcat atactacact tattttctat aaaagtcaac acttcctggc gtttcttctg
     7681 ctttttcgca acattttctt aatgttttca attttatgtt ttcaattatc tgacaattct
     7741 taattgacag acctttttat ctcggttata ctttagaaaa ttcattttgt ggaggaactt
     7801 ctatgcgaac caacatcaac caaatcgcac caacacaact gaataacttt tactttagct
     7861 actttagata ggtttgtatt catcatgatg gatacaaacg ttaaacgaca gtttgtatct
     7921 atgatggcta aagaactttg tattgcagca aaaagccacg tagacaaaat gctaagtggc
     7981 tgagcagtga aaatacaaag taaaagtatc aagcattcta ctttttcaat ttattttccg
     8041 acttttcaat ccatttggac attttgtctg aatggatttt ttgtttccca aaattaagga
     8101 ggaaaaagaa atgaaaaaaa atataatcat cggattaggc gctgccctgc tactagcttt
     8161 aggaggttgc gcacaaaaag acaattccgc agaggcatca gatggacaaa aaacaattgg
     8221 cgtattgcaa cttgtacaac ataactcgtt agatgctgcc tataaaggat tcaaagaagg
     8281 attagcagaa gaagggtata aagaaggcga taatctaaca atcgattatc aaaatgccca
     8341 aaacaatcaa gacaacctga aaagtatgag tgagaaactc gtcaaagaag atcctgattt
     8401 gctacttgga atcgccactc cagcagcaca atctttatta aatgaaacaa ccactatccc
     8461 gatgactgtc acagcggtca cagacttgga acaagcccat ttagtcacat ccaatgaaaa
     8521 acctggcggc aatgtgactg gcacgacaga tatggtgcca atcgaaaaac aaatcggctt
     8581 actgttaaat atcgtgccag acgccaaaac aatcggcatc atgtataaca acggtgaagc
     8641 aaactctaag atccaagctg atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt
     8701 caaagtactg acagcaaatt ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa
     8761 agatgtagat ggaatctata ttcctactga taacacgttc gcaagtgcgg caagtgttgt
     8821 cggtgaagtc gcaaaagaaa caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa
     8881 agacggcggt ctagcaacag taggaatcga ctacgaaagc ttgggtaaac aaacagggat
     8941 catggcagca aaaatcttag acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa
     9001 tgatttggaa ctgtatgtca ataaagacat ggccaaagca ttaggcatca atccagacag
     9061 catcaaagaa gctgaataaa acacaggagg attctcatga caaatttgct ctctcttttc
     9121 ttatcatctg catcgcaagg ggtcttatgg gccctacttg cgattggtgt atacgtcact
     9181 ttccgtatct tggacattgc cgacttgact gctgaaggaa gctttccttt aggtgcaggg
     9241 atcgcggcag taagtattac aaatggctat catccattga cagcttgtct tctaggcttt
     9301 ttagggggtg cggcagcagg gcttgtttcc ggactgcttc acacaaaatt gaaaattccc
     9361 gcccttcttg cagggatcat caccatgact ggtttatatt ctgtcacatc ccgtgtgatg
     9421 ggtgcgccga acatctcttt attgggtcag ccaacgatct ttcattgggt cgagtcattc
     9481 ggcataacaa aagacaacgc tgtactgatc gtcggtctgc tttttgcttt gatcgttgta
     9541 tgtttacttg ttttgttctt acgtacagaa acaggacttg ctattcgtgc aactggcgat
     9601 aatctggcaa tgagcgaagc aaacgggatc aacacagata atatgaagat catcggttat
     9661 atgatttcta atggatgcat tgctctttct ggctccttgc ttgcgcaaaa caacggcttt
     9721 gctgatttaa attctggtat tggaacgatc gtaattggac ttgcttctat tatcatcgca
     9781 gaagttttgt tacgtaacca gccactattt ctccgtctct tgactgtcgt tttaggtgcg
     9841 gtcatttatc gttttatttt agcgttagtc ttcgaactga atgttgaacc aagtgattcg
     9901 aaacttgctt ctgctttcgt gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa
     9961 ttccatttac ctcaaaaaaa gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg
    10021 aaaatctcca tcataccttt gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca
    10081 ttaatctttc cttagatccc ggcgacttcg tgacgatcat cggtggaaat ggggctggta
    10141 aatctacttt gctgaacagc atcgccggta cattttctgt cgatcagggt caaattttat
    10201 tgaatggaaa aaatatcacg aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg
    10261 tattccaaga tcctaaacta gggacagccg ttcgtttaac ggtagaagaa aatcttgctc
    10321 ttgctatgaa acgaggaaaa aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa
    10381 catttttcaa agaccagctg gttcggctaa atttggggtt ggaaaatcgt ttatcctcag
    10441 aaatcggttt gctgtctgga ggacaacgtc aagctattac gttattgatg gccacattac
    10501 agcgtccaga actgatctta ttagatgaac acactgcagc gcttgaccct gctacttcca
    10561 aaacggtgat ggaattgaca gaaaagctga tcactgagca gaatctcact gcttttatgg
    10621 tcactcatga catggaagac gcgatccgct atgggagtcg actgatcatg ctgcatcaag
    10681 gaaggatcgt cgtagatatc cagcaagagg aaaaacagca gctcacagtg ccccaattat
    10741 tagcgttgtt caaacaaaac agcggcacgg cattaaaaga cgatcaagta cttttatctt
    10801 cataatcaga aagaggatgg gacaaaaaat gaacgcccac cctcttcttt tttctctttc
    10861 cttctttcgt ttttgcttta tgtatcaacc gttgtttctt cgaaaactat tttgttctct
    10921 ccttcttcct gatagaatgg atgggaaagg agcttgatgc catgacaacg aaagacaagg
    10981 tacttcaatt attgaaaaag acaccagatt ttctttccgg agaaaagttg gcacagcaac
    11041 tggaagtttc ccggacaagt atttggaaag ccatcaaaga attggaaaag gcaggctatc
    11101 gttttgagca cgggcctaca gggtatcgtt acctgccctc tgacgtacta gatgcaaaag
    11161 aaatccagca agagctacgg tcgtttattc ctgatttgaa agtacaaacg atgttgactt
    11221 ctgaatctac gatgaaagat gtcaaactgg cagcagctca aggagattct gtttcgaagt
    11281 tatttgttgc tgatacacaa gaacaagctc gtggacgttt tggacggcct tttttcgctg
    11341 aacctggacg aggaatctac atgagtctct tgctccatcc agacaaacgg tttgatgaat
    11401 tgcctcaata tacgatcatc gcagccagtg cctgtgtgaa agccattgaa tctgtaaccg
    11461 gtcgttctgc ttcgatcaaa tgggtcaatg atatctacat ggatggaaaa aaagtctgtg
    11521 ggattttatc tgaagcagtc agtgatatgg aaagcggcag gatcagtcat atcatcattg
    11581 ggatcgggct taatttttct gttcctcaaa atcattttcc agctgatctc caagaaaaag
    11641 ccatctctgt ctttcccgac ggacaagcta gtgtcacaag gaatcagcta atcagcaaaa
    11701 tctggcaaga attgttcact tatctttctc acccagaaaa agcgttggac ttctataaag
    11761 aaaaatcatt cgtattggga aaaaccgtgt cgttcaatca aaaaggaacg acttatgtcg
    11821 gtcttgcaaa agaaatcggt gatcatggcg aactgatcgt ccaaacaaat gaacagaaga
    11881 tgagtttgtc ttctggcgaa atcagtctca catctatccg tacgcaataa ttatcagatt
    11941 tcacaaaagg acacctgatc tcatacagtg tccttttttc tgaattctct tcaaaagact
    12001 ctccttattc tatcagctga cagtttctct atcctctaga aaaaggtatt ctaaaaatat
    12061 ctataaagca tggtacactt tctttaaatg aatcaatgca aaaaacaaga gagtcatcag
    12121 tttacacgat caaaagagat agcaggccgc ttcctttttc aatcttatag atagaccatg
    12181 ggtaacgata aaaacaaaaa agaacttgcg acttattttg ccgtaagttc ttttttagct
    12241 agtgccttaa tctcttaatc ccaacaaacg tctcgcatag atcacctgac cagagtgcat
    12301 agcagcatca tcaataatag aaacgagacg cattcctcgt gtgactggtg gtgtccagtt
    12361 ctcgtcaatt acttcatcta gctcggaagg ttccagcctt cttaagtaag aaatagcaaa
    12421 atcagcagct cctcgcaagt atcctagcag atcttctatt tgattagctt ggatctgctt
    12481 tgcttgttcc agactatgct tccaatcttg ttcgttatct gctatcgtca tcggaaaccg
    12541 gctttcccag tcttgactat gccaaattgg agaagttcct gctaaatcag caatctggaa
    12601 atctaattct cgagcggtat gccaagccag ccaagtcatt gatttgatct gcggggctct
    12661 atcttccact ggaaaacgat tggcctcctc aatagagacg ccttcaaaca aacgttcaaa
    12721 ccgatcgtat gcccgatcca cactctcgca tagcatatct aaataatcca tttattttcc
    12781 tcctttttat ttatcctact tactttttcc taattctagg atacctactt atatgttact
    12841 acacaccctt attttttaca ctttaacagt caccatcatc aatagtttgg agattgaaag
    12901 aaaaagatag tgtcgaacta tcaattgaac aagaagcaac aatatgaacg tttcattaat
    12961 attctttaaa taaacaatga tttttcaaac gaacagttta agactaggag aaaaagagta
    13021 taataggaaa atgtttgaaa ggaaaggaag gggaaaaatg aaaaaaaggg caagtttact
    13081 agtcctagtt gctttgttgt tcaatatctt ttcgtatggt gtggccgttt cagcggaaac
    13141 atacattcct aaagaccctg ggaatattta taatgatcta actggtaact atggcttgtt
    13201 aggaattgct agtcaattcc acgtatttgc taaaaataag acaactgtga atgcacatac
    13261 agacggaaat atagcaacac atgaacttga tgcaaaaaat aattttggta cagagcttca
    13321 tgaaggttta ttgaaccaag aaataaatta tatacaaaaa actgattctt tcgctaattc
    13381 ttcaggcata cctacaggta gcacaagaat gaataaattt gtcgttggca gtaacgtgga
    13441 tgtagcactt caagatggtc atcctacagt caatggatcc aggcttgatc acttaactcc
    13501 agatgatttt tataaagatc gtgctgggca tgaatatatt gatttcgagc aggaatttgc
    13561 taaattgaat gtagcagcaa atgatcttgc aacgatcact ccagctaaaa catatactgc
    13621 tgcagatttt ccagacatga acaatcggac aatcgattta actgatttat cagataccgg
    13681 gtttttattg gtaaatatcg atgcagaagt gttaacgatg aatacgccac ttcaaatcat
    13741 taatccaaat gatcaagtag ttgtatttaa tgtcatcaac agttcttctg cattgaacgt
    13801 acagtcacca atcaaataca atgatcgttc aaatcatgaa acagaagatt tttcggatgc
    13861 gaatatctct tggaattttg gaaatgagat gactgatttg acaatcagtg ctccttttca
    13921 aggaacaatc cttgcgccta atgctactat ccgcattact caaaaccaag acggaacgat
    13981 tataggaaca aatgtgattt tagatgcagc taccaatcgt tgggatccta acgaaatatt
    14041 tatcagcgac aatggtacag atacgacaga cacaagcgac agtacagata catctaccac
    14101 aagtgatagc tcagattcat caacttcaag cgacagtaca gatactacct ctaccacaag
    14161 tgatagcacg gatacatcag cttcaagcga cagtacagat actacctcta ccacaagtga
    14221 tagcacgg
//
LOCUS       DASJRQ010000071        13959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJRQ010000071 DASJRQ010000000
VERSION     DASJRQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.071"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..135
                     /locus_tag="U4A80_001971"
     CDS             <1..135
                     /locus_tag="U4A80_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1975588.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="U4A80_001972"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="U4A80_001972"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1975589.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="U4A80_001973"
     CDS             complement(2412..2663)
                     /locus_tag="U4A80_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1975590.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="U4A80_001974"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="U4A80_001974"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1975591.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="U4A80_001975"
     CDS             3613..3831
                     /locus_tag="U4A80_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975592.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="U4A80_001976"
     CDS             complement(4120..4632)
                     /locus_tag="U4A80_001976"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1975593.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="U4A80_001977"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="U4A80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1975594.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="U4A80_001978"
     CDS             complement(7085..7528)
                     /locus_tag="U4A80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1975595.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="U4A80_001979"
     CDS             complement(7529..8317)
                     /locus_tag="U4A80_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1975596.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="U4A80_001980"
     CDS             complement(8314..9255)
                     /locus_tag="U4A80_001980"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1975597.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="U4A80_001981"
     CDS             complement(9432..10019)
                     /locus_tag="U4A80_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1975598.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="U4A80_001982"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="U4A80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1975599.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="U4A80_001983"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="U4A80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1975600.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="U4A80_001984"
     CDS             complement(12936..13871)
                     /locus_tag="U4A80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975601.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DASJRQ010000072        13640 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJRQ010000072 DASJRQ010000000
VERSION     DASJRQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.072"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            43..1470
                     /locus_tag="U4A80_001985"
     CDS             43..1470
                     /locus_tag="U4A80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975602.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1562..1927)
                     /locus_tag="U4A80_001986"
     CDS             complement(1562..1927)
                     /locus_tag="U4A80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1975603.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1924..2289)
                     /locus_tag="U4A80_001987"
     CDS             complement(1924..2289)
                     /locus_tag="U4A80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1975604.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2481..2729)
                     /locus_tag="U4A80_001988"
     CDS             complement(2481..2729)
                     /locus_tag="U4A80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975605.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2728..2856
                     /locus_tag="U4A80_001989"
     CDS             2728..2856
                     /locus_tag="U4A80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1975606.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2913..4607
                     /locus_tag="U4A80_001990"
     CDS             2913..4607
                     /locus_tag="U4A80_001990"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1975607.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4611..4730
                     /locus_tag="U4A80_001991"
                     /pseudo
     CDS             4611..4730
                     /locus_tag="U4A80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4707..5003
                     /locus_tag="U4A80_001992"
     CDS             4707..5003
                     /locus_tag="U4A80_001992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975608.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5381..6514
                     /locus_tag="U4A80_001993"
     CDS             5381..6514
                     /locus_tag="U4A80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1975609.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6545..6865
                     /locus_tag="U4A80_001994"
     CDS             6545..6865
                     /locus_tag="U4A80_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1975610.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6894..7358)
                     /locus_tag="U4A80_001995"
     CDS             complement(6894..7358)
                     /locus_tag="U4A80_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975611.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7489..8742)
                     /locus_tag="U4A80_001996"
     CDS             complement(7489..8742)
                     /locus_tag="U4A80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975612.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8771..9088)
                     /locus_tag="U4A80_001997"
     CDS             complement(8771..9088)
                     /locus_tag="U4A80_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1975613.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9114..9434)
                     /locus_tag="U4A80_001998"
     CDS             complement(9114..9434)
                     /locus_tag="U4A80_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975614.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9898..11313)
                     /locus_tag="U4A80_001999"
     CDS             complement(9898..11313)
                     /locus_tag="U4A80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1975615.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11330..12646)
                     /locus_tag="U4A80_002000"
     CDS             complement(11330..12646)
                     /locus_tag="U4A80_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975616.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12796..13494)
                     /locus_tag="U4A80_002001"
     CDS             complement(12796..13494)
                     /locus_tag="U4A80_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1975617.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4324 a         2776 c         2351 g         4189 t
ORIGIN      
        1 tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt
       61 gagaaaaata ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc
      121 aatctagaaa agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc
      181 aataacataa aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt
      241 tacactttat atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa
      301 aattcgttgt ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt
      361 caattaactg aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta
      421 ttagcttatt ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa
      481 atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg
      541 gagctacctg aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat
      601 agtggcagat tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt
      661 ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa
      721 aaacgtccta tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat
      781 tataaaaaag aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc
      841 cattcttatc agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat
      901 actcccgaaa taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc
      961 ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat
     1021 caattattca tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa
     1081 gaagtccaga caattttttc cagctggtcc agccagttac cttatgatct acggttgaat
     1141 cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa
     1201 catctgacta tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc
     1261 ttggaagctg taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct
     1321 gatataaaga agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat
     1381 acaccagatg cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga
     1441 gctatcattt cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct
     1501 cttatttgtt gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac
     1561 ctcacttatt ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg
     1621 ttcggataac taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg
     1681 attcgatgat cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca
     1741 tctttttaat agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata
     1801 aatatttcaa aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa
     1861 agatatcaag tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg
     1921 tcgtcatcct acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa
     1981 ataagaaatg actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt
     2041 cggcttgata actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat
     2101 ataacttgct agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt
     2161 cttgacatga gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat
     2221 accgatagaa caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga
     2281 gcgaaacata tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga
     2341 aagttacctt gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt
     2401 ctttttcact ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac
     2461 gtagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt
     2521 gctttttttg tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc
     2581 ttacctaccc tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt
     2641 cctttttctt ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata
     2701 gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt
     2761 ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat
     2821 gacagtcatt tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac
     2881 ggacatactt ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg
     2941 gtgccggtat tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag
     3001 gggatcagat caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg
     3061 acactgtagt ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata
     3121 ttgctcgagg cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa
     3181 gtcgaatacc ttctgttact gagccaggga tgactgctga agaagatacg agagattttg
     3241 atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg
     3301 atggcggaaa actgggattc aataataaag accgtattct tctgactaaa ctgattgcta
     3361 tgcctgattc agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg
     3421 atccacactt ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa
     3481 cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc
     3541 aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc
     3601 ttcctttgat cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca
     3661 ctgactggga attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa
     3721 ttaccaatac actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta
     3781 ttttcactaa tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg
     3841 tagaaaatat ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct
     3901 acaacttggg aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct
     3961 ttaccctgac gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag
     4021 aaccaggaaa tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa
     4081 aagatgtcaa tatgtctatc gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa
     4141 atgaaacagt tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata
     4201 aacagtatat taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa
     4261 aagtagatcc tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa
     4321 taaataatat ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa
     4381 gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg
     4441 tcgagcagcc ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg
     4501 ctgctttcca ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca
     4561 aagatattcc tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg
     4621 aaaaagctgc cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt
     4681 tgatttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
     4741 gtgaaaaagc tgccctgcat caagtaataa gaacacccaa gctaaaactg cttcttatgt
     4801 ttttgacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttaccaagag
     4861 gttatgaaaa agctgccctg catcaagtaa taagagcagc caagctaaaa cagcttctta
     4921 tgtttttgac ttggttagac ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
     4981 gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa aattttgtga aaatggttcc
     5041 tcacgttttt gcataaattt gacttaatac cgagaaactg atcccttctg tcataacctc
     5101 tttttattta tgtctgtttc tttttgaaaa caactatttt ttcaacgttt actctcttgc
     5161 caatccaaca tcatctaacg gatacaacta tttcaatgat taccattaca ctaaaaaatc
     5221 ggaaacacac ctctccgaac acaaatacat aagactatag aatgtttcat ttaagaaaaa
     5281 aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat gaaattatct gtagtactaa
     5341 taatgcagac aattttatga atctgaaagg agcatctcca atgaaagcag tcacttggca
     5401 aggaaaacag aaaatggaaa tcagaaacgt agatgatcca aaaatattag aaccaacaga
     5461 tgcaatcatc cgtatcacag caacagccat ttgcggctcc gatctgcatc tttaccatca
     5521 tggtgaagca gttttggaaa aagggtatgt tgtaggacac gaaccaatgg gaatcgttga
     5581 agaagtggga tctcaagttc aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa
     5641 tattagttgt ggtcattgtc atttctgcct gaatcacatg gaaagccaat gtgatgaatc
     5701 aaatccagat cagttatatg gcgggctttt cggatttggc aagttgaacg gtaatcattg
     5761 gggtggtcaa gcggaatatc taagagttcc ttatgcagac tctacttcat ttgttgtacc
     5821 agataatgat cttcctgatg aaaaagtttt atttctatca gatattttgc ccacagctta
     5881 ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca gttgtgattc taggttctgg
     5941 tccagtcggt ctattcgctc aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc
     6001 agtagatcca gtacagcacc gtctagataa agcggctaat tacaatgggg ttgaaactta
     6061 tttcttagaa gacacggcac aagctggaac agaattgtat gaactggcta aaggtggcgc
     6121 agatgtcatt atcgattgtg taggaatgga cggattagag cctgtcaaag aaaaagcgaa
     6181 aaatttagta agcttacaaa gcggcacaat ctctccatta cagatggcat cgcaagctgt
     6241 caaaaaattt ggaacagtcc aagtaactgg tgtttatatg actccagctt cttcttaccc
     6301 gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat ggacaggcac caattatcca
     6361 tctaatgcca aaaatttacg caatgattgc agaaggacta ttcgatccta cacaaatcat
     6421 tactcattcg ctgccactat cagaagcagc acaagcatat gagatctttg acaaaaaaga
     6481 agataaaaat ataaaagttg ttttgaaacc ttgaattcag taaaaaatac aggtgatttt
     6541 ctttatgccc aaaatcaaac gaaataccca tgagatcgaa ataggaaaag aagaagatat
     6601 cgaaatacga ggacgtcgct tccgcttcta tttccaaaat cgttacacat tgatctcact
     6661 ggctgtcgat ttattaaccg ggattttcta tatttttgga agccttgctt ccttaactaa
     6721 aatccccgat cgatatggca cctattttta tttggctggt gctattttct tgacgattcg
     6781 tcctattctc agaatcgtaa ggaacatttt tatttacgac gaaaaaaaag caatgaagag
     6841 aacaagcaaa aaaaaagagg catgagaatt tttcaaatct catgcctctt ttttcaccct
     6901 atcgtatcac tttgatacag acgagcggtc gtcccacttt cttccttgac cattgtcaaa
     6961 aatgtccact cgtatcgttc ataaaacgtg gtatgatctg taattaagta aagtttttcg
     7021 tacccaagtt gatgcatttc ttttctgatt tccgctaata tccgcttggc tatcccctgc
     7081 ttacgctttt ccggttcaac aaacagggca caaagatttg gtgtcagatc agggcggtta
     7141 tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg tttctttttc atctaagacc
     7201 acataccatt gaggaatact ttccttatta gcgattccag tacgaatact ttcctcatac
     7261 acaacagcag gaactcccca ttttgatgaa aaccaattcg cgctttgttt tactaattct
     7321 gggtgttctc ttaatttgat ccagttatat gtaaccaatg tttaaacctc cgactaaaat
     7381 aaaacgttta caaaaattat acaagtcttt atgaaaatta gcaatgacaa caatagtgat
     7441 gtaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa
     7501 agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat
     7561 agaaagaacc aaattgacag cggctagtac accacctgca atactttttg ttgccaatac
     7621 accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc
     7681 tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat
     7741 gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa
     7801 gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac
     7861 taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct
     7921 gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgc ccagctgcga ttgcattaac
     7981 gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc
     8041 gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata
     8101 agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc
     8161 atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat
     8221 tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt
     8281 gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac
     8341 aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa
     8401 agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg
     8461 ataagcaagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc
     8521 agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc
     8581 gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta acggcatact
     8641 caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac
     8701 aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct
     8761 ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg
     8821 taaaagctat agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa
     8881 cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta
     8941 aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag
     9001 cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga
     9061 tggcttctag atttttttga tcttccattt attgtatcct ccgataattt ttattatttg
     9121 tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc
     9181 atattgatta catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc
     9241 ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta
     9301 tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc
     9361 ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt
     9421 gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta
     9481 gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca
     9541 agttgcatac acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc
     9601 ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca
     9661 aataaacggt attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata
     9721 agatgttgtt tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct
     9781 ctcaataaac ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca
     9841 acctctttta tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta
     9901 tttatagatt tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga
     9961 agcagatttt ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata
    10021 tcgattctta aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc
    10081 ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa
    10141 ttcgatacgg taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc
    10201 tcttcccata ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata
    10261 catggctata tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt
    10321 cttcccaggt aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt
    10381 ccattgataa cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc
    10441 tactgtatga tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg
    10501 tttttgacac aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa
    10561 tactcgatta taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga
    10621 tcgagcatag accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct
    10681 gcggtaaatc tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca
    10741 cttttttgtg ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg
    10801 aacgattggt tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc
    10861 aatttgagca tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt
    10921 ttccaaaaga aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc
    10981 ttcgatcaaa gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca
    11041 gttctcataa tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg
    11101 gatggatttc atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc
    11161 tgggccatac ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga
    11221 atcctgtcct tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc
    11281 aagaataaaa ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat
    11341 cattttccac acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa
    11401 tacaaacaag ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga
    11461 cgatcggcgg cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg
    11521 ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa
    11581 ttggattcag catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag
    11641 gagcaaggga taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg
    11701 caatcagtaa accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg
    11761 gcatagtaat aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa
    11821 ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa
    11881 tcacccaaaa gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga
    11941 caccttctaa tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa
    12001 gttctttgat gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata
    12061 aagcagaaaa actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt
    12121 tcttctgttt cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca
    12181 aaaataaccc tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct
    12241 gattttcatt cactaattca taaacagttg gattaacaga taaataagac atcactgcga
    12301 tgataccagg gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa
    12361 aaacagcata gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct
    12421 ttaattcttc taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga
    12481 ccattgctga aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa
    12541 atgcgttttt tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta
    12601 aaacattggc taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc
    12661 cttgacgtca tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat
    12721 ttactcctct tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat
    12781 acatcagatg aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg
    12841 atttattgcc atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat
    12901 ggcctgaacg atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata
    12961 atttttatta aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac
    13021 gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg
    13081 tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg
    13141 attcttcgtt gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag
    13201 ttcaaataag tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc
    13261 attggcaatc cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat
    13321 attcctggaa gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata
    13381 atcggacaat tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa
    13441 atatctaagg atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca
    13501 tacctccttt tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga
    13561 tgattttttg tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt
    13621 taccataaaa aagactagga
//
LOCUS       DASJRQ010000073        13572 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJRQ010000073 DASJRQ010000000
VERSION     DASJRQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.073"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..1040)
                     /locus_tag="U4A80_002002"
     CDS             complement(93..1040)
                     /locus_tag="U4A80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1975618.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1230..1499
                     /gene="rpsN"
                     /locus_tag="U4A80_002003"
     CDS             1230..1499
                     /gene="rpsN"
                     /locus_tag="U4A80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1975619.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1675..2037)
                     /locus_tag="U4A80_002004"
     CDS             complement(1675..2037)
                     /locus_tag="U4A80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1975620.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2048..3169)
                     /locus_tag="U4A80_002005"
     CDS             complement(2048..3169)
                     /locus_tag="U4A80_002005"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1975621.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3185..3535)
                     /locus_tag="U4A80_002006"
     CDS             complement(3185..3535)
                     /locus_tag="U4A80_002006"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1975622.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3665..5176)
                     /locus_tag="U4A80_002007"
     CDS             complement(3665..5176)
                     /locus_tag="U4A80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1975623.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5498..6850)
                     /locus_tag="U4A80_002008"
     CDS             complement(5498..6850)
                     /locus_tag="U4A80_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1975624.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7003..8079)
                     /locus_tag="U4A80_002009"
     CDS             complement(7003..8079)
                     /locus_tag="U4A80_002009"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1975625.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8275..10020
                     /locus_tag="U4A80_002010"
     CDS             8275..10020
                     /locus_tag="U4A80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1975626.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10300..10737)
                     /locus_tag="U4A80_002011"
     CDS             complement(10300..10737)
                     /locus_tag="U4A80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1975627.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10721..12094)
                     /locus_tag="U4A80_002012"
     CDS             complement(10721..12094)
                     /locus_tag="U4A80_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975628.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12416..12904)
                     /locus_tag="U4A80_002013"
     CDS             complement(12416..12904)
                     /locus_tag="U4A80_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975629.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12926..13528)
                     /locus_tag="U4A80_002014"
     CDS             complement(12926..13528)
                     /locus_tag="U4A80_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1975630.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3884 a         2944 c         2459 g         4285 t
ORIGIN      
        1 tattatacgt atacgcaaaa aagctaaaac attttttgtc tttgccccta caaaaatgtt
       61 tcagcttttt tcgtttttta ttcctgtatt tcttattttt cgaatgacag gatgattttc
      121 ccagcaggag gatgtccttc aactagctga tgtgcttgga tgacatgctt aagtgaagca
      181 gacagattca ttgaaataaa aacatgaaag gctccttttt ggtaggcttt gattatgcca
      241 gcaaaggctt ctgcatctag atattccttt cttggaacaa acgattcata aaagccttcc
      301 tttttttgcc gcaaatctag atctgggagg tcatttaatg caacatacct gccgcctggt
      361 ttcatgatct ggatgccggt ttctcctttg atacttcctt tcgtcgcatc gattacaagg
      421 tctgcctgat cagcaaattg cagacctgga tttgttttat cataagcagc aaatgctgaa
      481 gctcctaatt tttttacctt ttcctcattt ttactggatg cactggttaa gattcggatt
      541 cccttttcat ggagaagctg gatcagactt gacccgaccg cacctgatgc tcctagtatc
      601 attacagtat ctgtcggctg gatttccgtc aaatggttga ttagattata agccgtaatt
      661 cctggcgtga ccattccggc tgcttcttcc caactcattt tatctggaat ttttgctact
      721 tttgctgatg gcaacacgac ttcttctccg taagtcccgc cgactgcatg gacagctact
      781 cgatcaccta catgaacagt tgtgacatct gaaccaatct ctgtcacaat accagcgcca
      841 tcattacctg gtacataagg gaatttcaat gttcgtatct ctttcatttc tcctaaacgc
      901 aaggcaacat cataaggatt cacactgaat gcttttattt ctacacgaac atgtccagaa
      961 ccgacttctc tgggatgagc atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg
     1021 ttaattgcaa atcttcgcat ggtcaccatc tccctttttc tttaagttta cgtttatgcc
     1081 tttattttga caaaaaaaat gtcttttcgt tttggcgcat gattcaaaac aaaatagttg
     1141 tcttttttcg cgtaacgtgt taaattattt atctaaaagc gtaaagatta cgatttacgc
     1201 taataaaaaa taattggagg aatttgttga tggctaaaaa atcaaagatt gccaaagcaa
     1261 aaaaacagca agaaacgatt gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa
     1321 aagattacga aggcttgcgt aaacttcctt tagatgctcg tccagaacga ttgaaaaatc
     1381 gtgatttaat cgatggacgc cctcgcggat acatgcgtaa attcgggatg tcccgtataa
     1441 aattccgtga attagcacat aaaggtttga ttccaggagt aaaaaaagcc agctggtaat
     1501 ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac cactccttgg atcatcaaaa
     1561 aagatacgtt cgcttcttct agcagaaatg ctggtactag aagcagacgt atcttttttt
     1621 acgcacagct ggtccttttt tatatgtaaa ggctgcatgg ttacttcgct atatttattt
     1681 agtaacagga gtcagtgctt cgatacccac actaattttc aatgctcggt caacttctgc
     1741 cataatgggt cgatccaaat ggcagacctt ttctttcaat cttgatttat cgatcgttct
     1801 gatctgctcc aacaggataa cggagtctct ttctattcct gttccagtag ctttgatccc
     1861 aatgtgagtc ggaagcttag gtttagccat ttttgccgtt atggccgcaa caatgatcgt
     1921 cgggctaaaa tggttaccta gattattttg aataaccaaa actgggcgtg ttccgccttg
     1981 ctccgatccc acgactggag aaagatcagc aaaataaata tctcctcttt ttaccatagg
     2041 atcctcctta ttcttgatat tctcttggta tccgctgtcc taatccgcaa gccacttcat
     2101 agtggatcgt acctaattga tctgctatat cctgcatagt gatttctttg tctgcatttt
     2161 tgccgataag cgtcactttt gttccgacag gaaattcttg aggcaatcgg atcatcagtt
     2221 gatccataca tacacgccca atcgtttcac agtagttccc ttctaccaat agagaaaaac
     2281 cctgcatctt tcgtggccag ccatctgcat aaccgatcgg tatcgttccg atccactctg
     2341 cttcaggagt gatatatgtt tctccatagc cgattccttc acctgctggg agttttttga
     2401 cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa tggataaact tccggtagtg
     2461 cgtgcccaga gggattaagt ccgtacattg ctactccata acgaatcata ttgccgattg
     2521 tttcatcatg ccataaagcc gttgcactat tgctgacatg tacatatcga ggaagttcgg
     2581 gaagtttttt cagcacttct ataaagcgtt ctttttgcag attccaatat gtatcatctg
     2641 cctgatctgc tgtagaaaaa tgggtaaata aaccttccca ttctaataca cgactttctt
     2701 tgatgaatgc agctgcctct tttacttctt caggtgtaca aaatccgata cgacccatcc
     2761 cagtgtctgc ttttagatga atcgaaagag gcgtctctag cttacttttc tctaaaacag
     2821 cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac tgataaatca tgagcaacag
     2881 ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag gatgggctga gtaaatccag
     2941 cttctctcaa ctcgactcct tcatctaaat ttgaaacaca aaagcccgaa gctccacctg
     3001 caactgctgc ctcagctgtt tcaatagctc catgaccgta tccgttcgct tttactactg
     3061 caaacaattc ttttccttgc ggaagtcttg caacttcatt tgctacattt tcagttatag
     3121 cttttttatg tatcactgct ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt
     3181 ctttctagtc ttccaaaata atttgtgcaa aagctgtttc atttgtatga gtaatactga
     3241 cccatacatt gccttcatga ggtgaacgcg tcacaacagg ctgaccattt tcatttttta
     3301 aaatctcgac atcttgaaag gtaacttttc cgatcccagt tccccaagct ttagagaaag
     3361 cctctttgca agcgtaacgc ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact
     3421 ggaatagttt catttcatca gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga
     3481 tcagccgagt gatcctagga agttcgactg catcgatccc aattcctttt atcatatgct
     3541 tttctctttt caataaattt agaacactct cattctacca aattataacc attttgccac
     3601 aaataagtcc acaaataaca aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt
     3661 atgtttaatc gttatttgaa cggattacaa atccgcgttt tttatcattg tgacgtttac
     3721 cgccatcttt ttggctgtta ttatttttgc tataaccgcc ttttgatttg tttccacgat
     3781 aattaccgcc gtcacgacga ttacggttgt ttccgccacc acgtttgcca tttttattgt
     3841 atcctttttt ctgcattggc aatgggcgtt ctggtgtgat cttcactggc actgcatcag
     3901 ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa atcttgtgca gaatattctt
     3961 ctaataattt atctgctgtt tgcaggtatt tatctagacc attttctgca agttttgttt
     4021 cgatttgttc aactgcagca cctaattgac ctttgaatgc ttctttttcg gttggtggac
     4081 gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac atgcaagtag tccatttcat
     4141 ttggtgtaac gaaagtcaca gacataccgc cttttccggc acggcctgta cgtccgatac
     4201 ggtgaacgta gctttctgga tcttgaggga tatcatagtt gtacacatga gtgacacctg
     4261 aaatatctaa tccacgtgca gcaacgtctg tcgctacaag gatatctaaa tgaccgctct
     4321 taaatgaacg caatacgctc attcgttttt gttgagacaa atcgccatgg attccttctg
     4381 cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc
     4441 cgaagacgat tgttaattca ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt
     4501 tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa gtcagctgtc atttctttcg
     4561 ctttgatctt cacatgatgt ggatttttca tgaatttaac gccaatattc ttgatagctg
     4621 gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc aggaacttgt gaaatgattt
     4681 tttcgatatc ttccaagaag cccatattca acatttcatc tgcttcatcc aaaactaatg
     4741 tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg
     4801 tacccacaac gatatgtgga cgatctttca agccacggat ctgacggcca atatctgctc
     4861 cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc taaacgatat aattcttctt
     4921 gtgtttggat agctaattca cgtgttggtg cgataactaa accttgtaat tcgtgacgat
     4981 ctggatcgat tttttctaac attggtaatc caaatgcagc tgttttccct gtacctgtct
     5041 gtgcttgtcc aataacgtct tttccagcta atgctagtgg aatcgtttct gcttggatag
     5101 gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa caattctggt gataattcta
     5161 gttctttaaa tttcaaatga atcctcctat atggttgtta actgaggatg tagctttttt
     5221 gctaccccgt cgatttgttc ggtcaaagtg tattgtttct ttttgacagt ggccacgcac
     5281 gtttttatcc attcttagcc cctgtggcac caccgaaaaa tcactttgcg aagtttaggc
     5341 attagaaaag ccaaacgggt cgcgttcagc tttatcatta ttatgacatc tggcttcaac
     5401 aagttgtttc tatggcaaaa ccagtccaat ttctcttcat ctttcggcaa taggttattc
     5461 taacatagtt tttcgttttc tgcaagttta cagattttta tttcatttgc tgaagttgtt
     5521 ctaccacttc aaataagccc atgctgttgc tgccttttag cataatacta tctgttgctt
     5581 caagattttg tttgatagca tcaatcaatt tattcttttc tttttccaaa tagaacacag
     5641 gtagttgggg gtatttttgg ttcaattctt cttttagtgc atgcatttgt tccccataaa
     5701 ggaaaatcat ggcgaatttc tcaggatcga tatgttcact catttgcgca tgcatattta
     5761 aagactcttt ccctaattcc agcatatctg ctaacacagc gattctgcgt ccttttgttg
     5821 gcagattttt aaaagtatct aataccagtc ccatggctgt tggatttgca ttgtagacat
     5881 cgctcaaaat agctgcacca tttcctgccg ttagccattc ggttctgttt tgagtgatag
     5941 aaacatgggc gagcccttgg cgtatctcag gtatggacag tccaaagaag cgcccgatcg
     6001 tatacgcaat caaagcattc gttacattat aacttccagg cagcgggatc gtaaatgttt
     6061 ctccttctac tacaaaatcg gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat
     6121 cgccatttcc aataccgaat gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca
     6181 gcaacggttc atctgcgggc acaacgagta atccgtctga tacaagtccg tcagtgattt
     6241 ccattttggc tttagcgatt ccttctcgcg acccaagatt ttctatgtgt gcttcaccga
     6301 tcattgtgat tgctgcagct tttggttgag ccataagtga aagttccgtg atttcattgg
     6361 cgtgatccat gcccatttcc aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg
     6421 tgtaaggaag accaatatta ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt
     6481 cagacaagac agacgcggtt agatctttcg tggtggtttt accattgctt cctgtaattg
     6541 caacgacttc tgcgcctact ttttccaagt aataggttgc caattgctgc atggctttca
     6601 aaggatctgc tacaggaata actggaaaat tttttggagc ttgttcaaca ggcaagctcc
     6661 aaaatgaagc agatgctccg tttgtgatcg cttgctggat aaattcatgt ccatctcgcg
     6721 taccagcaag aggaacgaat aaacttcctt cggttatctt ccggctgtca aattcgacat
     6781 tggtaatctt tgtcagtgca gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg
     6841 ttagattcat tgagatctct cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc
     6901 gggttcgcat gactctcact tccaagtaaa tttctctatt cgtatttaga agctagaatg
     6961 aacgcggtta cgcaatcact ccagcctctt ctttatttct ctttattcat ttttttcaaa
     7021 aaaagattga cgctgatggt atcgattcat tcctaactgg atcagttctt caatcaaatc
     7081 accgtatttc aagcccatat tttcccataa gagtgggtac atactgaact ccgtaaatcc
     7141 tggcatagag tttaattcat tcaggaataa ttcattttta tttgtcaaaa agaaatcgca
     7201 ccggctcaat ccgcttccac ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt
     7261 ttgataaact tcttctggca cttcggctgg aatctgcatt tcgattttat tattgatata
     7321 ttttgcttca taatcataga atgctacgtc ttttacgact tcaccaggca aagtcgtccg
     7381 aacgtcttca tttcctaata cagcaacttc gatttcgcgc gcttcaattc cttgttcaac
     7441 gattgctcga gaatcatact gataggctgt tgctaaagca ttttgcagct cttctcggtt
     7501 ttctgctttt gtaatgccga cactagaacc catattcgcc ggtttgacaa acatcggata
     7561 aagcaaagaa ccttcacatt gatcaaatac ttttttagga ttttctttcc attgattctt
     7621 aagtactggt acataaggaa cttgcggcac accagcagct tgtaaaatat acttggtcat
     7681 gattttatcc atggcacatg cactggtcaa tacgcctgcg ccgacataag gcatattcaa
     7741 tgtctctaag aagccttgga tcgttccatc ttccccgttt ggcccatgta aaactggaaa
     7801 aacgatggct ccttcttctt tgatttcgcc cggattgatc acttttcctg taaagccttc
     7861 ctctgtctgt ccacttgggt cccatgaaag atgcaagaca tctttgctgg caggtttttc
     7921 tgttagtaat ggacctttga cccattgtcc ttcttttgta ataaatacga gttgaacttg
     7981 gtaataatta taataaatgg catttaaaac tgaaaatgcg gaaagaatgg acacttcatg
     8041 ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa agattgtcct cctgtattct
     8101 aaatcgttca gtgatttact ctcatatttt agcacaaaac aaaaataaac gaataactta
     8161 tttataaaaa aacgagccag cttctaaaag aacgagcgtc atttattttt tgtcagctag
     8221 attatttgtc ccgtttttta taaaaagtta tgataaagaa aaggagggat gatgatggcg
     8281 caaacaaatc cacgcagccg cttggcagaa atcattcgag tgttcatcaa ttacaatgta
     8341 gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta
     8401 ggccccacct tcatcaagat aggacagatg ctttctgttc gagaagattt gctttcttct
     8461 gccttcactc aaacattcaa aacattacaa gatagtgtac ctagcgacac attttcaacg
     8521 gtcaaaaaga cgatcgaaac agaactttct ctttcattat cagatatatt cgatgatttt
     8581 tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc atcgtgccaa gctgaagaac
     8641 ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg ccgaagagat ccgacttgat
     8701 cttcaactat ttgaacgagc gatccctttg atcaaataca ttcctgaaac tagcgtagtt
     8761 gatctaaaag gggtactcca agaagtaaaa cgttccttga tcaatgaaat ggacttctta
     8821 aaagaaagtc aaaatggcga acaattctat caaaagaaca atggctggaa agagatccgc
     8881 agccccaaga tttatgacgc tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct
     8941 ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga
     9001 attcaaaaca agcagctgaa acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa
     9061 caggtgtttg atgatggttt tttccatgct gatcctcatc cagggaatct gctattccat
     9121 gtactaacaa aagaagaaca gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa
     9181 gagttcggtt cctttgcttt tcgagcctcc acttctgcag aagaccctgt tgcgccatat
     9241 accatcaact acatcgattt tggtatgatg gggcatcttt ctgcaggact taggcaaaaa
     9301 ctcacacaag ccgttttagc gctgtatacg aaagatgctt atcgaatcga aaaagcggtc
     9361 cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc ggttccacca agaactgaca
     9421 agttttcttg aacagtatta tgattctcct attgatgaaa tcaacttgca agaagtattc
     9481 acacatgtcg tgacgatttg tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt
     9541 ttgctgaaag catttgggac attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc
     9601 ctcatggaag tcgcttcgcc ttttgctcaa cattattttc tcactcatct ggatgtagaa
     9661 gatacattga aacaaagcgg attagactta ttggaaggga tgaaggctgc gcctaagatc
     9721 cctcaacagc tccaccatct attagaaatg tggacttcgg gacaaggaaa agtcaatttg
     9781 gaattgaaaa agcaagacaa attattgagc aggatcgaaa gtatgatcaa tcggcttgtt
     9841 ttcggtatga ttttagctgc attgatcgta gggtcctccc tgcttgtcca agcagcacct
     9901 gtagaaaacg cagaggtcgt ttccttatta gggatattta cgtatgctat tgccgcattc
     9961 gtcattattt ttttggcaat cgatgcattg atccaaatgt ataagaagag gaaaaagtag
    10021 aaaaaggttg tgacagaaag aatcagcttc aagaaataag aaggggtttt acataaattg
    10081 tttctcaaat ttttgtgaaa attcggctta tttttcgaag aggctacttc tgaaacaccg
    10141 agaataaaaa atgtgaaaaa gctgttctgc acccagtaat aagaacaatc aaaaagggaa
    10201 catggaacac atttatcctg tttccctgtt ccctttttga tgtattcgag cataaataaa
    10261 ataccttcgt tacctactat cttctgtact tggtaaacct tagtcaattg ccttttttaa
    10321 cgcttgacga aaagacatat gatctttctc cgtaaagggt tttggaccct ttgtccttat
    10381 gccagctttt cttaactttc cactatgata acgttgttct agaagcgtgc cgatattcgc
    10441 ttcgtcaaat tcttttccag agtgatggaa cactcgcaca cccttcggca atacaagtga
    10501 agctaaacca taatcttgcg tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt
    10561 ataatccgca ctatcagctc cgcggtctac gtagacgatt tggacatgat tagcgtattc
    10621 tttggtcgta taatggtcca cactggtcac gataaccacg tcaagaccat aggattctcc
    10681 taattttatg acatcttctt ttactggaga accatctcca tcaatgaata gcctcattct
    10741 ttttcgcctt cttccataaa tgataaacag ttactaatcc gctcagtaaa agaccaacat
    10801 tgatccattt tgccaagata agcagttgta cactttctat cggcactgat tcatggttca
    10861 gcgtgatcaa taatagatgg attggctctc ccatttcttt tgctaaaaca aacaaaagcg
    10921 tcatcaataa aaactggtgg atcacactcc ctttattttg agtagtatac ttgacaacga
    10981 tccggcgatc cattggaata gctgtatcag tttgataata ttccttattt agccagctgc
    11041 ctgttactac gtgccagaaa ctaagcagaa agacacctat cgcaaaacca tttggaatca
    11101 agaggaccaa taaagaagaa ctcagtccaa gcaattgcat agccaatgga gaccatgtta
    11161 aacgtttcaa aatccatggt ccgactattt ttgctgcaat caagcctgct gcataaggca
    11221 aatacataaa aatcgaaagt ttttctaatc cgtacagtat gccaacataa aaactgctaa
    11281 ataaaaaaag gaaattccct agcattccat ttagaaaagt aaacagattt agccagcttg
    11341 gcaacttcca ttccttcttc tggtgtccta aaacaaacac gaacaagatg aacagcagca
    11401 agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca
    11461 aaagaaacaa taagccaaag aatatcaaaa ataaaacaag ctctcgcgta gctaccagat
    11521 ttcttttgat gtctttgaaa tccagttcgt aacgcggata atgactaacc gtatggtagg
    11581 ccatgacgta aaaaagtgta taaatagtaa agcttgctgc aatctgagtc ggtgcaggca
    11641 actcaatcgc ttgatacagg agaaatagcc agatagctgc aaataagtac tttttcccat
    11701 ccatattgat aaaaccttgc gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa
    11761 gccatgctgc actcagccct agcaaaacac ttgaaaaata aaataaagga aaataaaata
    11821 cgcctaatat tccaagcaat gaaccagcac caccaatcag cagagcgatc atcagcaacg
    11881 tgtaactatc tagaccaaat cgtagactgt tgactaaaaa tagtccagtc atccgaaagg
    11941 cataaaacag cgtaaatggc aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa
    12001 ataaaagata aggaataaaa ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa
    12061 aattacgccc aaatcgtttg agtttttcga acatgacaat ggctccttaa acagtcttat
    12121 aactaatctg ctcttcgatt ttagcattaa aggggaaata aaccaatttt acagaatata
    12181 ttcttgtaaa aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt
    12241 gattactcaa gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg
    12301 caggtgataa agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta
    12361 aaccagagtc cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg
    12421 cgagagcttt tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa
    12481 gttcttctgt aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct
    12541 gattcactcg caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc
    12601 gaaaaactga agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta
    12661 aataaatttt tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt
    12721 ttaataaaaa gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat
    12781 aagttttcag catgtaatcc acttgttttt gaccaatgat cggcacaaaa gcttccggcc
    12841 atacttcttg tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt
    12901 ccatacaccc acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga
    12961 gcatgatcat gctatacaca cccaccgacc atggtttcaa gaaaatcaca atctgcataa
    13021 attccctaaa agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa
    13081 agggaagcag caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca
    13141 attcatcaaa tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat
    13201 agtaacggac aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata
    13261 aaagcccaaa tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca
    13321 aaataggaat gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc
    13381 caaattgctg gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag
    13441 cataaagaat caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt
    13501 tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt catttattaa
    13561 aactgcctcc tt
//
LOCUS       DASJRQ010000074        13440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJRQ010000074 DASJRQ010000000
VERSION     DASJRQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.074"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(56..748)
                     /locus_tag="U4A80_002015"
     CDS             complement(56..748)
                     /locus_tag="U4A80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1975631.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1185..1754)
                     /locus_tag="U4A80_002016"
     CDS             complement(1185..1754)
                     /locus_tag="U4A80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1975632.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1828..2151)
                     /locus_tag="U4A80_002017"
     CDS             complement(1828..2151)
                     /locus_tag="U4A80_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975633.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2305..3117)
                     /locus_tag="U4A80_002018"
     CDS             complement(2305..3117)
                     /locus_tag="U4A80_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975634.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3471..4319
                     /locus_tag="U4A80_002019"
     CDS             3471..4319
                     /locus_tag="U4A80_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN1975635.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4464..5405
                     /locus_tag="U4A80_002020"
     CDS             4464..5405
                     /locus_tag="U4A80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1975636.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5395..7566
                     /locus_tag="U4A80_002021"
                     /pseudo
     CDS             5395..7566
                     /locus_tag="U4A80_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7678..9849)
                     /gene="copB"
                     /locus_tag="U4A80_002022"
     CDS             complement(7678..9849)
                     /gene="copB"
                     /locus_tag="U4A80_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1975637.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9869..12055)
                     /locus_tag="U4A80_002023"
     CDS             complement(9869..12055)
                     /locus_tag="U4A80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1975638.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12055..12264)
                     /gene="copZ"
                     /locus_tag="U4A80_002024"
     CDS             complement(12055..12264)
                     /gene="copZ"
                     /locus_tag="U4A80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1975639.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12277..12717)
                     /locus_tag="U4A80_002025"
     CDS             complement(12277..12717)
                     /locus_tag="U4A80_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1975640.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12791..13330)
                     /locus_tag="U4A80_002026"
     CDS             complement(12791..13330)
                     /locus_tag="U4A80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1975641.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3776 a         2815 c         2390 g         4459 t
ORIGIN      
        1 atagtccatt aaatagttca acttttgggg ttcagttcaa agaggggggg attttttatc
       61 gttgtggaat atttaaatac caacgcttgt catgaaaatc ttgtgctcca cccttagtat
      121 ttccttctgg atcgtttgtc gcacgcatca tgacgtatac tttcttatta gggaaatcac
      181 gcatattgaa agatacatga tagccaacat ttcctaaagt attataagct tgatttacgt
      241 ctggtctata aatcccatca gctcttactc tagctagttc ttttccagta ttgtagtcca
      301 taatgaaaat atactcgtat ttataattag caatgtgcca tccagctaca tgcaagtttg
      361 cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt tgttccatct gtcagcgtag
      421 aatttgcagc acctgctcga gttggatcaa ttactggttt atcctccgaa gtagttggat
      481 tatcatccgt aaaaccatga gccaaatcat aggctagttt ttctttactt acacccattt
      541 cagaaagata accgtaagga tctgtatgat cgccccaaat attttgtgtt acccataaat
      601 gcgatttgat tcctggttgg ttataaggag tgtctaatgt taatggaata ccatatttca
      661 ttgctgaatc tcttgccaat tcaacgtata ctttatagtt cttttcaaaa gtttctttat
      721 catgtgtatg ttgtaactcg atttgcacag gacttttggc tcacgaggtt gtcctactct
      781 acatcttgct gatagatatg ctgaaaggaa aacaccaaca ttatgttttg ctttttcagc
      841 tagaacgaga caagcaggaa tacctttaga agatatcaaa ggttttttag gtcataaaga
      901 tgtttccact acacaagtct atgcccatat ttctcccgaa gtgaaaaaaa gatcaatgaa
      961 tcaactagaa aactatatag aagagcaaat aaaaaagcac tcaaattgag tgctttttta
     1021 agctcactat cactaaaact atcactaatt ttatttataa gcgaacatgt ttctatcact
     1081 gattttttaa atctagcgaa aaacccttat cttaaaggcg ttcgttcaat tctttggcaa
     1141 ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt tttcttatag ctcatgtaga
     1201 aaaattttct tgatgatgat tttatccatc aattgagatt gactgttttg ctctcctatg
     1261 atttcagcta tgattgtccg attttgcggt tttcttctta actggtagtt gattttccca
     1321 gtttccatca actcgctaag catatccacg actccgccaa atccggtctt tttcgtaata
     1381 aaacctagtg ctaccattcg ttcatctaga ttttttgctg taatcgtcac accagtaatg
     1441 agcgagtgct gtaatgtttg ggccagctct tccatccctt cagcattgga tacgacggaa
     1501 gctgttttgt taataggttc ttcgtttatt tcttttggtt ctttcctctc cactttcact
     1561 gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt attgttcttg ggtcagcatc
     1621 acgccagcta ctttttcacg attaaatata tatacgccag ttcccgcgtc tcgcgcttga
     1681 gcaaatacat ccatcggcga tcgtttcact tctgtgatcg aagatgtggg aacttctaat
     1741 ttctttaatt ccaatggatt tcctccttta ctaatcgctt tttattatag cacagactga
     1801 cttgttttga tcttttttcc aaaggtttta tcggtttttg cctaaccagt aattgatatc
     1861 ggtaagaagt ctttcgatac caaatgaagg atggtacaga tacgtttttt gatcttcgtt
     1921 atggtctagt ggaaaaaaat gatcgatgat cactagatct gcataccgag gcggcttatt
     1981 tcgagtacat acatgtatag agatccgttt tcgatgtctt agcgttttgt tcagtacccc
     2041 agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg
     2101 agggtggaca tctaaatacc ctcgttcctg gagtgttttc agtaaaagca tcccccactc
     2161 tactgcgggg tgtgcaagtg cttgttggat cgctagccga aattgatata gtttatcttt
     2221 ggaattggga aatttttcta atgtttcctg ataataacga acttcttcta tcagccggtc
     2281 atggatacca attacgtaac gaagtcattc ttggtaaagt acttttgtca gctttgattt
     2341 gctttcgctc tcaaattcgc tattcagcca aaacagcgaa tcaagctttt cagtcacaat
     2401 cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc caccgctttt cagtaatctg
     2461 gttacagctc aagcgcaaaa ttgctagata atttttttct tttattggca aaatctgctc
     2521 ctctctaaat agcaattcag gaaacaattc aaagaatagt gttcgactgt ctcctaaaat
     2581 ctcgatttct gccggatcct ttctttgctt caataattgg atgaacatct cagaaaagga
     2641 ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata
     2701 tcttactttt ctttctgaac caaagatctg tgctttttta taagaaaaag aaaggtggta
     2761 ctcgccaaac cactttctca atcgctcttg cgtagaacga tcgtacttct gctcgtaagg
     2821 ttcattcaat agcagatttt tcagtaaaca aaacgggggt gattggatca gaaaaacaaa
     2881 caagagataa ggttcgctat atccttgatc ataaactaac tgaacggttt tcttatgaag
     2941 aatcagctga atgggacagt cgctttccgc caaacgtttc agatgccgat ccactgtttt
     3001 aacatcgatt ttcagttctt cagctaaatc agcttttcgc cagatcaaat ctttcattag
     3061 attgattagt gaaaataaat tctgtgcatt ttttgataat agtttttgcc atctcatcga
     3121 tcatttctca cctgatttct aaacatattg tttgatcaaa atgggttcac tcctaaaaag
     3181 ataaaaaact ccctttctat ttatttaaat acgcaaatct tcctcattat tttttatttt
     3241 actcaattta atcgtattct catttcaaaa cgaatgtact ttaaaaaaac ataataagat
     3301 cactttgctt gtaggatgta ttcccttcca ttacaatgaa ataaatagcg attttatgag
     3361 aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa acaaaaaatc gatcagttga
     3421 tataagaatt agaaaaagcg gtcatcggca aacgttcaac tatccaattg atgatcgtgg
     3481 ccctattagc aggtggtcat gtcttattcg aagatattcc cggtgtaggt aaaacgttac
     3541 tggtcaaagc actagcaaaa gctgtacagg gaaccttcag cagagtccaa tgtactcctg
     3601 atctgctgcc tagtgatatt cttggttttt ccgtatacaa ttcacaatca aaagagtttg
     3661 aatttcgtcc gggaccgatc tttaccacga tcttgttagc agatgagatc aatcgtacga
     3721 ctcctcgtac tcagtcagca cttttagaag caatggccga gaatcatgca acgatcgaca
     3781 acaatacata ccctctagac aatcattttt ttgtcttagc tacgcaaaat ccaatcgagt
     3841 acgaaggaac atatcctttg ccggaagcac agctcgatcg ttttttattc cgactacaaa
     3901 tcggttatcc ttcttttgat gacgaacttt tgctactttt ggataaattc gaaaaagtgt
     3961 tggataactt aaatgaagta ttgaacagtt atgaaataga agaaataaaa acaaatgtgg
     4021 cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt gcagttagtg tcagcaacaa
     4081 gaagtcatgc tgccatccag cttggcatca gtccacgagg aagcctttcc tttattcagg
     4141 cagcaaaagc ttatgcttta atccatggga gaaattacgt cacgcccaaa gatttgcagg
     4201 atttagttcc ttacgtgttt tcccatcgcc tgatttttta cgatcgttca ttgaaggaag
     4261 acgaaaagaa acaaatcatt catactcttg ttgcacaaat tcctattcct gtgaggtaga
     4321 ttgaggtaga caatggaaaa aatcaaagat tatttcatta attttgtgtt atttctggta
     4381 tatttcatca ttgtgctttt tgctcttgta ttcaacaatt ctttaggctg gttcttgttg
     4441 gcctttttta ctctttatgt atcatggagc ttgatttcat taagcagctt tctaaagaaa
     4501 ctttcggtta cttgtacatt gcagccactc gtttatcgca aaaaaagtat gcaggttatt
     4561 ttttccatag caaaaaagag aactttttct ttacctctcc ctcgtttaca aatcggcttc
     4621 ccagcaatct ttgcagaaaa aaacaagaga ttttgattct tacaaataaa ccgcaagaag
     4681 tccaattgct ttggcagcca aaagaaagag gatgccatga aacattaact gtcattttta
     4741 cagcctatga tagtctagga cttttcagaa aaagtgcaaa aagagagatc tcttttcctg
     4801 tgactgttct gcctgctttt tgtaagattc aagcagaaaa attacagaga gttctggaaa
     4861 aaaaacagca gctgaacgcg taccaaagag gtcttgattt tcgggaacat cgcgcttatc
     4921 gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc tgctcatact caagagtggt
     4981 tataccgtga atacgattat caagaagaag aatgttctcc tcttttttgc ttttggggtt
     5041 cgcccagtcc aaaatttgaa cctctgctag acttgtattt ttctttatgg catttgagaa
     5101 aagaaaagca gccggaactt ctgatattag gcaaccgcgc ctttcatggc aaagaccccg
     5161 gtcataccta ttttgcttct ttggaagctg gcgatgagaa agcttttttc gcacacttga
     5221 aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga gaattctcct gaagtctcag
     5281 aagctattga aacattgtca aaagatcgaa cggtttatct tgtttatttc gtagaaaaac
     5341 aactcatggt ccaagaaaaa gacaagatat gttcattgcc aggaggtgaa tggattgacg
     5401 attaaacggg aaaaatggct gcgtggcgtt tactcctttg tgatactaag tgtaggtgct
     5461 ccgatatttg tagtagctaa tcaattgcaa aacagctacc ttcttatcct aactattggt
     5521 gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg tacgtttgct gctttctttg
     5581 ccagtaatga taggttgttt gtttttatat tttccttcga agacattttc catattatgg
     5641 atcttacagt ttttttcgtc agtcacagag gaattcttcc atctatccca aggtaaactt
     5701 tattatttgc cagaaaagac agcttttttt ctcataatgt tgctgatcta tttatttatt
     5761 acattgaccg tggattacga ctattggtac gcaccctttc tttctttgat ggcttacttc
     5821 atgatgctta ccgtttttcg gaaaaaagat ttactgtacg aaatgattgc ggtagtaact
     5881 gttttgttcg tgtatctggc agcaaggcaa tttttctcca tccggttgtt gtcgggaaac
     5941 actcgattcc agtcattgac tacagttctg cttatttgtt tcttaacttt tagcgctatc
     6001 ttgcctttat atcttccgaa agtacaccaa tctctcgagg aaacgagtga accgatccga
     6061 gaatatctta atgatgaagg gctttatgac acactccatg agtatcaatt aaccggaagt
     6121 gcaagaacag gcttcagtga gaatgatgaa cagcttggtg gacctttata tgataatggc
     6181 gaagttgttt ttacagcgaa ccaaaaagga aatggctatt ggaagatcga aaataaaaat
     6241 cattacaccg gttctggatg gatagaagat caggacgcac ccatccaacc aatccaagaa
     6301 ttccctgttg acatcacgag ctaccaaacc gttatagaag acaaagaaaa cattacattg
     6361 accctaaaaa aacaagtttt cttaccttat ccttatggaa atactacctt gactgatttt
     6421 tcattagcta gtggacaaac gctttactat ttgcctgaaa gccagcgatt cattcctgtc
     6481 ccgaatgaat cgacacgttc tgcaacttta caaacagaaa ggttagtttt tacgagagaa
     6541 gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa atcagcagac aaatcttcaa
     6601 ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg tcgaattgac caaaaatgaa
     6661 gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt taaaaacaag cggaaatttc
     6721 cgctattcca aaacggatac cgggcatact ccaaaagaac acgattatgt ggattatttc
     6781 ctctttgatt caaaagtcgg ctattgtgac aacttttcaa cttctatggt cgtccttctt
     6841 cgaacacttg gtatcccagc tcgctggaca aaaggtttta caaatgggct cagaacaggc
     6901 acagataatc aagggaatga ggaatacagc attctcaata gtcatgctca ttcttggcca
     6961 gaggtatact ttcctggatt tggctggctt ccatttgaac caaccccttc atttgccaat
     7021 ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa
     7081 ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg agagatctga ggtcattcca
     7141 tcagaagaaa caacactcac accagcttat aaaatcgtct tattcagcct tggagtgtgt
     7201 ttatccgtct tgttgttctt tctattgtta ttcagaaaat ctctgcgctg gacaagttat
     7261 cttatctatc ttcatttttt tgcttctact aatacgattg catatcaaaa acttcttaac
     7321 cctttggaaa cattggtacc gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga
     7381 atagaacaag aattcaagct agatctgcgt ttcattcgat taactactca atatgaagca
     7441 gaaatatatg gacgacaagc agatccggta aaattcgata aaaaaaccta tttagaaata
     7501 acgcatataa taagaaaaat ggtatacaaa aaaattctga caaaagggag ccgcttcaaa
     7561 aaataagata aaatttctgg agaatctact tctggaatgc cgagaaccag ctgattgagt
     7621 atcgattact cgaaaaaaag gttgtgacac gttttttgtc acaacctttt ttcattatta
     7681 tctgattttc aatgtcaaag catttagagc tacaactacc gtacttaacg acatcaagac
     7741 agcaccaact gctggactta atatgatacc aattggcgca agaattccgg ctgctagcgg
     7801 aattgcaaga atattgtaac ctgcccccca ccagaggttc tggatcattt tctttcgcgt
     7861 ttgcttggct agatcaagaa aatgcaaaat atcttttgga tcgctgtttg tcaatacgac
     7921 atccgcagaa tcgattgcga tatctgtccc tgcaccgatt gccatcccga ttgaagcacg
     7981 ggctaaactt ggcgcatcat tgatcccatc acccaccatg attacttttt tcccttggtc
     8041 aaggtatttc tggacaacag cttctttatc atctggtaat aaaccgccat aatattcttc
     8101 gatccctaaa taatctgcta ctgcttgggc agcgttttta ttgtcaccag taagcatgac
     8161 aggagtgatt tttctttctt tcaatgttcg gataaattct tttgcttctg tttttactac
     8221 gtcacctaaa gcaacgattg cagctagatg attttctaaa atcaggaagc tgatggtatt
     8281 tccttgttct tggtaaggtt tcaataatgc ttgttctacg tttaatccga gacgctcaac
     8341 ttctttttca ttaactattt tcacttgttg ttttttgact gttgcctcta gcccgactcc
     8401 tgaaagattt tttagatttt cagcttgata aggcttgatt tcatgttctt tcaaataatt
     8461 cataatgcca acagccaatg gatgattggc attcgcttcc aaggctccta gatattgaag
     8521 gatttcattt ttattgaacg catcatcaag tacttcgaca cccgttacag taaattttcc
     8581 ttctgttagt gttcctgttt tgtccagcat gatcacatct agatcattgg cttgctccag
     8641 tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct attgatgtgg aacgagcaac
     8701 aactaatggg atagctaaac ctagagcatg aggacaggcg atgataaaga ctgttaccat
     8761 acgttctaat gcattaggaa gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc
     8821 cacaattaat gcgacataaa acagccattt tgccactttg tcagataatg actctagctt
     8881 ggatttttct ccttgtgctt ttcgcaccat ttccataacc ttcgctagat aaccattttc
     8941 accagttcca gttacttcga tttcgatcgt tccatcccca ttgatagaac cgccaatcac
     9001 tttgtcattg gcttgttttt tgattcctct tgattcgcca gtcacagctg attcgtcaac
     9061 aattgtttca cctttcaaga ttacaccatc tgttggcatt ttatcaccag accggacgat
     9121 caaacgatca ccttcgttta cttctttcaa tgaaactgtc tcttcttttc catctttcgt
     9181 caagcgtttc actgattctg gcagtaattc tgctagtttt tgtaaagcat tgctggcatt
     9241 cgacacagca ttcatttcaa tccagtgacc tagtagcata ataacgatca aagtagccaa
     9301 ttcccagaag aaatccatga cgtgtgtatg tggattgagc agatttgcga taaacgaata
     9361 gacactataa atatatgcaa ctgtaatccc catcgcgatc agtgtcatca tagcaggact
     9421 tttttgtttc aattccatct tagctccact taaaaatggc tgaccgccat agataaatag
     9481 tatagttgct aagatcaata cgagccaatc agaaccagga aacgttactt gaaatggaaa
     9541 ttccattccc atcattggtg aaaaaagaat gattgggata gctaggataa gcgatagcca
     9601 aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc atgccgccgt gttcgtgatc
     9661 catgtgcatc gaatggtcca tttgtttgct gtttgctata tctccatggt tatgtcccat
     9721 gtgcatggaa tgatccatct gactatggtc atgtttcatc tccatctgtt tttcatgaga
     9781 ttctgtactt tctttgttta ctacttcttt tttatgctca tgttttttct ccatatcgtt
     9841 cccattcatt tttactccat cccttctctt actttttcat gtgtcgattt aagcacaggg
     9901 agttcaacaa tacactgacg gaactaaacg ccatcgctcc tcctgcaatg attggattca
     9961 aaaagcctag tgcggcaaaa ggaataccaa tcgtattata gacaaaagcc cagaaaagat
    10021 tttgtttgat ttttctcaat gtcaacctag acaactcgat tgtttgtgca atggatgcca
    10081 aactgctatt catcagcgtc acatcggctg tttccatcgc aatgtccgtt ccacttccca
    10141 ttgccatacc gacatcagct aatgctaagg ccggtgcatc attgattcca tcgcctgcca
    10201 taccgacttt tttgccatct ttctgaagtt tttctacgta ggctgcttta tcctctggaa
    10261 gtacttcagc aaagatatgc tttggatcaa taccaacttg tttaccaatc gtttcggctg
    10321 ccagtttgtt gtctcctgtc agcataaaaa catctaatcc tttattctgg agctgcttga
    10381 ttgcttgttt gacttctagt ttgatttgat cagccacggc aatcaacccg atcacttgct
    10441 tttcatcagc taaaaacatg actgtttttc cctgctgttc taattctaac gcgtgttctt
    10501 ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc
    10561 gtactccgtt aatcgtaccg ctgattccag cacctgggtg ggctgtaaaa tcaatcatat
    10621 cgtatgttgc tgcttcttgc agttttccgt attccacgat tgctttacct agtggatgtt
    10681 cagaagcttg ttctaaagta taaaaaatag ataggacttc tttagaaccg actagatctg
    10741 taactttggg ctttccttct gtaatcgttc cagttttatc caaaacaatg ctgtctaagt
    10801 gagcagctgc ttctaatgct tctccgcctt taattaaaat tccatttcgt gcaccaacac
    10861 ccgttcctac cataatagct gttggagtcg ctaaacctaa tgcacatgga cacgcaatga
    10921 ctaatacaga gacactatgg atcaatgcga gttgccaatc accagtgatc aaacctgtag
    10981 ctatgagcgt gataaacgcg atgactaata cgatcggaac aaaaatccct gagatccgat
    11041 ctgcgatttt ttggattggg gctttgctcc cttgagcatc ttccaccatt tgaatgattt
    11101 gcgctaatac tgtctgcttg ccgacttgtg taacttccat gtgaagtaga ccattcgtat
    11161 tgattgtccc gccaaagagc gtgtcatccg gattcttttc tacaggaaga ttttcaccag
    11221 taagcatact ttcatctatc gcactgcttc ctgaaataat tcgaccatct gctggaatct
    11281 gctcaccagg acggatgact agttgatcac caacgactac ttcttcaatc gctaacgttt
    11341 cttctttccc gtttcttatc acttgggcgg tttttgtttg aagagacatc aattgtttga
    11401 ttgcattacc tgttttcgtt tttgctgtat gttccaaata tttgccgagc aaaatcagcg
    11461 tgatgatcat actgctgctt tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt
    11521 tgtaaatgct caatgcaaag gcagccgatg ttccaattgc taccaataca tccatatttg
    11581 gtgctttcgt cttgattgca tggtacgcac ctttataaaa acgagcaccg acataaaatt
    11641 ggacaggagc agacagcact aactggacga ttggaaaatg aaaaaagtga acaatcgccg
    11701 catggcttcc taacatcata gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac
    11761 tcaatataag atcgaatagc atttttttca aatacgcttg cttttcttct gcaatttttt
    11821 gtttgtgggc ttcatcgtac aaaattgcac catagccgat gttttctacg ctttggatta
    11881 atttttcagt ggtcgtccct tcatatttga cacttgcttt ttcagtggcc aagttgactg
    11941 tggcattgac cacaccaggt tgtccgttca gctctttttc gatcctagca gaacaatttg
    12001 cacaagtcat acctgtaatg acgaacgtat ccattttggt ttctgctgtt gccatcaaat
    12061 cacctctgct ttataaccca atccattgat tgcttgacaa atcttttcgg attgaacttt
    12121 tgtttcatca aacttgaccg taccgtttgc ttttttcaaa tttactttca ctttttgcac
    12181 gccgtcaagt gcgcttactg tttcttctac tcttgctgca caatgattgc agctcatacc
    12241 ttcgattgaa aattgttgtt tcattttttc ttcctcctat tgatgttgtt tacattcaca
    12301 ttgacctggg atacagttac attcgatcgt atctactggt tgcttttgtt ccagttgttt
    12361 caaaatctca ttgatatctt cttgagtcaa agtggcttct tctactagat ccgcaatcgt
    12421 ctctccaatc ctttttgcac agatatggga aaaaagattt tccgtcgcac tgcgaactgt
    12481 ttccatctcc gagacagctg gataataaat gaattttttt ccttcttgtt ctgtccagat
    12541 cacttctttt tttaccaaac gacccagcaa agttttgatc gttgctggtt tccaatccat
    12601 cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac
    12661 acgcatgatc tcccactctg aatcactgat ctttactggc gttttgactt tttccattta
    12721 tttcacacct ttcgtttaca aatgtaatca ttatcttatg aatacagatt acacttgtaa
    12781 acaaaaactg tcaattattt ttgctctcta ttttcaattt agttaaaaat tcttgtttct
    12841 ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt agctacttgg atcagctgct
    12901 gtattttcgg ttcattgttt tcttcaaaat gccagataaa agataaaaat tctaagtatt
    12961 ccctgtttag tttctccgaa aatgattcag gcatatctgg ccgagatttt ttgtttatct
    13021 ttctttttag agaacgccaa actactcggt agatggcgag ataacgcggc actttaagcc
    13081 aaatgatctt atctgcttct ttaatccttt cctctaatgt agaagtataa tttccttcaa
    13141 cgatccatga cggattagat accataaaca attgctgttc ttgcaaaaac cacttttttg
    13201 cctgaatact gtagtctgtt gtatgccata aaaggtctag cggcagtagc ggaaagtcgg
    13261 tgatttcttt gatcttcctt gcgagggtcg attttcccga cccactttgt ccaatcacga
    13321 tgatcttcat tcttctccct cacttctctt tggaatttgg ttcgatcata ccacttataa
    13381 cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa
//
LOCUS       DASJRQ010000075        13317 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJRQ010000075 DASJRQ010000000
VERSION     DASJRQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.076"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            282..2021
                     /gene="yfcC"
                     /locus_tag="U4A80_002027"
     CDS             282..2021
                     /gene="yfcC"
                     /locus_tag="U4A80_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1975642.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2178..3362
                     /gene="iadA"
                     /locus_tag="U4A80_002028"
     CDS             2178..3362
                     /gene="iadA"
                     /locus_tag="U4A80_002028"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1975643.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3473..3676
                     /locus_tag="U4A80_002029"
     CDS             3473..3676
                     /locus_tag="U4A80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1975644.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3777..4439)
                     /locus_tag="U4A80_002030"
     CDS             complement(3777..4439)
                     /locus_tag="U4A80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975645.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4452..5954)
                     /locus_tag="U4A80_002031"
     CDS             complement(4452..5954)
                     /locus_tag="U4A80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975646.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6502..8181
                     /locus_tag="U4A80_002032"
     CDS             6502..8181
                     /locus_tag="U4A80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1975647.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8308..8787
                     /locus_tag="U4A80_002033"
     CDS             8308..8787
                     /locus_tag="U4A80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1975648.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9006..9686
                     /locus_tag="U4A80_002034"
     CDS             9006..9686
                     /locus_tag="U4A80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1975649.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9694..11025
                     /locus_tag="U4A80_002035"
     CDS             9694..11025
                     /locus_tag="U4A80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1975650.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11040..12002
                     /locus_tag="U4A80_002036"
     CDS             11040..12002
                     /locus_tag="U4A80_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1975651.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12113..12451
                     /locus_tag="U4A80_002037"
     CDS             12113..12451
                     /locus_tag="U4A80_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1975652.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12530..13237
                     /locus_tag="U4A80_002038"
     CDS             12530..13237
                     /locus_tag="U4A80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1975653.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4274 a         2333 c         2904 g         3806 t
ORIGIN      
        1 gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct
       61 aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa
      121 agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta
      181 ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt
      241 tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt
      301 caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt
      361 ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca
      421 cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc
      481 aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag
      541 aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa
      601 aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga
      661 cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc
      721 ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg
      781 tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga
      841 tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta
      901 tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct
      961 ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa
     1021 tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga
     1081 cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg
     1141 tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt
     1201 gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta
     1261 aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa
     1321 agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt
     1381 tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa
     1441 tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat
     1501 tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag
     1561 ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg
     1621 atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg
     1681 gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg
     1741 ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag
     1801 atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta
     1861 tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg
     1921 aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa
     1981 ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata
     2041 aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa
     2101 aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa
     2161 agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact
     2221 taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag
     2281 aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc
     2341 cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa
     2401 atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg
     2461 gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag
     2521 gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa
     2581 aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg
     2641 gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg
     2701 cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc
     2761 acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa
     2821 ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt
     2881 gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt
     2941 tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg
     3001 aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt
     3061 ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag
     3121 ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa
     3181 ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg
     3241 atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag
     3301 gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt
     3361 aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg
     3421 ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga
     3481 tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata
     3541 cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc
     3601 tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa
     3661 agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa
     3721 ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa
     3781 acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct
     3841 acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg
     3901 aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct
     3961 acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct
     4021 cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt
     4081 cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct
     4141 atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag
     4201 acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat
     4261 acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca
     4321 gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct
     4381 ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc
     4441 aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa
     4501 gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt
     4561 gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc
     4621 gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt
     4681 ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc
     4741 tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc
     4801 atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa
     4861 gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta
     4921 agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg
     4981 ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta
     5041 tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat
     5101 agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca
     5161 aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata
     5221 ctgccgcttg tttctctgga ttcataaatt cctttgatcg tcatttcata agtggtatct
     5281 tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct
     5341 agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca
     5401 tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca
     5461 ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca
     5521 ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt
     5581 attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct
     5641 aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga
     5701 tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa
     5761 gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct
     5821 gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg
     5881 aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt
     5941 ttcataaaat tcatattttg catccctcct agtagatgca tttgataata gacaacctat
     6001 cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata
     6061 taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac
     6121 cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt
     6181 ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata
     6241 ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat
     6301 ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat
     6361 gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct
     6421 tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg
     6481 tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt
     6541 gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt
     6601 gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg
     6661 tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat
     6721 gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag
     6781 ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag
     6841 gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt
     6901 ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt
     6961 agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca
     7021 gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg
     7081 gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt
     7141 gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa
     7201 cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt
     7261 caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt
     7321 gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt
     7381 ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca
     7441 caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc
     7501 gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg
     7561 aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta
     7621 atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta
     7681 gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact
     7741 ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt
     7801 gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt
     7861 gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga
     7921 gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct
     7981 agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta
     8041 gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt
     8101 tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact
     8161 caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt
     8221 cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat
     8281 aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta
     8341 gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc
     8401 acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg
     8461 aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac
     8521 aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta
     8581 ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat
     8641 cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca
     8701 agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat
     8761 cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac
     8821 ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt
     8881 gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca
     8941 ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt
     9001 aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc
     9061 aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta actgcagaag
     9121 aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata ggagatatga
     9181 ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg
     9241 cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat
     9301 tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg
     9361 ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc
     9421 atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg
     9481 atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg
     9541 ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca
     9601 ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga
     9661 gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta
     9721 gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa
     9781 ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta
     9841 gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt
     9901 gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg
     9961 gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg
    10021 gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa
    10081 ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag
    10141 attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt
    10201 acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg
    10261 cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg
    10321 atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac
    10381 cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac
    10441 cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt
    10501 gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat
    10561 gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc
    10621 cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg
    10681 attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca
    10741 ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct
    10801 gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg
    10861 aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta
    10921 ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc
    10981 gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa
    11041 tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca
    11101 ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac
    11161 gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct
    11221 ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga
    11281 ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga
    11341 tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa
    11401 aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa
    11461 agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct
    11521 ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc
    11581 catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac
    11641 tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc
    11701 ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt
    11761 cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag
    11821 ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc
    11881 atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag
    11941 aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat
    12001 gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat
    12061 cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt
    12121 accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg
    12181 cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt
    12241 agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa
    12301 agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat
    12361 ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa
    12421 actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt
    12481 tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta
    12541 tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc
    12601 cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa
    12661 aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac agataaataa
    12721 tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag gtaataagat
    12781 gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc
    12841 tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc
    12901 agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg
    12961 tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc
    13021 attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca
    13081 aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt
    13141 atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg
    13201 cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc
    13261 gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt tggaata
//
LOCUS       DASJRQ010000076        13212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJRQ010000076 DASJRQ010000000
VERSION     DASJRQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.077"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1016
                     /locus_tag="U4A80_002039"
     CDS             147..1016
                     /locus_tag="U4A80_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN1975654.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="U4A80_002040"
     CDS             1225..2250
                     /locus_tag="U4A80_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1975655.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="U4A80_002041"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="U4A80_002041"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1975656.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="U4A80_002042"
     CDS             3333..5036
                     /locus_tag="U4A80_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1975657.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="U4A80_002043"
     CDS             5038..7071
                     /locus_tag="U4A80_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1975658.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="U4A80_002044"
     CDS             7082..7522
                     /locus_tag="U4A80_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1975659.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="U4A80_002045"
     CDS             complement(7722..8849)
                     /locus_tag="U4A80_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975660.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="U4A80_002046"
     CDS             complement(8852..9529)
                     /locus_tag="U4A80_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975661.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="U4A80_002047"
     CDS             9793..11559
                     /locus_tag="U4A80_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1975662.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="U4A80_002048"
     CDS             11574..12011
                     /locus_tag="U4A80_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1975663.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="U4A80_002049"
     CDS             12024..13175
                     /locus_tag="U4A80_002049"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1975664.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DASJRQ010000077        13211 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJRQ010000077 DASJRQ010000000
VERSION     DASJRQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.078"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4A80_002050"
     CDS             6..305
                     /locus_tag="U4A80_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975665.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(402..836)
                     /locus_tag="U4A80_002051"
     CDS             complement(402..836)
                     /locus_tag="U4A80_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975666.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1068..1778)
                     /locus_tag="U4A80_002052"
     CDS             complement(1068..1778)
                     /locus_tag="U4A80_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1975667.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1768..2628)
                     /locus_tag="U4A80_002053"
     CDS             complement(1768..2628)
                     /locus_tag="U4A80_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1975668.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2633..3271)
                     /locus_tag="U4A80_002054"
     CDS             complement(2633..3271)
                     /locus_tag="U4A80_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1975669.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3286..3585)
                     /locus_tag="U4A80_002055"
     CDS             complement(3286..3585)
                     /locus_tag="U4A80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975670.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3628..5112)
                     /locus_tag="U4A80_002056"
     CDS             complement(3628..5112)
                     /locus_tag="U4A80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1975671.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5132..5593)
                     /locus_tag="U4A80_002057"
     CDS             complement(5132..5593)
                     /locus_tag="U4A80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1975672.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="U4A80_002058"
     CDS             complement(5611..6678)
                     /gene="ulaG"
                     /locus_tag="U4A80_002058"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1975673.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6896..7666
                     /locus_tag="U4A80_002059"
     CDS             6896..7666
                     /locus_tag="U4A80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1975674.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7939..8766)
                     /locus_tag="U4A80_002060"
     CDS             complement(7939..8766)
                     /locus_tag="U4A80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975675.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9334..10569
                     /locus_tag="U4A80_002061"
     CDS             9334..10569
                     /locus_tag="U4A80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1975676.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11568..11828)
                     /locus_tag="U4A80_002062"
     CDS             complement(11568..11828)
                     /locus_tag="U4A80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1975677.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11855..13156)
                     /locus_tag="U4A80_002063"
     CDS             complement(11855..13156)
                     /locus_tag="U4A80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975678.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4187 a         2758 c         2080 g         4186 t
ORIGIN      
        1 aaaacatgga ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag
       61 aacatttcat gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt
      121 tttttcgtat aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca
      181 aaattaataa aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt
      241 tttatattag ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa
      301 aatagctgag cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa
      361 agaactaagc tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa
      421 agcacctttt ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga
      481 acaaatccta ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg
      541 atttggactg gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct
      601 attccttggc gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca
      661 aaatcagtga tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga
      721 gattcagatt gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa
      781 ccaacgctct tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt
      841 ttcttccttt cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat
      901 cacatataac tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga
      961 atgcatactt tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct
     1021 ctctatcaag tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag
     1081 tatgcatact ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca
     1141 catatattgg ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc
     1201 aaactgtttt ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag
     1261 acaagtactc cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc
     1321 acattccctg tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta
     1381 caaggaacct taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt
     1441 cctgcttgcg cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct
     1501 tcaaaagttt gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct
     1561 tcttctatta tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca
     1621 tatctgacac cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca
     1681 ttgccccaag tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca
     1741 cgttgcttca ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt
     1801 agaacttaag tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact
     1861 ccacatttca atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc
     1921 aagaaaatca acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt
     1981 tgttacaggg taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag
     2041 ttctcgagcc ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg
     2101 tagaaaaggt gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa
     2161 tggatcatcc atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct
     2221 ttcttttatt ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac
     2281 atcgtatcca gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt
     2341 tttcattagt tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg
     2401 ccgatgcgca cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct
     2461 cagctcagct atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc
     2521 aatcgacatc tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca
     2581 tgaaatatct ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc
     2641 agatacgaat gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg
     2701 taataccacg accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt
     2761 gtggatttaa tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct
     2821 tcgataaatc tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt
     2881 agattacctg ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc
     2941 cgtagagttc tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg
     3001 ttgcacaaca aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg
     3061 ttcctccagc gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc
     3121 gaatacagcg aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa
     3181 tatctactac atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat
     3241 ctaacgcaat ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg
     3301 gtaccacttt gtttcagcaa atttttcact gatttctttg actgacatca catttttgat
     3361 accgataact gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg
     3421 cgtaaaaact acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg
     3481 gattttggta attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat
     3541 caagcttgtt cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt
     3601 atgagtgttg tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata
     3661 gtcttctaca atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc
     3721 caataaacca atgacaacta cggcaattcc agcataacct aggactttca tgattactgt
     3781 aaacaatggc caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat
     3841 cccaatccat gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg
     3901 gaacaggcaa gcggctttga atccacctcg gttattggca taaactgcta taacggcatt
     3961 atcaaagaac aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag
     4021 taaaccaatc atcaaaaact gtcccaaagc accaaacaag accccaatcg ttaccgcatt
     4081 tggcgaacca aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat
     4141 cgtgtttgag attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa
     4201 ttgcaagatc gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa
     4261 actctgccct tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag
     4321 aatgatccca aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat
     4381 cgacaagaat cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt
     4441 tttcttttca tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa
     4501 catttgctga tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac
     4561 tgttaagtta gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc
     4621 ttgtatccta cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg
     4681 ttgaacctga acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg
     4741 gactaataag atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt
     4801 acggccaaat gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt
     4861 aaccatggca tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt
     4921 gaccaaacca ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc
     4981 agcgatgcat tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc
     5041 aatcaggtat gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa
     5101 aaattccccc atcttttttc tcctctatca attaattata ttctgccatc acaacttggt
     5161 aatcatcgat ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt
     5221 cggataattt ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa
     5281 acaaactagc cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca
     5341 acttggtaaa tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca
     5401 ttgctacttg aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct
     5461 cctccacata cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga
     5521 tagcttcacg ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa
     5581 agtatttcag caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt
     5641 ttcaatcgag aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt
     5701 tgtcggatag atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag
     5761 gcgatccttt ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga
     5821 ccaaatatcg tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag
     5881 catatcaaca gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa
     5941 acagacatcg attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc
     6001 tcctgcatga taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc
     6061 catctcctga ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc
     6121 tgtccgatca aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc
     6181 tggctttact gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac
     6241 ttcttttggc ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg
     6301 gatatctaga tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc
     6361 aaaaggatcg attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact
     6421 catacgcatc atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt
     6481 cccacaccat agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt
     6541 acagcccagc caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc
     6601 attcaagtat gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc
     6661 attgacagtt ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa
     6721 tattaatgtg tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga
     6781 ttttctttca gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat
     6841 gaacaactag tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa
     6901 aaactcactt gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac
     6961 tgaacaacta tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg
     7021 aagagattta aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc
     7081 ttataattac aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc
     7141 gattgccaga gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag
     7201 ctcgactgcg ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa
     7261 caacgtgaaa atcaattact ctccgcacaa agataattgt agttatattt taactggtgg
     7321 agagatgcgc tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc
     7381 agtcaatgca gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac
     7441 aacaaagata ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg
     7501 taagatttta gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga
     7561 tatttcagtc tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga
     7621 gatcagaaat acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa
     7681 atatgtaaat aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg
     7741 ctacgctcaa attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat
     7801 ctattgcttc ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa
     7861 aaggaggtat gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac
     7921 actgatttca caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt
     7981 tccgttgaaa cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc
     8041 taattgctta tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt
     8101 atgtttctta ttatactcaa tacccttctc acataattgg attgctttat cataatcctc
     8161 gattttacgg taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa
     8221 atcagtctca ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc
     8281 tccttttgac aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt
     8341 ttcggtcgtt tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc
     8401 ataaagatga tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact
     8461 tactttttcc tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga
     8521 attcaatgat tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa
     8581 tatatcggag atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg
     8641 cgacatattc ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa
     8701 ttgtacttgt gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt
     8761 aatcaaaaca atttccttcc ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa
     8821 aagttctaaa caaccaaaaa tgtttagaac agaataaaaa ttttaatttt aaaataatat
     8881 tcaaattaaa caactggcgt atctgctcct tgatttcttt ttgtgttatc tgaataatta
     8941 tcgtttacag ttataacaca tagtgaaaat tgtcatggcg agaaagtcta agaatatttt
     9001 taaacataac tatttctttc ttacgtgtgt ataatgacga cagcacacct atcaattaat
     9061 ataattattt tattttttta tgttctcatg tttattttta atgacacaac ttctcgtatt
     9121 tatctggcac aacatataaa tcgatcaaaa aattgttgcg tgtttttttg aaaattggag
     9181 tttcaacttg attacaaaat ttaaatttag aagttgtgaa aacagcgaga gacagtttaa
     9241 tattcaaaat atacataggc tactcgaacg ctgtgttttt tagtatacta gtaagaaacg
     9301 aagggataga ctcattccaa taaggaggat ttcatgaaga caattctttt tgctcctgca
     9361 gtattcaatt tagctgagac aacacgaatg attgaggttg ccaaagctat gaaagaccgt
     9421 tattactgtg tattctttgg cttttctaaa acttttgctc ctttgatcac agaaagtggc
     9481 tttgagtatc atttactagc gccgatcctt acgaaaaaac aagaagaaca aatcataaaa
     9541 tttgaccagt tgcgtacact taaaaaccct tttaccaaag aaatggttgc ctctcgtatc
     9601 actagtgaac agagtctgat tgctaagttt gagcctcgac tgattgtgac aggttcgaat
     9661 gtgacaattt ttttatcggc ccgcaataaa aagattcctt tagtatatgt taaaccctat
     9721 gctttgagtg aacctttttt tcaatcagag catgcgctga tgccccacga gttacgtttc
     9781 ttcggccctt taagcaatcc cctttggcac tggacaaaaa agcgtatagt aaatacccga
     9841 tggatcccca atgccttcaa acacgctgca caaaaagaaa tgcttccttt accaaaaaca
     9901 agtttacaaa tgatggatgc tgatctaaat ctcatcacaa cgcctaaact atttttggaa
     9961 gaagaacagc tgccggaaaa ttatcgaatc gttggtccaa tttttgctaa gctcagtact
    10021 tccttgcctg aagaagtgct aacttttata cagcgaaagc aacaagaaag aaaacgtatt
    10081 atctatttcg caatgggtag ttcaggcaat ccgcgtatgt tacgcaaaat tttgttcttc
    10141 ttacaaaaac aaccggatct agcggttatt gcacctattg actatctgct tgatgacatt
    10201 caatctgact cagaagatct tttaataaca gaatatctgt ctagtcatct tttgcatgaa
    10261 ttcattgatt tttcttttat ccacggcggg gaagggaccg tccaaactgc ctgtgaatcc
    10321 ggcaaaccgt ttatcggtgt ggggatgcaa tttgagcaaa gctgtaatat tgactattgt
    10381 gtaaaatacg gaaacgcaat cgccctcaca aaaccagtga ctgaaagaaa actgcagatt
    10441 gctctgcgcg aagtttcttc acccaagatt cgtcagcagg cacgcacgct gcaagccgtt
    10501 tttccaataa atggtccaca acaagcaatt caagaaatcg aaaccttcct gagcaaccac
    10561 ccaatatagt ttcaagtaga aaaagctttt ttaatagaga ctcatcagta atttcgctat
    10621 tcgagaccca gacagtgaaa atacaaaaaa agtaaatagt ggattgaaaa gttagagtta
    10681 cttaaattac ggtgtctaaa aaacacaaaa tcatccttaa aataaaagat ctgacgctat
    10741 ccaaatgagc ataggtagcg tctgccattt attcttagtc aaattctttg acacctttct
    10801 ctggttggat catctcaacg tttcttcaaa agaaagacca taataattag gcaaatacat
    10861 tttcttaaaa tgtgtcatat agatctagaa gcatcgcttt gtagagattt gtgccgctag
    10921 tcccctcaac aattactaaa tcaaatgtag gatttaccac tttatctttg ataatcaacc
    10981 tccctcttct gttgagacaa tgatctcgca atccgatgca tactcctata taaactgcca
    11041 gcaatcccca catattgagt agatcatgtc agaatgttct atgtcgtcag caacgataca
    11101 ctaaaagtca cgttcaccat gactgattgc tattcccagc acaattattt ctatacacgt
    11161 ttcatgattt gaataaacac tcacatcata catattacca ttcatacacc atactgcagc
    11221 ccctactata tggttgtaag ctcccaaatc tatatttgac ttgatcactt tggtagtaga
    11281 tctactaatt ctaaatccat tgctgataaa atttccattt tttcctcctt ctttctgcca
    11341 aaacagttaa aacaataata atctttatgt aaaaaagggc tgtgacaaaa gccttgtcac
    11401 agtcccatat tccgaataaa cggtggtcaa aagcttgcat ctgcggtaat aagttcaacc
    11461 aaacgaaaaa taagccaaca tttcacaaaa atttaagaag caattttcgt aaatttcttc
    11521 ttatttcttg aagctaacca cttctgttac aaccactttt agaacattta aagcttcgat
    11581 gtgacaaacc atctatgctg ctcgatcata aaattgatag caaaaattcc acaaagtaca
    11641 tatgtccccc atgaaaacaa aacattcata tgcggtacaa tgatctttgg aagaatcacg
    11701 atcaaaaatg atgccagtgc ccaaatccat cgattgattt gaatatgata tgtagctagc
    11761 atattcaata taaggtgact agccgttaat aaaaagataa aaacaaaaaa taaaatcagc
    11821 caatacattg tttcccacct tctaaaaacc aaacttagac agtctggact tcgttttctc
    11881 cacgactttg ttcatctgac agcatctttt tatcataagc ttttacaaat ggataataga
    11941 taataaatgc tatcaatgtg ttgatcatga tcaagataac aacacgccag tcatttcctg
    12001 ctgaaagata accgccgatt ggagctggta atgtccatgt tggcaaaaca gtaaatctat
    12061 tgaccatttc taatttgact gcaaaataac tgataatagt caaaactaaa ggaactaagt
    12121 tgaacgggat gaacatgtaa ggattcaaca tcataggagc tccaaaaatt atgggctcat
    12181 tgatattgaa gataccagga ataatagaaa aacgaccaac ctctttcaag aattttgatt
    12241 tacagaagaa tataaacaaa acagatacca cgatcgttgc tcctgcacca ccaattgtta
    12301 ccgtccattg ataaaactgt tcaggagcga tatagggtaa ttgggtggca gctgtaccat
    12361 ctgccaatgc ctttgcattc tcatccaaca tgatcagcca cattggacga acaacagccc
    12421 ctacaatact catgccatgg ataccaaatg accacaacaa atggatcaaa aacattggta
    12481 gtaaaacacc aagcaaatta ttccctaaaa tattggttaa aggctcgaat aacgataata
    12541 aggcagcgtt tacatcaaag cccgcgacgc ttcgaatcag ccaagtaatt gttaaaatga
    12601 ctgcacctgg aatcaatgcc tcaaaactac gggcaacagc aggtggcact tgttctggca
    12661 ttttgatagt aatatttcga tttttaaaga aacgatatac ttccactgca aaaatcatag
    12721 ataagatccc cgcaaacatc ccagatgctc ccaaagaact cataggtaag acccagccaa
    12781 gaggtgcctc ttcaggagtt aaaacgttta caggaacttg taacataaag aacgttccta
    12841 aagataatat accacctgtc acactatcta atccatatga tttagctaga tccgaaccca
    12901 taacaaaagt tgcataaata gccatcaatc ccatcgtcat cctaaagggt aaaacaatcg
    12961 ttgccgaata aggggcaata aattcattcc atccaggaaa cggtaaattg ataaagacca
    13021 taaaaaatgt cccaatcaaa atcaatgaca atgttgaaac aaccccatta cgaattgcta
    13081 ataaatgacg ctgtgtacca atttttgcca ttggtgggac aagtttctct tcgacccatt
    13141 tcatcagctt ttccactaaa accactcctt ttcaataaaa tatgaaccat tcattgcatg
    13201 acaacgcaaa g
//
LOCUS       DASJRQ010000078        12926 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJRQ010000078 DASJRQ010000000
VERSION     DASJRQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.079"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..3658
                     /locus_tag="U4A80_002064"
     CDS             131..3658
                     /locus_tag="U4A80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1975679.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="U4A80_002065"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="U4A80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1975680.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="U4A80_002066"
     CDS             7446..7787
                     /locus_tag="U4A80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975681.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="U4A80_002067"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="U4A80_002067"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1975682.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="U4A80_002068"
     CDS             9144..11564
                     /locus_tag="U4A80_002068"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1975683.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U4A80_002069"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="U4A80_002069"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1975684.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4393 a         2246 c         2739 g         3548 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaact
//
LOCUS       DASJRQ010000079        12900 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJRQ010000079 DASJRQ010000000
VERSION     DASJRQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.080"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            410..979
                     /locus_tag="U4A80_002070"
     CDS             410..979
                     /locus_tag="U4A80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1975685.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="U4A80_002071"
     CDS             966..1826
                     /locus_tag="U4A80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1975686.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /locus_tag="U4A80_002072"
     CDS             2016..2720
                     /locus_tag="U4A80_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1975687.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="U4A80_002073"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="U4A80_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1975688.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /locus_tag="U4A80_002074"
     CDS             4557..5873
                     /locus_tag="U4A80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975689.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="U4A80_002075"
     CDS             5874..6743
                     /locus_tag="U4A80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1975690.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="U4A80_002076"
     CDS             6798..7607
                     /locus_tag="U4A80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1975691.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="U4A80_002077"
     CDS             complement(7710..8339)
                     /locus_tag="U4A80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1975692.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="U4A80_002078"
     CDS             8509..9801
                     /locus_tag="U4A80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975693.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="U4A80_002079"
     CDS             complement(9877..10539)
                     /locus_tag="U4A80_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1975694.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="U4A80_002080"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="U4A80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1975695.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="U4A80_002081"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="U4A80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1975696.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4299 a         2224 c         2783 g         3594 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gttccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgacgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt gattttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aagaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
//
LOCUS       DASJRQ010000080        12746 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJRQ010000080 DASJRQ010000000
VERSION     DASJRQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.081"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4A80_002082"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4A80_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1975697.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4A80_002083"
     CDS             complement(323..892)
                     /locus_tag="U4A80_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1975698.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4A80_002084"
     CDS             1078..1980
                     /locus_tag="U4A80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1975699.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4A80_002085"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4A80_002085"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1975700.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4A80_002086"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4A80_002086"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1975701.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4A80_002087"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="U4A80_002087"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1975702.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="U4A80_002088"
     CDS             8554..8739
                     /locus_tag="U4A80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1975703.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="U4A80_002089"
     CDS             complement(8801..9997)
                     /locus_tag="U4A80_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1975704.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="U4A80_002090"
     CDS             complement(10167..11603)
                     /locus_tag="U4A80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1975705.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="U4A80_002091"
     CDS             11896..12705
                     /locus_tag="U4A80_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1975706.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4017 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aatttt
//
LOCUS       DASJRQ010000081        12593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJRQ010000081 DASJRQ010000000
VERSION     DASJRQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.082"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(67..1098)
                     /gene="queA"
                     /locus_tag="U4A80_002092"
     CDS             complement(67..1098)
                     /gene="queA"
                     /locus_tag="U4A80_002092"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1975707.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1291..2466
                     /locus_tag="U4A80_002093"
     CDS             1291..2466
                     /locus_tag="U4A80_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1975708.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2470..3111
                     /locus_tag="U4A80_002094"
     CDS             2470..3111
                     /locus_tag="U4A80_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975709.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3108..4025
                     /locus_tag="U4A80_002095"
     CDS             3108..4025
                     /locus_tag="U4A80_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1975710.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4022..4693
                     /locus_tag="U4A80_002096"
     CDS             4022..4693
                     /locus_tag="U4A80_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975711.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5234..5722)
                     /locus_tag="U4A80_002097"
     CDS             complement(5234..5722)
                     /locus_tag="U4A80_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1975712.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5923..6189)
                     /locus_tag="U4A80_002098"
     CDS             complement(5923..6189)
                     /locus_tag="U4A80_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1975713.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6189..6452)
                     /locus_tag="U4A80_002099"
     CDS             complement(6189..6452)
                     /locus_tag="U4A80_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1975714.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6928..7113
                     /locus_tag="U4A80_002100"
     CDS             6928..7113
                     /locus_tag="U4A80_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1975715.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7255..9078)
                     /locus_tag="U4A80_002101"
     CDS             complement(7255..9078)
                     /locus_tag="U4A80_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1975716.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9676..12381)
                     /locus_tag="U4A80_002102"
     CDS             complement(9676..12381)
                     /locus_tag="U4A80_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1975717.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3619 a         2616 c         2379 g         3979 t
ORIGIN      
        1 tttttctttg tcataaaatc tttgtaaagc ttttttgtta ttcaaaacta gcggcttttc
       61 ataaccttat ttcacgaaca tcgcatctcc aaaactaaag aaacgatagc gctcttcaat
      121 cgcatgctgg taagcagata aagtcaattc tcttcctgca aaggcactaa ctaacatgac
      181 taaagtcgat tttggcaaat ggaaattagt tgagaaagct tctaccactt tgaattcata
      241 gccaggcgta atgaaaatat cagtccatcc gctgtctgct ttgatttctc cattaaattt
      301 cgttccaatc gtttctaatg ttcgaattga agttgttccg acagccacga ttttcccgcc
      361 atttttacgt acttggttca atcgctcagc cgcttcttcc gtcaaacggt agaattcact
      421 atgcatatgg tgttcttcaa ttttatctac gctgacaggt cggaatgtac ctaaccctac
      481 atgtagtgtc agatagacta attcaacacc ctttgcttcg atctcatcaa gtaattcttt
      541 cgtaaaatgc aatccagctg ttggcgctgc agcagaacca ttttctttcg catagacagt
      601 ttgatagcga tctgggtctt ccagacgttc tttgatatat ggaggcaatg gcatttctcc
      661 caatgattca agaatctcta gaaaaattcc ttcatactca aagtcaatga tccgtccgcc
      721 gtgttccaat tcttctttca ctactgcttg caatctgcca tcaccaaaag agattcttgt
      781 cccaactttg gcacgtttcg caggtttgat caatgtctcc cacgtatctc cttgtgtatt
      841 cgttagaaga aggacttcta aatgcgcacc agtatctggt ttttctccat ataaacgagc
      901 aggcagaact cgtgtattat tcataactag cgcatcacca ggctgtaact cttcaatgat
      961 ttcatggaaa tgtttatctt ccatctctcc agttttccga tccaaaacaa gtaaacgaga
     1021 atgatcgcgg tcttttaatg gtgtttgtgc gattaattct tccggcaagt caaaatcaaa
     1081 atcttctgtt gttaacatat cgtcactcac tttctatcat gtgcttcatc attctatcat
     1141 gtttttcgtt ctgttggcaa aatcagcact ttttcacttc tattttttgt tttcctaaat
     1201 aaaaaaacta ccctatttgt attttttcat tgttttaggc atactagtaa tagatagttc
     1261 ttcattgaaa atagaaaaag aggtggcacc atgattgaat ttcaaaatgt gtcaaaaatt
     1321 tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt catttgaaaa gggtgagttc
     1381 atttgtttta tcggtacgag cggaagcgga aaaacgacga cgatgcggat gatcaaccga
     1441 atgactgatc caacacaagg gaaaattttg attgatggaa aatccatcca agaaattgat
     1501 ccagtggaat tgcgacgtca gatcggctat gttatccaaa gtattggatt gatgcctcac
     1561 atgacgatcc gtgaaaatat cactcttgtc caaaaattgt tgaaagtcag tcaagaagaa
     1621 cggaataaaa cagctgaaaa aatgattgat cttgtggaat tgccccgtga gatgttagat
     1681 cgctacccac atgaattatc tggtggtcag cagcagcgta ttggagtcgt tcgtgccctt
     1741 gcagcgaatc aagatatcat cttgatggac gagccattcg gtgcgcttga tccaatcact
     1801 cgtgattcct tgcaagacct tgtcaaagat ttacaagagc gtttgggaaa aacgatcgta
     1861 tttgtaaccc atgacatgga tgaagcgatt aaactagcaa gccgtatcgc catcatgagt
     1921 gaaggccgtg tgatccaatt tgatacacca gaaaatattt tacgtcatcc tgcaaacgat
     1981 ttcgtagaag agttgattgg agaagatcgc ctactacaag ccaagccaaa tgcgacaaga
     2041 gtcggcgaag ttatgctaaa tacagcaatc acgattacac cggaaaaatc attacaagaa
     2101 gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt tagttaccga taattcgaat
     2161 gtattaaaag gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt
     2221 gtcggcgaca ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat
     2281 accttgcaac gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac
     2341 aaagtcgtag gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg
     2401 ggagaagatc ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag
     2461 gtctgaccta tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt
     2521 gaacatcttt atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt
     2581 ggtgtcttac tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc
     2641 ttgcagacaa tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt
     2701 aaattacctg cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg
     2761 tacattggga tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg
     2821 actgagttcc aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct
     2881 ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc
     2941 gctggtggtt taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt
     3001 ttggcaggta cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc
     3061 ttggaacacc ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac
     3121 taaaaatgac catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc
     3181 ttgccagcaa tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac
     3241 agatcttagc aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa
     3301 tcatcaacaa tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt
     3361 caatttcagc ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg
     3421 aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc
     3481 aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt cgaaaagaca
     3541 ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag
     3601 tagctggtgt agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag
     3661 aaacttatgg cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg
     3721 cagtcgaagc aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca
     3781 gttatgatct agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc
     3841 cagttgtaga caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt
     3901 taggtcatac catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc
     3961 tagtggaacc atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga
     4021 agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa
     4081 tggctcctat atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct
     4141 ctttgcagca gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc
     4201 aaactgggtg attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc
     4261 cattttaatc attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta
     4321 ttccctctta ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat
     4381 ccttgatgtc ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt
     4441 accactctct gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg
     4501 gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg
     4561 aaccaatgca acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat
     4621 ggcgatcatc acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg
     4681 tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa
     4741 aggctttttt agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat
     4801 gggcaaagag ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc
     4861 caacgggttc atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg
     4921 ccaatccatt ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt
     4981 ctgcttgttc tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag
     5041 ccttttccat cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact
     5101 gccgtatcgg taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg
     5161 gctaaaaaca tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac
     5221 cggtacacga ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct
     5281 tcaccagaag agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat
     5341 tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg
     5401 aaagcgccac gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt
     5461 tggatacccg ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc
     5521 caaaagacca tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca
     5581 ttgctttttt caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg
     5641 cctcactttg gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa
     5701 ttttactgat tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt
     5761 ccattcctaa actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa
     5821 atcatgcaat aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg
     5881 agactctttg ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca
     5941 ggcagaataa atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg
     6001 tctattcagc ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg
     6061 cgtgggcata tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa
     6121 attcgtttgc ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata
     6181 gttactcatc aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct
     6241 ttttcgcggt ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga
     6301 atcgctctcc agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata
     6361 atctcgattt cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga
     6421 gcagtgctag gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt
     6481 atagcgtact tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact
     6541 tttcctcttt tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc
     6601 acttcttact cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc
     6661 atctttagca aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa
     6721 ggttcctcgt tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa
     6781 gatttgtaac ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat
     6841 gattgcctct ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc
     6901 gttgggtttg tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca
     6961 cttgacgagg ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag
     7021 gaagaagctg aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat
     7081 caagacatca aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg
     7141 ctgttcccat ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga
     7201 ccctctagct tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt
     7261 cttcaaatgg tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct
     7321 taaccgcaaa cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg
     7381 aggaatcaac acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac
     7441 ataacggata attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac
     7501 atctgggaaa tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc
     7561 tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc
     7621 gatatttacc cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg
     7681 tagttttgca tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat
     7741 cttataaaaa atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa
     7801 ccgatagaca aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc
     7861 aatcgatttg cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg
     7921 agataaagca aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga
     7981 gccatggaaa ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga
     8041 atagcctagt cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag
     8101 attgtatgca agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag
     8161 cgctgtagca aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat
     8221 ttgagacaaa acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt
     8281 gatccccacg aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt
     8341 ttttgctctt ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa
     8401 agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc
     8461 cgctcctgga agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc
     8521 aacaacgatc agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga
     8581 tgcagattcc ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga
     8641 aatagcgact tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc
     8701 tgagacagat actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa
     8761 gccagcattc tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata
     8821 agcatggatg atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc
     8881 ggcaatgggt agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat
     8941 ttgttccgta ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc
     9001 aaatcgggtt aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa
     9061 cctttttaag taatccaatt tctaaactcc tttacacaca attttacttc aagttttata
     9121 gtcgaaaatt tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa
     9181 actttaagaa aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta
     9241 atacagcaaa aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga
     9301 tagaacttga cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga
     9361 gatgtaaggc tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca
     9421 acctcttgat aaacggtgtt taaaagcttg catctgcggt aataagccca accaaattaa
     9481 aaacataaga agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt
     9541 acaacctctt ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg
     9601 caaaatatga ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt
     9661 tcacaacctc tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt
     9721 cattttttct tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa
     9781 accgatcatg atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc
     9841 ccgtaacaag tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt
     9901 ccctgagact tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat
     9961 aatagcgatc cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat
    10021 gaacgctaag gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac
    10081 tcctagtaag aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa
    10141 aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt
    10201 tcctaaatag tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca
    10261 tagtgcagta taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt
    10321 ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa
    10381 taatttgatg acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc
    10441 tttttggata ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt
    10501 cacagctgtt tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc
    10561 attttttagt gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg
    10621 aatgatcggt aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt
    10681 atccatattt ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat
    10741 ttctttttga tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct
    10801 gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat
    10861 cgtatcgatg acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat
    10921 accagcaatg atgccattga tattattaac aacggcatca atttgatcta gcaattcatc
    10981 aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag
    11041 attgcttagc aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt
    11101 tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg
    11161 ttgagatatt ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc
    11221 agtcagctta ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt
    11281 tgccacttgt ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc
    11341 atctttcagc tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc
    11401 taaatttttc acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt
    11461 tgcttcatcg attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc
    11521 tgctagttgc ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat
    11581 ttttgcttga tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat
    11641 ctcatctact ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat
    11701 ttcaggtagt tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa
    11761 attttcatct gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc
    11821 gatgtcgtaa cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa
    11881 ttcatttgtg atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat
    11941 cgcattgatt ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt
    12001 aaaactcagc agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc
    12061 ggaacggact ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt
    12121 atttttatga agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc
    12181 tgctggcttg tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc
    12241 ccataatgct ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat
    12301 cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag
    12361 cgtatttttg attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta
    12421 ccatgtattt catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata
    12481 tcacggcaat tgtctcattg taatctctta catattgaga aataattaag gattacagcc
    12541 tttactttat catgaaataa aagggaaaga taattttatg ccaaagtttt tat
//
LOCUS       DASJRQ010000082        12535 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJRQ010000082 DASJRQ010000000
VERSION     DASJRQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.083"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..771
                     /locus_tag="U4A80_002103"
     CDS             34..771
                     /locus_tag="U4A80_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1975718.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            785..1600
                     /locus_tag="U4A80_002104"
     CDS             785..1600
                     /locus_tag="U4A80_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1975719.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1613..2260
                     /locus_tag="U4A80_002105"
     CDS             1613..2260
                     /locus_tag="U4A80_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1975720.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2276..2932
                     /locus_tag="U4A80_002106"
     CDS             2276..2932
                     /locus_tag="U4A80_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1975721.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3094..3915
                     /gene="racE"
                     /locus_tag="U4A80_002107"
     CDS             3094..3915
                     /gene="racE"
                     /locus_tag="U4A80_002107"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1975722.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3918..5273
                     /gene="rph"
                     /locus_tag="U4A80_002108"
     CDS             3918..5273
                     /gene="rph"
                     /locus_tag="U4A80_002108"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1975723.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5274..5792
                     /locus_tag="U4A80_002109"
     CDS             5274..5792
                     /locus_tag="U4A80_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1975724.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5899..6399
                     /locus_tag="U4A80_002110"
     CDS             5899..6399
                     /locus_tag="U4A80_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1975725.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6679..7599)
                     /locus_tag="U4A80_002111"
     CDS             complement(6679..7599)
                     /locus_tag="U4A80_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1975726.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7764..8777
                     /locus_tag="U4A80_002112"
     CDS             7764..8777
                     /locus_tag="U4A80_002112"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1975727.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="U4A80_002113"
     CDS             complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="U4A80_002113"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1975728.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9862..10302
                     /gene="fabZ"
                     /locus_tag="U4A80_002114"
     CDS             9862..10302
                     /gene="fabZ"
                     /locus_tag="U4A80_002114"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1975729.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10417..11178)
                     /locus_tag="U4A80_002115"
     CDS             complement(10417..11178)
                     /locus_tag="U4A80_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1975730.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11367..12461
                     /locus_tag="U4A80_002116"
     CDS             11367..12461
                     /locus_tag="U4A80_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1975731.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4039 a         2175 c         2641 g         3680 t
ORIGIN      
        1 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
       61 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      121 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      181 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      241 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      301 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      361 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      421 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      481 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      541 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      601 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      661 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      721 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      781 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      841 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
      901 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
      961 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1021 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1081 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1141 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1201 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1261 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1321 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1381 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1441 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1501 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1561 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1621 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1681 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1741 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1801 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1861 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     1921 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     1981 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2041 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2101 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2161 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2221 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2281 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2341 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2401 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2461 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2521 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2581 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2641 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2701 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2761 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2821 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     2881 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     2941 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3001 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3061 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3121 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3181 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3241 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3301 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3361 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3421 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3481 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3541 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3601 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3661 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3721 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3781 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3841 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     3901 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     3961 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4021 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4081 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4141 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4201 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4261 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4321 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4381 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4441 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4501 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4561 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4621 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4681 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4741 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4801 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4861 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     4921 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     4981 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5041 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5101 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5161 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5221 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5281 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5341 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5401 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5461 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5521 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5581 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5641 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5701 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5761 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5821 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     5881 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     5941 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6001 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6061 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6121 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6181 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6241 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6301 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6361 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6421 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6481 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
     6541 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     6601 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
     6661 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
     6721 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
     6781 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
     6841 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
     6901 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
     6961 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
     7021 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
     7081 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
     7141 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
     7201 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
     7261 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
     7321 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
     7381 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
     7441 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
     7501 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
     7561 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
     7621 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
     7681 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
     7741 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
     7801 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
     7861 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
     7921 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
     7981 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
     8041 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
     8101 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
     8161 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
     8221 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
     8281 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
     8341 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
     8401 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
     8461 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
     8521 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
     8581 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
     8641 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
     8701 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
     8761 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
     8821 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
     8881 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
     8941 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
     9001 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
     9061 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
     9121 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
     9181 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
     9241 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
     9301 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
     9361 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
     9421 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
     9481 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
     9541 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
     9601 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
     9661 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
     9721 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
     9781 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
     9841 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
     9901 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
     9961 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    10021 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    10081 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    10141 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    10201 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    10261 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    10321 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    10381 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    10441 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    10501 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    10561 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    10621 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    10681 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    10741 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    10801 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    10861 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    10921 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    10981 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    11041 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    11101 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    11161 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    11221 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    11281 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    11341 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    11401 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    11461 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    11521 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    11581 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    11641 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    11701 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    11761 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    11821 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    11881 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    11941 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    12001 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    12061 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    12121 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    12181 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    12241 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    12301 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    12361 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    12421 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    12481 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatag
//
LOCUS       DASJRQ010000083        12200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJRQ010000083 DASJRQ010000000
VERSION     DASJRQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.084"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(162..326)
                     /locus_tag="U4A80_002117"
     CDS             complement(162..326)
                     /locus_tag="U4A80_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975732.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            621..1949
                     /locus_tag="U4A80_002118"
     CDS             621..1949
                     /locus_tag="U4A80_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1975733.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="U4A80_002119"
     CDS             2151..2984
                     /locus_tag="U4A80_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1975734.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="U4A80_002120"
     CDS             3000..3737
                     /locus_tag="U4A80_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1975735.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="U4A80_002121"
     CDS             complement(3892..4860)
                     /locus_tag="U4A80_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1975736.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="U4A80_002122"
     CDS             5092..5925
                     /locus_tag="U4A80_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1975737.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="U4A80_002123"
     CDS             5959..6798
                     /locus_tag="U4A80_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1975738.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="U4A80_002124"
     CDS             6824..7384
                     /locus_tag="U4A80_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1975739.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="U4A80_002125"
     CDS             7583..9304
                     /locus_tag="U4A80_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1975740.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="U4A80_002126"
     CDS             9469..10611
                     /locus_tag="U4A80_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1975741.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="U4A80_002127"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="U4A80_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1975742.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2048 c         2535 g         3462 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg
//
LOCUS       DASJRQ010000084        12163 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJRQ010000084 DASJRQ010000000
VERSION     DASJRQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.085"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            159..731
                     /locus_tag="U4A80_002128"
     CDS             159..731
                     /locus_tag="U4A80_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1975743.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            876..1580
                     /locus_tag="U4A80_002129"
     CDS             876..1580
                     /locus_tag="U4A80_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1975744.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1808..2146
                     /locus_tag="U4A80_002130"
     CDS             1808..2146
                     /locus_tag="U4A80_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975745.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2131..2985
                     /locus_tag="U4A80_002131"
     CDS             2131..2985
                     /locus_tag="U4A80_002131"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1975746.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3132..3365
                     /locus_tag="U4A80_002132"
     CDS             3132..3365
                     /locus_tag="U4A80_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEN1975747.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3563..5467
                     /locus_tag="U4A80_002133"
     CDS             3563..5467
                     /locus_tag="U4A80_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1975748.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5777..6139
                     /locus_tag="U4A80_002134"
     CDS             5777..6139
                     /locus_tag="U4A80_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975749.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6156..6767
                     /locus_tag="U4A80_002135"
     CDS             6156..6767
                     /locus_tag="U4A80_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEN1975750.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6869..7777)
                     /locus_tag="U4A80_002136"
     CDS             complement(6869..7777)
                     /locus_tag="U4A80_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1975751.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7819..8133)
                     /locus_tag="U4A80_002137"
     CDS             complement(7819..8133)
                     /locus_tag="U4A80_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1975752.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8432..10363
                     /locus_tag="U4A80_002138"
     CDS             8432..10363
                     /locus_tag="U4A80_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1975753.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10656..11207
                     /locus_tag="U4A80_002139"
     CDS             10656..11207
                     /locus_tag="U4A80_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1975754.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11520..12062
                     /locus_tag="U4A80_002140"
     CDS             11520..12062
                     /locus_tag="U4A80_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975755.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2556 g         3621 t
ORIGIN      
        1 gaatttaata gagaagcctg gcgtctatta agaaataatt ttcttgatga gcgtcaattt
       61 ttttaacgga aatctatcct atactaaatt cagtaaagaa aaaggaggaa tcaaagaaag
      121 ctattttaaa caaaccaaaa ttataggagg aataaatcat gactgaaaat aaagctactc
      181 aagaaagaac accccaagaa agattgcaag aagaacaaga acacaaggaa catgttcatc
      241 atacgaaaat taatgcagcg gccattgcgg atcatctttt aggcaacatg catacattac
      301 atgtgaaact gcaccaatat cactggtatg taaaaggagc caatttcttt acgttgcatg
      361 aaaaatttga agagttgtat aacgaaaatg aaaaatggtt cgacaaactt gcggagcgtc
      421 taattacttc tgggcataaa cctgcttcga caacggttga atttgaaaaa tattcgatgc
      481 tttcggaaga tgcagttaat aaatacgcta aagcagaaga catggttgaa aatattatcg
      541 aggacttcag aagcactcgt gacctaacta ttcgcgcgat tcgtttagct caagacgagg
      601 gtgacgatgc tttagaagat acattgattg cttttaaaaa tgacttagac aagaacattt
      661 ggatgctgca agcgttcatt ggtaaagaag cattagaaga tgatgacgct cttgatgagg
      721 atgaagatta aggtaaaagt agaagctgat ttagggattg gaattctttc cagtcctttt
      781 tccattttct tgatgtgcat caattttttt gtagtaagag acttatataa taaaagcagc
      841 gaatataaac aaaataaata aagggggaga atgaattgac taaacatacc catcatgaaa
      901 ttggcgacca cgcagcctgt atccgcttgg ttccaatatt caatcactta gaggatagcg
      961 cgatgaatta cattgctgaa aaagcccaca cgaaacagta tcaaaaagga gagtttctgt
     1021 ttcgttcaca agaaaaagag gatactttat atattatcaa tcgtgggaaa atccgtattt
     1081 atcgattggc agactctggc aaagagcagc tagtgcggat cttgaaccct ggtgatttca
     1141 ccggagaatg gaccttgttc aacccggatg cagtacacga ggaatatgcc gaagcaaccc
     1201 gagataccgt tgtctgtatg attcagcaaa aagatgtcca agattttctg gaacaatacc
     1261 cagctatttc tttaaaacta ttggcggaaa tgtctaaacg gttagaaagt tcagaacgtc
     1321 aaacgacaca agtagccgtg gagagtgtca ttacccgttt agttttgttt ttggcagaaa
     1381 atgtggaacc tgaaatgggc aactctccca ccatcaccct gccgatggca aaaaaggaca
     1441 ttgcttccta tttaggaacg acacctgaga ccatcagtcg caaatttgga gaattggagg
     1501 acgaaggatt gattcaacag ctgtctggga aaaggatcaa gattcaggat ttagatgatt
     1561 tattgcttta cagcgaataa tcggacacac ttttttctac acgttggtgt tctgtcgctc
     1621 ttcattaaca acgggacaga actcagtgct tttgcttcca tctgtccgtt tttggctttt
     1681 ttaagacaga acgaccttca ttcattggaa aaataaaagc acaaacatga taaccctcaa
     1741 gtttttttgt tttatttttc tttaagatag ggttgtcgaa caaaacgtaa aaataagtag
     1801 ggtgaatgtg cattgttttt gggtgatagt tgccatcacg ttgtcagcag cgggggcatt
     1861 ctttgtcgtc cctttcacca agaacacgat tggcggcgaa gcaaaaacgt ctaacgttgt
     1921 tgaggcagat aggttagcta tggaaaaaga agaggaagtt gtaaaaggag ccggcgtaat
     1981 cggacacgga aaagtggcgt attacgaagc gagtgaccag ggagataaaa gtgtattcac
     2041 tgtttggttt ccatggagct tagccggaat cttgttcagc atcaccatga ctagaatgac
     2101 gcgaaaaatt gaagaaatgg agcaaataga atgggattcg tattaggcgt tttgaatccc
     2161 catgtggtgg agattggttc tgttgtgatt cattggtatg gagtcattat tgcagcgggc
     2221 atattagcgg ctctccagtt gagcaccaag gaagcaaaga aaaaaggact ggaagaggat
     2281 accattatcg atatggcctt atgggcaatt ccgattggac tcctcggcgc tcggctttat
     2341 tatgtgttat ttgaacttgg ttattatctg caaaatccag gacaaatcct tgcgatttgg
     2401 aatggcggca ttgcgattta tgggggattg attgcaggag ggggtacgct atattggtat
     2461 gcaaagaaaa aaggtacttc tttggcactc gttcttgata ttttagcgcc caatgtattg
     2521 ttagcccagt ccattggtcg ttggggcaat tttatgaatc aggaagctca tggtgggccg
     2581 gttactcgtc aattcttgga aaatctttat ttacctgaat ttatcattga acaaatgaat
     2641 atgaatggta cctactacca tccgactttt ttatacgagt cattgtggag tttgttgggc
     2701 tttgtcctca tcgtcactat acggaaccga aaacagcttt tgcgtcaagg agaagtcgct
     2761 ttgagttacg ttttgtggta ctcagtgggt cggttcttca ttgaaggtat gcgaacggac
     2821 agtttatgga ttggcgagtg gatccgcgtt tcgcaagcct tgtctctggc cctgtttatt
     2881 ggcgcgattg tagtatggtt tatacgcaga caagattatc caccaatttc ttattattct
     2941 gatggcaata aggggcagaa aaagactatt aagatggtga attgagcaaa aagataaaaa
     3001 gaagtgcaaa acttgatatg cgtcaatttt cgtttgctaa aagtgagcta tactacagtt
     3061 gtaaagagga aaaggcagtg ttcattaatc tgctgccgat aaaataaaaa cacataaagg
     3121 agataggaat tatggaaaaa gctatattgc aattagaaac gttgtcttgt ccaagttgta
     3181 tgcaaaaaat tgaaggtgcc gtgaaatcag ttaacggtat tgataaagac agcattaaag
     3241 tattattcaa ctccagcaaa gtcaaaacca attttgattc agctgtcact tcgattgaag
     3301 agattcaaaa agctatcgaa aacgtaggct atccggttct taaagcaaaa gtcaaggccg
     3361 tttaacagat taaatgaact aggcaattct ggaagaaaag ctatttttcc aggattgctt
     3421 tttttgttgc aatccgattc gaggtgctta gaaacgataa aagagaactt tctaaacttg
     3481 atgtcagtca attaattact taggcaatta tcttatactg tgtttatcaa taaaagagaa
     3541 attaaatttg gagggaaaaa gcatgcaaca atatatatta agcaagaaaa atgttatcac
     3601 cgtcatcagt ggattgttaa tcgtactcgg tttcttcagt cactttgttt tagaaaacgt
     3661 aggactttca gagtggtctt tgatcatcgc ctctgtcttt gggattacgc caattgccat
     3721 tcaagcgttt caagcaatga aagtcaaagt catcagtatt gatgtcttag tcagtattgc
     3781 agcgattggt gcgcttttta ttcaaaatta tgaagaatcg gctattgtca cgttcttatt
     3841 cttattcgga cactacttgg aacaacgaac attgaaccaa acgcgatctg ctatcaaaga
     3901 attgactgaa atggcacccg aaagcgcctt gaaacaaatg gataatggcg aatttgaaga
     3961 agtagaagtg gatgatatag atgaagggga tatcttgctc gttaaaacgg gtgcgaaagt
     4021 tccagtagat ggcatagtcc ttacgggtga agggcatatc aatgaagcta gcattacagg
     4081 tgaagctatt ccggtcaaca agctagctga tgcagaagtt tttgcaggaa ccattttaga
     4141 aaacggaacg attcaaatca gagctgaccg agttggtgag gacaccacat ttggaaaaat
     4201 tattgaactc gtggaagaag cacaagattc taaatccgaa gcggaacggt tcattgatcg
     4261 cttttctaaa tactacacac cagctgtctt ggttctggca attgttgtat gggcgttcag
     4321 tcaaaatatt gagttagcaa tcaccatctt ggttctaggt tgcccaggcg cattagttat
     4381 cggagtgcct gtctcaaacg ttgccggtat tgggaatggt gctcgtaacg gtgttctttt
     4441 aaaagggagt gaagtcattc aagacttcag taatgtggat acaattgtat ttgataagac
     4501 aggtacttta actgtcggaa acccaaccgt tgcagcgact gaactgtatg aaaaagatcc
     4561 tgctgaaacg cttggctatc tggccagtgt ggaacgggaa tcagatcatc cattagcaaa
     4621 agcggtctta aatcaaattg gagaaacaaa cttttatcct gttgaaggaa ctgaagttgt
     4681 gaaaggcggc ggaatcgttt caagtgtagc tggacataga gtggctgttg ggaatgtggc
     4741 cttgatggaa aaagaaaatg tcactctcag caaaaaagtg caaaaagatg tcaaacggtt
     4801 tgaacaacaa gggaactctc tcgttttgac agcggtcgac ggtgaattaa aagtactgat
     4861 gggcattcgc gatcaagtcc gtccgggtgt gaaagctaat ttgcaggaat taaaagactt
     4921 gggcgtgaaa aatctagtcg ttctttcagg agataaccaa ggaaccgttg atgtggtcgc
     4981 cggcgaactt ggtttgaccg aagctcacgg ctacatgttg ccggaagaca aatcggctta
     5041 tatcggaaaa cttcaacaac gtggtcaaat tgtagccttt gttggagatg gcgttaacga
     5101 tagtccgtcc ttagctttag cagacattgg aatcgcaatg ggaagcggaa cggacgtagc
     5161 gattgaaaca tccgatgtcg ttttaatgaa ctcgaacttc agcaacttgc ctcacgcatt
     5221 aggattagta aaagccaccg caaataatat gaaacaaaat atcgtgattt caattggagt
     5281 agtgttggtc ttgttgacca gcgtcttctt cagcgaatgg atgaatatgt ctatcggaat
     5341 gttggttcat gaaggtagta tcttggtggt aattttcaat ggcatgagat taatgaagta
     5401 taagttgaaa ggtcgaaaag aacaaaaaga agtatcagca cctgcagaga aagtaaaaat
     5461 atcttaagaa taaattagaa gaggctgaga caatagtcca tccttgaaat gacaaaagcg
     5521 gaaactcaga tttttaaatc tgggattttc cgcttttttg agttcttgaa aaaaaataag
     5581 agtatagagt atgttcttta taaaattaat tttcgtgtaa atgattaaaa aatttaataa
     5641 taaaatttaa attcctatta agttgtaaca catgaaattg cttataatag tgtattgaaa
     5701 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
     5761 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
     5821 aaatgtaata aaaaaaaaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     5881 atgtttcagc gattcttcta ggattaaaat tttctatgct ttagaaacct ataaggaaat
     5941 gtgtgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     6001 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     6061 ttctttagcg aatgaagaca tcctttccgc tattcgtgtt tttttaaagt tatcagaaaa
     6121 tctatctatg aaatcttagt ttggaggaag ttattttgtt acaaagtatt ttttcagcta
     6181 tcgctgttta tatttctacc agtattgatt atttgtttat cttgctgatt atcttttctc
     6241 aaagccacac aaaaaaaggt attcgtcaca ttttttgggg tcaatatctt ggaacaggta
     6301 ttttggtagc cgtaagtttg tttgctgctt atgtgctaaa ttttattccg caagattgga
     6361 tcattggtct tctcggtcta attccgattt tcttaggaat cagagtcgct tttgttggcg
     6421 aagaggagga agaagaagag gaagtcgttg aaaaactcga atctagagga actaatcgct
     6481 tattctggac agttgcctta ataacgattg cttctggagg cgataattta ggtatttata
     6541 ttccatactt tacctcgtta gctttttcag aaatcattat tgctttaatc gtattcgcta
     6601 tctctgttgc ggttctctgc tatatcagtt acaaattagc caagatttct tttgtttccg
     6661 aaacgttaga aaaatacgaa cgaatcattg tccctataat gtttattgcg ttaggaattt
     6721 ttattatgat agagaacggg acgatacaaa cgctactagg tttttaaaaa tcataatcac
     6781 gaaagataaa aagagaggca gcgactaatc aaaatcgctg ccttttttgc ttttcaaata
     6841 atgaagtatt ttttagcgta gaactatctt acgtgttgtg ggagtatccc ttttcaagct
     6901 cttctttgct tgtagttatt tctattgtga tatgattctc tacgcttgat ttgtgtgcga
     6961 cttctgcaat ttcttctttt atttccctag ccttgtctat gtctgaaggg tctataagaa
     7021 ccgtgatgat aagggcgttt tcttccccgt ccagtgacca aatgtgaaaa tgtgagaggt
     7081 cttttacagc aggaacattt ttaatggagt ccgccaagct ctttacgttt acattttcag
     7141 gcgaaccatt caataaaatt ttcattgtgg tgaaaaattc aggaacggtt ttgtagagga
     7201 tataaagtgc aatcaaaatc gataagatag gatccagacg atatgcctcg gtaaagttca
     7261 ttagaatact cacaatcaag actccaatcc accctaatac gtcttccagc atatggaggt
     7321 tcaacatgct ctcgtttttg gatgtgcctt tgcttaaaat ccacgcagca tatccattca
     7381 atgcaatagc tacgagagaa agccagaaca tgccactata atttacgggt tgtgggtcaa
     7441 tcaaacgtgg cacactacga tagatcataa aaaacgagcc actaattagc acaacgccag
     7501 ttatcagcgc gcccagtaaa gaaaagcggt tatagccaaa agtgaaccgt tcatcttgtt
     7561 ttttctctga ataaccttga aaaaaccaag cgaatccaat tgaaatagag tcccctaaat
     7621 catgaactgc gtcggacata atggacacac tattgaaaag aaagccaaag aaaaattcgc
     7681 ttatagagaa aataagattt atgaagaaag caattttaat attctttttc ggttgattct
     7741 gattcgagtg attatggtct ttatctgatt ttgacattta aaaacctcct aagtttgtat
     7801 gctacatact aattggtttt atttttctaa ttcattaaca tgagttaaga cttgttcgag
     7861 aatactaaaa acatgaaggt catctaaact gtaaagcata ctttttcctt ctcttcttga
     7921 tttcactaag cgtgcgtctt tcaacgtttt taattgatga gaaatagcgg actgttccat
     7981 gtccagagaa atagcgatat ttccaacact acactctttt ttctgtaaaa gaaagagaac
     8041 cgacaatcgt gtaggatcac taatcacctt aaatagtttg cttattgaca gaatagatcc
     8101 tttatcgatt ggagaagttg tttttatatc catagtgttt ttattccttt catatgacct
     8161 tccgttcata tctatattac catatgagcg aaacaatgct aatcttttgt ttataaaaga
     8221 gaagtggcgt tctctttatc ttgacaaaat gtatatagcg atataaaatg agtatacaaa
     8281 catatgatta attagtcata tgtaaggtgg attcattacg atttactttt ggaataaacg
     8341 aaaggagtca gaaatagcag aaatttatgt gattcaagaa gtaaatgatg gaaaacaaag
     8401 gaattataaa ttatataggg aggcaataga agtggaaact atccaagaac aacagtctca
     8461 agaaaagcat aatcataatc acaaccacga tcacgaccat gatcatggga aaatgccagt
     8521 cgttttgtac tttgttggtt tagcattagc agtgattgct ctatttttga atgaagatta
     8581 tcagttggta caaaatgttt tgttctcaat cgctacaatc agtgccgggt atcacgttat
     8641 tcttcttgaa ggccttggag aaacaattga gaattcaaaa gcacaaaaaa agttcatacc
     8701 taattctcat attttaatgg gattagcggc aattggagct tctttgatgg ggaatttttg
     8761 ggaaggaaca ttattgattc ttattttttc tggcgcacat tttttggaag attatgcaga
     8821 aggaagaagc aaacgagaaa taacgaaatt actcgaaatg aatccaacga ctgcccggtt
     8881 aatcctgcct gatggtagta caaaaaatgt ggatgtcagt gaattaaaag tgggagatca
     8941 acttcaagtg ttgaatggcg atcaagtacc tattgatggc gtcattttgt ctggatctac
     9001 atccattgat gagtcgtcta ttactggaga aagtatcccg aaagagaaat ccaaaggaga
     9061 tggcgtcttc gggagtacaa tcaatggaac aggtaccttc acgatgaaag tcaccaaaga
     9121 aaacaaagat acagtatttt caaaaatttt acaattagtg aatcagaacc aagataacca
     9181 aacaaaagct gcgagtatta tccaaaaatt tgaaccgaaa tatgtcacag ttgttttaat
     9241 tgccattcct ctattcatgt tattggctcc ttttctactt gattggacat ggtcacaaag
     9301 tgtctataga ggattggtcc ttttagttgc agcttcaccg tgtgctttag cagcagctac
     9361 tgtctcggca acgctatcga cgacttctaa tttagccaaa aaaggagtcc tttcaaaagg
     9421 tagttcttac ctgtctcaat tagccgatat taaagcaatt gcatttgata agacgggcac
     9481 cttgaccaaa ggaaaacctg aagtaaccaa ttattatttt gctgattccg tgaacgaaga
     9541 aaacatgatt gatatcgtag tagctcttga aaaagaatcc aatcacccct tagcagatgc
     9601 tattctaaga aagtttgagc aaaaaaacaa actaactatt gaagtagaaa accaaattgg
     9661 taaaggattg acgggagatt acaaagggag aaattatcgt ataggaaaac cgacttcgtt
     9721 tgatgatgtt tcagatgaat atagtcgttt aaataatgaa tgggcgtcag aaggtaagac
     9781 agtcgtatac gtagcagtag atgaagaggt tataggcctt attgccctta tggacgttcc
     9841 gagtgaatat gctaaagaaa ccattgaata ttttaggaaa caaggtatac acaccacatt
     9901 aattactggt gactcagaaa tgacgggaaa agcggttgct aaacaattag gaatagatga
     9961 agtgattgcg aatgtcatgc cggaagacaa atccagaatt atagacgaac aaaaagaaaa
    10021 atacggcgtg accgccatgg ttggagacgg tgtaaatgat gctcctgctc ttgttaatgc
    10081 agatgtggga atcgctatgg gagatgggac tgatgtggca gtagaggtat ctgatttagt
    10141 tttaatgcaa aacaatttat ctaaattggt acaatctcat aatatttctt cgaaaatgaa
    10201 ccgtgttatt tggcagaata ttattttttc aatggccgtt gttgcttttt taattgtcgt
    10261 tagtttctta ggcttaacgg atattgcaat cagtgtcatt attcatgaag gaagtacttt
    10321 ggttgttatt ttgaatggac ttcgattgct aaaggcagtc taaagaatga ttgcacaact
    10381 tcaaattaat cgagtaatag agataattaa ggattacact acatttttta tctctattac
    10441 ttgtttatat ttatctagat gttcaaagtc aaaatgatat atcaaccata ctcttgtggc
    10501 gcgggttgaa tataagatac atgaaattgg ataaaatcat gtatcaagaa tcctatcctt
    10561 atagggttct ttttttttac tccccctaaa cacacgattt cgtgttgtaa taactataat
    10621 catgtattcg aacagactaa aaaggaggga ttgtcatggg ctataatgtt cagccactac
    10681 ggtcgcaaca ggagattaat gattttttat tttgcctgcg acggaataaa aatgcggaac
    10741 gtgacgtttt cttgtttcta attggaatta acagcggtct gcgcatgtcc gacatcgtta
    10801 aactcaagaa aaaagacgtg attacctcaa aaaacccgcg catcgtggag caaaagacag
    10861 ggaaaacacg cattttatac ttaagcagct tacaggactt gattcacggc tataccgcac
    10921 ccttagatcc ggaggcttac ttgtttccca gtactaaggg cggccattta gaagtcaata
    10981 cggtctacca gatgtttcaa aaggtcgcga agctgttagg aagggacgat atcggcaccc
    11041 acaccctacg aaaaaccttt ggttaccact attacaagaa aaccaaagac gtggccacct
    11101 taatggaaat ctttggtcac agcagcgaga agattaccaa acgctacatc ggaattaatg
    11161 aagatgaaat cagtgaaacg ttattaaatt ttcggttagg tttttgattg tattatataa
    11221 tagataagca cagtaagacg gttataatca cccacagtat tggacaaatc aacctttttt
    11281 tggtatatta tatatacaaa tgagagcagg ctcatagtca acttgatata aatccggaac
    11341 ataagggtcc ggcgggtggc gagcaccaaa actaaactcg atacaaatcc ggaacgtaag
    11401 ggtccggcgg gtggcaagca ccaaaactaa acctgtacaa aaggctgctc ttttgagcag
    11461 ctttttttat tgggaaaaag gagcattctt cgcacgcggc aaaaaaagaa tacaaacgca
    11521 tgtctataaa agagacaacc caaattacca gacagttaaa cgcgatttat aaggctgctc
    11581 atctcttaca agaacatttt gtagataaaa aagtatcctt tgtcggagaa gtctcaacgg
    11641 tagcgattat tttttcgaca actaatttta tgcacctctg tggcattgat tatcgcagag
    11701 gcacacactt gttttttcaa gatgccttag acaggaaaat aaatttacag gatattcaaa
    11761 tcaaaacgga tgggacgacc tttcagaaat tacaagtaat aggtagcctt gatttattat
    11821 tggggaagca tatttctatt gttggcagag gtgtttactc ttctcttcgc tatgatgcag
    11881 ctattcgaac aaggaaaaaa atattggctc tgtctttgaa acaaaacgga ttaatctata
    11941 tccctatctc tttgttaaac ctatcatcaa aggaaattgg acctggacaa aaagttactg
    12001 gcatttttag cgaggattta acatccgggg aacttaaaat gattatggaa gtcatcgatt
    12061 aatcattaat agtgatatgc gacgtcattt tgaagtaatt attctatttt gggttataaa
    12121 ataaagcatt ttaagacgat ttcaaatcat tcatgtgggt tat
//
LOCUS       DASJRQ010000085        11841 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJRQ010000085 DASJRQ010000000
VERSION     DASJRQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.086"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..2377
                     /locus_tag="U4A80_002141"
     CDS             5..2377
                     /locus_tag="U4A80_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975756.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="U4A80_002142"
     CDS             2438..3898
                     /locus_tag="U4A80_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1975757.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="U4A80_002143"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="U4A80_002143"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1975758.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="U4A80_002144"
     CDS             6129..6278
                     /locus_tag="U4A80_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975759.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="U4A80_002145"
     CDS             6353..6946
                     /locus_tag="U4A80_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1975760.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="U4A80_002146"
     CDS             6959..7858
                     /locus_tag="U4A80_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1975761.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="U4A80_002147"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="U4A80_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1975762.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="U4A80_002148"
     CDS             8724..8984
                     /locus_tag="U4A80_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975763.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="U4A80_002149"
     CDS             9429..9635
                     /locus_tag="U4A80_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1975764.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="U4A80_002150"
     CDS             9683..9886
                     /locus_tag="U4A80_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975765.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="U4A80_002151"
     CDS             9902..10339
                     /locus_tag="U4A80_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975766.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="U4A80_002152"
     CDS             10332..11039
                     /locus_tag="U4A80_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975767.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="U4A80_002153"
     CDS             11419..11598
                     /locus_tag="U4A80_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975768.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4430 a         1967 c         2463 g         2981 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta a
//
LOCUS       DASJRQ010000086        11577 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJRQ010000086 DASJRQ010000000
VERSION     DASJRQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.087"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            381..587
                     /locus_tag="U4A80_002154"
     CDS             381..587
                     /locus_tag="U4A80_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1975769.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(789..1790)
                     /locus_tag="U4A80_002155"
     CDS             complement(789..1790)
                     /locus_tag="U4A80_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1975770.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1795..3708)
                     /locus_tag="U4A80_002156"
     CDS             complement(1795..3708)
                     /locus_tag="U4A80_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1975771.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3876..4382)
                     /locus_tag="U4A80_002157"
     CDS             complement(3876..4382)
                     /locus_tag="U4A80_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1975772.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4542..4982
                     /locus_tag="U4A80_002158"
     CDS             4542..4982
                     /locus_tag="U4A80_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1975773.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5008..6165)
                     /locus_tag="U4A80_002159"
     CDS             complement(5008..6165)
                     /locus_tag="U4A80_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975774.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6168..6524)
                     /locus_tag="U4A80_002160"
     CDS             complement(6168..6524)
                     /locus_tag="U4A80_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975775.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6822..7796)
                     /gene="sgrA"
                     /locus_tag="U4A80_002161"
     CDS             complement(6822..7796)
                     /gene="sgrA"
                     /locus_tag="U4A80_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1975776.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7992..8831)
                     /locus_tag="U4A80_002162"
     CDS             complement(7992..8831)
                     /locus_tag="U4A80_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1975777.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9016..9903
                     /locus_tag="U4A80_002163"
     CDS             9016..9903
                     /locus_tag="U4A80_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1975778.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10497..11191)
                     /locus_tag="U4A80_002164"
                     /pseudo
     CDS             complement(10497..11191)
                     /locus_tag="U4A80_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11175..11573)
                     /locus_tag="U4A80_002165"
     CDS             complement(11175..11573)
                     /locus_tag="U4A80_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1975779.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3451 a         2303 c         1735 g         4088 t
ORIGIN      
        1 aaaaattttg gtgaattaag tatttccttg ataacactgg cttttggcct cattttaggt
       61 tatcatttgt aattttattt cacagttgat atgattgtta tgtaaaataa aaaagtttag
      121 gatacatcga atttttgggg aaattctttt gggggtatcc taaatctttt ctattatatt
      181 cccatatttt ctattattgg tctagacgta tgatggtaaa ctttgtacct atctgattgc
      241 tgaatcatta attgcttatc attagccatc tttgtttcaa gttcgctaga tgatcatttt
      301 attcacatcc taacaaactt gtcacattta tagattctta atctatttat catacaatta
      361 taaaaaagga ggtgctgctt atgaaagcta tccatatttc tttgcttttt ttcgctagtg
      421 cattgatttt atttgctatt catctttgtt cccagcctta tatcaataac aacggaatat
      481 tagtggaacc agcattcttt tgcttaccac tcggcttttt gtcccttctt gcctccgcag
      541 gcttcggatt tgttgctttt tttaaacgat caaaacaaca aatctgactt ataaaattta
      601 cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa atttcgaata aaaggtgccc
      661 cttgccatct ctaactcctc agtcacaagt tacaatattc ataaaccatg aaaagcttgt
      721 ttattgaaat tgctccttgt gacttggagc taagtgacag attacaacct cttttgcatc
      781 ataagacttt actccttgaa aaacattact aacttttgat atgtttcttg cgcttgttta
      841 ttgctgaaat caaactgata ttcatggttc aaaacagcat ttttctccgc ataatctact
      901 tttgtgactt tcacccccgc tttctcaaat acagaagtta cttcttccat ttgtttcgta
      961 aacgtatttg tatttccatc cgttaagaat gtgttttgcg tccaacgatc cgcatgtctg
     1021 accaaggcta aacttttgac cttctctgat tgttcaaagt ccttattatc aaagtaagcc
     1081 catccacatt tttcataaag aaagctagag attttatcac ttacttgagt aagtgcagaa
     1141 ggatccaaca gaccagacaa agaaataaac ttcgtaatct gcttgttctc caaagtcgga
     1201 tcaagatgat cagctttttt aatttcttca ttttgaagtg ctaacaaata ttctccacta
     1261 atttgtgcac ctgccgaatt tccaccaatc gaaatattcg accaatctgc ttgatctttt
     1321 atctcttttt tagctgcttg aatggcttcg ttcaattgga taactggtgt aggatgtttt
     1381 gcttccggtg atcgttcata atctatattt acgatgattt gctgcgtgtc tgaacataga
     1441 tattccaaat aatcacgaca atcattttta tcccctccta catatgcgcc tccatgcatc
     1501 cagaaaagaa tcggtaattt tttcttttcc actttcggtg tgtagatatc catattatta
     1561 tttttgtact ttgaaggata ggatatttct ttttttatat cgatttgttc gattttttgc
     1621 tggaaatcat ctggtcgggc atatacttgt tctttttcac ttgtgtcaaa ttgttttcta
     1681 acaagcaagc taatcggctt tggtgtgatt tgattaatca aaaatacagt gactaaaata
     1741 actacgatga taactggtac tgcaatcact attcgcttga accatttcat tcatttaccc
     1801 tctctttctt cgccaaatcc atgtgataat cgtgctaaca gataaaaatc cacctgaata
     1861 aatgagaagc gaatgtttct tttctccagt tttcggcaac attctttttt ttgcatctgt
     1921 ctttttttgt actttcactg tcatctgaat cggttgcgta tcatcacttg attgatcctc
     1981 tcgaggttca ttctttccct tattgtctgc attctctgtc ggatcttgct tttttggtgg
     2041 agctactggg atttttttgt aatagaaaat aatagttggt atcgtctcat taaaaatccc
     2101 ttcagtcggt ccttcaacat ttgctaattc gtatccttcg aatgtctttt gctctgtcat
     2161 ataattttct ccataagctc cgtaaagctg gatttcgtcc tggtctttga gtagactttt
     2221 attgttttca tccacatact ttacgtttat ctgatcatgg aacactactt tcaaaaagtc
     2281 cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt gcctttccat gccctgctcc
     2341 ttctactaat aaaagatctt tataccctcc tgataactca tataattttt gcgcgttaga
     2401 cacaggtact acatcatctg ctgttccatg gataatcaat ttaggcaatg aatctgacat
     2461 tccaatcgta ggtagagcag cttttgtatc caaatgcatc tcctgatcaa aatagtattg
     2521 tttcaaaagg cccataaata aatcttcatg ctcttgatcc ccaatgatgt ctaactgatt
     2581 ttttatggca tccggtagtg cgggggctac tgtctggctg tacgtaaaac gtagttgttc
     2641 atcaattgat gcaaatccgc aattttcaat aattcctttg acattgacag gaatatcttt
     2701 cgctaataca gacatcatcg tagctgctcc catcgaacca ccataaagta aaatcttttg
     2761 agatggccgc tctcgaactt cttgatttat ccagtaaagg acgtcatctg actcatattg
     2821 tccataggtc acataatcgc ctccgctatt accagtcgct ctggaatcaa caaataataa
     2881 attatacccg gccttataaa aatcattgta ttcttctgta acgatcccgt tattccaatt
     2941 ccctcgaaat ccgccatgga tcatgactgt tttatctgaa ccttgatcga cataatatgc
     3001 atatacatcc atcattttgc catcttgttt gtttagtaca gtttgatgaa gttgttttgt
     3061 atttggatag aagcctgtgt tttctgtagg cgttgtccca tcatctgtac ctccaagcat
     3121 cgacatcaac acactgtttt ttgcaaaagc gatatcgtac aatagataag tagcaatcag
     3181 atgtttgata atctctttat ctacaccata atcattaaca gcaagctcag tgactaataa
     3241 ctctattaat tttgacttga tgtcttctgt tggtccatta agtatttctg tcatctgttt
     3301 accgaagtaa ttcaaatatt ctttaccatt gaaattttca cctttaccga tatcatcatt
     3361 ttctgtccca ttctctttgt cacttacttt agcagaagag gattcgtcgt ttgacatttt
     3421 tgtagctgtt gcttcttctg tttgttggtt atctgtggta gcggtcgttt cttgatctgt
     3481 gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt tctgtttgtg aagtactcga
     3541 tttcgtttca tttccagcat cagtagtttc tgtggtttct acctcccgag attccgattc
     3601 ggaagaaatg ctctctccat atactgtcca ttgcgggacg cttgtactta cagcaaataa
     3661 gatgaccaac gctcccttta atacttgttt tgttctcaag ctattcactc ctttttatgt
     3721 ttctaggaga attatatgta aacgcaaaca ttttttatac aaaaaataaa tggtttatcc
     3781 cataaaaata gaacaggatt attttctcat tttacgtaat aaagcagaat ggataaacca
     3841 gtatccattc tgcttttttt aaacgaagaa acaacttatt gctccaaaaa tgtcagaaat
     3901 cgtttatccc aaattttctc gtaaaaagcg attgtttcct ttgctttcat aaaaggatta
     3961 tcataagttg tcgttgctcc tctacacatc ataagctggt atcctaatcc atgagcaaac
     4021 ttcacagtcg tatctataca atactctgct tgtgccccac ataattcgat cgaatgacag
     4081 tccaattcag ttaataaaac actcaaatta gtcttataaa atgaattcgc atgggttttt
     4141 ctcacaaaat aactgtgctc aggaatagat aattccgaca atagctccca tggttttgtt
     4201 tgtggcacta attgagcatc ttcgtgttga acgaaaataa ctggtttccc aatcgtttcg
     4261 tatgttttga ttcttttatt cactaatgct gtcactgttt ctaattcgtg tatcggcgaa
     4321 gaatcaaaac ataccccttt ttgtaaatcg atcactacta ttgcgtcggc tgtgtttgac
     4381 atacttgtgc cttctttctt ttttcttgat ttaatagtat ttcttatcta gctagttcca
     4441 ttatacgtga caacattttc ttattttaca cctctaaatg gcacttttcg actatttctt
     4501 catgttatta tttcataaat acttagctat tggaggaatg tatgatgatt aaattgtata
     4561 caggttctgg ctgctcgtct tcaagacgcg caaaacgttg gcttatcgtc catgatatac
     4621 cgtttatcga acagaactta attcgtcaac cactaaccaa aaatgaattt atccacttac
     4681 taaaattgac aaacaacggc gtgaccgaaa tgctctctac tcattctatt agttataaaa
     4741 atcttgttca cagaatagat gaactcagtt tgagtgaact atatattgag attcgtacat
     4801 ccccttcttt attgaaaaaa ccgattatct acgaccaatc tgcaaaactt ttgactggat
     4861 ttagcgaaga taaaatcagg atgttccttt ctaaaactaa aagaaagcaa gagctcaacg
     4921 aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa taatcttttt gcaatgtctt
     4981 aattttggca taaacaatct gcttttctta attaacgaaa tccaattttt ccaatatgga
     5041 tctatttttc tcattcctga tttttatgat ttcagctttc agacgcgccc aatcattttt
     5101 tgtaggttgt ccattccagt aaaatatctg tgtattcgtt tgatcatatg cctttttgtc
     5161 gataggataa tcgctaatga caatatcagg taactgatca gcggatgcac tatattttat
     5221 tgggcgttca gtcatagata atatcctttt ctttgcccat aattcctgtg ttttaccata
     5281 ctttgattga atatagaata tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa
     5341 catacagtat tgaaaaatca accgtctgtt cttttcataa taagtattaa gctctggaat
     5401 tctgcgagct accccactaa aaaactgttg catgattgga tacatcactg gaaatatatg
     5461 ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc accttctttt ttgtcccaaa
     5521 aacccttgaa tacaaatgga tcattgccaa attgacatat agataatttt tttctgaagt
     5581 tgtaaaacaa atcttgaagt ttttttcaaa attttctaca atagacgatt caatccagcc
     5641 ccaactttct tgtactttat ctggagtatc caacaaatct tcttccaaat aggtgttcat
     5701 tacaccaaac ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca
     5761 gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta aactcttctt ttgttatcaa
     5821 tggatgcaca aatttgttca tctcttctgg gacatacgta atcgagtggc cttgtttcat
     5881 tcgttgcaac gaaattctta gtaggctgat ccatttcttt ctatctatac tacgcgtata
     5941 tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta ttttttatac ttgataatat
     6001 atcagaatga ttttcgtcga atgactgcca atacatcaag tgaaaaaagt atcggatacg
     6061 gtattcttct ccttctatcg gattaccttt tttcaagctg caagataaat gatagttttt
     6121 caaaatagta ttaagttctc ttaattttct ttgaatcgta cgcaaactta tataattttt
     6181 tgactaagat tttctaagga ttgataagta cctttgtaaa gtgaatccag taaaatataa
     6241 ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg agaagaaaac
     6301 tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg atacctttcg
     6361 caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta gtttagatct
     6421 aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata gttcaaaaat
     6481 tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca tactcctttt
     6541 tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata gattcgttac
     6601 tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt gctcttattt
     6661 tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct
     6721 ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt cactcaaaaa
     6781 tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac tcgatggaag
     6841 agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat ccatgtaatt
     6901 agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt atctgtttct
     6961 ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt tagcacactt
     7021 tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg taccaaatca
     7081 agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt gctactctca
     7141 ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc
     7201 gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct gctcgtgttt
     7261 tctgtgctaa tttcactgct cgtactctct gtactgcttt caccgctcgt gttttctgta
     7321 ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt ccaactgctt
     7381 ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa atcaacttta
     7441 tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt atactcaata
     7501 ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc tgaacttcca
     7561 ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac gcttttgttt
     7621 ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg atcagttttc
     7681 acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat tccaatcatt
     7741 gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt cttcatttct
     7801 atcactctcc atataattac cttctatttg ataaaggaat tatataaaat gaaaatgatg
     7861 agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg cttgaatttg
     7921 tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat cagccttgct
     7981 ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt tttatatgca
     8041 tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat agatgcttca
     8101 gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt ggagaaaaaa
     8161 ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt ccaattccaa
     8221 gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag tgcgctgaaa
     8281 aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg attcaatccg
     8341 tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc aaatctaagc
     8401 atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac tgcaataaat
     8461 aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa ccgatacgcc
     8521 cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac aacgagacct
     8581 cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc tattcttttt
     8641 ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg tccgaccgta
     8701 ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag tgcattattg
     8761 acaagaattc ctaaaacaaa accaacaaag actattaaaa cagatccaat caaccgaata
     8821 atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt ctttcttgtc
     8881 tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac gaactaagaa
     8941 tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg ttagactgtt
     9001 tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat gtgcctttca
     9061 gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat tgcaacgttt
     9121 aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa atcaaataat
     9181 caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata cggaaaagag
     9241 atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact cggtccaaac
     9301 tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa actctttaaa
     9361 cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga taaagatcta
     9421 gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct attttcgact
     9481 aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt ctccaagctt
     9541 gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat gaattttgat
     9601 tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact aaaaaacggg
     9661 gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag ctattatcta
     9721 gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa atctgagcta
     9781 gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa aacaatccgt
     9841 aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa tatattcaaa
     9901 taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt ttggaaattc
     9961 cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt tttttatttg
    10021 taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa aaagtttttt
    10081 atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc cctatttttt
    10141 cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg aaaagttatt
    10201 ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt atactagaga
    10261 aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt tattcaaaac
    10321 tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc gtttaaatga
    10381 gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata ttgaatcgtc
    10441 agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa attgattcag
    10501 aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt ttgcacccaa
    10561 tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac ttgatctagt
    10621 agctctttac ctaacgttat cgctgagctc cagatcgaat ggctgtagtg acttgttcac
    10681 aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg atctcagtcc
    10741 gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg ttataataag
    10801 gtgcaatata atctgctcca ttattaatag ctaaataccc ttgtaattct gtgtaaatag
    10861 ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga aggcctgctt
    10921 gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata actcgagcat
    10981 cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga atctgtccga
    11041 ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct tttttcagaa
    11101 ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa taaatacttt
    11161 ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg atcctcttga
    11221 tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc tacatacatt
    11281 gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg tcctaattca
    11341 ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg tttatcaaaa
    11401 cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat cttataacaa
    11461 taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt ttctttcctg
    11521 aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt catctct
//
LOCUS       DASJRQ010000087        11460 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJRQ010000087 DASJRQ010000000
VERSION     DASJRQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.088"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..240)
                     /locus_tag="U4A80_002166"
     CDS             complement(<1..240)
                     /locus_tag="U4A80_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975780.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFP"
     gene            534..1349
                     /gene="thiD"
                     /locus_tag="U4A80_002167"
     CDS             534..1349
                     /gene="thiD"
                     /locus_tag="U4A80_002167"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1975781.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1678..3129)
                     /gene="cls"
                     /locus_tag="U4A80_002168"
     CDS             complement(1678..3129)
                     /gene="cls"
                     /locus_tag="U4A80_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1975782.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYRDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3470..6136
                     /locus_tag="U4A80_002169"
     CDS             3470..6136
                     /locus_tag="U4A80_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1975783.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6500..7252
                     /locus_tag="U4A80_002170"
     CDS             6500..7252
                     /locus_tag="U4A80_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1975784.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7274..8428)
                     /locus_tag="U4A80_002171"
     CDS             complement(7274..8428)
                     /locus_tag="U4A80_002171"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1975785.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8613..11054
                     /locus_tag="U4A80_002172"
     CDS             8613..11054
                     /locus_tag="U4A80_002172"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1975786.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11208..>11460)
                     /locus_tag="U4A80_002173"
     CDS             complement(11208..>11460)
                     /locus_tag="U4A80_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1975787.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3702 a         2027 c         2436 g         3295 t
ORIGIN      
        1 tggaaattct ttttcaatca gttttttcat ctgtttatga aattcttttt tttcagtaaa
       61 cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa tagattattc ctttttctgt
      121 gttttgcgta taagctttta tactcctgtc ttttttgaaa gaacagatcg tatagccatc
      181 tatttggtct ggtagtcgtt tgattttctt caaaatgact tttttatcct caagtttcat
      241 ttttttcacc tgactttatt tttttcatat ggaaatcata gcgcctttgg ttatatgaaa
      301 acaagaagga tactgaaata tatataaatc aaatagtggt tatttattat tagaatagcg
      361 aatacaaata aacgaccact attttctgat ttattgcaaa tgttttttca aataaatttg
      421 attaccaaga aaaggtagtg gtatatttac tgagggatac tttgaaaaac atatgataga
      481 aacagtaaca gtattatgaa aaatcttttt ataaaaatgg aggaatcgat ttgatggaag
      541 atcagtatgt acaagcaatg acgatcgcag gatcagatag cgatggaagc gcagggatgc
      601 aagcagatat gcatacattc tttacaagaa atgtttacgg tgtttctgtc atgaccgcat
      661 gtgttgcagg taattcttac ggaatcggag caagtgtgac attgccaaca gattttatcg
      721 acaaagaatt tgaactgatt gcgaaggact ttcaaatacg tgcagctaaa acaggtatgc
      781 tagcagattc aaagttgatt gagacagttg taaaaaatta ccaaaaatat gattttggac
      841 cattagtcgt ggatcccgta atcgtaacga aacatggaaa tcttttacta gaagaatctg
      901 ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga agtactgaca cctaattttt
      961 atgaagcaga aaagctgact ggacggacat tagcagctga agaagatttt gtgcaagcag
     1021 caaaagaact gcaagatatg ggtgcaaaaa acgttatgat caaaggacat catgctaaaa
     1081 atgcgcagca agaaacagtc aatgactacg tattgttcga atcaggaaaa tcattttggt
     1141 taaatgcacc attttatcct accgaccgga tcaacggaac tggcgattcg ctatctgcat
     1201 gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca agcgatccga ttggcaaaag
     1261 actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg gcacaaatat ggccccatca
     1321 atcattgggc agctggtgaa ttagagtaaa agaaagaagt tgtgaaaaag ttgccctgca
     1381 tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt ggttggactt
     1441 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgtcc
     1501 agctccgagt cacaaggaac aagttcaaaa aacagcttct catgtttgtt gaatattgtg
     1561 acttgtgacc gaggagttgg caggagaaca ccgtttgttc ggaagaaggg actgtgacaa
     1621 gatttttgtc acagtccctt taatcttagt atatttttct ctacttgaat aaaaaaatta
     1681 taggattgga gaaaataatc gagaaatttg ttgcttgaat aatagccagt tagacatttc
     1741 cttgactgtt tcagttgtca tggatgaaca atgtgtctca tcttctttga actgagtagc
     1801 aagttcttct aaaaactcag gatcgtaaat gacagcactt gtttcaaaat tcaatcggta
     1861 gctacgaata tcttgatttg ctgaacctac catgcagatt tcatcatcca ttatcaatgt
     1921 ttttgcatgg aggaagccgt tctcatagac aaggatttgt acattttcct tgatcagctg
     1981 ccgagcataa tattgtgttg ctcgataaat aaaaggatga tccggtttgt tgggaatcat
     2041 gattttcaca tctacaccag aggcagttgc gatttttaaa gcagcaatga cactttcatc
     2101 aggaacaagg taaggtgtct gtatccaaac acgtttttta gcagaagtaa tcaatttgat
     2161 aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg cttgaaacaa gctggatgga
     2221 tgtatcagca agcttagatt catctcgatc tgctttttta aagaaataat tcaaatgata
     2281 gccgaccttt ttttcttcag gtaccgagac gttccagtct gttaagaagc gaagctggag
     2341 caaagaagaa gcggcaccga aaatccgtat atgcgtatcg cgccaatagc cgaatttttt
     2401 tgttgtattt acatattgat tggcaatatt gaaaccaccg gtataactaa tctttccatc
     2461 gatcacaacg atttttcggt gatcacggta attcaatcgg aaacgaagta atgccctttg
     2521 tgaggtaaca aatgtatgga catgtccacc atttttgatt agacgattga aatcttttgc
     2581 ttttgtgcca tgagagccaa atgcatcata taatattcga acttccacgc cttctgcggc
     2641 tttttcttct aaaacatgta agacttgctg gccgatatta tctgtcacaa atgcgtaata
     2701 ttcgatatgg atcgaatgtt tggctttttt tagatcttga agcagtgaat ccaatttctc
     2761 ttgtccgtct gtgagaagag tgacagaatt cattcttgtc agcggcatac gatttaatga
     2821 agagaagaag tcaacaaatt gctgtttgtc tttatcgccc atgtcaggat cgtaatgttc
     2881 aatggtatct cctctaaaag actgaaagtt ttctaattct ttcaagtcac tttgtcggag
     2941 atagaatttt ttcttatccg ttaatccacg tccgaaaaat aaatataata caaagcccac
     3001 cccaggaagg gcaaacaata ctaagagcca tgcccaaatg gctgctacat ctcggggttt
     3061 gatcaaaata gccaccaaag caataagtgc atttaataga taaagggcgg ttataatact
     3121 agataccaca aaaatgttcc tccttacata caataacatt agtatagcat gatagaagaa
     3181 agaagcctac caaggtgaat atacttttca ctcctttcat gaaaatgtag gtaaatcggg
     3241 ctaaagtagt tgctttttgt aggttaataa ggtaggatat tagtacagtt gaatatctcc
     3301 ttaggggagt aaccggcaac gtttgttgcg ataatatcaa cagtaagtta accaaatggt
     3361 tttctggtat tgtatgaaat ggtgagacct aagacaaatg cgtatttgtc ttaggtctct
     3421 tttttttcag acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa
     3481 ggaacaagca caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca
     3541 gagctcaacc gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt
     3601 gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa
     3661 agatgcaatg gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta
     3721 catcgaattc ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca
     3781 aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg
     3841 agtgattcgt gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat
     3901 cgtgttgtta gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc
     3961 attaaaagta gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac
     4021 ggatatcaca agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat
     4081 cgtaacttac ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg
     4141 acaagttgct ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct
     4201 ggataaattc agtaaaaaac taagtttggc tattttggca ttgtctttat tgattttagg
     4261 tatccaactg tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt
     4321 cagcgcagtg atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc
     4381 tatcgtaaca atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat
     4441 tcggaagctt ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac
     4501 tggtacattg acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac
     4561 tggggatttc acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat
     4621 cagtgtttta gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac
     4681 ggaagtagcg tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa
     4741 caactaccca agacaagcag aacttccttt tgactcagat cgaaaattaa tgtctactgg
     4801 acacacaatt gataacaaaa attacttgtt tgttaaaggc ggaccagata tcgtcttcca
     4861 acgcagtaaa aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa
     4921 aaaattccaa gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc
     4981 ttaccgtcca cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt
     5041 tggtttgctt gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc
     5101 aagaagtgca ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc
     5161 tatcgcaaaa caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact
     5221 agatgaattg acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg
     5281 tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc
     5341 agctatgaca ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat
     5401 tgctatggga acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga
     5461 taactttgct tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa
     5521 gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc
     5581 cttagttgct gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt
     5641 caatgattca ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa
     5701 ccgcccgcca agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac
     5761 gtatcgtggt gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca
     5821 ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca
     5881 aacattccca gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag
     5941 aagtgtctta ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg
     6001 actgaaagaa gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat
     6061 gggaatcgca cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt
     6121 acaaggaaat aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca
     6181 caaggtgcaa tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga
     6241 agagttggca ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat
     6301 actgagggtc atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg
     6361 gtcacagtcc ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa
     6421 aagagataga atagataaaa gaggagaatg atttattccg aaaagtgaaa acatataaca
     6481 aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta
     6541 tgaaatccag caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac
     6601 ggagaaagtc ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct
     6661 ttccttagca aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga
     6721 aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc
     6781 gttagaagaa tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc
     6841 agcattggta catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt
     6901 gataatcaga gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg
     6961 aatgatagaa gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt
     7021 aattgatgaa ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat
     7081 tactgaagaa ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag
     7141 gtacattcgc aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta
     7201 tagactgctc ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct
     7261 ttttatgatt ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa
     7321 aaggtacatc tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa
     7381 tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt
     7441 gtgcgaagag atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg
     7501 caccagaacc gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt
     7561 ccagtagaga agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc
     7621 tgtaacggat gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt
     7681 ggtctaatac gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa
     7741 tcgcctgata atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt
     7801 ggaatgattc gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat
     7861 aatctggacg ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg
     7921 ataaaatacg cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt
     7981 caccaccgct tgccaagctg taacgagcaa tatcacttgc tatgactagt acttttcgtt
     8041 ctggatgatt tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag
     8101 cctcttttac ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta
     8161 ccgcactcgc ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta
     8221 gctcacggtc tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt
     8281 cttcatttgc acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag
     8341 gatcatcccc acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa
     8401 aggaaagacg atcaatccct attttcattt caacacctct ttacttatga taaaaaactt
     8461 actttactaa ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat
     8521 tgttaaaatt taatcttaga aaaaaataac tggaacaaga gctatttgtt tttacagcaa
     8581 agaatggtat atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg
     8641 caagaacgcc gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag
     8701 gcacacttgt gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc
     8761 aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg
     8821 ctttgaacag cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat
     8881 ctggtatgaa agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg
     8941 tgatagccgg cggaacagag agtatgtctc aagcacctat gctgaaacag tatcagtcag
     9001 aaacaaatga atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat
     9061 tttcaaatgc acatatggga ttaaccgcag agaatgttgc aacacaattt tctgtgagca
     9121 gagaagagca ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag
     9181 ccggtgtatt ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg
     9241 aggatgaagc agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat
     9301 tctcagaaga aggaactgta acagcaggaa atgcttcccc gttgaatgac ggtgcctctg
     9361 tggtgatcct tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca
     9421 tcaaagaagt agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag
     9481 ccattcaaaa attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga
     9541 tcaatgaagc atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag
     9601 aaaaggtcaa tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg
     9661 cacgtatctt gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg
     9721 cttcattatg tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc
     9781 aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca
     9841 gtcaattaat agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa
     9901 cactttctga agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc
     9961 caatggggat cgcgcagaat tttcaaatca atggcaagaa aaaatggatt ccgatggcaa
    10021 cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct
    10081 gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt
    10141 atcaagcagt gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg
    10201 aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat
    10261 tcatggggag tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa
    10321 tgggcgctaa catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt
    10381 tccctgaaga agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt
    10441 ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa
    10501 ttgccaaaaa aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa
    10561 cgcacaacaa agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata
    10621 cacgtgcggt tagcgcttcg atccacgcct acgcggcaag aaatggatta taccaaggat
    10681 taacagactg gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg
    10741 ttgccacagt aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt
    10801 tagatattga ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa
    10861 acctggctgc tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc
    10921 aagctcgttc attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga
    10981 aaaaattacg tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa
    11041 tccgggaaaa ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt
    11101 atttactgca gatctttttt tggctcttcg tcaaattgtg ttggcgaaga caaatttgaa
    11161 taaaacttag tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag
    11221 atcagacctt gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac
    11281 gcaattcgtt taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg
    11341 ggcaccttaa aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt
    11401 ttaaacgact gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta
//
LOCUS       DASJRQ010000088        11238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJRQ010000088 DASJRQ010000000
VERSION     DASJRQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.089"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            430..726
                     /locus_tag="U4A80_002174"
                     /pseudo
     CDS             430..726
                     /locus_tag="U4A80_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            784..2826
                     /locus_tag="U4A80_002175"
     CDS             784..2826
                     /locus_tag="U4A80_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1975788.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIAADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAE
                     IKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2935..4086)
                     /locus_tag="U4A80_002176"
     CDS             complement(2935..4086)
                     /locus_tag="U4A80_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1975789.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4240..4770)
                     /locus_tag="U4A80_002177"
     CDS             complement(4240..4770)
                     /locus_tag="U4A80_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1975790.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4869..5219)
                     /locus_tag="U4A80_002178"
     CDS             complement(4869..5219)
                     /locus_tag="U4A80_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1975791.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="U4A80_002179"
     CDS             complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="U4A80_002179"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1975792.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="U4A80_002180"
     CDS             complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="U4A80_002180"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1975793.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7622..8284)
                     /locus_tag="U4A80_002181"
     CDS             complement(7622..8284)
                     /locus_tag="U4A80_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1975794.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8468..10114
                     /locus_tag="U4A80_002182"
     CDS             8468..10114
                     /locus_tag="U4A80_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1975795.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10105..11187
                     /gene="uxuA"
                     /locus_tag="U4A80_002183"
     CDS             10105..11187
                     /gene="uxuA"
                     /locus_tag="U4A80_002183"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1975796.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         3767 a         2107 c         2128 g         3236 t
ORIGIN      
        1 agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct
       61 atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac
      121 ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt
      181 aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac
      241 gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat
      301 ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg
      361 tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ttttgggtcc ttatttgact
      421 ttgtccatga tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga
      481 gagcaagtga tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa
      541 ggagacttcg ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg
      601 aaagacgtaa cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc
      661 caaacgaacc ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata
      721 tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa
      781 aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca
      841 gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc
      901 caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga
      961 gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct
     1021 gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt
     1081 cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa
     1141 gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac
     1201 agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa
     1261 ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga
     1321 aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa
     1381 cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta
     1441 acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc
     1501 ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa
     1561 gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga
     1621 gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt
     1681 cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta
     1741 agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat
     1801 ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt tgacagtcta
     1861 ggaggaaaag ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt
     1921 gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac
     1981 aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct
     2041 cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat
     2101 ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct
     2161 tatcaagcaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc
     2221 tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt
     2281 acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca
     2341 gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat
     2401 ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc
     2461 aacaatggca cacttgtcta tcctaaattg attgctgcag ataaagagac aaaagataag
     2521 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg
     2581 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2641 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2701 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     2761 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     2821 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     2881 ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac
     2941 ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa
     3001 aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat
     3061 ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga
     3121 actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa
     3181 aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc
     3241 aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc
     3301 tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa
     3361 agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat
     3421 aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag
     3481 gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat
     3541 caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa
     3601 acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc
     3661 ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt
     3721 tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc
     3781 tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg
     3841 tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa
     3901 gatcacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc
     3961 agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct
     4021 ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa
     4081 ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat
     4141 tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt
     4201 gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct
     4261 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
     4321 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
     4381 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
     4441 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
     4501 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
     4561 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
     4621 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
     4681 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
     4741 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
     4801 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
     4861 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
     4921 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
     4981 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
     5041 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
     5101 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
     5161 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
     5221 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     5281 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     5341 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     5401 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     5461 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     5521 actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt
     5581 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     5641 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     5701 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     5761 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     5821 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     5881 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     5941 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     6001 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     6061 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     6121 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     6181 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     6241 gcactactag ctgctggtcc accgccaatc actaatacat cataaggttc tttttcaaac
     6301 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     6361 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     6421 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     6481 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     6541 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     6601 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     6661 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     6721 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     6781 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     6841 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     6901 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     6961 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     7021 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     7081 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     7141 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     7201 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     7261 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     7321 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     7381 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     7441 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     7501 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     7561 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     7621 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     7681 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     7741 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     7801 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     7861 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     7921 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     7981 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     8041 ggacaaagcg agcattttct gcagaaacaa gattaatttt tgaaacatac gtaccacttt
     8101 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     8161 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta
     8221 agtcagcata gataatttgt ttacgaatcg cttgataagc ttgagattgc aagctgcttt
     8281 tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa
     8341 acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca
     8401 tagcgatttt tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag
     8461 gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga
     8521 agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt
     8581 ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg
     8641 tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga
     8701 aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag
     8761 cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc
     8821 agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt
     8881 agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat
     8941 aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat
     9001 tcttgctgcg ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc
     9061 tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa
     9121 agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga
     9181 aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt
     9241 tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg
     9301 aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca
     9361 ttttcccaat ggacgtccga aattcgaaaa agctggagta ttcttgactg atcgtgcaac
     9421 ggtagataaa gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt
     9481 agctattatg ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga
     9541 tttatcgctg ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc
     9601 aggtatcatt gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc
     9661 aaatattcct gatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg
     9721 ttatggtgag acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt
     9781 attcatacca ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg
     9841 aaaaccattc atcccgagtc cagacccact attgacagac tttcagcata agctttccga
     9901 tattcaactg ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc
     9961 aaataaagaa atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga
    10021 aatgttcatc aagatggtag caggaccgaa tgccgtacga aaaacattac aggattactt
    10081 acaagaacat ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa
    10141 gaaaagatta ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca
    10201 ttgatggata taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa
    10261 gaaattgagt ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc
    10321 attaaaattg gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga
    10381 aacttggcag aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg
    10441 gtgaaatctg accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct
    10501 gatgatattc cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa
    10561 tttgaattgc cgggatggga acctgagcgc ttagctcata tcaaatcctt gcttgaagcc
    10621 tatgcatcag tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc
    10681 atcccaacat gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac
    10741 tcgatttttg gattacctcg aatcgtaaag aatcgagaag atttagattg gttgtgccgc
    10801 atagtagaca gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca
    10861 gaaaatgatg tatatgggat tttagcagaa ttttgtcaaa gagatcgaat cccatttgca
    10921 catgttcgaa atattaaatt tttagctaat cgagatttct atgaggcacc gcataaatcc
    10981 gaatacggtt cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac
    11041 ggatacatcc gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac
    11101 ggcttatatg atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa
    11161 aaaggaaaca aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag
    11221 aaggatgttc aaattctt
//
LOCUS       DASJRQ010000089        11197 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJRQ010000089 DASJRQ010000000
VERSION     DASJRQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.090"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..906)
                     /locus_tag="U4A80_002184"
     CDS             complement(13..906)
                     /locus_tag="U4A80_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975797.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4A80_002185"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="U4A80_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1975798.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="U4A80_002186"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="U4A80_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1975799.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4A80_002187"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="U4A80_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1975800.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4A80_002188"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="U4A80_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1975801.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="U4A80_002189"
     CDS             complement(6343..7062)
                     /locus_tag="U4A80_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1975802.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="U4A80_002190"
     CDS             complement(7059..8372)
                     /locus_tag="U4A80_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1975803.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="U4A80_002191"
     CDS             complement(8453..8773)
                     /locus_tag="U4A80_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975804.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="U4A80_002192"
     CDS             complement(8881..9168)
                     /locus_tag="U4A80_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975805.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="U4A80_002193"
     CDS             9295..9939
                     /locus_tag="U4A80_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1975806.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="U4A80_002194"
     CDS             complement(9998..10705)
                     /locus_tag="U4A80_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1975807.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="U4A80_002195"
     CDS             complement(10925..11095)
                     /locus_tag="U4A80_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975808.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DASJRQ010000090        10680 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJRQ010000090 DASJRQ010000000
VERSION     DASJRQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.091"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(198..1046)
                     /locus_tag="U4A80_002196"
     CDS             complement(198..1046)
                     /locus_tag="U4A80_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1975809.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1045..2112
                     /gene="gnd"
                     /locus_tag="U4A80_002197"
     CDS             1045..2112
                     /gene="gnd"
                     /locus_tag="U4A80_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN1975810.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2114..3115
                     /locus_tag="U4A80_002198"
     CDS             2114..3115
                     /locus_tag="U4A80_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN1975811.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3112..3396
                     /locus_tag="U4A80_002199"
     CDS             3112..3396
                     /locus_tag="U4A80_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN1975812.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3429..4226
                     /locus_tag="U4A80_002200"
     CDS             3429..4226
                     /locus_tag="U4A80_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1975813.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4240..5073
                     /locus_tag="U4A80_002201"
     CDS             4240..5073
                     /locus_tag="U4A80_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975814.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5091..5948
                     /locus_tag="U4A80_002202"
     CDS             5091..5948
                     /locus_tag="U4A80_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1975815.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5970..6479
                     /locus_tag="U4A80_002203"
     CDS             5970..6479
                     /locus_tag="U4A80_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975816.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6516..6917
                     /locus_tag="U4A80_002204"
     CDS             6516..6917
                     /locus_tag="U4A80_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975817.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7029..7382
                     /locus_tag="U4A80_002205"
     CDS             7029..7382
                     /locus_tag="U4A80_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975818.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7416..7859
                     /locus_tag="U4A80_002206"
     CDS             7416..7859
                     /locus_tag="U4A80_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1975819.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7876..8961
                     /gene="uxuA"
                     /locus_tag="U4A80_002207"
     CDS             7876..8961
                     /gene="uxuA"
                     /locus_tag="U4A80_002207"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1975820.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8954..9667
                     /locus_tag="U4A80_002208"
     CDS             8954..9667
                     /locus_tag="U4A80_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1975821.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9874..10023)
                     /locus_tag="U4A80_002209"
     CDS             complement(9874..10023)
                     /locus_tag="U4A80_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975822.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10419..10619
                     /locus_tag="U4A80_002210"
     CDS             10419..10619
                     /locus_tag="U4A80_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1975823.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3563 a         1692 c         2315 g         3110 t
ORIGIN      
        1 tactaacttt gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca
       61 aagatgcgat gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag
      121 aaccaaaaaa gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg
      181 ctttttctgt tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata
      241 gaacgacgtg attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg
      301 cttagttgag aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata
      361 gaaataaaaa cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg
      421 attgtttttg ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc
      481 cctgtgtgag aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat
      541 tgaatatggt aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa
      601 gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca
      661 ttgataatag tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt
      721 aaagttgaaa tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc
      781 tgaatatttt cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt
      841 ttaaaatctt taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca
      901 gcaatgccca gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt
      961 tctaaaatat aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg
     1021 atttgtagtc ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt
     1081 ttttccgttc tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat
     1141 atttttgtaa aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga
     1201 ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta
     1261 aacttaaaag agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt
     1321 caggcaagag aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt
     1381 gagcagaaaa aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta
     1441 agaaaacttt ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat
     1501 tataaagatt caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat
     1561 tgtgggactt ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga
     1621 gataaagaaa cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga
     1681 tatctgtatg ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc
     1741 gaatatggaa tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat
     1801 gcttttgact tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg
     1861 ctgatggaaa tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga
     1921 atcgtttctg catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt
     1981 ccagttccag taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt
     2041 ttttcagcaa aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa
     2101 aagggtgagt aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag
     2161 agtctttcca caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt
     2221 tgatcaagag attccaggaa aagaactagc ggagttactt aaaggattca atatcattat
     2281 tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt
     2341 gatttcccga catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca
     2401 gacaatcgta tctatcatac ctgctttaat tgaacgaaat gctgtagcag aaaataatat
     2461 aactaatttg ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga
     2521 tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt
     2581 gattggtgca ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg
     2641 tgatgttcta gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa
     2701 aaaagtagaa cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt
     2761 gacagaagag aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt
     2821 ttatctatct aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct
     2881 gaaaagcgga aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca
     2941 agatcatccg tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac
     3001 aagggaatgt ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca
     3061 gcaccttctt cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag
     3121 aaaatggtga acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt
     3181 ggtatttcaa aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc
     3241 cacggaacgg tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg
     3301 attcaatttt tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata
     3361 gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga
     3421 ccgatctagt gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt
     3481 tagctagtaa ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag
     3541 cagaagggaa aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat
     3601 tagtgattga ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat
     3661 ggtatgaatc tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc
     3721 tagcacaaga tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa
     3781 tccaagatat ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct
     3841 ttgctgttgc tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac
     3901 atgctatgca tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag
     3961 aaaaagtagc gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag
     4021 acgagttata tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat
     4081 tatttccgaa agataaacag ataatagata aaatggctga atttattgat tcgaaagaga
     4141 aagtgcattt gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac
     4201 tggaaggtta tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta
     4261 caaagtttgt tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga
     4321 gccacgttga ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg
     4381 ggggaagttg cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt
     4441 ttttctccag gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca
     4501 gtcgcattat taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga
     4561 ttgctcggaa gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat
     4621 acagacaaag ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat
     4681 ccaacggttg cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg
     4741 gcgccattga tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt
     4801 gaagtagtag gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt
     4861 cgaaaagatt atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct
     4921 ttagatatta cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc
     4981 cagtcgcaaa aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt
     5041 gatgacgaag atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa
     5101 taatggataa tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct
     5161 atctgagatg gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat
     5221 cactactata cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa
     5281 aagcagcata taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg
     5341 tgattactgg cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa
     5401 aagagatctt gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac
     5461 tagcaggaat cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg
     5521 cggtaccttg ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg
     5581 ggttgtatca ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta
     5641 atgctacagg cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt
     5701 tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga
     5761 aatttgcgat atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag
     5821 ggatattacc tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga
     5881 aagtcacaca agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta
     5941 ttttataaaa agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg
     6001 agcgtttgat ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca
     6061 tttttgttgt agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg
     6121 caggaagccg tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt
     6181 gggatgaata tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga
     6241 tttacgactt agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc
     6301 aaaaaaatcc tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg
     6361 ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca
     6421 ctccatctgc acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa
     6481 caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg
     6541 attgggaatt tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc
     6601 gttttctttc agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg
     6661 cttacgatac gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag
     6721 agaaagttat ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt
     6781 ttgattggaa agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt
     6841 tgttcagttt aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg
     6901 atctgctatc cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat
     6961 tgggaaaagc agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca
     7021 tattaacgat gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg
     7081 ttgatgaagg ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag
     7141 aggctattct tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa
     7201 attatttttc aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg
     7261 tcaattatat ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag
     7321 atttacgagg gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat
     7381 aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac
     7441 atggtaagtt gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc
     7501 atattgaaac aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca
     7561 aagaaaaatt aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta
     7621 gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag
     7681 cagccactta tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc
     7741 agatgcttca cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata
     7801 atttaactgt ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga
     7861 aggaaggctg aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc
     7921 ccattaaaat atgttcgcca aataccggga ataaatggga taattggaac tttgctgact
     7981 aaaatgcctg gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag
     8041 agccaaggac tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca
     8101 gggacagatg aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct
     8161 cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact
     8221 gatttgttta atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc
     8281 gatcagatgg agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa
     8341 ttgccgggtt gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa
     8401 ggaatgaccg aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca
     8461 gtgtgcgaaa aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt
     8521 tttggttttc ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta
     8581 gattctccat ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac
     8641 gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac
     8701 gtggagtttt taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc
     8761 cttgatatgt atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc
     8821 cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat
     8881 cgtgcaatgg gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca
     8941 ctgggagagc attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt
     9001 agaaactgga cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga
     9061 aatgcgtaag gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca
     9121 aatgggcatt ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca
     9181 atataaagaa aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga
     9241 aattttatct tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg
     9301 ggtaaaagta gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat
     9361 ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc
     9421 aaaaatgaat attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga
     9481 agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc
     9541 cgatcataat gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc
     9601 agactatgaa ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta
     9661 tctctaataa agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg
     9721 aacagatgaa aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa
     9781 tttagaacaa acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag
     9841 aacgagaatc gtccatatga gggtttccac tccttatgca gattttttta attcttgcac
     9901 tgcttgttcg atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac
     9961 aaatagattc aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt
    10021 catagccatt ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa
    10081 attccattta taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc
    10141 aaaagttgca gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt
    10201 caactttttc ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta
    10261 tccttgtgtt attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat
    10321 ttgagcgata tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga
    10381 aaaaacgtac attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat
    10441 ggttcaacgc agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg
    10501 cacatttttc agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt
    10561 cttacgatgt tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat
    10621 aaaaattgac ttttaaccgt cttttcgaaa gaaaagacgg tttttttgta cgaaaaaatt
//
LOCUS       DASJRQ010000091        10648 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJRQ010000091 DASJRQ010000000
VERSION     DASJRQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.092"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..646
                     /locus_tag="U4A80_002211"
     CDS             23..646
                     /locus_tag="U4A80_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975824.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="U4A80_002212"
     CDS             1096..2145
                     /locus_tag="U4A80_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1975825.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="U4A80_002213"
     CDS             2142..3023
                     /locus_tag="U4A80_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975826.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="U4A80_002214"
     CDS             3026..3796
                     /locus_tag="U4A80_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1975827.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="U4A80_002215"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="U4A80_002215"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1975828.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="U4A80_002216"
     CDS             5107..6270
                     /locus_tag="U4A80_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1975829.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="U4A80_002217"
     CDS             6387..6797
                     /locus_tag="U4A80_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1975830.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="U4A80_002218"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="U4A80_002218"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1975831.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="U4A80_002219"
     CDS             9370..9654
                     /locus_tag="U4A80_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975832.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="U4A80_002220"
     CDS             9814..10050
                     /locus_tag="U4A80_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1975833.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="U4A80_002221"
     CDS             10047..10445
                     /locus_tag="U4A80_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1975834.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3716 a         1773 c         2253 g         2906 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatat
//
LOCUS       DASJRQ010000092        10529 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJRQ010000092 DASJRQ010000000
VERSION     DASJRQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.093"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..526
                     /locus_tag="U4A80_002222"
                     /pseudo
     CDS             6..526
                     /locus_tag="U4A80_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(428..1144)
                     /locus_tag="U4A80_002223"
     CDS             complement(428..1144)
                     /locus_tag="U4A80_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEN1975835.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1194..1403)
                     /locus_tag="U4A80_002224"
     CDS             complement(1194..1403)
                     /locus_tag="U4A80_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975836.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            1680..2953
                     /locus_tag="U4A80_002225"
                     /pseudo
     CDS             1680..2953
                     /locus_tag="U4A80_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3060..4091)
                     /locus_tag="U4A80_002226"
     CDS             complement(3060..4091)
                     /locus_tag="U4A80_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975837.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4098..4928)
                     /locus_tag="U4A80_002227"
     CDS             complement(4098..4928)
                     /locus_tag="U4A80_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975838.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4925..5740)
                     /locus_tag="U4A80_002228"
     CDS             complement(4925..5740)
                     /locus_tag="U4A80_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1975839.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5737..6561)
                     /locus_tag="U4A80_002229"
     CDS             complement(5737..6561)
                     /locus_tag="U4A80_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEN1975840.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6551..7651)
                     /locus_tag="U4A80_002230"
     CDS             complement(6551..7651)
                     /locus_tag="U4A80_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1975841.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7829..8614
                     /locus_tag="U4A80_002231"
     CDS             7829..8614
                     /locus_tag="U4A80_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEN1975842.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8647..9030
                     /locus_tag="U4A80_002232"
     CDS             8647..9030
                     /locus_tag="U4A80_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1975843.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9106..9237
                     /locus_tag="U4A80_002233"
     CDS             9106..9237
                     /locus_tag="U4A80_002233"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1975844.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9206..9442
                     /locus_tag="U4A80_002234"
     CDS             9206..9442
                     /locus_tag="U4A80_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1975845.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9520..10491)
                     /locus_tag="U4A80_002235"
     CDS             complement(9520..10491)
                     /locus_tag="U4A80_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1975846.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3105 a         2467 c         2044 g         2913 t
ORIGIN      
        1 gctctatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
       61 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggga
      121 gattggatga ctcaccttcc cattgtgaac actgtggatc gattcgtatt atccgcaacg
      181 gttcctatac gacacgtact cagatgttga aagtcaaaga aaagttgact attttagaat
      241 taaaacgtac ccgttttcta tgctatgatt gtgggcagac cttttctgcg aaaacggact
      301 tagttgatga acatcatcaa ctaacaaagg aactgaaaca ggcgattctc atagaactgt
      361 acgaaaatca atcacgtaat ctgaaaatca gaagaagtac cgtcggctca agcgatattg
      421 gaaactctta ctcaaagatt ccacgacatt ggagccgctc aaacggcatt atcatcgact
      481 gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa ccgttgtgca caatggtgtc
      541 ctgaacgtac agcgacacct tccatatcca gagcagtcgc cacatcacgg ggatggatgc
      601 catccaaatt aaaagcaacc acgccagtat gatgttcagg atcttgcggt ccgtaaatgg
      661 tgataccgcc gatcgcttgt aatttaggta aaacgtaggc aaccagttct tgctcataac
      721 gatgaatatt ctccatacca atctcattca agtaatcgat tgctcctgca atgttcggag
      781 taccagcttc aaatttccat ggcagctctt tccatgaact atcgtaaaga ttgacaaaat
      841 ctatcatttc accgccgaat tcgactggct ccatttgttc taacaattga cgtttgccat
      901 ataatacacc gattcctgtc ggcccgcaca ttttatgccc gctaaaggcg aaaaaatctg
      961 catccaacgc ttgtacatca actgtcatat gcggtgctgc ttgggcaccg actggactat
     1021 cctcaactac aacagcctgt tcaaccctca gccgtttcaa gcacaacaaa taaccttctg
     1081 gatctggttt gccgacctga agatcatcga tcgtcacaaa ggaatcaatt ttttccttaa
     1141 tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag ttcagaattg agactactcc
     1201 gattttccag tgctgctgat ccgctgctca aaatgttcag catccactgg tctcccaacc
     1261 gcttgactta tcccttcata aaaggacagt tctgctgccg tcccaacacc ttgttgatag
     1321 tctgcaaaag gaattttata gccgaactgc tccagtaaat aggcttcagt aaatagtgat
     1381 gaatactctc gtaaaacatc cattcactgt caatcatcgt cccatcaaaa tcaaaaataa
     1441 gtgctttggt catcttcttt cccccttacc caaaacaagc aaaagctgtg tcctgctctc
     1501 tctacagtat cagcataaaa actgccgtct gaaaagtcaa aggtgaaaga aatgtggcgc
     1561 tataatttct aggactgata gtaaatcatt ctatcagtcc ttttgagcat aaaaaaacaa
     1621 aacatacaga agctccgtta gaattgaatc accacaacac aatcaaagga gcgttctgta
     1681 tgcctgatct tcatcttatc aaactattac taggaatcaa agatggaaac attcaaatca
     1741 ctcatgtcga gaagaaaaaa atacattcaa tcaacagtat cgttgtttct gccacgctca
     1801 ttaaaacgat tcagccctgt cctcagtgta aacaggtgaa tcaacatggg ctgatcgtta
     1861 agaacggaaa gaaaaaatcg ctggtccttc tcaataagtg tgcgaatcaa cggacttatc
     1921 ttgctttggc aaagcaacgg tacctctgtc gtggctgtca cacctatttt acggcgaata
     1981 cctacattgt tgaaaaaaac tgttttatcg ccaaacaagt acattataaa atattggagg
     2041 aattgactga aaaacaagca atgacgacga tttctaaaca ttgcggcgtt tcttggtcaa
     2101 ccgtttcgcg aacactcgct tctctgctcc ccatgacaaa cgtgaaaata aattggttgc
     2161 ctcgctgttc attggtagat gaatttcgtt cattgaaaaa tcaagtcggt ccctattctt
     2221 tttcttgtat ggatggcgat actgggaaat tactggatat tcttccttca cgtaagaaga
     2281 aagatctcat ttcttatttt atgcaatttg aacgacgtgc acgtttaaac gtaaaaatac
     2341 tcgtaacaga tatgaacgcc tcttatgctt tgctgataaa agagtgcttt cctcatgcca
     2401 aactcgttgt ggatcgcttt cacatcgtca agcacctcat tcgcagtttt aaggatattt
     2461 gactatgagt catgaaaagc ttcgaccgaa acgatccaat acaagccaaa cactatcgac
     2521 aagtgaaggc tttatctcga cttctgatca aacgacagga cacgcttgtt tatgataaat
     2581 ggacaaaatg gcgtaatttt ggttgggcct atttaaccga aagtgaagtc gtagaacgtc
     2641 tactttctac ttctgatgaa ctacgaatag cttatgctta ctatcaagaa attcttcaag
     2701 ctttttatga caaagaagcg gacatcttct ttcagcttgt aaaaacaatg cccaagtcgg
     2761 ttccaagaga actgcatcat ataaaaaaag cgtttataaa ctatgaatcc ggcattcgtt
     2821 tagccttaga gttgccttac tcaaatggca aaatagaaaa tctgcatacc cacatcaaag
     2881 cattgaaacg aatagcttac gggttaggat gagaaccaga attttcttaa tcaatcatct
     2941 gatcacttat taaacataaa aaaacatcta aagcagaaaa tcaattctac cttagatgtt
     3001 cttatatcac tctatcagtc ctatttgaca aagagccaga aatgtttgcc gactatacac
     3061 tagctttgtt gtgcaaaatg ctgagtatca atttccacag ggattgctag aatatctttc
     3121 agctgaatgt ataaggcatc gctaattttc tcttcccgat cgttcaaccg atccactaaa
     3181 atttctttgc cctccagcgt ttgaaaaaca taccgttgaa tgttgcctaa aataaatgat
     3241 tccttccata gaaccggaat cttaaccccc tgaggaaccg gttcaaattg aatcagctcc
     3301 ggtcttaaat aataaatgca gcgattgccc ttagcggaaa actcgtgggt atactgattc
     3361 agctcatacc attcgaaacg attatagttg ccgataaatt ccgcaacaaa tcggctctga
     3421 ggattctgat acaattcctt cgaggtggaa acctgagcca cccgcccagc ttccatcaca
     3481 aaaatccgat cactgattcg catcgcctct tcttggtcat gagtcacaaa aatcatcgtc
     3541 atccctagcg attgctggat agcgcgcaaa tcccgctgca gctgttttct aattcgggca
     3601 tccaaagcac ttaatggctc atccagcagc aggactttcg gttcagtgac caatgagcga
     3661 gccagtgcca cccgttgtcg ttgaccgcca gaaagaaact ggacattttt atctgcctgc
     3721 tccgtcatat ccaccaatga tagcatctca aaaacctttt cctgaatcac ctcaggactc
     3781 agcttcttta ccttcaaacc aaaggctaca ttttcaaaaa cagtcatagt tggaaacaat
     3841 gcatactgtt gaaaaacaaa gccgatattg cgatccttcg taggcttatc ggcaatattc
     3901 tcattgtcca gcacaatctc accgctcttt ggttgttcca atcccgtgat acagcgcagc
     3961 aacgtagact ttccgcaccc gctgggacct agtaaagtaa ccagctcacc cttttcaatc
     4021 tgaatttcat caatagctaa aatctgtttt tcatcaaaaa acagattgag atcatgtatt
     4081 tctaaaaaca ttttccgtca caactccttt aagggcttct ttttcgactg cttctcttcc
     4141 ctcgtctgct taagggacag ctgttgcttt tgtcgataag tcagcataaa ggtagttaaa
     4201 gcaattccca ccataaacag caaatacaaa accatcacag cgctagccaa atggccgttt
     4261 tcgttcatcc gccgcagcat atagatgcgt accgtctgaa atcgaccgcc aatcaacagg
     4321 ttcgtcagca tgtactcccc aaaaaccgaa gaaaaaatca tcagagaaac tagggtcaca
     4381 ccgatacgga tgtttggaaa cagcacctgg ataataatcg tagacatcgg ggcgcccaag
     4441 gtttctgctg catccactaa ttccttcaga ttaattaagc gcatctggtt gttaagacta
     4501 agatagacaa tcggcaacgc cgtaataaac aatgcaccta ccaacactac aaacattggg
     4561 ataccagttt cagcatacgt tcgaagcaaa gccgttacca aaatcactcc tggtaaagca
     4621 tacggcagca ggagcagctt ttccagccaa cgattcaaac gagggaaata caaatgaatc
     4681 caaataatgg tgggaatcat caacaacaga atcgtcacca agaccacacc tgctaataaa
     4741 agtgagcgac ccaccgcagc aataaattcc ctgtccgtca gcagctgttg gaaccattgc
     4801 aaggtaaaat cactaggtaa aagcgatttt gaccatttag tggagctggc atacaaaatc
     4861 gttccaataa tcggcagcaa gtaatacagg acaaagcaga acaaaatcgg ttttattaac
     4921 tttttcacgt ctgcaccacc ttgcctttct tttgaataat caacattgcc gttaccagca
     4981 ggacacctaa agcgactgcc aacgcactgc ctaaattagg cttggcgaac acatctcccg
     5041 acactaaagc cgcaattcga atcgttaaaa gatttacatt actgctggtc agggcatacg
     5101 ctgtttcata ggtccccatg ccattagcaa ataagatgac aaaggtgctg gcaatcgatg
     5161 gtcgcaaaaa aggcaaagcc accttgaacc aagcatacgc tgatgaagcg cccagcaatt
     5221 gtgcggactc cagccattcc tttttcactt ccttcatgct gggatacaaa aacaagaccc
     5281 ctaacggaat ttggaaatag gtatacgtga tggtcaatcc ccaccaagaa tacaaattga
     5341 agtccgctag aaatggcaaa atcattttca gcacacctac attccccaac aaaataataa
     5401 aagagaatgc cagcggaatc ccagcaaaat tagctgccaa attggaaaca aaggtgattt
     5461 tctcctgcaa tttagtcgat agattcatta agcataaaca taaaataaac gtcccgatta
     5521 acccaatcac actggagctg acagcaatca gtacgctgtt aacaaaggcc tgtgtgtaat
     5581 atttgttgct gaaaatatca ctgtaattgg ccaatgtcca ctggttagtt aaatcactgt
     5641 gcaagctgca cagaaccata aaaagcagcg gcagcagtaa aaatgcacag aagataaagg
     5701 aggcaggaac cagaaccaag cgttttttca tctctttcat cccctcatca attcttgaat
     5761 gtcctgcatt ttctctgacg gtgcaattcc taataaccag caaaacaatg gagccgccag
     5821 cagctgtggt acagtttgtt ccttcagctt ttggcgttta tttgaaatca aataaaaagg
     5881 aacttcacga tgtgcagcca agctgccgcc atgcagtccg tgcgcatcca tcccgtgatc
     5941 cgcagtcacc acgacttgat aacctttttg cagccattca ggaaggtaaa tagccaataa
     6001 tctgtccgcc tgattaaccg ctgctgcata ttcatgagaa tcggcggtaa atttgtgacc
     6061 gacatcatca atattcatcg aatgtacgac caaaaagtct ggctgatact gattcactaa
     6121 ataatgggca tccgcaaata aatgcgaatc agggtaagtg tcctcactat aaaagatgcc
     6181 atgctgaatc agtcgctcct cctgatgctg gatgcgatca accaaacgat tatagggggc
     6241 ttgattgtac aactcgctgt accagtggta agcggcggct cctgtttttc cgccagcttc
     6301 cttaactaat tcaaacagcg accactcttt ggatcgctga gcatggtaat tactatagat
     6361 gccattttca taggctggaa cgccggtcat cagtacttcg tataacggac gtgaattgct
     6421 gggcagctca ctttttacct tcaataagct tgcttgctgg tgctcaacta aatgattcat
     6481 gaacccaagc tgggcgacgg ctgtgtcgta gcgacagccg tctagtataa ctacaatcag
     6541 tttgtcaagt ctactttgca taggtcagta cctccgcctg ccaaagctct ggaagctcta
     6601 cagaggtctt gttccaagct tcattgtctt caactggcac cgccgcttca taagcatcct
     6661 ccggcagtaa tttttcagct acttctttag gcagctctac cttgcgaata ggtcgcgcat
     6721 agccctttgc caaattgatt tgcccttcat cagataaaat atattcccgt gctagctttg
     6781 ccgcattggg atgaggggca tttttattga ttaaggttgt atacccgcta atgatggagc
     6841 cgtctgttgg cacagttacc tcaaaacgct cggagtcaat ttggtcacga taatttagtc
     6901 cattgaagtc ccaaacaact gccacatcga tttcaccttt ttcaagattt gctacgctgg
     6961 catcaacggt ggacaaacgg cctttttctg ccattttttt gtagtaattc agacctggct
     7021 gaatatcctt ttcgtcacct ccgttggcaa tcgccgcagc caatacagca aattgtgctt
     7081 ggttggcaat cgtcacatcc cccacgctaa cttgatagtc accctcttgc agctccttcc
     7141 agctagtcgg tgcttgcttc acattctctt tatcggtgat aaaggaaatg gttcccgtat
     7201 agcttaacaa ccaatcccca tcatcatcct ttgcccaatc cggaatttcc tcccaatagt
     7261 tggtcttata aggtaaggtt aactctttgc tttcagccaa cggaccgaag ctgataccga
     7321 catcaccaat atcggcggtt ccattttttc cttctgattc aaacttagcc aattcttctg
     7381 cactggacat atccgtatcc acgtgactta agccgtattc tttttcaagg tcgttccaag
     7441 tgcctaccca atttgcccaa gtatccggca ttcccaagct ggcaatctct ccttcttcct
     7501 tggctttttc tataatcgta tctaaagagt cgtctgccgc agccgattgc ttcgttgctt
     7561 cacctgagcc gcagcctgtc agcaggatac ttactgttgc tgcacaaaat aaaccgatag
     7621 tcatgttacg aattttttca ttcattttca ttcattcttc tcccctttcg ataattccat
     7681 tctaacaaag ctttgtaatg agagaatcag tttcttgaaa aggtttcatg aacaatgtaa
     7741 aggttgtata tggtttgtaa atttcgacga attcccgtta accttctaaa gcttttgcgg
     7801 tatgatagta gaaaaaagga ggctccctat gcagtattat gatcagcatc tccacacgta
     7861 tttttccttt gattcagagg aaaaatttga aaactattta gcctatcagc cggagttttt
     7921 cgtttctacg gatcactttg atttaaacaa tccatctacc ggaaaagatg acattccgga
     7981 ttatccagcc tatataaaca aactggactc tttaaaagaa acctattcca ccaaatttct
     8041 gcgaggtatc gagattggcg ttgttccagg acaagaagag cggatcgtgg attacctcgc
     8101 tcagcatcct tatgatttaa agctggtaag tattcatcaa aatggccgct ttgattatat
     8161 ggacgatgtg gttttaacca aagatcccat tcaaactacc aaggaatatt ttgatcagat
     8221 gttgcaggtc ttgacggatt ttccagaagg agatattttg actcactttg attatggatt
     8281 acgccgcttc accttcacag cggaagaatt cgcggagcat tttgaggctc agctgctaac
     8341 gattttcaaa aaggttgttg aacgagaatt ggcgatggag ttaaatgcca agagcttctt
     8401 gaaatacggc aacgaagacc tttaccgtta tgccgttcct ttgtatcaaa gtgtcggcgg
     8461 aaagctcttt accttaggct cggatgctca tgttgccgaa gattatcagc tgggctttaa
     8521 aaaaatgagt cagctgttgg cagactgtca ggtagaaaag ttgctagtca ttcaaggaaa
     8581 agaacgccgc ctgacgccgc tgccgatttt gtgattccta tataatagaa gaaagtggtg
     8641 aaaaaaatga gcaacctgtg ggactacaat caagaggcac ccatccatta tttaattgcg
     8701 cgacattggg atgcgttgaa aattgaggcg gtttgccgat ccttgttagc agccgttcca
     8761 aaacaacaac tggaaaattt tttggtggct gacagcttgc agcgggaaaa agttcaggct
     8821 tattttgcag cctttaaaga tcagcctttg gaatatttgc atgcgcagtt tcatcttttt
     8881 tatcaagtag ccgccccaga tgactacaac gatttacgag gacagctgca attgaccttt
     8941 caagcggatg aaaccgccta taccgtttta ttgggaatgg ctcgccttgg tgatcaggcg
     9001 aaggtcgagt ggcggatttt cgatatttga tggcaggatc aaggcgtttc agttgcaaac
     9061 cttcctctct ttcaattaga aaaaccgcag caggaagaag tttgtgtgct ggtcaccgat
     9121 gctggtgaaa ttcgtctccg cttatttcct gaccaagctc ccctggctgt agccaactgg
     9181 cgctccctag ctgagcaagg cgtttatgac aacacacctt tcggctcgtg tcattaagga
     9241 atttgttatt caaggtggcg ctttggatgg ttctggtgat gaagctgttt ccagctatga
     9301 gggctttttc gctgatgaag tgcatcgcgg actttatcat tttaatggcg ccttggcctt
     9361 aggggaccac ggcccccaca ccaacggcaa ccagtttttt attgtgcaaa acacaaaggc
     9421 gcaggctgac ctgctaatgt aaagcaagca tacttggagc ttggaggact accggagctg
     9481 gatggtcgtt atactgtttt tggacaggta taccaagggt tagaaatcgt agcagcgatc
     9541 gcagctcagc cgacaaacag tgaagatgta ccgattgagc cagtgttcat tcgacaaatt
     9601 aacttcgaaa aaacatcgta aaaagacact cactgacttc agcaaaagct ctattcttga
     9661 gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg gattcaataa
     9721 ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc gagccagttg
     9781 atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca acggatactc
     9841 cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta attctaacgg
     9901 tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg tcaggttcat
     9961 attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat actcaattaa
    10021 aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt gtttgtggag
    10081 cttgctggta aacattttta catccacaaa caatgtatcc acatagggag ctaccttttg
    10141 gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc caatgtattt
    10201 cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc ctccgctaat
    10261 ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct catccattac
    10321 ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt gtgattcggg
    10381 aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg tccgtaaacc
    10441 agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca tatagatttt
    10501 tgaattacac actagtttaa actaaaaca
//
LOCUS       DASJRQ010000093        10084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJRQ010000093 DASJRQ010000000
VERSION     DASJRQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.094"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..2229)
                     /locus_tag="U4A80_002236"
     CDS             complement(109..2229)
                     /locus_tag="U4A80_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1975847.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="U4A80_002237"
     CDS             2459..3637
                     /locus_tag="U4A80_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1975848.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="U4A80_002238"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="U4A80_002238"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1975849.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="U4A80_002239"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="U4A80_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1975850.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="U4A80_002240"
     CDS             complement(4990..6243)
                     /locus_tag="U4A80_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1975851.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="U4A80_002241"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="U4A80_002241"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1975852.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="U4A80_002242"
     CDS             7821..9167
                     /locus_tag="U4A80_002242"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1975853.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="U4A80_002243"
     CDS             complement(9475..9861)
                     /locus_tag="U4A80_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1975854.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10084)
                     /locus_tag="U4A80_002244"
     CDS             complement(9910..>10084)
                     /locus_tag="U4A80_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1975855.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2977 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgtt
//
LOCUS       DASJRQ010000094         9879 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJRQ010000094 DASJRQ010000000
VERSION     DASJRQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.095"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            254..733
                     /locus_tag="U4A80_002245"
     CDS             254..733
                     /locus_tag="U4A80_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975856.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="U4A80_002246"
     CDS             766..2994
                     /locus_tag="U4A80_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1975857.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="U4A80_002247"
     CDS             3123..4766
                     /locus_tag="U4A80_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1975858.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="U4A80_002248"
     CDS             complement(4839..5384)
                     /locus_tag="U4A80_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1975859.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="U4A80_002249"
     CDS             complement(5407..5892)
                     /locus_tag="U4A80_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1975860.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="U4A80_002250"
     CDS             complement(5882..6694)
                     /locus_tag="U4A80_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1975861.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="U4A80_002251"
     CDS             complement(6833..8365)
                     /locus_tag="U4A80_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1975862.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="U4A80_002252"
     CDS             complement(8474..9505)
                     /locus_tag="U4A80_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1975863.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3293 a         1836 c         1902 g         2848 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtatta
//
LOCUS       DASJRQ010000095         9764 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJRQ010000095 DASJRQ010000000
VERSION     DASJRQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.096"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            188..358
                     /locus_tag="U4A80_002253"
     CDS             188..358
                     /locus_tag="U4A80_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975864.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            372..974
                     /locus_tag="U4A80_002254"
     CDS             372..974
                     /locus_tag="U4A80_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1975865.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            990..1667
                     /locus_tag="U4A80_002255"
                     /pseudo
     CDS             990..1667
                     /locus_tag="U4A80_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1978..2142
                     /locus_tag="U4A80_002256"
                     /pseudo
     CDS             1978..2142
                     /locus_tag="U4A80_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2179..2475
                     /locus_tag="U4A80_002257"
     CDS             2179..2475
                     /locus_tag="U4A80_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEN1975866.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2476..2892
                     /locus_tag="U4A80_002258"
     CDS             2476..2892
                     /locus_tag="U4A80_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEN1975867.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            2912..4456
                     /locus_tag="U4A80_002259"
     CDS             2912..4456
                     /locus_tag="U4A80_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1975868.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4599..4823
                     /locus_tag="U4A80_002260"
     CDS             4599..4823
                     /locus_tag="U4A80_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975869.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4838..5707
                     /locus_tag="U4A80_002261"
     CDS             4838..5707
                     /locus_tag="U4A80_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1975870.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5688..6422
                     /locus_tag="U4A80_002262"
     CDS             5688..6422
                     /locus_tag="U4A80_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1975871.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6455..7363
                     /locus_tag="U4A80_002263"
     CDS             6455..7363
                     /locus_tag="U4A80_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1975872.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7360..7902
                     /gene="sat4"
                     /locus_tag="U4A80_002264"
     CDS             7360..7902
                     /gene="sat4"
                     /locus_tag="U4A80_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEN1975873.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            7995..8789
                     /locus_tag="U4A80_002265"
                     /note="aph(3')-IIIa"
     CDS             7995..8789
                     /locus_tag="U4A80_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1975874.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9065..9637
                     /locus_tag="U4A80_002266"
     CDS             9065..9637
                     /locus_tag="U4A80_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1975875.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3434 a         1531 c         2148 g         2651 t
ORIGIN      
        1 actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac
       61 acttgatctt ttaagttgct atctttttaa ggtcaataga gtatacttat ttgtcctaat
      121 atggttttag cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag
      181 gagtaaaatg aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt
      241 taatgagcaa caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca
      301 atttgaaatt gaaaaagtag atatagctga ttggttattg caaccgagag aattttagag
      361 gttggttgaa aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag
      421 atagacaact gaaagcgtta gagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc
      481 aatcggtcga acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatatag
      541 ttgttgttac tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga
      601 atcaaattca aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta
      661 ttgaagatga aaatttaaga cggctgatta ataatttagt gattgaattg tataagtacc
      721 aagcggaatc tgaacgcaaa cgaattaaag aacgccaagc ccaaggaatt gaaattgcta
      781 agaaaaaagg aaaattcaaa gggcgacaac ctaaattcaa agaaaatgat ccacgtttac
      841 aacacgcttt cgatttattt ttgaatggtt gttccgataa agaagttgaa gaacaaactg
      901 gaattaatcg ccgaacgttt agaaggtatc gatcaagata caacgtgaca gtagatcaaa
      961 gaaaaaaaca atgaaaagag ggatagttaa tgagtacggt tattttagct gaaaaaccaa
     1021 gccaggcatt agcctacgca agtgctttaa aacaaagcac caaaaaagac ggttattttg
     1081 agatcaaaga cccactattt acagatgaaa cgtttatcac ctttggtttt gggcatttag
     1141 tcgaattagc agaaccaggt cattataacg aaaagtggca gaactggaaa cttgaatctt
     1201 tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga aagcagttta
     1261 aaattgttgc tgaacttctc aaaaaggcaa atacaattat tgtcgcaaca gatagcgaca
     1321 gagaaggtga aaatatcgcc tggtcgatta tccataaagc aaatgccttt tctaaagata
     1381 aaacgtataa aagattatgg atcaatagct tagaaaaaga tgtgatccga agcggttttc
     1441 aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa acacgccaaa
     1501 ttgccgattg gttgatcggc atgaacgcaa gccctttata taccttgaat ttacaacaaa
     1561 aaggcgtaca aggtacattt tcattaggac gtgtacaaac accgacttta tatctcattt
     1621 atcaacgcca ggaagcaata gaaaacttta aaaaagaacc ttttttcctg aataattcat
     1681 agttttacta atagtctttc ctaacattga tttaatagtc tttaaagtat tctgcttcat
     1741 cacccattag gtgatgaaaa aagaacccct ttctgattta gcggacaaat actttgacct
     1801 aatctcagaa gatataaaag aaaaaataat aaaatatccg atttatactt tgtataaaga
     1861 actaaagtag ttagcaaatc atttacagca aatcactatg ttccacatac cattttacat
     1921 acttacctta tccacgacac atgttgagat agtatcgaag ataaagaaag ttgctccatg
     1981 ttgtttttgc gagatgggga aatcaaagcc acattgacaa ccatgatgaa taagcttgct
     2041 ttcagtcata agctaatcct agaaccactt ttcaagtcag ttagccaaat cgatgaagaa
     2101 agcgaccgtg aaagaatgga cgctattgat aagctaatgg agcaactatt ggaagaacgc
     2161 aataccctta ttgcactgat gtccaaaggt ttcctagaac ctgctctttt taatcaggaa
     2221 cgaaatgttt tagatagtga gattaaaaat cttactaccg aaaaaacaaa cctggtaacg
     2281 aactctgcga gtggggtttt gcgagcaaac gagataaagg acctcattaa ttatgtgtca
     2341 gcagataatt ttaatggtga ctacacggaa gaactatttg aagaatttgt agtgaacatc
     2401 attgtaaatt ccagggatga gctgacattc aatttgaaat gcggtctttc cctgaaagaa
     2461 aaggtggtga gataaatggc atatattcca tatggataca aaattcaaga tggagtggtt
     2521 actgtcgatg aaaaggcagc aggtcaagta aaggtattct ttgagaaata catatcagga
     2581 ctatccctta cagtggctgg cgaacaggca ggtattgaaa agacacattc tgtgatgggg
     2641 cgcattttga aaaacgtcaa ctaccttgga aatgatacgt atccagcaat tattgataaa
     2701 gagatatttg ataaagctga agaagttaga gataaacgtg caaaggattt aggacgagtg
     2761 gtagagcttg ccgctttcac ctctccccct cccaaagaac gattcaaaat gaaaaaggca
     2821 gataataaga tgccagtgga tccttttgaa cgagcagaat acttatacag tctgatagaa
     2881 agcgaggaat aaagtgacag agaaaaatat aatggttatt cctgctcgta aaagagtagg
     2941 aagtacagcc gcaaaagaaa agataaagaa acttcgtgtt gctgcctatt gccgtgtttc
     3001 tacagaaaca gaagaacaaa attctagcta tgaggttcag gtctcacact atacagagtt
     3061 tataaagaaa aataatgaat gggagtttgc tggcatcttt gcagatgatg gtatctctgg
     3121 tacgaacact aaaaagcgtg acgaatttaa tcgcatgatt gcagagtgta tggacggtaa
     3181 catcgacatg gttattacaa aatccatcag ccgatttgcg cgtaacaccc tagactgcct
     3241 tcaatacatt agacagctca aggataagaa catatcagtt tattttgaga aagagaacat
     3301 caacaccacg gatgccaaag gtgaggtttt gctgactatt atggcatctt tggcacagca
     3361 ggaaagccag agcctttcac aaaacgttaa gcttgggcta cagtaccgat accaacaagg
     3421 aaaggtgcag gtcaaccata agcgatttat gggctacacg aaagatgaag atggaaattt
     3481 aactattgtc cccgaagaag ctgagattat caaacgcatc taccgagaat accttgaggg
     3541 tcagagtcta gtgggcattg gtcgagccct tgaaaaggat ggtattttaa cagcagcggg
     3601 aaaaccaaga tggcgaccag aatcagttaa gaagatactt caaaacgaaa aatacatcgg
     3661 agatgccctt ctgcaaaaga ctgtcacagt agattttctg accaagaaac gagtgaaaaa
     3721 tgaagggcat gttccccaat attatgttga aaatagccat gaagcgatta ttcctaaaga
     3781 gttatttttg caggcgcaag aagaaattca tcggagaagc aatatctaca caggagaagg
     3841 caagaacaaa agaatttaca gtagcaagta cgctttaagt gccatcacct tctgtggaga
     3901 ttgtggcgat atttatagac gggtctattg gaatatccac ggaagaaaag aatttgtctg
     3961 gcgatgcgtg actagaatcg agcaaggtcc tgaagtttgt aagaaccgaa ccgtaaaaga
     4021 agatgaactt tatggtgcag taatgactgc gattaataag ctacttgcag gtgggaacaa
     4081 tatgataaag acactggaag agaatattca tgctgtgatt ggcgaaacca cagaatacca
     4141 aatttcagag attaacaact cactagagga aaaacaaaaa gaactaatca agctggcgaa
     4201 caagggtcaa gactatgacc acttagcaga tgagattgat gagctgagag acaagcgaca
     4261 gatcctttta gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat
     4321 tgaatttatc cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa
     4381 gataatccag agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat
     4441 cgaaatggaa atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg
     4501 ttttcttttg cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat
     4561 ggtttataat attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta
     4621 aagaactgtg tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga
     4681 ctccacagaa tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg
     4741 ccattgctga tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag
     4801 aaataaaaat atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat
     4861 agtaacagag aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg
     4921 tgcaagaggc acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga
     4981 ctcatttgac ctgacagcga ttaatcaaat tgctacagag ttggatgatg agaatagaaa
     5041 caaccttgtt gtacctcccg gagcgtgggg tgattgggtt aatggcggtg gatggttagt
     5101 tataaacggg tgtcatgttg acttgatatt acgtgatata aaacgggtgg aacaaataat
     5161 caaagatacg gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta
     5221 tataagtgct atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga
     5281 aagcttatgc gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt
     5341 gatgaacttt tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg
     5401 ggcagaggat aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca
     5461 agtactattt gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct
     5521 tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt
     5581 actcggtctt tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa
     5641 tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca
     5701 aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac
     5761 tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc
     5821 gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg
     5881 cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa
     5941 cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg
     6001 agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt
     6061 taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac
     6121 atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac
     6181 tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag
     6241 aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata
     6301 gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg
     6361 ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt
     6421 aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga
     6481 tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc
     6541 acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta catattttgt
     6601 aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat
     6661 aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta
     6721 tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt
     6781 agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat
     6841 taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga
     6901 gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg
     6961 ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct
     7021 gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa
     7081 ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta
     7141 ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag
     7201 ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa
     7261 tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga
     7321 tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca
     7381 gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata
     7441 ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta
     7501 aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt
     7561 gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt cggaaaagtt
     7621 aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt atgtaaggat
     7681 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     7741 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     7801 tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     7861 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     7921 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     7981 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     8041 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     8101 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     8161 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     8221 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     8281 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     8341 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     8401 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     8461 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     8521 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     8581 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     8641 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     8701 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     8761 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     8821 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     8881 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     8941 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     9001 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     9061 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaaat
     9121 aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag tttacagaga
     9181 tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa aaggatattt
     9241 tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag tgggtgcatt
     9301 gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa aggattttga
     9361 ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact atgcacagga
     9421 gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat tggcggataa
     9481 cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa ttcaatatcc
     9541 tgcatcagga gggcaagaac cggaaagcag aatgatagag cctatatcac tggggtttta
     9601 tgaacagaac tggtacttaa tcggttttgc aggctaagaa atgaatatag gaattttcgt
     9661 gtggatcgga ttaaaagcat ttgcatagaa gaagagctct gtcaaagcca tgatggctct
     9721 ttggaacaaa tattaaaaca aatgctttca tataaaaaac tata
//
LOCUS       DASJRQ010000096         9665 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJRQ010000096 DASJRQ010000000
VERSION     DASJRQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.097"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..648)
                     /locus_tag="U4A80_002267"
     CDS             complement(<1..648)
                     /locus_tag="U4A80_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1975876.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            973..1389
                     /locus_tag="U4A80_002268"
     CDS             973..1389
                     /locus_tag="U4A80_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1975877.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1386..1856
                     /locus_tag="U4A80_002269"
     CDS             1386..1856
                     /locus_tag="U4A80_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1975878.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1870..2643
                     /locus_tag="U4A80_002270"
     CDS             1870..2643
                     /locus_tag="U4A80_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1975879.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2657..3469
                     /locus_tag="U4A80_002271"
     CDS             2657..3469
                     /locus_tag="U4A80_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1975880.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3523..4488
                     /locus_tag="U4A80_002272"
     CDS             3523..4488
                     /locus_tag="U4A80_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1975881.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4604..7327
                     /locus_tag="U4A80_002273"
     CDS             4604..7327
                     /locus_tag="U4A80_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1975882.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7340..7972
                     /locus_tag="U4A80_002274"
     CDS             7340..7972
                     /locus_tag="U4A80_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1975883.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8871..>9665
                     /locus_tag="U4A80_002275"
     CDS             8871..>9665
                     /locus_tag="U4A80_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975884.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1475 c         1884 g         3045 t
ORIGIN      
        1 aatcatatgg aagccaccga ttaggtattt tatgggttta gtcggaaatg tttcaagagc
       61 aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa tcagaccgtc
      121 ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa atttatcatc
      181 catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga tgatatggaa
      241 gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca ttttaaggat
      301 ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat aatggggtaa
      361 agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc ttaacccgcc
      421 ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca aagtttcccc
      481 cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga gatatttctt
      541 ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca aatttattct
      601 tgagttttca aataaaacct tgatttccac tataacatct cctttcacat gaacgtgtat
      661 tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa aaatattaat
      721 ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg tggcggggga
      781 ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt taaataccca
      841 cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat agaaaaacct
      901 ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag cgtaaggata
      961 ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc tcaaggatta
     1021 aagcaaacag ctacaatgat tattggagat agtgtaccaa ttcatgcctt gtcagcattt
     1081 agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac aatagatata
     1141 gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga tatgctcact
     1201 attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc tttagttatt
     1261 tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct tattaaagag
     1321 agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac tggaggagat
     1381 gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag gtagtatttt
     1441 cttggagtaa atcattaaat atcaatcgta ttattattgc caataaagag gcagcgaatg
     1501 atgaattcaa aaaaatgtct ttgaatttat ctaaaccgac tggcattaaa ttaacaattt
     1561 tttcagttga acagttacta gaaaaaatgc ctaaaatcga aacacttaac gaaaatatca
     1621 tgcttatttt tggtaataca aaagaaattc gtcaattttg tgaagcctat cctaaagtac
     1681 aagaaatcaa ctatggaggg ataatcaaaa aagaaggttc caagcaattt agtaatgcca
     1741 ttttcttgaa tgaatctgag caagaagatg ccaaaaaatt aaaagctctt ggcattaaac
     1801 aatttattca acaggtacca acttctaaaa aagaagattt gaatacaatg ttataaagga
     1861 ggaatgaaaa tgctcgttca gtcaattggt ttagggttaa tcggtgtttt ttgtatttta
     1921 gactcaaggc ttttaggaag aatgaatttt gaacgaccgt tgattgtcag tacgcttgtt
     1981 ggtttggtac ttggagacat ggaaaaagga ttaattgttg gagcctcttt ggaattgatg
     2041 tccttaggat tagtaaatat cggagctgct gcaccacctg atatgaatat ggcatcaatt
     2101 attgctacag cctttgccat tttatcaaat gcgaatgcag aagcagcatt aacgattgcc
     2161 attccaattt cagtgttagg tcaaatacta ggtatcttaa tgcggacgat tttgtcaaat
     2221 ttgactcacg ttgcggatcg ctttatcgaa caagggaaat ttagccaagc aaggagaatt
     2281 catatacttt atggctcggg attatatgct ctcatgtatt ttattcctat ttttctagca
     2341 atttattttg gaacgaatct tgtttctcag attgttgatg cgattcctga gtggctaaca
     2401 aatggcttaa cggttgcaag taaaatttta ccagcatatg ggtttgcgtt gttattacaa
     2461 acgatgttaa cgaaaaaaat ggtgccattt ttattacttg gatttttaat tactgcttat
     2521 tctggattgg gaatcattgg tattgcagcc ttcgcatgta ttgttgcgtt tgtcatggtt
     2581 caagttcaag gtaaaaactc aaatggtaac caaaatgagc cagatccatt agatgactta
     2641 taaggaggta gattaagtga aagagaaaga tcaaaatgca tggaaatatc ggcagttctt
     2701 atggcgttca tgggcaattc aagcctcatg gaactatgaa cgacaaatga acatgggttt
     2761 tatgtacgga attgcaccaa ttctagataa catttacaag aatccagaag atgaagcaat
     2821 gaagaaagaa gcttacgagc ggcatatgat gtactacaac tgtacccctc aaacaagtgc
     2881 atttgtgtta gggttaactg cttctatgga ggagcaatac tacgaagacc aagaaaactt
     2941 taatccggaa tcaatcagtg cagtgaagac ttctctgatg ggaccgctat ctggtgttgg
     3001 tgattctttc tttcaaggaa cgattcgggt attagccttt ggactaggaa ttaatttagc
     3061 ccaacaaggt agtattttag gtccaattct agcaatgtta atttcctttg taccttcctt
     3121 tttagttact tactatggtg ggaaaattgg ctataacatg gggaataaat acttgtctaa
     3181 actgtatcaa gaaggtttga tggataaagt aatgtatgtt tgctccattg tggggttgat
     3241 ggtcattggt tcgatgattg ccagcatgat cgggattaca acaccaattc aaatgggtaa
     3301 agcttttgtc ttacaagatg tattagatag tattatgcca caatttttac cattaggatt
     3361 aaccttcttc atgtactggt tattgcagaa aaaggtcaaa acaggatgga tgttggcaat
     3421 ctgtttagtc ggaggaatta tttttagttt gttaggtatc ttacaataag ggaataacca
     3481 ataaatttgg ttgaataata taaataagga ggaattcaat tgatgaattc aaaagaaatt
     3541 gttaaagaaa tcgtagcgaa aaaagaggta aaatcaatcg tatttgttgg ctgtggcgct
     3601 tcaaaagcgg acctttatcc tgcaaagtat tttctagacc aaaatgcgaa gcagttacgg
     3661 attagtcatt acacagccag cgaatttaac tatgcaacac cagcatctgt agatgagtca
     3721 actgtcgtta tttcagcttc tttaggaggt acgactccag aaacagttga agctaacgca
     3781 gtagctaaag aaaaaggtgc tacagttatt agtctaacac gaagcattga ctcaccgtta
     3841 acaaaagatg cagattatgt aatttatcat cgatttgcag agaactatgc tgctaaatta
     3901 gaaaaaacag gctactgttt agaattggca gtagaacttt tacatcaagt agaaggttat
     3961 gaccattatg aagcaatgca agaaggattt gctactatct atgatcttgc ccaaaaaagt
     4021 gcagaatcag ctcgtaaaga tgcgaagaaa tttgctgaac gctttaaaga tgaaaaagtt
     4081 atttatgtaa tgagtagtgg tgctacacat gaagtagcat actcaacatc aatttgcttg
     4141 atgatggaaa tgcaatggat taattctggt accttccatt caggagaatt tttccacgga
     4201 ccattcgaaa ttgtggataa agacgtaccg tttatattat taatgaatga tggaaaaact
     4261 agaaatatag atgctcgagc attaactttc cttgaacgct ttgatacgaa aacagaagtt
     4321 gtagatgcct tggattatgg cttgtcttca catattgcta aagaagtctt agactacttt
     4381 aacccattcg tgattactgc agtatttaga gtttatgcag aagaattgtc agaagcaaga
     4441 gaacatccat taactaagag acgttatatg tggaaactaa cttattaaaa aatagatcaa
     4501 actaaataag gtggcttgag aaattaagcc accttgtttt cttcaatgtg attttataca
     4561 tatggtaaaa taactagtac atattgtgaa agcaggtggc aagatgcagc gattaaatga
     4621 ctatctttat caactaacat tgcttttgaa atctacgcct tcagataatt attcagctcg
     4681 cagtatttcg caagctttac agttgaatag aagtacaatc agtagctact taaatgaagg
     4741 tgttcgtgaa gggcttttca taaaagtaaa aagttacccg gttttatttt tacatagaac
     4801 agctcttgaa gaactacata tcacactgaa taactccgaa atagaatcaa ttgaaagctt
     4861 gttaacaatt tctcaaaaac ccgcattaga tcaagtaatt ggctctaaag gaagtttgaa
     4921 agaagcaatt gatcaaatta aaacggcagt cttatatcct ggaaaagggt tgcctttatt
     4981 attgatcggc gctagtggat cagggaaaac ctttctagcg aataaaatat atgaatatgc
     5041 agtagaagaa aaggtcattc acacatcggc acctttcata gattataatt gtgcacaata
     5101 tgtgagcaat ccagagttac tatcctctgc tttatttggc tacacgaaag gagcatttac
     5161 cggagcaagc caggagcata ctggtttgct agaaaaagcc gatggaggag tattgttttt
     5221 agatgaagtg catcgcttat ctgaagaagg gcaagagaaa ttatttacat ttatggatac
     5281 gggtgaattc tctcctatgg gggataatag tattcggaaa aaagccgatg ttcgattagt
     5341 ctttgcaaca acagaaaata tatatacgac tttcttacca acttttctta ggagattgcc
     5401 agttattgtt aatctaccta gatttcaaca acgtccgtcg tttgaacgtc tgtcattgat
     5461 tgatgaattt tttgtttctg agagtaaaat tttagctaag gaactatctg tttctgatgc
     5521 attgattcat ttcttaatga atagtgattt tgaaggtaac gtggggaata ttaaaaatat
     5581 tatcaagtat gcttgcggaa acgcatatat ccaccagaag agtcagcaaa caattcaagt
     5641 taaattattg gatatgcctt tagagtacag ttataaattc aaggagcaac taaataaacc
     5701 cagaaagaaa agagcagatc gaatctacta tccagaagaa acacgacaag tgcaactaga
     5761 aagaaacaat cagctttttg ttcaattttt tgaccaatta cttactggat tctctgaagt
     5821 gattgaaaat aacaaggata ttaaactatt tttagaagaa atggttacca aggtaacaca
     5881 agtaatggat acgcttattt ttcaagagga ttacgaaaaa gagcagtctt tatacgccat
     5941 tctaaactat catattcgtc aaagtattga ttttatgcga gaaagctacg ggtttgaaca
     6001 agatggtaac cgggtagtcg ctttggccaa ctatctatat gtcaaggata atcaagtgat
     6061 ttttgaaaat agtcttcaag aaaagaaaac caaagatcag cttaacgaat tttttgctaa
     6121 tgttcttgaa accccttatt ggtatgcaaa aaaaatctta agccttttat ctgagtgttt
     6181 agatcaacct gtgtatgaag aggacatttt ctttattacg ctatattttt atagtttgaa
     6241 tgtcacgaat gttaaaacag gtattaagag tattgtgcta gctcatggtt attctacagc
     6301 tagtagtatg gcgaatgttg ccaaccgtat gctgaataag aacgtttttc aagcttatga
     6361 tatgccttta gatattacat tagagaaaat tgaagcaaaa ttgattcaat tccttggaga
     6421 ttatcgaaca gattcaggct tgattttatt ggtagatatg ggttcactaa accaactagg
     6481 cggtttatta gtgaatcatc taaatggacc actgttaatc attgatcata tcaatacacc
     6541 gttattattg gaagtcggcc aacaagtatt aaacaataaa agtatcaccg aaataggagc
     6601 agaaattgaa acaagtctta ctttcaaaaa gcagttacta cttcccaaga aaaagaaaaa
     6661 gaaagccatc attacttgtt gttatacagg gattggtagt gcgacccaga ttcaggagat
     6721 tttaacgaaa tgcttgaaag atgcagttag agaattagta attattccgt atgattacaa
     6781 aaagctgtta gaaaacaaga attttgagac tccttttcaa ttatacgatg tcatcatgat
     6841 tgtaggtaca gaagatccca aaattaatca gattccatat attggattag atcgcctgat
     6901 taatggggaa gcaattggcg aatttgttca ttacttggaa aaagagctga ctattgaagt
     6961 gaacgattta caaaaagaat taattttcaa tttttcaatt agtaaaattg tcgaaaattt
     7021 aaccattttg gatccagata aggtgctatc ttttgtacaa aaagcaatta aagaattatc
     7081 gaagttatta aattttgatt tttctaataa tcagctgttt ttgttgtacc ttcactgtag
     7141 ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat gagcaagagg acattgaaga
     7201 atatatgcaa gcaaatggag aaataatgga taaaattcat agcgcttttt ttgagattga
     7261 aagggaatac acaattgaaa taccagattt agagttgcga ttattgagcg atattgttca
     7321 aggataggag tgaattcgaa tgacaatggg agtaattttt gatatggatg gggtactcat
     7381 agacagcgag acattttatt ttgagagaag aatgaacttc tttcaagaac tggagataga
     7441 tccaggatca aaccagattg atgattatgt ggggaaaaca gaagctgcaa tatgggaaac
     7501 attagttcct gaggacaaat cattaagaga ggaactttat ccgaaatatg tagattatcg
     7561 aaaaacgcat ccaattgatt tcgctaaaga tttacgaaaa gaagtgaaac cgttacttga
     7621 cttcctcaag aagcgggata taccattggc actggcatct tcttctccag ttgttgagat
     7681 agagagtatg ctcattcaat gtgaacttac tgaatatttt tcctttgtta taagtggtga
     7741 gtcattaaaa caaagcaaac cacatcccga aatatattta aaagcgaaac agcaattaga
     7801 ctgtgacttc tttattgcag tagaggattc aacgattggt attaaatcag caaaggcagc
     7861 aaatatctat acggtggcac taaaacaacc aatgcaaatc gatcagagtg aagcagacat
     7921 acaaattgaa agtctacaag aactggaaca aattgtttta gaaaaaggtt agtgaattaa
     7981 tggaaataag gctatatatc tgtgctagct attttgacta accaaaaaaa cttgctataa
     8041 ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa aatgcttcgg cagtgataat
     8101 gggattggct ttttaagttt tttattttgt tcgcaatccc taaggtcatg tgaatgatcc
     8161 tagggattgc tagaatattt tgcaacattc tatctatttc agaacgtaac cttccccagt
     8221 tcattgggat tcattagcat acaaccaaca tttcaaaaag aaattgcact acaccattat
     8281 tagctatttt gactaacccc aaaaaacttg ctataattca ggtgtaggta aaaggtgctg
     8341 gtatcacctt ttacgaaact gcttcggcag tggtaatgag tgtggcttaa aggactagtc
     8401 ctttaagctt tttactttgt tcgcaatctc taagatcaag ttaatgatcg ataagattgc
     8461 tagaatattt tgcaacattc tatttcagaa cgtaaccttt cccattccac tgtgattcat
     8521 tggcaaccct cctcacaacc aacgtttcaa aagaaattgt aaagttgttg gggtaccacg
     8581 accgaacaat actacacctg aatgatagta agttgtctca ttaagtatag catatcctct
     8641 ttttgggtaa atagaaatcg gttacatttt ttatccaaga ccaagttttt tcatttggtc
     8701 ttatttgcta tacccaaaca attccgcagt atagtcgacg gtattgattt tatcaggtga
     8761 gtaatagaaa cttgttttcc ttccccaaaa cattacctat aaaggagaaa aaatggtatc
     8821 gtaattatat aggtatagga tgaaattttt ttctaagagg agggttgttg atgaaaaatt
     8881 tacagttaaa atttattgtt gatccatcga ttgtacgctg gtttcggatt atcaatcaat
     8941 ttgagcgcaa tcaacttgcc acaaaaggag aattagcaaa gagtaatcat gtatctgaac
     9001 gaaccattca aacagatgtc aataaaatga aggactactt ttctgaaagt gttcaatttg
     9061 tttcaacgaa aagtgggtat tcttttcaga aggaaagacc ggttcttttt ctcaagcaga
     9121 aggagcaact actggagaac gaaattttat ttgagttgtt agggaatgtc ttttatggcg
     9181 aattggaaga tatgacggag ctaattgatc gttttcattt atctgaagcg acttttcgtc
     9241 ggtatttgaa gcagattcaa ccagttttgg aagaatacgg cttagaattg agtctttatc
     9301 ctcttgattt aaaaggagaa gaggcaaata ttcgcaagtt ttttaaagac ttctattatg
     9361 aaggggagat gacgccccat actcttgttc caccaaggga tcttcatgaa cttgtgcaag
     9421 ctacattcta tgataaattt gagtatttct cgattggaac agggacttct cctgctgaat
     9481 tttattatag tttgtatatt gcgattgagc gtgtgagaca aggaaaaacg atagccattc
     9541 ctcaaggtct gattgagcga gtaattgcta gtgagaattt tcgcttaatg tattccttga
     9601 aagaagcgat tcaaacaaat tatcaagttg ctttatcaga agaggagttt tgttggttgt
     9661 attta
//
LOCUS       DASJRQ010000097         9592 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJRQ010000097 DASJRQ010000000
VERSION     DASJRQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.098"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="U4A80_002276"
     CDS             complement(<1..641)
                     /locus_tag="U4A80_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1975885.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="U4A80_002277"
     CDS             complement(643..1407)
                     /locus_tag="U4A80_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1975886.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="U4A80_002278"
     CDS             complement(1415..2092)
                     /locus_tag="U4A80_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1975887.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="U4A80_002279"
     CDS             complement(2129..3514)
                     /locus_tag="U4A80_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1975888.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="U4A80_002280"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4A80_002280"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U4A80_002281"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="U4A80_002281"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1975889.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="U4A80_002282"
     CDS             4453..5310
                     /locus_tag="U4A80_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975890.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="U4A80_002283"
     CDS             complement(5391..5726)
                     /locus_tag="U4A80_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975891.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="U4A80_002284"
     CDS             complement(5793..7163)
                     /locus_tag="U4A80_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1975892.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="U4A80_002285"
     CDS             complement(7241..7777)
                     /locus_tag="U4A80_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1975893.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="U4A80_002286"
     CDS             8086..9567
                     /locus_tag="U4A80_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975894.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3213 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt at
//
LOCUS       DASJRQ010000098         9494 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJRQ010000098 DASJRQ010000000
VERSION     DASJRQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.099"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1377
                     /gene="lysA"
                     /locus_tag="U4A80_002287"
     CDS             118..1377
                     /gene="lysA"
                     /locus_tag="U4A80_002287"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1975895.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1364..2164
                     /gene="dapF"
                     /locus_tag="U4A80_002288"
     CDS             1364..2164
                     /gene="dapF"
                     /locus_tag="U4A80_002288"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1975896.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2167..3048
                     /locus_tag="U4A80_002289"
     CDS             2167..3048
                     /locus_tag="U4A80_002289"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1975897.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3045..3761
                     /locus_tag="U4A80_002290"
     CDS             3045..3761
                     /locus_tag="U4A80_002290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1975898.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3767..4462
                     /gene="dapD"
                     /locus_tag="U4A80_002291"
     CDS             3767..4462
                     /gene="dapD"
                     /locus_tag="U4A80_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1975899.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4478..5479
                     /locus_tag="U4A80_002292"
     CDS             4478..5479
                     /locus_tag="U4A80_002292"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1975900.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5476..6837
                     /locus_tag="U4A80_002293"
     CDS             5476..6837
                     /locus_tag="U4A80_002293"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1975901.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6797..7948
                     /locus_tag="U4A80_002294"
     CDS             6797..7948
                     /locus_tag="U4A80_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1975902.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7923..9092
                     /locus_tag="U4A80_002295"
     CDS             7923..9092
                     /locus_tag="U4A80_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1975903.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9267..>9494
                     /locus_tag="U4A80_002296"
     CDS             9267..>9494
                     /locus_tag="U4A80_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1975904.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVH"
BASE COUNT         3121 a         1656 c         2141 g         2576 t
ORIGIN      
        1 tcgaaatata ctagacaatt cagaaaattt tgtttaatat tattccaata aaattcaaat
       61 ctttatcaaa tgaaattcca ataaaaacgg aaaaaaagtg aaaagaggaa tgaaaaaatg
      121 acagaattga taattggcgg agtcacagca agcacattgg cagaaaagta taagacacct
      181 ctgtatgtgt atgatgaaga aaagatgaga caaacgatgc tgtcattcaa gcgaggcttt
      241 gtatcgaaac gttttgatac aaaagtgctt tacgcctcta aagcattcca aacgattgag
      301 atgctgcatt tagttcgtga atatggattc gggctagatg ttgtcagtgg tggggaaatc
      361 tataccgctt tgcaagctga ttttcctaga gatactatct acttccatgg taacaacaag
      421 acgccagagg aactgatcta tgcacttgaa aatgatttgt tgcatattgt tgccgataat
      481 ttgatggaag ttgaattatt agcagaatta agccaacgct atttaaaaaa aatctgtatc
      541 atgcttcggt tgaatgtcgg agtagaagca catactcatg aatatatcgt cactgcgcat
      601 attgattcaa aattcggcat gagctatgaa agtaaagagt gccaacaagc ccttcagttg
      661 ataaaagaaa gcagctatct agagttagaa ggatttcatg ctcatatcgg gtcacaaatt
      721 tttgatgtta ctgcttggtt agctgaaatc gacaaattga cgaattactt aaccgatttt
      781 gaccaacctc tttccttgaa tcttggcgga gggtttggga ttcgttatac acaaaatgat
      841 cagccgatcc cagtcgaaga ggcactaaaa aaactgattg aatatacgga agaagctttg
      901 gaaaaacgaa aactgaccat tcgtcaatta ctgatcgaac cgggtagaag tattgttgga
      961 gaagctggta caacactata cacgattggt ttcatcaaaa agacaccaca taaaaaatat
     1021 tactttgtag atggcgggat gacagataat atccgtccag ctctgtatca ggcagaatac
     1081 gattgtgatt tagcagcaaa actgtcggaa gaaaaaacag agaaagtgac gattgcagga
     1141 aaaatgtgtg aatcaggcga tgtggtaatt aaagaagctt atttgccgaa agcagaatcg
     1201 ggtgatcttt tagctgttta ttccacggga gcttatggat actcgatgag cagcaactac
     1261 aatcgagcga cacgaccagc agttgttttt gtcaataatg gccaatcaag actgattgtt
     1321 cgcagacaag cattttctga tctactaagg ggggaagttt cctatgatcc ttcataaata
     1381 taatggctgc ggaaatgatt ttatcttatt agattacgca gaagacacag attatagtca
     1441 attagccgct tctttatgca caaaagaaaa atatgatata gacggattga tcgccgtaaa
     1501 aatcgatccg ttggaaatga tctattacaa caaagatggc agccgcgcgc cgatgtgcgg
     1561 aaatggcatt cgctgttttg ctcgatacgt aaatgatcag aacatgatga atgaacagga
     1621 attcaacgta gagacattag caggcacaag aaaaattacg gtgttggatc cctcaccttt
     1681 ttattgcagc gtagatatgg gagtgccaga tgattctccg gaaaaaatca aagctaatca
     1741 aacttatcca ataaaaaacc aggaaataga gatcaatggt gaaaaagtaa cgatcactag
     1801 tttattttta ggaacgattc ataccgttgt ttttgttgaa aatgcgcgtg aggaagtctg
     1861 gagaaaaaga ggtgaactga tctgtcatca tcctttgttc aaagaaaaaa ccaatgtcaa
     1921 ctttgtagaa gtagtcaatg aacaagaatt gatcgtccgt acctatgaaa gaggagtagg
     1981 ttggacactt gcttgcggta caggttgctg tgcctcttat gtggttgcta aggaaactgg
     2041 gaaaatttca gcagatcagg tagttgttca cttggaacaa ggagacttga tgatctctgg
     2101 tgaggatgcg atacaaatgg ctggcccagc agttcatgaa tggacaagaa tattggagga
     2161 atgaagatga taaaaggttc gatcgtggcg ctgatcacgc cgatgaatga agagggatca
     2221 gtagattatg ctggtttgga aaaattgatc caattccatt tagatgaaca aacagatgga
     2281 ttattagttt taggtacaac aggagaatct tctacgctca cacaatctga ggaggagcag
     2341 atccttcaac tgacagtaaa gaaagtggct ggccgtgtgc cggtgattgc aggagctgga
     2401 acgaataata cgaaagaaac gattgaaaaa gcgaagcatt ttgcttcttt gggggcagac
     2461 gcacttttgg tcatcacccc ttactacaac aaaacaagtg atgcaggact agctgctcat
     2521 tttacagcaa ttgcagaagc ctcaccgatt ccgcttattt tgtataatgt tccttctaga
     2581 acaggcatgt cgattccaat ccatgtatta gtaaatttag cagaacatcc taatatcatt
     2641 ggtttgaaag aagcctcagg agatatggct tatgtgatgg atgccgctcg gttgattgga
     2701 gaagaattct tcctttacag tggaaatgat gatttgatcc taccagtaat gagtgtagga
     2761 ggaagcggtg taataagtgt atgggcgaat atccaaccta aaatagtcca tgaattagta
     2821 aaagatacgc aggacggaag atggcagcaa gcaaaagaaa agcaattaaa tgcacttgaa
     2881 ctgatccatg ccttgttcag tgagaccaat cctattcctg tcaaagcagc catgtcatta
     2941 ttagacttac cttccggacc tctacgcctg cctttagttt ctctttcaaa agagaaaaag
     3001 aaacaattag ctcagctttt gttaaaagaa aggacggttg aaaaatgaaa atcggactga
     3061 ttggcagtgg acagatggga aatagagtcg aagaagttgc acaagaaaga ggagatacgg
     3121 tccttctttt acggacggca cctaaagaag caactacgct tgcttttacg gaactgccag
     3181 aactgcttat tgatttttct catccagata atttagagaa aatcctttcg tatagcttat
     3241 catatcaact tccgttagtc attggcacga caggttatac acaatcacag ttccaagaaa
     3301 taaaagaaca tgccaaacat gtacctgtga tgtatagtgc taatttttcg ttcggcatca
     3361 tggtgatgaa tcaactaatc aagcaagcag ctgcaatgtt gcaaggctgg cagatcgaac
     3421 tgattgaaaa acatcatagt cgtaaaaaag atgcaccatc tggtactgca aatatgctgc
     3481 ttcaaacaat ccaagaagtg caaaagctgc agcccgtata caactggcaa gggaaaaaaa
     3541 gagaagaaaa ccaaattggt gtacacagta ttcgtgcagg ttctttacct ggtgaacatg
     3601 atgtattgtt tgctgctact gatgaaatat ttacgatcaa acatgaatct ttttctaatc
     3661 gaatctttgc tgcaggcgca gtagaagcag cggactggtt gaaagatcag tcaccaggtt
     3721 attacaaatt agaagattta ttaatggaca aaggggtgta aaaaacgtgg aagctcaaga
     3781 aatcatccgc tacattcaaa cggcatctaa aaagacgcct gtgaaagtct atctgaatgt
     3841 aaccgagcca atcgaattta aagaaagtaa agtttttggt gaaggaagca gcttcgttgt
     3901 ttttggtgac tatgagacga ttgctccggt tttagaagct gaatcagaaa aaatcattga
     3961 aatcgaaatc gaaactgctg caagatacag cgcagttcct ttgctagata ttaaaaaaat
     4021 taatgctcgt atcgaaccag gtgctattat cagagatcaa gtgtcaatcg gcaacaatgc
     4081 cgtgatcatg atgggagcga ttatcaatat tggtgcagta atcggtgaaa acacgatgat
     4141 tgatatgggt gctgtcttag gtggacgagc gacagtgggc aaaaactgcc atattggtgc
     4201 aggagctgtc ttagcaggtg tgatcgaacc agcatcggcc aaaccggtta tcgtagaaga
     4261 cggcgtactg gtaggtgcga atgcagtgat tgttgaaggt gtccatatcg gaaaagatgc
     4321 agtcgtggca gcaggagcag tggttttaga agatgtggca gcagaaactg ttgtcggtgg
     4381 aatacctgca cgtgtattaa aaataagtga tgatcagaca aaagaaaata cagcattgat
     4441 tgctgcgtta cgcgaattat agaggagatg gagaaaaatg cgcgaaatag atacctgtat
     4501 tgtgggggca accggattag tcggccaaac gatgctcaaa gtgttagaag aatatgattt
     4561 tccagtagga cgattgaaat tattagcttc aaaacgttca gccggtaaag tttgtgtatt
     4621 caagaaaaag aaacacactg tacaggaatt gacagaaact tcatttgaag gatatgattt
     4681 agcgctattt tctgcaggtg cttctgtatc caaagagttt gcgccaatcg cacgaagtca
     4741 gggactattt gtcatagata attcctcagc atggcgggaa gattcggata ttccattaat
     4801 cgttccagag gtcaatctgg aagactatac attgaatcgg ctgattgcaa atccaaattg
     4861 ttcaaccatt caagcggtac ttccgcttaa agctttacaa gaagcctttg gtatccagcg
     4921 agtcaattat tcgacctatc aagcagtttc tggttccggg caaaaaggca ttgaagatct
     4981 tcatcgtacg caaaatgacg aggaaccagt tttgtatcct tataatatta gccaaacggt
     5041 gattccggaa atcgatcagc ctttggaaaa tgggtataca aaagaagaac agaaaatgat
     5101 aaatgagacc cgtaagattc tgcatcagcc tgctttgcca gtatcagcaa cctgcgtacg
     5161 tgtgccaatc gaaaatggac atggagtctc tgtggcagtt caactgcaaa aagagtttac
     5221 gacggaagaa attagagagt gtctcagcca gtttccaggg attcaattag aagatgatct
     5281 agcagcacat aaatatccga cgagcattct tgctagagga acagatatgg tttatgttgg
     5341 gaggatcaga aaagacactt ctttagagaa cggattgttg ttctatacga ctgccgataa
     5401 catacgcaaa ggagccgctg cgaatgccgt acagattgct gcagctttaa tgcagaaaat
     5461 agcggaggtg gcaagatgag taatacgctg aaagtagcga aattcggcgg aacatcgatg
     5521 tctcatgaag agcaattcaa aaaagtccgt acaattgttc tttcagatcc caatcgccaa
     5581 gtgatcgttg tttctgcttt aggcaaacgt acgtcagatg atgacaaagt aacagactta
     5641 ctttatttga tttatgctta ccttcagcat catgttgatt ggacacccct ttggaaaagg
     5701 atttgtgatc gtttcattca agtaagagat gttcttgccc tgcagttacc tattgaagat
     5761 gaactagcac gcatacagaa acaactggaa gacgaaaaga taactgaaga ttatttagta
     5821 agtagaggtg aatatctgac tgctcaactg atggctgagt atcttggtta ccagtttatt
     5881 gacgcaaaag acctgatttt ctttgactat gatgggaaaa tcgatcagga aaaaacagca
     5941 caggaaatca caaaagcatt taaaagatat ccgaaaatgg ttgttccagg gttttacggg
     6001 gcgaaccctg caggaaaaga aaaattattg ggccgtgggg gaagcgacat taccggcgcg
     6061 atactggcga agatgttgca agcggaaagt tatgagaatt ggacggatgt ttcaggaatc
     6121 atgatggctg atccgcgaat catcgatcat cctaaaatca tcgaggaaat ctcttttaga
     6181 gaactgagag agatggctta tatgggggct aacgttcttc atgaagcagc tgttttccca
     6241 gttcaagagg ctgatatccc catccaaata aaaaatacca atgatccttt cgctcaaggg
     6301 acaaaaatat cgaatcacga aatagaaaag gaaaatggac tgacaggaat agctggacgc
     6361 aaggattttc tttccatcac tttattcaaa cgtcatatgt ccgatgaaat cgggtttatc
     6421 tggaaagcaa tgagcatttt tgctaaacat gagatatcaa tcgaacatat tccttcaggg
     6481 atcgacaata tcggagtgat cgtctcagca gaagcgattg ccgataagtt atttctcatc
     6541 acgaaagaat taaaagaaac acttggcgtg gaagaaatcg tcgtgatcga agacttagct
     6601 ttgatctctg tcgttggggg tccgcataaa gaattgattg gattatctgg gaaagtgtta
     6661 tcgatattga acaaattaga aattcgcaca tcgatcttat cacaaggagc acaggagttg
     6721 aatctgatca tcggtgtacc gaataatcaa tatgaaacag tagtgaaggg tatctatgaa
     6781 gggatggtga atacggatgc tgaaagaata tcgaatggca ctccatcaaa ttcctgaaat
     6841 cgggtttcat gaattcaaga caaaaaaata tttatatgaa caagtaaaaa acagcgaggc
     6901 cgttatccat gaaatcggcg aaacaggact actcctttat tttgataatc agcaagaaga
     6961 aacgattgct tttcgaacag atatcgatgc actgccagtc aaagaagaaa cgaatctctc
     7021 ttttgcttct aagcatgaag gttatatgca tgcatgtggt catgacggac atatggcgat
     7081 gcttttaggg ttgactgact ttataactaa acacattgag gatcttattt ataatgtagt
     7141 actgatctat cagccatcag aagaaatttc cggtggcgca caaagcgtta ttcagtcagg
     7201 attgttggat aaatatgaag tacaagcaat atttggcttt catctatggc ctggactagc
     7261 taagggagaa gttttctctc gaccaggacc tttgatggca caaagcagtg aaacagacat
     7321 tatcattaaa ggccagtctg cacatattgc ttctagtgaa aaaggaatcg atagtctgga
     7381 ggcagctgta cgatttttga agaatgttta tgattttgac cggcaattac cggcagagtg
     7441 gaatcatcta atgaaatttg gccaaatcca aggtggaacg atccgaaatg ttttagctgg
     7501 agaagttatt gtatccggaa gtatccgttc ttacagttca gatatgcaaa attatttgaa
     7561 gaggggatta gaccatttag cagaacagtt tcactctaca gctactattt cttttcgcta
     7621 taatgacggc tatccagctg taatcaacca tagaggctta tatgcagcac tgttagattc
     7681 aaacaaacta catgaattag atgctccagt tcttcaagcc gaagattttg gcatatatac
     7741 gcaacaatat ccatgtgtat ttttcttttt aggtgtcggt gatacccctg ctttacatga
     7801 tccgacgttc gattttgata tggatgtttt agaaaatggt gtagaatggt acaaaacgat
     7861 tctttttaca gaaaatcttc ttgtaaaaat cgatagcagt tcaaaagagg agcgagtaaa
     7921 taatgatgaa tttatccaat cgatttagtc ctaggctttc tagaatagaa gtatcaaaaa
     7981 tacgacaatt cgatcagcaa atatcgtcga taccagatgt gatcaaactc accctgggag
     8041 aaccggattt tccaactcct gaacatgtca agcaagcagg aatagcagcg attgaagaag
     8101 acttttctca ctataccgga atgagaggat tagaggaact gcgggaagcg gcttgtatat
     8161 ttcagcaaca gcgttatgga ttaacttatg atccgcaaac ggaagttttg acgacagttg
     8221 gtgcaacgga agcgattgcc tctgcgttgt tatctgtttt ggaagaagga gacaaggttc
     8281 ttattccagc gcctgcttat tcaggatatc agccattagt ggaactagct ggtgctgaat
     8341 tgatcccgat agatacttct gacacagggt ttgtctgtca gccggaacaa tttgaacgag
     8401 catttgaaca atatggatca gctgtcaaag cagtcatttt aaactatcct aacaatccta
     8461 ctgggacgac attgtctgca aaacaattga aagcaatcgc tgaggtttta aaaaaatatc
     8521 cggtatttgt tataagtgat gaagtatatg ctgaattgac ttatagtgga acgcatatgt
     8581 ccatagctac ttatcttcca gaacaaacaa tcgtgataag cgggttatcc aagtcacatg
     8641 cgatgactgg ttggcgagtg ggctttattt ttgctcaaaa agcccttatt gacgaactaa
     8701 ttaaagtcca tcaatatctg gtgactgccg ctacaacgat gtcgcaaaaa gctgcggctg
     8761 aagcgctaat aaatggggtg aaagatagcg agaatatgaa agagcaatat caaacacgaa
     8821 gagactatct ggtccagcaa ttggcgcctt taggatttga agtgacccag ccaaacggtg
     8881 cattttactt gttttgcaaa ctcccagctg ccattcaaac tgactcttgg cagttttgtg
     8941 tagaattagc ggaacaggca aaagttgcgt gtatcccagg aattgcattt ggcccagaag
     9001 gagaaggtta tatccggatt tcttatgcta gcagcatgga aaaattacat gaggcctgca
     9061 aacggatcaa agacttttta acagaacagt gattcataga ttttatcaaa aaaacaatcg
     9121 ttaattttgc ggtatattct gcaaggagac gattgttttt taaatttagt tcaaatgaac
     9181 gcgtttcttt tgattcataa ctaatgtaat gttaagttta aattgtttag aatgatttta
     9241 aaagaatatt caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt gtaggtgcat
     9301 ctcatggagg gcatcaatcg atcttggaac tattgacacg atacggtgaa aatgtagata
     9361 tcacgctatt tgaagcagga gactatattt ccttcatgtc atgtggtatg gagctgtatt
     9421 tagaagacaa ggtaacagat gtaaatgatg tacgtaattt cagaccagaa aactttccgc
     9481 aatccaatgt tcat
//
LOCUS       DASJRQ010000099         9432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJRQ010000099 DASJRQ010000000
VERSION     DASJRQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.100"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..680
                     /locus_tag="U4A80_002297"
     CDS             <1..680
                     /locus_tag="U4A80_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1975905.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="U4A80_002298"
     CDS             800..1000
                     /locus_tag="U4A80_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1975906.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="U4A80_002299"
     CDS             1076..1273
                     /locus_tag="U4A80_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975907.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="U4A80_002300"
     CDS             complement(1570..3117)
                     /locus_tag="U4A80_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEN1975908.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="U4A80_002301"
     CDS             complement(3201..4004)
                     /locus_tag="U4A80_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1975909.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4A80_002302"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="U4A80_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1975910.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4A80_002303"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="U4A80_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEN1975911.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4A80_002304"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="U4A80_002304"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEN1975912.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="U4A80_002305"
     CDS             complement(6389..7318)
                     /locus_tag="U4A80_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1975913.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="U4A80_002306"
     CDS             7513..8196
                     /locus_tag="U4A80_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEN1975914.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="U4A80_002307"
     CDS             8357..9400
                     /locus_tag="U4A80_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1975915.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DASJRQ010000100         9278 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJRQ010000100 DASJRQ010000000
VERSION     DASJRQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.101"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..677)
                     /locus_tag="U4A80_002308"
     CDS             complement(9..677)
                     /locus_tag="U4A80_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1975916.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            811..1098
                     /locus_tag="U4A80_002309"
     CDS             811..1098
                     /locus_tag="U4A80_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975917.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1514..3052
                     /locus_tag="U4A80_002310"
     CDS             1514..3052
                     /locus_tag="U4A80_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1975918.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3049..4014
                     /locus_tag="U4A80_002311"
     CDS             3049..4014
                     /locus_tag="U4A80_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975919.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4001..5323
                     /locus_tag="U4A80_002312"
     CDS             4001..5323
                     /locus_tag="U4A80_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1975920.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5386..5490
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5564..6307
                     /locus_tag="U4A80_002313"
     CDS             5564..6307
                     /locus_tag="U4A80_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1975921.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6322..7314
                     /locus_tag="U4A80_002314"
     CDS             6322..7314
                     /locus_tag="U4A80_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1975922.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7326..8063
                     /locus_tag="U4A80_002315"
     CDS             7326..8063
                     /locus_tag="U4A80_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1975923.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8053..8775
                     /locus_tag="U4A80_002316"
     CDS             8053..8775
                     /locus_tag="U4A80_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1975924.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8859..>9278)
                     /locus_tag="U4A80_002317"
     CDS             complement(8859..>9278)
                     /locus_tag="U4A80_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1975925.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3035 a         1603 c         1962 g         2678 t
ORIGIN      
        1 aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt ttctgtagtc
       61 tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac tttattcaat
      121 gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag atccaaataa
      181 agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc aatcttaata
      241 tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct tccgccgaat
      301 cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact attgatcagt
      361 agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa gaaaactagc
      421 gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa caatgagata
      481 agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc aaaaagatta
      541 tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg aatattgatt
      601 cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa ttctgtaaat
      661 aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca acttctttat
      721 tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt ttggctgttt
      781 ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt gacaattctt
      841 acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga acaaaaactg
      901 gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt atttgaacac
      961 ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa ccgtgagaaa
     1021 gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa agagatagaa
     1081 aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt caaaacaacc
     1141 attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt tgaacaggcc
     1201 aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt ttattaaaat
     1261 aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg tatagatttt
     1321 tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg ttgcttggat
     1381 tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt gctgtaaggt
     1441 taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa agaataacag
     1501 aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag gcgagttgct
     1561 ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg cagcactgat
     1621 tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag tcttattgca
     1681 gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac ttatcccatt
     1741 tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt tgcttccgat
     1801 tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag aagcagcggt
     1861 agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca tcgcattacc
     1921 tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg ctactttggc
     1981 agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg accgaaatac
     2041 acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta tattcagcgg
     2101 actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt tagcagcagt
     2161 ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa atgaaaccat
     2221 cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt ataaagaatt
     2281 gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca agacgagttt
     2341 tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca caggtactgt
     2401 cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga caaaagaaaa
     2461 aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat tattaaaacc
     2521 aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga acgaacatca
     2581 acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg gattcacact
     2641 ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg gcttgaattt
     2701 ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata atggagatgt
     2761 caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc tagtgactct
     2821 agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga aaaaagattt
     2881 tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa aaataacaga
     2941 ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc cgacagaagt
     3001 agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt gactaaatta
     3061 atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga tgacctaaat
     3121 ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag tggaaaaaca
     3181 actactttga aaatgatcaa tggattatct aaaccatcag caggagacat ctacttcaaa
     3241 ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg atatgtgctg
     3301 cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt catccctgaa
     3361 atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct tcaaaaagtt
     3421 ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg aggagaacag
     3481 caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt gatggacgag
     3541 ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt tttgtcctta
     3601 cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga agcaatcaag
     3661 ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga cacaccagag
     3721 cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga accgaaacag
     3781 acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa tgaaatacca
     3841 gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata ttcgcttctt
     3901 tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc gctgacaagt
     3961 aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat atgatgcaat
     4021 catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag aagcaggtaa
     4081 aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc gtggatgtga
     4141 tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac aattgagtgg
     4201 aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat tcaaacgaac
     4261 atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg aaatcgatca
     4321 tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg aagaagtctt
     4381 ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac cagtggaggg
     4441 aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc ctaaagaaat
     4501 catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca atgctgcagg
     4561 gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg aggcgtcttt
     4621 ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt ttggtgttga
     4681 aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag agacggaatt
     4741 agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat ctttagcact
     4801 tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt atctgcaaac
     4861 gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc caaaattgac
     4921 accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg caacaagcga
     4981 acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac ttgctgaagt
     5041 aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg atcttaccag
     5101 ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga cctttgacca
     5161 gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat tgatcgatga
     5221 tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct atatcatggg
     5281 gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat gataactaaa
     5341 gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct gtttctgggg
     5401 atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct gtcagttaat
     5461 actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg tgcccatttc
     5521 aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga aaaatcttta
     5581 tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca tagtttggaa
     5641 aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag cggcagatgc
     5701 acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg gtttggtaat
     5761 agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt ttttagaaat
     5821 gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt tagggccatt
     5881 acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg tcttgataag
     5941 ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa aagagcaaat
     6001 catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat ataaaatacc
     6061 agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt gtgtcagcgg
     6121 agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca tgatacgcgt
     6181 aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca ttctattgag
     6241 tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta tgctttatat
     6301 tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct actgttgctt
     6361 ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa ggatttgaaa
     6421 aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat ctacctagca
     6481 aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga accaggagat
     6541 aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt aagttatcaa
     6601 gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc aattgctaca
     6661 gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat ccattcacca
     6721 aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga agctgtctta
     6781 aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt aggaagcaat
     6841 ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt tgaaggttgg
     6901 gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt gaaagaatta
     6961 gatgaacggt tagattacta tactccggta attattacga atgatcaact gatcaaaagt
     7021 gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca ggaagctatt
     7081 aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa tgatcggacg
     7141 tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc tgagaattgg
     7201 ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga aaacggattg
     7261 atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa ataggagtga
     7321 tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg aaaatattag
     7381 aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg ccaagcggag
     7441 ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact tctggaacga
     7501 tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat atgccgcaag
     7561 aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat caaaaaatca
     7621 atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt gggttaaacg
     7681 gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga acggccttgt
     7741 tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt ggagcgttag
     7801 atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa aaagcaaagc
     7861 ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca gacaggattc
     7921 ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct gaaaaacgac
     7981 ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat cagttattgg
     8041 caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca gagtttagca
     8101 ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc agaagaatgg
     8161 aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg tatccatcct
     8221 tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa agcaaggatc
     8281 atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac acaaaaaaaa
     8341 gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt actgcacaca
     8401 aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca ctatttgatc
     8461 cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg ttatttcagc
     8521 attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc agtcccaatc
     8581 gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt aggggggatt
     8641 ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat cgctgagatc
     8701 aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga acgtttcatt
     8761 agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt tttggctttc
     8821 tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc gtttgcttcc
     8881 cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact
     8941 ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgt
     9001 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
     9061 tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc aggagcaggt
     9121 tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt
     9181 tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca
     9241 gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DASJRQ010000101         9176 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJRQ010000101 DASJRQ010000000
VERSION     DASJRQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.102"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            528..1061
                     /locus_tag="U4A80_002318"
     CDS             528..1061
                     /locus_tag="U4A80_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN1975926.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1551..2075)
                     /locus_tag="U4A80_002319"
     CDS             complement(1551..2075)
                     /locus_tag="U4A80_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975927.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2068..2541)
                     /gene="tsaE"
                     /locus_tag="U4A80_002320"
     CDS             complement(2068..2541)
                     /gene="tsaE"
                     /locus_tag="U4A80_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1975928.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2664..3644)
                     /gene="pta"
                     /locus_tag="U4A80_002321"
     CDS             complement(2664..3644)
                     /gene="pta"
                     /locus_tag="U4A80_002321"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1975929.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3797..4477)
                     /locus_tag="U4A80_002322"
     CDS             complement(3797..4477)
                     /locus_tag="U4A80_002322"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1975930.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4603..5472
                     /locus_tag="U4A80_002323"
     CDS             4603..5472
                     /locus_tag="U4A80_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1975931.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5498..6013
                     /locus_tag="U4A80_002324"
     CDS             5498..6013
                     /locus_tag="U4A80_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975932.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6060..6653)
                     /locus_tag="U4A80_002325"
     CDS             complement(6060..6653)
                     /locus_tag="U4A80_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1975933.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6678..6857)
                     /locus_tag="U4A80_002326"
     CDS             complement(6678..6857)
                     /locus_tag="U4A80_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975934.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6934..7443)
                     /locus_tag="U4A80_002327"
     CDS             complement(6934..7443)
                     /locus_tag="U4A80_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975935.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7539..7919)
                     /locus_tag="U4A80_002328"
     CDS             complement(7539..7919)
                     /locus_tag="U4A80_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1975936.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8249..8998
                     /locus_tag="U4A80_002329"
     CDS             8249..8998
                     /locus_tag="U4A80_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1975937.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2665 a         1868 c         1644 g         2999 t
ORIGIN      
        1 gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg cactgcatca
       61 agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt tggacttatt
      121 accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca gcgtcctgac
      181 ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt ttttgagcta
      241 ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa agtcgcctac
      301 tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat aaatttgact
      361 taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga gttcaaaaac
      421 tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa tttatttgct
      481 tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg aattttgttg
      541 ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt gcgctagtca
      601 tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct gagactcctt
      661 ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc aatgcgccga
      721 aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt ttagtcgttg
      781 cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat tatcatcttg
      841 aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg tttcctgaat
      901 ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta gatcatcacc
      961 atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa gaaaaaaaat
     1021 tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact tatgaaacac
     1081 acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt tcaaaaaata
     1141 gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg agaacaccgt
     1201 ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
     1261 atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag cttttggaca
     1321 ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggc
     1381 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
     1441 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
     1501 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
     1561 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
     1621 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
     1681 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
     1741 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
     1801 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
     1861 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
     1921 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
     1981 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
     2041 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
     2101 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
     2161 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
     2221 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
     2281 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
     2341 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
     2401 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     2461 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     2521 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     2581 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     2641 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     2701 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     2761 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     2821 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     2881 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     2941 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     3001 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     3061 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     3121 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     3181 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     3241 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     3301 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     3361 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     3421 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     3481 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     3541 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     3601 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     3661 gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat tttatctgta
     3721 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     3781 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     3841 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     3901 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     3961 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     4021 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     4081 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     4141 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     4201 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     4261 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     4321 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     4381 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     4441 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     4501 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     4561 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     4621 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     4681 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     4741 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     4801 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     4861 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     4921 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     4981 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     5041 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     5101 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     5161 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     5221 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     5281 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     5341 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     5401 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     5461 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     5521 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     5581 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     5641 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     5701 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     5761 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     5821 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     5881 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     5941 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     6001 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     6061 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     6121 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     6181 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     6241 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     6301 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     6361 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     6421 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     6481 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     6541 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     6601 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     6661 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     6721 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     6781 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     6841 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     6901 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     6961 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     7021 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     7081 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     7141 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     7201 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     7261 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     7321 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     7381 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     7441 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     7501 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     7561 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     7621 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     7681 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     7741 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     7801 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     7861 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     7921 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     7981 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     8041 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     8101 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     8161 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     8221 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     8281 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     8341 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     8401 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     8461 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     8521 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     8581 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     8641 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     8701 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     8761 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     8821 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     8881 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     8941 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     9001 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     9061 cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     9121 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctct
//
LOCUS       DASJRQ010000102         8904 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJRQ010000102 DASJRQ010000000
VERSION     DASJRQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.103"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(453..950)
                     /locus_tag="U4A80_002330"
     CDS             complement(453..950)
                     /locus_tag="U4A80_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1975938.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(990..1799)
                     /locus_tag="U4A80_002331"
     CDS             complement(990..1799)
                     /locus_tag="U4A80_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1975939.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2133..2990
                     /locus_tag="U4A80_002332"
     CDS             2133..2990
                     /locus_tag="U4A80_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1975940.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2983..3885
                     /locus_tag="U4A80_002333"
     CDS             2983..3885
                     /locus_tag="U4A80_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1975941.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3918..4268
                     /locus_tag="U4A80_002334"
     CDS             3918..4268
                     /locus_tag="U4A80_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1975942.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4317..5240
                     /locus_tag="U4A80_002335"
     CDS             4317..5240
                     /locus_tag="U4A80_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1975943.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5318..6211
                     /locus_tag="U4A80_002336"
     CDS             5318..6211
                     /locus_tag="U4A80_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1975944.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6249..6812
                     /gene="amaP"
                     /locus_tag="U4A80_002337"
     CDS             6249..6812
                     /gene="amaP"
                     /locus_tag="U4A80_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1975945.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6826..7014
                     /locus_tag="U4A80_002338"
     CDS             6826..7014
                     /locus_tag="U4A80_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1975946.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7027..7929
                     /locus_tag="U4A80_002339"
     CDS             7027..7929
                     /locus_tag="U4A80_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1975947.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7961..8203
                     /locus_tag="U4A80_002340"
     CDS             7961..8203
                     /locus_tag="U4A80_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1975948.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8459..8830)
                     /locus_tag="U4A80_002341"
     CDS             complement(8459..8830)
                     /locus_tag="U4A80_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975949.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3091 a         1548 c         1736 g         2529 t
ORIGIN      
        1 ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
       61 aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
      121 aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa
      181 taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac
      241 ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg
      301 aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca
      361 atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt
      421 gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa
      481 tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca
      541 acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac
      601 aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa
      661 gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact
      721 cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg ttcttcttct
      781 ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt
      841 gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat
      901 tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct
      961 cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca
     1021 ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca
     1081 ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg
     1141 ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa
     1201 tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat
     1261 tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct
     1321 gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca
     1381 aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat
     1441 tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct
     1501 tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga
     1561 actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa
     1621 tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta
     1681 gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct
     1741 ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt
     1801 ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa
     1861 tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattcttaa
     1921 gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa
     1981 aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg
     2041 aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg
     2101 ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg
     2161 tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat
     2221 tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag
     2281 aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc
     2341 aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa
     2401 ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag
     2461 tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc
     2521 cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa
     2581 gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg
     2641 atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg
     2701 atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac
     2761 aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa
     2821 atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta
     2881 caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa
     2941 aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata
     3001 gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca
     3061 gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc
     3121 gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat
     3181 gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt
     3241 ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa
     3301 cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt
     3361 cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg
     3421 gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc
     3481 aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat
     3541 aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt
     3601 tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa
     3661 catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc
     3721 catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat
     3781 tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca
     3841 aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa gaccacaact
     3901 gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg
     3961 aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc
     4021 taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg
     4081 aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat
     4141 tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct
     4201 tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct
     4261 tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg
     4321 atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata
     4381 gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt
     4441 tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac
     4501 tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa
     4561 aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata
     4621 gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg
     4681 aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag
     4741 agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag
     4801 aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag
     4861 atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg
     4921 atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga
     4981 tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc
     5041 taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt
     5101 tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct
     5161 ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc
     5221 ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt
     5281 ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga
     5341 cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt
     5401 acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca
     5461 actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc
     5521 gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa
     5581 agatgcagag gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc
     5641 ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact
     5701 tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc
     5761 tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc
     5821 tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc
     5881 tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat
     5941 cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg
     6001 tgtagcacct ggtccaatct ggacaccatt acaactagac gaaggacaac cttctgattc
     6061 gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc
     6121 accagtttat gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg
     6181 agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag
     6241 gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat
     6301 ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga
     6361 agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg
     6421 ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa
     6481 tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg
     6541 aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg
     6601 tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag
     6661 cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat
     6721 tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta
     6781 aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt
     6841 tcgacataca agctccctat catctttggg gtacttggat tgatattagc cgttttatgg
     6901 atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct
     6961 atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa
     7021 acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg
     7081 tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac
     7141 aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta
     7201 gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct
     7261 aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat
     7321 ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga
     7381 aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca
     7441 atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt
     7501 gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa
     7561 cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta
     7621 gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa
     7681 atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc
     7741 aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg
     7801 caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat
     7861 aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg
     7921 gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact
     7981 tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat
     8041 ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt
     8101 aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat
     8161 cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga
     8221 ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc
     8281 atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac
     8341 ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc
     8401 aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt
     8461 aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata
     8521 cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa
     8581 agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
     8641 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
     8701 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
     8761 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
     8821 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
     8881 ttgtttgtga agctttttcg tgtc
//
LOCUS       DASJRQ010000103         8897 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJRQ010000103 DASJRQ010000000
VERSION     DASJRQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.104"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            233..1864
                     /locus_tag="U4A80_002342"
     CDS             233..1864
                     /locus_tag="U4A80_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1975950.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1867..2946
                     /gene="uxuA"
                     /locus_tag="U4A80_002343"
     CDS             1867..2946
                     /gene="uxuA"
                     /locus_tag="U4A80_002343"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1975951.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            3020..4447
                     /gene="uxaC"
                     /locus_tag="U4A80_002344"
     CDS             3020..4447
                     /gene="uxaC"
                     /locus_tag="U4A80_002344"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1975952.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4531..5178
                     /locus_tag="U4A80_002345"
     CDS             4531..5178
                     /locus_tag="U4A80_002345"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN1975953.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5298..6674
                     /locus_tag="U4A80_002346"
     CDS             5298..6674
                     /locus_tag="U4A80_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1975954.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            6684..7478
                     /locus_tag="U4A80_002347"
     CDS             6684..7478
                     /locus_tag="U4A80_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1975955.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            7491..>8897
                     /locus_tag="U4A80_002348"
     CDS             7491..>8897
                     /locus_tag="U4A80_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1975956.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEK"
BASE COUNT         3113 a         1144 c         1815 g         2825 t
ORIGIN      
        1 aagtaataca ctagttataa aaataaaaga atacatgaaa aaaagaaaaa ataagtttcg
       61 ataaaatcag tccaaaaaga aggagtattt tatttttctg aataaaaaat acaatcattc
      121 tgatatggta gaaaaaagca agtttattcc ctttttatat tgacaacggt tacaatgggt
      181 tttaaaatat aaaatgtatc aaaactaaca tatcagtttg aggaggttaa ttatggtttc
      241 gattaaaaat tggcaaaatt ctatcgaaga attcaatgaa atgcagattc tactgccgtg
      301 ttttgatgtc aaagaagtta agcaagaagg ccagcaacat ccagtatggc ttcattttgg
      361 tggaggaaat ctttatcgag gatttcatgc agaaattgct caatgcttag cagatcaaaa
      421 tgaattagaa acaggagtag ttgtgtgtga aacatttgat gaagaagtag tggaaaaagc
      481 ttatcatgac tataacaatg acattttgga aattgttatg agtggagatg gtgaactcaa
      541 aaaaagactt ttggcagtaa cggctgatag tttatattgc aatccgaaga atatagcgga
      601 ctatcaaaga gtggttaaat attttgaaga tcctaaatta cagtttacaa cgtttactat
      661 tactgaaaaa ggttacagtt taaaagattc taaagggcaa ttcttttcgg tagtcaaagc
      721 tgatatcaaa tctggaccta gcaatcctca acatacgatg agtattgtaa catccttgtt
      781 atatagcaga tttaatgctg gagagttgcc gattgcgatg gtttctactg ataatttctc
      841 acagaatggt caacgttttc aagatagtat ttttacgatt gcagaagctt ggcaaaaaga
      901 aggatatgtt tctgaaaaat ttattgctta tttaaaggat aaagaaaaag tgacttttcc
      961 ttggagtatg attgatcgaa ttacgccaaa tccttcagag aatgtaaagg aagatctatt
     1021 gatgatgggg ctcgaggatt tagatatcat tcacacaaaa aaacatacca atattgctgc
     1081 tttcactaat actgaggtag ttcattattt agttattgaa gatagttttc caaatggccg
     1141 gcctaaattg gaaaaggctg gtgtgattat gacagataga gagacggttg ataaagcaga
     1201 catcatgaag gttacgacaa gcttgaatcc acttcatact gctttggcag taacaggttg
     1261 tctgctagga tatcaatcaa tttcagcaga gatgcaggat gatgatttgg ttggtttaat
     1321 caaaggaata ggttatttag aaggtttacc agtagttgaa aatccaggca ttattgatcc
     1381 aaaacaattt attgatgaag taattaataa acgtttgcct aatccctata ttccagatac
     1441 accacaacga attgcaacag atacttctca aaaagtagcg attcgctatg gtgaaacaat
     1501 taaaaagtat gtcgaaaaag acggaaatgc agaaaatcta acatttattc ctatcgctat
     1561 tgctgcttgg ttacgttatt tactggcagt cgatgatcaa ggggaaaatt ttgtgccaag
     1621 tcctgatccg ttattggtag agttgcagga taaattatca gtattgaaat taggtgaaca
     1681 agatccttca aagattcatt gtgcggtaca ggaaatttta gaaaataaag aaatctttgg
     1741 tttagattta tatcaagttg gggtaggaaa gaaaactgaa aaaatctttt gcgaaatgct
     1801 agtaggtcct caagcagttc gtaaaacaat caataaatat ataacagaag ttggaggaaa
     1861 gtaactatgg aaatgacttt tagatggtat ggggcaaatg aagaaaaaat ctcactacaa
     1921 gaaattcgtc agatcccagc aatgaaagga attgttggaa cattaatgga tataccagcc
     1981 ggagaggttt ggccgagaga acgaattaaa tctttaaaag atgaagtcga agccaatgga
     2041 cttaccttga aagttattga gagtgttaat gttcatgatg atatcaaaat tggtttacca
     2101 acacgagatc aatacattga gaattataaa cagacgattc gcaatttggc agaatttggg
     2161 attgaggtta tctgttataa ttttatgcca gtatttgatt gggtaaaatc ggatttggat
     2221 tatcgtttag atgatgggtc ttctacgtta gcctatattt caaaaaatat ccctactgat
     2281 ccggaggaaa ttattagaga agtagaaaat tctactaata attttgaatt gccgggttgg
     2341 gaacctcatc ggttatcaca aattaagcaa ttattagaag cctataaaga agttgatgaa
     2401 gagaaattac gagagaactt tgcatatttt ctcaaagcaa ttatcccgac atgtgaagaa
     2461 gttgggatta aaatggcagt tcacccagat gatccaccat attccatttt cggattacct
     2521 cgtattgtta aaaatcgtga cgatttagat tggttgtgta atgtagtgga ttctccttct
     2581 aatggtatta cattatgtac tggaagtatt gcagaacgac cagataatga tgtctatgaa
     2641 attctatctg aattttgtca acgggatcgg attccttttg cacatgttcg taatatcaaa
     2701 tatttaagtg acaatggact cgatttttac gaagcgccgc ataaatcaga atatggttct
     2761 ctagatatgt ataagatatt aaaaacaatg tacgataatg gatttaatgg ctatattcgt
     2821 ccagatcatg gtcggatgat ttggggagaa accggtagac ctggttatgg attatatgac
     2881 cgagctctag gtgcagttta tttaaatggt atttgggaag cgcttgaaaa gggggcgagt
     2941 aaataattat ataagggagt ttatttagag gaaaaatttt ctcttctaaa aactaacgga
     3001 aggaaaggag tgtttaaata tgtttttaga taagaacttt ttattagaga atgaaatagc
     3061 taaaagactt tttcataaat ttgcaaaaca ccaaccaatt atcgactatc attgtcattt
     3121 agacccagca tatttgctag aaaataagca atacaagaat ttgactcagg tatggttaaa
     3181 tgataaagga gctggagatc attataaatg gcgattgatg cgagctaatg gtatagaaga
     3241 agagttgatc agtggtccag gtgatgatta tgaaaaattt ttagcttttg taaaaacagt
     3301 ggagaaggcg ccaggtaatc ctatttttga atggtctcat ttggaattac aacgttactt
     3361 tgatattgat ttgacaattt ctgagaagaa tgcaccagaa atttggcgac gtgctaacga
     3421 aaaaattcaa acggattcct atcgcccgaa agaattgttg gagatgatgg atgtgaaagc
     3481 catctgtaca acggatgatc ctacagatga cttgtggtat catactgagc tttctaaaca
     3541 ggataattct ttcaaggtat tacctacttt ccgtccagat aaggtctgga gtatcaaatc
     3601 atcaggcttt tcgaactata ttttaaaact tagtcaaaca gcggctatac caatcaaaga
     3661 ctttaatggg ttgaagcaag cattgagcca acgagtggat cattttcatc aaattggctg
     3721 tcgattagca gatcacggtt tgaatactta tttttatgaa atagcagatg aaaaaatact
     3781 cgacaatctg ttaataaaag ctttagcagg aaaaacggat ttccatttat cagaaattgc
     3841 acaatttgtt acaggtattc aattacattt gatgtctgag tatacaagat atggttggac
     3901 gatgcaaatg catttaaatg ctttaagaaa taattcaagt atattaaagg aacaaattgg
     3961 agtagatgtc ggtggtgatt cgatggggga tcaagctagt ttgacacaaa atgttgtagc
     4021 attgttcgat accgctgaaa agagtgggtg cttaccaaaa acaattttat attcattaaa
     4081 tccaactgat tggcttccac tagcaacagc aatgcaaagc ttccaagggg gtatgattca
     4141 aaaaatccaa ttgggatgtg cttggtggtt caatgataca gctgaaggaa tgcgtcaaca
     4201 gttgactacg atggcacaac aaagtttgtt gtcgaatttt acaggtatgt tgactgattc
     4261 tcgaagtttt ctaagctatc ctcgccatga gtattttcgt cgagtattat gtcaattact
     4321 aagcgagtgg gttaaagcag gccgaattcc agatgatgaa atgtatctta gtcagatagt
     4381 ggagaatatt tgttatcgta atgcgtataa ttattttaat ttttttgaaa tcggaagtag
     4441 aaaataatac ttgaaataaa agcttaaatt tattctttgt aagttagtac agctagacaa
     4501 agtatgtatc cataaaggaa ggagatagat atgaaaaaag tagaaatttt aagtaagtta
     4561 gaagcggctg gagtcattgc ggttgtacgt gggaaaaata aggatgaggc cattaaagca
     4621 ataaattcaa ttattgcggg tggaattaaa gggattgaac taacttttac agttcctaat
     4681 gctgaagaaa taatcaaaga aattgctaaa aaatatgctg atcaaaaaga tgtaataatc
     4741 ggagccggta cggttttaga tgcgtttact gctcgtatcg caattatggc tggggcagaa
     4801 tatatcgtta gtccatcttt tgatcgacaa acagctgaga tttgtaattt atatcaaatt
     4861 ccttatttac caggttgtat gacaattact gagataaagg aggcacttac tgctggcgta
     4921 gatatcatta aactattccc aggttctgct tttggtccat ccattgtaaa agcctatcaa
     4981 gctccattac cacacgtaaa tataatgcct actggcggag taagtataga gaacttagaa
     5041 gattggtttg aagtaggagt tgttgcagtt ggtgtaggtg ggaatttatt ggcttctgtt
     5101 gaaaaaggtg attttgaaga agtaagtcga gtagctagta aatatataga taagttttat
     5161 gagataaaga attattagga gaaaaaacaa ataatactct gaaatgtaaa aaataaatac
     5221 tgactcttta cgcttcagag tatagtacgt actttcatca gaatgaaaac gcttaacaac
     5281 agaagggaga gaattgtatg gaaagaaaat ttggtataag agacaaaata ggatatatgt
     5341 ttggagattt tggaaacgat tttactttca tttttgctag ttctttttta atgatctttt
     5401 atacaaaagt attggggata agcggagctg ccgttggaac attattttta gtagcaagat
     5461 ttatcgatgc agttactgat gtaactatgg ggcgtattgt tgatagatcg aaagcaacta
     5521 agcagggtaa gtttaaaccg tggattttaa gaatgagtgg tccagtcgct ttagcaagtt
     5581 ttttaatgta tcaaacttct atgatttcag cttctatgag cgttaaggta gtatacatgt
     5641 atgttacgta tatattatgg gggagtattt tttacacatc tataaatatt ccttatggat
     5701 caatggcatc agctattact tctattacag aagaaagaac aatgctatcg acattccgaa
     5761 atgttggagc tactttagca agtttggtca ttggtgtatt aacaccatta tttatttata
     5821 caagagatga ttcaggcgct caaatagttc gtggtggatc aacttttaca attgttgcgg
     5881 gaatattttc gatatgtgca cttatttgtt atttaatatg ttataaacta actactgaac
     5941 gagtaagaat tgaccaatct caatacaaag tttcattgtt aaaatcttta aaagggttag
     6001 ttaaaaatag ggcattactt tctataattg gtgcggctat tttcttactc cttgctcaat
     6061 tattaattat gagtatgaat aattatgtat ttcctgatta ctataatgat gcacagggaa
     6121 tagttttaat gaattttatc aatcctattt tggttttaat agttgtttcg cctttaactt
     6181 tgtttttaag taaaaaatat ggaaaaaaag aactggcatc agtaggaatg ttcttttcag
     6241 caatagtata tttgatctta tttttgataa aagctagtaa tatgtatgta ttcttaattc
     6301 ttagttcggt tggctatatg ggtttgggtg tgtttaatac tgttatttgg gcaaatatta
     6361 ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag agaagatggg accgtatatg
     6421 cggtatattc ttttgctaga aaaataggtc aagctctagc tggaggcgtc ggaggttgga
     6481 ctctttctat tataggctat gactctttag caaatgtaca aacgggagat gttttagaaa
     6541 agttatataa cgcttcaaca ttaattccat caatttgttt tctgattgta ggattaattt
     6601 tatttttcct ttatccatta tctaaagata aagtcttaga aaattgcgag cttttaaaac
     6661 aaaaacataa ataggagaga aatatgaata attggatatt ttgtattgat tcagatggct
     6721 gtgttatgga tacgatgact tataaacaca atctcttttt cggaccaatt gctgcagaga
     6781 tttttgatgt tcatcataga gaaaaattcc tacaggattg gaataatatt aatttatatt
     6841 cagagactcg tggaataaac agatttttag gtttagttaa aacattagaa aaaaatgatg
     6901 taacatcgat taatcgatta aagcactggg tatctctaac aaaagaattg tctaatccat
     6961 ctttgaagag ggaaatagaa tattctcagt cagaggattt attaaaagct ttaaaatggt
     7021 cggaagaagt caatcaacag attagtcaag caaaaggatt agataaaccc tttttaagtg
     7081 ctagagatac tataaaagct ctgaaaaaat atgggaagat aattattgta agctcagcta
     7141 ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt attatcatta gttgatgaac
     7201 tgtgttgtca agataaagga aaaaaagaag atattattcg aagtgtaatt gaaaatggtt
     7261 gtgatttgga taagatatta atgattggtg attctcctgg tgatctagaa gctgcaaata
     7321 aaaataaagt tttcttttat cctattttgg tcaataaaga aaaagaatca tgggaaaact
     7381 taagaactga tgttttattt aaatgtttat cgggagatta tgtagatatt gaacaaaaat
     7441 atatagatac tttttggaaa aatcttacta ataagtagag gagaaagaaa atgactataa
     7501 aagtagattt acgaaaaaaa ccatataatt tagatgaaga tgctatttct tggattgaag
     7561 agacgattga ttctatgact ttggaagaaa aaataggtca attgtttgtt aatatgggat
     7621 ctagtcgtga taaaaattac ttgagtaata tggtcaatca gtaccatatt ggagctgtac
     7681 gatataatcc aggaactgct gatgaggtat atgatcaaaa ctatatctta caaacagaaa
     7741 gtaaaattcc tctattaatt gctgctaata cggaagcagg tggtaacgga gcttgtacag
     7801 atggtactga agttggtgta gaagtaaaaa ttggtgcaac aaatgatgcg aaatatgctt
     7861 atgaattagg gagagtttct gggattgaag catcggctat tggatgtaac tggagttttg
     7921 ctcctattgt agatattaat agaaattgga gaaatcctat catttctagt cgatcttttg
     7981 gacaaaaccc tgataaagtt ttagaactta gtttagctta tatgaaaggt attcaggaga
     8041 gtggaatttt gcctgcagct aaacatttcc ctggtgacgg tattgacgaa cgagatcagc
     8101 atctatcttt tagtattaat agtttaggta cggaagaatg ggataattct tttggaaaag
     8161 tatactcagg cttaatagaa gctggattac cttcaattat ggcaggtcat atccatatgc
     8221 cagcttattc aaaacatttt aatcctgatc tgaaagatga agatttactt cctgcaactt
     8281 tatcaaaaga attaatcact gacttactta gaggaaaact cggatttaat ggattagtag
     8341 ttactgatgc tagtcatatg ttaggaatga ctggggcaat gaaacgatca gaattattgc
     8401 ccacttctat tgcagctgga tgtgatctat ttctattttt taatgatcca gatgaagact
     8461 ttaattatat gatggaaggc tatcgtaaag gaattatcac tgatgatcgg ttattagatg
     8521 ccttaaaaag aattttaggg ttaaaagcta aattgggatt acacaaaaaa gcaaaagaac
     8581 aaattttatt acctaagcaa gaagctctgg aaaaaattgg tttaattgaa aataaatcaa
     8641 tttttaaaga ggtggctgac caagcaatta cattagttaa agataaacaa gatatttggc
     8701 ctattgtacc agaaaaatat aaaaggattt tactagtaga tgtacatggg gtaaaaggtg
     8761 ggtttggtga agtagttgct ggaaatagac ctcaagccat tgatttattg aaacagattt
     8821 tgtcagcaaa aggatttgaa gttgaaaaat acatttcacc tgaagaagaa ttgaaaggat
     8881 tatcgcaaga agaaaaa
//
LOCUS       DASJRQ010000104         8673 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJRQ010000104 DASJRQ010000000
VERSION     DASJRQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.105"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(271..2652)
                     /locus_tag="U4A80_002349"
     CDS             complement(271..2652)
                     /locus_tag="U4A80_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1975957.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2642..3289)
                     /gene="recU"
                     /locus_tag="U4A80_002350"
     CDS             complement(2642..3289)
                     /gene="recU"
                     /locus_tag="U4A80_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1975958.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3353..3892
                     /locus_tag="U4A80_002351"
     CDS             3353..3892
                     /locus_tag="U4A80_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1975959.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3982..4401
                     /gene="gpsB"
                     /locus_tag="U4A80_002352"
     CDS             3982..4401
                     /gene="gpsB"
                     /locus_tag="U4A80_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1975960.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4423..4790
                     /gene="rnpB"
                     /locus_tag="U4A80_002353"
     ncRNA           4423..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4A80_002353"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4999..6165
                     /locus_tag="U4A80_002354"
     CDS             4999..6165
                     /locus_tag="U4A80_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1975961.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6211..7707
                     /locus_tag="U4A80_002355"
     CDS             6211..7707
                     /locus_tag="U4A80_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1975962.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7788..8471
                     /locus_tag="U4A80_002356"
     CDS             7788..8471
                     /locus_tag="U4A80_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1975963.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2728 a         1572 c         1843 g         2530 t
ORIGIN      
        1 caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa
       61 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
      121 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      181 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      241 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      301 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      361 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      421 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      481 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      541 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      601 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      661 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      721 ggctacgtgt gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac
      781 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      841 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      901 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      961 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
     1021 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1081 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1141 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1201 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1261 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1321 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1381 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1441 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1501 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1561 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1621 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1681 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1741 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1801 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1861 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1921 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1981 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     2041 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2101 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2161 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2221 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2281 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2341 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2401 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2461 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2521 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2581 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2641 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2701 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2761 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2821 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2881 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2941 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     3001 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3061 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3121 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3181 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3241 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3301 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3361 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3421 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3481 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3541 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3601 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3661 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3721 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3781 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3841 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3901 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3961 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     4021 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4081 aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac
     4141 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4201 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4261 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4321 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4381 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4441 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4501 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4561 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4621 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4681 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4741 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4801 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4861 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4921 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4981 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     5041 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5101 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5161 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5221 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5281 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5341 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5401 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5461 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5521 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5581 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5641 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5701 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5761 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5821 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5881 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5941 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     6001 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6061 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6121 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6181 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6241 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6301 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6361 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6421 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6481 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6541 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6601 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6661 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6721 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6781 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6841 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6901 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6961 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     7021 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7081 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7141 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7201 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7261 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7321 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7381 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7441 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7501 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7561 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7621 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7681 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7741 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7801 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7861 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7921 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7981 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     8041 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8101 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8161 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8221 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8281 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8341 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8401 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8461 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8521 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8581 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8641 tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DASJRQ010000105         8424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJRQ010000105 DASJRQ010000000
VERSION     DASJRQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.106"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            471..2108
                     /locus_tag="U4A80_002357"
     CDS             471..2108
                     /locus_tag="U4A80_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975964.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="U4A80_002358"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="U4A80_002358"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1975965.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="U4A80_002359"
     CDS             complement(3184..3819)
                     /locus_tag="U4A80_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1975966.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="U4A80_002360"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="U4A80_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1975967.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="U4A80_002361"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="U4A80_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6417..>8424
                     /locus_tag="U4A80_002362"
     CDS             6417..>8424
                     /locus_tag="U4A80_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1975968.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DASJRQ010000106         8107 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJRQ010000106 DASJRQ010000000
VERSION     DASJRQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.107"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            49..1473
                     /locus_tag="U4A80_002363"
     CDS             49..1473
                     /locus_tag="U4A80_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1975969.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1654..2427)
                     /locus_tag="U4A80_002364"
     CDS             complement(1654..2427)
                     /locus_tag="U4A80_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1975970.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2424..3329)
                     /locus_tag="U4A80_002365"
     CDS             complement(2424..3329)
                     /locus_tag="U4A80_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1975971.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3483..3803)
                     /locus_tag="U4A80_002366"
     CDS             complement(3483..3803)
                     /locus_tag="U4A80_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1975972.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4277..4963
                     /locus_tag="U4A80_002367"
     CDS             4277..4963
                     /locus_tag="U4A80_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1975973.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4965..5909
                     /locus_tag="U4A80_002368"
     CDS             4965..5909
                     /locus_tag="U4A80_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1975974.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            6108..6752
                     /gene="pcp"
                     /locus_tag="U4A80_002369"
     CDS             6108..6752
                     /gene="pcp"
                     /locus_tag="U4A80_002369"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1975975.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            6831..>8107
                     /locus_tag="U4A80_002370"
     CDS             6831..>8107
                     /locus_tag="U4A80_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975976.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         2525 a         1442 c         1693 g         2447 t
ORIGIN      
        1 tttgttatac tttttgataa aagcggtttt attttggagg atgcttacat ggatacagag
       61 caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg ttcagcgatt
      121 ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg cccagctatt
      181 ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat caataatatc
      241 aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga aggttttggc
      301 cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt aggcaacgta
      361 gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt tgaaggtggg
      421 caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc atggctagtg
      481 aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg taaattagtg
      541 ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca attcattgat
      601 aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt ttccagtcaa
      661 gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg agcctctgtc
      721 gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct agggatattc
      781 ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat gacgatgaat
      841 cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat gatggttggt
      901 aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg tgtttggctc
      961 tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg acttttgcct
     1021 aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt attgacgacg
     1081 ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta taactttgct
     1141 tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt gtaccaaatg
     1201 aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt agcaagtgcc
     1261 tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt tcttgttctt
     1321 ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga gcctgtacaa
     1381 catatccact tagataagat tttacttata ggtgtgacga tggtcggagt gattgcaatg
     1441 gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat aaagagaggt
     1501 tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac aaatgttgtc
     1561 acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa taaggctgaa
     1621 cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct taaatacgag
     1681 atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca tttgattagt
     1741 agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt gcagattcaa
     1801 catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa taaggatacc
     1861 agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg cgccggaact
     1921 attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac cgatataact
     1981 acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa ccttctccgt
     2041 gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa tagctaaaag
     2101 gtataaataa acagaataaa taactaatgt gatccatttg cttactaata tttctcctcg
     2161 agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca tcgaaatgat
     2221 cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa aaagctgagc
     2281 agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg ctgttcctat
     2341 gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga tcaatttaaa
     2401 taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga atccagaatt
     2461 tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac atggatattt
     2521 tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt taaaagcttg
     2581 attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg agcagctaga
     2641 tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc agccattgta
     2701 cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat tttttcaagc
     2761 tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg
     2821 atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc atctaaaata
     2881 acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt cattcccaac
     2941 gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt cacgatagtg
     3001 tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata gcctgttaag
     3061 aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc tttatgacta
     3121 gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg gatcagtcct
     3181 aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa tccaacgatt
     3241 ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc aaagttcttg
     3301 gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat agatagtaca
     3361 agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct tattttttca
     3421 taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact tattccaagt
     3481 gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat ccccacaaag
     3541 aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat gaccgaacca
     3601 actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact tttgattgcc
     3661 tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga ataattgatt
     3721 ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa aattcctgct
     3781 atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa aatacacaaa
     3841 aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta tcctacccgg
     3901 tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc ggcaatctca
     3961 tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa aagaagatga
     4021 atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa aaaacagtaa
     4081 ctagacctta tttctatata ttatcgggca ataaattaaa aaaagcttta ccttacaata
     4141 agtaagataa tttttgtgat aaaaaaataa cgaaaaatat ttaaaaatag tgtctatttc
     4201 gtgagaaaac aactatattt tttatgtgtt ttgctttata attaaaaaaa tattgtcatt
     4261 ttatggagga aaatatatgg aaaattatct tagcctttta ggcgtcttga ttgttattct
     4321 cgggtttgca tttaaattag actcgatatt gatcgtaatg gttgctttag ttgttacagc
     4381 tttgactggc ggtcttggca ttcaaggaat gttagaactt ttaggtacaa gttttgtaaa
     4441 caatcgcggg atggcgattt ttatcatcat tatgttggca acaggaacgc ttgaaagaaa
     4501 tggtctgaaa gaatcagcgg cttccttgat caagcgattc aagaaagtat ctgctggtat
     4561 gattgtagat atttatggga tctttcgaat gatctttgcg gcttttaacg tgagttttgg
     4621 tggagttgct ggatttgttc gtccaatcat tttaccgatg gcattgggga ccatcgaatc
     4681 aaaagggtta gaagtccatc ctaagcatga agaagaacta aaaggtatgg cttcagctat
     4741 ggaaaatgtc tgctggttct ttggccaagt actattcatc ggaggagcag gggcgttgct
     4801 agtccaatca acactgaaag atctaggata tgacgtaaca ttgggacagc ttgctttagt
     4861 ggaagttcca gtggctatca cggctttagt tgtggcaagt gtctacttct atcttaaaga
     4921 aaagaaatta gcgaagaaat attatccagg aaaggggcaa taagatggac ttttttacaa
     4981 atcctgatat tttattaggc gataaattac tggagatcct ttatatcgtg atgggactcg
     5041 tattgattta cactggagta agaaatttaa cagataaaac caatcctcat cgttatgggt
     5101 cagccttttt ctggacggtc ttaggggtag ttattgcagg aggaagatgg cttccggcaa
     5161 tcgtgaacgg tggcttgatc tttgcgatga ccataccagc aattgctaag aaagtcagca
     5221 aaggagaaag ccggcttcca tcaaaagaat atatggaaaa aatgtcagac aaactaggaa
     5281 tgaaaatttt tattccagct ttgagtatcg gtgtctttgc gatcttgttt gctctattta
     5341 ccaatttagg tgctttagtc ggcgtgggcg tcggtgtttt tgcagccatg ctaatcttga
     5401 tgttcttttc tcgagataat cgaccaagta catttttaga tgatgcagca gatatgctgg
     5461 gcacagtagg gccgttaagc atgttgccaa tgcttttagc ttctctaggt gcggtattta
     5521 caagcgctgg agttgggaca gtgatttcca atgcagtagg tacgatcgtt ccagaaggaa
     5581 atgtgaacat cggcatcatc atttacgctc ttggcatggt gatatttacg gtaatcatgg
     5641 ggaatgcatt tgcggccatc accgtgatga cggttgggat cggtgcacca ttcgtattta
     5701 gtcatggagc gaatccagcg ttaataggaa tggttgcatt gacttgcggt ttttgtggga
     5761 ccctgctgac accgatggct gctaacttca atatcgtgcc tgtagcgatg ctggagatga
     5821 aagacaaata cggagtgatc aaaaaccagt tgtttattgc gttatttatg ctagttttcc
     5881 aaattgctta tatgattcta tttaaataat agaagagagg ggttgtgaca gaataattgt
     5941 cgcaacctct tctaacattg tagtggaaca ctattttttt ataagaatat attaagtttg
     6001 atctgttatg ttatttaaac tattgtttcc tttatttacg ctgttcaagg aaaatggtat
     6061 gataaaagat aaccatatgt taaatcaatc ggacggagga acaacagatg aaagtactag
     6121 taacaggttt tgatccattt ggtggggaca aagtgaatcc agcctatgaa gcagtaaaaa
     6181 aaatgccaga tgaaatcagc ggtgctgaaa tcatcaaagt ggaagtaccg accgtattcg
     6241 aaaaaagcag tcaagtagtc aaagaagcca tccaacagca ccaaccagat gtcgtgatct
     6301 gtgtcggaca agcaggtggg cggtcagcag tctcgttcga acgtgtcgca atcaatctag
     6361 cagaagcaag aatccctgat aatgaaggaa atcagccatt cgatacggct ttagaagaaa
     6421 acggtccagc cgcttacttt acctcattgc caatcaaagc aatgacaaaa aatgtacaag
     6481 atcatggtct accagcgtac atctcttata cagcaggaac atttgtctgc aatgatatca
     6541 tgtatcgttt gcttcatttg atcgaaacac aattcccggc aattcgaggt gggttcatcc
     6601 acgtgccatt ctcacccgat caagtgatcg atcgtccagt gggtacgcct tctatgtctt
     6661 tagaagacat tgcttcttct ttgacttatg cagttgaagc agcagtagaa aataaagaag
     6721 atatttccgg aaatgccgga acaactcact aatttagcaa gaaacactgg ctggttgcta
     6781 acgttatgtt agtagccagc tttttgtaat tagaaaggaa agaaacagcc gtggaagaat
     6841 ggcaatccgt ctttgaagaa tggtttccaa aagaaatcag caaaagctat cccatcaaaa
     6901 tttctaaaca atacacaagc agtcaacgtt gggagatcta tgcaaaatta acgaaaaaac
     6961 agcgggaact agtcgataag caccgccgat atctaatcag ctctcgtttt atggaagaac
     7021 attatttagc tgcaacagat tgggtgtttt ctgacttcaa gatcaatccg ttttttcgaa
     7081 caaaacgaag ccagcaaaaa ttgtattgtg agtgtggtag agaattaaaa gtgcaataca
     7141 tcgttaaatc gccaaaaacc ggaaagatcc taaaactagg catcaaccac tttgccgatc
     7201 acctgcatgt ctctccgaca gtggcagctt cgatccatca gggaatgaca aaagttgatc
     7261 tggccttaga cgaattatta tggcttaagc agaagaatat cgattttcct gaaggactat
     7321 ggcaaaaata ctgttttgtc ttgtaccaaa atcgtcggat gaaacagcca tatttaccgg
     7381 atataaaatt agctcagcgg ctcgcagaat ttcgccaagt agaaatgccg atttacatag
     7441 cggattatca agcattggaa aatgaaatca agaaaataag tgaacacatc aatgggcagt
     7501 caaaaaagcg acagataaaa aaagaactat ttgacgactt tgcagaagaa ttagtaaagg
     7561 atgtagaaga attcttgatc aactatcgcg catttttacg taaagattgg caatccatcg
     7621 tatatgaaga agtgccagta catcctaatg cttattttga aacatttatt tccgtgttga
     7681 gaaagacaaa acgtcaacga acaccagaag tcactgctca gatggaatat tttgcgaaaa
     7741 accaacgatt catccaacca aaaatctatc tatttatttg gaagcaatat tgtcgctatg
     7801 gttttacaga agggttcttc gacagcattc ctcggatagt cagaaatggt tttttaaaag
     7861 tattgcgtaa agaacgagaa gctatacaat ctgctgataa gaaagaccgt acagtctcaa
     7921 aagaaaaatg gcaactagta gtgaaagata tccaatctgg aaatgtgcaa gagacaatcg
     7981 acaaatggaa aggaaaacat tatcgtttta ctgaagctca gaaacaagcg ttggaatatt
     8041 atcagaagtt agaagaatca ctgcgtttca atgatgaggc aagaaagtat ctgaaggaat
     8101 tactata
//
LOCUS       DASJRQ010000107         8097 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJRQ010000107 DASJRQ010000000
VERSION     DASJRQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.108"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..245)
                     /locus_tag="U4A80_002371"
     CDS             complement(<1..245)
                     /locus_tag="U4A80_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975977.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELT"
     gene            complement(453..1379)
                     /gene="glsA"
                     /locus_tag="U4A80_002372"
     CDS             complement(453..1379)
                     /gene="glsA"
                     /locus_tag="U4A80_002372"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1975978.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1402..2613)
                     /locus_tag="U4A80_002373"
                     /pseudo
     CDS             complement(1402..2613)
                     /locus_tag="U4A80_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2648..4261)
                     /locus_tag="U4A80_002374"
     CDS             complement(2648..4261)
                     /locus_tag="U4A80_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1975979.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4373..4582)
                     /locus_tag="U4A80_002375"
     CDS             complement(4373..4582)
                     /locus_tag="U4A80_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HEN1975980.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5571..6785)
                     /locus_tag="U4A80_002376"
     CDS             complement(5571..6785)
                     /locus_tag="U4A80_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1975981.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6913..7383)
                     /locus_tag="U4A80_002377"
     CDS             complement(6913..7383)
                     /locus_tag="U4A80_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1975982.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(7380..7622)
                     /locus_tag="U4A80_002378"
     CDS             complement(7380..7622)
                     /locus_tag="U4A80_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HEN1975983.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
BASE COUNT         2302 a         1724 c         1301 g         2770 t
ORIGIN      
        1 tgtgtgagct cagaaagagc aataccatgt ttgaatacga gtttatactt atgcttctct
       61 cttacttgtt cttttataca atattctaga cactcggcta gactttctag atcgtgaagg
      121 atcgttggtg tagtcaccct tagagaaata gctgtttgtt cgatgtctat ttcttggaat
      181 tcaaataatt gttcacacag atacactttt ctaaatatat ttcgctcgat gtaattttcc
      241 agcattcctg atacacgcct ccagtttttc ttatgatttt atttaatcat atctgtaaat
      301 cgtatttttg tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac
      361 tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt
      421 tggactttta ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct
      481 ttgactacat ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc
      541 gggctgaaga cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca
      601 ccgctttttg caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca
      661 gcagttgtca taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca
      721 ttccaaggag caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg
      781 gtgacattga gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg
      841 atcatttgat tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat
      901 tcagaatgat agatttcttc attcaatgtg atattaggat catcacagat ctctttcata
      961 aatgacaaga ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt
     1021 gcaatcgctc ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata
     1081 ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt
     1141 tctattccgt tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg
     1201 gcaaagcgca catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc
     1261 gccataccta attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt
     1321 cctgtttgat aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt
     1381 tgaataacca gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta
     1441 gatcgtaaat ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat
     1501 cccatgctgt ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag
     1561 tgaagtcaaa agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg
     1621 ataggctctt tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat
     1681 aaagtacaac atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt
     1741 attccccccg ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc
     1801 gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa
     1861 tccttcttct gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc
     1921 tccgatgaca cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg
     1981 atcaatgtgt cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc
     2041 atgttgaggt actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag
     2101 aataaccaat acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc
     2161 atgactggca gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag
     2221 cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg
     2281 agcgcctgaa gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat
     2341 accagctaca aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga
     2401 gagtaaaata gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg
     2461 tatagcacca atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa
     2521 gtagccaact gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc
     2581 caatgtgcga ctgacccaag tatagacacc gccggtttcc cttagttaaa aatattactc
     2641 ttgttgatta ctcaactctt ccaataaatc ggtaatatat ctcaatttcc tgtattcttt
     2701 cgttatcctc attcgttgtt gcttcatgta tgaggatttt ttctatcatg gcatttaata
     2761 atggtgctgt cagttccttt ggtacggaat attccttgat taactcaatc cacttttcag
     2821 cacctatcat atcctgttcc attttactta gttcttctct taggcttgtt atctgctgtt
     2881 ccagttctat ctgttctttt tggtacttgc tggacaacat cacaaaattt cgctctgtta
     2941 tcttctcaca ggctctatct tcatacattt tcgcaaataa acggtcaatc tcattttgtc
     3001 tgttctcggc tttcttcaat gtacttgcct ttttcttctt ttcccgtatt ctctctgcat
     3061 tgccagcttt ctgtatcttg ttcaatacct tttcttcgtc ctgctgtact gccttagccc
     3121 aatactgcaa gcgttccaat acggcttgat acagcacatc ataacgaatg tagtgcatag
     3181 aacaattacc tttgccaaac tgcccgtagt aactgcaagc ataataactg taaggcgttt
     3241 tatttgtctt attcgttcca aatctcatag accaaccaca atccgcacac ttgacaagcc
     3301 ctgcaaaaat cggcgttgcc ttttcctttg tctgtctgcg tcttgatttt atctgctcct
     3361 gcacacgata gaacacttcc ttgtcaataa tcggctcgtg agtgttttct actcgaaacc
     3421 attcactttc gggcttacgc actttctttt tgctcttgaa cgataccgta gactgcatat
     3481 tgtgaacact gtttccgata taaacctcac ttttcaatat cgccttgaca tgagcaatcg
     3541 tccactcata acgcttactt tcgggttttc cctcaaagat atgtgcaaaa gtaccgtatc
     3601 ttgtaaaatt tagccatgac gctgtcggaa ctttttctgc tcttaactgc tttgtgattt
     3661 ttgcacttcc gtaaccttga taggctaggg aaaagatttt ttcaataatc cattttgttt
     3721 catcatcaac caacagtttt cctttgatgt cagggtgttt cttatatcct aacggagcat
     3781 aagcaccgta atgagccccc tccgcaaact ttgtcttaaa tgctgatttg actttacggc
     3841 ttgtatctct tgccacccat tcattgatga tgttcttaaa tggtgcaatc tcactttcgc
     3901 ctttttcgct gtctacaccg tcatcaatcg ctatgtagcg gacattatga ctagggaaat
     3961 acaattctgt atattgtcct gtcataatat aatttctgcc aagacgggaa aggtctttcg
     4021 ttacaacaca gttgattttt cctgcctcta tatcctcaat cattctttga aaactcggtc
     4081 tgtcaaaatt tgttcccgac cagccgtcat caatatattc atcaatgaca ttcaaatgat
     4141 gttcttttgc atataaacgc aacatacttc tctgtgtggt aatgctggaa ctttcgccct
     4201 gtaattcatc atctcggctt aacctaagat aaagtgcagt attgtaaatc tgttgtttca
     4261 ttgtcagtta tcctccttta taactaaccc gtgtttacaa tacctgcttg caagtaaagt
     4321 ataacacggg tactttttgt cattcaatct atttcttaca actgcaccga ttttatgcga
     4381 ttgtctgctt tgctttttcc agcatgacat cagttagaag ttcttccatt gttttgccct
     4441 tttcggaaaa atgctctttt actacaatct ttgtctttcc tctgttgcca atgccacact
     4501 tgccattttt cttttgaatt gtttttacag cattatcaat aacacatacc ccctttctgt
     4561 aaaatctttc taagcaattc aacggctttt gtcatagccc acaggagttc cacctctgca
     4621 tagttcttat gtagccgtac ccattgcgtg cgacgctctt aatgctcaag ctgtggctgt
     4681 acgggagtat cattatctga cagtacaggt catggcgata aaagaggaca aatcttttac
     4741 aagaacacaa ataggttttc gctcgctctt gcaggggaaa ggtcgtggcg tatttgtttc
     4801 aacggctagc tgtctgtcaa acgtattgaa tagctttatt cagttgtcaa agaacgattg
     4861 aaagaaaaaa atcgtctttc ctatatatcg cgttggaaaa gaggagaaac gtaaccaaaa
     4921 tccaaaactt tttccattat tagtacaaat ggaacggttc attttggcaa tcaacggttg
     4981 attttctcct ttttcactac tatctgtaca gcaaacgtcc atttgctaag tttaaatgga
     5041 aaaattttaa cttcttttca tgcaccatat agggatagaa aatgtcattt tgctaagttg
     5101 atgatgaaaa aagaatattt tccttttcac accctaacta cccaaaccat accgttactg
     5161 ccaacaatag aatgaaatat tttctttgca ccatataggg aacggaaacc ttaaattgtt
     5221 aagttgagaa caaagtattt tcatcttttc acatcaacac tcaactgcta atgcctgttt
     5281 gaatacaaaa tgatgatgtt tttcctcttt tcacagaaga ctgtccacta atcacgaatt
     5341 tccgtcattt tttcaaaact tttttcttct ttcacttcat atagggacag caagccccga
     5401 tttaatgacc tattttcaaa aaaagtttag atttcctttt ctcgcaccat atctgtacgc
     5461 aagtagccat tttgttgcag atttaataaa aaatttattt ttcttctttc acttcacata
     5521 gggaatgaaa acagctcttt gctgactaaa tctgaaattt ttcaattaca ttaaaaaata
     5581 ccgtcattct cttgtggaac agcggtatct atacgttctt ctatggttga tttctctaat
     5641 tgtaataaat gtttatgctt gtcttttagt tctgcctgct caatcatatc tttcagtatc
     5701 gttgtacgat tttctctgtc taatgcctgt accattttta tggttggtgc gacttgtctt
     5761 tgtagccaat gcaacgcttt ttgtaaagtg taaggctctg gctttgtggt taagcgtatc
     5821 ggctctctgt tttcccctac aaaccatgcc caatcatcat ttgttatcca ttgacttttt
     5881 ggcttgtcgt cctctctgtc aacaaagcgg acataatgat tgatgataga aaaagcggtg
     5941 cgttcagcgt cccgataggt cagtaaatct acaaccgcat aataggctcg ttcgttcttc
     6001 atgcgtattt caaaacggtt cttaatctcc gtatcttcaa tttctgtgcc attcttgaca
     6061 tactgctcat aatttttctg ataagcacac atatataatt cgctacttgt tgaaccgaga
     6121 tataaggttt ctcctgtatt ctttggtaaa tcgctaccgc atttttctgt accgctgtaa
     6181 tctttctgca tacggaagta ggacatacat tcgccagcct tgtatttttc ctttaacttt
     6241 ggaatatcca atattcctgc tttatcattg atagccaaat caagccgttt catcacacca
     6301 ccagccgtca tacaatccag catgaagtca taccatgagc gttcctgtgc cagcagataa
     6361 cattccattt gccgacagcc tttgcctttc agttccaata aaacacctaa atgctcctgc
     6421 atggaacaca tgatattgat gtcgccaagt acatatttgc tttcatatcc gtattttcca
     6481 taatcttcat aaagcatgta attagatttc agttgtaata catcacggat aatcgccaat
     6541 gcgtccgtag taggaaagcg gacacgaaag taatcaatca ataagaatag tggttcttgc
     6601 ggattgaaac gctcaatctg tttttctatg atttctctta attcgtcatt caatggctgt
     6661 tttccttttt caatgcggtt gtaatattca cggctgatac cacaggcaac agcaagcctt
     6721 gtttgtgtta ctccgtattc ctcacgcttt tgtcttagtt cttcaataaa gtttttatca
     6781 ttcattcttc aattcctcca atcaaaaaag gcaactacca taattgataa ttgcctgtca
     6841 ttaaaaatct gtatagaaaa agcagtcaat cctaagacta actgctttgt gtgtatggat
     6901 ataaaattgc ttctaatctt gaataggtat ccaaatttca ataactgaat tatcactatt
     6961 ccaatgaaaa cgataatcgt atttttcaaa gtgtaggata atatctttta taggaatata
     7021 cgatgtacta ggaataattt cttcataaat cttgccataa gtttcatata ttttatccat
     7081 agcaccaata tgctccacaa ccaaatattt ataagacggt atttctttat acagaaagcc
     7141 ctctggggta atggttcttt ggggaataga acagaaataa taaagttttc catttcttct
     7201 ttccataaaa gcatatttta cccaatttcc tgattgtgaa agatacgctt ttttcaaact
     7261 actgttaaac tttctccaaa actgtgtact aatctgaata ttcttttttt gataattagt
     7321 cagttctact tcctgcccaa taactgcaaa tgcgtttaat tcctttatag aaaaatttat
     7381 cattttacct tgtgagcagt gataacggta taactgtacg catttaccgt tataatacaa
     7441 ttatcaatcg ttatatacca gttttttcct tttcttgtga tttccgcatt agaaaggcta
     7501 attttttctt tgcaccattc aattacattt tctacattca aagataagtt ctttttaatt
     7561 ctaataacgc caagttcagt tgtgtgaagt cttcctaagt tttccagtaa ttcattttcc
     7621 atattttgat acctcatttt tcagcaaatt tatttgtatg ttttataccc caattataac
     7681 attctcatat cagcactaca ataaaaataa ttaagcacca ataatcttat tgaacatctc
     7741 caaccgtaaa tctttcacac catcagtgat aaacactgag ctgacagcaa ttacgacaac
     7801 taccataatt gcctgcactt aaatttctgc aaaagaaaac gacaatcaat ttcttatttt
     7861 ttgaaatcaa ctgtctttct catatttact attcaatttt catagtgata taggtacaat
     7921 ttatatcact actgtaaaaa tggggttttg ccttataaat acagcgtttt ccgcttgtta
     7981 gtgtatagtg tttcttgttt tttgtgcccc ccttgttaga taacgggggc tttgaacggc
     8041 tcgcaagctc gccgaaccgc cgaagcgaac cgcccccttg caagcatggc ggaaaaa
//
LOCUS       DASJRQ010000108         8084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJRQ010000108 DASJRQ010000000
VERSION     DASJRQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.109"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            289..696
                     /locus_tag="U4A80_002379"
     CDS             289..696
                     /locus_tag="U4A80_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975984.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="U4A80_002380"
     CDS             710..1111
                     /locus_tag="U4A80_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975985.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="U4A80_002381"
     CDS             1113..1484
                     /locus_tag="U4A80_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975986.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1520..1825)
                     /locus_tag="U4A80_002382"
     CDS             complement(1520..1825)
                     /locus_tag="U4A80_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975987.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2526..3737)
                     /locus_tag="U4A80_002383"
     CDS             complement(2526..3737)
                     /locus_tag="U4A80_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1975988.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(3855..4490)
                     /locus_tag="U4A80_002384"
     CDS             complement(3855..4490)
                     /locus_tag="U4A80_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN1975989.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(4573..4995)
                     /locus_tag="U4A80_002385"
     CDS             complement(4573..4995)
                     /locus_tag="U4A80_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1975990.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(5013..5333)
                     /locus_tag="U4A80_002386"
     CDS             complement(5013..5333)
                     /locus_tag="U4A80_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1975991.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5626..5817
                     /locus_tag="U4A80_002387"
     CDS             5626..5817
                     /locus_tag="U4A80_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975992.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            5831..6082
                     /locus_tag="U4A80_002388"
     CDS             5831..6082
                     /locus_tag="U4A80_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1975993.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            6058..6237
                     /locus_tag="U4A80_002389"
     CDS             6058..6237
                     /locus_tag="U4A80_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975994.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6280..6750)
                     /locus_tag="U4A80_002390"
     CDS             complement(6280..6750)
                     /locus_tag="U4A80_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975995.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            6837..7535
                     /locus_tag="U4A80_002391"
     CDS             6837..7535
                     /locus_tag="U4A80_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1975996.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            7713..8054
                     /locus_tag="U4A80_002392"
     CDS             7713..8054
                     /locus_tag="U4A80_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1975997.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
BASE COUNT         2718 a         1265 c         1392 g         2709 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctttgt atctttctgg atcaacgaaa gtatacttta
     1561 tatagtcata acgccgatga tcgctacgtg cgtccggcac atcagtcacg atatcaaaca
     1621 aaaagtatac acccttcttc attctagttt tcgcagcagg aattttgaaa tagttcttat
     1681 tagaatagta gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat
     1741 cccacacctt ataaaaatct ttgacaaatt tttctgatgg atcgtattta aaccaaagca
     1801 gatgttcctc tagctcgatt gtcatgtttt tcacctcaac gaaagtatac gaactaatgt
     1861 ttgttttgta aaggataaaa atagggtagc aaccggtacc caaactagta cccatttatc
     1921 aaaaacgacg aaatttatgt aaatagaaaa ataagaataa acattgatgt atcaacattt
     1981 atatatcttt agaacaaatg aaaagggtgg ttctcataag aaaaacatgt tcgcacttct
     2041 aggcaaacca ggatttgaag accttgctaa agaattgaac gaacgcctgt aaaataagga
     2101 tttgtcgcta gatttaaaaa atcagtgata gaaacatgtt tgctttaaaa tgaaactagt
     2161 gatagtttta gtgatagtga acttaaaaaa agcactcaat ttgagtgctt ttttattgtt
     2221 attaaaaatt aaaaaactaa aatacatacc cgaatttcat acccgaaata taaaaaatgt
     2281 catcacgtac atctttttcg aaactaccga agtagccaaa gatgtactga tgacgtgggt
     2341 ttggcaccca atgggccgtg aggggctcga acccgcgacc cgctgattaa gagttcgtta
     2401 tttaatggta ttttaaatga agataagcga atttacagca tttttaaatg cttatatacc
     2461 gcaaattatc ccaagttttt atatatccta cccgagttta tacccgaaaa tataattttt
     2521 tagtgttatt ttatatcttt ttttaatagt ttttcaaacg ctgtagttgc tgtatctttt
     2581 tctttgcgta agacatggcc ataaacattc attgtagtat taaaatctcg gtgtcctaat
     2641 cgtttttgaa gctcttttgg agtcatatta ggatcgctaa gcaaataggt agctgatgta
     2701 tgtctaatgt cgtgaaaacg aatgtgacgc aaattgtgac gtttggtgaa tcgtttgaac
     2761 ttcttataca gataacttcc acgtggtaaa gttccgtctt gttttgtatc aaatataaaa
     2821 tagtgtctta atttctttat atttaatttc caacgaattt ttttattctc tttgatcaat
     2881 tcatctaaca tatcaaatag ataagcagga ccggttacta ttttttcatc atcatcattt
     2941 ttcaaacctg gcagcaaccg aatatctgat tttccatcca cttctgaaat ccgctgatga
     3001 aatcttattt cttgtttgtc aaagtcaata tctttttctt ctaaagctgc aatttctcct
     3061 tgtcttgctc ctgtaataaa tgctagaaca attaaagttt taatttctat gctttcctca
     3121 aatgctgctt ttagcattgc atgaatttct tcttccgaat aaggttcttc gatattcttt
     3181 tttacgcctt tttcttttgg tatttttaca ttttccacag gattttcttt aattgctccg
     3241 tactcatttt tagccatcaa aaataagttg ttaacggcgt ataacatacg aagctttgta
     3301 tttcttgata aaggtttatc agagtttctt ccaggatctt ttgtattgat tctttgagca
     3361 ttaacaacaa ttttctttat catgtatggt tttatatcga ttagagggat aaaatcaaat
     3421 ttatcaagaa atctattctc gatcattcgc ttatagttat gataagactt tggctctaaa
     3481 tttttttcgg cctctgaaag ccatattttt ctataaaatt caccaaaagt tatattgaat
     3541 aaatccaacg aatcatcggt cattccttcg aattgctcta accagatgtt caattcttta
     3601 tatgccgctc ttttactatt cgcttttata gttttgcttt ttctttttgc cgagccatca
     3661 ggtttgtacc ctaatacagc tcttagtcta tatgtttcat tgtctacttg ttctaagtat
     3721 cctgttactt ctgacataat tgaatcagct ctctttctct gatacaatag gtactataaa
     3781 gaagcctatt gtgtaggttt gttttttctt agaacacgct cgctttggtc ggtggggcgt
     3841 gttttttata ctaattagtt atgaagagca attgaaactg tctctgttcc aaagtaacct
     3901 gtttgtgctt gcaatacatt attttgtgca tttgcttggg cttcagagat atcaaaaact
     3961 attttaccag tttgttgcat atcaggattt aagctttcca taaagaaaga gttagtgata
     4021 ttaccatttt catcttgatt tgccgacata gatgcagccg aatcagcttc gaaagttttt
     4081 ccatctgctt ttaatttgaa gaatgagcta tcaacagtca cagccttatc acctgcattt
     4141 tttacagata agtcaactac taagaaagtg tcttttgcgt tagtaggaag tacagatggg
     4201 ccaacttgtt ttactacttc aacggagttt actttgtatt ccattttccc tacagcaacg
     4261 tcatcaccga ttttataagt cttttcttct tttgttgtag tttcttttgt agtttcttta
     4321 ttagtacttg ttgatgttgc agtagctgaa tctttgtttt caccgccact tagagcacca
     4381 ccaataccaa aaaacaaaag tactattaat acccaaaacc atacccgttt ataaaacggt
     4441 tttttaacct tatacatttt cccatcttga cccataactt tttttgccat ttaaatatcc
     4501 ctcacttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc cgtgttgcag
     4561 cacgggcttt ttttactgtg cataagagta ttttttcttg aaatatgact ggcaaacatt
     4621 aaaacattct gttcttaact tattattgat agagtagaat tccatgaaat tttctaattt
     4681 gaactgagat tcatcagtca attcgttctc aataaagata tttagtaaaa tcataattgc
     4741 gattctatca gcttcagctt cgaattttga atgaaaagtt gtagagttat cgtacagtac
     4801 tgaatattca aaatgtgaag caatgaaatg accgagctca tgggctaaat gaaaagcttc
     4861 agaactgtct tcgtgtagtt tttcattcaa aaatactatt cttggttttg gataataaaa
     4921 acctggttct tccatttcca tatagattaa ctttaaatta tactcactca gcatttcttt
     4981 caactttaaa tacatacaac ccatcactcc aactattcat tttcctctaa agctttagca
     5041 attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaataca
     5101 gtatcatctt ctgataaatc cacatgttta ggcgtttctt tttcttctct acctagaaga
     5161 tagtctacag agacattgaa atagtcagct accttttgca aatctcttgc tttgggttct
     5221 gttgttttcc atcgatagaa aagattttcg ctaaatccta attctaaagc gacttctttc
     5281 atgcttttat ctctttggct agctaatgat tttatccttt caaacagtgt cataataatg
     5341 ttctccaatc ctaaacaaat aaaagtacac aaaatgtaaa ttatactgtt gacataagta
     5401 taagtttgta ttattatttg tttgtaagtt aatttgatag aaaaaaagca aactaaaaat
     5461 acaccttata gcattaagtt tggcgaccga ttgcataata aagggtttgt tgtaggctta
     5521 tttaactatg tttatatagt atacgattgt atactattag tcaatagttt atacgttttt
     5581 tctatcattt tgacttacaa atctatatgt aaggggagtg aatagatgtc aaatctagat
     5641 aacggccgtg ctgcaatcaa gaagtttatg cgagaaaacg gagtaactat ggaagatttg
     5701 gctactgcat atggatacac acgtgttcga atgcagcaaa ttattgatgg ccattggagt
     5761 ggaccaaaag ctaataaaac tatcttagaa atcatccgtg attacagaat tcgataggag
     5821 gaattaatca atggaacaac tagctttagt taacctctca gccctcaaag cttctttggc
     5881 agaatctgag attgcaaatg aagtgtggga tacaaagcaa gctgcggaat atttgaaaac
     5941 cacgacacgc acgctaacta aagatgccga atctggaaag attccagcag cgaaagttgg
     6001 cagagagtgg aggtttagta gtatcgcttt gtatcagtat ctaaaaggag gaaaaacatg
     6061 ggcaaattca acagagcact agtattcagc gcaccgctaa tcatctacgc tttagggctt
     6121 tggggaaata gacaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
     6181 cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa
     6241 tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa
     6301 tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt
     6361 tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac
     6421 ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc
     6481 agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt
     6541 agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg
     6601 ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga
     6661 agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa
     6721 cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt
     6781 caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc
     6841 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
     6901 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
     6961 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
     7021 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
     7081 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
     7141 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
     7201 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
     7261 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
     7321 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
     7381 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
     7441 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
     7501 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
     7561 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
     7621 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
     7681 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
     7741 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
     7801 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
     7861 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
     7921 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
     7981 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
     8041 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aact
//
LOCUS       DASJRQ010000109         7835 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJRQ010000109 DASJRQ010000000
VERSION     DASJRQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.111"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1094)
                     /locus_tag="U4A80_002393"
     CDS             complement(<1..1094)
                     /locus_tag="U4A80_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1975998.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFEL"
     gene            complement(1094..2230)
                     /locus_tag="U4A80_002394"
     CDS             complement(1094..2230)
                     /locus_tag="U4A80_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1975999.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2262..3713)
                     /locus_tag="U4A80_002395"
     CDS             complement(2262..3713)
                     /locus_tag="U4A80_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1976000.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3757..4521)
                     /locus_tag="U4A80_002396"
     CDS             complement(3757..4521)
                     /locus_tag="U4A80_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1976001.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4549..5247)
                     /locus_tag="U4A80_002397"
     CDS             complement(4549..5247)
                     /locus_tag="U4A80_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1976002.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5259..6038)
                     /locus_tag="U4A80_002398"
     CDS             complement(5259..6038)
                     /locus_tag="U4A80_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1976003.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6054..6998)
                     /locus_tag="U4A80_002399"
     CDS             complement(6054..6998)
                     /locus_tag="U4A80_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1976004.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7044..7823)
                     /locus_tag="U4A80_002400"
     CDS             complement(7044..7823)
                     /locus_tag="U4A80_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976005.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2183 a         1669 c         1198 g         2785 t
ORIGIN      
        1 attaattcaa ataactcata atattttgat tctaagattt tgtatgctgt ttgaatatta
       61 taatctgcct ttattaaaat cgaacgttct gaatctcgat gagagatatc tacatttgca
      121 atctctctcg cccattcttt tgccgattga tttaaactta tgaaattggt cttatcactg
      181 aaagcaactt ctgtaggaac tttctccgaa aagaaacacg gtaaatctgc aaattgagct
      241 tcaataccca ctaacggaac cccttcaaat aatgatggca acattaatac gtcaaatgct
      301 tgataaaatt tttctatatc atttctattt cctaaaaatt taatatcatc atctatgcca
      361 cttttctttg cataattttt tatttcttct tccatatcac cagttccaat aattaataat
      421 acggaattat tcttaatagc gtgaaattct ttaaaaattt caatcaaaaa acgatgattt
      481 ttagaatatg aaattcttcc aatatgtccc attacaaacg tctgacttga aattcctaat
      541 ttcattcgta catcatttct aatactttca tcataataaa attttttagc atccactgca
      601 tttggaatca aagtgaattt atcatttcga aacaaaaaat cacctgctac ttttccacat
      661 gaaaattcat gtgtcaaaac agacgttaac ttacatcgat aatattgctt taaaagatat
      721 ttaaaatctc tatctatcga tgtattatgg ctatgtgcta tccgtatcgg aatcccagct
      781 ttttttgcag cttttaaagg taaataactc atcgcatcaa tatgagaatg aacaatctca
      841 tattctggat gatccttaaa aaattgttcc atccatttaa aatatttagg ataattttgt
      901 ggataaagtc tcggtgcata atagaccttt cctccaagac ttattatttc ctcatcataa
      961 tcacttttat atggacgatg tgtcaaaaaa tcaaactgaa ttttagttcg atctatattc
     1021 ctataatagt tcattagcat cgtctccaac cctgcacgat gcatatcatt cacacactgt
     1081 aatattcgaa tcattatata tttcccctat acaaatttaa tgtttcttgt acgattttat
     1141 cccaattata tttactacaa ataaactcag tagcttgatt cttcaaaact ttaaccattt
     1201 ccgattgatt gcatgcctct tctaatctta tctttaaatc acttacatcc gatttcttaa
     1261 aaatcatagc cttgtcttct actacttccg tacactctga gatatttgaa actatacaac
     1321 agttcccata actcattgct tctaacaaac ttaatggcat tccttctaaa tcacttggaa
     1381 gtgtatagat atacgcatta ctatataatt cttctaattc ctgtccttgt acaaatccag
     1441 taaatataat tcgctcatca ccttttgcta attcctttag ttcatttgca aattcatctg
     1501 tatcagagct tcctccggct attataagtt ttttatctgt ctgaacatct ttaaatgcct
     1561 caattagata ccgaattcct ttttcgggaa ccaatcgccc caaaaacaaa atataagaat
     1621 cttttgttaa tccatacttt tctgtaatca atcctgctga ttttatttca atttttttaa
     1681 cgccatttgg tataaacctt gtttttcttc catatatatc ttcaaaataa ttttggacgc
     1741 tttcactaag tacaattatt tcatctgcaa attttacagc acacttttcc cctaacatga
     1801 tgtatgtact agcaagcttg ttccattttt ctctttgatg atctagacca tgaattgttg
     1861 caacacatct tttcccaaaa agttttggca accacaacat agcacatggt ccttctgcat
     1921 gaaaatgaac aatatcatat tttccgaatg ctgctctaat tccaccaaat actgaagaac
     1981 tcattgctgc aattcctttt atatttaatg taaaaatagt ctttaatttt acacctttat
     2041 attctttatt tttatgctgg tcaaattcct ttccgctaac atgatgacca cttcgattat
     2101 agcaagtcac ttcatgccca agtgcaacca ttcgcgttgc caattcttct accactatct
     2161 caattccacc ttctcttgat ggaattcttt tatgacctag catacagatt tttaattttt
     2221 ttcttgccat cacgttttac tccttctata gtatctttat cttacatgga accgtttcta
     2281 tgaagcacag ttttgactgt cctaaacaaa attctaaaat ctaatcccat actccaattc
     2341 ctgatatatt ctgcatcaag ttttacaacc tcttcgaagt ctgtgatatc acttctacca
     2401 ctcacctgcc acattccagt aatcccaggt ttcatcgcaa ttctcgcacg atgatgaaaa
     2461 tcatacttct catattctga cttcaagcaa ggtctaaacc ccaccaggct catttttccc
     2521 attaggacat tataaaattg tggaaactca tccaatgatg tagatcgaat aaattgtcca
     2581 attcctgtct tctttgttcc atcaggaagt attttattgc caatgattct tgggtcaaaa
     2641 tccatcttaa acatgagatt attactcatc ttattttctt tcatcaactc tgctttgcgt
     2701 tcttctgcat ccatatacat actacggaac ttatacatct taaatgtctt accattttga
     2761 ccaatacgtt cttgtgcaaa gaaaatcggc cctggtgatt tgatgtaaat aactggtgct
     2821 acgaagataa aaataacacc tgttgcaata catccaataa ttccagcaag aatgtcaatc
     2881 gttctcttta taaatgcttg cctcatcgtc ataacattaa tactagatgt aataacagta
     2941 taatcaccaa ctcggccaat aaattgtttt ccacgtgtac tatcagacac ttttgtaaga
     3001 ttcatatgta cagcaagtcc catttccgaa catctggcga ttaattcttt gggatatgga
     3061 acactttcgt caacattgat gaatacttca tctacccaat tctgtaaaag atattccgct
     3121 gcattatcag catttgcaac tactgggaca cctccgatgg tagttccata catatctctg
     3181 tctactacaa tcacaccact aagtttatac atgccataat tcttctcttt aagattacga
     3241 actacatttt ttgcaatctt ttgagatgtt acaataacta acgatacatc ttcaccgttt
     3301 ttcatgcggt gatgcaacaa ccctttccaa agtatacgaa ttatatatgt taataatcca
     3361 tacaccaccc ccattataaa taaagaaact ctggaataat catttccatc ctgcattgta
     3421 ataaggtata aactgcacac aagtaacagc ataaaagatt gctttataga agctacagcc
     3481 tctttataat atcctctttt taaaacattt ttatatgcct caaataaaaa tataatcgta
     3541 agattctcaa gttctataaa aattgccatg tggcgataaa taggaatcac atacggattc
     3601 cagtcaccct gtcgcattac atatgatata taaaaagcaa gctggacaca aaataagtct
     3661 aataaaataa aatcaatatg tttagaccaa ccacttgagt tttttctata catctctcac
     3721 tctctcctca tttccttttt atctacgaat aaagaactaa aataaaccaa acttcttctt
     3781 ttttacctct tcatagattg gataattgat ctcatccccg ttgatcagat cctttgcgac
     3841 ttgtttcatc tgtactactt tttccttacc aaactccttg gcaatctgct catagcactc
     3901 ttttaagtaa aaggtgcgct tagagacccc gtgtgcatca gaagcagcca tctgcacgag
     3961 attatgtttg accattgttt tggctgtttt ctggatatct ttgccaaata caccaacata
     4021 acttggcgca gtaagttggg ccaaacatcc catatctagg aacggaagca aatgatttgg
     4081 gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtatct ttcctagttc
     4141 taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat
     4201 cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat
     4261 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt
     4321 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     4381 ttttccatta ttgtggtgtg gtgtgcataa aatatgcgta atgccttgct gaatcgcttt
     4441 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
     4501 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta
     4561 gtaataacca gcatctttgc tgtgttctgc tccgttgtat acgacaccta agactcgtgc
     4621 ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg acgtattttc
     4681 ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
     4741 aacgactggc ggcatgtcga agatcactac atcaaatagt tgtcttgctt ctgctaaaat
     4801 ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag gtccacttgg
     4861 cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac ttcctgatga
     4921 actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat agatgacggg
     4981 cttacgtaag tctgcatcga ctagcagcac ccgttgacca gatttggcaa aaacaacggc
     5041 aatgtttgct gcagtggtcg atttcccttc tccaggtcct gatgaagtca caacaatcgt
     5101 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccgaatcg tacgatattg
     5161 ttcagcgatt ggtgaagagg gattggtgag tgtgaccaaa ctgactgcat gagtttgggc
     5221 tgctttttgt ttctgtgttc gtgccatttt ctttcctcct agactcttga gcggcttctg
     5281 cgtgatggcg ctgttttatc gtttgtagct tttggttgtg tctgagctgg tcctttttta
     5341 cggataccag cattcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
     5401 cctaatgtat ctgtcacata tttttcatct ttcactgtcc gatcgagtaa ttccaataag
     5461 aatgccagac ctacacctac catcattcct aacattaagc cagtcattaa attcaattta
     5521 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaatgatcga gattttatca
     5581 acattcatca catcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt
     5641 tgagcagcca ctgcattcgt acttattgcc tgaatcgaga acatttgtga attttctgcc
     5701 tgattaactg agatggcatc tttgatttgt tcagcagtca ttttcacatt gtcttctgtt
     5761 tctaaacgat ctttgacttc attgagcact aaatcactga cgatcatatc tttatacgta
     5821 ttgatcattt gcaggttcgt attgacatca ttgacatttg cagtgtctga ttgtggcagt
     5881 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
     5941 cctgagatgg ccaagccagc aaacattgag atgatgatta ttaccaagtg tttgcgtaat
     6001 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     6061 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcactg
     6121 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgctta
     6181 atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc aaagttcatg
     6241 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     6301 agactcaata cttttttcgc tacgccttct acgatctttc tttggcgccc ttgtctaccg
     6361 taatcaccat ttgggtcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     6421 aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     6481 acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca
     6541 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
     6601 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     6661 gtatcacgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     6721 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc
     6781 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     6841 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     6901 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat
     6961 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     7021 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctcagca
     7081 acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat ctgctctatt
     7141 tgactgagca ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttt
     7201 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgaat atcagcgaat
     7261 cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca
     7321 cgtacacgag cgacatctgc acttgtcacc ttttctttga tgatgacatc gtctacagct
     7381 tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt
     7441 tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca
     7501 gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag
     7561 gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaaatt
     7621 ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt
     7681 tgattggtga tggcagattc tgcataattg atccgttgct gatgttgccg ctgattattg
     7741 atatggtatc caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat
     7801 gacttgacga atggcttttc catagattca ctcct
//
LOCUS       DASJRQ010000110         7712 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJRQ010000110 DASJRQ010000000
VERSION     DASJRQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.112"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..2344
                     /locus_tag="U4A80_002401"
     CDS             17..2344
                     /locus_tag="U4A80_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1976006.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2470..2799
                     /locus_tag="U4A80_002402"
     CDS             2470..2799
                     /locus_tag="U4A80_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1976007.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2839..3843
                     /locus_tag="U4A80_002403"
     CDS             2839..3843
                     /locus_tag="U4A80_002403"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1976008.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3840..4286
                     /locus_tag="U4A80_002404"
     CDS             3840..4286
                     /locus_tag="U4A80_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1976009.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="U4A80_002405"
     CDS             4436..5251
                     /locus_tag="U4A80_002405"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1976010.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="U4A80_002406"
     CDS             5457..6629
                     /locus_tag="U4A80_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1976011.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="U4A80_002407"
     CDS             complement(6839..7285)
                     /locus_tag="U4A80_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976012.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="U4A80_002408"
     CDS             complement(7282..7476)
                     /locus_tag="U4A80_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976013.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2597 a         1448 c         1532 g         2135 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggatttttt gctatagttc tgttattaag tatgcaataa taaaatatgt
     7681 acgttatttt attattagat tattgacatt ta
//
LOCUS       DASJRQ010000111         7218 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJRQ010000111 DASJRQ010000000
VERSION     DASJRQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.113"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="U4A80_002409"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="U4A80_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1976014.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /locus_tag="U4A80_002410"
     CDS             complement(920..1678)
                     /locus_tag="U4A80_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1976015.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="U4A80_002411"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="U4A80_002411"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1976016.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="U4A80_002412"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="U4A80_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1976017.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="U4A80_002413"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="U4A80_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1976018.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="U4A80_002414"
     CDS             complement(4434..5291)
                     /locus_tag="U4A80_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1976019.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="U4A80_002415"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="U4A80_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1976020.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2281 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttatttat
//
LOCUS       DASJRQ010000112         7183 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJRQ010000112 DASJRQ010000000
VERSION     DASJRQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.114"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..1062
                     /locus_tag="U4A80_002416"
     CDS             52..1062
                     /locus_tag="U4A80_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1976021.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1131..2075)
                     /locus_tag="U4A80_002417"
     CDS             complement(1131..2075)
                     /locus_tag="U4A80_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1976022.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2059..4779)
                     /locus_tag="U4A80_002418"
     CDS             complement(2059..4779)
                     /locus_tag="U4A80_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1976023.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4776..5996)
                     /locus_tag="U4A80_002419"
     CDS             complement(4776..5996)
                     /locus_tag="U4A80_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1976024.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6277..7122)
                     /locus_tag="U4A80_002420"
     CDS             complement(6277..7122)
                     /locus_tag="U4A80_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1976025.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         2122 a         1366 c         1147 g         2548 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg
     1081 aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct
     1141 tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt
     1201 tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga
     1261 tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca
     1321 tggtgtgcta aaaccatatg acgtaatacg agaacatctt cgtcagcttc gctgattttc
     1381 aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt
     1441 cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca
     1501 tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc atcggcgatt
     1561 gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga
     1621 tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag
     1681 cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct
     1741 tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca
     1801 tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct
     1861 cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa
     1921 gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat
     1981 ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac
     2041 tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac
     2101 gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat
     2161 ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc
     2221 ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga
     2281 gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata
     2341 gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt
     2401 ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg
     2461 taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag
     2521 tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata
     2581 aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg
     2641 attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg
     2701 cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc
     2761 cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac
     2821 aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat
     2881 ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg
     2941 atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat
     3001 tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg
     3061 gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac
     3121 gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt
     3181 atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg
     3241 tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag
     3301 atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt
     3361 tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa
     3421 aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc
     3481 catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa
     3541 tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt
     3601 agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc
     3661 cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca
     3721 atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa
     3781 tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag
     3841 ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg
     3901 cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga
     3961 gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt
     4021 gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa
     4081 ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg
     4141 ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc
     4201 ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt
     4261 gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc
     4321 cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc
     4381 catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc
     4441 gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt
     4501 tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag
     4561 ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc
     4621 ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc
     4681 ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca
     4741 ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa
     4801 aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt
     4861 tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa
     4921 tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt
     4981 ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt
     5041 atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc
     5101 aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca
     5161 tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct
     5221 ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt
     5281 tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta
     5341 gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc
     5401 acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg
     5461 aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg
     5521 taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga
     5581 cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc
     5641 tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct
     5701 ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac
     5761 cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa
     5821 ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt
     5881 gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc
     5941 agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa
     6001 aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg
     6061 aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt
     6121 tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt
     6181 cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt
     6241 atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa
     6301 tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat
     6361 ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc
     6421 atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa
     6481 acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc
     6541 cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa
     6601 ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctataat
     6661 ttcctcaata ttattataag ggattgaaca aatagatttt tcatactcaa gctcatagtt
     6721 tagctctttg atagaaaatt cactccctac ctcgtgaaaa atttttttca ttttattttt
     6781 ataattctga gattcataac ttccgagatt tgtatatatt ttggttggaa atttcgatag
     6841 gacagaatca atttttttta catcattttt atcgaaggaa gactcaaata tctttttttc
     6901 tccatcaaat ccaacagaac catttaaccc tactgctggt atccttaaac cagatttttt
     6961 gagaatatta ttaatgtctt ctacttctct tccagaacaa atgaatggaa aacagtcttc
     7021 cggtaaatgt ttcattgttc ttgcattttt ttgactaatg ctctggtcag aattcaaaag
     7081 agtaccgtct aaatctatac caattagttt catttcatcc atgtctccta tacgttgatt
     7141 ataaattcac tgcttattgt aagtctaaat ttctcacaaa aca
//
LOCUS       DASJRQ010000113         7169 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJRQ010000113 DASJRQ010000000
VERSION     DASJRQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.115"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..427
                     /locus_tag="U4A80_002421"
                     /pseudo
     CDS             92..427
                     /locus_tag="U4A80_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            592..819
                     /locus_tag="U4A80_002422"
                     /pseudo
     CDS             592..819
                     /locus_tag="U4A80_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            945..1916
                     /locus_tag="U4A80_002423"
     CDS             945..1916
                     /locus_tag="U4A80_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1976026.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2055..3368
                     /locus_tag="U4A80_002424"
     CDS             2055..3368
                     /locus_tag="U4A80_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1976027.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDSGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFVWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3411..4277
                     /locus_tag="U4A80_002425"
     CDS             3411..4277
                     /locus_tag="U4A80_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1976028.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4274..5122
                     /locus_tag="U4A80_002426"
     CDS             4274..5122
                     /locus_tag="U4A80_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1976029.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5136..6608
                     /locus_tag="U4A80_002427"
     CDS             5136..6608
                     /locus_tag="U4A80_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1976030.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6625..>7169
                     /locus_tag="U4A80_002428"
     CDS             6625..>7169
                     /locus_tag="U4A80_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1976031.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT         2222 a         1225 c         1552 g         2170 t
ORIGIN      
        1 gtctacttat aatttacttt ggaggttttg aatgaatgaa attaagcgca ataaaaattc
       61 ttttggtgtt tatttaggag agcctagctc ggaacagtta aataaaggtg aagctcgtca
      121 tgctctatgt cgaaatatat tttatggtag aaaaggcaaa ctttatcaaa cttattacga
      181 cggtatggag gaacagctca acgcattagg attagtaact aatgctatca tatattggaa
      241 ttcactatat ttagaaaaag tctttgaaca aattaaaacg gaagggttcg actgttccga
      301 agaagtgtta cgcaagcaat ctccattaat tagtgatcac attaattttg ttggcaaata
      361 tagtttccgc tatgaaaaag aattatcgaa tggaatacta cgtccgttaa atatagagaa
      421 ttattgagaa tgttcgtctt ttggttccta ccgtgcattt tcgcacggat gttccagata
      481 ctatttttga atgtataaaa ttgaaaacac aagaatacta ataaatcaac gattcttgtg
      541 tttttaattt ttcactaccg tgcattttcg tttctatgtt ccatgtaccc caatcttgaa
      601 aaattaatta aagacttaga taaagccatt gacgacttag tcgtggtgct accggagtat
      661 caatgtttaa ccagtatccc aggtattggt cctgtttagg ctgctggaat actcgctgaa
      721 attggtccaa ttgaacgttt ttctgatgaa ataaaattga caaagtatgc cggcctatat
      781 tggcgacaga gtcaatccgg taaatctgaa tacgaaaatt tttttacgaa aagcttcacg
      841 tctaatatct attttaaaat aaaaatattc ctgctaattt ggaatcgttt ccttcttttg
      901 gtataataat acctgaaatt cttttgagag gagttgttcg tgaagtgaaa actactatga
      961 aggatgttgc tgcgttagca ggagttggtg tgggtacggt atctagagta ataaatggag
     1021 taaaggtgaa agaggttacc aaagaaaaag taatggctgc aattagtgag cttaattatg
     1081 agcctgatga atatgccaga ggtatgaaaa cgaatagtag caatacaata gctcttattt
     1141 tacctacgat ttggcatccc ttcttttccg agtttgccta ctatgtagag gaagctctca
     1201 gccaaaaaaa ttataagctt tttttgtgta atgcgaacgg cagtgcggaa aaagaaaaag
     1261 aatatatcca aatggtcaag cagagtaaag tagacggaat tataggtatt acctattcag
     1321 acattgataa atatgtatca tcaaatttac catttgtcag tatagaccgc catttttcgg
     1381 aacaggtttc ttatgtaacg gcagataatt atttaggtgg gcaaattgcc gctcaagaac
     1441 tacttactcg taactgtaaa aacttgtgct atgttggggg tgtttcacct taccctaacg
     1501 aaacaaacaa tcgtaaagct ggtttttaca atatttgtga ggaaaataaa atagctgtga
     1561 aggtattgga tatgcctgaa ccaattagca atttagagga acaattgctc gctttttttt
     1621 cggataatcc tgaaattgat agcattttta ccatcaatga tttcatggct ttggatgtga
     1681 taaaagccca aaaaaaacta gggaaggaag cacctaagga ttttcaaatt attggttttg
     1741 atggagtcaa gctatcgttg caaaaagact atttactatc aacgattgta cagccgatag
     1801 aggaaatggc caaggtttca gtagaaacac tcatggcgat gattttaaat gaagaaccgc
     1861 ctaagcgtgt ggtagtaccg gttttctttc acgctggaga gacgactaaa gagtaaaaaa
     1921 ccggtttata acgatttttt tgagataggt tgacagaaag tgatttcaat gatatcttaa
     1981 aaccaattaa ttggaaacgt taccaattaa ttgaaaaata aaacggaaac gtttcaaaaa
     2041 taggaggaac atcgatgaaa aagagaatgt tagcaattgc agcaatttta gcattaacag
     2101 ctggaggact tgcaggatgt ggcagtaagg agaaaagttc aggtagcagt gattcaggag
     2161 aaattaagat ttgggttcag ttttctgatg aaacgcctga aggaaaagct tgggcagaga
     2221 ttgtgaacaa ttttaatgat gaatacgagg gtgaatataa agctgttact gagtatattc
     2281 ctcgtagcgg aagcggcggt ggatatgagg ataaagtaaa tgctgcaatt acaaccaata
     2341 cccttcctga tgttattacg ttagatggac cgaatactgc agcttatgct cattcaggag
     2401 tcattgctcc attagatgat tatttgaaag atgcggacat ggatgatgta ttagacagca
     2461 ttattcagca aggaacctac gatggcaaaa tgtatgcgtt tggtttctca gagtccagtg
     2521 tgggtgtcta ttacaataag tcgttgttca aagatgctgg aattacagaa gatcaattgc
     2581 cgacgttaga agatccttgg acttggacgg aatttcaagc aatttgccaa aagctgactg
     2641 ataaatacaa aacgcctgca attgatatgc aaatggcgtc gggagacgaa atgctgacct
     2701 acgcatatac accatttgta tggtcgaatg gcggcgatgt cgttaatgct gatggaacca
     2761 aagccgaaga ctattttaac ggtaaaaaat ccgttgaagc gttggaattt attcaagaat
     2821 tggttgaaaa aggttacacc acaatcagtc ctattgaaaa aggatttgaa actggcaaat
     2881 atgccatgct gctgagcggt tcttggacaa tggcagattt agaggaaaac tatcaagaga
     2941 ttgattatgg tattttgcct taccccgttt cagatgcaac tggggagtta gtttcaccaa
     3001 ccggaagctg gcagttagca atgactgaga aaacagagaa aaaggatgct tctgcggcct
     3061 ttattaagtt tgcaaccagt acagaatcta gtcgtattat gagtttaggg aacagtgttc
     3121 tacctatcag aaattcaaca gttgatttaa ttaaagatga agtgtcggaa ggaatgcagg
     3181 tattaattaa gcaaaaccaa atttctggac atgcccgccc agtggttgta gcttatccgc
     3241 aagtatctag agcattccaa caagcagttc aagatattag ctacttcgag gaaaacccag
     3301 atgtacaaaa agtagcagat agtcgcgctg ctgaaatgca acaggcaatt gatacttcac
     3361 taaaatagaa gtttctaaag ggggccaatc aagtgttaaa aaaaccgaca atgaaagaca
     3421 atttgatagg ttacggcttt ctagcgccag ctttgctgct gctgacgatt tttctgatca
     3481 tacccgtaat catggtaatt tattatgcct ttactgatta ttatttgtta acgccagatg
     3541 ctagacaatt tatcggctta gaaaatttta agcgactgtt cgctgatcca atttttgtaa
     3601 aaagtatttg gaacacggct aaatttgttg tttggattat tccggtccaa ttaggggcag
     3661 ctttaggaat ggcacttatt atcaacaaaa atcgtaaagg aaatatcttt tttaaagtag
     3721 ccttttttgc gccagtcgtg atgtccttag tcgttatttc gattttatgg ttgtacttgt
     3781 taaatcctaa tgaaggtctg gttaatacgc tatttgcaaa gattggaatt gcttcgcagc
     3841 cttttttgac tagtcccaaa caagcaatgt atacgcttgt tttcgtatcg gcatggcaag
     3901 gtgcaggata tcagatgctg ctgtttttag ggggaatgca aaatattccg caagacgttt
     3961 atgaggcagc cgaaattgat ggtttttcta agtttcagca atttcgctat atcaccatgc
     4021 cattattaaa gcccacggcg atttttattt tacttaccac attgatttcg gcctttaaac
     4081 taatcattca gccaatggta atgactcaag gtggaccaat gaattctact atgacaatgg
     4141 tttattacat ttaccaaact ggatttacag accgtatggt aggctactcc agctccattg
     4201 cgttaatctt taccactatg attggtttaa ttacaattgc tcaaagaaaa ttggtaaagg
     4261 aggatgatcc agcatgacca agaaatttgg accgctaatg atcgtagaat attgtttgct
     4321 gttcctttta gcggcattat tcattttccc aatgctgtgg atggtagttt cctctatgaa
     4381 gcctgaagca gatgtctatc aaaatttagc ttccttcaaa gcttttttac ctagtttgaa
     4441 tccagctaat tggtttaaaa cttatcaaga agttatttcc cggttcagtg tgggaactta
     4501 tttattaaac agtatttttt atggtttaac ctttgcctgt gggtcaatca ttgtaaactc
     4561 tttggccggt tttgcctttg cgaaggttaa tttttcaggc aagaaaatta tctttggagc
     4621 tttgttagct ctgctgattg tcccgatgga gacggtttta attccgcaat ttaccatcgt
     4681 taattcacta ggcttagtga atactcgctt ggcagtgatt ttaccagcga tggccagcgc
     4741 gtttaatatc tatttgttta gaaatttctt tatcgcgatt ccagaagaaa tcattgaatc
     4801 agcgaaatta gatggtgcaa atattgttaa aattttcttt cacatcatgc tgccaatgtc
     4861 taaaccagcc atagccacag taggtgtctt atcctttatc tccagctgga atgattatat
     4921 ttggccgtta atggtcttaa ctgacagcag caaattttca atgcaagtgg caattactac
     4981 gatcaatact acacagccag tttatatcaa tcaagtgatg gctgttttaa ccattgcaac
     5041 gattccactg attattgttt atatcgttgc acaaaaatat attctgcagg gacttggcgg
     5101 ttctggaacg ggtataaaat aggagtgaat agtgaatgtt gcaaaaactt ttaaaggcca
     5161 atgagtttat tgagaaaaat gctcaaacag tcaatcaaga ataccggaat aaatttcatt
     5221 tgatggctcc gattggctgg atgaatgatc caaatggttt tgtttattat caagaggagt
     5281 atcacttgtt ttatcaatat tatccttacg acagcatctg gggaccaatg cattggggcc
     5341 atgctaaaag caaggatcta attcactggg agcatttgcc agtagcactg gcaccaggtg
     5401 aaagctatga caaggaaggc tgtttttccg gcagtgccat agaaaaggat ggcaagctgt
     5461 atttgattta tacaggacac aatgttgtgg aagggcaagt tcgacaagtt cagtgtttgg
     5521 cagtttcaga agatggcatt cactttgaaa aatatgccgg aaatccaatc atcgcagaag
     5581 atcacttagc ttctgttgca acgaccgaag atttccgcga tcccaaagtg tttcagcgaa
     5641 aagaagacta ttttactgta gtcgctacta aaactgcaga tgctcgtgga caaattgtga
     5701 tgttccattc caaagattta ctaacttggg agttttattc cgtattgctg gagggaactc
     5761 cagaacaagg cattgtatgg gaatgcccag acttgtttga actagatggc aaagaagtct
     5821 tgattctttc tcctattcaa atgactccac aaggcaatgc ttaccaaaat acgaactcaa
     5881 ctgttgcttt catcggtact gtcgattggc aaacaggcaa atttctagta gagaattttc
     5941 atgaaatcga tggcggttta gatttttatg caccccagac ctgtgaaaac gaagcaggcc
     6001 aaagaataat gattgcttgg atgcaaatgt ggcagcgtaa aatacctacg aatgatctgg
     6061 gccatggttg gtcaggttcc atgaccttgc ctagagagct gagggtggct gataatcaat
     6121 tgttgcaacg gccagtttca tcaatttatc aagtaattga tccgcaggaa aggattgaat
     6181 gggcgaagac tgaagagaat cgcttcttac tgaaggactt agtatatgat cagcactatt
     6241 tacgcttgga aatcaattta aaacaggcaa cgaaactaga acttcactat gcgaagggac
     6301 ccgaccatac attactgctt aactatgatg ttgaacggga gtgctttact gcttccaggg
     6361 aacacatgcc gtatgattta tcaggagctg aaaaggaagt gttgaaaaag cgcagtgtga
     6421 cgattccttt ggaaaataat tgtctaacca tcgaaatttt ccgtgatact tcagctattg
     6481 agctttttct aaatgatcgt ctgactatga gtacaacttt ttatgagaca gaaaaaggac
     6541 aggattttct tttgctggca gacagtgaag taaccttttc attggaaagc ggcttaatta
     6601 atctatagaa gggagagctc agtgatgatt tatggttcaa tcgaagcagg cggcacaaaa
     6661 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     6721 acgacacctg aggaaacaat acctttagtc attgacttct tcaaacaata ccaagcagat
     6781 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     6841 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     6901 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     6961 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     7021 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     7081 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     7141 tttcatggca actgtttaga agggatggc
//
LOCUS       DASJRQ010000114         6779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJRQ010000114 DASJRQ010000000
VERSION     DASJRQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.116"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..1613)
                     /locus_tag="U4A80_002429"
     CDS             complement(60..1613)
                     /locus_tag="U4A80_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1976032.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1761..2930
                     /locus_tag="U4A80_002430"
     CDS             1761..2930
                     /locus_tag="U4A80_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1976033.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3056..4135)
                     /locus_tag="U4A80_002431"
     CDS             complement(3056..4135)
                     /locus_tag="U4A80_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1976034.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4244..4684)
                     /locus_tag="U4A80_002432"
     CDS             complement(4244..4684)
                     /locus_tag="U4A80_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1976035.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4741..5151)
                     /locus_tag="U4A80_002433"
     CDS             complement(4741..5151)
                     /locus_tag="U4A80_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1976036.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5332..5766)
                     /locus_tag="U4A80_002434"
     CDS             complement(5332..5766)
                     /locus_tag="U4A80_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1976037.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5866..6714)
                     /locus_tag="U4A80_002435"
     CDS             complement(5866..6714)
                     /locus_tag="U4A80_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1976038.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2406 a         1238 c         1125 g         2010 t
ORIGIN      
        1 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
       61 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
      121 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
      181 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
      241 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
      301 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
      361 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
      421 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
      481 tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg ttcttgataa
      541 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
      601 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
      661 aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
      721 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
      781 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
      841 aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt gaattctttt
      901 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      961 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     1021 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     1081 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     1141 tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa tgcattaatc
     1201 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact taaactgata
     1261 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     1321 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     1381 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     1441 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
     1501 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
     1561 gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg catgaaagag
     1621 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
     1681 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
     1741 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1801 taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa acagccaaat
     1861 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1921 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1981 actattcagg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     2041 ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     2101 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     2161 tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag cggcagacaa
     2221 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
     2281 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
     2341 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
     2401 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
     2461 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2521 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
     2581 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     2641 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     2701 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     2761 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
     2821 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     2881 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     2941 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
     3001 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     3061 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     3121 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     3181 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     3241 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     3301 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     3361 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     3421 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
     3481 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     3541 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     3601 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     3661 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     3721 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     3781 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
     3841 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     3901 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     3961 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     4021 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     4081 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     4141 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     4201 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     4261 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     4321 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     4381 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     4441 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     4501 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     4561 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
     4621 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     4681 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     4741 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     4801 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     4861 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     4921 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     4981 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     5041 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
     5101 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
     5161 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     5221 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     5281 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
     5341 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
     5401 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     5461 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     5521 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     5581 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
     5641 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     5701 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
     5761 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     5821 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     5881 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     5941 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     6001 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     6061 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     6121 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     6181 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
     6241 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     6301 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     6361 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     6421 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
     6481 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
     6541 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
     6601 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
     6661 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
     6721 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagt
//
LOCUS       DASJRQ010000115         6732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJRQ010000115 DASJRQ010000000
VERSION     DASJRQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.117"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..909
                     /locus_tag="U4A80_002436"
     CDS             19..909
                     /locus_tag="U4A80_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1976039.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(982..1806)
                     /gene="nadE"
                     /locus_tag="U4A80_002437"
     CDS             complement(982..1806)
                     /gene="nadE"
                     /locus_tag="U4A80_002437"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1976040.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1818..3290)
                     /locus_tag="U4A80_002438"
     CDS             complement(1818..3290)
                     /locus_tag="U4A80_002438"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1976041.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3927..4289)
                     /locus_tag="U4A80_002439"
     CDS             complement(3927..4289)
                     /locus_tag="U4A80_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1976042.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4512..5264
                     /locus_tag="U4A80_002440"
     CDS             4512..5264
                     /locus_tag="U4A80_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1976043.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5404..6327)
                     /locus_tag="U4A80_002441"
     CDS             complement(5404..6327)
                     /locus_tag="U4A80_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1976044.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6342..6614)
                     /locus_tag="U4A80_002442"
     CDS             complement(6342..6614)
                     /locus_tag="U4A80_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976045.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2050 a         1347 c         1154 g         2181 t
ORIGIN      
        1 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
       61 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
      121 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
      181 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
      241 cttcgtgtcg ataaaatcga tattagtttt ccagaaaaaa tattcaatgc ttttgatacg
      301 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
      361 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
      421 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
      481 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
      541 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
      601 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
      661 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
      721 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
      781 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
      841 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
      901 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
      961 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
     1021 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
     1081 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
     1141 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
     1201 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
     1261 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
     1321 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
     1381 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
     1441 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
     1501 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
     1561 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
     1621 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
     1681 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
     1741 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
     1801 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
     1861 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
     1921 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
     1981 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
     2041 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
     2101 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
     2161 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
     2221 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
     2281 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
     2341 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
     2401 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
     2461 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
     2521 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
     2581 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
     2641 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
     2701 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
     2761 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
     2821 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
     2881 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
     2941 acttggatca atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
     3001 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
     3061 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
     3121 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
     3181 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
     3241 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
     3301 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
     3361 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
     3421 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
     3481 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
     3541 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
     3601 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
     3661 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
     3721 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
     3781 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
     3841 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
     3901 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
     3961 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
     4021 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
     4081 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
     4141 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
     4201 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
     4261 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
     4321 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
     4381 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcatgagaa
     4441 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
     4501 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
     4561 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
     4621 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
     4681 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
     4741 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
     4801 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
     4861 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
     4921 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
     4981 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
     5041 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
     5101 ataagaagtg gttatcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
     5161 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
     5221 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
     5281 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
     5341 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
     5401 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
     5461 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
     5521 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
     5581 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
     5641 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
     5701 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
     5761 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
     5821 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
     5881 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
     5941 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
     6001 ttcaagatac cttcgctctt tttctttctg atagcccgtc ggtaaaagtg tcactttgta
     6061 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
     6121 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
     6181 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
     6241 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
     6301 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
     6361 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
     6421 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
     6481 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
     6541 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
     6601 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
     6661 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
     6721 agtctcttat tt
//
LOCUS       DASJRQ010000116         6331 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJRQ010000116 DASJRQ010000000
VERSION     DASJRQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.118"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4A80_002443"
     CDS             complement(99..1229)
                     /locus_tag="U4A80_002443"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1976046.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U4A80_002444"
     CDS             complement(1878..3080)
                     /locus_tag="U4A80_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976047.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U4A80_002445"
     CDS             complement(3140..3343)
                     /locus_tag="U4A80_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1976048.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U4A80_002446"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U4A80_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U4A80_002447"
     CDS             4880..5692
                     /locus_tag="U4A80_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1976049.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1886 a         1300 c          998 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag a
//
LOCUS       DASJRQ010000117         6293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJRQ010000117 DASJRQ010000000
VERSION     DASJRQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.119"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(185..511)
                     /locus_tag="U4A80_002448"
     CDS             complement(185..511)
                     /locus_tag="U4A80_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976050.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(557..919)
                     /locus_tag="U4A80_002449"
     CDS             complement(557..919)
                     /locus_tag="U4A80_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEN1976051.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(956..1170)
                     /locus_tag="U4A80_002450"
                     /pseudo
     CDS             complement(956..1170)
                     /locus_tag="U4A80_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1298..1633
                     /locus_tag="U4A80_002451"
     CDS             1298..1633
                     /locus_tag="U4A80_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976052.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1709..3706)
                     /locus_tag="U4A80_002452"
     CDS             complement(1709..3706)
                     /locus_tag="U4A80_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976053.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3804..4079)
                     /locus_tag="U4A80_002453"
     CDS             complement(3804..4079)
                     /locus_tag="U4A80_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1976054.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4311..5045
                     /locus_tag="U4A80_002454"
     CDS             4311..5045
                     /locus_tag="U4A80_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976055.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5084..6226
                     /locus_tag="U4A80_002455"
     CDS             5084..6226
                     /locus_tag="U4A80_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976056.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2140 a         1149 c          900 g         2104 t
ORIGIN      
        1 attttttctt tttcatttct tttatattgt tataacagtg aataacataa aaattcccga
       61 caatcattgt aatatttttt tgtcacctgt attagtattt aatttatagg aaactataaa
      121 aactccctag tgaatttaca cctttgggag ttcctgtttg gacggtatgc ataatacatt
      181 taaatcaatt taacattaac acctcagcat atggtatctc cacatatata cggtctttct
      241 ttggatagta ctcaatattt atgttgatag cacttccatt gtctaatata gctttagccc
      301 ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc atcacatgat cgatttaagt
      361 tgtactgttc ttgtaaaatc tgtgttacca atggtttcga tgacttctct aatccagatg
      421 tacctgtcat ttttgaaaaa atgataccta taatagccaa gacaacgata actataatga
      481 taaacttctt gttcatattt actttattca actttttatc tcctatctcc aaaataattt
      541 gctcttatcc ctcttatcag tacattgaca tccctaaaag tacaccaaag attgctccag
      601 ctacccctgc tatcatcaaa atcgtaccca tttgttcatc tttatctcga tataagtagc
      661 ccataataac agcaacagca ccaaatacaa caggaataaa tgctaacgct actaccatag
      721 aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg atttgttcct
      781 tcatttcatt aattgtctgc tcagtacttt tctttactgc gtctgctcca gttgtctcat
      841 ctgtttcttg tagtgctccg caaaactggc aatacttact ttcatctgtt atttctttgc
      901 cacattttct acaatacatg tcttctcctc ctaaaaacat tcaattgttt attagttaac
      961 tgtttacttt attaaataca tcagaaaaca gctcattcat tggtacactt aatatctcag
     1021 aaagcatctt catttgatag ggtttaggaa tcgttctccc tgtttgccac gaacttacac
     1081 attgctgtgt aacattcatt tttttgctaa ctccgtttga gaaagtcctt tttcttttct
     1141 gtatcggctg agattactta cttttttcat gattctcccc tcctgtttta ttatatacaa
     1201 caatatgatg tatttcaact cataaaatat aaaaaaactt caaataccat tacaaataca
     1261 acaacttgtt gtactatact gagatggggg tgtattaatg aaacagaaac aatatgcaaa
     1321 ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa aaagaattag ctgaaaaaat
     1381 cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc ctaacctcga
     1441 tagcttaacc gctctttcta gagccctagg aacccctgta agccattttc tagacaatgt
     1501 tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca ctgaagatgc
     1561 agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac gcaatcagct
     1621 attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta ttgagatgaa
     1681 ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct tttctcttcc
     1741 ttactctccg tccatcatct cttgttacac ttgtaaattg ataaagagga actaattcaa
     1801 ctcctgataa tatcaattct tttattactt catctatccc tgctcctttt gctaggtaga
     1861 ctaaaaaact actacttgct ttcacattgt atagtctctt aatatcctta gaagctcgtt
     1921 caagagtgaa tccttgttta acaaagtagc ctctgttttc ttttaaagca ttttccacgc
     1981 tgttattccc aacattacgt agacgttcac aaacagtgtt agtaagttct ttctctgtca
     2041 ttagatatgt tccttgccac gataagccct gtaaaacttc atgtgcttca tctaaaatca
     2101 attgtttttc cttgctaagt tctgatcggg aattttgagg aaatactctt ttagcctctt
     2161 tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta aaatgaaatt
     2221 tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt gcattaaaaa
     2281 cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct ttctcttcct
     2341 ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct aattttttta
     2401 tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg tcataagttt
     2461 taccatagta aaaactcatt tttttagcta attgttcccc cgacaaaata caagtcacac
     2521 tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag acacgtcgca
     2581 tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat tcaagagtgt
     2641 caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac aagtctaggt
     2701 tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt agcatcacaa
     2761 ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa atggaatgcc
     2821 cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac agacatctta
     2881 agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt tcataaatct
     2941 cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt ttcatttctt
     3001 ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt tcatactctc
     3061 tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc ggaagctcag
     3121 taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta tatcctccaa
     3181 aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc tttttcaagt
     3241 attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat agaaagacaa
     3301 tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca aacgcagaat
     3361 tatgaactaa attgatagat aaaacctttt ctatgcttaa gtatctacta tcatctttac
     3421 tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc agaataatta
     3481 ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg cttggaataa
     3541 atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct ctatagttat
     3601 ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc tctaataatt
     3661 tttctttatc tacataaatt ggaatattaa tcattgatgt ttccatttgt gtttcctcct
     3721 tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct ttattactat
     3781 atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact atcaatatca
     3841 gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta ttgagaaaca
     3901 tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg atagtccata
     3961 tactctagag tgactccaga aataatgtct tctagcatag gctttagcac tgtaattatc
     4021 tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg tagagacata
     4081 tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt cgtatttttt
     4141 gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg attaccagtt
     4201 catcgacata cacataatac ctcgataaaa aagaggtgtc aatatctttg taaaatttat
     4261 ttacaacacc cctttttcga ggtatactaa ttttaggagg tgtggtcaat atgataaaac
     4321 gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata aaagctataa
     4381 gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac ggtcaattaa
     4441 ttgacaaaaa aactatctac gaatgggaga agggaaccta tttaccaata ccagagcgat
     4501 tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt ggcaatgtag
     4561 aagaatatat tttgggaatt attctttata gagatagcat tgtactagat ggtataacat
     4621 ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta cactctaatt
     4681 tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa tttattgcta
     4741 acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc ttaaaaatag
     4801 aagaattatt catcaatgct attgtagaag aatttgacaa taacattctt ttcctcacta
     4861 gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt caattgaaag
     4921 acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac aagctagaac
     4981 aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga ggtgatacca
     5041 tctagtggta agtactcctt gtaaactaag aggagatgat ttcatggcaa aaatatcaaa
     5101 gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg ccaccgatcc
     5161 acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac aagaagctca
     5221 aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga aagcacctga
     5281 tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca gtgttaaagc
     5341 ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag catttggtaa
     5401 gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg aatggcacag
     5461 taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac gctacggggt
     5521 tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac gaaaaaaaga
     5581 gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac tggatgcttt
     5641 taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat acacaggtat
     5701 gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta ataaagaact
     5761 acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc aatctccgaa
     5821 aacaagagct tctagacgag ttatctcttt agatactgag actctttcta tcctcaacaa
     5881 ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta gctctaaaga
     5941 gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg ttaatgattg
     6001 gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg ggtttagaca
     6061 tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc aacaacgatt
     6121 agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa ataatgcacc
     6181 taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga gctggtcaaa
     6241 atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat ata
//
LOCUS       DASJRQ010000118         6291 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJRQ010000118 DASJRQ010000000
VERSION     DASJRQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.120"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..230)
                     /locus_tag="U4A80_002456"
     CDS             complement(42..230)
                     /locus_tag="U4A80_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1976057.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="U4A80_002457"
     CDS             500..862
                     /locus_tag="U4A80_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1976058.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="U4A80_002458"
     CDS             914..2596
                     /locus_tag="U4A80_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1976059.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="U4A80_002459"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="U4A80_002459"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1976060.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="U4A80_002460"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="U4A80_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1976061.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="U4A80_002461"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="U4A80_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1976062.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2092 a         1094 c         1434 g         1671 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa g
//
LOCUS       DASJRQ010000119         6273 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJRQ010000119 DASJRQ010000000
VERSION     DASJRQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.121"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            46..1053
                     /locus_tag="U4A80_002462"
     CDS             46..1053
                     /locus_tag="U4A80_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1976063.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1065..2069
                     /gene="neuB"
                     /locus_tag="U4A80_002463"
     CDS             1065..2069
                     /gene="neuB"
                     /locus_tag="U4A80_002463"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN1976064.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2066..3214
                     /gene="neuC"
                     /locus_tag="U4A80_002464"
     CDS             2066..3214
                     /gene="neuC"
                     /locus_tag="U4A80_002464"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN1976065.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3187..3864
                     /locus_tag="U4A80_002465"
     CDS             3187..3864
                     /locus_tag="U4A80_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1976066.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3854..4996
                     /locus_tag="U4A80_002466"
     CDS             3854..4996
                     /locus_tag="U4A80_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN1976067.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5009..5986
                     /locus_tag="U4A80_002467"
     CDS             5009..5986
                     /locus_tag="U4A80_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1976068.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5986..>6273
                     /locus_tag="U4A80_002468"
     CDS             5986..>6273
                     /locus_tag="U4A80_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1976069.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSES"
BASE COUNT         2343 a          790 c         1206 g         1934 t
ORIGIN      
        1 aagaatcgaa ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa
       61 acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta
      121 aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg
      181 caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct
      241 caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa
      301 cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact
      361 caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca
      421 gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta
      481 ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt
      541 ggaaatgtca tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac
      601 aataaagata ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat
      661 gctgcaagat tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta
      721 aaaatgccgg caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat
      781 acacatgatg aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa
      841 ttatatgaag agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg
      901 tatatcattc caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt
      961 aaaatcactg catactcttc agatgcaaca gaattaatga gtggacagga atttattaga
     1021 atcgcaaaag agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat
     1081 atgtaattgc agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta
     1141 ttgataaagc taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata
     1201 agctagtttc caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca
     1261 gttctcaatt tgaaatgcta aaaaaattag aattatctca atctgatttt attagaataa
     1321 aagattattg tatacaaaat aaaattgatt ttttgtctac tccttttgat gaagaaagtg
     1381 cagactttct aaaacaaatt ggtgtacaag gatttaaaat tggctcagga gatttaacaa
     1441 atttaccttt tcttagaaaa atagatatgt atgggttgcc agttttatta tctacaggta
     1501 tggctgatat tcatgaggtc agtgaggctg tagattgttt catagatagt ccagttacta
     1561 ttttacattg tacttcaaat tatccggcta gttctgaaga tattaattta ttggcgatta
     1621 acacattaaa agaaatgttc gggatacccg ttgggtactc cgatcattca ttgggatatg
     1681 atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg
     1741 ataaggaact acctggtcca gatcataaag cttctttaga tcctcaggaa tttgcagatt
     1801 ttgtaaagca tattagaaac actgaaatat ttttaggtga tggtgttaag cgagcaatgc
     1861 cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct agtggtcact gagaatttaa
     1921 atgtgggaga tattcttaca gagaaatctc ttacaataaa acgtcctgga aatggaatag
     1981 ctcctaagtt tttatatcaa tatattggca aaaaagttaa aagaccaata agtgcagata
     2041 gtgttctgct tgaggaagat gttgaatgaa aaaaatactt atagtaagtg gaacacgagc
     2101 tgattatgga atatataaac ctgttgctga gaatttacta caagataatt gggatgtatc
     2161 atttgttgtt tctggaatgc atttatccca taaatatgga tttactaaag aattgatctt
     2221 agctgacgga tttagaatta gtggagaagt tcaaagtttg tttttagaga acaataaggg
     2281 aaacatggct cggtctgtgg cattagagat aaatggtttc acaaatatat ttgaacaaac
     2341 tagccctgat tttgtattat tactagggga tagaggagaa atgttggcag ctgcgatagc
     2401 ttgtttgtat cttggaatca aaacagcaca tattcacgga ggcgaagtgt ctggtactgt
     2461 agatgaatca ataagacatg ccataacgaa aatagcatct gttcattttg ctgctacgga
     2521 aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct tggagagttt ttcccgtcgg
     2581 agctcctaga atagatacta ttttaaataa tagattacct agttttagag atataaaaga
     2641 aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt
     2701 aaccgaacta gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca
     2761 atctcaacca attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa
     2821 aatgtaccag aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga
     2881 atacctgact atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat
     2941 tgaagctgca tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga
     3001 acaatctaaa aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa
     3061 taaaatacaa gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga
     3121 cggcaacagt tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga
     3181 tattaagtgg attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt
     3241 attggggctg gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta
     3301 atatatggat tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa
     3361 ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct
     3421 attggtgact gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa
     3481 tatggaatag ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga
     3541 actgttattt ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata
     3601 ataaacacag gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct
     3661 ccaggtgtat gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt
     3721 tctcaatgta tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact
     3781 attgttaagg atattccctc acattcctta gcttatggca atccagctaa aattaagaaa
     3841 gaaggaatta attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga
     3901 agaaaaatat gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt
     3961 ggataaattt gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt
     4021 aagttcaggt acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga
     4081 tattgtcttt gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg
     4141 ggctgtacca gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact
     4201 agagagcgca ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt
     4261 gaatttatat ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa
     4321 tgttccagtg atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc
     4381 aggcttattt gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc
     4441 aggtggagga gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc
     4501 aacacaagca agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg
     4561 tctaagcaat atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt
     4621 agctaaacga agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat
     4681 tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat
     4741 caatgattat aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa
     4801 cattgaaaca agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa
     4861 aatgtattac catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg
     4921 cttaccttct ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa
     4981 aaaaatattc aagtaggtgt gatattttat gaacatagga attattggtt taggttccat
     5041 ggggaaaaga agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt
     5101 tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa
     5161 tagtctaaca gaggcaaaaa atttgtttga aataaattca gtttttgttt gtacatcccc
     5221 tataacccat gaatttatta taaaagaggc tttaaattta gattgtaata tctttacgga
     5281 aatcaattta ttaaatgaat attatgatga aataatcgaa gtagctaaaa agaaagatag
     5341 attactttat ctatcttcta catttttgaa gagaaaagaa attcaatata ttaagtctaa
     5401 agtgacgcat aataaaaaaa tttcttatca ataccatgta ggacagtatt tacccgactg
     5461 gcatccttgg gaagattatc gcaatttttt tgtttcaaac aaaaaaacta atgggtgtag
     5521 agaaatattc gctattgaat taccttggat tatagataca tttgggaaag ttaaatcgtt
     5581 tactgtaatg acccagaaat tatccaatct tgatattgat tattcggaca gtttcactct
     5641 tattattgag catgaaactg gtatagttgg aactttaagt gtagatgttg tttcaagagt
     5701 agctaaaaga aatttaacta taattggtga aaagatttta atagaatggg aaggtaaccc
     5761 ggattctcta ttttcatgga atgaagaaac ttctagtatg acagatattc aattgtacga
     5821 tgaggttatg catgataata attattcaca tactattatt gaagatgcat atttagagga
     5881 gattaaagaa tttttcacgc ttttggaaaa gaaaatggaa ccccaatata cttttgaaaa
     5941 agataagtat attattaact taattaacga cattgaggta aattaatgat gaaagtaatt
     6001 ttttttgggt tagggtcaat tggaaagaga catctaaaaa atctaataag ctatggaaaa
     6061 aagaattcta ttaattttga tataacagct tatagtagca gtggaaaaaa agagtttgaa
     6121 gaaataacgt atattcaaaa tagagacgat ttggatacac attatgatat agcttttatt
     6181 acaaatccga cttttaagca tctggaaaca ctaaatgtaa ttgttgaaaa agttgattac
     6241 atatttttag aaaaacctgc tttttctgag agt
//
LOCUS       DASJRQ010000120         6028 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJRQ010000120 DASJRQ010000000
VERSION     DASJRQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.122"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(68..370)
                     /locus_tag="U4A80_002469"
     CDS             complement(68..370)
                     /locus_tag="U4A80_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976070.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U4A80_002470"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U4A80_002470"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="U4A80_002471"
     CDS             complement(1127..1900)
                     /locus_tag="U4A80_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1976071.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="U4A80_002472"
     CDS             2044..2394
                     /locus_tag="U4A80_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1976072.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="U4A80_002473"
     CDS             2375..3091
                     /locus_tag="U4A80_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1976073.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="U4A80_002474"
     CDS             3091..4539
                     /locus_tag="U4A80_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1976074.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="U4A80_002475"
     CDS             complement(4610..5119)
                     /locus_tag="U4A80_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976075.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2052 a         1050 c         1169 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtata tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaag
     5161 tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa
     5221 atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct
     5281 gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca
     5341 gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact
     5401 ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct
     5461 gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca
     5521 attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata
     5581 gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa
     5641 gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta
     5701 aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg
     5761 aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc
     5821 ggattatttt catacttttc acctctataa aaagtataga aaatgaatat gaataaagta
     5881 tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag
     5941 gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt
     6001 cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DASJRQ010000121         5954 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJRQ010000121 DASJRQ010000000
VERSION     DASJRQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.123"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..813
                     /locus_tag="U4A80_002476"
     CDS             <1..813
                     /locus_tag="U4A80_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1976076.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="U4A80_002477"
     CDS             807..1229
                     /locus_tag="U4A80_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976077.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="U4A80_002478"
     CDS             2399..3367
                     /locus_tag="U4A80_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1976078.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="U4A80_002479"
     CDS             3384..3908
                     /locus_tag="U4A80_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1976079.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="U4A80_002480"
     CDS             4284..4565
                     /locus_tag="U4A80_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976080.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="U4A80_002481"
     CDS             4567..4983
                     /locus_tag="U4A80_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976081.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="U4A80_002482"
     CDS             5613..>5954
                     /locus_tag="U4A80_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1976082.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DASJRQ010000122         5902 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJRQ010000122 DASJRQ010000000
VERSION     DASJRQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.124"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..1106)
                     /locus_tag="U4A80_002483"
     CDS             complement(324..1106)
                     /locus_tag="U4A80_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976083.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="U4A80_002484"
     CDS             complement(1171..2007)
                     /locus_tag="U4A80_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1976084.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="U4A80_002485"
     CDS             complement(2025..3434)
                     /locus_tag="U4A80_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1976085.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="U4A80_002486"
     CDS             complement(3448..5271)
                     /locus_tag="U4A80_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1976086.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1818 a         1276 c          957 g         1851 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat ca
//
LOCUS       DASJRQ010000123         5769 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJRQ010000123 DASJRQ010000000
VERSION     DASJRQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.127"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1014..1304)
                     /locus_tag="U4A80_002487"
     CDS             complement(1014..1304)
                     /locus_tag="U4A80_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976087.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="U4A80_002488"
     CDS             1706..1942
                     /locus_tag="U4A80_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976088.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="U4A80_002489"
     CDS             1949..3901
                     /locus_tag="U4A80_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1976089.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U4A80_002490"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U4A80_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1976090.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="U4A80_002491"
     CDS             complement(4797..5489)
                     /locus_tag="U4A80_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1976091.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DASJRQ010000124         5595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJRQ010000124 DASJRQ010000000
VERSION     DASJRQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.128"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(158..616)
                     /locus_tag="U4A80_002492"
     CDS             complement(158..616)
                     /locus_tag="U4A80_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1976092.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1960)
                     /locus_tag="U4A80_002493"
     CDS             complement(629..1960)
                     /locus_tag="U4A80_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN1976093.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3413)
                     /locus_tag="U4A80_002494"
     CDS             complement(1971..3413)
                     /locus_tag="U4A80_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1976094.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="U4A80_002495"
     CDS             complement(3400..4164)
                     /locus_tag="U4A80_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1976095.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="U4A80_002496"
     CDS             complement(4268..5143)
                     /locus_tag="U4A80_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1976096.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="U4A80_002497"
     tRNA            complement(5398..5482)
                     /locus_tag="U4A80_002497"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="U4A80_002498"
     tRNA            complement(5500..5571)
                     /locus_tag="U4A80_002498"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
BASE COUNT         1723 a         1175 c          831 g         1866 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaagcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa
     5581 ggcgaatgat gggaa
//
LOCUS       DASJRQ010000125         5458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJRQ010000125 DASJRQ010000000
VERSION     DASJRQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.129"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            148..1080
                     /locus_tag="U4A80_002499"
     CDS             148..1080
                     /locus_tag="U4A80_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976097.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1301..3064
                     /locus_tag="U4A80_002500"
     CDS             1301..3064
                     /locus_tag="U4A80_002500"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1976098.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3148..3606
                     /locus_tag="U4A80_002501"
     CDS             3148..3606
                     /locus_tag="U4A80_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1976099.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3715..4398
                     /locus_tag="U4A80_002502"
     CDS             3715..4398
                     /locus_tag="U4A80_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1976100.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4545..5159
                     /locus_tag="U4A80_002503"
     CDS             4545..5159
                     /locus_tag="U4A80_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1976101.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5175..5381
                     /locus_tag="U4A80_002504"
     CDS             5175..5381
                     /locus_tag="U4A80_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976102.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1958 a          856 c         1112 g         1532 t
ORIGIN      
        1 ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga atttgactcg
       61 cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga aaataaaaat
      121 ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca tttttttaaa
      181 agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat cgcaatcgga
      241 agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga aacgaagccg
      301 ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga cttacaagaa
      361 gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa tgagaacatg
      421 gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa tgacctatca
      481 ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc agaaaatttt
      541 attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg acaagtattt
      601 ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg aaaaattgta
      661 gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa tgaaaatttc
      721 attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg gagacaggaa
      781 atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg aacccaattt
      841 catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg ttcattagaa
      901 gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga tgctgctagc
      961 tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt aaaaggtgca
     1021 aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca tatttcttga
     1081 tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga aggtttttat
     1141 ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga aattttttta
     1201 aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt aacaaatcta
     1261 ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag tattacatgt
     1321 attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg aaatgaaaca
     1381 gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag gtgggcacgt
     1441 tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat ttgattcacc
     1501 aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc ttttaactgg
     1561 tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt ccaaccaaca
     1621 tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca gcttagcaac
     1681 aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag catttatcgg
     1741 tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg gtgttttaca
     1801 atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga atcatggcag
     1861 tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca ataacctatt
     1921 taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg aaacggtgat
     1981 ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg tccatacgga
     2041 aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt ggcatgtacc
     2101 atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat ttaatatgat
     2161 catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg ccgcagtcaa
     2221 tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc cagatcatta
     2281 ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt tggcttctca
     2341 gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat atgatcaatt
     2401 atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg gcggaacgat
     2461 ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat caagtatccc
     2521 gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc tagattgggc
     2581 gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg tagaacatgg
     2641 tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata atggaggaaa
     2701 agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag taaaagcttt
     2761 tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag aaaatgacgt
     2821 agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag aagatggtag
     2881 tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt caatgaacgt
     2941 attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc agctttatga
     3001 acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct tgaatattca
     3061 ataattcatt atgctcacca tccagataat tgtttatact agtatctgga tggttttttc
     3121 attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata tgtcaaaaag
     3181 ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc atttggggat
     3241 acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa gactggtaca
     3301 tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt tggtcaatta
     3361 gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat aaatgtgacc
     3421 atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga aggggatcgg
     3481 acattagctt attggcggaa agtccatgct gagtttttta aacatgctta taaagaagcg
     3541 ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga agtaattttc
     3601 ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga atagaaaagg
     3661 gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga tattgtggat
     3721 acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga aaacgagccg
     3781 ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg cggagcagtt
     3841 ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt tgatttaccg
     3901 gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac tgcgacagaa
     3961 aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga tgcaacttct
     4021 cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca cgaacacggt
     4081 cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca agaagatggt
     4141 tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa gacgcagaca
     4201 cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt aattatggaa
     4261 ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc tttttcagtc
     4321 gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt cgaagccgca
     4381 agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa agaaaaattg
     4441 atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc attttgtctt
     4501 caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt gaacggagtc
     4561 cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc atccctagac
     4621 cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca cccttcagtt
     4681 acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag agaaaacaag
     4741 atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt atccatatcc
     4801 ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct aatgaggaaa
     4861 gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca gtaccagcat
     4921 tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag atacggaata
     4981 aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat ttaaatgaaa
     5041 cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct gtaagccgtt
     5101 tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt cccgaataaa
     5161 aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca aaagaaatga
     5221 aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa gttccgccat
     5281 tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata gaaatgccag
     5341 atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt ccgaaaagtg
     5401 aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct ggtgcaac
//
LOCUS       DASJRQ010000126         5336 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJRQ010000126 DASJRQ010000000
VERSION     DASJRQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.130"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1019)
                     /locus_tag="U4A80_002505"
     CDS             complement(<1..1019)
                     /locus_tag="U4A80_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1976103.1"
                     /translation="MFLGKGEINIVKKIIKTLPLLWLLSSLILAICGFYGYTVLLRPE
                     NTIKQAQQITHPIQSEKEIKEIQQTTIGKATYNENQIQPVTPTEYAEAQLSYEDLVNQ
                     WGIGSLYIPSSGIYSKILAGMSNDNLMVGLGTYYPNQLLGKGNYVLMAHNLVQGGGVL
                     HNLPQSSVGSTIYATDFSKIYEYEITTNKIVNQSEGKLLDIPQEGDSPLMTIFRCEGG
                     LHTANRALIQARYVRSYSAENGSHDIKQALGLETTRNKTVNKQRLIDQQATSTKKTEA
                     AKESITPDKDTKKAKQTNKIEWCFTEKKAIYSNFQVFSILIFQLANAYPILIGLVFLV
                     GLSSC"
     gene            complement(1068..4232)
                     /locus_tag="U4A80_002506"
     CDS             complement(1068..4232)
                     /locus_tag="U4A80_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HEN1976104.1"
                     /translation="MKNKRNKWKVPKYLLMILGALAMFLGFGLSSQSVNADNITMKPG
                     EKGDIYYDASNVYTMHGTKHGQPWSDATAAMAVSPNNGATDRIVFCIQPGVPFITTNN
                     PDYEAVAVTDIPKDAQIASVIWNTFYAFGTNFTNEDRIVTQAVIWELLPQYGIKVDSI
                     SGIPDFQAKKQKLYDGIKEYKRLPEFNNKTIDLTFGETKKLTSSVDLNSFETQVSNSA
                     KVNWNIAADGKSVDVTPTDPNVTGGSISFKRSYMEGTPIALEKTGSQTVYLPSVKDPN
                     NYSVNFRIQTVGDITIKKVDKETGEVVPNTQFKVEFSGKGAPSAQTVTTDKNGLATIK
                     GINHGVHVKATEIAVPAPYVLGSAIGDSDVVEGDVVAGQTVTLTQRNQKAKGQILIEK
                     SGVETGKDMWNANYTLAGNVFEIHEGTETGAVVRTITTDEKGHAETGKDLPLGTYVIT
                     EKTASNGFANTFKPVTVKIEYANQTTAVIVKGAEGTNQEVVGSTLLTKEDAETGKETQ
                     GRATFDGAQYGLFHKDGTPVKWSENFKPTTSKGNKLEGDEVKFELSDEEQQASVEHLA
                     LGDYYWQELVAPEGYQIDNTKREFSITYKDQETKVIETQSTSQENVIKFSLDGFKYVD
                     SKSGDTKTGYNGIDFTLTPIDPTKGEQRKVTTETDENGYDGYWAFNEVPYGDYKMSEV
                     KAPEGYKTIKDLIINSKFDAEKREYTFTITEDGQKEPIKTLTVPEAKINEGSNVIHLS
                     KLFLTNKVKEMPTIRTKATVDGEKTFTPSKETPMQDVATITNVEKGVEYTNKIKLWRI
                     VNDDYDNAKVVWEGSHDFVAEGDTAEDVIKQMVDTSKDDNTVSYVFTEELFIKDGAKV
                     AEHNDLTNKEQTIRPVVPELPTIETLFVTKDGGKTFDPTKDQTLIDEVKATVPKEDIG
                     QTFYYVTQFHKIDKNGKVTVVGTDESEHKAGEMTYEFKAVYNYKANTLKDGEKLVATH
                     IAYTDKEHKNEYAKHFDLKNEKQTLTAKTPVTPATPTTPQKSIPQTSGSLINIGSLIV
                     LGIAAVAGAALYVTTKKKVNKNEK"
     gene            complement(4437..5330)
                     /locus_tag="U4A80_002507"
     CDS             complement(4437..5330)
                     /locus_tag="U4A80_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1976105.1"
                     /translation="MKLLKVLAVGVTVGGIALAINQEKVSAEEWTPRTVEQIKADIKG
                     NEYTIVWGDTLSGISQATNITVQKLADINKIANVDLIYAGNKLVFEGNVATVQNSAGE
                     TVAQTVIQDTDKVNPTQPVGQPAVSQDTTSNTTNSADQSVAPTTPAGDNGANTPEQPV
                     QPSTPSDNGNQGGTVTPNPDQNQGGNNNQDNQGNQGGDTGNNGNQDNQGNNNGNNNNG
                     NNNGGNDGGDVTPPQPVEKWTVWYSASDEANATYNIEKGTKVFDTEAEATAWIDQYAD
                     GLLAQGITSNSYGVSSFLVQE"
BASE COUNT         1396 a         1031 c          906 g         2003 t
ORIGIN      
        1 atacaacttg agagacccac aagaaaaact aacccaatca gaataggata agcattagct
       61 aattgaaata ttaggataga aaacacttgg aagttagaat aaatagcttt cttttctgtg
      121 aagcaccatt ctattttatt tgtttgcttt gcctttttag tatccttatc gggagtaata
      181 ctttcctttg ctgcttccgt tttcttagtt gaagtcgcct gttgatcaat tagacgttgt
      241 ttattaactg tcttatttct cgttgtttcc aatcctaaag cttgtttaat atcatgtgaa
      301 ccattttcag ctgagtaaga tcgaacatac ctagcttgaa ttaatgcacg attagctgtg
      361 tgtaatcctc cctcacatct aaaaatagtc attaaaggcg aatcgccttc ttgtgggata
      421 tccaataact taccctctga ttggttaaca attttatttg tagtgatctc atattcataa
      481 atcttgctaa aatcagtcgc ataaattgtt gatcctacgc tagattgtgg taaattatga
      541 agaactcccc caccttgaac taaattatgt gccatcaaaa cgtagtttcc ttttccaagt
      601 aactggtttg gataataggt tcctaagcca accattaaat tgtcattgct cattcctgca
      661 agaatcttgg agtatatacc agaagacgga atatagagag aaccaattcc ccactggtta
      721 acaagatcct cgtagctcag ctgtgcttcc gcatactctg taggcgtaac aggttggatt
      781 tgattttcat tgtatgttgc tttccctatt gtcgtttgtt gtatctcctt gatttctttc
      841 tctgactgta tagggtgagt gatttgctgt gcctgtttaa ttgtattttc gggacgaaga
      901 agaacggtat aaccataaaa tccacagatt gctaaaatca agctactaag tagccacaat
      961 aacggtaagg ttttaatgat tttctttact atattgatct ctcctttccc taaaaacaat
     1021 aactaaaata ggaaaagcac ctttttcaaa gtgcttcccc ttcattctta tttttcgttt
     1081 ttatttactt ttttcttagt tgtaacatac aatgctgcac ctgcaacagc agcaattcct
     1141 agaacgatta agcttccaat attaatcaat gatccagaag tttgtggaat tgatttttgt
     1201 ggtgttgttg gagttgctgg cgtaactggt gtttttgccg ttagtgtttg tttttcattt
     1261 ttcaaatcaa agtgtttggc atattcgttt ttgtgttctt tgtctgtata agcaatatga
     1321 gtcgcaacta atttttcgcc gtctttcaaa gtattcgctt tgtagttata aaccgctttg
     1381 aactcataag tcatttcgcc tgctttgtgt tctgattcgt ccgtacctac aacagtaact
     1441 tttccatttt tatcaatttt atggaattgt gttacatagt agaatgtttg accgatatct
     1501 tctttaggta cagttgcttt tacttcatca attaatgttt gatctttcgt tggatcaaat
     1561 gtttttccac catcttttgt tacgaataat gtttcaatag ttggcaattc aggcacgact
     1621 ggacgaatcg tttgttcttt attcgttaag tcgttgtgtt ctgcaacttt tgctccatct
     1681 ttaatgaata attcttccgt aaatacgtaa gagacagtat tgtcatcttt tgaagtatct
     1741 accatttgct taataacgtc ttctgctgta tcgccttcag ctacaaaatc atgagaacct
     1801 tcccaaacaa ctttagcatt gtcatagtcg tcgttaacga ttctccaaag tttgattttg
     1861 tttgtatatt cgacaccttt ttcaacgtta gtaattgtag caacgtcttg cattggtgtt
     1921 tcttttgaag gtgtaaatgt tttttctccg tcaacagtag cttttgtgcg gatagttggc
     1981 atttctttaa ctttattagt taagaataat ttgcttaaat gaatcacatt tgaaccttca
     2041 ttgattttag cttctggtac agtcaatgtt ttaattggtt ctttttgtcc gtcttcagta
     2101 atggtaaatg tgtattcacg tttttcagca tcaaatttgc tgttaatgat taaatcttta
     2161 attgttttgt agccttctgg agcttttact tctgacattt tgtagtcgcc ataagggact
     2221 tcattgaatg cccagtatcc atcatagccg ttttcatctg tttcagttgt tactttacgt
     2281 tgttcgcctt ttgttggatc aataggagtt aacgtgaagt caattccatt gtaaccagtc
     2341 ttagtatcgc ctgattttga atcaacatat ttaaagccat caagactaaa tttgattaca
     2401 ttttcttgtg aagtgctttg agtttcgatt acttttgtct cttgatcttt gtaagtaata
     2461 gaaaattcac gtttcgtatt atcaatttgg tatccttctg gagcaacaag ttcttgccag
     2521 tagtagtcac ctaatgctaa gtgttcaaca cttgcttgtt gttcttcgtc agataattca
     2581 aatttcactt cgtcgccttc taatttgttt cctttagaag ttgttggttt gaagttttca
     2641 gaccacttaa caggagtacc gtctttatgg aataaaccgt actgcgcccc atcaaaagta
     2701 gcacgtcctt gtgtttcttt gcctgtttca gcgtcttctt tcgttaacaa tgttgaacca
     2761 acaacttctt ggttcgttcc ttcagcgcct tttacaatta ctgctgttgt ttggtttgca
     2821 tattcgattt tcactgttac tggtttgaat gtgttagcaa acccattaga agctgttttt
     2881 tcagtaatta cataagtacc taatggcaaa tcttttcctg tttctgcatg acctttttcg
     2941 tccgttgtaa tagtgcgaac aactgcccct gtctcagttc cttcatgaat ttcaaataca
     3001 tttcctgcta atgtatagtt agcattccac atatcttttc ctgtttctac accagatttt
     3061 tcaatcagga tttgtccttt agctttttgg ttacgttgtg ttaaagtaac tgtttgtcct
     3121 gcgacaacat cgccttctac tacgtcagaa tcgccaatag ctgatcctaa aacataagga
     3181 gctggtacag caatttctgt tgctttgaca tgcacaccat gattaattcc tttgatcgtt
     3241 gctaaaccat ttttatcagt agtaacagtt tgtgctgaag gcgctccttt accactaaac
     3301 tctactttaa attgcgtatt aggaacaact tcgccagttt ctttatctac ttttttaatt
     3361 gtaatatctc caactgtttg aatacggaaa ttcacgctgt agttattagg gtcttttaca
     3421 cttggtaagt agactgtttg tgagcctgtt ttttctaatg cgattggtgt tccttccatg
     3481 tatgaacgtt taaaagaaat gcttccacct gtcacattag gatcagtagg agttacatcg
     3541 actgatttac catcagctgc aatgttccag ttaacttttg cactattaga aacttgagtt
     3601 tcaaaagaat ttaaatctac agaagaagtt aattttttag tttcgccaaa tgttagatca
     3661 atcgtcttgt tgttaaattc tggcaaacgt ttatattctt tgattccatc ataaagtttt
     3721 tgctttttag cctgaaaatc aggaatacca ctgatagagt caactttgat tccatattgt
     3781 ggtaataatt cccaaatgac agcttgtgtg acaattcgat cttcattggt aaagttagtt
     3841 ccaaaagcat aaaacgtatt ccaaatgaca gaagcaattt gtgcgtcttt aggaatgtca
     3901 gttacagcaa ctgcttcata atctggatta tttgtagtaa taaaagggac accgggctga
     3961 atacaaaata caattctatc agtagcccca ttattaggag aaactgccat tgcagctgtt
     4021 gcgtcgctcc atggttgccc atgcttcgta ccatgcatag tataaacatt acttgcatca
     4081 taatagatat cgcctttttc tcccggcttc atcgtaatat tatcggcatt tacactttgt
     4141 gaactaagtc caaagcctaa aaacattgcc aatgcaccta aaatcatcaa tagatacttg
     4201 ggaactttcc acttatttct cttgtttttc attttcacaa tattctccct ctcttcaatc
     4261 aatatataag tacagtaaaa aaagagcgcc acccaaaggc tcgctccacc gactgacatt
     4321 aaattttaga ttggaatcac ccccttaaag aaaataaaac ccttcgatat caatttgaaa
     4381 agggtaaaaa aaagacacct aagaattttc ttaagtgtct tggcaaacag attttattat
     4441 tcttgaacta agaatgaaga tacgccataa gaatttgaag taattccttg tgctaacaag
     4501 ccatctgcat attgatcaat ccatgctgtt gcttcagctt ctgtatcaaa tacttttgtt
     4561 cctttttcaa tattgtaagt agcgttagct tcatctgaag cagaatacca aactgtccat
     4621 ttttcaactg gttgtggtgg tgttacatcg ccaccgtcat taccgccatt gttgtttccg
     4681 ttgttgttat ttccattatt gtttccttga ttgtcttggt ttccattatt tccagtgtcg
     4741 ccgccttgat ttccttgatt atcttggttg ttatttccac cttgattttg atcaggattt
     4801 ggagttaccg ttccaccttg attcccattg tcagaaggtg ttgagggttg aactggttgt
     4861 tctggagtat ttgctccgtt atctccagct ggtgtagtag gtgcaacaga ttggtcagct
     4921 gagttagttg tattacttgt tgtatcttga gaaactgcag gttgccctac tggttgagtt
     4981 ggattaactt tgtctgtatc ttgaataact gtttgagcta ctgtttcgcc tgcgctattt
     5041 tgaacagttg caacattgcc ttcaaatact aatttgtttc ccgcatagat caagtcaaca
     5101 tttgcaatct tgttgatatc agccaatttt tgaacagtaa tgtttgttgc ttggctaata
     5161 ccacttaaag tgtcgcccca aacgatcgtg tactcattgc ctttgatatc agctttaatt
     5221 tgttctactg tacgtggtgt ccattcttct gcgctcactt tttcctgatt gattgctaac
     5281 gcaattcctc ctactgttac accgacagct aacactttca ataatttcat gtttta
//
LOCUS       DASJRQ010000127         5326 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJRQ010000127 DASJRQ010000000
VERSION     DASJRQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.131"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..546
                     /locus_tag="U4A80_002508"
     CDS             <1..546
                     /locus_tag="U4A80_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1976106.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="U4A80_002509"
     CDS             complement(716..1678)
                     /locus_tag="U4A80_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1976107.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="U4A80_002510"
     CDS             complement(1680..3119)
                     /locus_tag="U4A80_002510"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN1976108.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="U4A80_002511"
     CDS             3304..5250
                     /locus_tag="U4A80_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN1976109.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1716 a          912 c          889 g         1809 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa tgaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataa
//
LOCUS       DASJRQ010000128         5312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJRQ010000128 DASJRQ010000000
VERSION     DASJRQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.132"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="U4A80_002512"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="U4A80_002512"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1976110.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="U4A80_002513"
     CDS             complement(1298..2134)
                     /locus_tag="U4A80_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976111.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="U4A80_002514"
     CDS             complement(2318..3535)
                     /locus_tag="U4A80_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1976112.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3863)
                     /locus_tag="U4A80_002515"
     CDS             complement(3684..3863)
                     /locus_tag="U4A80_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976113.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3918..4001
                     /locus_tag="U4A80_002516"
     tRNA            3918..4001
                     /locus_tag="U4A80_002516"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..4819)
                     /locus_tag="U4A80_002517"
     CDS             complement(4241..4819)
                     /locus_tag="U4A80_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976114.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4825..5208)
                     /locus_tag="U4A80_002518"
                     /pseudo
     CDS             complement(4825..5208)
                     /locus_tag="U4A80_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DASJRQ010000129         5181 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJRQ010000129 DASJRQ010000000
VERSION     DASJRQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.133"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1413)
                     /locus_tag="U4A80_002519"
     CDS             complement(<1..1413)
                     /locus_tag="U4A80_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1976115.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(1424..2092)
                     /locus_tag="U4A80_002520"
     CDS             complement(1424..2092)
                     /locus_tag="U4A80_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1976116.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2222..2806)
                     /locus_tag="U4A80_002521"
     CDS             complement(2222..2806)
                     /locus_tag="U4A80_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1976117.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3137..3370)
                     /locus_tag="U4A80_002522"
     CDS             complement(3137..3370)
                     /locus_tag="U4A80_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1976118.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3385..4335)
                     /locus_tag="U4A80_002523"
     CDS             complement(3385..4335)
                     /locus_tag="U4A80_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1976119.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4332..5174)
                     /locus_tag="U4A80_002524"
     CDS             complement(4332..5174)
                     /locus_tag="U4A80_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1976120.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
BASE COUNT         1758 a          921 c          641 g         1861 t
ORIGIN      
        1 tttcgttatg tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat
       61 atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa
      121 ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc
      181 agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa
      241 attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa
      301 atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg
      361 ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa
      421 aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc
      481 gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt
      541 ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat
      601 gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt
      661 ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt
      721 gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag
      781 aacttgatca attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa
      841 tcctttattt tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa
      901 atttgaagac ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat
      961 agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga
     1021 aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc
     1081 aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct
     1141 aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag
     1201 cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag
     1261 tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt
     1321 aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt
     1381 atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta
     1441 agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat
     1501 agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt
     1561 cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca
     1621 gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta
     1681 tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt
     1741 tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag acactaacca
     1801 acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa
     1861 aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa
     1921 ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa
     1981 ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga
     2041 ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac
     2101 ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat
     2161 aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta
     2221 attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata
     2281 gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg
     2341 agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga
     2401 tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt
     2461 caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt
     2521 tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt
     2581 tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat
     2641 taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa
     2701 aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag
     2761 tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag
     2821 ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat
     2881 tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag
     2941 agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa
     3001 tagactcatc taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta
     3061 tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc
     3121 ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt
     3181 tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct
     3241 cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct
     3301 tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa
     3361 tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt
     3421 ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt
     3481 aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc
     3541 atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa
     3601 gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga
     3661 aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt
     3721 tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt
     3781 ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc
     3841 taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg
     3901 aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact
     3961 tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca
     4021 agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt
     4081 tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat
     4141 actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt
     4201 agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat
     4261 tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt
     4321 ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt
     4381 agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg
     4441 ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa
     4501 ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt
     4561 aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt
     4621 aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt
     4681 aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact
     4741 aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca
     4801 aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt
     4861 gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt
     4921 tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa
     4981 ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca
     5041 cccataagag acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct
     5101 ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata
     5161 aaattttgaa gcatattatt c
//
LOCUS       DASJRQ010000130         5119 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJRQ010000130 DASJRQ010000000
VERSION     DASJRQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.134"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..433
                     /locus_tag="U4A80_002525"
     CDS             62..433
                     /locus_tag="U4A80_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEN1976121.1"
                     /translation="MAIRVKVPKEIKDYQEKLMGGMSVRQLVFFVLAVLISVGVTAYC
                     VLVLHMSVDAAGYIVIPLLIPIVAFGWIRKEGMPLEKYGKIILNYYSKTGIRIYQTKE
                     RSNLNDFRKKRFQKVKETTKR"
     gene            381..2831
                     /locus_tag="U4A80_002526"
     CDS             381..2831
                     /locus_tag="U4A80_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1976122.1"
                     /translation="MILEKNDSKKSKKQPKDKQAKKVLKILAKQKKQRGKNQRRDAPP
                     LSSQRSLTYKAMYENGVCELKEGLYSRTFKFSDINYAIADQSEQELTFSSYCELLNSC
                     DSDTSLQISIINRSKDKKQFEKDLSYELVDDGLDVYRKEMNHMISNKIKYGNSSLQKE
                     KYITFNQRAKTLEQAIPALNRLQGDLEDQFSEMGCEVEELSGIERLRVVNDILRPNKK
                     LHFEYEDLLFSGLTTHSAIAPDSFDFKYRKDRFKMGDYYGQVLYLRNYPAELSDKLIT
                     NITDIPTNLQITLHIEPLDQQKGLDLVKTKLSFMEQQKIDEQKKAARSNYDPNLIPME
                     LRRSMDEAQELLDQMQGDNEKLFSFTFLVMAVDKDERKLDDTIEQILTAGRKNDCEFA
                     KLNYLQEEGLNSTLLWGNNFVPIHRTLTTASTAVFIPFTAQELMQPNGKYYGLNSVTK
                     NLVTLDRRKLKAPNGFILGTPGSGKSFATKKEMTAVLLSDPDAEVIVIDPEREYTPLA
                     EALNGEVIQISAGSKNHVNPMEINNDYADDDDPVILKTDFVLSLCEMLVGGNIGLSPT
                     QKTLIDRSCRFAYKKYFENGSGMPTLKDFSNILKDQPEDEARQLAVELELYTVGSLSV
                     FSHQTNVDTSKRFIVFDVKDLGKQLRSMGMLIILDQIWNRITSNRNAGRRTWVYIDEI
                     QLLFSNEYAENYFFELWSRARKWGAIPTGITQNVSTLLSSLNAERMLSNSDFVLMLNQ
                     SQRDRERLGELLHISSRQKKYITNAKEGAGLMYAGDSMIPLYDEFPKDTNLYKVMSTK
                     IEDVYKYQNEQREQGTTV"
     gene            2828..4675
                     /locus_tag="U4A80_002527"
     CDS             2828..4675
                     /locus_tag="U4A80_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1976123.1"
                     /translation="MNTDFRERIIRKQKTAKKFVVNRDNNQFKKKIVSTDYTKRAKKA
                     NHSGKIKSSRGTSYLIKKDSAKNVVKDVNSKAQKNGVEQGNATAELETSKTIGRGIKN
                     STKATKTVAKTTTKVTKKSKELIQTKASKKEVLTVVKKGSKKGLVNTSKAAKDTVINE
                     IQEFRGDSESQGINEAIQLKDFIDTGISGAKKTKKAANTAKKSSKALTKFGKSSYKYT
                     QRIAEIGIRRVQQVLSKEVAKALIAKLAIPLTLAVLILSIVTAVISTVNIFNFGAGDC
                     DTSANYDNITTKDIESNAKSIYSIIRKEIPESTPQGISGALGAMQFESQLNPSAVNPS
                     SGATGIAQWLGSRLTSLKEFAHKKGKKETDLGVQVEFLLSELNSSYYQSSKKILAMTD
                     VHEACKEWVMKFEGLSQDSSQWYLDQRNSYADHWYATLGTSDPIAEGTISNGADSELN
                     TLTCDLESDGGDIMEIAKSWLGWFHYDQIHPAPDLGTDLKNPNKNGRTDCSGFVWLVL
                     NKAGYKVPANMQWFTGSMASDARGSHQYLKQISETEAKAGDIVIVNQGAGAGSNGHTG
                     ILTEKWRGKDTKIIQEGGNGDSVNIEAFGTSFMSLLDGGDVVLARPIKK"
     gene            4695..>5119
                     /locus_tag="U4A80_002528"
     CDS             4695..>5119
                     /locus_tag="U4A80_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976124.1"
                     /translation="MLNRKSATITIFVLIVSVFSLSIGLNIKQELTVKTANSKIAALE
                     KKNDILKRETKDQKSRISKLEDENNKMIETQIVNKDGDVYKEFTNIATKYFKSRFNYD
                     PKTYKESKENVRDLISEELYKKFSENQLTYGDSNNVSSR"
BASE COUNT         1956 a          707 c          992 g         1464 t
ORIGIN      
        1 gagattttaa gaaattaata tctgatattt tttcacagaa gaaagaaaag taggtgtttg
       61 agtggcaatt agagtaaaag taccaaaaga aattaaggat tatcaagaaa aattaatggg
      121 aggaatgagc gttaggcagt tagtattttt tgtattggca gtattaatct cagtaggagt
      181 aacagcatat tgtgtattgg tattgcatat gtcggttgac gcagccggtt atatagttat
      241 cccactattg atacctattg tagcatttgg ctggataaga aaagaaggta tgcctttaga
      301 aaaatatggc aagattattc taaattatta ctcgaaaact ggaataagaa tatatcagac
      361 taaagaaagg agcaatctaa atgattttag aaaaaaacga ttccaaaaag tcaaagaaac
      421 aaccaaaaga taagcaagca aaaaaagtat taaaaatctt agcaaagcag aaaaaacagc
      481 gtggaaaaaa tcaacgtaga gacgctccac ctttatctag ccaacgatca ttaacatata
      541 aagcaatgta tgagaatgga gtgtgtgagc ttaaagaagg gctttattct cgtacattca
      601 aattttcaga tatcaattat gcaattgcag atcaatctga gcaagaatta acttttagct
      661 cctattgtga gttactaaat agttgtgatt ctgatacttc tttgcaaatt tcgattatta
      721 atagaagtaa agacaagaaa caatttgaaa aagacttatc ttacgaatta gttgatgatg
      781 gactagatgt atatcgaaaa gaaatgaatc atatgatatc taataagatt aaatatggga
      841 atagtagttt acaaaaagaa aaatatatta cttttaatca aagagcaaaa actttagaac
      901 aagctatacc agctttaaat agacttcaag gcgatttaga agatcaattt agcgaaatgg
      961 ggtgtgaagt agaagaactg tcaggaattg agcgtctaag ggttgtcaat gatattctac
     1021 gtcccaataa aaaacttcat tttgaatatg aggatttact tttcagtggc ttaactacac
     1081 attcagcaat tgcgccagat agttttgatt ttaaatatcg taaggatcgt ttcaagatgg
     1141 gggattatta cggtcaagtt ttgtatcttc gtaattatcc tgctgaactt tcggataaat
     1201 taattactaa tatcacagat atccctacta atttacagat tactttgcat attgagcctt
     1261 tagatcaaca aaagggatta gatttagtaa aaacaaaatt atcatttatg gagcaacaaa
     1321 agattgatga acagaaaaaa gcggctagaa gtaattatga tcctaacttg ataccaatgg
     1381 aattaagaag aagtatggac gaagcacaag aattgttgga tcaaatgcaa ggagacaatg
     1441 aaaagctctt ttcttttact tttttagtga tggcggttga taaagatgaa agaaagcttg
     1501 atgacacaat tgagcaaatt ttaactgcag gtcggaaaaa tgactgcgag tttgcaaagt
     1561 taaattatct tcaagaagaa ggtctaaact ctacgctttt atggggaaac aattttgtcc
     1621 ctatacatcg aacattaaca actgctagta ctgctgtatt tattcctttt actgcacaag
     1681 aacttatgca gccaaatggg aaatactacg gtttaaactc agtaacaaaa aacttagtga
     1741 cgttagatag gagaaaacta aaagctccta atggttttat tttaggtact ccgggtagtg
     1801 gaaaatcttt tgctactaaa aaagaaatga cagctgtttt actgagtgat cccgatgctg
     1861 aagtaatagt aattgatcct gaaagagaat atacaccttt agcagaagct ttaaatggag
     1921 aggttattca gatatccgca ggatcaaaaa atcatgtgaa tccgatggaa attaataatg
     1981 actatgcaga tgatgatgat ccagtcattt taaagactga ctttgtgtta tctctttgcg
     2041 aaatgttggt aggggggaat attggtctta gtccgactca aaaaacttta attgatcgtt
     2101 catgccgatt tgcctacaag aaatattttg aaaatgggtc tggaatgcct actttaaaag
     2161 atttttctaa tattcttaag gatcagcctg aagatgaagc aagacaatta gcagtagagc
     2221 ttgaacttta tactgtagga tcattatcag tattttccca ccaaaccaat gttgatacga
     2281 gcaaacgttt tatcgtattt gatgtaaaag atttaggaaa acaattgcga tcgatgggca
     2341 tgttgattat tctagatcaa atttggaatc gtatcacaag taatcgaaat gcaggacgtc
     2401 ggacatgggt atatattgac gaaattcagc ttcttttttc aaatgaatat gcagagaatt
     2461 acttttttga attatggtca agagcaagaa aatggggagc aattcctaca ggtatcacac
     2521 agaatgtatc tacgctccta tcttcattaa atgcagaacg tatgctatca aatagtgatt
     2581 ttgttctaat gttaaatcaa tcccaacgtg atcgagaaag actaggcgaa ttgttacaca
     2641 tttctagccg tcagaaaaaa tatattacga atgccaaaga aggtgcagga ttgatgtatg
     2701 cgggcgattc tatgattcca ctttatgatg agtttccaaa agatactaac ttatataaag
     2761 tgatgtcaac taagattgag gacgtgtata agtatcaaaa tgagcaaaga gaacaaggta
     2821 ctacagtatg aacacagatt ttcgagaacg gattatccga aaacaaaaga cagcaaaaaa
     2881 gtttgtagta aatcgagata ataatcagtt caagaaaaaa attgtatcta ctgactatac
     2941 aaaacgagct aagaaagcta atcatagtgg aaaaataaaa agtagtcggg gcacttccta
     3001 tttaataaaa aaagattcag caaaaaatgt agtcaaagat gttaactcta aagcacaaaa
     3061 aaatggtgtt gaacagggga atgcaactgc tgaattagaa acgagtaaaa caataggacg
     3121 tgggataaaa aatagcacta aggcaacaaa aacagtggct aaaactacaa ctaaagttac
     3181 taaaaaaagt aaagaattga ttcaaacaaa ggcttcaaaa aaagaagttt taactgttgt
     3241 taaaaaagga agtaaaaaag gtctcgtaaa cacaagcaaa gctgcaaaag atacagtaat
     3301 aaatgaaatc caagagttta gaggtgattc agaatctcaa gggattaatg aagcgattca
     3361 attaaaagat ttcattgata caggtatcag tggtgctaaa aaaacaaaaa aagcagcaaa
     3421 tactgctaaa aaaagtagta aagcactaac aaaatttggt aaatcaagtt acaaatatac
     3481 acaaagaatt gctgaaatag gcatacgtag agttcaacaa gttttatcaa aagaagtagc
     3541 aaaagcactc attgctaaat tagcgattcc actaacttta gcagtgctta tattatcaat
     3601 tgttacggct gtaatttcaa ctgttaacat ttttaatttc ggagcaggcg actgtgatac
     3661 tagtgccaac tatgacaata taactaccaa agatatagag tcaaatgcta aaagtattta
     3721 ctcaattatt cgtaaagaaa tacctgagtc cacccctcaa gggatttctg gagcattagg
     3781 agctatgcaa tttgaatctc aattgaatcc aagtgcagtt aatccaagct caggtgcgac
     3841 aggaattgcc cagtggttag gttctcgttt aacctcttta aaagaatttg cacacaaaaa
     3901 aggaaagaag gaaacagatt taggtgttca agtagaattt ctgttatctg aattaaatag
     3961 ttcatattat caatctagca aaaaaatatt agcaatgaca gatgttcatg aagcatgcaa
     4021 agagtgggtc atgaagtttg aaggattatc tcaagactct agccaatggt atttggatca
     4081 aagaaactct tatgctgatc attggtacgc aactttaggg acgagtgatc caattgctga
     4141 aggtactatt agtaatggtg cagatagcga attaaatacg cttacttgtg atttagaatc
     4201 tgatggtggc gatattatgg aaattgctaa gagttggcta ggttggtttc actatgatca
     4261 gatacaccct gctcctgatt taggaacaga tttaaaaaat cctaataaaa atggaagaac
     4321 tgattgctct ggttttgttt ggctagtttt aaataaagct gggtataaag tacctgcaaa
     4381 tatgcaatgg tttactggat caatggcaag tgacgctaga ggatcacacc aatatttgaa
     4441 gcaaatttca gagacagaag caaaagcagg cgatattgtt attgttaatc aaggtgctgg
     4501 agcaggtagt aatggacaca caggaatttt gactgaaaag tggagaggta aagataccaa
     4561 aatcattcaa gaaggtggaa atggcgatag tgtaaatatt gaagcttttg gtacgtcctt
     4621 tatgagctta ttagatggtg gcgatgttgt attagcacgt cctataaaaa aataattaga
     4681 ggggaggaag tactttgctc aacagaaaat cagctactat aactatattt gtattgatcg
     4741 tatcagtatt ttctttatcc attggattaa atattaagca agagttaact gtaaaaactg
     4801 caaattctaa aatagctgct ttagaaaaga aaaacgatat tttaaaaaga gaaacgaaag
     4861 accaaaagag ccgaattagt aaacttgaag atgaaaataa taaaatgatt gaaacacaaa
     4921 tagtcaataa agatggtgat gtctacaaag aatttacaaa cattgctact aaatacttta
     4981 agtcaagatt taattatgat cccaaaacat acaaagaaag taaggaaaac gtaagagatt
     5041 tgatttctga agagttgtat aagaaatttt cggagaatca gctaacttat ggagattcta
     5101 ataatgtgag ttctcgatt
//
LOCUS       DASJRQ010000131         5065 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJRQ010000131 DASJRQ010000000
VERSION     DASJRQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.135"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U4A80_002529"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U4A80_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN1976125.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="U4A80_002530"
     CDS             complement(2944..3378)
                     /locus_tag="U4A80_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976126.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="U4A80_002531"
     CDS             complement(3395..4600)
                     /locus_tag="U4A80_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1976127.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1608 a          920 c          680 g         1857 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttag
//
LOCUS       DASJRQ010000132         5009 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJRQ010000132 DASJRQ010000000
VERSION     DASJRQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.136"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U4A80_002532"
     CDS             complement(<1..182)
                     /locus_tag="U4A80_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1976128.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U4A80_002533"
     CDS             517..951
                     /locus_tag="U4A80_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1976129.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U4A80_002534"
     CDS             974..1294
                     /locus_tag="U4A80_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976130.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U4A80_002535"
     CDS             1435..1626
                     /locus_tag="U4A80_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976131.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="U4A80_002536"
     CDS             complement(1697..1954)
                     /locus_tag="U4A80_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1976132.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="U4A80_002537"
     CDS             2112..2375
                     /locus_tag="U4A80_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976133.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="U4A80_002538"
     CDS             2399..2587
                     /locus_tag="U4A80_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976134.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="U4A80_002539"
     CDS             complement(2670..2855)
                     /locus_tag="U4A80_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976135.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="U4A80_002540"
     CDS             3070..3441
                     /locus_tag="U4A80_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1976136.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="U4A80_002541"
     tRNA            complement(3591..3663)
                     /locus_tag="U4A80_002541"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="U4A80_002542"
     CDS             complement(3709..4137)
                     /locus_tag="U4A80_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976137.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="U4A80_002543"
     CDS             complement(4197..4955)
                     /locus_tag="U4A80_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1976138.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1744 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttatt
//
LOCUS       DASJRQ010000133         4908 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJRQ010000133 DASJRQ010000000
VERSION     DASJRQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.137"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..1094)
                     /locus_tag="U4A80_002544"
                     /pseudo
     CDS             complement(1..1094)
                     /locus_tag="U4A80_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249826.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1374..2339)
                     /locus_tag="U4A80_002545"
     CDS             complement(1374..2339)
                     /locus_tag="U4A80_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1976139.1"
                     /translation="MDKTGTITVGKPQVTDIISIGRISENEILRIAAGLEDSSEHPLA
                     LAVINEAKDKKITPAVAKNFTAISGKGVQALIDGKQAFIGNDRLSDDFNMTDDLKIKM
                     TSLQAQAKTVVLVGYDGQIIALIGIQDAPKSSSKAAIRAMQKSGFHTVMLTGDNRLVA
                     QAIADDIGIDEVIADVMPGDKAQHIRKLQEKGAVAFVGDGINDAPALSTATVGIAMGS
                     GSDIAIESGGIVLVKNDLMDVVTSLVLARKTYSRILINLFWAFIYNVIGIPVAAGIFS
                     ALGFTLSPELAGLAMALSSITVVLSSLLLNYVRLPKNSETLIGNS"
     gene            2461..3141
                     /locus_tag="U4A80_002546"
     CDS             2461..3141
                     /locus_tag="U4A80_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1976140.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YMNNLIEQDHRPIKRRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            3224..3841
                     /locus_tag="U4A80_002547"
     CDS             3224..3841
                     /locus_tag="U4A80_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976141.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            4005..4337
                     /locus_tag="U4A80_002548"
     CDS             4005..4337
                     /locus_tag="U4A80_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976142.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            4661..4822
                     /locus_tag="U4A80_002549"
     CDS             4661..4822
                     /locus_tag="U4A80_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEN1976143.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
BASE COUNT         1593 a          820 c          936 g         1559 t
ORIGIN      
        1 tcagcttctt acaaaaccat tgtggaaggc attgatctaa gctgttgatc gtatcgaaaa
       61 aactttggtt gtccttgtgt ttaaagtgat agaggaggag ctagcaagtc tcgtaagccc
      121 tggttaattg ctcatcgtaa ctcaaaagct catcgaccat cgctttttgc gtcattggtc
      181 tgcgaaagga tcgatcatat tgataatcag aactatccag atcataggca tgcgtaagga
      241 gtaatttcca gaatcgcttc aatcgtcaat actttgtcgg ttccgttttt tgaaacctgt
      301 tcattgtttt gattcgcaac tgatttaacg tacgagtgat ttgttggaca atgtgaaagc
      361 gatcggtcac gattttggcg tttgggaaga ccgcttttac aagttcaaaa taaggggcat
      421 tcatatccat cacgacgtat tttacgttcg ctcgtgcttt tcgagtgaaa ttaaggaaat
      481 atggttttaa gaaagtaagg cgacgattct ccaatacacc gattaattgc tgggtctgcc
      541 cattcataaa aacaaaactc attttttcca ctgcaggact tcatcgattt gtactcatca
      601 aaacaaagga ccgaaggtaa cgtatcaaaa cgaggatgat acgtttttaa acaggtgtgc
      661 aggacacgca aaacgattac gtctgataca aaacagcgat tggcaatttc tttttttatt
      721 gtattcttcg ctagttccaa ggcaatttgt acctttaatt cctttgagag atagcagttt
      781 tcatctacca aattggtctg agcgttgaat gtcgactgac attctttaca gtaaaagcgc
      841 gtacgtacca agcgtaattc tgttttggtt gctcggtaag gcaaaagttg gacttttgtt
      901 ttatgtgagc cattcttgat gatttgtcct tcatttttga tgccacattt acggcaatgg
      961 cttagtatat agctaagttt atcatggata agattggtgc gaaaaccatc ttcttctact
     1021 gtctctaacc attcttcttc gaagaataaa ttttcatctg ttaaacctaa caattttcta
     1081 gtacaatgat acatggagat atcctttctt ggtatggttt tgttttagac gactaaattc
     1141 caccagttag gctatctctt tttctatctt tttttacaaa aaaagtgtca gtagaaaatc
     1201 tgtagatttt ccaccaacac taaatattgt agaatcaaaa atcagaacta catccagtag
     1261 ttctgatttt tggttctctt attgtatgcc tagtatttaa gactctgatt caaattttga
     1321 aaaaggaaaa ttactattat aattaaaagc gtcacttttg tatattacaa atactaacta
     1381 ttacctataa gtgtctcact attttttggc aagcgcacat agttcaataa tagtgagctc
     1441 aaaacaacag taattgagct aagtgccatt gcaagaccag ctaactctgg agatagtgta
     1501 aatccaagtg ctgagaatat acccgctgcg actggaatac ctatcacgtt atatatgaaa
     1561 gcccaaaata agttaatcaa aatccgacta tatgtttttc gtgctaatac taaagaagtt
     1621 acaacatcca tcaaatcatt tttgactagt acaatacctc cagattcaat tgcaatatca
     1681 ctccccgatc ccatagcaat acctactgtt gccgtggata atgcaggggc atcattgatt
     1741 ccatcaccta caaaggcgac tgctcctttt tcttgcaact ttctaatatg ttgtgctttg
     1801 tccccaggca taacatctgc tatgacctcg tcaatcccaa tatcatctgc tatggcttgt
     1861 gcgaccaaac gattgtcccc agttagcatc acagtgtgaa atcctgattt ttgcattgcc
     1921 ctgatagcag ctttagagct ggacttaggt gcatcttgaa tcccaattaa agcaattatt
     1981 tgaccatcgt aaccaactaa taccacagtt ttcgcctgcg cctgtaaaga tgtcatttta
     2041 atcttaagat catctgtcat gttaaagtca tcggataaac gatcattacc aataaaagcc
     2101 tgcttaccat caatcaaagc ttgtacccct ttaccagaaa tagcagtgaa atttttagct
     2161 acggcaggag taattttctt gtcctttgct tcattaatta ctgctaaagc taatgggtgt
     2221 tctgatgaat cctcaagtcc agcagcgatt cgcagaatct cgttctcact aattcttccg
     2281 atagagataa tatcggtcac ttgtggtttg ccaacagtaa ttgttccagt cttatccatg
     2341 acgacggttt ttatatcgtg ggtcgcttca agaacctcag catttttggt tctgttgcaa
     2401 agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga ggacagagta
     2461 atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc tgttggttac
     2521 tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga tcgtggaata
     2581 aatgtttgtc atactacgat ttatcgttgg gtacaagagt acagcaaagt cctctattat
     2641 ctttggaaga agaaaaatag acaatccttc tattcatgga aaatggacga aacctatatc
     2701 aaaattaagg gacgttggca ttatctttat cgtgcaattg atgcggacgg cttaacctta
     2761 gatatctggt tacgaaagaa acgggatacg caagcagcct atgctttctt aaaacgactc
     2821 cataaacagt ttggtgagcc gaaagcaatt gtgaccgata aagcaccttc tcttggctcc
     2881 gcctttagaa agttacagag tgtgggttta tatactaaga cagagcaccg aactgtgaag
     2941 tatatgaaca atttaataga acaagaccat cgacctatta agcgacggaa taaattttat
     3001 cgaagtctcc ggactgcctc atccacgatt aaggggatgg agaccattcg aggaatatat
     3061 aaaaagaacc gaagaaatgg aacgctcttc ggcttttcgg tgtctactga gatcaaggta
     3121 ttaatgggaa taccagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa
     3181 cttcgcaaca gaaccattat tttaaagtgg gaggaaaatt gttttgtgga tatttaaaca
     3241 agaaatcttt gctaaattat ttagtttaga tggattaggt tggggaatct tattttttat
     3301 ttttttattt ttaggatcta tgtggatctg gtttgttgat aaaaaaaatt cttatgagaa
     3361 agtaatagtg aaaaaaatga atcgaaatga attcgatgat ttttcaaagg aacatagagt
     3421 tgaattgtac gatagtcatg aaattaacag tacttctcca tcttattatt gggtatttgg
     3481 ttgcgaagaa aaaccattgg agattaaatt gttgaaaatg cttgaaaatc ctccagtcat
     3541 tgaagatttt tctgcaaaaa acgttgaaaa acagatgttt gaacaggtac aaataagagg
     3601 tcaaaacaaa atttcattag aaccagatga atatgcagcc ttttttttag aaacaaattc
     3661 aggtacttca atgcctgttt tcaaagttaa attaaaaatg aatggtcgtt tatgcactta
     3721 tgatcttgtt gcaaatatgc gctatggttc tactggtgat agacgtacta ttttaaaatt
     3781 tgagcaaacc tttagaagtt ttttgaaaaa tagagtatcc caaattttta atttggattg
     3841 aaaaaattaa aaatttggcc aaaaatacta cagtttttta gagcaatcat cttatgatga
     3901 ttgctctttt tttgttgttt ttgtgtcatt cgttgttcaa atcctctatt cgtaaaataa
     3961 atattttaaa ctagaaatat gccttatttt taggaggaat caaaatgaac aatagagaaa
     4021 tattggcaaa agaaatggcg gatttgaaag caaaagaagc gaagattaga gcgaaacgaa
     4081 aacagattag acagaaagaa atgatttctt tagcgaaatt atattgtaaa aaggaaggct
     4141 ttaagacacc tgctgaagca ttagattttt tggaatcaaa taaaagaaat cattcagtaa
     4201 atgatgaggt tttagtatcg ctaagaaaat ttgcagatgg attagtttgg caagaaaatg
     4261 gtgcatattg gcgtacgaat gatgttaagg gtttgactca atttatggca caatttcgtt
     4321 cagaaaatgt gaactaaatt gaggaggggt tgaagtgaaa agcaatactt ttcacttttg
     4381 gttttttgcg gaataaaaat tgtgaaaaca atgacgcaaa aagccaatgg ttttgaacga
     4441 aaaaaggacg taaaaaagga atgcgaaggc agtcgttttt cgttcgcttt ttcgtttgag
     4501 agaaacggag tttctcatcg gctgttaagc cgatatagcc ctcggcagag cttacagact
     4561 ttacgcagta taagtctagt aagttatacc ttacatggag gtttttccga atttagcttg
     4621 tgattctcgc aataatttta acgaaaggaa gtacacagtt atggcctata tggtggcacg
     4681 aacacaaaag cataaagttg ggtcgttaaa tggacttcaa aatcatgtag atcgtaaaac
     4741 gaaaaatcat acgaataaag acattgatat tacgaaaagt ggttctgttg caaagttttc
     4801 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggataga gtaatgaatc
     4861 attttaaagg caaacaattc aaaaaatgac gtcattattg tcgctgtt
//
LOCUS       DASJRQ010000134         4885 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJRQ010000134 DASJRQ010000000
VERSION     DASJRQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.138"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            512..2356
                     /locus_tag="U4A80_002550"
     CDS             512..2356
                     /locus_tag="U4A80_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1976144.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2412..3878
                     /locus_tag="U4A80_002551"
     CDS             2412..3878
                     /locus_tag="U4A80_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1976145.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3915..4757
                     /locus_tag="U4A80_002552"
     CDS             3915..4757
                     /locus_tag="U4A80_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1976146.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1607 a          784 c         1029 g         1465 t
ORIGIN      
        1 aagcgctaat ctaattcact gtattatcat tttttagtag tatagcacaa ttatgaatgc
       61 cttttcttta tatcaaataa agtttatata aaaaacctca tcctgcaaga aagaaaaaat
      121 gaacccattc tttttatact aggtcttaaa aagacagaac atctgctaga agatagaaga
      181 gaataagagt tatatatgtt ggaagatatt cttagaaaaa agacaaaact tttagaacac
      241 gaaaactata ttgaacgaat aaaatgaaaa aatgattaaa aaatcatttt attatttacg
      301 tttacattct aatatgttaa attgaatata acttaaacag gcatgtgact ggtaatgcag
      361 gcaggaccta aataagttga ataacaatgt atgcgtttca atacacgttt gccttgttgt
      421 caatttattt agtttctgcc tgttttttgt gttgaggatg gccacttcga ctcaaaagtt
      481 catcactcaa aaaagaaaag agggaaacac catgaattac aaacaaacag cagaagaagt
      541 attgcagtac atcggtgggg aagaaaacat atcccatttg gagcattgct caactcgttt
      601 acgttttacg ttaaaagaca acagcaaggt aaatatgagc cagttagaaa aagtggatgg
      661 cgttatgggc gttcgccaaa atgtgcaatg ccaagtaatc attggaaacg atgttgttga
      721 agtgtatgat gaagtgaaag cgttattagg tgacaatgtt tcaaatactg aagaagcacc
      781 aaaacaaaaa caaaaattag gttcagttct attagatttt attatttcta ttttccaacc
      841 tttaattcct gcgattgcag gtggaggagt attgaagtcc ttgttgctac tagcttctat
      901 cttagggctg attagtgacc aatctcaaac ctatcagatt ttaaatttgg tcggcggggc
      961 accgctttat ttcttgccta ttctggtggc aatgactacg gctaataaat tgaaagtcaa
     1021 tcaattggtt gcagtatctg ctgtcggtgc attgatcctt cctgaaatga cagctatgtt
     1081 ggcggaaggt gctacattct tatcttttgg attggataat atagcgtatg catctcaagt
     1141 attcccagcc atcttaactg ttttatttta tgctcaactt gaaaaatggt ttacaaaagt
     1201 ttcaccaaaa ccaattcgta ttttctttgt acctatgatg agcttattga ttacagttcc
     1261 tattgctttg ttgcttctag gaccattagg ttataatgta ggaacagctt tctcttctat
     1321 cattgtgtct ttgtacaatc aatttggatg ggttgcaacc ggagtcttag cagctgtact
     1381 gccattaatg gttgtaacgg gtatgcataa agcaatgatc ccttacgctg tttcatccat
     1441 gagtgaattg ggtgcagaac ttctttatct cccggcttca ttggcacaca atatttcaga
     1501 agctggcgca tgctttgctg tcagtatcaa aacaaaagac aaaaaattga aatctacggc
     1561 tgtttctgcg ggtatatctg cattattcgg aattactgaa ccagctcttt acggggtaac
     1621 gattctgcac aaacgtgttt tatatgccgt tatgttttct agcctgatcg ggggagcatt
     1681 tgcaggaatc gttgcacttc aagcctttgc tttagtgggt cctgggattg caagtatcac
     1741 gatgttcgct gacaaagctg acccaatgaa tctggtttgg gcatttgcag tatcggggat
     1801 ttcatttgtg gttgcttttg ctgcagcgtt ggttctttat aaagataaag aagttgtcaa
     1861 tgaagttgaa actaagatgg aaacggccgt tgtttcagat gctgttcttt caagtcccgt
     1921 gaatggaaaa gtaatctcac tatcagaagt aaaagacact gttttttcta gtggtatggt
     1981 aggtgatgga gtagcaatca ttcctgaaga agggatatta gtagctcctg aagatggaga
     2041 aatcacaatg gtatttgaaa cgaagcatgc tattggttta aaaacggcta atggtgcaga
     2101 acttcttttt catattggta ttgatactgt tcagttagaa ggaaaagggt ttgaggcatt
     2161 tgttcgttct ggtgaaaaag tagccaaagg acaaaagctg attgcatttg atattgattt
     2221 aatcaaagaa gcaggattcg accccacagt tatctgtgtg gtaaccaata aggataatta
     2281 cactatccgt tcatttaacc tagaaaaatt ggtaaccaat gatagtgatc tcttagctat
     2341 cagtccgata gcttaaacac aagatagtga aaaaaaatat attgaatgaa ggaatagagg
     2401 agaatagaat aatgacaaac acaacaagtt ttccaaaagg atttttatgg ggcggcgcga
     2461 tagcagcaaa tcaagtagaa ggtgcttgga atgtagacgg taaaggtctt tcagtagcgg
     2521 atatggctac ttataaacca aatttagaca acaaagattt tgctgctcat atgcatgtca
     2581 ccctgcagtc tataaaagaa gctagtgaag acaaatctga taaatggtac ccaaaaagaa
     2641 ggggcgttga tttttatcat cactataaag aagatttagc tttatttgcc gaaatgggct
     2701 ttaaaacctt gcgcctctct attgcttgga ctcgcttgtt ccctacagga gaagagctgg
     2761 aaccgaatga aaaaggggtt gcattttatg aaaacgtctt taaagaaatg caacgtttag
     2821 gaattgagcc gattgtgaca ctatctcatt atgaaatgcc attagcttta gcagttaaat
     2881 ataatggctg ggtggagcgt cgagtaattg atgattttgt tcgcttcgct aacgtttgtt
     2941 ttgatcgctt cggcaaatac gtaaaatatt ggctgacatt taatgagata gacagcatcc
     3001 accgtcatcc atttacaaca gctggaatta tccaagagaa aagcgcagaa ggtcaagaag
     3061 ctcaagatat ctatcaagca ttgcatcatc aatttgttgc gagtgcgtta gtgacaaaag
     3121 atgctcatga aaaaattcca ggaagccaag ttggttgtat gttaacgaaa ttgatgactt
     3181 atccattaac ttgcgcacca gaagacgttg agctgacctt gaaaaagaac ttagaaaata
     3241 atttttatgc agatgttcaa gttaagggag aatatcctgc tctaatcgaa aaggatttgg
     3301 aaaatcgggg aattcagatt aaaatggaag atgaagatcg agaaatttta aagaaacata
     3361 ccgtagattt tgtttctttc agctattaca tgtccatggc agaatcagct gatccaaacg
     3421 cagaacgcac accgggaaat actgttttag gggttaagaa tcctcatttg ccttcaacag
     3481 attggggctg gcaaatcgat ccaaaaggtt tgaaaatttc gttgatcgaa ctttatgatc
     3541 gttaccaagt accgttgatg attgtggaaa atggtatggg agcagtcgat caggtagtag
     3601 atggtaaaat ccatgatccg taccgcatcg actactttaa agcacacttc caacaaatga
     3661 aagaagcaat cgatgaaggt gtagatttga tagcttatac tagctgggga ccaattgatt
     3721 tagttagtgc aggaacttct caaatgtcta aacgatatgg atttatttat gtggatgcag
     3781 acgatgaagg aaatgggaca tataaccgct tgaaaaagga ttcgttcaac tggtacaaag
     3841 aagtgatcga aacaaacggt gcatccttat tcgagtagtc tcgatctttt taagcagcga
     3901 tagggggata atgagtgatt tcaataaaaa aagtattgaa ttcaagtgtg gtacttgttg
     3961 aagaaaatgg caaagaaatg attgcattag gtaaagggat aggctttgga cagaaagctg
     4021 gggatgaaat atcaggagat aaagtagata aaatatttct gccttttgaa gaaaagaaat
     4081 cttcacaact attagagtta gttgaagaaa tacctattga attttttgaa atcactcgta
     4141 ctatcgtttt tgaagcagaa aaaggattag ataaaaaact gaatcctgga gtctatttaa
     4201 ccttaacaga ccacctgcat tttgcagttg aacgcgcaca aaaaggggta aatattgcaa
     4261 accgtttgta ctgggaaata aaaatctact atcccaaaga atttcaaatt gggaagtacg
     4321 cactcaccct tttgaacgag caatatggaa ttgatcttcc taaagaggaa gcctcaaaca
     4381 ttgctttcca tttgatcaat gcacaatcgg aagatcctga tagcggcgct ggattcaagt
     4441 atgcaaaaat gatcggcggg atcgtcaatt tggtccggta ttcgttgcag aaagatatcg
     4501 atactaattc gatccattat gcccgcttca ttacacatgt acgtttcttt gtagaaagat
     4561 attattctga tgctttgttg gatgatggtg aggaagaatt ctacaatcaa atgtggatct
     4621 tatacccgtc tgccatggaa agcgcagcta agatcaaaga ctacattgaa aaagtttatc
     4681 ataaaacaat tccgaacgat gaagttgtat acctaggtgt ccacatcaat cggttgatga
     4741 aacatgctaa tgggtgagcg ctcagtaata ctattgatgc ttttaaaatc tttagagatg
     4801 gattatagta aaaacaaggt gatttccagc tgtttgttat ctataagaga catttaaaaa
     4861 ggattaacgt aagataataa aaaat
//
LOCUS       DASJRQ010000135         4869 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJRQ010000135 DASJRQ010000000
VERSION     DASJRQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.139"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(203..1345)
                     /locus_tag="U4A80_002553"
     CDS             complement(203..1345)
                     /locus_tag="U4A80_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976147.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1350..1535)
                     /locus_tag="U4A80_002554"
     CDS             complement(1350..1535)
                     /locus_tag="U4A80_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1976148.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1889..2134)
                     /locus_tag="U4A80_002555"
     CDS             complement(1889..2134)
                     /locus_tag="U4A80_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1976149.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2128..2529)
                     /locus_tag="U4A80_002556"
     CDS             complement(2128..2529)
                     /locus_tag="U4A80_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1976150.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="U4A80_002557"
     CDS             complement(2716..4524)
                     /gene="uidA"
                     /locus_tag="U4A80_002557"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN1976151.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4547..>4869)
                     /locus_tag="U4A80_002558"
     CDS             complement(4547..>4869)
                     /locus_tag="U4A80_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1976152.1"
                     /translation="IKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIP
                     TIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYCGLK
                     DTFI"
BASE COUNT         1536 a          932 c          632 g         1769 t
ORIGIN      
        1 aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac atggtttatt ttttacactg
       61 ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg tccattttcg ataccgacag
      121 ctagcttata acccaaggta tatcaacata aaaaaataaa aataggctat gacagcagcg
      181 tgacagcaaa aaagatggaa agttaaatat caaacctcgt aatttcattt gcgagtgctt
      241 gatttgtttc tggatataag tgtccatatt tgtccaaagt agttttaata gaggcatgac
      301 ctagacgctt agatattgca tagatatttt tattatgttc aattaaaaag gcaacatgcg
      361 agtgtcttaa tccatgcata tgaattggtt ttactcctgc aatttcagct agttttttta
      421 ttctgcttaa aatagttctt ggagatggcg gtaatccatc aaaagaaaaa accaattcta
      481 tatttttccc aattgattgt tgagtttttt tccatgtttt taaatcttca acggtttgtc
      541 tgtctaacga aattatccga atacttgatg tattttttgt ttcagcaaat tcataatctt
      601 gtcttgtacg gatgtaaatt gacttattta cgctacatct gccatttatt aagtcaaagt
      661 ctttccattg taacgctaag agctcctcac tccttacacc tgtaacaaaa ataaaacgca
      721 tcaaacgttt ataaaattct tcataaaaat ccccgtgata aacactatta tatactttct
      781 taaattcatc aactgtccaa aattctactt gaggacgctg agtttttatt ttacctatag
      841 tatctacagg attttcttca ataatttcaa gtacaattgc ccttttaaaa actactctca
      901 acttattgaa tatctgttta atataatttg gtgataatgg ttgctcaatg ccctttttta
      961 caacatggat tttcgataaa aactgttgaa attcctgtat atgaattggt cgaatcttta
     1021 caacctgcat ttctccaaaa aaatccaatg cacgttgtaa aatatgatct gttttcatat
     1081 atgttttttc tacagttccc aacttatacc aaggaaaaaa atactctcga taaaaatcat
     1141 gaaacgttat tccttgcaga taaattcctt gtgaatcaga aaaactttgt tttaactctt
     1201 cgagtcccct tctagcttct ccttgagttt tgaagcctct tctggtcact tgaattcttt
     1261 ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca agtgcctctt tttctgtctt
     1321 tataaacatt cgcaattttc ctcattttac tattcctccc aagaaatatg gagaacttct
     1381 tctaaaattg aaataggaat tctttttatt tttgaattac gataaaaaaa gtatcctttc
     1441 tcaactaaaa gttctttaca ttccttgaag attttattag catagtactg agaaaaacct
     1501 agattaatca aatcttttcg agtagctgtt ttcattatag caccttcctt actttttttc
     1561 atttatttct atcagctgct tttgtcaaaa gcacatagga atttcacctc tccccaagtt
     1621 tacggcgagc cttacggaag tatcattatc ccaactttaa atcatcgctt tttattgact
     1681 ataaaaatta ttttagaata tttatcgctc cttctttaca gcaagaaatc atggcgcaac
     1741 taaaatatgc tccttaaagt gttaacgtac tgatagaatc tatttaattt tcaatgaaca
     1801 ataggttttc tacctaaaag aatctactaa ttccttaact attagaagat tcttttaaac
     1861 agaaaaagtg gaaaggggat ttttttaatt agttaatttt aaattttaag atcgcttcta
     1921 ttaatttaat ttctagctga cttatcatat attcatctac ataggtaacg aattgaccta
     1981 cctcgttaaa tccatttgtc aaacataact tagccatata agaatgataa tgtttgagta
     2041 tcatcttgat cgaaattgga tctccttcta gagcagataa gataatggat agtggaacta
     2101 ataggtactg ccgcttttta gatttatcta ccataggtca taccccattt tcaattgttc
     2161 aactgccttc tttcgttgat accagatacc acttgtgctc atattgagaa ttttaccgat
     2221 ttcagcatca ttaaaacctg caaaataata aagtaggata atctttcttc ttatctccga
     2281 taactgatca attgctgctg ataacttttg atctgaaatt aatatttcca ttccaaataa
     2341 gaaatagaca tcttgttcat caaaatgagt ttgggaatac ctgccttcat ttgaatctgc
     2401 caatggttcc tcattatcct ttatgttagc gtttcgtttt cgaatagact tagcttcgtt
     2461 tcgtaatact tttttacagt aggcatcaaa ttggtgttgt atagatatgt cattgtaatg
     2521 ttgttgcatg ataatatgct cctttttccc cctttccact aatacaacaa tcagaaagcc
     2581 cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat ttatcattct ttaaacagaa
     2641 aatctttcta taaaaaacaa aaaaaaacga ttaggctagg ctaatcgttt tttgaacatt
     2701 cttaaaaaat ccaaattatt ttttataact gaaattgggt attgatgacc atcgtttctg
     2761 tagatagtaa gcaatcattt taggttttct atctctagta aagattcctt tcttatttcc
     2821 ttgcactctt aaaatccctt gacttgttgc aaaatctgca aaattccaca cttgttcacc
     2881 aacaaaattt tcacatgtat ctattacttc gtgatttgct tcgtaataag ccacttgata
     2941 ctcttctgta aacattgttg gtactgtatc atgaaatcca gctactgtat ctgctccata
     3001 ttctgtaaac attattggcg tatcaggaca tctttccatc catccatcta gctctttttt
     3061 caacagatat tttgcatgtg ccagatcccc tcccgaaaca taccaaccat aataacgatt
     3121 taaacataaa acatcgacaa tatctcctac cttacaaaca tctggcgttg attttagata
     3181 agtaacgatt gttacaggtc ttttctgcgg atcaagttct ttagttaatt caactaaagg
     3241 ttcaaaatat tctttagcac cttcggaatc cgaatctggt tcatttgcaa tagaccacat
     3301 tacaacactt gggtggttct tgtcgcgttc tatcaattcc ttgatcaccg ttttatgaga
     3361 attttctgtt cgtatctctt tccacgtatt atgtttttcc acattatcat ctaataaagt
     3421 ggccatgaaa tttaaatgta accctactgc aggtacttca tcaataacta caataccttc
     3481 tcgatctgct aatcgcataa tttcttctga gtaaggataa tgtgatgttc taaaagaatt
     3541 agcacctatc catttcatta aattaaaatc tttaatattt acagcctcat tcaaaccacg
     3601 tccattaaca tacgaatcct catgcttacc aaatccttta aaataaaaag gtttattatt
     3661 aatcaaaaat ttgccattgg taacctgaac actgcgaata ccaaattctt ctgtataggt
     3721 atcgatcact ttattatcat caattaaact aattttcata ctatataaat aagcgtctag
     3781 aggttcccaa aaaattggat tcttaagctg aatcattcct ttctttcctt cagcttcagc
     3841 tacaagttga gcctcttcgt catatatcgc tattaagatt tttccggtcc attctgtatc
     3901 gatattgaat tctacacttg catccaaatc agttattctt gtattaatag taatatcact
     3961 tatatagtct ttcggagttg tatataactt aacaggtcta tgaatacctg cataattgaa
     4021 gaaatcaaaa ttaactaagt tttccttttt tatatcccca ttttcctgta ctgtttcctt
     4081 cataatcccc acaggtaaag tactatcgtc aataatattg tttacagcaa ctgtaattcg
     4141 attattcccg tcttttacaa accgattaat ttcagactca aatggtgtaa acccaccttt
     4201 gtgttgagta ataaactttc cattcaaaaa aacttttgct tcatgtgttg cagatccaaa
     4261 acgaagaact aatctttctt ctaataatct tttaggaaga gtcacttctt tttcatacca
     4321 cacccatcca acatgatttc taatgttctc tgtttcaaat aaatcattat aagaagaggg
     4381 aactgaaatc ggataactct ctttttcatt tattggttct aaagcaagtt cttcctgtaa
     4441 gtcatcattt gtatctaatt taaatctcca gagaccgctt aagtcaatta cttgcctcgt
     4501 ctctgtcata atgggataca acatataaac cactcctatc taaatattaa ataaatgtat
     4561 cctttaaacc acaataagca tctattggac ttactccctt aaattcagaa agacccatta
     4621 gtttgtccac caatatttct aaagtttctt gttttccatc ataagtatta atataagtct
     4681 ttacttgagg tacatcagcc aaatgaaacg gacactgtac agatacaaaa attgttggaa
     4741 tttcatgaat gtaaaacggt atatccggag ttcctttact tgctggccag acaattcgtt
     4801 gaactgtgcc tggattaaca ttagctatat taatgatcaa gtcatatttt ttagttaaat
     4861 ttttaattg
//
LOCUS       DASJRQ010000136         4826 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJRQ010000136 DASJRQ010000000
VERSION     DASJRQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.140"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="U4A80_002559"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="U4A80_002559"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1976153.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="U4A80_002560"
     CDS             2714..2938
                     /locus_tag="U4A80_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976154.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="U4A80_002561"
     CDS             2951..3451
                     /locus_tag="U4A80_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1976155.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..>4826
                     /locus_tag="U4A80_002562"
     CDS             3548..>4826
                     /locus_tag="U4A80_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN1976156.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEI"
BASE COUNT         1746 a          709 c         1024 g         1347 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaaa aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaattt
//
LOCUS       DASJRQ010000137         4733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJRQ010000137 DASJRQ010000000
VERSION     DASJRQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.141"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..1098)
                     /gene="xerS"
                     /locus_tag="U4A80_002563"
     CDS             complement(13..1098)
                     /gene="xerS"
                     /locus_tag="U4A80_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1976157.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3174..3692)
                     /locus_tag="U4A80_002564"
     CDS             complement(3174..3692)
                     /locus_tag="U4A80_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN1976158.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1554 a          778 c          718 g         1683 t
ORIGIN      
        1 tttttactac atttacaaag cgttcaaagc atcaattgta gctgcattgt ctatatgagt
       61 atataaagtt gttgtatttg tacttgtatg tcccaattgt tgggcagtta ataccaatga
      121 atttgtctgt tgatagagtc tagtagccaa agtatgacgt aatttatgtg gactgacttg
      181 gactctgaat ccttttgaat attttgctac catccgttcg attgcaatac cggatatacg
      241 cttggcttct ccttgataaa ttgctagaaa taaagctttt tcgttttctg ttactttgta
      301 tcgttctttc cgaatagcta gataattagc taaatattcc attgcaaatg ctgctatatt
      361 tactgcgtct ctcttatttc cttttctagt tactaccaca ttattctttt ctaaagatag
      421 gtcctctaaa ttaatattta caacctcgga gagtcttaat ccagatccta aaatcaatgc
      481 attgatcgcc aggtctcttt ctacatctcg ttgataatag atcaaagctc gtttgctcaa
      541 agttttttta tattcattgg caatataatc tagataattt tgtgtatctt cgtctagaaa
      601 taattttttc tcgattgcgt ttgcacgggc agctaacgta gttttatctg tgatagttgc
      661 tacttgatcc ataggattat tttctaagta tggttttcct aaaggattat tggaactttt
      721 cgaaagatat ttaaatagaa cctttaatgc tgtgatattt ctattgatag tagaagtaga
      781 tagacctaag ttattattat ctttgagctt agtttttgtt gtctcttttt ttacattttc
      841 ctttgtgcga cgtttcaaat gagatttgta caagtttaga tcataggcag ttaactgtct
      901 tatcgaggtg attgaagtat cgggaacgat agactcactc agaagccatt cataaaagag
      961 cttataattt tttagatatt ctaaagtagt tcgaggtgat tgatctttat cctcacgatc
     1021 ttgaacaaaa tcttttatat aatcgggcat atgaagtatg attgtatcta ttttttgata
     1081 tatttctgta tttttcatgt tattcatcct ttcgataagt aggggagagg agtatctcta
     1141 taattggact ataccaatta tagagtgttt tgtcaattgg tatactctga aatttattaa
     1201 tttatttcaa aaaagcagat aaataggtat agatttaata atttaaaata aatggtatac
     1261 atgaatagga aaggaaacag aaggaggcaa tcttaatttt ttaaattgtc taaattatat
     1321 agaaagaaca atctttctat ataattagac ataagagaga ctacggttga tttgatatta
     1381 acaagcgtat atgaatgtat atacaaataa aaaaggggag ggagaagtta aagaaaattc
     1441 agagaagtgg ggcactcagt agtattaaca atttaaaagg aatttcttaa agaaaatggg
     1501 ttgcatcctg gagatacaat ttttttagat aaagaaaaaa ttcaagcaac aattcaaaaa
     1561 aatcttaact gctgagattg atgtagcaat atctcaatca tcaacaaagt acgatcctgc
     1621 ctttcaaaag cttattgata aatgtttatt cattataaaa ataaaagaaa aatttatgtc
     1681 agcttttttc atcaaagtgg taatgataat tcgtctatta attgtatttt ttaggaaata
     1741 tggtataatt gttttaacga aaaggtaatt catgatacac caaaaaccac tagctggcgg
     1801 gctagtggtt ttcttttacg ttctattcag attaacgggt aggcttactg tcttttttgg
     1861 caataatttt gcaatcaagc catttacaga tgtaatgcgc ggatacttct gctaagattg
     1921 caaaaaacat atcgaaaagg taattcataa tacaacctcc tccccttgcc ataataggcc
     1981 ggggtagcag acagcataaa tattatacca taactgaaaa tttcatttta gtacatttca
     2041 atatattgat gaaccagcac aataaaacat gccatgacta acagaaaaaa acttattaaa
     2101 ccagatataa cttgtaaaca tccaactata tagaaaacgt aaattttcta tatagttgga
     2161 ttaagataac gactatttat tttatcatat ttttactata ccctatactt ttacttggta
     2221 tatttatgaa taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa
     2281 aatactaaag ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt
     2341 tagataacac gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact
     2401 caatcgaaag tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc
     2461 ttttacatct tgcagaaatc gttactggga ttaaactaag caataaaaac taatgttccc
     2521 ctcattcttt gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt
     2581 caggatcttc aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat
     2641 aggcgatagg ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat
     2701 gttggcttta gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg
     2761 ttcttaacaa tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc
     2821 ttacccttca tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc
     2881 ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt
     2941 tgtagcggat atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg
     3001 ccaaccaaaa ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact
     3061 tgcattcacc caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac
     3121 tgttaacata caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa
     3181 gctaatctct tccattttct tatctttgtt ctaaaagtcc caaatggagc aactgtattt
     3241 acatgaataa atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt
     3301 cttttgtgtg cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca
     3361 tcaatattac gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt
     3421 gtaaaccact tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta
     3481 acagataatc catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact
     3541 tgatatgcta aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat
     3601 tcttcaggaa tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt
     3661 tttaattctt ctttattttt atatgttctc attatattac ctccataaat tcctaattgt
     3721 ctcctttaat tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc
     3781 tgatatgcat tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca
     3841 tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttttgtag
     3901 taaaatcgga ctggtttgca tatcattttc ttcgtaaata tcaaaatcat gttaactact
     3961 atcaattact aataaaaact accactttaa gcgttaagaa ctgttaagtt tagttaagtg
     4021 aggatagttc acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt
     4081 atataggatt acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag
     4141 taaaaattga cgatgacacg ttccttacgg ctcatcagat gaaacagcta caaaaacgaa
     4201 atgatcaact cgaagaaaat ttaattctaa aaaagcgatt gccatattca cgcctcgctc
     4261 gacgaactat taaaggtcaa gatttacctg aagatcatcc tttttaggaa attgaagctt
     4321 tttatgttgc acaatgcatt tataatacta caatgatgtt ttcaccagat attcttctag
     4381 ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa
     4441 ttatgaataa ttatgttaat cttccgaaat ttttttccaa aattaaggga tcaagctggt
     4501 gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc
     4561 gatactctgt taagttggtt gacacggtgt cgatttttat gactttctta ttctgaaact
     4621 tcactaacgg acagtaccta aaaagtctat agtatggacg tttacgagta ctattttatc
     4681 aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact acccccactt agc
//
LOCUS       DASJRQ010000138         4698 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJRQ010000138 DASJRQ010000000
VERSION     DASJRQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.142"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..865
                     /locus_tag="U4A80_002565"
     CDS             2..865
                     /locus_tag="U4A80_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1976159.1"
                     /translation="MTDPLQNIINLQVKMQTAQVANNRLNEIFAIDSEKSNQSFEYML
                     SEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIVGVSGSGKSTLAKLFVRF
                     YEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTIIENLIFGLDSPPSFEQI
                     IEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLALARALLKNPDILILDEAT
                     SGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILVLHEGHLVEKGTHDELRF
                     ADGIYQKLWEI"
     gene            834..2249
                     /locus_tag="U4A80_002566"
     CDS             834..2249
                     /locus_tag="U4A80_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN1976160.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            2282..3316
                     /gene="sppA"
                     /locus_tag="U4A80_002567"
     CDS             2282..3316
                     /gene="sppA"
                     /locus_tag="U4A80_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1976161.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            3318..3839
                     /locus_tag="U4A80_002568"
     CDS             3318..3839
                     /locus_tag="U4A80_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1976162.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            3836..4222
                     /locus_tag="U4A80_002569"
     CDS             3836..4222
                     /locus_tag="U4A80_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1976163.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         1726 a          643 c          882 g         1447 t
ORIGIN      
        1 tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa cggctcaagt
       61 tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga accagtcttt
      121 tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa acgtttcatt
      181 ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc cttcgcacag
      241 taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa agttatttgt
      301 tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc acttagacat
      361 tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat ttttctttag
      421 tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt tcgaacaaat
      481 aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc cattacgtta
      541 cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac aacgattagc
      601 cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag caactagtgg
      661 attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa aagaaaaaac
      721 gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa ttttagttct
      781 ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg ccgatggtat
      841 ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat aataattggt
      901 tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg gtgttatacc
      961 ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa aaagaggttg
     1021 ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa gtgccattag
     1081 aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag gacgatgtgt
     1141 tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa caagaaaacc
     1201 aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata agtaaaggaa
     1261 aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag agcatgttgt
     1321 cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat caaaagcaat
     1381 tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt tcagatagta
     1441 aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca ttaaaagatt
     1501 tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat aatgtttctg
     1561 atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa caaattgagc
     1621 aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt aaaccaacta
     1681 cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta gaacaagcaa
     1741 taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag aaccagtcag
     1801 ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct gtatctggaa
     1861 cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga acagtgcttg
     1921 cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt ttacctgcga
     1981 atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat caaaaaggtg
     2041 gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct actgccactg
     2101 agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg tttccgtttc
     2161 gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat tggcaacaaa
     2221 ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag gaggagttta
     2281 tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt tactatccat
     2341 tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa ccctaacagg
     2401 aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag aagaaggcgc
     2461 atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata caggtgaaag
     2521 caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc taaagaaaat
     2581 tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg ggggaggaat
     2641 atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag atattcctat
     2701 ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg ctggttcaga
     2761 taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta tttctggttt
     2821 aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata aaagtggtgc
     2881 attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata aagttataca
     2941 ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag gaagaaatat
     3001 ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta accaagcagt
     3061 agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt ccctaaaaaa
     3121 agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa ctggctttgc
     3181 ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa ctacatcaga
     3241 tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac caatgtatta
     3301 ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct aattatgtat
     3361 ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt attagcatca
     3421 tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct gacaaatcca
     3481 ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt gtacttttga
     3541 ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga gtagtgagct
     3601 ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata tgtaggttta
     3661 tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg cccaagaaac
     3721 aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca ttagaagcta
     3781 ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga attaaatgaa
     3841 taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga ttttatgtag
     3901 tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta ctttaaatac
     3961 tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga atgttgtttt
     4021 atttggagga attaccctag ttttttcgat aatgattttc ataataggtt ttttagtaag
     4081 aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta ttattccatt
     4141 ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg cgggattgat
     4201 gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg attaatcaat
     4261 taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac atgtagataa
     4321 aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact ctctgattgt
     4381 tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta aatattcttc
     4441 actttttaac cttttatagt acttagattt aatcctatat gtttagagag ttcattggca
     4501 gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact gttcataatt
     4561 atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt aaaaataaaa
     4621 agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga aatgctttcg
     4681 caccaacatt tacctgtt
//
LOCUS       DASJRQ010000139         4671 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJRQ010000139 DASJRQ010000000
VERSION     DASJRQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.143"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..191)
                     /locus_tag="U4A80_002570"
     CDS             complement(<1..191)
                     /locus_tag="U4A80_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976164.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="U4A80_002571"
     CDS             complement(201..1016)
                     /locus_tag="U4A80_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1976165.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="U4A80_002572"
     CDS             complement(1278..1721)
                     /locus_tag="U4A80_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN1976166.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1855..2193)
                     /locus_tag="U4A80_002573"
     CDS             complement(1855..2193)
                     /locus_tag="U4A80_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1976167.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="U4A80_002574"
     CDS             complement(2181..2558)
                     /locus_tag="U4A80_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1976168.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="U4A80_002575"
     CDS             2782..3975
                     /locus_tag="U4A80_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1976169.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="U4A80_002576"
     CDS             complement(4141..4563)
                     /locus_tag="U4A80_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1976170.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1365 a          893 c          820 g         1593 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct c
//
LOCUS       DASJRQ010000140         4635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJRQ010000140 DASJRQ010000000
VERSION     DASJRQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.144"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            175..519
                     /locus_tag="U4A80_002577"
     CDS             175..519
                     /locus_tag="U4A80_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1976171.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="U4A80_002578"
     CDS             520..939
                     /locus_tag="U4A80_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN1976172.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="U4A80_002579"
     CDS             complement(1031..1381)
                     /locus_tag="U4A80_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976173.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2678)
                     /locus_tag="U4A80_002580"
     CDS             complement(1347..2678)
                     /locus_tag="U4A80_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1976174.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2934..3500
                     /locus_tag="U4A80_002581"
     CDS             2934..3500
                     /locus_tag="U4A80_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1976175.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3645..3732
                     /locus_tag="U4A80_002582"
     tRNA            3645..3732
                     /locus_tag="U4A80_002582"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3677..3679,aa:Ser,seq:cga)
     gene            4066..>4635
                     /locus_tag="U4A80_002583"
     CDS             4066..>4635
                     /locus_tag="U4A80_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN1976176.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt tctgtgaaac gattcgtttt
     2641 tgaggatgaa tataaataac gtcagtttcg ttttccatta cacaccttct ttttcatttt
     2701 ctttaactat aacttgaaat gaaagatttg gacaactatc atttaggtca aaactaaaaa
     2761 tgtaagggaa cccattgaat ttaaccaaat ttttttaatt ttgcaaaaaa aagttaaaga
     2821 agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa tgtttgcaaa aaatgctaaa
     2881 aatagggtac aataaaacaa catatactgc agtaaagaag tgggggagtt ttaatgagaa
     2941 gaaaagtcta taccaaggat caaattctaa aagctgctta tgatgttgtt gcaaaggaag
     3001 ggtttaaagg atttactgcg agaaatattg ctaaaaagat ggggatttcg acacagccga
     3061 tttatctgga attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg
     3121 aacaactaaa agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg
     3181 gtttgaacta tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg
     3241 atgaacatgg ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag
     3301 ttaaagaaga tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg
     3361 gtacatgggt agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa
     3421 cagaagaaca aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata
     3481 atcctgaaat aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct
     3541 gcaaactgtc aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa
     3601 ataggcttga catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt
     3661 ggtaatgcac cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc
     3721 ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat
     3781 aagaacggcc aaacaaaaat agcttttcct attttttcgt ttgattgggc ttattaccgc
     3841 agatgcaagc ttttgaacac cgtttgttcg gaataagggg ctgtgacaag acttttgtca
     3901 cagcccctta ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt
     3961 ttttataaat gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga
     4021 tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg aaacgatgtg taccgcaatc
     4081 acttatgcaa cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat
     4141 gaagtggtca ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta
     4201 gataaacatt atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat
     4261 gaggctacaa atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat
     4321 tataaagaat tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata
     4381 ctagggcaat gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc
     4441 aatatcgatt ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa
     4501 gaaaaatcga tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc
     4561 ggtgtgttga caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc
     4621 tcgctatcaa atgga
//
LOCUS       DASJRQ010000141         4633 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJRQ010000141 DASJRQ010000000
VERSION     DASJRQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.145"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..522)
                     /locus_tag="U4A80_002584"
     CDS             complement(52..522)
                     /locus_tag="U4A80_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1976177.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="U4A80_002585"
     CDS             complement(681..1139)
                     /locus_tag="U4A80_002585"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1976178.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="U4A80_002586"
     CDS             complement(1310..2455)
                     /locus_tag="U4A80_002586"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN1976179.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="U4A80_002587"
     CDS             complement(2468..3385)
                     /locus_tag="U4A80_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1976180.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="U4A80_002588"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="U4A80_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1976181.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..>4633
                     /locus_tag="U4A80_002589"
     CDS             4477..>4633
                     /locus_tag="U4A80_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976182.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKI"
BASE COUNT         1453 a          845 c          876 g         1459 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tat
//
LOCUS       DASJRQ010000142         4593 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJRQ010000142 DASJRQ010000000
VERSION     DASJRQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.146"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            189..2858
                     /locus_tag="U4A80_002590"
     CDS             189..2858
                     /locus_tag="U4A80_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1976183.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="U4A80_002591"
     CDS             complement(2949..3569)
                     /locus_tag="U4A80_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1976184.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="U4A80_002592"
     CDS             3683..4222
                     /locus_tag="U4A80_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976185.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="U4A80_002593"
     CDS             4219..4557
                     /locus_tag="U4A80_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEN1976186.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac att
//
LOCUS       DASJRQ010000143         4315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJRQ010000143 DASJRQ010000000
VERSION     DASJRQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.147"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            580..1548
                     /locus_tag="U4A80_002594"
     CDS             580..1548
                     /locus_tag="U4A80_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1976187.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            1559..2374
                     /locus_tag="U4A80_002595"
     CDS             1559..2374
                     /locus_tag="U4A80_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1976188.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2442..3152
                     /locus_tag="U4A80_002596"
     CDS             2442..3152
                     /locus_tag="U4A80_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1976189.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3188..>4315
                     /locus_tag="U4A80_002597"
     CDS             3188..>4315
                     /locus_tag="U4A80_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1976190.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1449 a          646 c          902 g         1318 t
ORIGIN      
        1 gtctttattt tttctgaata taactactgt atcaaaaaca atcgattatg agacaaaaca
       61 aaatgacctc ttttatttaa gaaaggaggt cattttttgt atcataaatt gtattaaaat
      121 caatgtatca ctttttaatc aattttaaga ccataaattg cgtactaaaa gaaattctgc
      181 aattcaaaat aaagcatttt aagccatttt caaatttaag tgcacccatc tacccgtcaa
      241 atcactttat tttccactcc agtagtttcc taattcaact gtgtgcaaaa cgatttcttc
      301 taaacaaatc aagacaaact tataaacctt catcccagca ccataattat ttaaatcata
      361 ggcatttttc gaaattagta tatgtactca agtttcactg cagattattt atatttttta
      421 gagaaataaa tttttcctat tgacaaacac aaagaaagcg gatacaatac aattatgtca
      481 catgacgtta tatgacgtta tgtaatatct ttacatttaa tcagttactt ttattttgga
      541 taaaattaac atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt
      601 aacgcaattg tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc
      661 ggttcgctgg tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt
      721 attacaggct tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat
      781 aaatctgttg tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga
      841 gaagtcgctc aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat
      901 ttattaaagg tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc
      961 aataatccta tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca
     1021 tttgaacatt acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat
     1081 ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca
     1141 atgttttata tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc
     1201 tctttgatgg aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat
     1261 ggaccttttg aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc
     1321 actcgtgctt tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa
     1381 atcgttgatg caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc
     1441 aatcccattt tattctacag tattctttca gaatatcgtt cagctttagc agatgcacgc
     1501 aatcatgatt tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat
     1561 gaaggtatta ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta
     1621 tcctggagga aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag
     1681 ctttatggga gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaaa
     1741 aataggactg gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt
     1801 gaagattgat gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga
     1861 atacccgatt cagcttactg aagaagacag agaatattta aaccagtttg aattgattca
     1921 tatgggatta tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa
     1981 gatttcttat gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa
     2041 agttgatttt ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt
     2101 gatgaagcat ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc
     2161 tattggttat gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga
     2221 tacaatggca gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga
     2281 cgatatagtc accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga
     2341 cggttcgttc ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat
     2401 aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac
     2461 aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt
     2521 atcccgagaa tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc
     2581 gaatcacagt gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac
     2641 aagggaaagg aacttttgtc aaccggcata aagtttttcg taaaattgaa tatgttatgg
     2701 gtttcagtga gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca
     2761 agatcattcc agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg
     2821 ttttatatat ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt
     2881 atttttctta tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg
     2941 aagtacttaa agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg
     3001 tcacagctga tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct
     3061 ttatggatac tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt
     3121 acctgggaga agtatatcgt ttttacttat gatttcctgt tttgatttca aggagtagat
     3181 tttatttatg aagcaagtat tgcggtcaaa aatgattttt gatggagagc agccaccttt
     3241 caaaggttat attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta
     3301 cagtgatcta ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc
     3361 agcaattcat gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga
     3421 tttaagcgat acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca
     3481 taaaaaagca gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga
     3541 cccatcagaa gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa
     3601 aaatcgcagc cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa
     3661 agaaaccagt gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct
     3721 tttagcagag gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat
     3781 gaaagaaatc gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact
     3841 tagtaaccta tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta
     3901 taaggaaaaa gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg
     3961 tgttgttgcg gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc
     4021 cgagattgac tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc
     4081 ttgctgtttg acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga
     4141 atttggaaag cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac
     4201 tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat
     4261 tctcggcttg aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DASJRQ010000144         4315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJRQ010000144 DASJRQ010000000
VERSION     DASJRQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.148"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(673..1491)
                     /locus_tag="U4A80_002598"
     CDS             complement(673..1491)
                     /locus_tag="U4A80_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1976191.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="U4A80_002599"
     CDS             1652..2341
                     /locus_tag="U4A80_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1976192.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="U4A80_002600"
     CDS             2355..3857
                     /locus_tag="U4A80_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1976193.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..>4315
                     /locus_tag="U4A80_002601"
     CDS             3870..>4315
                     /locus_tag="U4A80_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1976194.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIA"
BASE COUNT         1435 a          628 c          764 g         1488 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctca
//
LOCUS       DASJRQ010000145         4293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJRQ010000145 DASJRQ010000000
VERSION     DASJRQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.149"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(255..542)
                     /locus_tag="U4A80_002602"
     CDS             complement(255..542)
                     /locus_tag="U4A80_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1976195.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(562..786)
                     /locus_tag="U4A80_002603"
     CDS             complement(562..786)
                     /locus_tag="U4A80_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1976196.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1241..1411)
                     /locus_tag="U4A80_002604"
     CDS             complement(1241..1411)
                     /locus_tag="U4A80_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976197.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1426..1959)
                     /locus_tag="U4A80_002605"
     CDS             complement(1426..1959)
                     /locus_tag="U4A80_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1976198.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1980..2924)
                     /locus_tag="U4A80_002606"
     CDS             complement(1980..2924)
                     /locus_tag="U4A80_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN1976199.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3561..3647
                     /locus_tag="U4A80_002607"
     CDS             3561..3647
                     /locus_tag="U4A80_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1976200.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3768..>4293)
                     /locus_tag="U4A80_002608"
     CDS             complement(3768..>4293)
                     /locus_tag="U4A80_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976201.1"
                     /translation="GLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEE
                     FTTCYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKD
                     TQTEEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNY
                     LKSVKEWLDQQQQN"
BASE COUNT         1195 a          782 c          658 g         1658 t
ORIGIN      
        1 ccccccactc tccacaatac aaaaaagact agcaaatgct agccactttt tgtaaagttt
       61 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
      121 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      181 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      241 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      301 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      361 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      421 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      481 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      541 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      601 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      661 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      721 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      781 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      841 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      901 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
      961 aatgtcgact agaccaagta gcccctctac caatggatag agacgctcac tttgtgattt
     1021 ttcattggtc taggtcaaaa gcttttttta aggcttggtt ttctgcggtt gtttctttct
     1081 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1141 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1201 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1261 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1321 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1381 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1441 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1501 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1561 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1621 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1681 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1741 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1801 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1861 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1921 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     1981 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     2041 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2101 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2161 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2221 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2281 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2341 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2401 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2461 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2521 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2581 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2641 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2701 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2761 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2821 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2881 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     2941 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     3001 atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca
     3061 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3121 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3181 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3241 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3301 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3361 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3421 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3481 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3541 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3601 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3661 gacatcaacc tgaaaaaaag ccccagagaa tccaacctct ctggggcttt cgtgtatcta
     3721 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3781 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3841 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3901 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     3961 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     4021 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4081 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4141 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4201 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4261 cgtttatttc cctctaaaac gccgaataaa cca
//
LOCUS       DASJRQ010000146         4162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJRQ010000146 DASJRQ010000000
VERSION     DASJRQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.150"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..509
                     /locus_tag="U4A80_002609"
     CDS             <1..509
                     /locus_tag="U4A80_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEN1976202.1"
                     /translation="YKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVE
                     KLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEID
                     EHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNL
                     VKSYLKMN"
     gene            512..1408
                     /locus_tag="U4A80_002610"
     CDS             512..1408
                     /locus_tag="U4A80_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1976203.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1458..1847
                     /locus_tag="U4A80_002611"
     CDS             1458..1847
                     /locus_tag="U4A80_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1976204.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1834..>4162
                     /locus_tag="U4A80_002612"
     CDS             1834..>4162
                     /locus_tag="U4A80_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1976205.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1426 a          645 c          876 g         1215 t
ORIGIN      
        1 agtataaaaa tgagattgaa acatgtgtaa acgaaataag aaaattaaat tgggatattg
       61 tttctgtgaa aaaagaaata caagataagg cgaaggtaga gcttgaaatt gaagatttaa
      121 aaaaacaagt agaaaagcta tctcataaga gttataaaga tgtttatgat gagtattcaa
      181 aagagacaga tatttatagg gaactgaaac gtgatgtgga ggcacttaag gaaattccca
      241 aaaatctaac tgatagtgtt gacaaaattg actttggcaa tggtataaaa gttacagacg
      301 aaattgatga acaccgcgga gaattgatta agaatttagt gaccaatcgg gctaagattc
      361 aaacaataat taatgaaatg tcagatgaaa ttgatagtta tagagcagag ctgaataaat
      421 cagattggaa agttaactat aatgaggttt caaagaggta tcaaagaggt atcaaaaact
      481 tagtgaagag ttatctcaag atgaattaaa aatgttacaa aaccttgatt ctttatatca
      541 atctttatca aaaactgaag agcgattgga agaaattatt caacaagaaa taaagcttaa
      601 agattaccaa gttgaggttg actcagcaat gtcaagaatg aataaatcat atcaaaatct
      661 ctattctgag agaagtagtt cctcgcgcga aattttttct gatattacag gaatagaggc
      721 tactattcaa ccaaataggg atttcaataa ttatgttaaa gagctcagag cttgtattaa
      781 taaggaggac acttttgaca gtgagtttgg atctcttatt gataagctga caaaaaattc
      841 cattactaat caagatatat ataatgacat acttgatata agagttagtg gggaaagtag
      901 tattttttca gataaaaaat taatcaattc tataagaaaa ttgaatgatg cacaagttct
      961 tgaattacaa acccttaagc caaatgattt agtagttatc cgacttgaac taaatggaaa
     1021 aaaagttagt ttatcgaatg ctagtgctgg tcaaaagaca tctgcaattc tttctatgat
     1081 tttggcgtat ggaaattcac cactcatatt ggatcagcct gaagatgatt tggatagtca
     1141 attaattaat aacttaatcg taagaagtat cattacaaaa aaggaaaaca gacaaatttt
     1201 aattgtaact cataatgcaa atatcccagt taatggtgat tcagaatggt tgattagcat
     1261 gggagattca aaagaaatat cggctgatca gtgcgggtcg gtagatgaac ccaatatcaa
     1321 gccaagaata tgtactgtga tggaaggtgg cgaggatgct tttacaaata gagcgaaacg
     1381 ctatggtttt aaacaaattg tagaataaca ctagagggtg agacatatta ttatgtccgc
     1441 cctttttagg aggaaaaatg aaaaaaataa aaagctatac cagtatctgg gcagttgaaa
     1501 aagtcattta cgcaatcaat gattttcagt tgccttttcc tgtgactttt aaccaaatgg
     1561 cttggttcgt tttatcttta ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta
     1621 tgatagagga ggtcttttta aaatacttag gcataccggt ggctgtcact tggtttatgt
     1681 cgcaaaagac gtttgatggc aagaaaccac tgggattttt acgatccttt atcagctatc
     1741 acttgcgatt taaggtgact tttgcaggaa agaaagtcaa agaacagaag aaacgatggg
     1801 atgaacccag tactcttgta aggagtgtga actatgtatc cgattaagta tattgaaaat
     1861 aatctcgtct ttaaccaaga aggcgagtgt tttgcctact atgaattagt gccttacaat
     1921 tactcctttt tgtcacccga acagaaattt caagtgcatg ataactttcg ccaactaatt
     1981 gcgcaaaatc gtgaaggcaa gatccatgcg ttacaaattg ccacagaaag tagtattcgc
     2041 gccacacaag aacgatcaaa aaaagaaatt accggtagac tcaaagaagt agcgaaacaa
     2101 cgaattgata tacagacaga tgcattagta tccatgattg gcgatagtca gattgattat
     2161 cgctttttta ttggctttaa actaattgcg acagatgaag aagtcaatct aaagagtctc
     2221 aagaaatctt tcttttctgg gcttcaagaa tttgtctatg gagtgaatca tcatttgatg
     2281 ggcgattttg tttccttatc caatgaagaa attcgctgct attctaagct tgaaaaatta
     2341 atggaaagca aattggctcg aagatttaaa gtcaggcgag tcacaccgag tgacctgacg
     2401 tatttgattg aacatattta tggcgaaaaa gggataccgt ttgaagaata cgaatttcag
     2461 ctaccaaaga aaaagctaaa atcagaaaca ctggtgaaac gttatgactt attacgtcca
     2521 aatcgttgtt taattgaaga aaaaccaagg tgcttacgga tggaacatga gaatcatgag
     2581 tcgtacgtag cgtatttaac aattaataca attgtggggg agatggaatt tccttcctca
     2641 gaacttttct attaccagca acaacaattt acattcccaa tcgacacaag tatgaatgta
     2701 gagattgtga cgaataaaaa ggctttagct accgttcgta ataagaaaaa agaactgaaa
     2761 gatttagaca atcatgctta ccaatctgac aatgaaacga attccaatgt cttagatgct
     2821 ttggattccg tggatgaatt ggaaaccacc ttggatcagt caaaagaatc tatgtacaaa
     2881 ctgagttatg tggttcgagt gagtgccgaa tcagtggatg aactaaagcg acgctgtgat
     2941 gaagtgctcg atttttatga tgataccaat gtaaaactag tccggccctt tggcgatatg
     3001 atggggctac atgaagaatt cttaccctca agtaaacgat atatgaacga ctatattcag
     3061 tatgttacgt ctgatttttt ggcggggctt ggttttgggg ctactcagat gctaggagaa
     3121 ttagaaggga tttactttgg ctacaatgtg gatacaggtc gtaatgttta cctgaaaccg
     3181 gcattggctt cacaaggagt caagggttca gtaaccaatg cgctggccgc cgctttcctg
     3241 ggttctcttg gtggaggtaa atcctttagt aacaatctct tagtctacta tgctgttttg
     3301 ttcggtggtc aagctgtgat tgtcgatcca aaaggggagc gaggagggtg gaaagaaacc
     3361 ttgccagaaa ttgcggatga aatcagtatc ctcaacttga cgagtaagtc agaaaatcaa
     3421 ggtctgcttg atccttatgt gattatgaag aaaaagaaag actcggaaag tttagcaatt
     3481 gatattctga cttttttaac aggaatttca agtcgagacg gggagaagtt tcctgtgtta
     3541 cgtaaagcca ttcgaacggt gactcaaagt gatcaacggg ggttattaca agttattaac
     3601 gaattgcgaa aagacgggtc accaatcgcg gaaaatatcg ccgatcatat cgagtccttt
     3661 accgattatg attttgctca tttgcttttc tcagacggct cgattaccca atcgattagt
     3721 ttagataaac agctaaatat cattcaagtg gcggatttgg ttttaccgga tgcagaaacg
     3781 acttttgaag aatataccac catggaattg cttagtgttt cgatgctaat cgtcatttcc
     3841 acttttgcct tggactttat ccatagtaac cgagaaatct tcaaaattgt cgatttggat
     3901 gaggcttgga gttttcttca agtggctcaa ggaaaaacgt tatcgaataa gcttgttcgt
     3961 gctggtcggg ccatgaatgc tggggtctat ttcgtcactc aaaatagcga cgatttatta
     4021 gatgaacgtt taaaaaataa tatcgggttg aagtttgctt ttcgcagtac cgatattcat
     4081 gaaatcaaga aaaccttgga attctttggt ttggatcagg aggatcaaag caatcaaaaa
     4141 cgcctgcgag atttggaaaa cg
//
LOCUS       DASJRQ010000147         4159 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJRQ010000147 DASJRQ010000000
VERSION     DASJRQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.151"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..568
                     /locus_tag="U4A80_002613"
     CDS             <1..568
                     /locus_tag="U4A80_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976206.1"
                     /translation="KLIENWSVKNNIAEDDLKIFEEKVSGKNIENRPELQELISFIRE
                     RDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLPSFNGVTDPALRDLLTNLVI
                     EVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYHANATGKNKLIYDEIVDGLK
                     KKETISKIAKNAGVTRKTVYRVKRDLGM"
     gene            836..2083
                     /locus_tag="U4A80_002614"
     CDS             836..2083
                     /locus_tag="U4A80_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1976207.1"
                     /translation="MKNKLTLLICATILSTWAVPTQNAFAEETGNDTTQQTVTSNSSS
                     EEQTAIVNGEEVKLEAPVKEGQSEAEATQAPKEELQQDQMSRFARSAVRTIPMYRMYN
                     PNSGEHFYTQDTGERDNLRRIGWNYEGIGWQAPTSGDPVYRLYNRYNGEHFYTLNAKE
                     RDNITKQGWTYEGIGWYSYKGANGIPVSRLYNKRVNWHHYTLDEHEKNVISKQGWNYE
                     GVGWYAVGNGQPSNDDYKLLGVKNYNQYALGAPSGCEGASLLQGLQYKGKITNWSLTQ
                     FLNTIPKSPNGNPNNGFVGSPFVENSWTYSAIYPAPLATWGQKYGNVQNISGSSMNTL
                     LNEVKNGNPVVAWVTINFQPIRWGNWSFGVAANNNHAVTLDGYNKGSNQVHVSDPISG
                     SYWLNRTTFENIYNARKYAVVVR"
     gene            2355..3680
                     /locus_tag="U4A80_002615"
     CDS             2355..3680
                     /locus_tag="U4A80_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1976208.1"
                     /translation="MFDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMKLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQERIKEELGLIA
                     AVGVGDNPLLAKLALDNEAKNNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIPPEPSKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIIHASSKLEGARSITRSTLVGGHAGGVGGLKND"
     gene            3673..4023
                     /locus_tag="U4A80_002616"
     CDS             3673..4023
                     /locus_tag="U4A80_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976209.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDAENEQRANQNLAKEQMTTE
                     EITEVLNAAVIKNRSVCIQKEERNAEGYYPDDIIGKIFGSDELGIYVGKNKVHYDEIR
                     HIEFYQKHKWSCLE"
BASE COUNT         1527 a          631 c          852 g         1149 t
ORIGIN      
        1 taagctaatt gaaaactggt cagtcaaaaa taatattgct gaagacgatt taaaaatttt
       61 cgaggaaaaa gtctctggaa aaaatattga aaacagacca gagctgcaag agcttatttc
      121 tttcatacgt gaacgagata cgattattat tcctagtctt gatcgattag caagaaatag
      181 taaagataca aaagacttac ttcaattgtt tcgtagtaaa ggtgcttcta ttgaaatatt
      241 agacctaccg tcgtttaatg gggtaactga ccctgcatta agagacttgc taacaaattt
      301 agtcatagaa gtgtttagct atgttgctga aaacgaacgg aaaaaaatca aagagcgtca
      361 aaaacaaggc attgaaattg caaagaaaaa tggtcgttat actggtaaaa agattcaata
      421 ccatgccaat gcgacaggaa aaaacaaatt gatttacgat gaaattgttg atggtctgaa
      481 gaaaaaagaa actatttcta aaatagcaaa gaatgcaggg gttacaagaa aaactgttta
      541 tcgagtaaaa agagatttag gaatgtaata agttccagtt agaaagaagc gtaaataatg
      601 caagaaatag aaatcggtaa aatttattat tgcgaagttc ctagtttgga aggttctttt
      661 gaagttcaag ttttacaaat gctaaaagac aactgtgttt tagtgagagt cgtacgttgt
      721 acattaatgc aaagtaaata tttaaagaaa agcaaatatc gttttattgt caatatcaag
      781 tccctaaaag gacgatcgtt acacttttaa taaagagatg gaggactaaa taaaaatgaa
      841 gaataaactg acattattaa tttgtgcaac cattttgtca acgtgggcag tccctacaca
      901 aaatgctttt gcagaagaaa cagggaatga tactacgcag caaacagtga ctagcaatag
      961 ttctagcgaa gagcaaacag caattgtaaa tggagaagaa gtaaaattag aagcgccagt
     1021 gaaagaagga cagtctgaag ctgaagcgac tcaagcacca aaagaagagt tacaacaaga
     1081 tcaaatgagt cggtttgctc gtagtgcagt aagaacaata cctatgtatc gcatgtataa
     1141 tccaaatagc ggagaacact tctacactca agatactgga gaacgagata atctaagaag
     1201 aattggttgg aattacgaag gtattggttg gcaagctcca acaagtggcg atccagttta
     1261 tcgcttatat aatcgctata atggggaaca tttctatact ttaaatgcca aagaacgtga
     1321 taatatcaca aaacaagggt ggacgtatga aggaattggc tggtactctt ataaaggtgc
     1381 taatggtatt ccagttagtc gcttatataa taaacgagta aattggcacc actacacact
     1441 agatgaacat gagaaaaacg tgattagtaa gcaaggttgg aattatgaag gcgtaggttg
     1501 gtacgcagtc ggcaatggac aaccaagcaa tgacgactat aagcttcttg gcgtaaagaa
     1561 ttacaatcaa tatgctttag gcgctccaag tggctgtgaa ggggcttcat tgctacaagg
     1621 attacagtat aaaggaaaga tcacaaattg gagtttaact caatttttaa acacgattcc
     1681 taaatctcca aatggtaatc caaacaatgg atttgttggt agtccattcg ttgaaaattc
     1741 ttggacttac tcagctattt atcctgctcc attagcaact tgggggcaaa aatatggaaa
     1801 tgtgcagaat atttcaggta gttctatgaa tacattgctt aatgaagtaa agaacggaaa
     1861 tcctgtggtt gcatgggtaa ctatcaattt ccaaccaatt cgttggggaa attggagttt
     1921 tggtgtagca gctaataata atcacgcagt gacacttgat ggatacaata aaggaagtaa
     1981 tcaagtacat gtatctgatc caattagtgg ttcttattgg ctgaatcgaa caacatttga
     2041 aaatatttac aacgcacgaa aatatgcagt tgtagtcaga taacattgta tgcttcaaga
     2101 tcatttgtta atattaagga acggaactcc caaaaattcc gtactctaat ttgcagtctc
     2161 tcactcattt agagcgagag tttttgttaa attttcaaat tatttaataa agtgatacaa
     2221 tgagttgatc atacaaatca aaagaagaag cagggtaaga gtttacctac gcttcttttt
     2281 ttgtgtgttt agctatttct tctttacaat atcgaacata cgtttgtata atgtaaaaag
     2341 aaggggtgtt attcatgttt gattattcga aagaaccagt aaatgattat tttctaatcg
     2401 acatgaagtc tttttatgcg agtgtcgaat gtatagaaag aaatttagat ccattaacaa
     2461 cagaacttgt tgttatgagt cgagctgaca atactggttc aggattgata ttagcttctt
     2521 ctcctgaagc aaaaaagcgg tatggtatta caaatgtgag tagaccacgt gatttaccac
     2581 aaccatttcc taaaacacta cacgttgttc caccacgtat gaaactatat atcaagcgaa
     2641 atatgcaggt aaataatatt ttcagaagat atgtggctga tgaagatcta ctgatttact
     2701 cgatcgatga atcaatctta aaagtgaccc gatcactgaa tctttttacg actgaaggaa
     2761 cacgaagtca gcgaaggaag aagctcgctc aaatgatcca agaacgtatt aaagaagagc
     2821 taggattgat tgctgcagta ggtgtcggag ataatccctt gttagccaaa cttgccttag
     2881 acaacgaagc gaagaacaat gaaggattta ttgccgaatg gacgtatcaa aatgttcctg
     2941 ataaagtatg gaatattcct gaaatgacag acttttgggg tatcggttct cgaatgaaaa
     3001 aaaggctcaa tcaaatgggg atcatgagta tcaaagattt agctaattgg aatccataca
     3061 ccattaagag ccgtttaggc gtgattgggt tacagctgta cttccatgca aatggtattg
     3121 atcgaactga catagcaatc ccaccagaac cgtcaaagga aaaatcttat ggcaattcac
     3181 aagtgttacc acgagattat actagaagaa atgaaatcga actggtcgta aaagaaatgg
     3241 ctgagcaagt agcaatccgt attaggcaac accactgtaa aacaggttgt gttcatttac
     3301 acattggaac gtcgatctta gaaacgagac cagggttttc acaccagatg aagataccta
     3361 tcacagataa tacaagagag ctacaagact attgtctgtt cttatttaat aaatactatg
     3421 aggggcaaga agtccgacac gtcggaataa cctatagcaa gctagtatac actgattcgc
     3481 ttcagttaga tttgtttagt gatccgcaaa aacagattga tgacgaaaaa ctagataaaa
     3541 taatagataa aattaggcaa aaatatggtt ttacgtcaat tatacacgct tcttctaagc
     3601 ttgaaggtgc tagaagtatt acaagaagca cgttagttgg tggtcatgca ggcggcgtag
     3661 gagggcttaa aaatgattga ttacaatgat cgtggacgtt taaagtgggc aggattctat
     3721 ttatcagatc atactgaaga aatcgatgct gagaatgaac agcgagccaa tcaaaactta
     3781 gcaaaagaac aaatgacgac tgaagagatt acagaagtat tgaacgctgc agtcataaaa
     3841 aatagatcag tatgtattca aaaagaagaa cgaaatgctg aaggatacta tccagatgat
     3901 attatcggta aaatttttgg aagtgatgag ctaggaatct atgtcggaaa aaacaaagtt
     3961 cactacgatg aaatacgtca tattgaattt taccagaaac ataaatggag ctgtttagaa
     4021 tgaacattat aaagcaatat gagcttaagt atataacact tgaaaatctt tcagatgaaa
     4081 tctggggata tggtcaacaa ctaattaatg aaataggaat cgactgtttt aagttttacg
     4141 tattaataga tggtggata
//
LOCUS       DASJRQ010000148         4108 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJRQ010000148 DASJRQ010000000
VERSION     DASJRQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.152"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..357
                     /locus_tag="U4A80_002617"
     CDS             88..357
                     /locus_tag="U4A80_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976210.1"
                     /translation="MQTFTQHELEIISGHKQITGSNANKLNVIKSEVGEMLSMLKSDL
                     GKQYIEDDEQRHYTLKNYQSVYDKLEKMSEDEFKQIDLSGIEELE"
     gene            592..1005
                     /gene="mobC"
                     /locus_tag="U4A80_002618"
     CDS             592..1005
                     /gene="mobC"
                     /locus_tag="U4A80_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN1976211.1"
                     /translation="MEQKLAKTPENILKIANTYDDSERTFRKETRFSEREIFLLNYKM
                     SQANTRNFSQYARIMCLSGLMITTDFSDLKNLTTELGRIGNNINQLAHRANISNSVSK
                     EDFLELKTNYDQLTKLINEELLFRMRRARDNQRFI"
     gene            1005..3299
                     /locus_tag="U4A80_002619"
     CDS             1005..3299
                     /locus_tag="U4A80_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1976212.1"
                     /translation="MAVTKIHQIKTTLNKAIGYITNPEKTNNGMLVSSYNCQSQFAEV
                     EMQMTREYAREVKGDYRKVGGSEVLAHHLIQSFSPEDKVTPELAHELGRQLIDELTEG
                     KFEYVIATHIDQEHIHNHIIFNSVSFYDLKKFRSQPYRTANKIKEISNRICAEHDLSL
                     SPKKEKLKDSYKSYSNRRNNTSFRSEIRKRLIMCLNESTSWEEFEEKADHLGVNIDRK
                     GKHISYALLSEGQQRRTRGDKLDDLETFTESNISQKLSANQSYINQLKEAIEETFFSS
                     DNLEDFSFLLEQEHGITFQLNKQDQFVFHFNDVNDFKWNESLLPDSYQIKNLKDYFRE
                     SYEFTNDSSLNSIEERYREKEKSVIVQEDTPIQLQEIQIEKATKEGLLVSIEHDDLNG
                     LVFVPNNYVDVNEETGEYTIWINDKFDYTLTDKQTNEQTSFTVKGEGIIRGLEKMQGI
                     QPQEIKLSSKNILSMSNRGVSISIPELSIKRLFIPSEYVVIDEISKSCSILIGEKWNY
                     YAEPLPENDDNSNKKLPYQKFTGRSLISSLKETPELLDVFLKPKFDQLHYQSRKRYTN
                     QLVEALNTIREEKIVSASQLEQRLNEIVEQKNQAKEKIAEIDGKIKNYNNVAKLLVTY
                     EKYLPIMNEIERATMLQKIKLEQKYKEEVRQFKFAEKKLLASDNLRPDLTKEKIISVA
                     KNQEKQRKRLIEGYKHYENKLNRLIDVQEILQELDRNGLFDLSGYRLIDREEKLTDKI
                     EKYEKEVEETKQKNAKEKRKEHEI"
     gene            3416..3628
                     /locus_tag="U4A80_002620"
     CDS             3416..3628
                     /locus_tag="U4A80_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976213.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT         1614 a          562 c          743 g         1189 t
ORIGIN      
        1 tgaaaaggga attaataatt ttccttgtac aagtatcaaa gaatccatga aattaataga
       61 aatgtaaaag agagggacat ataaatcatg caaacattta ctcaacacga attagaaatc
      121 attagcggac acaaacagat tacaggttca aatgcaaaca aattaaacgt cataaaaagc
      181 gaagttggag aaatgctttc aatgttaaaa agtgatttag gaaaacaata tattgaagat
      241 gatgaacaac gacattacac attaaaaaat tatcagtctg tttatgataa actcgaaaaa
      301 atgagtgaag atgaatttaa gcaaattgat ctaagcggta ttgaagaatt ggaatagaaa
      361 cggggattgg ggcagcgccc caaaatgaaa aaagaaggat tcgcgcaagc ggatttttct
      421 tttttttgtg caagttaccg gtaacttgca aatcttgctg taagaccgcc cccctgaaat
      481 cttcgatttc taatcggagg gcggtcacac agtgaaaatt ttcgttttta aacaaagata
      541 aaacactaaa ctagtgaaaa aaacaaaaaa tattggtaag gagggggaaa catggagcaa
      601 aaactagcta aaactcctga aaatattcta aaaatagcca atacttatga tgattctgaa
      661 agaacgttta ggaaagaaac acgtttttca gaaagagaaa tatttctact aaattataaa
      721 atgagtcaag caaatacaag aaatttttcc caatacgctc gtattatgtg tttaagtggt
      781 ttaatgatta caacagattt ttctgattta aaaaatttaa cgacagaatt aggacgaatt
      841 ggaaataata tcaatcaatt agctcacaga gctaatattt caaacagtgt ttcaaaagaa
      901 gactttttag aattaaaaac aaattatgat caattgacta aattaattaa cgaagaatta
      961 ctttttagaa tgcgacgtgc tagagataac cagaggttta tttaatggca gtgacaaaaa
     1021 tacaccaaat aaaaactact ttaaataaag caattggcta tattacaaat ccagaaaaaa
     1081 cgaataatgg aatgctggtt agtagttata attgtcaaag tcaatttgca gaagttgaaa
     1141 tgcaaatgac tagggaatat gcacgtgaag taaaaggcga ttatcgaaaa gtcggtggga
     1201 gtgaggtttt agcgcaccac ttaatccaaa gtttttcgcc tgaagacaaa gtgacaccag
     1261 agcttgctca tgaattagga agacaattga ttgatgaatt gactgaagga aagtttgaat
     1321 atgtgattgc cactcacatc gatcaagaac atattcataa tcacattatt tttaacagtg
     1381 tttcttttta tgatctgaaa aaattcagaa gtcaacctta tcgaacagca aataaaatta
     1441 aagaaattag taatcgtatt tgtgctgaac atgatttatc tttatcaccg aaaaaagaaa
     1501 aattaaaaga ttcctacaaa tcttattcta acagaagaaa taacacgagc tttcgttctg
     1561 aaattcgtaa acgattgatc atgtgcttaa atgaatcaac ttcatgggag gaatttgaag
     1621 aaaaagcgga tcatttaggg gtaaatattg atcgaaaagg gaaacatatt tcgtatgctt
     1681 tattatccga aggacaacaa agacggacta gaggggacaa gttagatgac ttagaaacat
     1741 ttacagaaag taatatttct caaaaacttt ctgctaatca atcctacatt aatcagttga
     1801 aagaagcgat tgaagagacc ttcttttcta gtgacaactt ggaagatttt agttttcttt
     1861 tggaacaaga gcatggaatt acctttcagt taaataaaca agatcaattt gttttccatt
     1921 tcaatgatgt aaatgatttt aagtggaatg aaagcttgct gccagatagt tatcagatta
     1981 aaaatctaaa agattatttt agagaatcct atgagtttac gaatgactct tccttaaatt
     2041 caattgaaga aagatatcgt gaaaaagaaa aaagtgtgat tgttcaagaa gatacgccta
     2101 ttcaattaca agaaattcag atagagaaag cgaccaaaga aggattgtta gtgtctatcg
     2161 aacatgacga cttaaatggt ttagtgtttg tcccaaataa ttatgttgat gtaaatgaag
     2221 aaactggaga atatacaatt tggatcaatg acaaatttga ttatacactt actgataaac
     2281 agacaaacga acaaactagt tttactgtaa aaggggaagg aatcattcgt ggactggaaa
     2341 aaatgcaagg gattcagcca caagagataa aactttctag caaaaatatt ttatctatga
     2401 gtaatcgtgg tgtatctatt tcgataccag aactgagtat caagcggctc tttattccta
     2461 gtgagtatgt tgtaatcgat gaaattagta aatcttgttc tatcttaatt ggggaaaagt
     2521 ggaattacta tgcagaacca ctccctgaga acgatgataa ttcaaataaa aaattacctt
     2581 atcaaaaatt tactggacgt tcattaatca gtagtttaaa agaaactcct gaattacttg
     2641 atgtgtttct aaaacctaaa tttgatcagc tacattatca atcaaggaaa cgatatacta
     2701 atcaattagt cgaagcttta aatacgattc gtgaagaaaa aatagtttca gcttcacaat
     2761 tagaacagag attaaatgaa attgttgagc aaaaaaacca agcaaaagaa aaaatagctg
     2821 agattgatgg aaaaataaaa aattataaca atgtcgcaaa acttttagta acttacgaaa
     2881 aatatttacc gatcatgaat gaaattgagc gtgctacaat gcttcaaaaa attaaattag
     2941 aacaaaagta caaagaagaa gtacgccaat ttaaatttgc tgaaaagaaa ttactagctt
     3001 cagataatct acgtcctgat ttaacgaaag aaaagataat atctgtggcg aaaaatcaag
     3061 agaaacagag gaaacgactt atagagggct ataagcatta tgaaaataaa ttaaatagat
     3121 tgattgatgt tcaggaaata ttgcaagaac tagatagaaa tggattattt gatcttagtg
     3181 ggtatcgttt gattgataga gaagaaaagc taacggacaa gatcgagaag tatgaaaaag
     3241 aagtagaaga aactaaacaa aaaaatgcaa aagagaagag gaaggaacac gaaatataaa
     3301 taactaaaaa cactatatat tccgaaaagt gagtatctcc attcataaaa taatcaacat
     3361 atttaaaaaa tctaaagttc tttattctat gatataatgt tactgtagaa agtttgtggc
     3421 ggtttggcaa atccaattga aggagcgtgg tgcctatggg cttattagct tatcggacgt
     3481 actctgtaga aaggaaatgc ctgatttgtt ctcattggat caaatcattg ctattatcgg
     3541 agcagcagca gtagtaatta ctgccgtagc gaaagctagc gattcgattt tagcttggtt
     3601 aaagttcaaa aaagaacaaa aaaaatagag ccgtcctaac ttttggcaga gtagacaggc
     3661 tctaaagctt tatatacatg ccatccgctt tgaacggact acacattagg ggctatgttc
     3721 gcgcatagct ccttatttac ttatagtata acgtagatag agaaaaaaga aaagaaataa
     3781 aggctagttt tgttttgata aaagctaacc tttatttttt tgttcacatt ttaaatattt
     3841 gagaaagaat taaaggaggt ggtgtcgtta gcaacgacta caacttgaaa ccagctttct
     3901 attaaactat ctctttttat gtatcttttg ggtatactct aatggtacaa atccatattt
     3961 gcttaaagcg ttgatttttc agcgtttttt ctttattgca aagtgtaccc taagacctgt
     4021 atactaatag taaatagatt ggataggaat gatggcaaat gtatttagct tatttaagag
     4081 tttcttccga cgatcaaagt ttagctcg
//
LOCUS       DASJRQ010000149         4083 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJRQ010000149 DASJRQ010000000
VERSION     DASJRQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.153"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..605)
                     /locus_tag="U4A80_002621"
     CDS             complement(75..605)
                     /locus_tag="U4A80_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1976214.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(787..1443)
                     /locus_tag="U4A80_002622"
     CDS             complement(787..1443)
                     /locus_tag="U4A80_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEN1976215.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1570..2043)
                     /gene="dfrF"
                     /locus_tag="U4A80_002623"
     CDS             complement(1570..2043)
                     /gene="dfrF"
                     /locus_tag="U4A80_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEN1976216.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2438..3829)
                     /gene="amt"
                     /locus_tag="U4A80_002624"
     CDS             complement(2438..3829)
                     /gene="amt"
                     /locus_tag="U4A80_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1976217.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
BASE COUNT         1279 a          871 c          584 g         1349 t
ORIGIN      
        1 aaaatcttaa ggattttccc gccaacgtgg tcggatcgct ccgtcggttt aacagctttt
       61 ctgtttctct ctagttatct acgcatataa atcaaatcgg ttattccgtt ataagtctgt
      121 gttttacaaa gccttaactt tatttccctg tctatgttgc ccaaaagacg aaccccattt
      181 cctaacagag taggaataac agaaatgtaa tatttatcaa tcatattttt gcttaccaac
      241 tgctgaacaa gatttgctcc gccacaaatc caaatatcct ttcctgcttc ttgttttagc
      301 cgttccaata ataaaacagg attttcactt gtaaaatgaa tttgttggga agaattgcac
      361 tctttatgtg ttaatacata agttgtaaat ttattatata cccattcttg cggagaaagt
      421 tcagtaacaa cctgatgaga agtattccac cccattaaga cagtatctat atctttggta
      481 aattctgtat aagtgtcgat gttttcattg tcatttccat gaccatttag ccaagctact
      541 ccaccattac cgtccgcaat atatccgtca aggctcatgg caataaataa aactactttt
      601 ctcatttttt ctcttttata aatacaaaca ggaatttatt tctctttttt actcataagg
      661 gaacaactga ttgctacaaa taaacccatt aaaatccatg gaaatgctgc tcttataaaa
      721 tcactaacca tactttattc ctccttaaat tccaatttcc ttagttctac aaactgaaat
      781 ttttgcctat gaatgtatct tgttctttat acgcaaacag gcttctgtgt gcataatcca
      841 atgtcttgaa aacggcattt gaattagtcc acaaatatct ttattacacc aataatcaat
      901 cagccaaatt gctttttcat tgtcgtttac tacattcaac gattctaagt atccttttct
      961 ttctttcggt atttttcttt tcaattcatc ataagataac cgttcaagca ttttctcagt
     1021 gctttcccat acttcgaaaa tatataaata taattcttcc agattaagct gttttgaaaa
     1081 gtctgcaatc tgctgtttca tgagttcatt tcctgttgta atgataggtg aattgatacg
     1141 ctcttgataa ttgcctgcaa aaaatacttg ttcatcttca tttatcactg tatgcacaac
     1201 tatatcttca atgcggaaaa tatgccaaat agaataagca atcgtcttac tatgataacc
     1261 atcagcgttt atgaagggga ttgagttaaa atcttctctg cataattctt ctttgaaaga
     1321 tactaaggtt tgtattaact gacttcgtaa agtaagtaaa gtatcaatac ccctcttata
     1381 agtatctttc ttttttattt gtgcctgcat tgttttatta agttcagacc attccttatt
     1441 catattcaat cctctctcaa attctgattt tatcgcccca tacaaactgg aatttgtctt
     1501 acaacaattt atatatgcat aaaaagcaat atatttatga aaacacccta aatccaaaat
     1561 ttactcaatt cattttttcc ttacataaaa tgttctcgta tatttcactt cctcaccaag
     1621 tgtttcccct atcaaaactt caaaatcatt gatatcaaat tctggaaaga atgtatctcc
     1681 atcttcaata tttaaatcta cttctgtgat atacatttta tctactattt gcaaagcttc
     1741 cttaaatagt ccatatccac cagatatgta tacatctcgt cctttagcca ataataatgc
     1801 atcttctaat gatttaactg aaactaaatt atctccttga tactctgttg tggtggaaac
     1861 aacaatattc attctattag gcaacggatg accgatttct tcataagact ttcgccccat
     1921 aataaccaca ttacccgttg ttaactctct aaattgcttt tgttctccct ttattttcca
     1981 tggtatatta ccattcttgc ctataacatt attctttgac ctcgcaacaa tcaaacctat
     2041 catctatttc atcctccata atatgatgtc gtacctgtga ttccatcaag tgcaccacat
     2101 cttaacaaac tcaaaattat ctcgaactaa ccagctcgac aaattccttt tttgtcttca
     2161 tctgcattat atcatattgt tataagcatt gccatttctt ttctgctttt gaaaagttac
     2221 ttatcagcct atgttttgta aagggtgata ttcagcattt ctcacgcaac aagcccttga
     2281 caaaccatag actaataagt tttaaggtaa tcaagcagaa atgaaatagt ctgctgtttc
     2341 acggcaacaa aaaagagata ccaacacact tatgtagtat ctcttagtct agtagcaata
     2401 ttcttttgtt tgcaagcagg taaacatggg ctgtttctta atatcttttt gtttgggaaa
     2461 cacctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     2521 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     2581 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaaga
     2641 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     2701 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     2761 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     2821 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     2881 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     2941 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     3001 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     3061 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     3121 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     3181 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     3241 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     3301 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     3361 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     3421 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     3481 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     3541 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     3601 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     3661 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     3721 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     3781 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     3841 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     3901 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     3961 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     4021 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     4081 tat
//
LOCUS       DASJRQ010000150         4074 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJRQ010000150 DASJRQ010000000
VERSION     DASJRQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.154"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            558..3197
                     /locus_tag="U4A80_002625"
     CDS             558..3197
                     /locus_tag="U4A80_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1976218.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="U4A80_002626"
     CDS             complement(3365..4063)
                     /locus_tag="U4A80_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1976219.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1231 a          781 c          904 g         1158 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccct
//
LOCUS       DASJRQ010000151         4057 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJRQ010000151 DASJRQ010000000
VERSION     DASJRQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.155"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(166..1671)
                     /locus_tag="U4A80_002627"
     CDS             complement(166..1671)
                     /locus_tag="U4A80_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1976220.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1668..2354)
                     /locus_tag="U4A80_002628"
     CDS             complement(1668..2354)
                     /locus_tag="U4A80_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1976221.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2547..3968)
                     /gene="gndA"
                     /locus_tag="U4A80_002629"
     CDS             complement(2547..3968)
                     /gene="gndA"
                     /locus_tag="U4A80_002629"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1976222.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          857 c          693 g         1391 t
ORIGIN      
        1 ttgttttttc gttcatctaa aagatactta catttaatta acggatgtta ttctatttaa
       61 ttgttcgcta gtttttttat aatgacatcc ctgaatttct cacttgcttg tattttgtac
      121 cttattcttt gttggatata taaaaaaaca agtaaagcac atgctttact tgtcttctgc
      181 ctttgcgata ggaataaaga ttctaaaaat tgtcccttgc cctactacac tttctgctaa
      241 gatacgtcct ttatagtttt caaccagttg tttagcgata gacaacccca gaccatttcc
      301 acccttcgtt ctagctcttg cattatccac tcgataaaaa cgattgaaga tcttattaag
      361 gtcttctttg ggtattcctt cgccaaagtc ctgaatcgcg atctcaaatt cattcagagt
      421 ggaagaaata gagatatgga cttcttttcg tgtagttgaa tactttactg cgttatccaa
      481 tatgataatg attaattgtt caaagtgatt acgataaatt tgcagggtca cttcttttgt
      541 caaatcatca tctaaagtga tcacatagtc tggatataat atcttgaaat tattataaac
      601 ttgataggtg acttcttttc ctttagttgt ttcattcgca taatgaacat ctacttgttc
      661 tgctcttgat aagtctagca tctcttgaac taagcttttc atacggctga tttcttgcat
      721 gctggcgctt aatgattctt ctaatatctc aggatcatct ttcccccaac gatttagcag
      781 actaagatgc ccttcaataa ttgcaacagg tgtccttaac tcatgagata cgtcttccac
      841 gaattgctct tgttgttcaa tatacaaacg catacggtca agcatctcat tgaatatttc
      901 agctaaatct gccaattcat catttgtgtc gatttcaggc atgtggatat ctgattgcgg
      961 atctttacgt atagtatcca ttgtatctct gagtaccttg agtggtttta agaaataaga
     1021 agataaaaag aaacctaaaa tactacttaa gatcaaagaa acaacttcta aaacaataag
     1081 agtcaataat agcttatttc gaatatcata gaaagaagat aattcataga agctttgcgc
     1141 atagccgatc ttttctcttg tacctttaga atatataggc tggatagata aaaagccagt
     1201 ttttccttct agtgtaacga tcgttggaac tttacgtgta gtctgaatca ggtttcgact
     1261 gttttcatgg gttttaaaaa tcaagttctc gtccaaatca taaacataaa gatccattga
     1321 tggctgccct agctcagcta taaaggaatc gatcttcatt aagtttcctt cgaccagtga
     1381 agtatgatct acgacctcat ctccgtcatt tgtcgctttc gtcagattac gatacgtatt
     1441 tactaatgtc agttcttcat ccgagttagc caaacgagaa gctacttctg aaatcgtacg
     1501 ctctacattg tttctttctt ttgccacaat cagattgata gatgatttat aggtaatgac
     1561 agcgaaaatt gtaaacacaa cgaatatgaa gaaagaactc gcaaaggccc acttgattgt
     1621 caaggagggc cctttcagtt ctcttttttc cgattttttc ggctttatca cgagcgcatc
     1681 acataacctg ttccgcgtac agtttggata tagctttcct ccccgggaac gtcaattttg
     1741 ttgcgaagat aacgaatata tacgtccact acgtttgttt caacttctgt ttcgtatccc
     1801 cacactttat ttagtaaaac gtcacgtgct aataccacgt tcacgttttc cattaaagta
     1861 agcagcaact cgtattctct ttttgttagt tcaattactt catttccgcg acgtacgaca
     1921 cggttttctt tttcgatagt caaatcgcga taagtaatag tagtttgttt agccacattt
     1981 ttatcgcctt caatatcgat tcgacgcaac aatgcacgaa gacgtgctaa taattcttcg
     2041 atagcaaatg gtttgacgat gtagtcatct gctccgtggt caagtccaga tacacgatcg
     2101 atcacagaat ctcttgcagt catcataatg attggtgtat ttttgacttg gcgtacacgt
     2161 ctgcagactt ctaaaccgtt caattctggg agcatcagat ccaacaatat ggcatcccac
     2221 tcattattta atgccgcatc taaaccagtt cggccgttgt agtggacttc tgcgttatac
     2281 ccttcatgct tcaactctag ctcgacaaat cgtgccaagt tcttttcatc ttcaatgatt
     2341 agaatattgc tcatttatta ttttatcctt tctattctac tggcttttta aaaactctat
     2401 cattataccg ataatatgct taaaaatcta tagtcatttt atcatggata gcaattttaa
     2461 aaaagagctg aagtctactc ataacaatcg acttcagctc tttcgtcctc tttttatttt
     2521 ctcatttcct aacagccgaa agactattat tcttcatggt accatgagta atggaagatt
     2581 ccttctttgt cgacacgctc ataggtatgt gcgccaaagt aatcgcgttg ggcttggatt
     2641 atatttgcag gcagacgttc tgctctgtat gaatcgaagt aagcgatggc tgatgagaaa
     2701 gtcggtacag gtacgccagc ttggacagcc aaggcaacga cgtctctgac tgattgctga
     2761 tattttttcg tgatttcaac gaagtaatca tctagcaata aattttcgat ctcaggattt
     2821 ttgtcatagg catccgtaat tttttgcaag aatcgagctc ggatgataca tccagcgcgc
     2881 cagatttttg cgatttcacc aaatggcaag tcccaatcaa attcttttga agcgacacgt
     2941 aattgagcaa atccttgggc ataactcatg attttactga aatacaacgc ttctcggatt
     3001 ttttcgacca attctttttt gtctcccgtg aactcaaaat cgttcgtacg agacaaaatc
     3061 ttgcttgctt gtacgcgttc ttctttataa gcagaaatat agcgggcaaa tacagattca
     3121 gtgatcaatg gaagtggcac acctaagtca agcgcgcttt ggcttgtcca tttacctgtt
     3181 cctttattcc cagctgcatc aaggatcaca tctactactg gtttacctgt tccttcatcg
     3241 tctttacgtg tcagaatgtc agcagtgatt tctatcaaat agctgtctaa ttcgccttga
     3301 ttccattctt taaagatatc agccatttct tctactgaaa ggtttaaaat attcttcatt
     3361 aagtcataag attcagcaat cagttgcata tctccatact caataccgtt gtgtaccatt
     3421 ttcacataat gtccggctcc atttgggcca atatatgtga cacatggtgc tccgtcttct
     3481 gcttttgcag aaatttgctc taagattggt gcaactagcg cataggcttc tttttgtcca
     3541 ccaggcatga ttgaaggtcc ttttaaagct ccttcttcac caccggaaac gcctgtaccg
     3601 ataaagttga tgccagagtt ggctaactct tcattacgac gcatcgtatc tttaaagaat
     3661 gtatttcctc cgtcaatcaa aatatcgcct ttatcaaggt gaggaagtaa ctcttggatt
     3721 gttgcatctg ttgccggtcc tgctttgacc attagcaaga tccgacgtgg tttctcgata
     3781 gattctacaa attcttcgat tgtatatgtt gctttgaaat ttttatctgg gtgttcttct
     3841 actacagcgg ttgttttagc ccctgtacga ttgaataaag ccactgaata gccgcggctt
     3901 tctatgttca aggctagatt tttacccata acggccatac cgacaacgcc gatttgttgt
     3961 tttgacatat gaactcctcc tacatacggg atttctccct aatctcttac attatagctt
     4021 aacacggcta cattgcaaac taaagaacaa catattt
//
LOCUS       DASJRQ010000152         4036 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJRQ010000152 DASJRQ010000000
VERSION     DASJRQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.156"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..392)
                     /locus_tag="U4A80_002630"
     CDS             complement(243..392)
                     /locus_tag="U4A80_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976223.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="U4A80_002631"
     CDS             854..1066
                     /locus_tag="U4A80_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976224.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="U4A80_002632"
     CDS             complement(1344..3143)
                     /locus_tag="U4A80_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1976225.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="U4A80_002633"
     CDS             complement(3267..3863)
                     /locus_tag="U4A80_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1976226.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg tttttt
//
LOCUS       DASJRQ010000153         4022 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJRQ010000153 DASJRQ010000000
VERSION     DASJRQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.157"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            86..1435
                     /locus_tag="U4A80_002634"
     CDS             86..1435
                     /locus_tag="U4A80_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1976227.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="U4A80_002635"
     CDS             complement(1563..2153)
                     /locus_tag="U4A80_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1976228.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="U4A80_002636"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="U4A80_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1976229.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1152 a          741 c          784 g         1345 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 at
//
LOCUS       DASJRQ010000154         4015 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJRQ010000154 DASJRQ010000000
VERSION     DASJRQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.158"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            467..1960
                     /locus_tag="U4A80_002637"
     CDS             467..1960
                     /locus_tag="U4A80_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1976230.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2095..2391
                     /locus_tag="U4A80_002638"
     CDS             2095..2391
                     /locus_tag="U4A80_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1976231.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2492..3343
                     /locus_tag="U4A80_002639"
     CDS             2492..3343
                     /locus_tag="U4A80_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976232.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3364..3647
                     /locus_tag="U4A80_002640"
                     /pseudo
     CDS             3364..3647
                     /locus_tag="U4A80_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1428 a          581 c          816 g         1190 t
ORIGIN      
        1 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
       61 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
      121 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
      181 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
      241 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
      301 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
      361 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
      421 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
      481 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
      541 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
      601 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
      661 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
      721 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
      781 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
      841 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
      901 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
      961 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     1021 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     1081 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     1141 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     1201 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     1261 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     1321 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     1381 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     1441 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     1501 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     1561 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     1621 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     1681 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     1741 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     1801 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     1861 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     1921 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     1981 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     2041 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     2101 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     2161 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     2221 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     2281 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     2341 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     2401 tttacttaaa aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg
     2461 ttaaaggaaa gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac
     2521 ctggcttcct ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc
     2581 attaggaaaa ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat
     2641 tggagtattt ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga
     2701 catttttaca gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt
     2761 agtcatgcaa tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga
     2821 tttaattgtt gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag
     2881 aacaacaaaa ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat
     2941 gtatttgttt gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat
     3001 taaacgttca attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga
     3061 gtacaatgtt gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag
     3121 tatccatgaa tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa
     3181 agcaatagat cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata
     3241 tggcatcgga aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt
     3301 ttggaacagt agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg
     3361 aaaatgattc ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag
     3421 aaggagcagt tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt
     3481 acgatgaaca ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
     3541 ttgattcgaa agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg
     3601 atgaatggga acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg
     3661 taaagagcat aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc
     3721 tattacgttt ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg
     3781 atagtgaagg atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc
     3841 tatccttttt aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa
     3901 atcgtaagag aaattgatcg aagatgtgac gaacggttct gttgcaaagt tttaaatcta
     3961 ctatcaaata aggtagaata atagaaaaag atagcaggag gaatgacgat gaatc
//
LOCUS       DASJRQ010000155         3925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJRQ010000155 DASJRQ010000000
VERSION     DASJRQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.159"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="U4A80_002641"
     CDS             complement(573..1130)
                     /locus_tag="U4A80_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976233.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="U4A80_002642"
     CDS             complement(1309..1920)
                     /locus_tag="U4A80_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976234.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="U4A80_002643"
     CDS             complement(2033..2290)
                     /locus_tag="U4A80_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976235.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="U4A80_002644"
     CDS             complement(2887..3165)
                     /locus_tag="U4A80_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976236.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="U4A80_002645"
     CDS             complement(3199..3450)
                     /locus_tag="U4A80_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976237.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DASJRQ010000156         3914 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJRQ010000156 DASJRQ010000000
VERSION     DASJRQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.160"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..407
                     /locus_tag="U4A80_002646"
     CDS             <1..407
                     /locus_tag="U4A80_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976238.1"
                     /translation="AEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMI
                     LTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEY
                     WTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(533..1210)
                     /gene="nth"
                     /locus_tag="U4A80_002647"
     CDS             complement(533..1210)
                     /gene="nth"
                     /locus_tag="U4A80_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1976239.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1217..1915)
                     /locus_tag="U4A80_002648"
     CDS             complement(1217..1915)
                     /locus_tag="U4A80_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1976240.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2183..3367
                     /locus_tag="U4A80_002649"
     CDS             2183..3367
                     /locus_tag="U4A80_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1976241.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3373..3816)
                     /locus_tag="U4A80_002650"
     CDS             complement(3373..3816)
                     /locus_tag="U4A80_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1976242.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1021 a          768 c          641 g         1484 t
ORIGIN      
        1 aagctgaaaa attttgttta ttattaaata aatttacttt aattgctcct atccttgcat
       61 ttattatttt ttctgtactt ttaagcacta ctcttaaagg aaaatttatg gaaagaagct
      121 ctcatgcaat gattctcact tttttatggt tgttgttcac cagaatctat atttttctga
      181 tgagcctgaa accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat
      241 tatcacttat catttttatt acacctttag atagatacgt cacttattta tataatcctt
      301 tggaatactg gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt
      361 ttcccaataa aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc
      421 tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc
      481 tccataataa caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc
      541 gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata
      601 ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatctaccaa
      661 aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg
      721 tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac
      781 gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat
      841 ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt
      901 gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc
      961 gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg
     1021 catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg
     1081 tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt
     1141 ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt
     1201 tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt
     1261 cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt
     1321 cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt
     1381 caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa
     1441 tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag
     1501 aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt
     1561 gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga
     1621 tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga
     1681 taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca
     1741 tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca
     1801 actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta
     1861 aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt
     1921 ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt
     1981 tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc
     2041 atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc
     2101 cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat
     2161 tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg
     2221 atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt
     2281 tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat
     2341 gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca
     2401 ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct
     2461 ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac
     2521 ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag
     2581 aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat
     2641 tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt
     2701 tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa
     2761 acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt
     2821 caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg
     2881 ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt
     2941 ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat
     3001 gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga
     3061 aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt
     3121 atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca
     3181 actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat
     3241 acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc
     3301 atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc actacaataa
     3361 aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa
     3421 tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca
     3481 tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa
     3541 atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag
     3601 tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt
     3661 cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa
     3721 tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat tagctaaatc
     3781 cagatattct tctattagca gttgtctttt atccatttct acaccttctt tttctttcat
     3841 tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg aaaacccaat
     3901 ctgaaaccaa gaaa
//
LOCUS       DASJRQ010000157         3711 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJRQ010000157 DASJRQ010000000
VERSION     DASJRQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.162"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..1111)
                     /locus_tag="U4A80_002651"
     CDS             complement(407..1111)
                     /locus_tag="U4A80_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976243.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1298..1543)
                     /locus_tag="U4A80_002652"
     CDS             complement(1298..1543)
                     /locus_tag="U4A80_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976244.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1560..2411)
                     /locus_tag="U4A80_002653"
     CDS             complement(1560..2411)
                     /locus_tag="U4A80_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1976245.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2577..3581
                     /locus_tag="U4A80_002654"
     CDS             2577..3581
                     /locus_tag="U4A80_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1976246.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1158 a          632 c          720 g         1201 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccg cttttttctc tatcagaaaa tatgtttatt attagaattt
      181 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
      241 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
      301 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
      361 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
      421 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
      481 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
      541 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
      601 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
      661 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
      721 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
      781 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
      841 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
      901 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
      961 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     1021 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     1081 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     1141 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     1201 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aaaaatggaa
     1261 cggattatgt atatatacat aatccgttcc attctttcta ttttttcggt atcaaggcaa
     1321 aaacaggtag ttcaggtgaa taaatgactt catgttcgtg ccagggtttg accattagcg
     1381 aatgatcgaa ccgttttttc agttcttcga tgatttgatc atgtgtccag tttcttgcag
     1441 gaaaatgttg gattttctct gcatcgatca aaaagacaaa atcggaatga aaagtctggg
     1501 taacttcttc cgagagagaa ttcaagcgtt cgattggatc cataaaatca cctttcgact
     1561 taaaattggg tagtatctgg gtcttttgct ttgccgacta caccatctaa ctgatcgatt
     1621 tgtttcattt cttcttctgt taattcaaaa tcaaataatt cagtattttc tttgattctg
     1681 cttggtgtca ctgacttagg aagaggaagg aattcgtgtt gcagcgacca acgcaaagca
     1741 acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt tcatttcagg cacatcgaag
     1801 attttaccag tgcctaatgg actatatgct tcgaggatca tatcatgttt ttttgcatac
     1861 tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa cttgattgac catcggcata
     1921 atttttgctg tttttgctag tgtatctaga tggtggggta agaaattgct cacaccgatt
     1981 gctttgattt tacctgcttc atacagttct tcaaatgctc gccatgtatc tgcatttgct
     2041 tgttcccaat tatcacgaaa agcaacaggg tttggccagt ggatcaaaaa cagatctaaa
     2101 taatctgttt ggagcttctt caatgattct tcaaaagaag acatcacaag ttcatagctg
     2161 tggtttgcat tccataattt tgttgtcaag aatatctctt ctctaggaat tccactttcc
     2221 ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt gggcagtatc gatatggcga
     2281 taacctgcag ccagcgcttc tttaacagag gaaacagcga catccccatc aggtgtttgc
     2341 catgtgccga atcctactac cgggatttcg taaccatttg atagacgata agtgcttgtt
     2401 aatgaattca tactttcagc tcctttttta ttcgttcaac tataagtgta gcgcattttt
     2461 cgaaaaaaag aatccatttt gtttctactc ttgaaatttt tgtttaaaag aaaatgattt
     2521 tgattctggt atttttgatt ttttttccgt aaactaagac agaagggaat gagaaaatgg
     2581 atatctattt aaaaaaagca attctgcata ttattgacag agaaactggt tctccagtct
     2641 tttcacaaaa agagctggat ctgacaaaag aatatattcg tgattttctt caaaagaaga
     2701 tccaaaaaat ctcttctgct caaacaaaaa ctggacaact agcagaagaa agcatgatcg
     2761 ttgaatcact taaaatcgct caagaaaatt ttgtggaagc aagtgaagca atcgttcagc
     2821 ggtggttcga tatctaccaa gaaagtgaag aagcacctag tgcagatgta tttgttgtct
     2881 tgtatgaatt agatacagtg atgtacttat ctttattgaa agtcaattat cgtgaggctt
     2941 acactcactt tgttgaagca gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag
     3001 caattctagg ggcgaaaaca caaaaagcag atgaagcatt cgctgtgaat ttgactgatt
     3061 ggacctacga attaattgaa aaaaaatatg agttttctgg caaaaaagag ctttattttt
     3121 ctggaagagt gatcgaaagt gttcctgcac cttctttaga agaaaatgta aaagtgatcc
     3181 aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga agaatttgat atcatggctg
     3241 atttgaaaga agctgtctat gatacgatag aagaaaaagg tcggttggat catgagatga
     3301 ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac
     3361 aagagcaagg atttgtccca gaagcacctc ctgtaaaaga agtacaagaa atttctgaga
     3421 aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat cgaactgatc gtaccagtag
     3481 atgtatatcg caacccagat ttgattgaat ttgtgaataa tccggatggt acgatatccg
     3541 tgatgatcaa aaatgtggat gaagtattga atcgtttata aaaatgacta gaatattagg
     3601 tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga
     3661 atataacaaa aaaagagcgt gagacaaaag taaagaacac ttttgtctca c
//
LOCUS       DASJRQ010000158         3678 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJRQ010000158 DASJRQ010000000
VERSION     DASJRQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.163"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..445
                     /locus_tag="U4A80_002655"
     CDS             <1..445
                     /locus_tag="U4A80_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEN1976247.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            445..1644
                     /locus_tag="U4A80_002656"
     CDS             445..1644
                     /locus_tag="U4A80_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1976248.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1660..2304
                     /locus_tag="U4A80_002657"
     CDS             1660..2304
                     /locus_tag="U4A80_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976249.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2315..3121
                     /locus_tag="U4A80_002658"
     CDS             2315..3121
                     /locus_tag="U4A80_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976250.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3143..3373
                     /locus_tag="U4A80_002659"
     CDS             3143..3373
                     /locus_tag="U4A80_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976251.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1360 a          574 c          765 g          979 t
ORIGIN      
        1 taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga ttgatgagtg
       61 ccataatgcg attaatccag attttattga aacggtaaat tatgtcacaa actttcaacg
      121 agaaatgcgt aaagtagaag caatgactgt tttagctaca caatccccac aagaaatggt
      181 gccagaaaat atgtcttctg ataacttctc gaaactgaag aaagtctttg aattttcttc
      241 tacgaagatc tttatgcgta tggatgaatc tattttacgt catatagaag gattagtggg
      301 taaatcaatg acgaactcag aattagaatc cattcctcaa caaaatcaag gagacgctgt
      361 gatcaatttt ggctcaaaag aaaccattcg tgttcatttt agtccgaaca aacgtcaatt
      421 aaaattgttt gatggaggaa aataatggaa aaccaaaatg aaagtttaat caaacagtat
      481 gttaagcgtc gtgcaaagcg gcgcttattt ttgtgggtgt ttggtacttc tgcaggtttg
      541 attactatat tgatcactgt gtttgtgacc ctttttctaa tacttgcggc aggttctatt
      601 gataattctg attcaagtag tggtggcgaa gcttttacag gagaatattc ggaaggctta
      661 cccatttata aagaaatcaa aggaagaggc cctttttctg atgagattgc gcaatatgct
      721 gtaggtgcag cagtgaagta taaattatta ccaagtgtga tcctttcgca atacggttat
      781 gaatcagcgt ttggtacgtc ggcgtccgca agaaatgatc tcaattattt tgggattacg
      841 tggtttgatg ggtgtctgtt tcctaaagga actgcgcgtg gaattggtgg cattgaaggt
      901 ggctggtaca tgaagtttcc caactcaaaa gctgccttct cctattacgg ctttatggta
      961 gccacacaat caaattttaa tgcttgtgtc ggaaataaaa gccctggcgc aagcttattg
     1021 atcttaggac gaggagggta tgccgccgca ggaattactg aagacagtgc ctattataaa
     1081 aattgtatgt ctattattaa tacaaataaa ttgatggagt acgatgagtt tgcgattaaa
     1141 cactggagag aaggcggcgg aactggtggt acgattacag gaagctgggg caatccattc
     1201 ccaggaagta gcttagataa aaattcgttc agtggaggac aactttttgg gaaaaatcca
     1261 ggaggagaat tccgacctaa cgggttccat gatggactag actttggttc agtcgatcat
     1321 ccaggaagtg agattcatgc ggttcatggc ggaaaagtcg tgtacgttgg caatcctggt
     1381 atttcaggtt tgggtgcgtg tgtgatcgtg attaattacg atggcttgaa tatggtgtat
     1441 caagaatttg caaatagtac aggaaattct cgagtgaaag taggcgatca agtgaaagtt
     1501 ggtcaagtaa ttgggatacg agatacagca cacttgcatt taggatttac tcgaatggat
     1561 tggcgtcaag cacaaggaca tgcattcatt gatgatggta catggatcga tccgttacca
     1621 tttttgaata gcagcaaaaa atagatcagg agaaaaaata tggaaaataa aaccttaaaa
     1681 atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atattgcgtt gttttggcag
     1741 cttaaagaag aacaagagac aaatacctat aaaattgaag aagtggctaa aaacgcgtct
     1801 gataagctca aaaaaaattc ttctgaggca caggcgaaga tcgacaagtt aaatcaaacc
     1861 atcaatgaga aaaaccaaga aatcgatcag ctgaagcaac aagatggcac aaaagcaaaa
     1921 gaggaagtaa ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt ggccattgat
     1981 aaatcattaa gcagaaatga attaacggaa aaactaaaag atgtcgcaac ccaagaagtg
     2041 atcgacaaat taagtcctgc agacgatgat caccaacatg acgactacgg aagttactct
     2101 tttacattag gtgacgtgca aacttacgct caaacaagca gtgtaaaaga ggaacaaaat
     2161 tttgtcgtat ttgttgatta tacaatcggc aatcctcaat ttaaagatgt caaaccacaa
     2221 aaaataaaag gtggattaac tgtcactgaa aaacaagtag atggtcaatg gaaagtcaca
     2281 gactttagtt atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag gagcaagaca
     2341 acgttttaaa aaacgaaaaa gttatcttag atggctgggg atctttatga ttctcggctt
     2401 tttgattctt gctagtccga tttacacaca taaaaaggta gacataccag atgaagtaag
     2461 cacgaacgca tttttaaaca cgaaggaact gacgatggtt tcaaaagaaa aaggagcgaa
     2521 ccagcaatta gtttttgaat ttgcgatcga cgatgaagat cagagcttat taaaagaact
     2581 agctaatctt aattatcgtg tagaagtaaa gacagctaaa ggggattatc aagcaataaa
     2641 agccaaagtg atcaaggtgt ctgatgatta ttttgtagtg aagttagcac atgtaccaga
     2701 agaatatatc gccatgcgtt taaccattat tcctgaaaaa attgatccaa aagtggatat
     2761 gcaagaacca caagatctga tttattacgt tcacgaagat aaggttaaag atcaagtgaa
     2821 agatgaagat tatgaacaac atgcgatcaa ttacaaagtg aaaggataca aaaaagaaca
     2881 aaaagcagca caaaaacaaa tggaatcctt ccaagcaacc attgatttga atgaagaatt
     2941 agtgaagaag ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga
     3001 aaacaagatt aatagctacc agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca
     3061 agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta
     3121 gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc
     3181 tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg
     3241 agaagtgaat ttatctaata caacaatatc tgagttgaag aatggtcgaa agaatttaga
     3301 tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat attttacagc
     3361 tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg
     3421 aatcagctat atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt
     3481 ctaacgcaaa catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc
     3541 aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc
     3601 atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata
     3661 cacaaataac cttaattt
//
LOCUS       DASJRQ010000159         3576 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJRQ010000159 DASJRQ010000000
VERSION     DASJRQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.164"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..398
                     /locus_tag="U4A80_002660"
     CDS             <1..398
                     /locus_tag="U4A80_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976252.1"
                     /translation="FLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKE
                     FGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNA
                     ISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            412..1101
                     /locus_tag="U4A80_002661"
     CDS             412..1101
                     /locus_tag="U4A80_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976253.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1226..>3576
                     /locus_tag="U4A80_002662"
     CDS             1226..>3576
                     /locus_tag="U4A80_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1976254.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1212 a          469 c          750 g         1145 t
ORIGIN      
        1 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
       61 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      121 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      181 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      241 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      301 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      361 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      421 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      481 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      541 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      601 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      661 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
      721 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
      781 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
      841 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
      901 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
      961 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1021 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1081 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1141 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1201 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1261 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1321 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1381 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1441 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1501 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1561 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1621 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1681 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     1741 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     1801 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     1861 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     1921 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     1981 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2041 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2101 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2161 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2221 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2281 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2341 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2401 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2461 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2521 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2581 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2641 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     2701 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     2761 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     2821 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     2881 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     2941 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3001 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3061 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3121 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3181 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3241 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3301 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3361 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3421 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3481 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3541 tattcaattt caagaaaata gaagtaaact taatta
//
LOCUS       DASJRQ010000160         3506 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJRQ010000160 DASJRQ010000000
VERSION     DASJRQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.165"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..457
                     /locus_tag="U4A80_002663"
     CDS             <1..457
                     /locus_tag="U4A80_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976255.1"
                     /translation="KEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVF
                     ASAKILDKKKREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKN
                     HIHKSLTYISEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            812..1048
                     /locus_tag="U4A80_002664"
     CDS             812..1048
                     /locus_tag="U4A80_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1976256.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            1045..1458
                     /locus_tag="U4A80_002665"
     CDS             1045..1458
                     /locus_tag="U4A80_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1976257.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            1977..2927
                     /locus_tag="U4A80_002666"
     CDS             1977..2927
                     /locus_tag="U4A80_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEN1976258.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(2924..>3506)
                     /locus_tag="U4A80_002667"
     CDS             complement(2924..>3506)
                     /locus_tag="U4A80_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976259.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1282 a          479 c          627 g         1118 t
ORIGIN      
        1 taaagagttt tttaaagaac agataaataa tagctatgag ttgggttatc attttactgt
       61 ttattatgaa gatattatca agaggttaga agacttggag tggtatgctt attatgattt
      121 attagaggtt tttgcaagtg ctaaaatctt ggataaaaag aaaagagagg aagtaaataa
      181 aattctggag tcggaaaatt ctggttacag attaagcgaa agcaatgaat ttattccaat
      241 cacagatgat atttctatta agtctataaa tgctgtatct gaatcaccat ttaattacgc
      301 aaaaaatcat atccataaat ctcttacata tataagcgag aaggaaaatc aagattataa
      361 tactgtagtg agagaagcaa ttagtgctgt cgaaagttgt cttatcgaat tttcaggatt
      421 accagctaat aaaaaaatac tttgggaact gctataaaag caattaaaga caaatcgaat
      481 aacaatgtcg atcttacatt tttaaaacca tttgagacaa tgtatggttt ggcgagtaat
      541 aatggaatac gacatgcaga aaatgaaaaa acaattgtct cagatttacc tgatgcagtt
      601 ttggtgttaa caacatgttc agccgtaatt aattatttgg gagtaaaaat agcatatgac
      661 tccaataaat catgaaaagt taaaattcat ccaagagcta cattataact attaatttcc
      721 aactctttaa aagtttagta attgaaggaa gtaataaggg tgattgaaca atacggatat
      781 acggtgtata cttatcgtag gagttgatga tatgaatatt aaaaaacgca aaatcagaga
      841 agtaggtaat tcaatagttg taactttgtc aaaagagagt ttattgcaaa aaggtttgaa
      901 acctggagat actattttta ttgatgaaga taaaatgatg gaagctattg taaaagagga
      961 aagcaagata gatcttgaaa ttgatatgta tgttaaccaa gctttatctg agtatgatgc
     1021 agctttcaag gaattagttg aaaaatgatc tatctaagtg aaaaagatat tgtaaaattg
     1081 aatgttattc aaataaagaa atattcaccg cgagaaacaa tcggagtcat tgacaaaaat
     1141 gcgctgagta tggctgtaaa tcaacctaaa cagcatattt ttggaaaaga tctgtatcct
     1201 gataaattta gcaaagcagc tgttctgcta ataaatctga tcaaaaagca tccatttcat
     1261 aatgctaaca aacgtacagc atttttagcc gcgtatgttt ttctcaagct aaatggctat
     1321 tcactaaaaa tgaaaaatca ggaagtagta gaatttgtag ttgaaattgc tacgtatcag
     1381 ggtgattttg atgacttaaa agaggcaaca acagatatac taaaaaagaa ttcaacaaag
     1441 accgattcaa aaaagtagaa gcaaactagg gattcagtta aatctgaaga tttagtttgt
     1501 tctttatttt agtttacata agatacatta tgcgaagttc attattttaa aagcttaaat
     1561 aataaacttt tttataatac caatttattt atgtaaacca atgaccttaa tcgcttaagc
     1621 aagcatgtta tgtaatactc tactctctcc aaaactaatg actatctatt ttcaaagaag
     1681 ctagaatgat tatctgatca aatgataatt tatatttaat tcctggtcta ctgctcttct
     1741 tttaatcaat taatgtctaa cgtactgtac acgataaagt aggaagactt attttcaact
     1801 tcggattcat ttttttaatt cggtgaaaat cactaaatta agagtttatc caagttcatt
     1861 ttagctgaga atacgcttga aaataatttt gatgaatttt cctgaattaa aatttattaa
     1921 cttgttgagt gatataattg taatgtatta ccaaatagtt agcacttaga ttagtgatgg
     1981 aaaggaatag tgagaacaag atgactggta gaaatcccaa agtatttata tcatattcat
     2041 gggagaataa ggaaaataaa gaatgggcta gagaactagc agacaaatta cttgaaaacg
     2101 gtattgatgc atatatagat cagtatgatt taagtttagg tgatagatta cctcagttta
     2161 tggagcagca aatatccgat tcagattatg ttctaattat atgtacacca gagtataaaa
     2221 aaaaggcaaa taatcgtaca agtggtgttg ggtatgaagg tcatattatc tcagcagaat
     2281 tgctttctgg aaaaaatgaa cgtaaattta tcccaataat taaagaagga cgtgcaagcg
     2341 aagtacttcc tacttttctt gctggaaagc ttggaattga tttatcagat agccagcatt
     2401 atgatgaaaa cttagatgat ttaattacaa ctatatttgg tgttaagaga aaaccagcat
     2461 taggtgatat acctaggcat attaaaggca gtcctttact acctatagaa cataatgaaa
     2521 ataaagatat tgaaatctta ggaattatca ctgataaagt aactgtacct aaaatggatg
     2581 gctcaagggg ttctgcatta tataaaattc catttagatt aaatagagta ccaagtcagc
     2641 tttggaaaga gttgtttgtt agaaattgga attcaccacc acgatataca acaatgcata
     2701 gaccaggaat tgcttctgtt gtagacgatg aaattttact taatggaaca actattgagg
     2761 aagtccggga atatcatcgg gaaactttaa aactttgcat tgaaaaaagt aatactcaag
     2821 aaaaaaaaat attgctagag gaggaacaaa aaaaagagag agagaatgag cgagtcagat
     2881 cacataaaga aagtgttcag aaattagcag atgaattaga gttctagttg atagtcagtt
     2941 ctttcttaat tcgatacaca gtttgtctag ttacattata gtctgttgct atttttgaaa
     3001 tagcaacacc attctcctgg tcctgaacga tgcttctata aacaaggcga cgttgaggat
     3061 cttttgtgtt cgcactgtat aacttaggac gtcctttata gacgccgttt gctttagcaa
     3121 tctttatccc ttgtgcctgg cgtcgttttg attcagtacg ctcttgttca gcaatcatgg
     3181 ccaagatttg aatgattagg tctttgataa atttatccag gagaggattt ccgattgctt
     3241 cagccatgat cggtaaatta gtgataataa gtcgcacatt tttcttcttt agataattta
     3301 ctgaatcgat aatctcgtca taattacgtc caagtcgatc gatagcctct acaataaaaa
     3361 tatcttggtc tctaacaaaa tctagaactt cctgaaaaat cggtcgatga gtaatagtcg
     3421 ctccagattg tttctcgatg aaaatttttt cagcaccaaa ttttctcatt tcctcgattt
     3481 gacgttgttc attttgatcg atcgat
//
LOCUS       DASJRQ010000161         3448 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJRQ010000161 DASJRQ010000000
VERSION     DASJRQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.166"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4A80_002668"
     CDS             complement(150..1337)
                     /locus_tag="U4A80_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1976260.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="U4A80_002669"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="U4A80_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="U4A80_002670"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="U4A80_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1976261.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3448
                     /locus_tag="U4A80_002671"
     CDS             3305..>3448
                     /locus_tag="U4A80_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976262.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          977 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttt
//
LOCUS       DASJRQ010000162         3447 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJRQ010000162 DASJRQ010000000
VERSION     DASJRQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.167"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            239..2005
                     /locus_tag="U4A80_002672"
     CDS             239..2005
                     /locus_tag="U4A80_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1976263.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2107..3366
                     /locus_tag="U4A80_002673"
     CDS             2107..3366
                     /locus_tag="U4A80_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1976264.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
BASE COUNT         1232 a          557 c          733 g          925 t
ORIGIN      
        1 aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta
       61 aatagtacag gaagtagatt aaaactttta aaagttttaa gtctatctat ttggcaagtt
      121 taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc
      181 ttttgtttct ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat
      241 ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac
      301 aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctttg
      361 tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa
      421 atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaaggcg
      481 cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca
      541 aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc
      601 ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta
      661 tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt
      721 accttgggga agtaaagatc cagaccgaga agacttcttc gggggagatt tgcaaggtgt
      781 gagggatcat ttggattact tgacggacct tggcgtcaat ggcatctatt tttgtcccat
      841 atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc
      901 ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg
      961 tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt
     1021 tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt
     1081 agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt
     1141 tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt
     1201 gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt
     1261 tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa
     1321 accagacatc tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga
     1381 tgaattccat gcagtgatga attatgcatt cacagattcg attaaagatt attttgcaaa
     1441 aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag
     1501 agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt
     1561 gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca
     1621 aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc
     1681 ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt
     1741 tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac
     1801 tcagtggcta atagtatatg atcgtgaaga taagctctat ttcacacggg aactagaggg
     1861 acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa
     1921 tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca
     1981 atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca
     2041 aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac acaggaggaa
     2101 atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc
     2161 ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa
     2221 ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa
     2281 aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat
     2341 cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca
     2401 gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga
     2461 aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac
     2521 ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca
     2581 gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag
     2641 tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat
     2701 ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat
     2761 atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa
     2821 aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa
     2881 ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa
     2941 gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat
     3001 caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc
     3061 tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg
     3121 gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa
     3181 ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa
     3241 atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa
     3301 acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa
     3361 tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa
     3421 agtggcaaga gcgagatgga aaattat
//
LOCUS       DASJRQ010000163         3429 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJRQ010000163 DASJRQ010000000
VERSION     DASJRQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.168"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..767
                     /gene="erm(B)"
                     /locus_tag="U4A80_002674"
     CDS             30..767
                     /gene="erm(B)"
                     /locus_tag="U4A80_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1976265.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1030..1893)
                     /locus_tag="U4A80_002675"
     CDS             complement(1030..1893)
                     /locus_tag="U4A80_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1976266.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1895..2167)
                     /locus_tag="U4A80_002676"
     CDS             complement(1895..2167)
                     /locus_tag="U4A80_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1976267.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2184..2393)
                     /locus_tag="U4A80_002677"
     CDS             complement(2184..2393)
                     /locus_tag="U4A80_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1976268.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2492..3388)
                     /locus_tag="U4A80_002678"
     CDS             complement(2492..3388)
                     /locus_tag="U4A80_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1976269.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1031 a          619 c          531 g         1248 t
ORIGIN      
        1 atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat tctcaaaact
       61 ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg
      121 ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa
      181 gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat
      241 taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt caattcccta
      301 acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta
      361 aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct
      421 acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga
      481 ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg
      541 tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg aagctatata
      601 cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt
      661 ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag
      721 tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc
      781 ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata
      841 gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa aaaacagcga
      901 aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg accccaacat
      961 gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac tcttttcctt
     1021 ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg taggtagctg
     1081 caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt gttgattttg
     1141 aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg atactttacg
     1201 atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta agcttgaata
     1261 gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg ttttatgaag
     1321 ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt ttggtgttgc
     1381 cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg ttcctaagta
     1441 tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt tagattgaag
     1501 cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa tcactaaatt
     1561 atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg aataaggggt
     1621 aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat ttgggtgctg
     1681 ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt caaaaattgc
     1741 tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag cggttggcga
     1801 ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac gatcttcaaa
     1861 ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct ttattcaaaa
     1921 tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt aattcttcaa
     1981 atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa agattcactt
     2041 ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt aatacccgat
     2101 tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt tcatacgtaa
     2161 ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg tatatattct
     2221 tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt ttcgattttt
     2281 atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat atcttttttt
     2341 gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc cacgatcgct
     2401 cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa atcacaagtg
     2461 atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcattttct aattgaatga
     2521 ttcgctcaat catttcaaaa aatacgttct catacataga caaaactttt ttgtcatagc
     2581 ctttgtgttc tgtaatgcca tttttagacc aagtacttac tttattactt cgcttgataa
     2641 tatttcggaa aaccaaatta tcctctttat gttctttgta cagttcttcc aggtttgatt
     2701 ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accaatcata tctagtccag
     2761 ggttaaactg ttcttgcaaa tcaatcaaat aggaaatata gttttgaatg ttgtttgtac
     2821 tttcttcttc tgcttgtaaa gggatcataa cgtaatcact cgccacgatt gcattatttg
     2881 tataaacgct tggcgttggt acagtatcaa taataataag atcatagtca ctttttaaag
     2941 gtgctaaaag agtagcaagc aatctacttt cattttcaaa cgtccatgag cgagttaatt
     3001 ttggcagtaa catcaaatca aacgtgccag ggatcaagtc taaattatca gtcaaatgaa
     3061 taatagaaga agccaagttt ccatttttca agccttcata aaaattgaca cgtggcaatt
     3121 ctacctcaaa agtttttgct aagtcttttg tcaatgttgc ctgtaagtcc ttatcgatca
     3181 ttaaaacttt taaattcaat ttgtctgtca agtaagcaaa catagtcgat aatttggact
     3241 ttccaacacc acctttaaag taattattca agataataat tgcttcattt ttattgttta
     3301 aaatacgtct taattcttca agtattttta gtttttcctt ctccataact acgccccatt
     3361 ctttttttgt atagtaatat tgtatcatta agaatcacaa atcacaagtg atttgtgatt
     3421 aatcacttg
//
LOCUS       DASJRQ010000164         3379 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJRQ010000164 DASJRQ010000000
VERSION     DASJRQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.169"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..756
                     /locus_tag="U4A80_002679"
     CDS             <1..756
                     /locus_tag="U4A80_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976270.1"
                     /translation="PKIKNKKKTVNYQTVFFLFTTNLAKKIYMALIIVLFLFFTSFFV
                     LNNHSSTSTDSQSYHELLKKKDYEKIIKQYPEKENELVEKLYSEKNSKELKELAEKSD
                     SQIALFYWDFLNEHWQAVTTMKNVTPNETIQAMRGYAYLKQGKLDEAELINKEIDNQT
                     LKDQIYQFKKQEAYKALHEKDIEKAEKINKEINDSELSEDIKVAKSMVNLLQKYEKDR
                     SDSKLSEDERKEAENNYKLWSENLKQLGGNDNA"
     gene            749..2329
                     /locus_tag="U4A80_002680"
     CDS             749..2329
                     /locus_tag="U4A80_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976271.1"
                     /translation="MHNKKKFLVVGLFSAVLLGGAVTYGMYSQQPHNETEKTEQTTKS
                     ESSKHKSKKSAWEESVKGKEDKKEKNKHSTKKADDILAVIADTPLIKQQPNQNKNNLI
                     DRLAVALDDQTKKEADRQEKLIIKDKKSVDEPVLVDLSYKPQLEDTKPSNKENGKTEN
                     NKDENSELDKGNDTKPDNSLPDPLPVPEPNPLPDPTPSPDPNPIPTPDPDPDPEPDPT
                     PDPEPSIDEQINQSKEELNDSRKQADELNKQLTQVKDSLDNLQSIGASTEKNVDKAAL
                     QWQKVADLVNEYNQLTNEIKKLIEEDGTVLTINYDLYKETYEKLNQKVTEVKTAQKEA
                     NNTTNEINKNVEQATDTLNNLEETKKEYQNKQKDVSEVDSNVNSAINHAQSNEQVASA
                     VQDEINQAAQSNEQLNETNKTVGEQLNQVDPIESQTDIASAKEQTVDINNQAEEQNNN
                     VADVMNDFNNLPKPSDSEEPSIPENTDQNQDSTSQPQNIDNASVSQNKIENEQVSEKT
                     LPVTNQNSNQSEIEDIQK"
     gene            2348..3379
                     /locus_tag="U4A80_002681"
     CDS             2348..3379
                     /locus_tag="U4A80_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976272.1"
                     /translation="MSYKDSEAKKTNKTAELLSQAEEGIKEVLSSDKYKNFLRVMSKF
                     HNYSLNNTLLILMQNPNATHVAGYKTWKNSFNRQVNKGEKSIKIIGGRPYKRKVEEIK
                     NGQKEEKEVEGISFFPVSVFDVSQTSGEPLPQLMNELSGEVKNYPELYVALKQSTNFS
                     IEFADIQGGTKGYCSPLEKKIVLNNGMSQAQTIKTLIHEITHADLHAPEFDKNSSIKT
                     TKSTKEVEAESTAFVVCEHYGIDTKDYSFPYLAAWSSDKELKELKNSFEVILKQADNL
                     IQKIDKNLAELQKEVTKEKEEKQSTLSLSDQLEEFEDKAKSLNEQRDKEKLINKIRQP
                     KEQTDVSTL"
BASE COUNT         1492 a          501 c          552 g          834 t
ORIGIN      
        1 ccaaaaataa agaataaaaa gaagaccgta aattatcaga cggtcttctt tttatttact
       61 actaatttag caaaaaaaat ctacatggct ttgataatag ttctgttcct atttttcaca
      121 agtttttttg tattaaataa tcattcatca acttctactg attctcaaag ctatcatgag
      181 ctgttaaaga agaaagatta tgaaaaaata ataaaacaat atcctgaaaa agaaaacgaa
      241 ttagtagaaa aactgtatag cgaaaaaaat tctaaggaat taaaagaatt ggcagagaaa
      301 tctgattcac agatagctct tttttattgg gatttcttga atgagcattg gcaagcagta
      361 actacgatga aaaatgttac accaaacgaa acaatacaag ctatgcgtgg ttatgcatac
      421 ttaaaacaag gaaaactaga cgaagcagaa ctaattaaca aagaaattga taatcaaaca
      481 ttaaaagatc aaatttatca atttaaaaag caagaagcat ataaagctct gcatgaaaaa
      541 gatatcgaga aggcagagaa aataaataaa gaaataaatg attctgaact atccgaagat
      601 attaaagtag ctaaaagtat ggttaatcta cttcaaaaat atgaaaagga cagatcggat
      661 tcaaaattat cagaagatga aagaaaagaa gctgagaaca attacaaatt gtggtcagaa
      721 aacttaaaac aactaggagg aaacgataat gcataacaaa aagaaatttt tagtagtggg
      781 actattttca gctgttttat tagggggtgc agttacttat ggcatgtatt ctcaacaacc
      841 acacaatgag actgaaaaga cagaacaaac aactaaatca gagtccagca aacacaaaag
      901 taagaaatca gcatgggaag aaagtgtgaa aggcaaagaa gacaaaaagg aaaaaaacaa
      961 acattctaca aagaaagctg atgatatatt ggctgttatt gctgatacac cgctaattaa
     1021 acaacaacct aatcaaaaca aaaataatct aattgataga ttagcagtag ctttagatga
     1081 tcagactaaa aaagaggcag acagacaaga aaaattaatt attaaagaca aaaaatctgt
     1141 agatgagcca gtactagttg atttgagtta taaacctcaa cttgaggata ctaaaccttc
     1201 aaataaagag aacggtaaaa cagaaaataa taaagatgaa aatagcgaat tagataaagg
     1261 aaatgatact aaacctgata attcattacc tgatccattg cctgtaccag agccaaatcc
     1321 attgcctgat ccaacaccta gtccagaccc aaatccaata ccaacaccag atccagaccc
     1381 tgatccagag cctgatccaa caccagatcc agaaccttca attgatgaac aaattaatca
     1441 atctaaggaa gaactcaatg attctaggaa acaggctgat gaattaaata aacaactaac
     1501 tcaagtaaaa gatagcttgg ataacttaca aagtattgga gcaagtactg aaaagaacgt
     1561 ggataaagca gcattacaat ggcaaaaagt tgctgactta gtcaatgaat ataatcaact
     1621 tacaaatgag atcaagaaat taattgaaga agacggaaca gtattgacaa tcaattatga
     1681 cctttataaa gaaacgtatg aaaaactaaa ccaaaaagtc actgaagtaa aaactgcaca
     1741 aaaagaagca aataatacga caaacgaaat aaataaaaat gtagaacaag caactgatac
     1801 gctaaataat ttagaagaaa ctaaaaaaga atatcaaaat aaacagaaag atgtttctga
     1861 ggtggattct aacgtcaatt cggctattaa ccatgcacaa tccaacgaac aagttgcttc
     1921 agctgttcaa gatgagataa atcaagctgc acaatctaat gagcaactaa atgaaactaa
     1981 taagactgtt ggagaacagt tgaatcaagt tgatccaatt gaaagccaaa ctgatattgc
     2041 tagcgctaag gagcaaactg ttgatatcaa taatcaagct gaagaacaaa ataataatgt
     2101 tgccgatgtg atgaacgatt ttaacaattt gccaaagccg agtgacagtg aagaacctag
     2161 cattccagaa aatacagatc aaaatcaaga ttcaacaagc caaccacaaa acatagataa
     2221 tgctagtgtt tctcaaaaca aaatagaaaa tgaacaggtt agcgaaaaaa cattaccagt
     2281 tactaatcaa aatagtaacc aatccgaaat tgaagatatt caaaaataaa agtgaggaat
     2341 gaaataaatg tcttataaag atagtgaagc aaaaaaaact aataaaaccg ctgaattact
     2401 ttcacaggca gaagaaggta ttaaagaagt attatcttca gacaaataca aaaattttct
     2461 tcgtgttatg tctaagtttc ataattacag cttaaataat acccttttaa ttcttatgca
     2521 aaatcctaat gctactcatg ttgctggtta caaaacatgg aaaaactctt ttaatagaca
     2581 agtaaataaa ggagaaaaat ccattaaaat aattggtggt cgtccctata agaggaaagt
     2641 agaagaaata aagaatgggc aaaaagaaga aaaagaagtt gaaggtattt catttttccc
     2701 tgtttcagtt tttgatgttt ctcaaacttc aggagaacct ttaccgcaac taatgaatga
     2761 attaagtgga gaagtaaaaa attatccaga gctttatgtt gccttgaagc aaagcaccaa
     2821 tttttctata gaatttgcag atatacaagg tggcacaaaa ggatattgtt ctccattaga
     2881 aaagaaaatt gtgttaaata atggtatgag tcaagcgcaa acaataaaaa ctctgattca
     2941 tgagatcact catgctgatt tacacgcacc agaatttgat aaaaattcgt caataaaaac
     3001 tacaaaatct acaaaagaag ttgaagctga aagtacggct tttgttgttt gtgaacatta
     3061 tggaatagat acaaaagact atagctttcc atatttagca gcttggtcta gtgataaaga
     3121 gttaaaggaa ttgaaaaata gctttgaagt cattttaaag caagcagata atttaattca
     3181 aaaaatagac aagaatttag cagaattaca aaaagaagtg acaaaagaaa aagaagaaaa
     3241 acaaagtact ttgtcactat ctgatcaatt agaagaattt gaagataaag caaaatcttt
     3301 aaatgaacaa cgtgacaaag aaaaattaat taataaaatt cgccagccaa aagagcaaac
     3361 agacgtttct acactatag
//
LOCUS       DASJRQ010000165         3352 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJRQ010000165 DASJRQ010000000
VERSION     DASJRQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.170"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            210..476
                     /locus_tag="U4A80_002682"
     CDS             210..476
                     /locus_tag="U4A80_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1976273.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="U4A80_002683"
     CDS             503..802
                     /locus_tag="U4A80_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEN1976274.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="U4A80_002684"
     CDS             976..1527
                     /locus_tag="U4A80_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEN1976275.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="U4A80_002685"
     CDS             1673..1966
                     /locus_tag="U4A80_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976276.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="U4A80_002686"
     CDS             1959..2255
                     /locus_tag="U4A80_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976277.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="U4A80_002687"
     CDS             2330..3328
                     /locus_tag="U4A80_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1976278.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1261 a          459 c          663 g          969 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat atcaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aa
//
LOCUS       DASJRQ010000166         3328 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJRQ010000166 DASJRQ010000000
VERSION     DASJRQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.171"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..506)
                     /locus_tag="U4A80_002688"
     CDS             complement(<1..506)
                     /locus_tag="U4A80_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976279.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            778..3249
                     /locus_tag="U4A80_002689"
     CDS             778..3249
                     /locus_tag="U4A80_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1976280.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
BASE COUNT         1076 a          601 c          670 g          981 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt ttttgctata
      661 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
      721 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
      781 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
      841 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
      901 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
      961 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     1021 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     1081 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     1141 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     1201 gtaatagatg acattgttat tttagagact ggatatcaaa ttccagcaga tgcagaggtc
     1261 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     1321 aaagaaatag gtgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     1381 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     1441 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     1501 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     1561 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     1621 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     1681 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     1741 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct
     1801 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     1861 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     1921 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     1981 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     2041 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     2101 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     2161 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     2221 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     2281 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     2341 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     2401 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     2461 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     2521 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     2581 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     2641 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     2701 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     2761 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     2821 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     2881 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     2941 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     3001 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     3061 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     3121 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     3181 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     3241 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     3301 cgaagcagga tgagtcatcc aactttaa
//
LOCUS       DASJRQ010000167         3276 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJRQ010000167 DASJRQ010000000
VERSION     DASJRQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.172"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(451..1761)
                     /locus_tag="U4A80_002690"
     CDS             complement(451..1761)
                     /locus_tag="U4A80_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1976281.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="U4A80_002691"
     CDS             complement(1773..3131)
                     /locus_tag="U4A80_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1976282.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT         1027 a          664 c          506 g         1079 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaa
//
LOCUS       DASJRQ010000168         3258 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJRQ010000168 DASJRQ010000000
VERSION     DASJRQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.173"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2177)
                     /locus_tag="U4A80_002692"
     CDS             complement(<1..2177)
                     /locus_tag="U4A80_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976283.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="U4A80_002693"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="U4A80_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          894 a          727 c          526 g         1111 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactat
//
LOCUS       DASJRQ010000169         3256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJRQ010000169 DASJRQ010000000
VERSION     DASJRQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.174"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..692
                     /locus_tag="U4A80_002694"
     CDS             <1..692
                     /locus_tag="U4A80_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1976284.1"
                     /translation="TWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAEN
                     AWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIA
                     GNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQP
                     VVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRD
                     AGAVGGTLYAT"
     gene            682..1422
                     /locus_tag="U4A80_002695"
     CDS             682..1422
                     /locus_tag="U4A80_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1976285.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1419..>3256
                     /locus_tag="U4A80_002696"
     CDS             1419..>3256
                     /locus_tag="U4A80_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1976286.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1223 a          396 c          631 g         1006 t
ORIGIN      
        1 atacttggaa aaatattaaa gatacggcat acaatacgtg gatttctata aaaaatacca
       61 tgattaatac atggaataac attaaggact ctttctggaa tattgttact ggaattgtta
      121 attctgctga aaatgcatgg acaaatctaa aaaatggtgt ttcaaatgct attaatcggg
      181 taaaagaaat ctttaattcg ttaagggaag tcaatttatt tgaaattggc aagaacatca
      241 ttgatggact tataaatggt gtaaaagaaa agtggaatgc tttgaaagat actatcaaag
      301 gtattgcagg taatatcaaa aattggatta aaggagcttt agatattcat tctccatcaa
      361 gatggatgcg tgatatggtt ggtaaaaata ttgttcaagg tattatcgtt ggtattgata
      421 aagagcaaag taagttagat caaaccatga caaatttagt aaaaacaccg tctgttcaac
      481 ctgtagtaaa tggaataagt cacctaccaa ttacacaaac aaagcaaaat atatctgcta
      541 gtgaaacaaa agaaattcat ttacatttaa atgtttatgg agatttacct gattcaatgg
      601 ttcggaagat taccacaaaa attaaaacag aattgacaag acaaatgaaa cgagatgctg
      661 gtgcagtagg aggaacatta tatgcaactt aagagaggac aattttttat taatcaacat
      721 tattcttctg aatttaatgt gtatattcaa aacaggcctg cttctgtttc agctagtcga
      781 gtaattgaat tgagagagcg agaaggaaat gattctatca ttattgataa aggctattac
      841 aaaaatgtga ctagaaagat tgagtgttac tataaagcac cttcaattga tatggtgcag
      901 gagtgggaag atcgaatcac tgaatggtta gatatgtcgt cttatagtga ttttattctt
      961 tactatgatg aacaatatat ttatcaagct gtagtaatag aaggacctga atttaaagga
     1021 acaagaaaaa caggaaatat tgtccctttt gaatttacag tgagtattcg accatttaag
     1081 gaaaattaca atggtagatt tactattcga caaacagaaa cttttgaaat ttataaccca
     1141 gagaagtatt cttcaaaacc gcttattaaa ttgagtggtt ctggagatgc ttctttttat
     1201 attaataagg ataaatatga tttgaaatcg ttggacagag aattatttat agattctaaa
     1261 ttagaagagg cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta
     1321 tttttagatt ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt
     1381 cattcatttg aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc
     1441 ctactgaaac agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg
     1501 gaatatatga agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta
     1561 gatttgatga atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag
     1621 agtatcatat cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta
     1681 tttatgctca gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg
     1741 actctaaaac tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg
     1801 acatacgact ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg
     1861 ttttaaattg tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta
     1921 aacgtgaacc atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga
     1981 ttcggtatgg aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa
     2041 caagaattat tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg
     2101 gttcgccagt agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc
     2161 agtttacaga agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt
     2221 actttaaaac gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg
     2281 ataagttgac tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt
     2341 ttgatacatt taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg
     2401 gactacaata cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag
     2461 ttgcttttta tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga
     2521 ataattatat gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg
     2581 caggaggata tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata
     2641 atccagattt agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt
     2701 ctaagaaagg atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg
     2761 ccaattttat acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga
     2821 ctggagatgt attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa
     2881 taatggagtt aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga
     2941 ttggaacaac tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg
     3001 aagataattc tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta
     3061 aagtaggaaa aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag
     3121 atgtacaagg aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc
     3181 catcgggagg tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa
     3241 agagagcttg gagaat
//
LOCUS       DASJRQ010000170         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJRQ010000170 DASJRQ010000000
VERSION     DASJRQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.175"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4A80_002697"
     CDS             301..585
                     /locus_tag="U4A80_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976287.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4A80_002698"
     CDS             588..1004
                     /locus_tag="U4A80_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976288.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4A80_002699"
     CDS             complement(1141..1860)
                     /locus_tag="U4A80_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1976289.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="U4A80_002700"
     CDS             2535..2630
                     /locus_tag="U4A80_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1976290.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="U4A80_002701"
     CDS             complement(2932..3177)
                     /locus_tag="U4A80_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976291.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DASJRQ010000171         3193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJRQ010000171 DASJRQ010000000
VERSION     DASJRQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.176"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..268
                     /locus_tag="U4A80_002702"
     CDS             <1..268
                     /locus_tag="U4A80_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976292.1"
                     /translation="ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLG
                     CQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(327..2129)
                     /locus_tag="U4A80_002703"
     CDS             complement(327..2129)
                     /locus_tag="U4A80_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEN1976293.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFLGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(2192..3034)
                     /locus_tag="U4A80_002704"
     CDS             complement(2192..3034)
                     /locus_tag="U4A80_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976294.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(3050..>3193)
                     /locus_tag="U4A80_002705"
     CDS             complement(3050..>3193)
                     /locus_tag="U4A80_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1976295.1"
                     /translation="QTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGNEIK
                     IGN"
BASE COUNT          959 a          646 c          441 g         1147 t
ORIGIN      
        1 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
       61 actagaccac agcttacttg gaaaacaaag aaaaacagtt gagactgttt tttcttcctt
      121 agaaaaatta ggttgtcaaa atttcaactc acgttctgtt aaagggctag agagtagatt
      181 tgaaagcata ttactggctt acagtgttct attaagtcga gcacaacgac gttttgaagg
      241 aactttgaga tattctttag gatattaaaa ttaactagca ccacgggtat ctattaagaa
      301 gatcctaggt tatcttagat tatctgctaa tctttgtatt tccagcagta tcctccagca
      361 tgtttttgaa ctccatttgc agcatcccta atactttttg agttaacgcc aacttctttt
      421 acagcagaag ctatagtatc gaattctttt attattgttc catcatcagt catttgaata
      481 atctgtcttc cacgcttctt gctaggctca ctaatcgcat cctctttttt gttcgctttt
      541 agaaaatcag atttttcctt agctatccgc tctgcctctt gtttagcaaa taatagctta
      601 attgccttat ccctacgagc ctctaaagta ccaacatcgt gccgtgtttt acgcataatt
      661 ccttcatcaa gaatgagaat ttcattatcg taatccttaa tttttcgata agctttagca
      721 taagaatcat atagcgcagg agcatcatag ccgttatatc gagctttatc aaaaagtgca
      781 atagcttcat ctactttgcc atttttccta gcagtttctc cacgttccca atatggataa
      841 cctgcatcaa atcctttctc ccaattccct atatttcgta tcttgtttaa tgaaactcga
      901 ttatgcaacc cgttaataat tacatcttct ctagatatca ctttttcaat atttaggtta
      961 acttcagtct catcatatcc acgatttttt gcatcgaata ataactcttt catcgctgat
     1021 tttgaaatag atttcagcat actaaggctt tgctctgctt cttttttagt gtaattacta
     1081 acactcatat attcattcat agaattataa caatcgctat aagcactgaa aatataattt
     1141 gaaagaggcg ttaagtcaaa ggggctacga aaatagactc gcacatttgt tgtaccgcct
     1201 tcacttacag tacaaggttc tataggtaaa tcaattccat ttgtagcatc ttttctaata
     1261 atgatgtaat ttcccttctt agcaaactta aactttaaga atgtatatag acaagttaca
     1321 tcgacataat tattactgtt ttttcgatac ctgatccaac tggtgtcgca tccttttttc
     1381 tcaataatat cttgaataaa agcacaagct tcaagttctt ttatgtctgg agtcatcttt
     1441 tctatttgct ttttttgttc ttccaacgac tcctcaataa tatctaaaac acccaaaaaa
     1501 atctttccgc atatatctgc atctgattcc gctctatggg cagtgtcatt tattaatccg
     1561 aaatattccg ctacagtctc ttgcttatga tttacaatgc cttttatatg ttttctagag
     1621 agactcagag tatcaacata cttaatttta gcatcatacc caagtctaga aaatgtgtta
     1681 cttaaaaatc caaaatcgaa acttgcattg tgtgcacaca tagtgatttt tccatctagt
     1741 gcatctccta aaaattctac caactggggg taaacttcct gctcatttgg agcagagttt
     1801 agcatttcat tagttatatt atttattgcc gaagctgagg ctgcaattct aacttttgga
     1861 ttgacaagag ttccgaatga ttgaattgga actccttctt taaatagcac cgcaccaatc
     1921 tctacaattc tatctgaaga agaatttagg ccagttgttt ccacatcaaa agcgataaaa
     1981 gttttacgaa actcatctac atttgatatg tccacttcca atttttcagc aaaaacttta
     2041 ccagtacgaa atagactaga agccagttga taatcaacgt gtttactaac ctttccttgt
     2101 tttttcttta gaaaatcaaa taatcccact attatttcct cccccattaa ttttatccat
     2161 aatcactttt acgatgtctt tttcttatat tctacctatt cgcataccac aagaagtgat
     2221 ctaactttct tcttctaaaa ggaatatgta tattattttc atctagtagt tttccaacac
     2281 attcgttaaa tgcctcgtaa tcaaaacttc ttcgccacgt atcaatctta ttcgtagtta
     2341 gtccttggat atagtgtgga agagtatttt taacgatacc atcaaagatg gagtaatcat
     2401 ctctcccaca cacttccaca ttatgatatg tacagtactt ggatgcaaat gagaacatat
     2461 taactgcacc aatgttcctt gcgataatat ttatcaactg aggatctcct tttgccaacc
     2521 gatcatcaaa atttggaatc tccagaatta ctttagccaa atcatataaa gacaacctgc
     2581 tcttatattg agaaagatgt gttgaatttg ttacatcaat taccgcaatc ttcatcgcaa
     2641 tagtatttaa atcattatga agaggaaatg cattcaaaac atcatgtatt attagacctt
     2701 ctttcccata attagagctt gcacctacaa acttatgctg tttctcaata ttttcttcag
     2761 ttaaagataa gtatttttgt ttatggattg tttcacttat agttttttct ttacttgaat
     2821 caagcttaag aaccttgtca atgcaccaat atgttgttgc tccttttatt ggggaaatat
     2881 cattctgctt aagatattct tttaataaac tcagttgttt tccttctgga atagtaccag
     2941 atttcgtttc tatgatatga ggttgtggtt tggaagtacc ttcattagca gcaaatacag
     3001 catactcaac atcatttatt gttatgattt tcaatacaaa ttacacctcc taattaccta
     3061 tctttatttc attgccatca ggcaatataa aagcctgcac aaacttgaca cctagcacat
     3121 cagcaatcgc tttcaactca tctaaggtta ccgtttctcg ttgtaatttc ttattgaaat
     3181 tctgtggagt ctg
//
LOCUS       DASJRQ010000172         3142 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJRQ010000172 DASJRQ010000000
VERSION     DASJRQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.177"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..434
                     /locus_tag="U4A80_002706"
     CDS             126..434
                     /locus_tag="U4A80_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976296.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            436..858
                     /locus_tag="U4A80_002707"
     CDS             436..858
                     /locus_tag="U4A80_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976297.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            878..2467
                     /locus_tag="U4A80_002708"
     CDS             878..2467
                     /locus_tag="U4A80_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976298.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            2479..2814
                     /locus_tag="U4A80_002709"
     CDS             2479..2814
                     /locus_tag="U4A80_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976299.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2955..>3142
                     /locus_tag="U4A80_002710"
     CDS             2955..>3142
                     /locus_tag="U4A80_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976300.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1255 a          450 c          639 g          798 t
ORIGIN      
        1 tgtgggaaca aaatatttaa aaataggata gtgaaaggat agcaaaggat agtaaaagga
       61 tagaagcagg atagcgaaaa ggataggctc cttttcgcta tcctttttaa attggaggaa
      121 ttaatatgga agtaagaata agaaatgtag atgagaaaat cgtaagagaa attgatcgaa
      181 gatgtgacga actaagtaac cgaacgggta ataaatggag tcgtaacgat tatttaaagc
      241 tactcgttga aaatgatttt gatcgtcctt tgatggaata taaacaagat aaatttgatc
      301 aattattaaa tcagttaata gataaatttt cttttgttca aagtcataat acaaaagtat
      361 tagaagaata tatttgtcag aataatcaaa ttatcgaact tttaattgaa caaagtagag
      421 gaggagaagt ttaacatggc caaagttata gacaagcata tttattttta tgaaacacat
      481 atagatgttc tagatgaaat tattgcaact catgcagagg ttaaaaacta tagtgaagcc
      541 gttcggtttc tatgtatgaa ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa
      601 gcaaagttga atgcaatggg aaaagaaatt tctattttaa ccgaaatggt ttcagagttt
      661 gcggatctcc atttgaaaga tacagggatt ttaattggta caaattcaac tgtttaccaa
      721 gaagcaaaag agcgtgtgga aggcaagata aaagcaaatc aaacgaagaa atattctcct
      781 aagataaaga aaacaataag cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca
      841 aggttcttaa aacgataaaa attagaagga gaatgtgatg agtaaatcac ctgcagtcat
      901 tctcacttgt caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat
      961 gacaagagaa aaagcgctgg aagaaaagga acgacgtacg cccgaggaag aacaggaact
     1021 gcgtctaatc aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga
     1081 gagtaagacg ggtaaagaat tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca
     1141 tggaatcaat tttgacaagt tagaagaaca agattttacc aaatatcttt cttatatgac
     1201 acgtcactat gcgttagaac aaaagaaagt actgacgcat agtgaacaaa aagaatacca
     1261 acgcttgaac caagcaataa aaaaatatca aacagagcca cacaaaaaag cttcaggaaa
     1321 tcttcctgga gttttttcta tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat
     1381 caagcaaacc ttccaaagag gacaacaaaa aggctcaatc atttatcaag atgttgtctc
     1441 tcatgatcat tcgtatcttg aaaaaatagg attgtacgat cccaaaacag atactttaga
     1501 tgaagaaggt ttaaaagcag ctggaagaaa aatgatggag acattgttcg agaaagagca
     1561 actgaatgaa acaggctact ggatggcaac gattcaccga aataccaaac atatccatat
     1621 tcattttgca gttgttgaaa aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg
     1681 tgtgagttat atcgtaccga aagggaagag aaggcaagca acattagacg aaatgaagac
     1741 taattatgta catgagttag aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa
     1801 acgaaattta ttgacacgga aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga
     1861 gccagtacgt tatgatcgac acgcaatgag cttattaact gaagtatata aaaacttacc
     1921 taaaaagcgt tcggaatggc attacgggga tgaaaatcga accaaacttt caccatacac
     1981 aagagagaaa ctcgatgagt taacccaata tgtgttaaca aaagaacctg gatatcaaga
     2041 atatatacag atttcaaaag cattgaaaga agaagcaaaa agtctttatg gggaaagcaa
     2101 gagagaatca aaggatgcag aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa
     2161 tgccattcta acagaactga agaaacagga tagtcaaata aaacctttaa tagaaaggat
     2221 ggatcagatg caatctattt acaatgaacg atcctttcga acacttaaac agttgccaac
     2281 gaatgaatac aaatatagtt acgaagaatt catgaagcgc aggaaacaat tccaacaaag
     2341 agttgtcacg caacggagtt tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa
     2401 ataccacgca agtaaagcat acgaagagca acaacgaaga attcaaatgg aacaagaaag
     2461 acagtaggag gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca
     2521 ttaagagaag aacgaatcga acaacttccc gattgtttag aaaaagagca tttgtttcag
     2581 ataagagaaa tgttgataaa cgaacagttt gatccaaatc aatttttagt cgttgagtac
     2641 ccagctactg gtgtgtactg ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa
     2701 gaatatgagg gcaagttagc gccttattat actacttggg aaatgaatga agaggggatc
     2761 aataacttcc cgtgtaaaag cattgaagag tctatttcac tcacagaatg ttagttgtta
     2821 agtgaataaa agaagtaaaa gagtcctgaa cgtgattgtc caggactctt ttttttcact
     2881 gaattgtttt ccgccaattc attgagtggt gcgatttccg tctacattat atcaaaagaa
     2941 aggaggaaga agaaatggct tattcaacga ttggagttgt tgttgaaaaa agcagagaca
     3001 atcttgtttt tgtgacagaa attcaaacag gcagagcttt tgtagtcaca gacaaagcgg
     3061 caaaagcata tcaaagtggc gatattttaa cgttaaatct gactacgaag acgttcgtcg
     3121 atgcggcaga agattatcca tt
//
LOCUS       DASJRQ010000173         3077 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJRQ010000173 DASJRQ010000000
VERSION     DASJRQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.178"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            469..1641
                     /locus_tag="U4A80_002711"
     CDS             469..1641
                     /locus_tag="U4A80_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HEN1976301.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1771..>3077)
                     /locus_tag="U4A80_002712"
                     /note="aph(2'')-Ia"
     CDS             complement(1771..>3077)
                     /locus_tag="U4A80_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEN1976302.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT         1054 a          553 c          399 g         1071 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tcacttcctt tattattttt cattttttta aactatttaa tactaatgtc
     1741 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
     1801 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
     1861 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
     1921 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
     1981 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
     2041 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
     2101 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
     2161 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
     2221 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
     2281 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
     2341 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
     2401 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
     2461 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttattagt
     2521 actaaatttt gttttaaaaa tgtattcatt attaactaaa tatgccacac tatcataacc
     2581 actaccgatt atttcaatac tatctacttt gaaattatca aagtaatgct caattaaata
     2641 tttcattgcc ttaacatttg tggcattatc atcatatcta tattccatta aataacaatc
     2701 ttcttttttg ccctcgtgta attcatgttc tggcaaatct tcaataattc taaaaccaga
     2761 tttttggtat gcccttattg ctcttggatt atttttatga gggtctaaaa taactgcatt
     2821 agcatttctt tcttttttca aaaattcaaa aatcaattta atatatcttg taccaattcc
     2881 tttactccaa taatttggct ctcctataaa ttgatccata ccatagacta tctcatcagt
     2941 ttttggataa tgataatcag tatataactc atcatacatt ttatatattt gtccatatcc
     3001 aataggaaca ttgttatatt caataattac tctaaaaact tcatcttccc aaggctctgt
     3061 ataatgtttt tttaatg
//
LOCUS       DASJRQ010000174         3038 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJRQ010000174 DASJRQ010000000
VERSION     DASJRQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.179"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="U4A80_002713"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="U4A80_002713"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1976303.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="U4A80_002714"
     CDS             complement(1034..1954)
                     /locus_tag="U4A80_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1976304.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="U4A80_002715"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="U4A80_002715"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1976305.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DASJRQ010000175         2912 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJRQ010000175 DASJRQ010000000
VERSION     DASJRQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.180"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..835
                     /locus_tag="U4A80_002716"
     CDS             119..835
                     /locus_tag="U4A80_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1976306.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            854..2437
                     /locus_tag="U4A80_002717"
     CDS             854..2437
                     /locus_tag="U4A80_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEN1976307.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2572..>2912
                     /locus_tag="U4A80_002718"
     CDS             2572..>2912
                     /locus_tag="U4A80_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976308.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYW"
BASE COUNT         1117 a          316 c          558 g          921 t
ORIGIN      
        1 taaatatcag ttatattaag cagttgtgtt atagaggaag aaacattatt tcaatgatta
       61 agagtcgaga gcacaaacgg tagacgatgt tatttttata aaaagatata atttattaat
      121 ggtcgataat tatgaaagga aaaatattct taaaaaaaag agtataggat gtcgtatgaa
      181 aatagtcgct atagtaccaa tgaagttaaa taacaaaaga ttgccacaaa aaaacacgaa
      241 agcttttacc aatggaaaac cattgtgtta ttatatactt tcaacacttt taaaagtaaa
      301 acagattgat aaagtgtatg tctattgtag taacccagat attaaagaat ttataccaga
      361 aggtgtaaca tttttaaaga gatctagttc cttagatcag gatacaacga gtattacaca
      421 ggtattagcc ggtttcacaa aagaagtaga ggctgatgta tatgtaatga cacacacaac
      481 agcaccattt attagcgaaa aatctattga aaaaggacta gatgccgtgc tgacaggaga
      541 atatgattct gcatttgctg caaaaaaatt gcaagatttc ttatggaaag acggtgaacc
      601 atttaattat gaattggaca atattcctag aacacaggat ttgccgcctt tatacgaaga
      661 aactagtgga ttttatattt ataatcgcaa tgtgatgacc gagttgcata gaagaattgg
      721 gaaaaaacct tgcatagttg aagtaggtga gattgagtca gttgatattg atgaagccga
      781 ggattttatg attgctgatg cgatttataa tcatcttatt ttacaaggta gttaagacag
      841 ggggaaggtt aggatgaaca atgtacagat tttagattgc acacttagag atgggggcta
      901 ttgtaacaat tgggaatttg gatttgataa tacaaaaaaa attatttctg gacttataga
      961 atcaggaatt gagattatag aatgtgggtt tctcacaaat aaaacagtat acaataaaaa
     1021 tgttacaaaa tatacgacct tagaagaatt agcacagata attcctgaaa aaagagaagg
     1081 aaaattattt gtagcaatga tgaactatgg cgaatataat atagaggatc ttccagatta
     1141 tgatggttca tctattgatg gaattaggat tgcgtttcat aaaaaaaatt gcaatgaagc
     1201 gttgaaatta tgtcgtgaga taaagaaaaa aggctactta gtttttgttc aagctatggt
     1261 atctctcaat tatacagatg aagagttttt agcagtaata aaaaatgtga atcagtttaa
     1321 accatacgct ttttatattg ttgatagttt tggaatgatg aaaggaaaag atttaactcg
     1381 tcttttttac atgattgagc ataatctaga aaaagatata cagataggtt ttcactcaca
     1441 taataacatg cagcttgcat attctaatgc acaaaaactt ataaccgtac aaacaaatcg
     1501 aagcctgatt attgattctt cggtgtacgg aatgggaaga ggagcaggta atcttaacac
     1561 agaactattt gtagaatatt tgaatgataa tgcaggaaaa aaatatcaat taaaaccatt
     1621 attatatata attgatgaaa tactaaatag tttctatcag aagagttatt ggggatattc
     1681 attaccaaac tatatttcag cagttcataa tgctcatcct aattatgcaa gctatctgtc
     1741 atataagaat actttaacaa ttgaagcaat gaatgaaata ttcgaaatga tggatgaaga
     1801 aaaaaaggta tcttatgata aacaatatat agaagattta tactacaaat atatggaaac
     1861 tgggaaaata caggaggaat atatggaaga actgagaaaa tttctctcag acaaaaaaat
     1921 tctactgatt gcaccaggaa aaagttctgt agatgaggta gaaagaataa aagagtttgt
     1981 taccaaagaa gaagtcgtaa taattagtgt aaattttgaa tattctcata tagatataga
     2041 ttacgtattt ttaagtaatt taagacgttt tcatgaatta ccgaaagaaa gcagaactaa
     2101 atgtattgtt acatcaaata tacaagcatc ggatgtatat ttacggacga gttataaaaa
     2161 gttgttatct gaaagggaag ttgtaagaga caatgcagga ttaatggcaa ttagattttt
     2221 agccgatatg aatgtggatg aaatattctt agctggattt gatggatatt cacataatga
     2281 agatgagaat tatggtgagc aatccatgga aattataaca aaatttgcat tattagatgc
     2341 aatgaacgtg caaatgagag atgaattgtc agaattagct aaaaaagtaa aggtgacatt
     2401 tttaacggat ccacgttggg ttcgaattga taagtagtag taataatgtt tttaaaattt
     2461 tgataaagag atagtgagag aataaaaatg ggtaaagtct taaataaaaa agaagaaaaa
     2521 tatttaaggg ccgatggggc gagatgaaac tctaattttg aacttttaag aatcatttct
     2581 atattgtgta ttgttgctta ccatgctaat ttgcgtttaa attataatgc agacggaatt
     2641 atagtacatg gttcattaac tgctaaccag attttcttga attatattgg tatttggggt
     2701 cagctaggag ttaattgttt tatatttatt tcggcatatt ttttaagtga gaaaagaaac
     2761 attcactttg aaaatgtttt gggaattgta ttagaatgta ttttttatag tgtattagtt
     2821 tatggaattc tttgtgcatg tggaatagaa acattttcat tgaacagctt tgtaaaagaa
     2881 atattatcat ttgctctagg tagatattgg tt
//
LOCUS       DASJRQ010000176         2876 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJRQ010000176 DASJRQ010000000
VERSION     DASJRQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.181"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..938
                     /locus_tag="U4A80_002719"
     CDS             123..938
                     /locus_tag="U4A80_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976309.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1064..1321
                     /locus_tag="U4A80_002720"
     CDS             1064..1321
                     /locus_tag="U4A80_002720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976310.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1573..2145)
                     /locus_tag="U4A80_002721"
     CDS             complement(1573..2145)
                     /locus_tag="U4A80_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976311.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2161..2766)
                     /locus_tag="U4A80_002722"
     CDS             complement(2161..2766)
                     /locus_tag="U4A80_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN1976312.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          944 a          522 c          446 g          964 t
ORIGIN      
        1 ttaaataaaa aataattact atctagtacg aaattaatta cataggataa tatattaagg
       61 tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa ggagcaataa
      121 gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa ctttataaac
      181 aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac tttattctac
      241 caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat ctgtattact
      301 gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc atagcagagt
      361 ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt gatttaggta
      421 tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt cctctctcta
      481 gttactataa attagccctt gatagttctt taaaggatgt taccgagagc actgtccatg
      541 aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa aatgaggctg
      601 atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga aaaacacaaa
      661 aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat ttcgaattaa
      721 gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa gatctagaag
      781 ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt gtaaacacta
      841 gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata gagcttacta
      901 aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc catcattaag
      961 tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg cttactattt
     1021 attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta atttatatat
     1081 agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg taaaatcaat
     1141 aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga tttttatgat
     1201 agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg atatggagat
     1261 aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc cacgttccta
     1321 aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg ttcattataa
     1381 atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt actttacctt
     1441 ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa tattatattt
     1501 cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt ctggttcgct
     1561 gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg tactgcgact
     1621 gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca gttcatatgc
     1681 gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat taggattatt
     1741 ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac gttccatttc
     1801 tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt caatcgttcc
     1861 tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga tctctaatgc
     1921 ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat ctccaggctt
     1981 cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg taaatttttc
     2041 tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt gttcttttgt
     2101 actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt ccacctactt
     2161 ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga catcgattcc
     2221 tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa ttagatagcg
     2281 tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg ctttatcgat
     2341 taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca gccaaattct
     2401 agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca catatttctt
     2461 gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg aatgttctaa
     2521 atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc
     2581 ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac ctttaaatgt
     2641 accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt tttgtttact
     2701 gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt tattctctaa
     2761 aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct caaccccatt
     2821 atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg atagat
//
LOCUS       DASJRQ010000177         2858 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJRQ010000177 DASJRQ010000000
VERSION     DASJRQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.182"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..226
                     /locus_tag="U4A80_002723"
     CDS             <1..226
                     /locus_tag="U4A80_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEN1976313.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            226..573
                     /locus_tag="U4A80_002724"
     CDS             226..573
                     /locus_tag="U4A80_002724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976314.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            570..1619
                     /locus_tag="U4A80_002725"
     CDS             570..1619
                     /locus_tag="U4A80_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1976315.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1634..1840
                     /locus_tag="U4A80_002726"
     CDS             1634..1840
                     /locus_tag="U4A80_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976316.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1861..2457
                     /locus_tag="U4A80_002727"
     CDS             1861..2457
                     /locus_tag="U4A80_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HEN1976317.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1032 a          395 c          567 g          864 t
ORIGIN      
        1 taaaacaact gaatttgata ttctacacgc tctaatgtcc acacctaata aagtcttttc
       61 tcgtagtgat ttgatagaga aagttaaggg aatagaattt gatggactag ataggagcat
      121 cgattctcat ataaaaaact taagacataa aattgagaat gatgctaaat cacctatgta
      181 tattttgaca gtccatggta caggatacag atttggtggc cactaatgag aaaaagaacg
      241 ataaaaactc aattagctgt ttccttttta gcaatcgcaa cattaataat aggatcgatt
      301 agtttagtcg cattaagttt gatgaacaat cattttagta aatacgtgga agagcggcaa
      361 gaagatttgt tgaatcagta tgtatataca attgatttat tgtggttaaa tagtggggaa
      421 acatggaata gtgaagaact tgcagcacta tctgagaaag tattagagaa taatatttat
      481 ttttctatag aagacgaaca aggcaacatg gtttgggaac ttactggaaa agatttgaag
      541 tcagcacagg agaaactgaa aaaaatgcat tgaaagtttc tgagaaaaac agtgtgaaac
      601 tagacgaaac aatagaagtg aaaaagaagt taattaatga tggaaacgag tttggaaaag
      661 taacgtttta ttatttcgga ccttttgctt atacagagca cgatgccttg tttatctcca
      721 gtatgaaaca aagtttaatg tatgttgcaa ttgcagctct tcttgtatca ttcatacttg
      781 cttcgtggat ctctgctaga ttaggattgc cactaaagca tgtaagcgat tttacacata
      841 aattaactcg tggggaatat gctgataaaa tcccacaaga aacttcaatt attgaaataa
      901 attccttaat tgattcccta aatgatttaa gtaatcagtt ggagaaacag catgggcttc
      961 gaaaaagatt aactaccgat atttcacatg aattaagaac tccattagct acgcttaaag
     1021 gcaatgtgga aggaatgatt gacggagtct ggaaaataac tcccgagcgc ctacaatctt
     1081 gttatgacga aatagatcgt ctaactcgtt taattggaaa tatagaaata atcaacaaaa
     1141 ttgaagcaaa atatgatcat ttaaataaaa ctgagtttaa catatataag ttgattgaat
     1201 ccgttattga aaatttcgcc agcaaaattg agtcaaaaaa ccttcatgtt gaaatccaag
     1261 gggataacat caatatatct gcagataaag ataaaatgaa ccaagttgtt atcaatcttt
     1321 tgaataatgc tattaaattt acgcaaaagg agggaaccat taagttttct ataagtaaaa
     1381 ataaagacca tgttctttta attgttgaag acaacggcat cggtatagaa aaagatcagc
     1441 aacttcatat ttttgataga ttttatatgg ctgacccctc cagaagtaga gctttagggg
     1501 ggcaaggtat cggattagct attgtaaaga gcgttattga agcgcataaa ggtagtataa
     1561 ctgtaaaaag taaattaggt ctaggaacta aatttgttat aaagttacca tttcaataaa
     1621 ggaggagagt tatttgtttc aaaaacagat aaaaattgct aaactagtat gtgaatgttg
     1681 ggcaaaaaaa atattttttc aactgatttc gatagatagt gtcgaaaaag ttcatttaga
     1741 tatcttatca aattctgtag taatcttttc taagaaggac ttatcaatag ctacaattaa
     1801 aaaagaagtt tttggattac aaatttatga gatgacctaa atgtgattaa ttaaaggagg
     1861 atgaagacag tgaaaaagat gacaaaatta gtatttgcaa gtcttggaat tattgtattt
     1921 ttaggagcgt gtagcaatca aagtgaatcg aataattcta atagcacaaa cgaggaatcg
     1981 acttctacag cgtctagtga aatgaatagt atggagggaa tgaatcacga aggtatggtt
     2041 ccttcttcaa tgaaagatgc tgcgaaccct aaattcccgg tgggaagtaa tgttatactg
     2101 ctaggcgatc atatggaagg gatgaaaggt gcaaatgctc aagttgtagg ggcctttgat
     2161 actacaatgt atgaagtaag ctatgagcca aaaactgggg gaccaatggt taacaatcat
     2221 cgatgggtag tacaagaaga gttaaaagat actgagactg tagcaaatga gggcgatact
     2281 gttatcttga atgccgatca catggatggg atgatgggag ctgaagcaaa agtagataaa
     2341 tctattacag gaactgttta cgttgttaat tacactccaa ccgatggtca agaagaagtt
     2401 aagaatcata tgtgggtaac tgaagatgag atggaatatg acgaaaataa tgagtaattt
     2461 tatatactaa accaacaagc tggtacatgg tcttcgtatt ctcaacatga ggaatatgag
     2521 ctctttgtta ataaggactg atgagttgtg caaaatcaaa tctgggtcag aacgaacctc
     2581 attctggctc agatttttgg ctcttcgaca tttagtgctg gtagagagac aactgaaaat
     2641 ttagtcataa ccagttaggc agagtgctta actggttttt gttcgcattt attattcttt
     2701 tgatattggc tgcttgccct tcaattttat ttggtttctt aatcaactct tcatataacc
     2761 ccaaatcata tattaatgag gtgcctccta ttcctataaa tctatctctt ctttaatagt
     2821 atgagaaaag tctactcttg tagatttaaa ttgtcaac
//
LOCUS       DASJRQ010000178         2786 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJRQ010000178 DASJRQ010000000
VERSION     DASJRQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.184"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..237
                     /locus_tag="U4A80_002728"
     CDS             7..237
                     /locus_tag="U4A80_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1976318.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(328..693)
                     /locus_tag="U4A80_002729"
     CDS             complement(328..693)
                     /locus_tag="U4A80_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976319.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            901..1383
                     /locus_tag="U4A80_002730"
     CDS             901..1383
                     /locus_tag="U4A80_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976320.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1580..2470
                     /locus_tag="U4A80_002731"
     CDS             1580..2470
                     /locus_tag="U4A80_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN1976321.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          950 a          447 c          543 g          846 t
ORIGIN      
        1 tcaagtatag gttttagaac taaacgaagt ttgtcttcta aaaaaaattg ggtatactca
       61 caaactattt tttatggagg aattgtttta attagtttac tttcttcaac actttattct
      121 ttgaatatta tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct
      181 gcaattatta cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa
      241 aactagtgat agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc
      301 ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc
      361 taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat
      421 cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa
      481 taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt
      541 cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac
      601 ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag
      661 caacggaagg tttttccagc aagtttttat catctttcat caccacctaa caagtgttat
      721 tattttgtta gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt
      781 ctagattaaa tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa
      841 tttttttaga tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta
      901 atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt
      961 ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc
     1021 caaggaagcg gatttgtggt ttctaggaat tttccagctt tgattgtttt ctatttagtg
     1081 ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt
     1141 tacttttctt gcttattagt atttacatct acgttttctt tgctgcctcg cttgattatc
     1201 ggccctaatg gtttgagcga gagtggattg aaggatttag cttattcctc catgtatttc
     1261 ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt
     1321 tacttctgga cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat
     1381 tgatggtgga tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt ttctcatatg
     1441 ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa
     1501 ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa
     1561 ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct
     1621 tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc
     1681 aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt
     1741 agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca
     1801 aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa
     1861 ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa
     1921 agtagcagtg gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac
     1981 caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg
     2041 gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt
     2101 cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg
     2161 aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata
     2221 tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt
     2281 agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc
     2341 ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt
     2401 caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa
     2461 tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac
     2521 tatgtttccc cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc
     2581 cgttttttga gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag
     2641 ctacgacata gaactgtcgt aacttttttt ggctcttcgt caaatcgtgt tggtgaagac
     2701 aaatttgaat aaaacttaga ccgatatgcg gcgcgttctg tgcgccgcat atttttttag
     2761 ttttcaaaga tcagaccttg ttgtaa
//
LOCUS       DASJRQ010000179         2779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJRQ010000179 DASJRQ010000000
VERSION     DASJRQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.185"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="U4A80_002732"
     CDS             234..1559
                     /locus_tag="U4A80_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1976322.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="U4A80_002733"
     CDS             1552..1902
                     /locus_tag="U4A80_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976323.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="U4A80_002734"
     CDS             1899..2069
                     /locus_tag="U4A80_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976324.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          976 a          440 c          538 g          825 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgta
//
LOCUS       DASJRQ010000180         2778 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJRQ010000180 DASJRQ010000000
VERSION     DASJRQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.186"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..876
                     /locus_tag="U4A80_002735"
     CDS             <1..876
                     /locus_tag="U4A80_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1976325.1"
                     /translation="LTIFFVHRHEDVLRVAKKISGPIVRVKDFTREIAKGDYTSLSPE
                     KTDIKELDELISSVNALSVQLENQQDIRNQLSSDIAHEIRTPLTTLKGSLEAMIDGIW
                     EIIDDRIQSCYDEVNRITRLIGSIDKINEIESHQDSLNKISFDLYALAENISSNFEAL
                     FAKKNIYYALNGDSLFITADRDKISQVITNLLSNAVKFTPPEGTITLKIRNEKNQALL
                     TIADTGEGIHPKEINRIFERFYMSDLSRNSFLGGQGIGLSIVKSIIKAHNGTITVKSD
                     YGKGSTFTVILPVKR"
     gene            complement(1046..2254)
                     /locus_tag="U4A80_002736"
     CDS             complement(1046..2254)
                     /locus_tag="U4A80_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEN1976326.1"
                     /translation="MKKRITLALLSSVGLLLAACGQEESTSSQNSSQQAIVSTSQETS
                     ESQSSSKEENKEQTEAYYTSDTGSVTKVDAQTHEVVDTIAIDGSAHNVQISPDEKVIG
                     VTIVPSEGHAEDSSGSNESMEMEHDEDSDTSMSNESMEMGQDEDSDTSMSNESDDEKG
                     IAAFYDAETGEKLAEVEVGSHPAHIVFTNDGNYAAVSNNGENTVSVIDVSTYEVVETI
                     PTGEGPHGFRIAEDGKTAYVANMGEDTVSVLDLESMEEVNRITVGSTPATTGITSDGQ
                     TLLVTLNGENALAIVDLTTNKVEKVAVGNGPIQLFVQHDDQYVFVANEGTEENPSNTV
                     SKVDLNSNEVVETIEVGSGAHGVVISEDDKYTFVTNAFDDTVSVIENETSEVIVTVEV
                     GETPNGITLK"
     gene            complement(2705..>2778)
                     /locus_tag="U4A80_002737"
     CDS             complement(2705..>2778)
                     /locus_tag="U4A80_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1976327.1"
                     /translation="RRNGTLFGFSVSTEIKVLMGITA"
BASE COUNT          827 a          541 c          444 g          966 t
ORIGIN      
        1 ttaacaattt tttttgtgca tcgacatgaa gatgtactcc gggttgctaa aaagataagt
       61 ggacctatag tgagagtaaa ggattttaca agagaaattg ctaaaggaga ttacacaagt
      121 ttatctcctg aaaaaactga tatcaaggaa ctagatgaac tcatttcatc tgtaaatgct
      181 ctttctgttc aacttgagaa tcaacaagat atcagaaacc aattatcttc tgatatcgct
      241 catgagatca gaactccatt aacaacacta aaaggaagtc tggaagctat gatagatgga
      301 atatgggaaa taatagatga ccgaattcaa agttgttatg acgaggtgaa ccgtattacc
      361 cgccttatag gaagcattga taaaattaat gaaattgaaa gtcatcagga tagtttaaac
      421 aaaatatctt tcgatttata tgctctcgcg gaaaatatat cttctaattt tgaagctctt
      481 tttgctaaga aaaatattta ttatgcatta aatggggatt ctctattcat taccgctgat
      541 agggacaaaa taagtcaagt cattacaaat cttttatcca atgctgttaa atttacaccc
      601 ccagaaggaa caataactct aaaaattcgc aacgaaaaaa atcaagcttt attgactatc
      661 gcagatacgg gagaaggaat tcacccaaaa gaaattaatc gtatttttga gaggttttat
      721 atgtctgatc tttcaagaaa tagttttcta ggcgggcaag gaattggctt atctatcgta
      781 aaaagtatca taaaggctca taacggaacc ataacggtca aaagtgatta tggaaaaggt
      841 tctacattta ccgttattct tcctgtcaaa agatagtaat catcaaaaaa agcggttcac
      901 ttttatgtaa agtgaaccgc tttttcagct tcatgataaa gttttccatt taatttagga
      961 ctaattaaga tttttaaacg ttagaatatt tatccctttt cagaaatcat taagttgatt
     1021 ttttagttta acttaacact aataatcatt tcagagtaat cccatttggt gtttcaccaa
     1081 cctcaaccgt gacaataact tcactagtct cattctcaat aacactgacc gtatcatcga
     1141 atgcattcgt gacaaaggtg tatttatcgt cttcactaat gacaactcca tgtgcaccgc
     1201 tacccacttc gattgtttcg accacttcat tactatttaa atcaaccttt gaaacggtat
     1261 tggaaggatt ctcttcggta ccttcatttg ccacaaatac atattgatca tcatgttgaa
     1321 caaacaactg gataggacca tttccaactg ctactttctc aactttattg gttgtcagat
     1381 caacgattgc taacgcattt tctccattta atgttactaa gagcgtttgt ccatctgagg
     1441 taataccagt tgtagctgga gtactgccaa cagttattct attaacttct tccatacttt
     1501 ctaaatcaag gacgcttaca gtgtcttcac ccatattggc aacgtaagcc gtttttccgt
     1561 cttcagcgat tctaaaaccg tgtggacctt cgcctgttgg gattgtttca accacttcat
     1621 aagtagatac atcaatgact gaaacagtgt tctctccatt attggaaact gctgcatagt
     1681 tgccatcgtt agtgaaaacg atatgagcag ggtgactccc tacttcaact tctgcaagtt
     1741 tttcacctgt ttcagcatca taaaaagctg caataccttt ttcatcgtca ctttcattac
     1801 tcatactagt atcactatct tcgtcctgtc ccatctccat actttcattg ctcatactgg
     1861 tatcactatc ttcgtcgtgt tccatctcca tactttcatt actacctgaa ctgtcttcag
     1921 catgcccctc gctgggaaca attgttacac caatgacttt ttcatcaggt gaaatttgta
     1981 cattatgtgc ggatccatcg atggcaatcg tatccacaac ttcgtgcgtt tgagcatcta
     2041 cttttgtaac acttccagta tcggaagtgt aataggcttc tgtttgttcc ttattttctt
     2101 ctttagaact agattgagac tcactagttt cttgtgacgt tgatacaatt gcttgttgag
     2161 aagaattttg actacttgtt gactcttctt gaccacaagc tgcaagcaag agacccactg
     2221 acgacaatag tgctaaagtg attctttttt tcattttaat atcccctttt ttactacgta
     2281 ctttttactt ttcgtactat ttttattgct ctataattgc ctgaattatt cataattttt
     2341 ccgtaggttt ctgaaaagat tgtggtactg cgttcctgat ttctcttctt tactttcatg
     2401 gtggtgcata atttatcccc ttgctttgat ttattttatg tttctgtttt tataataata
     2461 tatgaatgtg ttgattttat gtagaaaaaa accatatcaa aaagccaaga tttgtatatg
     2521 atcttggctt tttgatatga atagttcatt tccggaatat atttttgaga aaactcagtt
     2581 cgagcatgaa acttcagact tacccaacta acttttcaat attttcttct tttgttggta
     2641 ttcttcgtct ggttctgttg cgaagtttga aaatcaaacg cgccctctct gaactccaaa
     2701 tatcttaggc tgttattccc attaatacct tgatttcagt agacaccgaa aagccgaaga
     2761 gcgttccatt tcttcggt
//
LOCUS       DASJRQ010000181         2777 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJRQ010000181 DASJRQ010000000
VERSION     DASJRQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.187"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..564
                     /locus_tag="U4A80_002738"
     CDS             118..564
                     /locus_tag="U4A80_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1976328.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            837..2189
                     /locus_tag="U4A80_002739"
     CDS             837..2189
                     /locus_tag="U4A80_002739"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1976329.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2360..2647
                     /locus_tag="U4A80_002740"
     CDS             2360..2647
                     /locus_tag="U4A80_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976330.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          975 a          453 c          547 g          802 t
ORIGIN      
        1 tgttttacgt cttttttcat taaaatacaa taaaattgaa ttttcttttc atgttaagtg
       61 gattttaaaa tgtaaaaata gtatgataag acgagaataa caacgtgggg tgtttacatg
      121 gaaaataagt tagtcgaaga ggttatcgaa aagttaaaaa aagagaacat tcgaatcaca
      181 cctcaacggt acgcggtttt agaatatttg attgaacatc attcccatcc aacggctgat
      241 gagatttata aagcacttga acatcggttt ccaaatatga gtgttgcgac tgtttacaat
      301 aaccttcgct tatttacttc gattggattt gttcaagaaa tgaagtacgg cgatgcttct
      361 agccgatttg atttcagttc aaagcgtcat tatcatgtaa tttgtgaaaa ctgcggtaag
      421 attgttgatt ttcattaccc agggcttgaa gatatagaaa tggctgcaag cagactgact
      481 gattttgaga ttaatgaaca tcggttggaa ttgtatggac tctgtccgga ttgtaaagag
      541 tcagcaaaaa aggcgcaaat gtaaaaacag tttctacaag tgttatacct aaaaaaagct
      601 agctgtgtaa aaaaaacgct agctttttta tttttttgtc ttttattcac aatcttagat
      661 ataaaagtga atattattaa aataagaatc ttgtagatat tgggttctta tgcttgaaaa
      721 ggaatgaaac tacaaacaaa cctttgtgaa ttcacaagca ttaaactgtt gtatccgttt
      781 tatttctgtt atagtagttt tgtcgaagga caaaatgtta atcggaggga ccataaatga
      841 aagtagtagt aattggctgt actcatgcag gtactgcagc tgtaaaaagt attttaaaaa
      901 atcatccaga agcagaagtg acagtatatg aaagaaatga caatatttct ttcttatctt
      961 gcggtatcgc actttatgtc ggcggagtag taaaggaccc agcaggcttg ttctactcaa
     1021 atcctgaaga attatcttca ttaggagcaa cagttaaaat ggaacacgat gtaacagacg
     1081 tcgatacaga aaataaaaca gtaacggcaa aagatctgaa aacaggagaa acagaaacgg
     1141 tttcttatga taaattagtg atgacaactg gttcatggcc tattatccca ccgattagtg
     1201 gaatcgatgc gaaaaatgta ctcctatgca aaaactacaa tcaagcaaat gaaattattg
     1261 cacaagcgaa agaagcaaaa cgtgtagtaa ttgttggtgg tggatatatc ggtattgaat
     1321 tagtagaagc ttttgtcgaa tctggaaaac aagtgacatt gattgatgga ttagatcgca
     1381 tcttgaataa atatctagat aaaccattca cagatatctt agaaaaagaa ttgacagacc
     1441 gcggtgttac tcttgcgcta ggcgaaaatg ttactgaatt tatcacagat gaagaaggaa
     1501 aagtgaaaca agtagctaca ccaacagata catttgatac agatatggtc attttatgtg
     1561 tgggcttccg tccaaataca aaactgatcg aaggaaaagt agatacactg ccaaacgggg
     1621 caatcaaagt gaatgaatac atgcagacaa gtaatccaga catttttgca gctggagact
     1681 ctgcagtagt caactacaat ccaagtggca cgcaaaacta tattcctttg gcaacaaatg
     1741 ccgttcgtca aggtttgctt gtgggaaata acttgacaga tcataaaatg gcgtatcgtg
     1801 gtactcaagg aacttcagga ctttacctat ttggctggac gatcggttcg acaggtgtga
     1861 ccaaagaaag cgcaactttg aacgatctag aagtccaagc gactgtattt gaagataatt
     1921 atcgtccaga gttcatgcca actactgaaa aagtaatgat ggagctagtc tatgaaaaag
     1981 gaacgaatcg tatcgtaggt gctcagttca tgtccaaata cgacattaca caatcagcaa
     2041 ataccatgtc tttagctgtt caaaataaaa tgactgtaga agatctcgca ctttcagatt
     2101 tcttcttcca accacatttt gaccgcccat ggaattactt gaacctactt gcacaagcag
     2161 cgctaggcga aatcgaaact gcacaataaa gaaaaagggt tgtgacacaa ttttgtcacg
     2221 accctctgtc tttttttgtg aaaagtacaa ttagttgtct ttcttcgaat tttttgtcac
     2281 aataagcagc aaagataaag ctgttacata aaagaacaag cagctttatt tgattttgaa
     2341 aagaaaggtg agaaagaaga tgaataaaga atattttgtc caacaagatg catttttaga
     2401 agattataaa agtgcttcga agaaattaac gatcaatatt gatgagttgg cagatcaagt
     2461 actctctcag ttgaatgaaa cgcaagagga atattacacg atgccagcaa atgacgccaa
     2521 agataatgtt tctcatcgtt ttcctttcag tagacgcatc tactcagatg aaaatgacgg
     2581 cacattgaat acagagtaca catatgaagg tcagccatat gtgctagaaa ctgatgatga
     2641 tctttaaaga cagtttttga aaaggccgct catccttaag tgatgacaaa aaatcatcac
     2701 aattaccgat gactgtctct ttactatctt ttaatcagaa aaaggggtta tgacaagctt
     2761 tgtcaacccc ttttttt
//
LOCUS       DASJRQ010000182         2743 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJRQ010000182 DASJRQ010000000
VERSION     DASJRQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.188"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..407
                     /locus_tag="U4A80_002741"
     CDS             12..407
                     /locus_tag="U4A80_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976331.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            732..1349
                     /locus_tag="U4A80_002742"
     CDS             732..1349
                     /locus_tag="U4A80_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976332.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1390..1689
                     /locus_tag="U4A80_002743"
     CDS             1390..1689
                     /locus_tag="U4A80_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1976333.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1723..1890
                     /locus_tag="U4A80_002744"
     CDS             1723..1890
                     /locus_tag="U4A80_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1976334.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1935..2540)
                     /locus_tag="U4A80_002745"
     CDS             complement(1935..2540)
                     /locus_tag="U4A80_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976335.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          964 a          410 c          503 g          866 t
ORIGIN      
        1 tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata
       61 aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac
      121 ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga
      181 gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag
      241 taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg
      301 acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat
      361 ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa
      421 attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca
      481 taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt
      541 atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt
      601 atactttttg acatgccttg attttctttg tttatgtatg gcataataac cacataatac
      661 aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt
      721 agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg
      781 aacgacaact gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa
      841 aagccagcgg caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa
      901 aaggagattt actctatcta caatcattag atagactagg cagaaataaa caaatgattt
      961 tagaagaatg gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc
     1021 ctctgttaga tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact
     1081 taattctgca attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc
     1141 aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg
     1201 ctttacctcc tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag
     1261 tctatgctat ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat
     1321 atgaacatac cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag
     1381 agtttgaaaa tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg
     1441 acacgaaccc aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt
     1501 acttcaaaaa caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct
     1561 ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc
     1621 attttatcag aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg
     1681 tatttataat cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat
     1741 gatcgttggg gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt
     1801 gactgtgggg aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc
     1861 catcgccgat acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg
     1921 aatgtagtat atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag
     1981 atcccgtttt cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg
     2041 gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt
     2101 cccttatatc ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt
     2161 ttctcacgct cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg
     2221 gtatccagga gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac
     2281 gctacttttt tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct
     2341 aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac ggatataatt tagtaatttt
     2401 tgaaactcag gacgctcagt atctttacca ctggcttttt cttggaataa tttatcaatg
     2461 ttaagtcctt ccatcattgc caattgtcga tccaaatttt gatcttgtga agatactcga
     2521 atatagccaa tttttcccat aaaaaactcc tttttaaaag tgatataatt tacatgtaaa
     2581 atttacttgt agttttactg ctatcatagc atttttaaaa ataaatttta tgtagactta
     2641 aaatttactt catggagggt ttaaatggat gaaatcaaaa gtaacaaaaa agcatttggc
     2701 atttatttag gagaacctag tacagaacaa ttaaaccaat ttt
//
LOCUS       DASJRQ010000183         2726 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJRQ010000183 DASJRQ010000000
VERSION     DASJRQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.189"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..416)
                     /locus_tag="U4A80_002746"
     CDS             complement(42..416)
                     /locus_tag="U4A80_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1976336.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
     gene            complement(572..922)
                     /locus_tag="U4A80_002747"
     CDS             complement(572..922)
                     /locus_tag="U4A80_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976337.1"
                     /translation="MSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSDKFRNYI
                     ADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIG
                     IFEKNTSEEKNLKL"
     gene            994..1506
                     /locus_tag="U4A80_002748"
     CDS             994..1506
                     /locus_tag="U4A80_002748"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976338.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1503..2540
                     /locus_tag="U4A80_002749"
     CDS             1503..2540
                     /locus_tag="U4A80_002749"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1976339.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          900 a          533 c          483 g          810 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccacatctg gcattaagaa aggatatgaa gcctttgtct tttcatgtat taactttgac
      121 ttttcaattg cttccttgtt ttcaccgtta tcatcaacag gatctgtaac aactccaacg
      181 atatttacac ccttgctttt catttcattc tgaacctcaa ccaaatcagg aatttccttt
      241 acacaagctg tacaccaagt tgcaaataca tttaccatag taagatcata cttctcaaaa
      301 tctttacttg taaaatcctt gccatttatg tcctttgttg gtagtttacg taaatcttta
      361 accgattctt tgttaaatgc ttctgtattt tctaaatcag ttttttctga tagcacaaaa
      421 ccatttttag gacgttcttt tttatctata atttgaatct cagttctttt taattcatcc
      481 agaagattac tttcagctcc gctatttgtg cttaaatagc aatcgtattt tccatcactt
      541 gaaactccta ttttagtatg agtatcacat tttataattt tagatttttt tcttctgatg
      601 tatttttttc aaatatacca atagtcccaa ttctttcaag ctcattttgc caatttttat
      661 atccatcgcc catcttttct ataactgcat tcttttgttc ttctgtcatt ttctcaaatg
      721 tcaaaatagc atactttagt tctttatcta taggactttg atcatctaac atcgctatct
      781 ttttatcagc tatataattt ctaaatttgt ctgagagttt aaatttaagc cctaaatatt
      841 cataaacata ttctttcttt gcctccaatg tataatctga tggcttaaaa gtttcatttt
      901 tgccatttgc attaattgac atgcccccta tgttaggcta gttgtcgtat aggataataa
      961 gttataataa agactatacg gaggatgatt accatgacaa ataaaaacaa tgaacgttac
     1021 tctaccgaac tgaaagcgac agccatcaag aaaatgatgc cacccgaaaa tagggggatt
     1081 cgccaattaa gtcaaacttt gggcattcca gaagcgactc tttattcttg gcgtaaaaaa
     1141 gctagaattc aagggaatac tacacccgga acgaaaaaaa atgtcgaaaa atggtcaagt
     1201 gaagataaat ttttaattgt gatggaatca ttctcaatga accaagtaga acttgcggaa
     1261 tattgccgaa agaagggctt atttaaagaa caaattgagg cttggaagaa aacgtgctta
     1321 tctgcgaatg aacaggaaga aaatcgaacg agagaacttg caactgaact aaaagaagag
     1381 aaaaaacaag cgcgtcaatt agagaaagaa ttacgtcata aggaaaaagc attagcggaa
     1441 gccgctgctc tcttattact aagaaaaaaa gcacacgcga tttgggggga ccaagaggaa
     1501 gaatgatttc atcttcagat cgcaaacaag ctgttgaact tattcaagaa gccaatcgca
     1561 atggggcccg tctcacctat gcctgtaatg agctgaatat tagcgttcga acctacgaac
     1621 gatggacgcg tgaaggtacg atagcgcatg atcaacgccc tttggcgaaa cgtcctgtac
     1681 ccaaaaataa actgacggat caagaacgcg aaaaaataat agaaaccgtt tcaaaaaaag
     1741 aatttatgaa tttgccacct agccaaattg taccaaaact tgcggattgc tcgatttata
     1801 ttgcatcaga gtctagcttc tatcgtgtat tgcgagaaaa aaatatgcaa catcaccgtg
     1861 gccgaagtca agtttcacaa aaacgcatac cgccaagtca ccttgcgacg aagccaaatg
     1921 aagtttggac ttgggatatt acttggctaa aaggtccaat aaaaggactc ttttaccggt
     1981 tatatctcat cattgattta tttagccgta aaatggtagg ctgggagatt tgggaatcag
     2041 aagaagctcg tcatgccgaa aaacttatta aacagacggt gcttaaggaa cgtatccaag
     2101 gacagccgtt ggttttacac tccgataatg gcagccctat gaaagccgcg acttttcaag
     2161 tcttactcga aaaattagga attcaaagtt cttattcaag accacgagtg agtaatgaca
     2221 atccttattc ggaagcggct ttccgaacgt taaaatatcg accagaatat cccaataacg
     2281 gctttaaaac aatagatgat gctcgaaaat ggacgaaaga gtttgtcgat tggtataacg
     2341 attctcattt gcatagtggg attcaatttg tgtctcctgc agcgtgtcat gaaggacgac
     2401 atctcgctat tttagagaaa cgaaagcagg tttacgagga cgctaaaaag cgccatcctg
     2461 aacgctggtc aaaaggtgtt cgaaattgga ctccacatca atcggtggcg ttgaatcctt
     2521 taaaagaaac agaaagttaa caggagcata atctttcaag attatcaaat aaccctcgtc
     2581 acccaatagg ggaaaaagtt attcttcccc tattggataa cgaggccaac cgaccatagg
     2641 gaggttctgc tttttgatag gtggaaaata aaaatacgac aactatattg acaaacaccg
     2701 accatcgtcc agtaaagaga cgcaat
//
LOCUS       DASJRQ010000184         2691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJRQ010000184 DASJRQ010000000
VERSION     DASJRQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.190"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..471
                     /locus_tag="U4A80_002750"
     CDS             <1..471
                     /locus_tag="U4A80_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976340.1"
                     /translation="KNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLEN
                     KEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPV
                     KEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            646..1479
                     /gene="msrA"
                     /locus_tag="U4A80_002751"
     CDS             646..1479
                     /gene="msrA"
                     /locus_tag="U4A80_002751"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1976341.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1592..2548
                     /locus_tag="U4A80_002752"
     CDS             1592..2548
                     /locus_tag="U4A80_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1976342.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          988 a          429 c          524 g          750 t
ORIGIN      
        1 aaaaatttca atcaaaaaat aatttctcag ccgggacata ttgttgtaaa caaagaaaca
       61 gtagaggcgt ataccactaa aggaatggat tattttttat atgattattc tcctatcgct
      121 tcattggaga ataaggagca tataataaaa cacgaagcaa agattggcag tcaatgggta
      181 aaccttcctt ataaaacaac tggtagttct atcatatata gagtaactaa tgagaagagc
      241 caagtaatca cacttcctgt atatgcttat ttaggaacga atgtagaaat aaatcatcaa
      301 cctgtaaagg agcaggtgaa tgctaatgga ttggtacaaa taagcggagt caaaggagtt
      361 gctgatatta aagtaagcac aagttataca gtaacgacta ttatttctta tcttgttaca
      421 ttaactgcat atttatgttt attttttaaa atagttttcc ataaaaggta aattaacttt
      481 tcatttataa aataaattga ttaaaaagga gaatctgtcg ataaggattc tctctttttt
      541 atacgaaaaa tctgtagggg gatgaagtaa tcgcttattt cctcacttta tgataaatta
      601 cagctaaagt cactagcttg tcttcttcgg aaaggaggag aatctatgga tgaggaacaa
      661 atattcattc aaacacttta taatctgatt ttgaatccga atacacgaga ttgggaacga
      721 aaagtattga ttcagacaaa gaatgacata agagaaaata tatccgttaa agaacagtta
      781 tcaaaactag aagcaacact acgtccatta gctatccgaa tgaatctgac gcctgatgta
      841 atggactttt atttattgat tacggaagga tttgataagg agcaaaaata tgacttttcc
      901 aagcatgcca tgcaagatgc tgactatcaa gaacgtgcag tatttgcagg gggatgtttc
      961 tggtgtatgg tcgagccgtt tgaatcgaaa aaagggatcc tatctgtttt gtcaggatac
     1021 acgggcggac atgttgaaaa accaaattat gatcaagtaa gtggtggtta cacaggtcat
     1081 gtggaagctg tggagatcat ttatgacaca agagaaataa gttatagcga gttgctgaca
     1141 atctattggc aaattactga tcccacagat acatttggac aatttcaaga tagagggaag
     1201 caatatcgtc cagtgatttt ttatcaaggc gaacgacaaa aagaattagc ggaacaatca
     1261 aaacagaaac tagacagttc aggaactttc caccaaccaa tcgtcacaaa aatcgaacca
     1321 gccggtacgt tttggccagc agaaaactat catcagcaat tttacaaaaa acaaccgaaa
     1381 cgttataaaa aaatccagca agcacgtaat caatttctga tctatcaacg agtaaaaaac
     1441 aaatggcaaa aaaatatacg aaaaaatcat tttgattaac tccttttctt taaaatgtag
     1501 taaactaaaa atagaaaaaa gaaagcgttt taaaaagatt tgatgaggag gagaaaaatt
     1561 gtatattgca gatagtgcac gttatgaaaa aatggaatat cgtaaatccg gtcattcagg
     1621 tttgaaatta ccgattttat ctctcggatt atggcaaaat tttggtgatt atgatcctat
     1681 ccataatcaa agagagattt taagaggcgc attcgacatg ggaattaccc atttcgacct
     1741 tgcgaataat tatggaggcc ctgctggagc tgcagagaaa aactttgggc gtatttttag
     1801 agaagatttt caagcctacc gtgatgaact gattatttct agcaaagcag gttatcatat
     1861 gtgggaaggg ccatatgggg aatggggctc tcgtaaatcc atcatttcaa gttgtgacca
     1921 aagtctccaa agaatggggc tagattatgt tgacatcttc tatcatcatc gcccagaccc
     1981 tgatacacct ttggaagaaa cagcagaagc tttgatgcaa cttgtaagac aaggaaaagc
     2041 attatatatt ggtatatcca actataatgg agaagataca aaaaaaatga ctgaaatttt
     2101 aaaaagaaag gaagcaccgt tcattattca tcagatgcgc tacaatatgt tttctcgtgc
     2161 actactagaa gacgatctat ctccagtatt agaagaagaa gggctagggg caatcacgtt
     2221 cagtccgctt gctcaagggt tgctgacaaa tcgttatcta catggtattc ctgaagattc
     2281 tagagcacat cgtaaggaaa taccattcct ttcagaagaa caagttggat caactctgga
     2341 aaaaatcaaa gcactgcaaa caatcgccgt atcacgagga caatcattag cgcaaatggc
     2401 ccttgcatgg aatttgcgac aaaaatctgt tacaagtgta ctggtcggag caagtcggtt
     2461 aagtcagtta caagaaagtg ttcgaatgat ggataattta gatttctcac cagaagaaga
     2521 attgagaatc gaccaaattc tagaatagaa acgatgtaat caaacttttt atccatatat
     2581 taaaaaaaac aaaagatgac atcaagaatc gaatgaagat cgtctgttat tgaacatcat
     2641 attttttgtc gttcaaaata aaaatgatcg aaggctagga gtgaatttta t
//
LOCUS       DASJRQ010000185         2665 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJRQ010000185 DASJRQ010000000
VERSION     DASJRQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.191"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            36..359
                     /locus_tag="U4A80_002753"
     CDS             36..359
                     /locus_tag="U4A80_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976343.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            523..777
                     /locus_tag="U4A80_002754"
     CDS             523..777
                     /locus_tag="U4A80_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976344.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            847..1092
                     /locus_tag="U4A80_002755"
     CDS             847..1092
                     /locus_tag="U4A80_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976345.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1168..1533
                     /locus_tag="U4A80_002756"
     CDS             1168..1533
                     /locus_tag="U4A80_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976346.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1594..2157)
                     /locus_tag="U4A80_002757"
     CDS             complement(1594..2157)
                     /locus_tag="U4A80_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976347.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          784 a          473 c          421 g          987 t
ORIGIN      
        1 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
       61 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
      121 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
      181 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
      241 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
      301 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
      361 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
      421 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
      481 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
      541 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
      601 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
      661 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
      721 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
      781 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
      841 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
      901 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
      961 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
     1021 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
     1081 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
     1141 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
     1201 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
     1261 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
     1321 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
     1381 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
     1441 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
     1501 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
     1561 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
     1621 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
     1681 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
     1741 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
     1801 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
     1861 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
     1921 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
     1981 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
     2041 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
     2101 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
     2161 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
     2221 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
     2281 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
     2341 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
     2401 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
     2461 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
     2521 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
     2581 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
     2641 ttcagcccat tcaacgacca atgaa
//
LOCUS       DASJRQ010000186         2562 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJRQ010000186 DASJRQ010000000
VERSION     DASJRQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.192"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..461)
                     /locus_tag="U4A80_002758"
     CDS             complement(<1..461)
                     /locus_tag="U4A80_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1976348.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(747..1034)
                     /locus_tag="U4A80_002759"
     CDS             complement(747..1034)
                     /locus_tag="U4A80_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1976349.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1024..1353)
                     /locus_tag="U4A80_002760"
     CDS             complement(1024..1353)
                     /locus_tag="U4A80_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1976350.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1430..1702)
                     /locus_tag="U4A80_002761"
     CDS             complement(1430..1702)
                     /locus_tag="U4A80_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1976351.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1702..1965)
                     /locus_tag="U4A80_002762"
     CDS             complement(1702..1965)
                     /locus_tag="U4A80_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1976352.1"
                     /translation="MAKTVLNPSTARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(2071..2448)
                     /locus_tag="U4A80_002763"
     CDS             complement(2071..2448)
                     /locus_tag="U4A80_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976353.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGNG"
BASE COUNT          787 a          498 c          319 g          958 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
      781 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
      841 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
      901 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
      961 tctttcaatg gcttctcttt tagctttttt aaatacttaa tgacatgttt tgaaggaacg
     1021 atttcataag ccaatttctg ttgccaactc ctctttagtc attactttag aattagtaag
     1081 tgtatcttca gcaatatctc taaaggaaga atgcattttg ttttttctat tcttaaattc
     1141 tacaagaatc tcatctttat cgtagccttc tgcaattata tcagacaaaa tatcttcgct
     1201 aaaatcataa aattcttttt gtggttcaac aaattcataa agaataccat tctctaccaa
     1261 ctttgcttca acttcgactc cattaggaac attaaaatct ttaggtactg tcaacatgat
     1321 tgaattgcct tgttttcttg tttttgtaat catttaaatc cctcatttct aacagtatgt
     1381 aattacttag taaatacatt ataccacgag ataatgtatt tacaagtaat tactgtgact
     1441 cgtaatgcgt ccttgctgaa taaatttcaa ccacgtgttc atcttcatct accttataaa
     1501 caacccggtg ttgtctattc aatcttctag aatacctacc ttcatgtgta ggttttaact
     1561 tctcaaatga ttgggtaggg agatagggat cttcctttaa agtctgaata accttctcaa
     1621 attgaggttt caaattagac tgtttgattt tctttaaatc agctttagcc gagtttttaa
     1681 tcatcacaga atagttattc attataagtt atcccaatca atttcatcaa cattagtaaa
     1741 tccactatca tctttttctc gcttacgaac aacacccaaa gtgcctattt gctcaagtaa
     1801 tagcgtttct ttgattgctt tccaatcatc caaaccaata agcaccgcat tattttcact
     1861 gcgctcacta atgatttcaa tttccgtatg attctcattt acatctttca acaacttata
     1921 aaaatttttc cgtgctgtgc taggatttaa gacagttttt gccatatcta ctccccctct
     1981 aatttatatc tccattctaa cgtacttaat taagtacgta aattcgattg ttttgtttaa
     2041 attggagtac caaaacagca ttgtaaagtg ttatccgttt cctttatttt tattaacaag
     2101 ttttatcaca ggcttcatat atttgattat atcttcatct tctacataga tatctgttat
     2161 cattacgaca tctgtactat ttcgattgag tactttcact tgatctccag cattgatttt
     2221 ccctctaaat cttttaggaa tgaaccaact atacatatga ttagcatcac caaaatgtgc
     2281 ttttatcgca ttctcaggat ataacttatt caatagttca ttcttttgaa ttttcaaaac
     2341 ttcaaatcta tacttatcat cttctgctaa tctaaataaa tcttctaaac gcatgttttc
     2401 taactgtaag ttttgaaaat cttccggtat aaattttcta tatttcatta ataatgaacg
     2461 cccggcccga gttattataa tctggccatt ttggtcatct ctataaagaa ttccgtaacc
     2521 catcaacgcc aatagcacac gtgacgtttt gttacttgta at
//
LOCUS       DASJRQ010000187         2475 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJRQ010000187 DASJRQ010000000
VERSION     DASJRQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.194"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..903
                     /locus_tag="U4A80_002764"
     CDS             <1..903
                     /locus_tag="U4A80_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1976354.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..1824
                     /locus_tag="U4A80_002765"
     CDS             916..1824
                     /locus_tag="U4A80_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN1976355.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1824..2441
                     /locus_tag="U4A80_002766"
     CDS             1824..2441
                     /locus_tag="U4A80_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976356.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          859 a          346 c          555 g          715 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttcaga taatcacgaa gtcgaaaaaa ttgaaacagt agaatttcca
     1741 attgaagaag gagctttgat tattgcaact gttgatgatc caaaacagaa tgttttcact
     1801 cttgagaaag ttggggtgat ttgatggtta aagtaaagag aatgatggag actgatgaat
     1861 ctggtgttaa acgtcaattt atgccaatta cacatgtttc tgcagtttta ggcttagaaa
     1921 aaatgatatc aggacaatca aaagttcttt cggttaatgg taaatatggt gcagtcattt
     1981 taacgaaagc tgatttagga ttagagaatg caattactga actaccttat gcaagcgaga
     2041 caagcgatgg tattcttact gctgaaatgt ttcaaaagat cgtaaatgga gaaggtggaa
     2101 catatatttt gccgattgct actcctgaac aattaggagg tataaaagtt ggggaactct
     2161 tagaagttac tgaagaaggt gttttatctg caactaagca aacagacttc aatttctctg
     2221 aagaattaaa gggaaaatta gaatctctaa aaatattaaa agcaggtgca aatatatcaa
     2281 ttgcagaaga tggaacaatt agttctacag gtggtagtgg gactggtggt gttaatcagt
     2341 catatgtaga tcaaaaattc caagaagctg taaatcaagc agaaaattac acaaacgaaa
     2401 gaattccaaa ttttactttt gaaaaaatcg gggaggtata gaaaatgaca gatatcgtaa
     2461 aaataaaaca aagcg
//
LOCUS       DASJRQ010000188         2398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJRQ010000188 DASJRQ010000000
VERSION     DASJRQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.195"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4A80_002767"
     CDS             207..2069
                     /locus_tag="U4A80_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1976357.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U4A80_002768"
     CDS             2069..>2398
                     /locus_tag="U4A80_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1976358.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASJRQ010000189         2344 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJRQ010000189 DASJRQ010000000
VERSION     DASJRQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.196"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            425..1384
                     /locus_tag="U4A80_002769"
     CDS             425..1384
                     /locus_tag="U4A80_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1976359.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1371..1976)
                     /locus_tag="U4A80_002770"
     CDS             complement(1371..1976)
                     /locus_tag="U4A80_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976360.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2216..>2344)
                     /locus_tag="U4A80_002771"
     CDS             complement(2216..>2344)
                     /locus_tag="U4A80_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976361.1"
                     /translation="GKKSLEIRKWTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          768 a          400 c          371 g          805 t
ORIGIN      
        1 tacaaaatat gtgatatagg aagtttagtt attgttctca taatgccatc ctgtttttag
       61 attatctagt aatttttttg ttatttaaaa ttaggagcag tgccaattgt tttaatttta
      121 aaggcttttt ttatatcttc taaattagaa gatatttgta tatcaatatg gaatatatta
      181 ccttttttgt atttttttat gttatcttta gctaaaacat caaatacatg tccttgaaat
      241 tgtatcgagg cagctggatt tagtttaaat tgaggaaact ttaccacagc tgattcaaaa
      301 cacttgtcac atttttttat ttttttacct tcgccgattg taaaattaag ctagacaact
      361 aaaaaagtca tttgtgctac actcaaaata gttccgtcga aagaattata aggagtgagt
      421 acaaatgacc tatacccatc ttacaccaac tgaactcgta atgatagaag cttatttcaa
      481 tcaatcacac tctgtttcaa aagtagccca tctggtacaa cgttctcgac aaaccattta
      541 taatgtctat cgttttctta aatctggtgg aagcgcaata agctactatg aacaatataa
      601 gaaaaacaaa cgtaactgcg gaagacgtcc gattgtgtta ccagatgggc aacaagaata
      661 cattcaaaaa aaggtggctc aagggtggac tccagatgtc cttattggtc gagcagagtt
      721 tcctattgct tgttcggtac gtacactata tcggatgttt aaagataaaa cctttgaccc
      781 tcacgattta ccaatgaaag ggaaaagaaa accaaacgga cataaagaaa aaagaggaaa
      841 acaagctttt cgccgttcga ttcaggatag aacaactgat tatatacagt tcaatcatga
      901 gtttggtcac ctagaaggtg acaccattgt cggtggaaaa cataaaagtg cggtgatcac
      961 gttagttgag cgattatcta aagtgatcat caccttgaaa cccgctggta ggcaagctat
     1021 agatattgag aatagactga atcaatggtt tcaatctgta ccgaaacact tgtttaaatc
     1081 catcacattt gattgtggaa aagagttctc gaattggaaa aagattagta ataccaatga
     1141 tatttcgatt tatttcgctg atccaggaac accttctcaa cgaggactaa atgaacattc
     1201 gaatggctta ttacgtaaag atggcttata taaagctatg gattttaata aggtatccga
     1261 aacctttatc caatcggtgg catcaaaacg aaatcatatt cctagaaaat cattacacta
     1321 tcgcacacca ttggaagtat ttttgagtta cgtaggtaaa gatgatttgt ctagcttaat
     1381 ttgacaaacg aaactttgta aaaatattcg attgaatatt ctatttggca cgactcactt
     1441 ggcagtatat gtatgtcaat atttttcatc tgtttcatag gtatttcaaa atctaaacta
     1501 ttaaaaacat ataattccct gtaattattt acatctaact ctactaaatt tatctttgga
     1561 acaatagatc ctccacccat tccaccataa taaaaaaaat cttgttctct atcaaaatag
     1621 cacatattaa gctcagtttg tttatcatcc tcaataattt tataaatcaa acctatataa
     1681 tcatgaatgc tggcaaaaat tttttttaag tcaagttcta aatcttcttc gaaaacacat
     1741 ttaatttctc ctactgataa ttgatactct atattacatg acttaattag ttttttagat
     1801 cccccctcca gataggcata tgtttcaata attatgttct ttttattgct tggcaaattt
     1861 ccgttattta ttgcataaat taaaaatgac tgtctattta gcccataatc taatacaact
     1921 tcttcatact catatttctt taaatattta atatgaaaat atgctgaatt aatcaaaact
     1981 cgagtgttac tgttgttatt tattgttgtt ctattgtgta taaaattgta taggttgtca
     2041 tattttcttg gtttagtgaa ctactcacca cttagctaaa cctaacggtt tcttacgttt
     2101 gaagtgggag cttcttggga agagagcata cttgatagcg tattacttta ctaacagagg
     2161 tgcctcttat ttaccaagct atccccgtag ttcctacggt tcttgtttat ttgatctaag
     2221 ccaatcccat agagtgtagc cttagacctt cggtcaggat attgatacta gcgttgatat
     2281 ctcggtcatg atgagtatga caaatagggc aagtccattt tcgaatttcg agagatttct
     2341 tgcc
//
LOCUS       DASJRQ010000190         2329 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJRQ010000190 DASJRQ010000000
VERSION     DASJRQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.197"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..796
                     /locus_tag="U4A80_002772"
     CDS             <1..796
                     /locus_tag="U4A80_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1976362.1"
                     /translation="QGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTP
                     DVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHE
                     REKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLN
                     QWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLR
                     RDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          852 a          309 c          466 g          702 t
ORIGIN      
        1 gcaaggaaaa tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg
       61 tagacgtccg cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca
      121 aggatggaca cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg
      181 taccatttat cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg
      241 caagcgtaaa ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat
      301 tcatgaacgt gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga
      361 cactatcgta ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac gattatcaaa
      421 agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa
      481 tcaatggttt gaatctgtac cgaaaaacct attcaaatcc atcacttttg attgtggaaa
      541 ggaattttca aattggaaac agatcagtaa tgtcaatgat attgccattt atttcgctga
      601 tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt tacgtagaga
      661 tggtttattg aaatctatgg atttcaattc agtagatgaa ttttttattc aatctgtcgc
      721 atctaaacga aataatattc ctagaaaatc actggattat cgaacgatta tcgaacacct
      781 ttggaagtat ttttgagtta cgtaagtatt gatgatctgt ctaatttaat ttgacaatta
      841 agaaagtatt aacttgtttt gtgaaaaaga gaatttgaaa aacgattggc gaacaaatgc
      901 tcggtaggta tggtataaat acagagctaa ttggtgtgat gaaatcagat attcgcgtga
      961 gattcagtaa agtaggagtt cgaaaacagc taggtgacaa acagtattgc tttggtactt
     1021 cggaatggtt tatcaaattc ttatcaacag ttcacttctc tttatgtttg agtgatgatt
     1081 cgttaggtta ttgcttggaa agaccggatg agagtagaag agtttaattt tataaagtga
     1141 aaagaggtaa tgtacgatat acgtagggct gaaccaatag aggataagca aacgatgtta
     1201 caggcgcaaa attcatagct tactgttttg gagataacag aagaggaaat aatgcagaag
     1261 taaggcgaaa aaaggacgga agcctaacag catatgaaat aaagaaaaat attataacta
     1321 ctgataaatc ggtattgagc aaagtactaa taggagtcaa aaacattcat aagctatgag
     1381 tgtttcttgg ctactttttt atttttatgt atctacaaaa gtttccaggg gcagaacctg
     1441 tgattattaa tggaagaacc tcaagtattg tcttaaatca ttgttatgaa aatggattaa
     1501 aagagttcat caaggtgtga ttaacaagtt acttttcatt acatcgaatt tttcagaagt
     1561 tgcttatatt ggtgatgata ttaatgattt atcatgtatg acatctgtga agaagcggga
     1621 ggaatagtag aatgtccaaa aaatgccaca agaaatgaga ttgaattatc tgattttatt
     1681 gtaactcaca gtggtggtga cgaatctgtg atagagttta ttgagtggat attggaatcg
     1741 atatagtgta gtagtacaga aaaattatca tacaattaat actaggcata agaatattgg
     1801 catattctag agttaaaagt aactattcga aaaggataag gttagaattt tttgttttgc
     1861 cagtggaaga atataaaaag ggcattggaa attaatatat tagaatgcta agcggagaaa
     1921 aaaatatatg aaaagtacgt ccttgtgatg ggatggaaaa atacacttat ttcaaacaac
     1981 gataatataa ttatgtgaac cgaagtatac tcgcttctaa ttcaatcctc aaatcccgac
     2041 agtcgcgact tcaaaaaagc gtatagtttt aatgtatccc catatgatcc gaaaaatggt
     2101 aatttacaat taacagtttg atgatgttga agatgtggaa atgctgaaat taccatgatg
     2161 agaaacacat taattaaagg gaattaatta actagaatat aatctgattc aatttgaatt
     2221 aagcaaaaaa aggctgagtt ttaaataatc gcgaattgta aaattaagtt agaaaaataa
     2281 aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaa
//
LOCUS       DASJRQ010000191         2322 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJRQ010000191 DASJRQ010000000
VERSION     DASJRQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.198"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..269
                     /locus_tag="U4A80_002773"
                     /pseudo
     CDS             1..269
                     /locus_tag="U4A80_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            808..1008
                     /locus_tag="U4A80_002774"
                     /pseudo
     CDS             808..1008
                     /locus_tag="U4A80_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1495..1674
                     /locus_tag="U4A80_002775"
     CDS             1495..1674
                     /locus_tag="U4A80_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976363.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1720..>2322)
                     /locus_tag="U4A80_002776"
     CDS             complement(1720..>2322)
                     /locus_tag="U4A80_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976364.1"
                     /translation="AKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRPE
                     FQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGNE
                     LLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQK
                     RLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          761 a          388 c          422 g          751 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catttaaaaa gaaaagtgac
      361 agataggaat aaaaacttat taaatgtagt tatgatctgt tatatttcat ttctaatact
      421 aggtcttcca caagcagtgt ttcctgaaat gttttctgga gactggggta ttgtcagcta
      481 ttctatatta acattgattg ctatggtttt aggtacttca ttttataatg cttttgtaag
      541 taagcatgtt gaggaatatg aagtattaat gtacttaaaa gagaaaaaaa caagtaatta
      601 atatgagaac accacgcata gtttttattt tgagatatcc tagaatgaaa cacattaaga
      661 acgaaagcaa agcaaaagac gccagaactt ctactacctt ctaagttttt tcatatttgt
      721 ttgttatgct atacatagat ctgcataatt agacttgcgc tgcccgttat tgaagtttca
      781 ggcagcgctc tttttggggg atcgataatg gaagaagaaa aaatatttga gaaacgctgg
      841 cagctagcat caagtgaaca aagagcaaga tacaataatt tgatcacctc ctaccccact
      901 gttgactaga catataagga aaagaaatac ttactttggc tgtgccagtt agatatagat
      961 acctttgaaa catttgaagt gatcctagat aaaattaaac agaattgatc agaaacaagc
     1021 gaagccttct ctcggttcgc ttgttttgct atttattagt tggctcaggg caactctaag
     1081 ctcgtttgaa agaaaaccga tggataatcc ccttgtttgt accactttag aatacatgga
     1141 tgtacgtatt gccattgact cctattagtt actgaaaaaa aatttaaaca agttgagtat
     1201 tgcagataaa tagctccagt gaggtgaata actgctccac tcaaggcaat agatagctcc
     1261 atccagggca acaaactgct ccactttcat atttaataga tgaataacct tctgtataat
     1321 agaaaggcat acctttcggt atgacaactt atacaggatg ttattttatt ggttcactat
     1381 cgaaaaattt tggagcttta tttttccggt atttctcaac gaacgattag ttcaactgtg
     1441 ggaagctcca gacatacgat tcgagaagtt acttctcgag cagacaggaa gggggtgact
     1501 gaactaaccg ctgatatgag cgatacttgg ctgcaggaat atctttttcc ggaaaaacaa
     1561 acaacagcac gaggttacct tcctgtagac tgggaatatg ttcataaaga actaatgaaa
     1621 aagaatatga cattgaaact ccttcataga gaatatgggg tacatggaac atagaaacga
     1681 aaatgcacgg tagaaaagcg aatgaatgta gtataaattc tacaaatcat tcgctttctt
     1741 tattttatat atggttggtc gagagagacc attttctttg gcaatttctg caactggaac
     1801 tccttgattt aacatgtcca ccacagtttt aaagactaat cgtttttgcg gatctgttga
     1861 gttctcagaa tattcaattg gtcgcccctt atatcgacca gcttttttgg ccaataatac
     1921 tccttgccgc tgacgttcac ggattttctc acgttcgttt tgtgcaatat aacttagcaa
     1981 tgataaaaac atatcgaaca tagcagtatc taggagttca tttccagtat taaaatctaa
     2041 aaactgtgca tctagtattt ttaatgctac ttttttctgt ttcataaagg taactgtatt
     2101 ttttatgtct tcataatctc tgcctaggcg atccagtgat gcaacaatga cacaatcacc
     2161 ttctcggata taatttagta atttttgaaa ctcaggtcgc tggatatctt taccactggc
     2221 tttttcttgg aataatttat caatgttaag gtcttccatc attgccaatt gtcgatctaa
     2281 attttgatct tgtgaagaca ctctaatata tccaattttt gc
//
LOCUS       DASJRQ010000192         2304 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJRQ010000192 DASJRQ010000000
VERSION     DASJRQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.200"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1225)
                     /locus_tag="U4A80_002777"
     CDS             complement(<1..1225)
                     /locus_tag="U4A80_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEN1976365.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGS"
     gene            1483..1719
                     /locus_tag="U4A80_002778"
     CDS             1483..1719
                     /locus_tag="U4A80_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN1976366.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            1738..1953
                     /locus_tag="U4A80_002779"
     CDS             1738..1953
                     /locus_tag="U4A80_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN1976367.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          791 a          376 c          402 g          735 t
ORIGIN      
        1 aagaacctag ccataaaatt aatgcactgc taattaattg aatcaaatgt ttaattcctt
       61 gttgaatatt atcaagcgta accgtacgaa atgatttttt cattaattta acaaattcat
      121 ggtctatacg gttatatact ttctcttcac cgctatatgc tttaattgtt tctattcctt
      181 taagactttc tatgatacta gaatttacag ctgcaccggc tgccatttct tccctgttcg
      241 atttttcata gcttttcacg aaagcgaata ttgcaactac ataaaaaggc aaagagcata
      301 gagtgattaa aaacaacgta ctattctgaa tagctaacgt aaccccaaca agcaacacca
      361 taccaatatc taaaaatatt gataacgtag cacttgctaa agcatcaata atcttactgg
      421 catcaagaaa acgagaaata atttcccctg attttctcgt agcaaaaaaa ttcattggta
      481 aagttaacac atgtttgaaa tactgaagca taattgccat actcattcgc tgtcctaaaa
      541 taataagaag ataactacga ctatattcaa acaatacccg aaataaatag acagcgatca
      601 agccaatgga aattatattt aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa
      661 taccttgaaa ataatatgag cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa
      721 cgatgtggaa tactaatcct ttttgtttcc aaattatagg caaaaaagaa cttaatccag
      781 ctactttttc tttaatagga atataagttt ctctaggcat aggtaataaa agaatacctg
      841 tccattcttt ttgaaacgct tcaatcgttt ttctattttt tccattagcg ggatctgcaa
      901 tccatagtac gtttcccttc gttttataaa ttacaacgta gtgcatatag ctgttatcaa
      961 ccaatatatg tgcaattaat ggtaagataa gatctctttc tttccagatc tcactatctg
     1021 cttgaatagc catacaatca aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg
     1081 aagtaccctc taaatcagtc ccagataggt cccgtaattt atgaatcggg atatcagttt
     1141 taaaatattt taaaatcatc gaaaggcaag caacaccaca atctttttca tcttgttgct
     1201 gcacaaaagg aaatgatttt atcacttatc taacacaact ccttctaaaa aatttttata
     1261 gaaaaattct caatatagct ttaatacagt gaaaatattg tctcataatt ccacaaaaac
     1321 tttgaaaaca gttcttgaac actaactttt tcattcctga atcaaattta ttttggaaat
     1381 tttactattc aagaataaac ttgataagtt tcaggaagat ttttactgtt aatcaaatac
     1441 gtatgttaca gtagcaccgt aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa
     1501 tgtaaaggaa ctaagtattg tggaaatgca caaactgttg gtggaggcaa taatgcttgg
     1561 ggaaaattgg ggcaagttgt aggtggactt accacagggg cagtaggtgg tgcaggttta
     1621 ggaacagcaa tttgcggacc agcttgcggt gttgttggag ggttatatgg agctgtcgct
     1681 ggaggtgctg cagctggctg ggatgctcga aaaaaataaa aaaggagaat aagtagtatg
     1741 catattaaaa acacaaaaac aacatttata ttatcttcag aagagttgaa aaatattcaa
     1801 ggtgggagtg ccgtgggagt tttaggcact actttttctg gggcaacagc aggagtaaaa
     1861 ctttgttcag ccggaggacc ttatgcaata gctgcttgtg gtgtaggtgg agctttatta
     1921 ggtgctggat tcagcatgtg gaccggtgcg taaacaatta aaaaatatca aacctcaaga
     1981 ttggtttgtg tcgattacga acataggtat gttctttatc ttttcaaaag tttggaatgg
     2041 acagaacttt ttactaaagc ttcttgtgct agtttttata attatattaa atttttatct
     2101 tttattaaag tcagataaaa attctttgga aatgtaatgg tagagatatt tttacaaaag
     2161 gacttgcagc gctgtagttg gtgctgcagg aggacctatt tcggccgtta caggaggtat
     2221 tggtaatgta cttgtcggat gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa
     2281 caggaaagta attatctcaa atcc
//
LOCUS       DASJRQ010000193         2243 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJRQ010000193 DASJRQ010000000
VERSION     DASJRQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.201"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..209)
                     /locus_tag="U4A80_002780"
     CDS             complement(<1..209)
                     /locus_tag="U4A80_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976368.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRN"
     gene            complement(583..849)
                     /locus_tag="U4A80_002781"
     CDS             complement(583..849)
                     /locus_tag="U4A80_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1976369.1"
                     /translation="MKSNKSFNKVLELTETALATPEIKKDKNLCEILEKVKASAAKGE
                     FYYDYKKEFQPAISGFTIRNGFSTPKVLLELLAEVKTPKAWSGL"
     gene            complement(854..1018)
                     /locus_tag="U4A80_002782"
     CDS             complement(854..1018)
                     /locus_tag="U4A80_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1976370.1"
                     /translation="MTNFGTKVDAATRSYGNGVYCNNSKCWVNWGEAKENIAGIVISG
                     WASGLAGMGH"
     gene            1600..2211
                     /locus_tag="U4A80_002783"
     CDS             1600..2211
                     /locus_tag="U4A80_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976371.1"
                     /translation="MAKIGYARVSSIDQNLDRQLELLSKCDKIFTDKASGKDTNREGF
                     QKMMEFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGKPKYREDDKRLQLAF
                     KLFLAGSTDKEVEEQTGINRRTFRRYRMKYGITSKLREKNKIK"
BASE COUNT          811 a          363 c          341 g          728 t
ORIGIN      
        1 atatttctac ctaaacgatc taagcgccaa acgacgagtg tatcgccaga gcgaacaaaa
       61 tcaatagcca tttctaatcc tggtcgatta ctttttgccc cactcatatg atcagtaaaa
      121 attttttcac aaccaaattt atttaggctg tcttcttgta aatccaaatt ttgtaaacca
      181 gtagatactc gggcgtatcc tattttcaaa aaaaatcact ccttcattaa ttttattgta
      241 acataacttt ataccaacga tataaatgaa catagatttt gtgatgggtt tgtgaacact
      301 aaaagtatag aaaaatttaa atttaaggta aaaaaatgta gtgtccagaa atgcctgatt
      361 ttatgaacac tttgttcaac agtaactttt gctaatcata caagatccga agattatact
      421 cgctcatttt tccataaaaa agaagccgtt cttttgacga acgacctctc ttttgatttg
      481 tactatccaa atatattgta acattttaat ttctatattc catatgttcc ttaaaactca
      541 attagatact cttacaaaaa atagaatagc acttatcata actcaaagtc ccgaccatgc
      601 tttgggagtt tttacttcag caagcaactc caataaaacc ttcggtgtgg aaaagccgtt
      661 tctaatagtg aatccactaa ttgcaggttg aaattctttc ttgtaatcat aataaaattc
      721 acctttagca gcactagctt ttactttttc taaaatttca catagatttt tatctttttt
      781 aatttctggg gtggctaatg ctgtttcagt taattctaga actttgttga aagatttatt
      841 acttttcata gtattaatgt cccatacctg ccaaaccaga agcccagcca ctaataacga
      901 ttcctgcaat attctcttta gcttctcccc agttaaccca gcatttacta ttattacaat
      961 aaacaccatt accatatgaa cgcgtagctg catcaacttt tgtaccaaaa tttgtcacaa
     1021 ctaacccaaa tattccaata agagctaaac taaataattt ttttctcata aatcaatacc
     1081 tcctttttct aaaataatta taaatgattt ttagaaaaat ttgataatta tatcataaac
     1141 agttgttttg aaagcataat ggcaataaaa aatcattgtt aaaactaatc tcatttgtat
     1201 tcttcgggga attaattcat tccctagaaa atcgtgtgtc acacaataac gcgcataatt
     1261 tcctactttt ctcaaatatt ttgctacttg attttcatat tcttccattt ctggagtata
     1321 ccctattgag atctaatttt atatctatct gaacttaatt aagtgaacta ttgaaagaac
     1381 gctgaaatga gaaattaaaa caggatcaaa accttaaaag ttgttggccc ttctataaag
     1441 ctatagaata tttttcgaat ttttgtgtga caaatactga gaaaaatttt tgccatttaa
     1501 atataaaaat gacacttgga tttctaaggg gtcaacagag ttgggcaaca ggattttaaa
     1561 aatgatccaa atttttgaat tttaaaggag tttttttcta tggctaaaat tggatatgct
     1621 cgagtaagca gtattgatca aaatttagat agacagctag aattgttatc aaagtgtgat
     1681 aaaattttta ctgataaggc aagcgggaaa gataccaatc gtgaaggatt tcaaaaaatg
     1741 atggaattca tacgtgaagg tgatattgtc gttgtgacag aattaaaacg tcttgggcga
     1801 aataataaag agctaacaga aacgatgaat ttgattcagc tgaagaaagc gactctggaa
     1861 gttttgaatt taccagcgtt aacagggata actgatgata atttgcgtcg attacttaat
     1921 aacttaatta ttgaactata caagtaccag gcggaagaag aacgtcgata tattcgtgaa
     1981 acgcagcaac aaggtattgc gcttgctaaa gcaaaaggaa aatataaagg tgggaagcca
     2041 aagtatagag aagatgataa acgtctgcaa ttagcattta aattattttt agctggatct
     2101 acagataaag aagtagaaga gcaaacagga attaatcgaa gaacatttag aaggtacaga
     2161 atgaaatacg gaatcactag taaattgaga gagaaaaata agataaaata acttaaaaat
     2221 tcacagagat tgaaccgatt tca
//
LOCUS       DASJRQ010000194         2213 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJRQ010000194 DASJRQ010000000
VERSION     DASJRQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.202"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..1368
                     /locus_tag="U4A80_002784"
     CDS             34..1368
                     /locus_tag="U4A80_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976372.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..>2213
                     /locus_tag="U4A80_002785"
     CDS             1355..>2213
                     /locus_tag="U4A80_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976373.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLV"
BASE COUNT          738 a          332 c          384 g          759 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttg
//
LOCUS       DASJRQ010000195         2192 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJRQ010000195 DASJRQ010000000
VERSION     DASJRQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.203"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..416)
                     /locus_tag="U4A80_002786"
     CDS             complement(9..416)
                     /locus_tag="U4A80_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976374.1"
                     /translation="MTNRNEGITIYLTIGKEVKLNFDSENNLSSIRLSINKVAELFGN
                     KVVNEESIFYVSTVALVTKSNYKTFISVVGGKQVISGDAVYEEVMIDQERTSNKRGNK
                     NVYEQGYFEFFVSEEDKIPKEKLILPYNVKKRR"
     gene            complement(595..2121)
                     /locus_tag="U4A80_002787"
     CDS             complement(595..2121)
                     /locus_tag="U4A80_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1976375.1"
                     /translation="MKKKHISKVASAVLLSQMLLTTPLQVLADEQLSTPTSQSSTMNN
                     QSEESTSSSDEQTGTSTTQTATDSTESSTDQSTTDDTTTSSTDTTSTTESSEKPTEDS
                     TTDSTSTTESSSESKPDETKPSETKPSESKPEKPNTGSSTNESQTQPTTEPSNNQQAP
                     SVAPNNQAKATNEKQQAKPAENESKPYHQTPTAPIQELVPSSKETAEGNIHFEKDESV
                     ESFVRKIGESARKVGQENDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGK
                     GVSFATQEDLGDGTFYTTQATFRQYENYEDSLNDYAQLLKEGLTGNSQFYSGVWKTNA
                     KTYQEATKFLTGRYATDTKYDQKLNGLIETYHLTDYDKEVAGPQLSKEGYIVPVKNYT
                     ISSPFGTRGGEFHRGLDLAAAQGEPIYASKAGTVVKAEFHPSWGNYVAIEHEDGTTAL
                     YAHQQEYQVKVGDKVKQGQIIGYVGSTGNSTGSHLHFELCLDHSLSQSQLVDPETVLF
                     "
BASE COUNT          556 a          437 c          369 g          830 t
ORIGIN      
        1 taagcttttt acctacgttt ttttacgttg taaggcaaaa ttagtttctc tttaggaatt
       61 ttgtcttctt ctgacacgaa aaattcaaag tatccttgct catatacatt tttgtttcct
      121 cttttattgc tagttctttc ttgatcaatc attacttcct cataaactgc gtctccacta
      181 ataacttgct ttccacctac tactgaaata aaagtcttat agttactttt tgtcactaag
      241 gctactgttg aaacataaaa tatactttct tcattgacta ctttatttcc gaaaagttct
      301 gcaactttat taatggatag acgaatactt gataagttat tctctgaatc aaaatttaac
      361 ttaacttctt taccaatagt caagtagata gtgattcctt catttctatt cgtcataaaa
      421 tttgtactcc ctctttctta attatcttct ttcatcaatc atatcttcat aacgatcgat
      481 tgctactaat gacattaacg ccacaccaat aaatccgcct aaaaataaac cacttataaa
      541 tagcaacata gacaatacct ccttaaaaat tgaataaaaa aaacagcctt atttttaaaa
      601 taagactgtt tctggatcaa ccaattgtga ttgacttaaa ctatgatcta aacataattc
      661 aaaatgtaaa tggctacctg tgctatttcc tgtcgagcca acatagccaa tgatctgacc
      721 ttgttttact ttgtcgccaa ctttgacttg atattcttgt tggtgggcat ataaagccgt
      781 tgtaccgtct tcatgctcaa tagctacata attcccccat gaagggtgaa actccgcttt
      841 aacgaccgtt cctgctttgc tggcatagat tggctcgcct tgtgctgctg ccaaatctaa
      901 tcctctatga aattctcccc cacgagtacc aaacggacta gaaatcgtat agtttttcac
      961 tggtacaata tatccttctt tacttaattg tggtcctgcg acttccttgt cgtagtccgt
     1021 taaatgataa gtctcgatca aaccatttaa tttttgatcg tatttcgtat cagttgcata
     1081 tctgccagtt aagaatttgg ttgcttcttg ataagttttt gcgtttgttt tccatacacc
     1141 actataaaat tgactattac cagtcaaacc ttctttaagc agctgtgcat agtcatttaa
     1201 gctatcctca tagttttcat actgtctaaa agtagcttgt gtggtgtaaa aagtgccgtc
     1261 acctaaatcc tcttgtgtgg caaaagaaac accttttccg ttatgtgttc ctttgattcc
     1321 gaacaagtta taatttggcg cttgtgctaa ctgactttgt ccgctagctg attccaaaat
     1381 ggcttgcgca atcatgacag aagcgtataa gtcgttctcc tgtcctactt tgcgagcaga
     1441 ttcgccgatt tttcggacaa agctttctac ggactcatct ttttcaaaat gaatgtttcc
     1501 ttcagccgtt tctttgcttg aaggaactaa ttcttgaata ggcgcagttg gcgtttggtg
     1561 atacggtttt gactcgtttt ctgctggttt agcttgctgt ttttcatttg ttgcctttgc
     1621 ttgattgttt ggagcaacag acggtgcttg ctgattgttt gaaggttcag ttgttggctg
     1681 agtctgtgat tcattcgtag aactgccagt gttcggcttt tctggtttcg attcactcgg
     1741 ctttgtttcg ctaggtttgg tttcatctgg tttagactca ctactggatt ccgttgtact
     1801 tgttgaatct gtcgtactgt cttctgtagg cttttcagac gattctgttg tagacgtcgt
     1861 atctgtcgag ctagttgttg tatcatcggt tgtactttga tctgtactac tttctgtaga
     1921 atcagtcgct gtttgcgtag tagacgttcc tgtttgttca tcactacttg aagtagattc
     1981 ttcagactga ttattcatcg tgctagattg actggttggc gttgaaagct gttcatctgc
     2041 caatacttgt aacggtgttg tcaatagcat ttgagaaaga agaacagcgg aagctacttt
     2101 tgaaatatgc ttttttttca ctttttctct ttcctttcct gtattacatc taagataact
     2161 ttattttatg aattttttat aaaaatatat at
//
LOCUS       DASJRQ010000196         2189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJRQ010000196 DASJRQ010000000
VERSION     DASJRQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.204"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..>2189
                     /gene="acm"
                     /locus_tag="U4A80_002788"
     CDS             60..>2189
                     /gene="acm"
                     /locus_tag="U4A80_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1976376.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTIC"
BASE COUNT          858 a          345 c          426 g          560 t
ORIGIN      
        1 atttttgaga gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa
       61 tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac
      121 tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt
      181 tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg
      241 acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag
      301 gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg
      361 ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa
      421 acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata
      481 ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa
      541 atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca
      601 tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt
      661 atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa
      721 gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg
      781 gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata
      841 attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt
      901 ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt
      961 ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata
     1021 tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata
     1081 tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac
     1141 agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta
     1201 tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg
     1261 attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg
     1321 gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt
     1381 ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa
     1441 acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag
     1501 tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc
     1561 aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg
     1621 ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg
     1681 aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg
     1741 acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca
     1801 caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg
     1861 tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa
     1921 aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga
     1981 cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag
     2041 gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa
     2101 caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa
     2161 tcattggttt attgatagta actatctgt
//
LOCUS       DASJRQ010000197         2189 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJRQ010000197 DASJRQ010000000
VERSION     DASJRQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.205"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1168)
                     /locus_tag="U4A80_002789"
     CDS             complement(<1..1168)
                     /locus_tag="U4A80_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HEN1976377.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVF"
     gene            1189..1962
                     /locus_tag="U4A80_002790"
                     /pseudo
     CDS             1189..1962
                     /locus_tag="U4A80_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2030..>2189)
                     /locus_tag="U4A80_002791"
     CDS             complement(2030..>2189)
                     /locus_tag="U4A80_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1976378.1"
                     /translation="DGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSY
                     VNGKFCLA"
BASE COUNT          656 a          428 c          361 g          744 t
ORIGIN      
        1 cgaatacatc ttctaataaa aatgcacgca ataatctgtc aattagcata tttaccttga
       61 ttggtattac cattttacct aaatttttct cgatatatcc actgaaatga cctacgttaa
      121 cacttgattt taatttatca aatcgactat attcattaat gtttacctcg gttaagcgta
      181 aatccaacat ctcacgtaat aatgcctcat taatattggt tgaatccact aaatcttgga
      241 taaaatcgtc ctcttcacta agctgatatt cattgaggta atcagtaaat tcccatcctt
      301 ctttgataac taattcccca ttatttacat catttagaat catctcagca tatggctgta
      361 actccactgg taattgcgca aatgtgcgat gtaagtcgtt taacaatgat tgaattaaat
      421 tcgaatcacg ttcttctttt atattcatgt attttttgaa gtttgtgttt aaatactcat
      481 aattaatacg actacgacta ccttcaggta attggtaatc aatgtcatac gctatactat
      541 catttccgtc tctaggttct ctctctatct cgctgtaacg ttgtgccaag atattaaatt
      601 catattctgt gattactact gttataggtt cataatcctg gatttcataa gtcgattttg
      661 cccatgtgaa tccctgtatc atcgctgatt cataacaatg gattagttga ttataaagat
      721 ttacaaattg cttaatttcc cctggaacct ccggtaaatg actgaagttt tctatgccag
      781 aaatattaaa tacggactta atttcttcga atatatcgtt caacttttct aagttattat
      841 ataatttttc aacaaataac ccttttgctt gtttgcctga gtataactca atagcctctt
      901 caatgttctg ttccatcgta tgtgctttac gataataacg aatggttccg aacggttttt
      961 caggaccatt aagacgattt gtgcgtgaaa aagcctgaat tatgttctga tattcaagtg
     1021 acttatctaa gtatagggtg ttgacccatt ttgaatcgta acccgttaag agctgattaa
     1081 caactataac aagatctaat tgggttgctt catctactgt tttgtaagga ttcttatgcg
     1141 ctgtgttttg caatagtttt gtacacaaga ttgcaaagaa aagtacacaa tgttgccact
     1201 tctggttcca tgagttttat ttgtgttgat gggacaacca atcagctatt tacgaccctt
     1261 gaagatcgca gactttacaa actaactcaa tacttcctgc gctttcctag aaaggcccgt
     1321 ttaaaggtca agtacctagt gatggacatg aatgccagct attgtcagtt gcttaaaacg
     1381 gtctttccaa atgcggagat cgtaactgac cgctttcata ttgtccaaca catcaatcgc
     1441 agcttcaatc aattacgtgt ccagatcatg aatcgttttc gaacctcaca ctctgaaaat
     1501 cagaagaagt accgtcggct caagcgatat tggaaactct tactcaaaga ttccacgaca
     1561 tgggaggcac tcaaacggca ttatcatcga ctgtttaaac gaccaattag tcaaacagaa
     1621 atcgttaatg agctgttaag ctacaacgaa gaattgcgaa ctgcgtatca tttttgtcaa
     1681 ttattacgct attatttcgt taaacgagat acggtaggct ttcaagaaac ccttaaaaca
     1741 atttcatcta cgttacctca gtcgtttaaa aagaaattca cgatcttcca tcgctaccaa
     1801 tcgggtattt caaatgcctt taaggtgtcc cactcaaacg gcgtcactga ggggctgtat
     1861 aacaagatca agctgattaa acgaattgcg tttggttatc gcaactttta taactttcgg
     1921 gcacggatct atttacaaca aggtctgatc tttgaaaact aaaaaaatat gcggcgcaca
     1981 gaacgcgccg catatcggac taagttttat tggcttatta tttgtcaaat taagcgagac
     2041 aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa tttagtgatt
     2101 ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga ttgacttggt
     2161 tgaattccat ttcttttggt agtccatct
//
LOCUS       DASJRQ010000198         2114 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJRQ010000198 DASJRQ010000000
VERSION     DASJRQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.206"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(78..1004)
                     /gene="cysK"
                     /locus_tag="U4A80_002792"
     CDS             complement(78..1004)
                     /gene="cysK"
                     /locus_tag="U4A80_002792"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1976379.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1144..2031)
                     /locus_tag="U4A80_002793"
     CDS             complement(1144..2031)
                     /locus_tag="U4A80_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1976380.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          473 c          356 g          701 t
ORIGIN      
        1 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
       61 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
      121 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
      181 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
      241 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
      301 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
      361 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
      421 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
      481 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
      541 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
      601 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
      661 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
      721 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
      781 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
      841 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
      901 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
      961 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     1021 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     1081 tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct
     1141 aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt
     1201 tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc
     1261 acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga
     1321 tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc
     1381 tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc
     1441 cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact
     1501 tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga
     1561 acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg
     1621 ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg
     1681 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     1741 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     1801 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     1861 gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     1921 cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat
     1981 ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc
     2041 tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt aattaattaa
     2101 attatacttt taat
//
LOCUS       DASJRQ010000199         2110 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJRQ010000199 DASJRQ010000000
VERSION     DASJRQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.207"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..930
                     /locus_tag="U4A80_002794"
     CDS             <1..930
                     /locus_tag="U4A80_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976381.1"
                     /translation="KELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDE
                     IFIQGENYFTKILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKN
                     SIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIF
                     FDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEV
                     LLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNI
                     VLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            917..>2110
                     /locus_tag="U4A80_002795"
     CDS             917..>2110
                     /locus_tag="U4A80_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1976382.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFL"
BASE COUNT          717 a          267 c          331 g          795 t
ORIGIN      
        1 aaagaattag ggctatttaa tgatgttgtt tttgtgaatc accggaaaat cggttcttcc
       61 tttaaacgta aaattaactt gttaactaac cataatcaat ttttctcgga tgatttatcc
      121 gtttatgatg aaatttttat acagggggaa aattatttca caaaaatact tttctccaat
      181 gtttacaaaa aaaatccaga tgtaaaacta aattatatag aagatggttt gggtacttat
      241 ttgaagaaga gtatattttc aacaagtaaa aacgttcaaa gagtttttaa aatattaaat
      301 aaaaatagca tcttcaaaaa aaaaattgac gattattttg tctattgtcc ggaattgtta
      361 ctttatgatg taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag taaattgatg
      421 aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa atgtatcttt
      481 tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat tttcgctaaa
      541 gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc tagagcaagg
      601 acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga agtcttgcta
      661 cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat ttctttttca
      721 ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt aattttaaat
      781 gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt tcttttctgt
      841 gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg ggcagaatta
      901 agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat tagttaagtc
      961 cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca taactattcc
     1021 tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt tcacttcatt
     1081 tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta ataatgcaag
     1141 ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc tagctacact
     1201 ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa atgctttttt
     1261 tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta gttttgtaat
     1321 tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt tgattttatc
     1381 ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga cattatattt
     1441 tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt tgataggttt
     1501 agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa aacagtggag
     1561 atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata tagttttagc
     1621 acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg gaatatattc
     1681 ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta ataacgcctg
     1741 ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga agaaagttag
     1801 taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag tttcgattga
     1861 tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag tggttccgat
     1921 aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg aattttatga
     1981 gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta atatactatt
     2041 aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa ccgtatttac
     2101 gtatttttta
//
LOCUS       DASJRQ010000200         2088 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJRQ010000200 DASJRQ010000000
VERSION     DASJRQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.208"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(108..1535)
                     /locus_tag="U4A80_002796"
     CDS             complement(108..1535)
                     /locus_tag="U4A80_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1976383.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1528..>2088)
                     /locus_tag="U4A80_002797"
     CDS             complement(1528..>2088)
                     /locus_tag="U4A80_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1976384.1"
                     /translation="KHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRH
                     HKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFK
                     NKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVR
                     KVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          788 a          334 c          247 g          719 t
ORIGIN      
        1 cagaataaat aaataaatct ccttttgatt ctcggtaaaa attttgataa acatttaata
       61 tattaatcaa atgtaaagga gtccaagcaa tgaaagctgt cttcatatta attctccttt
      121 tttaaataca agtaagaaat gcttcccaaa taataccaga aaacaattga aatgacatcg
      181 cgcttattga aaagtacatg agcctccacc atttcatcta taaaaataga tagtataaaa
      241 attaaaacaa taccaagaaa cgcataattc actttacttc taaaaaatgt tttgagtaaa
      301 ctaaagatat ttaacacata gaaagcatag atacatataa aagcaattat accacttgat
      361 acaagtactt gaagaaatcc attatgcatg tttccatgtg ctctttttag ttcattcaca
      421 tctgtagtat caagatctac ttttttcaaa aattttgcat cgctattccc atagaaatcg
      481 gtctctccat aaccaaataa caaagttggt cttataactt taagcgcagc tttccaaatc
      541 gtaaaacgac cagctgaaat ttcggagtta tcactagaat gctgtaatat tacttctttt
      601 tctggaagtg gagttttatc tgattcattt gactctataa cttctacttt tctatcaatc
      661 gatacaggaa ttgttgctaa aaaatcctta gcataattag taccaaaaga aaaatatacc
      721 aatagaacta ataatatgct taccatccat ttgattttta caggtagtac ttctctaatg
      781 ctctttctaa attttgaatt ggcaaacaac aaaagaaata atactaaaaa agaacaaatt
      841 actacctggg cacctctaga cgaagaaagt gtaatataaa atatttgtac gataaaattc
      901 atcatataaa aaacacgaat cgtttttgtt atttttgaaa ttaatacact aattaaactt
      961 aaaataatag aagtatatcc aaacatagct ccaacatttg gacttgtata gacaccaaat
     1021 aaccgatgtt caataaaacc ttgtcttgca agtaatcctg gcgttcctgt tgggacatga
     1081 taagaaatca aaaaaataaa ttgatagata gatattagcg cagctaaaaa aattatgata
     1141 atgaaaacat tattaaaata caccattttt ttcttttttt cctcttcact ttgtgaaaag
     1201 tctgcaggaa aaattactat caaagtaatc agcaaatatc caaaattata aatagatgga
     1261 attaaaaaat tttctctgtt aatgattata gttaagataa aactaatcat tgctccaatc
     1321 agtagccaag aaaaacgcat tttaaaaaaa tttttcttta taaaaacctc atacaaaact
     1381 ataattccac cccaagcaaa agctattttc atcgggatat tagttacatt aaaagtacta
     1441 ggcataaaat aaagcagaca aatgattaaa tttattactc gaaacgcatc tcgacttaat
     1501 aaactcttct ctaaaacagg tactattcta ttcatctata tttccaactt tcaaaatttc
     1561 tttatcaatt ctatttaata gtttatcaga gtaatccgat ttaacttttc ttacaaataa
     1621 tttttcactt tcttttattt gatctatatc cctttcatca aaccatttgt tcatggaagg
     1681 atcaataata tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt
     1741 caattcaagt ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa
     1801 aaaaagtgta tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta
     1861 gtcttgttga ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc
     1921 ccaccattga gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt
     1981 aatcatcctc agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgttatt
     2041 cctattaatt ctaggataat catgtttttg atatacatct atatgttt
//
LOCUS       DASJRQ010000201         2048 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJRQ010000201 DASJRQ010000000
VERSION     DASJRQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.209"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            197..589
                     /locus_tag="U4A80_002798"
     CDS             197..589
                     /locus_tag="U4A80_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgR"
                     /protein_id="HEN1976385.1"
                     /translation="MELKPKNLKQITCEFVLFNRQHIKFSNRTIKRKITDFSGKEIIL
                     YAKKLSSEQELTCFFDQIKAIYFDGKIVACRGCSELPLIVSNHVFSKMKARQKLLRES
                     KVRYREMQKEEWEKRKSAARKKYKRISA"
     gene            1156..1332
                     /locus_tag="U4A80_002799"
     CDS             1156..1332
                     /locus_tag="U4A80_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976386.1"
                     /translation="MSTEKKRKKYAFSDETIEKLDWLVKEHQKRSKKRIYPCDTLSEL
                     INNEYVIRKSFGNS"
     gene            1386..1772
                     /locus_tag="U4A80_002800"
     CDS             1386..1772
                     /locus_tag="U4A80_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976387.1"
                     /translation="MNRIRFGKSNKNEFSSIFKVNVGIALVFFSLGIWGGMRVQCEQS
                     YFDNTIDEVYFEYDLATHRNYNPKESHEVSLRRVQYLTWDLYPLLKYPESVTSKLFYI
                     DKRNNEKVEKAWVTLLKLQKIALEEK"
     gene            1796..1993
                     /locus_tag="U4A80_002801"
     CDS             1796..1993
                     /locus_tag="U4A80_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976388.1"
                     /translation="MEWLKNEKELFQFLSDMKRKNQVANLDNYIKWIKDQSVQIKQDR
                     ESGNLNIKIAVTDKQNNILIY"
BASE COUNT          774 a          270 c          389 g          615 t
ORIGIN      
        1 ttaaaagaaa atttgtattt ctctggctac ttttttgagt agacttagtg tgcaattttc
       61 tagatatatt tgtttatact gtttgcagct gatttgtgac ttaagagggc aattacactt
      121 catgtgtgat tgccctcttt ttgtcgtgct tggcttccca atgatcagca actgaagcag
      181 aagggcggag aaaaatatgg aattaaaacc taaaaattta aaacaaatta cttgtgaatt
      241 tgtattgttt aatcgacaac acataaaatt ttctaatcga acgatcaagc gtaaaattac
      301 tgatttttca ggtaaagaaa ttattttata cgctaaaaaa ttatcaagtg aacaggaatt
      361 aacttgcttt tttgatcaaa taaaagctat ctattttgac ggcaagatcg ttgcttgtag
      421 aggctgttcg gagttgcctt taattgtttc aaatcatgtg ttttcaaaaa tgaaagcacg
      481 tcagaagctc ttaagagagt cgaaagttcg atatagagaa atgcaaaaag aggaatggga
      541 aaaaagaaag agtgcggcac gcaaaaaata taaacgaatc agtgcctagc tactgttttg
      601 actaatgata cttgattttt ttgtttgtta gttttttcta acaaacaacc taatagcagg
      661 gagttattgc tatcgaaaaa acggacaagt aagcacacgg acacgaaaca gcgctaaata
      721 tttatttaac gtttgctggc aaggagtcac atgacactaa gcgatacacg taaacttact
      781 ttgatgtttt gggtgttcct tgacaactga aaaattacag gactttctaa gttaatcagc
      841 gtgtctaata ggggcttagg aaggataaac cacctaaaaa agtacggctg tgttatcaga
      901 tggtacgtta ttgatgggta gggcacacac agaaaaggtt atttgcgatg atcagctatc
      961 acgaaaaaca taagatgacg attaaaaccg tccgctcgtt tttctaggga gcaaaaacac
     1021 ttttcgagag tttggggttg ctatcgctag aaaccctgcg taaaaaaact gtaacattta
     1081 tgagaaaact aataaacaaa agaatcaagt atctaaaaaa taaggggaca cctaatttaa
     1141 ctggagggta cacctatgag tactgagaaa aaaagaaaaa agtatgcatt ttctgatgaa
     1201 actatagaaa aattggattg gctagtaaaa gagcaccaaa aaagaagtaa aaaacgtatt
     1261 tatccttgtg atacactttc tgaattgatt aataatgaat atgtgataag aaagagtttt
     1321 ggcaattcat aagtaatagt ttacttcttt aattccttaa caagtatata aaggagctga
     1381 gaaatttgaa tcgtattcgc tttggtaaaa gtaataaaaa cgagttctca agtattttta
     1441 aggtaaatgt gggtatagct ctagtatttt tttctttggg aatatgggga ggtatgagag
     1501 ttcaatgcga acaaagttac ttcgataaca caattgacga agtgtatttt gaatatgatt
     1561 tagccactca tagaaattat aatcctaaag aatctcatga agtatcttta agaagagttc
     1621 aatatctgac ttgggattta tatccacttt taaaatatcc agaaagtgta actagcaaac
     1681 ttttttatat tgataaaagg aataacgaaa aagtagagaa agcgtgggtt actcttctaa
     1741 aactgcaaaa aattgctcta gaagaaaaat aaattaattt ttatgaggtg ttggtatgga
     1801 atggttaaaa aatgaaaaag agctttttca atttctaagt gatatgaaaa ggaagaacca
     1861 agtagcaaac ttggataatt atatcaaatg gatcaaagat cagtcagtgc aaataaaaca
     1921 agatagagag tctggtaact taaatatcaa aattgcagta acggataaac aaaacaacat
     1981 tttaatttat taaggagaat aaaatgatcg aagaaaaaaa gaaagtatta tattctaata
     2041 aacccgaa
//
LOCUS       DASJRQ010000202         1959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJRQ010000202 DASJRQ010000000
VERSION     DASJRQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.210"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            545..1926
                     /locus_tag="U4A80_002802"
                     /pseudo
     CDS             545..1926
                     /locus_tag="U4A80_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          728 a          328 c          344 g          559 t
ORIGIN      
        1 tatttttgag ctatgtacaa gaagcatttt attctagctt aatttgacaa atcatatagt
       61 gacaatctct gaaaactgtc cattaaatgt ttcttcctat cttgttcatt aacaaaaaat
      121 tataagccct tacaacgaat attatatatt aaatttaatt gtaggtgaac acgaatttta
      181 taaatattta tggtgttaat gacaaatgat atccgactcc tcttcatacg ttctggacta
      241 tttgctaata gattaaaatt tcagcatcta tttctgacca taaataaatg agctgccgaa
      301 aagctgccaa atcagacttg aaaataaagg aacaaaaact tttaagtttc tgaccccgta
      361 actcacactt caaaatctta cttaaaaagg agtctgaaag aaaaaaagag tattcaaaat
      421 ttttttctaa ctagctacat cacatctatg cattgacact caaagaacca gaataagtca
      481 tcagaaagca agggggtgcg gacataatcc tggacgaaaa aataaactta ggaggaatgt
      541 ccgtgtgttt tcccacaaag agcgtgtaag ggcaattcag ttatttttaa aatacgattg
      601 ctcttatgct gcaaccattc gagaattagg gtatccgtcg attggtgcac tacggaaatg
      661 gtataaagag tatttggaat ccggagagct ccatcaggag caccgaaaaa aatcaaagta
      721 ttctgaagaa caaaaacgaa tagcggtgaa tcattatttt gaatatggtc aatgttatgc
      781 acgaaccatt cgcatgcttg gttatcctaa tagagagtcc ttgcggcagt ggtgtgaaga
      841 acttgcacca ggagctagaa aactccgaaa aagtgcggta aagttaacac aagatcaaaa
      901 agaagctgtg ttgaaaaaat tctataaacc gcaaactaat aggaaaaatt tagccgaatc
      961 tgaaggaatt agtagagtta ctctatatca atggaaaaac acggttcttg gaaaggattt
     1021 tccattgcgt atgactttaa aaaatcagga aaaacaaaaa gagctacttt taaaggaagt
     1081 agaagactta caaaaacaag tacaccaatt acagctcgaa aaagcattac tcgaaggagc
     1141 ggctgaactg ctaaaaaaag aaaaaggcgt caatcttctt tgtctatcaa atcaagaaaa
     1201 aacgatattg attgatgcct tacgaaatca gttcaccttg aaagagcttc ttcaacagct
     1261 ccagttgcct aaaagcagct atttttatca aaaacaagca cttgagaaac cagataagta
     1321 ttacaaagaa cgtcaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta
     1381 ccgtagaatt caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg
     1441 tagattgatg actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc
     1501 gtatgctggg gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa
     1561 gaagccaaat gaaaaatggt taacagatat tactgaattt agaattcccg cagggaaaat
     1621 ctacttatca ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac
     1681 aaagccaact tgcttcactc tatgtctaaa aagggttatt caccagataa ttcagcgtgc
     1741 gaaggtttct ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca
     1801 acaatcaatc agtttataaa caaattggat gattatattc attggtataa taatcaacga
     1861 attaagttat ctttaggagg gctaagccct cttcaatatc ggaagaagca aagatatatt
     1921 tgttaaaaag gtccaaaaaa atatccgcac ccccttttt
//
LOCUS       DASJRQ010000203         1947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJRQ010000203 DASJRQ010000000
VERSION     DASJRQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.211"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..408)
                     /locus_tag="U4A80_002803"
     CDS             complement(58..408)
                     /locus_tag="U4A80_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976389.1"
                     /translation="MIDYNDRGRLKWTGFYLSDHTEEIDADNEQRANQNLAKEQITTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPYDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(401..1726)
                     /locus_tag="U4A80_002804"
                     /pseudo
     CDS             complement(401..1726)
                     /locus_tag="U4A80_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
BASE COUNT          541 a          405 c          309 g          692 t
ORIGIN      
        1 aatatttgta tataactata catgttatat actttagctc atattgctgg ataatgttca
       61 ttctagtcga ctccacttat tatcctggta aaattcaatg tgccgtattt cgtcgtagtg
      121 aattttgtct tttccaacgt agattcccag ctcatcacta cctaagatct ttccaacaat
      181 atcgtatgga tagtatcctt cagcatttcg ttcttctttt tgaatacata ctaatctatt
      241 ttttatgact gcagcgttca atacttccga aatctcttca gtcgttattt gttcttttgc
      301 taagttttga ttggctcgct gttcattatc tgcatcgatc tcttccgtgt gatcggataa
      361 atagaatcct gtccatttca atcgtcctcg atcgttatag tcaatcattt ttaagccccc
      421 ctgcaccgcc tgcatggcca ccaaccaacg tgcttctagt aatactacga gcgccttcta
      481 acatcgaaga agcatgtaca attgacgtaa aaccatactt ctgcctaatt ttatctatta
      541 ttttgtctag tttttcgtca tcaatctgtt tttgtggatc actaaacaag tccagctgaa
      601 gcgaatcggt gtacacaagc ttgctatagg ttattccaac gtgccttact tcttgtcctt
      661 cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta ttatctgtga
      721 cagggatctt catctggtgg aaaaatcctg gtctggtctc taagatcgac gtaccaatgt
      781 ttaaatggat acatcctgtt ttacaattat gctgtctgat acgaatggct acttgttccg
      841 ccatttcttt tatcaccagc tcgatttcat ttcttctagt ataatctcgt ggtaacactt
      901 gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gactgctatg tctgttcgat
      961 cgattccatt tgcatggaag tacagctgta agccaatcac acctaaacga ctcttaatag
     1021 tgtgtggatt ccaattagct aagtctttaa tactcatgat acccatttag ttgagccttt
     1081 ttttcattcg agagccaatg ccccaaagat ccgtcatttc aggaatattc catacttttt
     1141 cgggaacgtt ttcgtacgtc cattctgcaa caaatccttc attatgcttg gcttcattgt
     1201 ctaaggctag tttagccaaa aggggattat ctccgatacc taccgtagca atcaatccta
     1261 gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt tgacttcgtg
     1321 ttccttcagt cgtaaaaagg ttcagtgatc tggtcacttt taagatcgat tcatcgatcg
     1381 agtaaatcaa taaatcttcg tctgctacat atcttctgaa gatattgttt acctgcatgt
     1441 ttcgcttaat atagagattc attcgtggtg gaacgacatg taacgtttta ggaaatggtt
     1501 gtggtaagtc acgtggtcta ctgacattag aaataccata cttcttcttt gcagctggtg
     1561 aagaagctaa aatcaaccct gatcctgtat tatctgctag actcatgacg actaattcag
     1621 tcgttaatgg atctaaaccc ctttctatac attcgacact tgcgtaaaag cttttcatat
     1681 cgatcaaaaa ggaagtattt actggctctt ttgaataatc tatcataaat aacactccat
     1741 tcgtcacaat tatacaaaca tatgttccct tttgtaaaga aggaagttca aatgaacaaa
     1801 aaaagattcg aattgttatt acaaaaagtt taaacggata actgaccact tattagattt
     1861 taaatgcgtt agaatcttca tatatcaact aattagaata aaaaaagcaa agtaactaat
     1921 gaaaatccat tagttacttt gcttttt
//
LOCUS       DASJRQ010000204         1930 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJRQ010000204 DASJRQ010000000
VERSION     DASJRQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.212"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            233..799
                     /gene="amaP"
                     /locus_tag="U4A80_002805"
     CDS             233..799
                     /gene="amaP"
                     /locus_tag="U4A80_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1976390.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            812..1000
                     /locus_tag="U4A80_002806"
     CDS             812..1000
                     /locus_tag="U4A80_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1976391.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1013..1570
                     /locus_tag="U4A80_002807"
     CDS             1013..1570
                     /locus_tag="U4A80_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1976392.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1602..1844
                     /locus_tag="U4A80_002808"
     CDS             1602..1844
                     /locus_tag="U4A80_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1976393.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          700 a          301 c          400 g          529 t
ORIGIN      
        1 gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa gtaaatagag
       61 aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt tatgatccag
      121 caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat ctatactgtg
      181 attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga aaatgaataa
      241 ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat ttctagcgat
      301 tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc gcttctttac
      361 tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat ttgccgttct
      421 tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa ctttcgttat
      481 gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt tggtacgttc
      541 ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt caacgaaaaa
      601 tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag tagggaaaac
      661 aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa caaaagaaac
      721 ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa gagactacaa
      781 gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag catacaagct
      841 ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa cgattggatt
      901 tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg gtttttattt
      961 gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa agatggaaaa
     1021 caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag aaaaccgcat
     1081 gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg gttatgcatt
     1141 gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata tggctgaaaa
     1201 attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta agaaacaagt
     1261 agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata tttacaacca
     1321 aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg ttatcgaagt
     1381 gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc aagaaacagt
     1441 acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac gccaaacaga
     1501 taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg
     1561 tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt atttgggcat
     1621 taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa ggatcatcaa
     1681 tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga caagctttac
     1741 taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg atcggtgcaa
     1801 tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa
     1861 cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc ggttcttttt
     1921 tatatatttt
//
LOCUS       DASJRQ010000205         1890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJRQ010000205 DASJRQ010000000
VERSION     DASJRQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.214"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..721
                     /locus_tag="U4A80_002809"
     CDS             <1..721
                     /locus_tag="U4A80_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEN1976394.1"
                     /translation="LASMIYQQLFDTLVYKADNVYNGSLPIPVRCLLDEFANTGQIPD
                     FENVIATTRSRGISVDVILQNLTQISKKLYKDSWETIIGNCDSFLYLGGNEQSTHKYI
                     STQLGKETIDVVTYNESRGTTGSFTKNSQKQGRNLLDPNEVREIKGGKCIYMLRGTKP
                     FLSDRFKLERHPLFKKLKETPSGYMYQSPLDQEEFIANNDPIDFELSKNVDMKEFLKH
                     TEIETYDFEDEEIETINLDEL"
     gene            738..938
                     /locus_tag="U4A80_002810"
     CDS             738..938
                     /locus_tag="U4A80_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976395.1"
                     /translation="MGTFNAIIDILSKYAIVGGGLYAVWGAVVLGGALKDHNGPQMQS
                     GIWQIVGGALIVAAAALFKTIV"
     gene            970..1818
                     /locus_tag="U4A80_002811"
     CDS             970..1818
                     /locus_tag="U4A80_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976396.1"
                     /translation="MDGFLTWCVESILEVISFSSKTSHLTSNLENFSPQLYKYSTTIL
                     DSVSKPIAFSILALFFALELQRISNRVETNGGGQTGFEMIFKSLVRLGLCKLAMDQVS
                     LIMRAIMDITGYMTDKINHLGMGNKEVSDMLDAKKILAPILKEGLMFKVGVSTVLIIA
                     LLITIGSIVIVQVVVNMRFIELYAFLAMSPIPIATFPNDEWSSIGKNFLKSFTATALQ
                     GTLIFMVMQFYPYLLSSAFSDITNSGSASETFLMTLIGIIGNSVLLIFAVMATGRWSK
                     SITNAM"
BASE COUNT          646 a          255 c          372 g          617 t
ORIGIN      
        1 tctagcaagt atgatttacc aacaactttt tgatacgtta gtttataaag cagataatgt
       61 ttataatgga tcgttaccaa ttcctgtaag gtgtttgtta gacgagtttg ctaatactgg
      121 acaaattcca gattttgaaa atgttattgc aacaacacgt agtagaggaa tttcagttga
      181 tgttatcttg caaaatttaa cacagattag taaaaagctt tataaagaca gttgggaaac
      241 aatcatagga aactgtgatt cttttttgta tttaggagga aacgagcaaa gtactcataa
      301 gtatatttca actcaattag gaaaagaaac aattgatgta gtaacttata acgaatcaag
      361 gggaactacg ggaagtttta ctaaaaactc tcaaaagcag ggaagaaacc tacttgatcc
      421 aaatgaagtg cgtgagatta aaggcggaaa atgtatttat atgttacgtg gcacaaagcc
      481 ttttttatct gatcgtttta agttggaacg ccacccttta tttaaaaagt taaaagagac
      541 accttcaggt tatatgtatc aatcaccgtt ggatcaagaa gaatttattg caaataatga
      601 tccaattgat tttgaattat caaaaaatgt cgatatgaag gagtttttga aacatacgga
      661 aatcgaaact tatgattttg aagacgaaga aattgaaaca ataaatttag atgaattata
      721 aaaggagaga ataattaatg ggaacattta acgctattat agatattttg tccaaatacg
      781 ctattgtagg cggaggctta tatgcagtat ggggtgcagt tgttttaggt ggtgcattaa
      841 aagaccacaa tggtccacaa atgcaatcag gcatttggca aatcgttggt ggagctttaa
      901 ttgtagcagc agcggcgtta tttaagacaa ttgtttagag gatagtcttt aatgaaaagg
      961 ggatgaatga tggacggatt tttaacatgg tgtgtcgaat ctattttaga agtaatctcc
     1021 tttagtagta agacaagcca cttaactagc aacttagaaa actttagtcc tcagttatac
     1081 aaatattcaa caactattct tgattctgtc tcaaagccaa tagctttcag catattggct
     1141 ttgttttttg ctttagaatt acaaagaatt tctaatcgtg tggagactaa tggtggaggt
     1201 caaactggtt ttgaaatgat ttttaaatcc ttagtgcgac taggtctatg taaactagca
     1261 atggatcaag tatctttaat tatgcgagcg attatggata ttacaggata tatgactgat
     1321 aagattaatc atttaggtat gggaaacaag gaagtaagcg atatgctgga cgcaaaaaaa
     1381 atattagctc ccattttaaa agaaggacta atgtttaaag taggtgtatc gactgtttta
     1441 attatcgcat tactaattac tattggttcg atagtaatcg tacaggtagt tgtaaatatg
     1501 cgttttattg aactatacgc attcttagct atgtctccaa ttccaattgc gacatttcct
     1561 aatgatgaat ggtcgagtat aggtaaaaac tttctaaaat catttacagc aacagcgtta
     1621 cagggaactt taatatttat ggttatgcag ttttatccat atctcttaag ttcggctttc
     1681 agtgatataa caaatagtgg ttcagcaagt gaaacattct taatgacact gattgggata
     1741 attggtaata gtgtattact aattttcgca gtaatggcga ctggaagatg gtctaaatcc
     1801 attacaaatg caatgtagga aggtcttttc aatgaaaaaa tctcgttgta acagcactct
     1861 aattcttgga gtgctatttg tttttttgca
//
LOCUS       DASJRQ010000206         1887 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJRQ010000206 DASJRQ010000000
VERSION     DASJRQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.215"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(331..786)
                     /gene="tnpA"
                     /locus_tag="U4A80_002812"
     CDS             complement(331..786)
                     /gene="tnpA"
                     /locus_tag="U4A80_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN1976397.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1029..1887
                     /locus_tag="U4A80_002813"
                     /pseudo
     CDS             1029..1887
                     /locus_tag="U4A80_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          599 a          366 c          355 g          567 t
ORIGIN      
        1 gtaactcaaa aatacttcca aaggtgttcg ataatccagt gattttctag gaagattatt
       61 tctgcgccag cattttctaa tgtttgaatt tgtgttttgt aatcttggtt gcttgtgcta
      121 attcttgaat agccgtaaat tgccaaaatt tcatcaccct ttctggacat aagtttctga
      181 caccagttaa aatcaagtct atcagtaacc catacacccg tgaggacggg tgtattttta
      241 atccccctag aaggggtcga cactcgctgc cccttaaaag gggattttta aaagaccgcc
      301 aactgctcat cttcactcac ttacttctct ttatttttta aatggttcta cgtactctct
      361 tttacttata atatatcgaa tgcgatcatc agcttcttgt tcacggatgt atttttcatt
      421 tgtcttttga ttcaatccga ctgtactcac atagtatctc ttcgaccaaa aggatttgtt
      481 tccgtattta tatttgagat ttgcgtgctg ctcgtggatt agtaccgcac tacgaccttt
      541 cagatacccc ataaaactgg atatacatat ttttggtggt actcgaacaa gcatgtgaat
      601 atgatctagc attgcatgtg cttctatgat ttccacatct ttcatttcgc acaacttcct
      661 aagtattttt ccaagatctt ttcgtaattg cccatagatt acttttcgtt gatattttgg
      721 tgtaaaaata atatgatact tacaattcca acgagtatat gctaaacttt tatcgtcatt
      781 tgacatgaaa aaactccttt cgatctgatg agcggttggt agccactcac tagtatatcg
      841 aaaggagttt ttatcgctaa agatacttct tccacgcgta gaaacgcgcg gttttcttgt
      901 ttcgcacgct ttacgcacaa aactgactga agtcattaag aaaataaaag ggtctaaaac
      961 ttttaagttt tgtaccgacc cccaaaagtt agaccaaaaa atctaacttt tggaggtcgg
     1021 tattttttat ggctaagtat acttttgaac ttaaattaaa gatcgttcat gattacttgg
     1081 atggtaaagg cggaagtgat tatctggcaa agaaatattc cattaaggct ccatcacaag
     1141 tgaagcgctg gatcaatgcc tatcaagaat ttggtgaaga gggtctagta agaaagcgac
     1201 aaaagaaaaa atattctgtt caatttaagc tggatgcgat agagttatat ctaacaagtg
     1261 agctgtccta tcgtgaagtt gccaacacac ttaacatgaa caaccctaat ttgatcgcta
     1321 gttggatgcg tcaattccgt gagggtggga tcgatggact ctcaaaaacg aaaggatgtc
     1381 cacctatctt gtctaagaaa aatgaaccca aaaataagaa aaaatctaca actaaagcaa
     1441 cgtctaaaga acgtgaacgc atcgaggagt tggagaaacg agtccgttcc cttcaaattg
     1501 aaaatgcctt tttaaaagaa ttgaggaagc tgcgaaaaca ggaagcccaa caacgacgaa
     1561 tgaagcaatc gcacgaatca tcgcaagcct ccgaggaccg tttaaattag ttgagcttct
     1621 agaaacttta aagttcccta aggcaatctt tatgtactgg caaaaacgat tggaccgtaa
     1681 aaatccagat caagagattg aagcggaaat gcttaaagtt cgggagaagc acaaggatta
     1741 tggctgttta cggatgacca atgaactaag aaaccgcggt ttctctatca ataaaaagaa
     1801 agttcagcgc ctgatcaaga agctaggaat aaaagtaacg acatacacac gtaaatcacg
     1861 gcgctacaat tcttatcgag gacaagt
//
LOCUS       DASJRQ010000207         1854 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJRQ010000207 DASJRQ010000000
VERSION     DASJRQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.216"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="U4A80_002814"
     CDS             complement(140..1288)
                     /locus_tag="U4A80_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976398.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4A80_002815"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4A80_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN1976399.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DASJRQ010000208         1848 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJRQ010000208 DASJRQ010000000
VERSION     DASJRQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.217"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..397
                     /locus_tag="U4A80_002816"
     CDS             200..397
                     /locus_tag="U4A80_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976400.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            503..985
                     /locus_tag="U4A80_002817"
     CDS             503..985
                     /locus_tag="U4A80_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976401.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1043..1639)
                     /locus_tag="U4A80_002818"
     CDS             complement(1043..1639)
                     /locus_tag="U4A80_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1976402.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          573 a          275 c          369 g          631 t
ORIGIN      
        1 gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca acagctgttt
       61 taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt taactgtaaa
      121 taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag aaaagttcca
      181 gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac aatatgcatg
      241 caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa tgacaaactg
      301 cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag atgaaaaagc
      361 aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg acgtgcggcg
      421 tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat tttctggaga
      481 agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt ttttattcgt
      541 tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc ggactgtgcg
      601 aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt ggtatacact
      661 catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa tgatttttat
      721 tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga atagctttga
      781 caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc caaaaggttc
      841 taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc tgaaattttc
      901 tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg aggcagagat
      961 tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt gtcacaacct
     1021 ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca tcgttcatgt
     1081 attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa cgtagtttcc
     1141 agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg ttgtaatgtt
     1201 gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct gttgcgattc
     1261 cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat tgttcttgta
     1321 gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca tttttgaaac
     1381 cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg cgaagtgctt
     1441 cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact gcgcgtgttg
     1501 attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa gttgttaatt
     1561 cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg tttttcaaag
     1621 tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc atttttttaa
     1681 ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata tgctcacaaa
     1741 aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa ggattttctt
     1801 agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaa
//
LOCUS       DASJRQ010000209         1823 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJRQ010000209 DASJRQ010000000
VERSION     DASJRQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.218"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1333
                     /locus_tag="U4A80_002819"
     CDS             128..1333
                     /locus_tag="U4A80_002819"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1976403.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LHTGSVAKF"
     gene            1387..>1823
                     /locus_tag="U4A80_002820"
     CDS             1387..>1823
                     /locus_tag="U4A80_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1976404.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          593 a          340 c          340 g          550 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaatcgt
      301 taaaaatggc aagaagatct ctatgattct cctgaatcgt agcggtaata aacggaccta
      361 tcttcggttg aagaaacagc gatacctctg ccgagcatgt aaaaaatatt ttaccgctcg
      421 gacgtatctc gttaccccat tctgctttat ttctaagcag attcattaca agatcttaga
      481 agaattgaca gagcgtcaat ctataaaagc aattggtaaa cattgcgatg tttctgtgac
      541 aactgttcag cggacattgt ccgccttgtc atcagatatc cgtcagaatc ttgagtattt
      601 acctcggtgt ctattattcg atgagtttcg ttctctttct acatggcacg gtaaattttc
      661 tttttcttgt atggacggtg agaccgggaa actgtttgag atcttaccta gccgccgtaa
      721 aaaagatctc gttgcttttt tcatgcgttt ttctctcaaa gcgaggttgg gtgtacatta
      781 catcgtaacg gatatgaacg ctccttattt ctcactagtt aaagagtgtt ttccaaatgc
      841 acaagtgatc gtcgatcggt ttcacattgt tcaacatctg aaccgcttct tcgatgcggt
      901 tcgaaaacgt gtgatgaaaa cacttgatca aaaagaccca atacaagcta aacagtatcg
      961 ccaattgaaa tccttattca aattactttt aaaatcagag gatcgtttag attacaatac
     1021 actaacgaaa agacgcaatt tcaattggag actattgact gaaacagaag tagttgaccg
     1081 ccttctaaag atttctcctg aacttaaaac agcttatcgt tactaccaag acctgcttac
     1141 tgcgtatcag gaaaaagatc cagatacttt tttccagctg cttcgagcaa tgcctggaga
     1201 agttccattg gaactgcacc atatcaaaaa agcctttttc aaatacgaga aagggattcg
     1261 gctggcatta ctgaagaagt attcgaatgc acgactggaa aatctccata cgggttctgt
     1321 tgcaaagttt taaatctact atcaaataag gtagaataat agaaaaagat agcaggagga
     1381 atgacgatga atcattttaa aggaaagcaa tttcagcagg atgtgattat tgtagccgtg
     1441 ggctactatc ttcgttataa ccttagctat cgtgaagttc aagaaatctt atatgatcgt
     1501 ggcattaacg tttctcatac gacgatttat cgttgggtgc aagaatatgg caaactactc
     1561 tatcaaattt ggaaaaagaa aaataaaaaa tccttttatt catggaaaat ggatgaaacg
     1621 tacatcaaaa ttaaaggaaa atggcattat ttgtatcgag ccatcgatgc agatggttta
     1681 accttggata tttggttacg taaaaaacgg gacacacaag cagcctatgc ttttcttaag
     1741 cggttagtga agcagtttga tgaaccgaag gttgtagtca cagataaagc cccctctatt
     1801 acaagtgcct ttaagaaact aaa
//
LOCUS       DASJRQ010000210         1802 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJRQ010000210 DASJRQ010000000
VERSION     DASJRQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.219"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(176..1195)
                     /locus_tag="U4A80_002821"
     CDS             complement(176..1195)
                     /locus_tag="U4A80_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1976405.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1192..1416)
                     /locus_tag="U4A80_002822"
     CDS             complement(1192..1416)
                     /locus_tag="U4A80_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1976406.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1413..1706)
                     /locus_tag="U4A80_002823"
     CDS             complement(1413..1706)
                     /locus_tag="U4A80_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976407.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          512 a          368 c          306 g          616 t
ORIGIN      
        1 ataaagccat aaacaatcaa ccagtagaat gtgtggcaac aaacctgtta tcgcatagct
       61 tggagtgtga ctattatggg tgatagtgaa gatatgcgat aacatcacta ttttatcgaa
      121 tgatttttat agttgtcaat atagttatgt aatgctcctc aacgaggagc tatttttatc
      181 gtttaggaat atttaaatac caacgtttgt catggaaatc ttgcgcaccg cctttagtgt
      241 ttccctctgg atcattcgtt gcccgcatca tgacatagac tttcttatta gggaagttac
      301 gcatgttaaa agatacatga tagccaacat ttccagaagt attataagct tgatttacat
      361 ctgatctata aattccatca gctcttactc gagctaattc tttcccagta ttgtaatcca
      421 taatgaagat atactcgtat ttatagttag caatgtgcca tccagctaca tgcaaatttg
      481 cattttcgat ttctccaaac tgatcaatgt gggcgtaatt cgttccatct gtcagtgtag
      541 gattagctgc accagctcgt gttggatcaa tgacaggctt gttttcagaa gttgttggat
      601 tttcatcggt aaatccatga gctaaatcat atgctaattt ttctttactt acgcccattt
      661 cagaaagata accgtaagga tctgtatgat cgccccagat gttttgtgtt acccataaat
      721 gcgatttgat tcccggttgg ttataaggag tgtccaacgt taatggaata ccatatttca
      781 ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt tttctcaaac gttgctttat
      841 catgtgtgtg ttgtaactca atctgcacag gactgttggc attagcatac gaaccagcac
      901 cgtactgtac ataaccaggt tgaccgactt gataaacaat tccgccgtct cccacaatat
      961 aagcagtata agcgctagtc catgaacgtt gcatatactg cgcttcattg cgtcctgttg
     1021 ctgtttcatt agccgtttca tgcagtaaaa tatactgatt atttgctact tgtgagctac
     1081 cttcatttgc gcccaaatta aattcattgt tgatagtata ggcaaatccg ttaattggca
     1141 ataaaaaaag agccgttaac aggctcatcg cagtaatagt aattttcttt ttcatttgtt
     1201 tcctccttct tcgctttcag ccgagaacat tttgtaggtt cgatttgata cacccaacac
     1261 actccctaaa aacgcgccaa aaccagtaat gatgacaaca cagatatctg tgtactgcca
     1321 attgagcgct ttaccaacta accccacgaa agtagctagt gcgggaataa ttaccagtgc
     1381 gaaccatttt agtacttcga acgttttatt attcattttc ttctctccct aaacaaagtt
     1441 ttgatttgtt gcgtgtgttc caccaatttt tctgtatgtg tatctaatct ttcatcgtgt
     1501 ttctttagtt cttcatgaat catcaatcga tctgatttgc tcgattctaa atctttagtc
     1561 agcaaatcta aattgtggct tacttttgaa agagtctcag taattttcga gaaagatgca
     1621 gcaatcggtc taattactaa taaaatcaaa gaaacgatag cggttattga tcctgctatc
     1681 attccccatt cccctaaatt aatcatgtga caactccttt accttaaata aaaacgcatc
     1741 aattaagatg cgctcttatc tttattaatg attttatctg cttcttcgtc tgtaatgcat
     1801 aa
//
LOCUS       DASJRQ010000211         1779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJRQ010000211 DASJRQ010000000
VERSION     DASJRQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.220"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            102..482
                     /locus_tag="U4A80_002824"
     CDS             102..482
                     /locus_tag="U4A80_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1976408.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            464..1378
                     /locus_tag="U4A80_002825"
     CDS             464..1378
                     /locus_tag="U4A80_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1976409.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1338..1616
                     /locus_tag="U4A80_002826"
     CDS             1338..1616
                     /locus_tag="U4A80_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976410.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
BASE COUNT          661 a          363 c          343 g          412 t
ORIGIN      
        1 gccctttagt aagctttagt gagctttact tattgtacct ttttaggtac aatggtattg
       61 cgaacgatac gtccgtagga cgtactgaaa gggtgggact tatgagcgaa caaaatcaat
      121 atttggctag caatcattct aaaaaatata cctatcgatc cgagccaaaa caaatcagtt
      181 ttcgagtgag cgaatccgaa tttgcaaagc tgaagcagtc agctgaagct ttgcaaatga
      241 gtgtgccggc tttcgtgaaa gccaaggcac aaaacgcgcg tctggtgaca ccgaaagtgg
      301 cgccagacat tgctcaggca atcgcgcgcg atttagccaa agctggcggg aatatcaatc
      361 aaattgccaa gtggtgcaac acacaccaac acgacgttga tcctggcgac gcccaacgct
      421 tatccgaaaa cctaaaaatc atgcaaaagg agctacaaaa gatatggcaa cagttaaagt
      481 aagccgaacc acttcttgta gccgagcgat caattacgcc gaaccacgag caaccgtcaa
      541 aacagggata aactgtgaca ttgattatgc caaaagcgaa atgaaacaaa ttcgcatgct
      601 gtatggaaaa gacgatcgtg tacaagcaca cttattgatc cagtcttttc gaccaggaga
      661 aattaccgct gaaaaagcca atcaactagg ggcagagtat gcgaaaaaaa tagcccctga
      721 ccaccaaatc gcgatttaca cacatacaga caaagaccat attcataacc acattgtcat
      781 taactccgtc aatctggaaa ctgggaacaa atttcaagcc catggtcaag catttttaaa
      841 caaatgttat gacctaaatg acgagatttg tcttgaacat gacttaacaa tcactgaacg
      901 agggaagaaa cccgaaaaac gaaccatgtc tgaaatcaaa ttaaaagaaa aaaatgaacc
      961 tgtttggaaa gacgaaattc ggtttgcgat tgatcaaaca atgaaaaaca aaaaaactcg
     1021 aacgtataac caattttgtg attctcttaa aacattcggc attcattgct tgaatcgtgg
     1081 gaaaaatttt acttacgaat tgatccacaa aaagaaaaaa gtacgatcca acaaactagg
     1141 aaaggattac gaaaaggaga cgattttaca tgagctggac agacgagaac gagcctacaa
     1201 tcaacaacga accaacgagc tacgaagagc tcgagctagc attcgcgaaa ctgcaaaaga
     1261 agtacgaaga gaacaacgaa ctcttacgac tgaacaaccg gaaattagac cgactcgaag
     1321 aaaccatgac caacacgttg aaagaggagc agactaccaa caagaattat cgttctaaaa
     1381 cgttacaaga catagaaaat gaaataaaga aacaacgtgc ttttcttgca ccttatacca
     1441 aagaaagaat acaaactaca attgattcac aagtcaaaaa attccaccat gcgctattaa
     1501 accagcgaaa taaagaacag aaaaaagtaa ttatgcagcg atcaatggat attcttgccc
     1561 ccttatgttg cttaatcatg acactgttag ccttttggct ggtatgggga gaataaatag
     1621 aaatggcatt ctatttaccc tatctactca tttttgccag tattttagga attatttggc
     1681 ttagctataa ggcttatcaa acaagatatt cattaaaagg ctctaaaatt cgatttaaga
     1741 ggtttttatt gccaggggtt atatttacat tatcaatcg
//
LOCUS       DASJRQ010000212         1720 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJRQ010000212 DASJRQ010000000
VERSION     DASJRQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.221"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..655
                     /locus_tag="U4A80_002827"
     CDS             <1..655
                     /locus_tag="U4A80_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1976411.1"
                     /translation="LKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQN
                     HDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFE
                     CPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCR
                     EILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            670..1422
                     /locus_tag="U4A80_002828"
     CDS             670..1422
                     /locus_tag="U4A80_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1976412.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
BASE COUNT          670 a          202 c          293 g          555 t
ORIGIN      
        1 attgaaaaga aaagaattgg tttatctaaa aaaatatact gattcactgg aaaaagatca
       61 agaaaaattt gccagattta gacatgacta taaaaattta ttgctgagtt taaaagaaat
      121 cgctaatcaa aaccatgatt cgatattgga aaaagaaata aaagaattag agatctattc
      181 taattcttat ctaaccaata agtttgaata tcgttattta aaaaatataa aaaatgaata
      241 tttgaaaagt ttgttgatag cgaagctata ccaagcaaat aacaataaga tttgttgtaa
      301 attcgaatgt ccaagtacta ttattgaagt tcctgttcca atatttgact gtattagggt
      361 gcttggaatt attctcgata atgcaattga agcatctata gagagtcaag atagagaact
      421 gtctctagct atttaccgag atgataaaca gatagaaatt tcgatatcga atagttgtag
      481 agagatacta ctctctatag atactctaat gaaaaaagga ataacaacaa aaaagggaca
      541 tcaagggtta ggtttatcta gtatagagga aatcaataaa aagaatccca atatgtttgt
      601 gaactatcaa aacgatccgt atcaatttac tactcaagtt attttgttgt tttgagggaa
      661 ggaggattta tgagttatcc aataatatta tgtgaagatc aaattataca attaaatcag
      721 ttagaacgaa ttattgataa tttcattctt tttcacgata aagtatttaa gatagtattg
      781 aaaacacaaa gtccgttaga agttaaaaaa tacctcaaac aattccgacc aaagcaagga
      841 atatatttct tagatattga tttaaatcat gaagttaacg gtatagaatt agcagaagta
      901 atcagaaaat atgatgttca agcaaaaatc atttttacaa ctactcatga tgagatgtta
      961 cccgtaacaa taaaaagaag agttgaaacg ttaggatttg taacaaaaga tcaaacacta
     1021 gatgagtatc gaaacgagat tgttgagtta ttgttattag cgcaagaaag gatagatgca
     1081 acaaaacagg ttacgaatca agcatttgtt ttttccatag gttctcaaac atttactttt
     1141 gatataaatg acatttattt tgtagaagct tcaaaattac cacatagatt atcattgtgt
     1201 actaaagatg ggcagtacga attttatgga agaattagtg agcttgaaaa gaaatatcct
     1261 atgttaacaa gaattagtcg agcgtgttta gtaaatatct ttaacgttaa agaaatagat
     1321 tttaaaaaac gttcactata ttttgattca gagttggcca gaaactttac tttagggaaa
     1381 gcacaaaaaa ttaaggagaa gttgaaagaa agcactgttt agtggttttt tctttaggga
     1441 gattgcattt ttctaataaa atagattcct tcactagtgc aaatttttct gaatcctatt
     1501 aaagttgtac caatttttat agctgttatt agttacgtgc ataacagcaa tattagttac
     1561 tggaggaata tttctatggt ctggaaccaa aaaagggcca aatgaatagt tcttttcttg
     1621 tttttattaa gtaccataaa ggggaattaa aattaaatgt atggtggaag atgcgaaaga
     1681 tttggcaaat tctactaatt tttagaaagt gtttttcatt
//
LOCUS       DASJRQ010000213         1705 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJRQ010000213 DASJRQ010000000
VERSION     DASJRQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.222"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..1636
                     /locus_tag="U4A80_002829"
     CDS             158..1636
                     /locus_tag="U4A80_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1976413.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          623 a          233 c          305 g          544 t
ORIGIN      
        1 tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa tgagaataac tgatgaaaaa
       61 acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc
      121 aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga
      181 agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta
      241 tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta
      301 tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc
      361 agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag
      421 aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt
      481 aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag
      541 aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt
      601 tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg
      661 tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt
      721 tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac
      781 cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga
      841 tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt
      901 tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat
      961 cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga
     1021 tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat
     1081 tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca
     1141 tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca
     1201 gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca
     1261 agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga
     1321 ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt
     1381 ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg
     1441 atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt
     1501 atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac
     1561 tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac
     1621 tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt
     1681 tatgtttttt tcaaagaaaa ggtaa
//
LOCUS       DASJRQ010000214         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJRQ010000214 DASJRQ010000000
VERSION     DASJRQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.223"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4A80_002830"
     CDS             131..505
                     /locus_tag="U4A80_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1976414.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4A80_002831"
     CDS             complement(619..1248)
                     /locus_tag="U4A80_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1976415.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4A80_002832"
     tRNA            complement(1382..1454)
                     /locus_tag="U4A80_002832"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJRQ010000215         1654 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJRQ010000215 DASJRQ010000000
VERSION     DASJRQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.224"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1144
                     /locus_tag="U4A80_002833"
     CDS             <1..1144
                     /locus_tag="U4A80_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1976416.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1425..1654
                     /locus_tag="U4A80_002834"
                     /pseudo
     CDS             1425..1654
                     /locus_tag="U4A80_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          414 t
ORIGIN      
        1 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
       61 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
      121 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
      181 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
      241 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
      301 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
      361 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
      421 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
      481 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
      541 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
      601 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
      661 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
      721 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
      781 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
      841 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
      901 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
      961 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
     1021 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
     1081 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
     1141 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
     1201 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
     1261 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
     1321 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
     1381 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
     1441 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
     1501 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1561 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
     1621 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DASJRQ010000216         1618 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJRQ010000216 DASJRQ010000000
VERSION     DASJRQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.225"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            345..944
                     /locus_tag="U4A80_002835"
     CDS             345..944
                     /locus_tag="U4A80_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976417.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            993..>1618
                     /locus_tag="U4A80_002836"
     CDS             993..>1618
                     /locus_tag="U4A80_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEN1976418.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNL"
BASE COUNT          559 a          265 c          334 g          460 t
ORIGIN      
        1 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
       61 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaatttt
      121 ttaaaggggc aatattaatg ttctgagata atcaaatctt accgaaaata tcaaaagtcc
      181 aatgtgacac aattggattt tgtcacattc gttaaaatcg ctccatcctt agaggctcta
      241 ataggaaatc acttttcttt tagcgccttt tttctatagc ttgtttaaat gtgacttatt
      301 atctgttata atcgtattaa gtaacatttg agaggtgtct aaaaatgaaa agcgtggaac
      361 cgattcgcga caaaaaaaag atcgatgcca tgaaggccat tttggcttcc ggaaaatatg
      421 ggcaacgaaa tttggtgctt ttttcgatcg ggatcaatac agcctatcga atctccgatt
      481 taagacagtt aaaattaagg gatgtgctag aaatttcccg tgggcgggtg atcgtcaaag
      541 aacgtctggc catgaaggaa caaaaaacgg ccaaacacaa ttcggtcttc atttcgaaca
      601 aattgcgaaa agtgattctt gattatgttc aatccgaatt tcctgagcag ttgcaagcac
      661 aagacttcag caagtatttg tttccaagtc gaaaaggagc agatacgcct ttgacccgac
      721 aaagcctttg gcggatcatt catgaagcag gaactgcagt tgggttaaaa gaaattggtc
      781 cccattcgat gcgtaagacc tttggctatt ttttatacaa gcaaggaacc aaaacagaga
      841 tcattcagtc gctgctaaac cattcttcac aacgggagac cttacgctat attggaatca
      901 cccaggaaga caaagatact gcagtaaaga gtttggattt atgacagaat aatgccctat
      961 ataatagaag gaaaaattca agaggtgaga aaatgaacga agaaaagcag cctaactttc
     1021 ctgataaata tcacttatct cgcaaagagt ctgtgtattt attgaagaaa aacattgtag
     1081 aactggtgta taatgcgggg aaatttgaag ggttaaatac aacgttgtta caaacagaag
     1141 ggattattaa gtataatcgg gccaacaacg tggctgtaga tgatgtcctg accgtcgtta
     1201 acttaaaaag aggctttgaa atgcttttaa atgacgtaca ggagcccctg ttagagacaa
     1261 gtaagcggat taatcggatc gttgcagcag aggatgcgct ttttcccggt gaaatacgaa
     1321 ccggtggggt agaattatcg acgattcaag gaagatatgt gccaccaatg ttaactgaag
     1381 acgaggtaaa caatcaatat ggtgagataa tgaatcaaga gatatcagat acggaaaaag
     1441 ccctgcgact gtttctattc atttcgaaaa atcaaatatt ctggaatggt aatgtgggta
     1501 cagccttgtt gattgcgaat aagattatgt ttagcaacgg tttaggacta ttatcaatac
     1561 ccgaaaatgt atttatgaaa tttaacgaac ttttatcaaa gtattgcaac agtaatct
//
LOCUS       DASJRQ010000217         1571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJRQ010000217 DASJRQ010000000
VERSION     DASJRQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.226"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..917
                     /locus_tag="U4A80_002837"
     CDS             9..917
                     /locus_tag="U4A80_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN1976419.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            917..1537
                     /locus_tag="U4A80_002838"
     CDS             917..1537
                     /locus_tag="U4A80_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976420.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          562 a          224 c          358 g          427 t
ORIGIN      
        1 gtgatttaat ggcaaagtgg aatgtcatat taagtacaac agaaccctat aattacgtgg
       61 ggatgattca agttcgacaa ggcaataaga atacagaggt tatggaagcg actatagttg
      121 aaaatggtct tccctacgat ttatcagaat gtaaggtata ttttgaatca gttgtaggtg
      181 ggaaatatcc agtccaatta gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact
      241 atatttttga taaatattcc atgcagtgtt tacatcgaca aacagccaat ttcattatat
      301 ttaaaggaga agacttgatt ggaacaactc aagacttctc ttattttgtc attaatgctg
      361 tttcaaaaac agaaggagaa atgggttctt attggcaatc aatcgaagat ttaattgcgg
      421 atatgacgga ctttattaat gaaaataaag gcgattttac ggactggatg aatgaaagga
      481 aagaagaatt tgaacgttgg agacaagaac aggaacacag tttccaagat tggagagaag
      541 gacaagaatc agactatttg gcatggtttg aatcaatcaa ggatatttta aagacgattg
      601 atccaggtgg agtcatgtta gccgaactaa cggatgcgcg agtagatatt caaggagttc
      661 gtcatgcgtc tatttcagag cgtttgctgg cagatatgga ctatttatat cagaaattgc
      721 gagcaacgct tttcacgatt gaatacgggg agattgaagt gactgatatt ttgcaggatg
      781 atctcttttc agataatcat gaagtcgaaa aagttggaac tgtagcattc ccgattgaag
      841 aaggagcctt gatcatcgca accgttgatg atccaaagca aaatgtgttc actcttgaga
      901 aagttggggt gatctaatgg ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg
      961 tgtacaacgc caattctttc caatcacaca tgcttccgca gttcttgggt tagaagaaat
     1021 aatagcagga gaagcaacag ttttatctgt taatggaaaa attggagccg tcgtcattac
     1081 taaagaagat ttgggattag agaatgttct cacagaatta ccctatgcaa gtgaagaaga
     1141 tgacggcatt atcacggcag aaatgtatca aaaaatttta aacagtggag aaggtgacta
     1201 cgtgttgcca gttgccacta tcgaccgttt gggtggcata aaagttggtg aattattgac
     1261 gattgatgag acagggaaag tctctgcagt cagacaatct gatgtcaatt tttcgctgga
     1321 gttaaaagaa aaactcgatt cactgaaaaa ttatactgct ggagaaaaca tcactattga
     1381 tgaagatgga acaattagtt ctacaggtgg tagtgggact ggtggtgtca atcagtcata
     1441 tgtagatcaa aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat
     1501 tccaaatttt acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat
     1561 aaaacaaagc a
//
LOCUS       DASJRQ010000218         1548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJRQ010000218 DASJRQ010000000
VERSION     DASJRQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.227"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..359
                     /locus_tag="U4A80_002839"
     CDS             <1..359
                     /locus_tag="U4A80_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1976421.1"
                     /translation="NLDKDVYFSVVSARRNPYFNMVKRIENEVTIVNKSRYTSRQQTP
                     EVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFL
                     EDSSELQDIYNYLYRR"
     gene            364..1311
                     /locus_tag="U4A80_002840"
     CDS             364..1311
                     /locus_tag="U4A80_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1976422.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          519 a          215 c          314 g          500 t
ORIGIN      
        1 aaaatttaga taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta
       61 atatggtaaa aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta
      121 ggcaacagac acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat
      181 ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt
      241 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
      301 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
      361 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
      421 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
      481 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
      541 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
      601 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
      661 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
      721 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
      781 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
      841 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
      901 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
      961 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
     1021 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
     1081 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
     1141 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
     1201 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     1261 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     1321 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     1381 atcaaatctg ggatagaagg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa
     1441 taatttggac agaacagaaa gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat
     1501 gttttgccat gacgaatcat aattcgtagt ttgaaattgt aaaagttg
//
LOCUS       DASJRQ010000219         1536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJRQ010000219 DASJRQ010000000
VERSION     DASJRQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.228"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="U4A80_002841"
     rRNA            complement(<1..>1536)
                     /locus_tag="U4A80_002841"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DASJRQ010000220         1533 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJRQ010000220 DASJRQ010000000
VERSION     DASJRQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.229"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            817..1290
                     /locus_tag="U4A80_002842"
     CDS             817..1290
                     /locus_tag="U4A80_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN1976423.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1299..1526
                     /locus_tag="U4A80_002843"
     CDS             1299..1526
                     /locus_tag="U4A80_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976424.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          510 a          250 c          235 g          538 t
ORIGIN      
        1 cctatgatga aaagattgta ctattcattg atcataacga ttggctattt gattgtatca
       61 aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac tacattatgg
      121 gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa aaaataaaca
      181 tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat tcggcaatcc
      241 attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa actaatccaa
      301 tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca tgcgtcaatg
      361 cttgattttc cttttgtata acattgtata agcaataaag gacaaataag tggtgattac
      421 accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt gaaaaaatta
      481 atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt taacttacac
      541 ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa tcccaggcat
      601 ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt ttaaatgttt
      661 cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat cattttttat
      721 aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata attattctgc
      781 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
      841 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      901 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      961 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
     1021 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
     1081 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
     1141 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
     1201 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
     1261 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
     1321 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
     1381 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
     1441 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
     1501 actcgaatac tataccagaa gaataaaata aag
//
LOCUS       DASJRQ010000221         1532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJRQ010000221 DASJRQ010000000
VERSION     DASJRQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.230"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1488
                     /locus_tag="U4A80_002844"
     CDS             22..1488
                     /locus_tag="U4A80_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1976425.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          496 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DASJRQ010000222         1458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJRQ010000222 DASJRQ010000000
VERSION     DASJRQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.231"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..1049
                     /locus_tag="U4A80_002845"
     CDS             9..1049
                     /locus_tag="U4A80_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1976426.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1298..>1458
                     /locus_tag="U4A80_002846"
     CDS             1298..>1458
                     /locus_tag="U4A80_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1976427.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          521 a          188 c          311 g          438 t
ORIGIN      
        1 attaataaat gaggagaaaa gaagtgaaag ttatatcatt cggagaatta ttattgagac
       61 ttgcagcacc cggttattca aagttatttc aaagagatag tttggagact acattctgcg
      121 gaggggaagc taatgtagcg gtatccttag caatatttgg atatcaatca gaatttataa
      181 caaagcttcc ggattcagat gttggatatg cggcagctaa tgctttgcgt tattttggag
      241 taaatgttga taatattata tttggagagg gacgcatggg actgtattat ttagaaaaag
      301 gagcagctca gagaccatct aaagtaattt atgacagaaa attttctgca atttcgttag
      361 ctgatagtga tgaatttaag tggaaggaga tcctaaacga tgcaaattgg tttcattgga
      421 cagggataaa tccagcattg tctgagaatc tctttgagac gtccttagat gcatgtcagg
      481 ctgccaaaaa aagaggtata acaatttctt gtgatttaaa ttatcgatcc aagctttgga
      541 catcagagga agcaaaagaa aagatggaac agatactacc attggttgat atttgtattt
      601 gtaatgaaga agatgcagaa aaagtgctag gaattaaagc tgagcataca aatattaata
      661 gtggagaatt aaataaagat ggctatgtgc aggtggctag ggagattcac aagaagtttg
      721 gatgtaggta tgtagctaca actctgcgta gaagttattc ggcaagtaga aatggttgga
      781 gtgcaatgtt atacgaagca gaaaaagata aagcgtattt ttccacagaa tacaacatac
      841 aaattgttga tcgcgttggt ggtggagaca gcttttctgc tggtataata tatgcgttat
      901 gtgaaaatat gtccggacaa aaggctattg aatttgcgac tgcatctagt tgtttaaaac
      961 atactataga aggtgatttt aatcgaacta caattgagga tgtatataat ttaatgaata
     1021 gcggtggaaa tggtcgcgtg ttgcgttagt caacatataa agataaatca cagtaatttt
     1081 tatggtgatt atctgtctta gtagtgatta gaataacgtg aaaataaatt gttgagcgtt
     1141 tatactgttt tgaaaaataa atttaacata ttgaaaaata caaatatata cgtatattat
     1201 aaagcgccga ttgtaaaatt aagctagaca actaaaacag tcatttgtgc tacactcaaa
     1261 atagttccga ccaaagaatt ataaggagtg agtgcaaata acctatacct atcttacaac
     1321 ggatgaactt gtaataatag agtcttattt gaaaattaac caatctgttg ctaaaactgc
     1381 gcattgcttg aatcgttcaa gtcaaacgat ccataaagta tatctgttct tcaagcaaag
     1441 aaaatcagcc ttagaata
//
LOCUS       DASJRQ010000223         1433 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJRQ010000223 DASJRQ010000000
VERSION     DASJRQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.232"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..786
                     /locus_tag="U4A80_002847"
     CDS             172..786
                     /locus_tag="U4A80_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976428.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(810..1160)
                     /locus_tag="U4A80_002848"
     CDS             complement(810..1160)
                     /locus_tag="U4A80_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1976429.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1154..1417)
                     /locus_tag="U4A80_002849"
     CDS             complement(1154..1417)
                     /locus_tag="U4A80_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN1976430.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          456 a          270 c          231 g          476 t
ORIGIN      
        1 aaaaaatatt gccatttaaa aagaaaagtg acacatacta tttcctccat cattagtttt
       61 tgtctatata aggtataccc taactagaca cgaccgcgac attgaaaaat ctggttttct
      121 cgtgtatgat tagggtgttt tttaaatagt tgcagaaagt aggtaaaaat tttgagagtt
      181 gcatacatac gtgtctcatc catcgatcaa aatgaacaac ggcagattga agaaatgaaa
      241 aaattcggtg cagagagaat tttcattgag aaacaatctg gcgcaacgat tactcatcga
      301 cctgtttttc aagaagcact ggatttcgtc cgagatcaag atatttttat tgtggaagct
      361 attgatcgac ttggaagaaa ttacgacgag atcatcgctt cagtcaatta cttgaagaag
      421 aaaaatgtgc agctgatcat cacaagtcta ccgatcatgg ccgaagcaat tggcaatcct
      481 ctcctggata aattcattaa agatttgatc attcaaattt tggccatgat cgctgaacaa
      541 gaacgtaccg aatctaaacg acgtcaggca caaggcatta agattgcaaa agcgaacggt
      601 gtttataaag gacgtcctaa gctatacagt gccaatgcaa aagatcccca acgtcgtctt
      661 gtttataaaa acatcgttga ggatctaaaa aaaggtgttg cgatcgctaa gattgcaaaa
      721 gactacaatg ttacgagaca aaccgtatat agaataaaaa aagacagcat ggtcaatcac
      781 gaatagttga tcatgctgct gtttctattt tatttgctta ctttttgaaa aactgttcta
      841 actttcatca ataactcctc aactaaacta tcatgcgctt tttccaaaaa aatacactct
      901 ctggcttcat aatcaattgt cgttagttga tctaataata cttttcctgt tgtcttcatt
      961 tcatattctg agtttatatt tacatatagc ggatagtttc tagttgtact agaaattgga
     1021 gcaactgtaa caacgttact ataatccgca ataatatcat gacttaaaac aatataaggt
     1081 cttttacctg tctgttcgtg tccagatcga ggtgctgtat tcaataatag aatatcgcct
     1141 tgtctaggtt ttctcaccat ttttcattac ctactgcttc acctaaattt tgaatcgtca
     1201 catgatttct ttcatcagtg taattttcaa ataactgttc aattgttaat tcattttcta
     1261 tattagccgc ttcgataatt agccgcttct gaccgtttac ttcttcttgt attatcttca
     1321 ctttgtcttc tgtatgaatt ccaagatatt ccataatata ctttggcaat ctaaggccat
     1381 tactatttcc ccattttttt ataggtaaca tttccatagt agacacctct ttc
//
LOCUS       DASJRQ010000224         1427 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJRQ010000224 DASJRQ010000000
VERSION     DASJRQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.233"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            812..1024
                     /locus_tag="U4A80_002850"
     CDS             812..1024
                     /locus_tag="U4A80_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976431.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1014..1409
                     /locus_tag="U4A80_002851"
     CDS             1014..1409
                     /locus_tag="U4A80_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976432.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
BASE COUNT          499 a          211 c          239 g          478 t
ORIGIN      
        1 aataattact tgattatatt ggtttcccat ataattgaag gctgtcctaa tttttaccta
       61 ctatatgatc ataattgatt aactcttata tatttccgaa aattatcttg aagatttcta
      121 ttggtatgga gaatatccat ttcattgcca ataaggatat aaattctcaa aataacaatc
      181 gatgtctgta ttaaacaagc ctagaatggt aaatctgatt cgccaaaacc attggattgt
      241 atcgatttgc tttacgcgaa cgccacctac ccattttata aaaataaaat gggcaaaaga
      301 tctttctttt tgtcatacaa aaagaagaaa aaaaagagac gattttcttc ttccaaaatc
      361 gtccctcttt tttctaaaac catggatgaa cgaacaaaaa atctctcgta ttttgtttgt
      421 tcatccacta aaaatatgcg aacagtatgg tttcattcaa tcctgttctt acaagtcaaa
      481 agcatcgaat cgtccttgga cgattcaaca aaaaaagtag tcgtttttgg atgactacta
      541 agttctaaac attcattacc caaaatcaat ttggtctaac atcgtttctc gactggcttg
      601 atctaatcgg ctcttgacag ggctttttct tttctgttga tagcagataa gaggaaataa
      661 gaataagtgt gttcatgtgt cataaaaaca gtaactaaaa cattaaaatc aaaatttata
      721 tggaaagctt ccttgacatt ttgtgctcgc aggagtatta taaaagcacg aaaggaaagt
      781 aaactttcca ttttttagaa aggagcatgt tgtgaaaaat cgtttggaag aattacgaaa
      841 acaaagagga attaaacaag aagatttagc ttctgctctt gaagtatctc gtcaaacaat
      901 aggttcttta gaaaatggac gttataatcc ctctattata ttggcattta aaattgcaag
      961 gtactttcaa atgagcattg aagaaatttt tatctatgag gaggaaagtg aagatgaaaa
     1021 ataagggtca gatttggtat atcggttata ttgtttcgtt gtttttgatt gtgacaattt
     1081 tggttacaga ttttcctaaa atggtagata ttggacttgc tattttattc agtgccgttt
     1141 ttggcgtttc tcatgttcaa attttgcatg ataaaatgat taaaaatgat gcagactaca
     1201 aaatcaatgt tatggacgaa cggaatatca taatcaaaga aaaagcggga aatataacaa
     1261 atatgtttaa cacaatactg ttaggggtgg caacagttat atttatttgt ttagactaca
     1321 tcattccagc tattattgtc ggaagtatca ttgcaatcca gccgattatt ttaatcatta
     1381 tcagtagtgt gcttgagaaa aaaatgtaga aataaaggtt catacaa
//
LOCUS       DASJRQ010000225         1422 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJRQ010000225 DASJRQ010000000
VERSION     DASJRQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.234"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..>1422
                     /locus_tag="U4A80_002852"
     CDS             119..>1422
                     /locus_tag="U4A80_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1976433.1"
                     /translation="MIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTK
                     GHSENNLRQINTFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVL
                     ETPVYVTSKSEFKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDP
                     NYRYSFKEWQDWSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKG
                     LIGRNNVMFTLSLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQ
                     GANREYITILCKAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISE
                     KSDVYKPYLVTTKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVK
                     SLLLSIIKVKKEEKESYIKALTNSFDLEHTFIQETLNKLAERP"
BASE COUNT          511 a          231 c          268 g          412 t
ORIGIN      
        1 aactgtgctt catgacggct tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat
       61 cactaccaag ccaggtaaaa gcaaaggggc tatttttgcg tatcgctcaa aatcaagcat
      121 gattggcggt cgtggtgttg ttctgacttc cgaggaagcg attcaagaaa atcaagatac
      181 atttacacat tggacaccca acgtttatcg ttatggaacg tatgcagacg aaaaccgttc
      241 atacacgaaa ggacattctg aaaacaattt aagacaaatc aataccttct ttattgattt
      301 tgatattcac acggcaaaag aaactatttc agcaagcgat attttaacaa ccgctattga
      361 tttaggtttt atgcctacta tgattatcaa atctgataaa ggttatcaag catattttgt
      421 tttagaaacg ccagtctatg tgacttcaaa atcagaattt aaatctgtca aagcagccaa
      481 aataatttcg caaaatatcc gagaatattt tggaaagtct ttgccagttg atctaacgtg
      541 taatcatttt ggtattgctc gcataccaag aacggacaat gtagaatttt ttgatcctaa
      601 ttaccgttat tctttcaaag aatggcaaga ttggtctttc aaacaaacag ataataaggg
      661 ctttactcgt tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac aagtagatga
      721 accctggttt aatctcttat tgcacgaaac gaaattttca ggagaaaagg gtttaatagg
      781 gcgtaataac gtcatgttta ccctctcttt agcctacttt agttcaggct attcaatcga
      841 aacgtgcgaa tataatatgt ttgagtttaa taatcgatta gatcaaccct tagaagaaaa
      901 agaagtaatc aaaattgtta gaagtgccta ttcagaaaac tatcaagggg ctaataggga
      961 atacattacc attctttgca aagcttgggt atcaagtgat ttaaccagta aagatttatt
     1021 tgtccgtcaa gggtggttta aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt
     1081 gtcagaatgg aaagaagatt taatggctta tattagcgaa aaaagcgatg tatacaagcc
     1141 ttatttagtg acgaccaaaa aagagattag agaagtgcta ggcattcctg aacggacatt
     1201 agataaattg ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga ttaaaccagg
     1261 aagaaatggt ggcattcaac ttgctagtgt taaatcattg ttgctatcga tcattaaagt
     1321 aaaaaaagaa gaaaaagaaa gctatataaa ggcgctgaca aattcttttg acttagagca
     1381 tacattcatt caagagactt taaacaagct agcagaacgc cc
//
LOCUS       DASJRQ010000226         1374 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJRQ010000226 DASJRQ010000000
VERSION     DASJRQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.235"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..260)
                     /locus_tag="U4A80_002853"
     CDS             complement(<1..260)
                     /locus_tag="U4A80_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN1976434.1"
                     /translation="MVYFSIPQNTDSSWINVVNAAFDKFLEPKTFTISPFTFSKIAMK
                     GGAIIAVIVLLIDLYIISGKKNLRMGEEHGSAKWGTSKDIAP"
     gene            complement(335..>1374)
                     /locus_tag="U4A80_002854"
     CDS             complement(335..>1374)
                     /locus_tag="U4A80_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEN1976435.1"
                     /translation="QLFNMELIRAETKWYSFYQKNHRPPDYNFLQEEITSFGVNRLPM
                     FDRRNEGRVNDELSVDNFVHYHIQQLKLEKILSPIFFEKNDLRFMERYAKNKAKAYFN
                     ERDQYIKGYESFRISRNKIMQRAGYEQLKYEFVSDNMVQGYFKEEVVKLAVNDERVAE
                     FVLDEGKNLMRGLLQGLSKAGIAVVTVPAKAVHSQIKKQRTTGKQRYNQLKKHGSLEH
                     IDVSKTDLKPLANELKKYKVDFAVKRNKETGKIHVFFKAQDRDTLNLALENVLQKFAN
                     EKDQNREQQQDKSNKDIEKSSADGKDKDKSLAEKLDKLEDKAQEINQQREQQREQLKE
                     KSKNKTKGQER"
BASE COUNT          401 a          264 c          179 g          530 t
ORIGIN      
        1 taaggagcaa tgtctttaga agtaccccat ttggcactcc catgttcttc gcccattctt
       61 aaatttttct tgccacttat aatatataaa tcaatcagta aaacaataac cgcaattatc
      121 gccccacctt tcatagcaat ttttgaaaaa gtaaatggag aaatggtaaa tgtcttaggt
      181 tctaagaact tatcaaatgc tgcatttact acatttatcc atgaagaatc tgtattttga
      241 ggaatcgaga aataaaccat aaaaaataaa gtacctaaat aaaagaaaaa agcagcaagt
      301 acaataccaa aaacaaggtc ttttttggta atattcatcg ttcttgcccc ttagttttat
      361 ttttcgattt ctcttttagt tgttctcttt gttgttctct ttgctgattt atttcttgcg
      421 ctttatcctc taatttatct aatttttcag ctaacgattt atctttatcc ttaccgtcag
      481 cagaagattt ttcaatatct ttattagatt tatcctgctg ttgttctcta ttctgatctt
      541 tttcattagc aaatttttgt agcacatttt ctaatgctaa attgagagta tctctatctt
      601 gagctttaaa gaaaacatgt atttttccag tctctttatt tctttttacg gcaaaatcta
      661 ccttatattt ctttagttca tttgcaagtg gctttaaatc tgtctttgaa acatcaatat
      721 gttctaacga tccatgcttt ttcaattgat tatagcgctg ttttcctgta gttctctgct
      781 ttttaatttg tgaatgaact gctttagctg ggacagtaac aactgctatc cccgctttgc
      841 ttagcccctg taacaatcct cgcattaaat ttttgccttc atccaataca aactcagcca
      901 cacgttcatc atttactgcc aatttaacaa cctcctcttt aaaataacct tgaaccatat
      961 tatcagaaac aaattcatat ttaagttgtt catatcctgc tcgttgcatg attttatttc
     1021 gagatattct aaaactttca tagcctttta tatattgatc acgctcattg aaataagctt
     1081 tcgctttgtt ctttgcgtat ctctccatga atcgtaaatc atttttttca aaaaaaattg
     1141 gggacagtat tttttcaagc tttaattgtt gtatatgata gtgaacaaag ttgtctacac
     1201 ttaattcgtc attcacacgt ccttcatttc ttctatcaaa catcggcaaa cgattgacac
     1261 caaatgacgt aatctcttct tgtagaaagt tataatcagg tggacggtga tttttttggt
     1321 aaaaagaata ccacttagtt tccgctcgaa tcagttccat attaaaaagt tgtc
//
LOCUS       DASJRQ010000227         1367 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJRQ010000227 DASJRQ010000000
VERSION     DASJRQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.236"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..>1367
                     /locus_tag="U4A80_002855"
     CDS             74..>1367
                     /locus_tag="U4A80_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1976436.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          434 a          270 c          259 g          404 t
ORIGIN      
        1 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
       61 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
      121 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
      181 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
      241 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
      301 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
      361 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      421 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      481 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cattgaatcc
      541 tttcaaacag actttgccaa aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt
      601 tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat tggatattgt
      661 cgaaaatcga cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg
      721 tgaagccgtt caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa
      781 gcttttcccg aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag
      841 aactttcttg aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc
      901 tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc
      961 aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag
     1021 tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata
     1081 ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac
     1141 tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt
     1201 tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt
     1261 aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact tttacaattt
     1321 caaactacga attatgattc gtcatggcaa aacattttta accaaat
//
LOCUS       DASJRQ010000228         1366 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJRQ010000228 DASJRQ010000000
VERSION     DASJRQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.237"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(110..409)
                     /locus_tag="U4A80_002856"
     CDS             complement(110..409)
                     /locus_tag="U4A80_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1976437.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            760..>1366
                     /locus_tag="U4A80_002857"
     CDS             760..>1366
                     /locus_tag="U4A80_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1976438.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFAR"
BASE COUNT          453 a          222 c          196 g          495 t
ORIGIN      
        1 gtttctttat ttagaataaa aaaatacaga ctctgttcaa ataaatgaac ctagcctgca
       61 ttatgagatt gactatactt tttaattcat tgagatgaat aactcgtttt tataaatgcc
      121 caaaagtaat cggtgccgaa gccattcctc gacctaattc tttttcaaat tttcctattg
      181 ttgatatctc catataaaac ggtgatactg gtttagacaa ttctcccctg attgctaacg
      241 gacgtaaact acgttccaaa ttatttatga ctttaggata atactgacct ttttctaagc
      301 ctgtctttgc gtctatcaaa agtatacgtt ccttttcttt gatctctgga tttaaaacga
      361 gatcatataa ttttagtacc atgttttcta tttcttcact tttagacatc acgatttcct
      421 cccaaaaata tatttcttcc cactacattc tataaacaaa gtatacgcta catctagatt
      481 tttttaaatc attactggat tgttaaaacc tttcaataca ctgttatggc aacgattttt
      541 attttaaaaa cattctaaat taaatcagta aaaattttta ttctaagatt tttattagaa
      601 ttacgcctaa tttgtgtctc tcagggcaat tctaagaacg tttcaagaga tagcgacact
      661 atcgttatcg aaaaaggcga aaacctatct cattttctgc taatgtaagt tggtttagct
      721 tttgttaata tgactttatg aaaatggagg taattaaata tgatttttcc taaagttaat
      781 gaagcaagct tgaataaagg atcctttttt catatctatg aaaacgaaga ttttgaactg
      841 tataatatcc tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca tccaattatt
      901 tacaaatcta ttttcaagta tgcatttttt gcttccaatg attttcaaag aatggagatt
      961 accgactgta tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa aagcatgttg
     1021 catcgcgtga aatttgtcaa ttgtaaatta acaggtacta aattgatgga aagttatatt
     1081 gggaacacgt tattcgagaa ttgtaaaatg gactatgtaa atttatctgg ttcaaacata
     1141 aaagaaagta cctttgagca ctctgtctta tcaagcgcag attttgtaga ctgttccctc
     1201 actaaaataa tgttttctac taatgattta gaaaatgcta gcttttttga tactgattta
     1261 aaaaatatcg atttaagtag caactccttt gaaatgattg aagtacgctc ggaacatcta
     1321 aaaaattgta cgcttaatca gttgcaagca cttggttttg ctagaa
//
LOCUS       DASJRQ010000229         1362 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJRQ010000229 DASJRQ010000000
VERSION     DASJRQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.238"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1362
                     /locus_tag="U4A80_002858"
     CDS             82..>1362
                     /locus_tag="U4A80_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1976439.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DASJRQ010000230         1333 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJRQ010000230 DASJRQ010000000
VERSION     DASJRQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.239"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..765
                     /locus_tag="U4A80_002859"
     CDS             82..765
                     /locus_tag="U4A80_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976440.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(802..1299)
                     /locus_tag="U4A80_002860"
     CDS             complement(802..1299)
                     /locus_tag="U4A80_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1976441.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          420 a          235 c          223 g          455 t
ORIGIN      
        1 taaatcaaca aaaaaacgca tatagtagtc tcaaaactcc caagaaatga ggactattta
       61 acaaactacg atggttacgt cgtgacaact gaacattttg acttagccaa tcctgttact
      121 aaagtcgatg atatccccga ttatgaaatg tacagtcaaa caatcgatag cttaaataag
      181 cgttttggca atcgtgtttt aaaaggcatc aaaatcggtt acatcgcttc tgaaaaggat
      241 cggatcatcg actatttagc cgataaagat tacgatttaa aattactcag tgtgcatcat
      301 aatgggcaat ttgattattt agatgacgaa gtcaaagata tggatcctgc tattgtgatt
      361 cctcagtatt ttgcacaact ttcagaagca ctagttgtta tagaagcaga tgtctttgct
      421 cattttgact atggttttcg ggtatttggt ctatccgttg ctgaatttaa acaatatgaa
      481 gcgcagtttt taccgattct agatcaagta atcaaaaata aattggcatt cgaattaaat
      541 gctaagagcg cttacttgta tgacaattta gcgttatatg aatatgtcat cgatttatat
      601 ctttcacgtg gtggtacctt gtttagtgtt ggatcagatg ggcattacct tgaacatttc
      661 cgtttccatt tcgatgatct ttttgctctg ttaaaagcaa atggtgtgac ggaattagcg
      721 atttatcaaa aaggaaagcg aattatggtt ccattaccag tctaaaaaaa cgtcctcagc
      781 atcaaaagtg aggacgtttt tttagatttc tttttctaaa atcggacaaa tacgatcttg
      841 ttttcctaaa ggaaaatgtt tgatcccgat ttttttaaat ccatgacgtt cataaaaacg
      901 aatacctgca tgattttttt cataaacagt caaacgtaaa taacgttttt gtttcgtttt
      961 agcataacga ttcgcttcat cgagtaaccg ttgccctaac cctttgtttt gataatcttt
     1021 gagcagatag agccgttgaa tctctatagc atctttttca tcattgactt tcatataacc
     1081 aatgagtatt tgatgatcct taataccaat aaatttactg gttgactgtg ccaattctac
     1141 tgccaatatt tcagttgtca attgtgtctg taaatatcct tgcatatcaa gtggatctgc
     1201 gccatcgata aacgattcat aaaaactgac aacacttaca acttgtaaag catctagatc
     1261 atcataatct aaatacgcta gtgtgatttg attgttcatc taactcctcc ttttgacata
     1321 gtatactata aat
//
LOCUS       DASJRQ010000231         1293 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJRQ010000231 DASJRQ010000000
VERSION     DASJRQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.240"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..261
                     /locus_tag="U4A80_002861"
     CDS             <1..261
                     /locus_tag="U4A80_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HEN1976442.1"
                     /translation="PTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELLQQLA
                     ELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            271..759
                     /locus_tag="U4A80_002862"
     CDS             271..759
                     /locus_tag="U4A80_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1976443.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1055..>1293
                     /locus_tag="U4A80_002863"
     CDS             1055..>1293
                     /locus_tag="U4A80_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976444.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYV"
BASE COUNT          545 a          226 c          191 g          331 t
ORIGIN      
        1 ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt tcaatctgaa
       61 aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt agaactttta
      121 caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat taacaaacaa
      181 atagcagata acaattccga tattacccca gaatttaaag aaaacttact agataatctc
      241 caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa agaattagca
      301 gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt acctagtaat
      361 tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac aacaattaca
      421 aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga ttcattacct
      481 accgattacc tactcaagga gattgaaact aagaataatc atattgaaga aaaagatagg
      541 caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga ccagcaacaa
      601 caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact ccagctaacc
      661 tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac tcaaaaagga
      721 caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt ttattcataa
      781 aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga tctcacgtcg
      841 ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg tgctaggtag
      901 gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc attgtggata
      961 gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg tatgatatac
     1021 ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc ggaatattga
     1081 tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa aaactggcaa
     1141 aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg accaggcact
     1201 taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg atatacaagc
     1261 aaaaaatact gaaacattga acgactatgt tca
//
LOCUS       DASJRQ010000232         1261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJRQ010000232 DASJRQ010000000
VERSION     DASJRQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.241"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..464
                     /gene="spx"
                     /locus_tag="U4A80_002864"
     CDS             54..464
                     /gene="spx"
                     /locus_tag="U4A80_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1976445.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            478..834
                     /locus_tag="U4A80_002865"
     CDS             478..834
                     /locus_tag="U4A80_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1976446.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          458 a          178 c          236 g          389 t
ORIGIN      
        1 attttaaaag aaattactgt agaattggat tataattaaa aaggagtgag aaaatgataa
       61 aagtatatac ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga
      121 atcaacttga atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca
      181 aaaagatctt ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa
      241 tttacgaaaa gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat
      301 tcaaagaata tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa
      361 tcggatttaa tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca
      421 gaaatctgat ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg
      481 gaagtgaaag tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta
      541 gaacaccatc aattgcctta tcaatttgtt gacttagaag aacgcgattt aagtacagaa
      601 gaagcagaag aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg
      661 tctgaggctt ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt
      721 tttttatgca cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt
      781 ctatttattg gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta
      841 actgttttaa tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc
      901 aagcgtgtta tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt
      961 atgtggtcaa aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta
     1021 aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt
     1081 aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagttg
     1141 ttaaaaaaga accacttgtg aagcttgatt ctatcgaaaa aaacttcaaa aaagttcttg
     1201 acaaataaca aatgataagt tatgataaat gagttgctgt aagatttcga aaaacgaaat
     1261 c
//
LOCUS       DASJRQ010000233         1253 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJRQ010000233 DASJRQ010000000
VERSION     DASJRQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.242"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..390
                     /locus_tag="U4A80_002866"
     CDS             34..390
                     /locus_tag="U4A80_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1976447.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            380..646
                     /locus_tag="U4A80_002867"
     CDS             380..646
                     /locus_tag="U4A80_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1976448.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            805..1083
                     /locus_tag="U4A80_002868"
     CDS             805..1083
                     /locus_tag="U4A80_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976449.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(1080..>1253)
                     /locus_tag="U4A80_002869"
     CDS             complement(1080..>1253)
                     /locus_tag="U4A80_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1976450.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT          485 a          169 c          247 g          352 t
ORIGIN      
        1 ataatataaa atcaatatat gatataattg ggtatggaaa aaatatcttt tgaaacattt
       61 aaaagaccca atggtcatga tgaattttta gaatggttgg aaacattacc aaaaaaagat
      121 agtgcgaagc ttttacgtac aatagaagaa acagaaaaga atgggatgct agtagctcaa
      181 cggttgaaat gggtgaaaaa attagacact aatttgtacg aattaagaag taaggtagga
      241 tcaaacatcc aaagagccat ctatttccat gtggacaatg gaaggtatgt cattactcac
      301 ggatttacta aaaaaacgga gaaaacacca ataactgaac tcaagcatgc aaaagaactt
      361 agagaggagt ggttcacaaa tgatgattaa aaaattgatt gaagaaaaga aaaaagataa
      421 agattttgca gaagcatatg accgtgaagg aagaaaactc gaaactgcgg ttgctcttta
      481 tcatgctcgg gaggaagcag ggctaactca agcggagctt gcacaaaaag cacatacaac
      541 tcaatctact attgctcgaa ttgagagagg ggataacgta tctttcgata aacttgatga
      601 tatcgccagg gcattaggta aaaagctggt agtagaattt gcttaaaagc gagttgaaaa
      661 tatatctata ataggctcct aactaatcaa ttagtttagt gggccttttt ttactagata
      721 gataaaggtt gtactatagt gtaaaagaca ataaatttta tactaaaatt actttaaaga
      781 gaactagaga gaaaggtaca ataaatgaaa accataaatc ttataaaagt atttatagca
      841 tcaccaggtg atatagcagc aaaaaggaat gaagtagaag aaaccatata tagatggaat
      901 atagaaaata cagataatac aaaagtgatt ttgatgccta tacgatggga aactaatgcg
      961 attcctactt atgtaaataa tagctctggg caaagtgtta ttaataaaca aattgtagaa
     1021 aatagggttc tgctgcaaag ttttaaaaat aaaatcagtt tttgtattat ttcttcattt
     1081 taagctggaa ttcccaataa tatcctgatt tcagtgcaga ctgaaaagcc gaagagagtg
     1141 ccttcttttc ggcttttctt gtagattcct cgaatggttt ccatcccttt aattgtggtg
     1201 gaagctgtac gtagactttg atacaattta tgtcgacgtt ttatcgatcg atg
//
LOCUS       DASJRQ010000234         1206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJRQ010000234 DASJRQ010000000
VERSION     DASJRQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.243"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..1148)
                     /locus_tag="U4A80_002870"
     CDS             complement(join(22..920,920..1148))
                     /locus_tag="U4A80_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1976451.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          339 a          211 c          194 g          462 t
ORIGIN      
        1 gacacatttt agtagagacg gttaagccat tttttctttt gctaattctt caaattcctg
       61 tggggttaga tagtgaatcg agctatggat tcgatttcgg ttataaaatc cttcaatgta
      121 gctaaatagc gctcgatttg cttcttcaaa atctgagtag ctagtcgtgt agacttcttc
      181 ttttttcaat gaggcatgga aagattcgat tccagcatta tcataaggtg ttccttttct
      241 actataagaa tgccttattt tgttggtttt aagccattgt tccacttctc ttgctgtgta
      301 ttggctgccc aagtcagtgt gtagaatcat tccttctgga atgtgataat gtatttttgc
      361 tttattcaat gtttgaatga cacaatccac agtcattcgt tttgaaaatg tataactaat
      421 aattcttttc gtgtgtaaat ccatgattga agataagtaa caccaaccat ttttcttagt
      481 aggaatgtac gtaatgtcag ctgcccattt ctcacagatc gtttcagtag aaaagtcctg
      541 atttaagaga ttctcttttg aaatgatcgg tttattagac ctttgaggtc tatatttttt
      601 aactacaatc gaccttaaat ttaattgttt cattagcttt tggacatgtt taagagatac
      661 tgaaatccct tcttttaata aaacttgatg gatctttgtg gcaccatagc gttgtttatt
      721 cgtaaaaaaa attgttgaaa tcttcttttt caacttatta tttctgattt ctgtagctgt
      781 caacggttta ttcttataaa aataatagac acttctaggg atttctaata accgacacaa
      841 caaagaaaca gtgtaatcct tacaccattt ttcaataaat tgaacaagcg aatttaggtt
      901 ttccgagaga atatggctgc ggcttttttt aagatttcaa gttcctcatt taaacgagca
      961 ttttcttttc tcagttcagc tacttctttt ccagtcagtc ctgtggactg atcaggtaaa
     1021 tataaattct tccatttgta aatcgttgct tcagataagc catattcttt cgctaaccct
     1081 cgaacagaac gtccagtttg attcagttca acaatcattt gtttaaaatt ttcttcatat
     1141 cgggtcataa aaattcatcc tctcgtagat aagtttaatt tattcttgtc tctacgaaaa
     1201 gctgtc
//
LOCUS       DASJRQ010000235         1201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJRQ010000235 DASJRQ010000000
VERSION     DASJRQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.244"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..972
                     /locus_tag="U4A80_002871"
     CDS             19..972
                     /locus_tag="U4A80_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1976452.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            944..>1201
                     /locus_tag="U4A80_002872"
     CDS             944..>1201
                     /locus_tag="U4A80_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN1976453.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYN"
BASE COUNT          430 a          187 c          231 g          353 t
ORIGIN      
        1 aggaaattgg agggaactat ggaaaactta cggactgcat ttaaaacatc agataaacta
       61 ggaaaagttg taatgcttac aggtagaaaa ggcggcattg gtaagactac agacaatgat
      121 ttattagcga ttgtttctag tcaacttttt gaaaaagacg tgttgctaat tgattatgac
      181 caacaaagaa atactacttc caacattgga tcaacgtatc aaattactag ctttgatcga
      241 tccatgtctg cagcaatcaa aaaaggggac tgggtttcag gtattactca agtaagtcca
      301 catttatata ttatggctgg ctctcctgga tctgaagaac ttaatgaata cttgtccgaa
      361 aaatatcctg atcgtcgtaa gagatcattg gccttcatta aacctttaga agaattgaga
      421 aaaaatttcg attatatctt tatcgactgc ccaccatcaa cagataatgt ggttcgtgca
      481 ttcttaactg ctgcagatta tattattgca atgcaagaat taaaacggta cgcaatggaa
      541 ggaacagaag attttattaa taaagtttta gttccgattg tcacgaattt tgaagagtct
      601 catcttcaaa tcattggaat tttgcctgtt cttttctcag tgcgacgttc ctctcaacac
      661 gcaaactatc aaaaaacaat tgataagtat ggtgaatcaa acatttttaa aagcattgtt
      721 aaaggctcgg atcggttaga aatgtatggt gagaatggta tccaattaaa tgattatgta
      781 gatcgaagat ggtgggctat ttttgcagac atctttacag aattagaaga aagaattgaa
      841 tactatgaac aacatggtga tattgaagga tttgattata cacctaaata tgctgactca
      901 atggccaatc atatcctacc tcttggaaag gagattaaac taaatggcat tattaccaac
      961 gaacaaagat aagaagaaaa ttggtgctga agatatttca attaaagatg atattaatgt
     1021 cgagttaggg gaacgaaagt acactgcgaa agaaagaaaa ccagttcaag tagatcctcc
     1081 cgtactaaaa accattcggg atcttagtta tgcaaaagat attcctatgt atgaaatagt
     1141 caaacttgca atggacgcct atgttgatac tttaaacgac gaggaacgga ttatatataa
     1201 t
//
LOCUS       DASJRQ010000236         1171 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJRQ010000236 DASJRQ010000000
VERSION     DASJRQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.245"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..306
                     /locus_tag="U4A80_002873"
     CDS             1..306
                     /locus_tag="U4A80_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976454.1"
                     /translation="MINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHK
                     FKENDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            306..>1171
                     /locus_tag="U4A80_002874"
     CDS             306..>1171
                     /locus_tag="U4A80_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEN1976455.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIFQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGIIADV
                     QTKEKKTNSPSL"
BASE COUNT          435 a          194 c          237 g          305 t
ORIGIN      
        1 ttgattaata gccttgtcat tgaattgtac aagtatcaag cagaatcaga acgaaaaaaa
       61 attaaggaac gtcaggcaca aggaatcgaa attgctaaga aaaaaggcaa attcaaaggt
      121 cgtcagcata aatttaaaga aaatgatcca cgtttaaagc atgctttcga tttgtttttg
      181 aatggtttat ccgataaaga agttgaagaa caaactggaa tcaatcgccg aacgtttaga
      241 aggtatcgag caagatataa cgtgacagtc gatcaaagaa aaaacaatga aaagagggat
      301 agttaatgag tacggttatt ttagctgaaa aaccaagcca ggcattagcc tacgcaagtg
      361 ctttaaaaca aagcaccaaa aaagacggtt attttgagat caaagaccca ctatttacag
      421 atgaaacgtt tatcaccttt ggttttgggc atttagtgga attagcagaa ccaggtcatt
      481 atgacgaaaa gtggcaaaat tggaaacttg aatctttgcc gatttttcct gatcgatacg
      541 attttgaagt tgcaaaagat aagggaaagc agtttaaaat tgttgcagaa cttctcaaaa
      601 aggcaaatac aattattgtt gcaacagata gcgacagaga aggtgaaaat atcgcctggt
      661 cgattatcca taaagcaaat gccttttcaa aagataaaac atttaaaaga ctatggatca
      721 atagcttaga aaaagatgta atccgaagcg gttttcaaaa tttgcaacct ggaatgaatt
      781 actatccctt ttatcaagaa gcgcaaacac gccaaattgc cgattggttg atcggcatga
      841 acgcaagccc tttgtatacg ttaaatttac aacagaaggg cgtacaaggt acattttcac
      901 taggacgtgt tcaaacgccc accttatacc ttatttttca gcgccaggaa gccatagaga
      961 attttaaaaa agaacctttt ttcgaggtgg aagctagtat aaaagtaaac caagggtcgt
     1021 ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca agaggagctt ttagcttttg
     1081 tttcttctaa acaagctaaa ataggcaatc aagaggggat aattgctgat gttcaaacca
     1141 aagagaagaa aacgaatagt ccgagtttgt t
//
LOCUS       DASJRQ010000237         1143 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJRQ010000237 DASJRQ010000000
VERSION     DASJRQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.246"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..351
                     /locus_tag="U4A80_002875"
                     /pseudo
     CDS             133..351
                     /locus_tag="U4A80_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            611..865
                     /locus_tag="U4A80_002876"
     CDS             611..865
                     /locus_tag="U4A80_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1976456.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            866..>1143
                     /locus_tag="U4A80_002877"
     CDS             866..>1143
                     /locus_tag="U4A80_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1976457.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVG"
BASE COUNT          403 a          171 c          225 g          344 t
ORIGIN      
        1 tagatacaat atcgatcagt tttgaggaaa tgaaattcta gatatttttt gctacataga
       61 gttactttca atatttttta acgtaattag gcagaagtca ctcatcctct ataggtttgt
      121 gatgaaattg ctattgatcc atttaagtaa gagcaaaaaa ctggaggttt atcaggtaat
      181 tggtctaatg gatttaagta tgcggtcacc gtgtatagag ttgcttatga gattcaagaa
      241 aataaagtta ttccagtatt gctatatgga acacataaca atttttatga attagccgag
      301 aacactcgca actttattcg tgtagatgaa tcggcttcgg ttgagttata actttagcta
      361 tatcaatcaa ccgaccgaca tatgttaaat ctatatataa taaaaatacc tacttaaatg
      421 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
      481 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      541 aacaccgttg gcaattgtcg ttataacatg ttataattta tttatcaaaa actaaaagaa
      601 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      661 ccttccaagc tctcttggat taaaaagcgg agacactatc caagccaaac aagaaggcaa
      721 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      781 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      841 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      901 ccagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      961 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
     1021 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
     1081 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
     1141 agg
//
LOCUS       DASJRQ010000238         1139 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJRQ010000238 DASJRQ010000000
VERSION     DASJRQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.247"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..561
                     /locus_tag="U4A80_002878"
                     /pseudo
     CDS             52..561
                     /locus_tag="U4A80_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            562..879
                     /locus_tag="U4A80_002879"
     CDS             562..879
                     /locus_tag="U4A80_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1976458.1"
                     /translation="MLSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYIIQKDK
                     VSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTAKGMGR
                     LIS"
BASE COUNT          433 a          178 c          228 g          300 t
ORIGIN      
        1 tttttgagcc aaaagtaagg aattacaaaa agagaaagaa gagaaggaag aatgaaaaag
       61 gtattatttg cggttagttt agttgttaat atagcactag caggaggatt attttatacc
      121 attacagaaa tgaataagct tgatgaccaa cgggaaagct tagcaatcag taacgaacaa
      181 tacaaaagga agctagatta agctagcagc accaacgtgg acactcaaga agccgtagaa
      241 tcctctacaa ttgtttatag caatgaatcc agtgaaaccc taacaaattc ggaaagaagc
      301 acagctccag ctacagaaac tgtttctcaa aaagctacta taacaatagg aagtaaagta
      361 ttcacgttta tagtaaatgg cttgacaata gaacacgatt ctagcagaac aggaaaaatg
      421 accgagcttt ctcacgagtt tccagtgatt gttactactc ctagtgctac ccttaacggt
      481 atattggtgc aagacaacaa tgataataat tgctatataa tggtagacgg tcaaactcta
      541 tacagaggaa cataccaata aattttgagt ctcaaagata tttgtcacta cggtaaacgg
      601 tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg
      661 cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt
      721 gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct atatccaaca
      781 atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa tggagtcttg
      841 cgttgtaccg caaaagggat ggggcgttta atcagctgaa ttgatcattc tcataacttg
      901 tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaaag aacttagaat
      961 cattagaaaa ataaggagta gacttgatct atcaacaatt tccttcgggg agtgacgggg
     1021 tagattttta cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg cttgtttgga
     1081 tagtggtgaa atgaatttgt gtaaataata tacacagagt aaacaagagg gaatttaga
//
LOCUS       DASJRQ010000239         1117 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJRQ010000239 DASJRQ010000000
VERSION     DASJRQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.249"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..226)
                     /locus_tag="U4A80_002880"
     CDS             complement(<1..226)
                     /locus_tag="U4A80_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976459.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVL"
     gene            complement(232..462)
                     /locus_tag="U4A80_002881"
     CDS             complement(232..462)
                     /locus_tag="U4A80_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976460.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(712..912)
                     /locus_tag="U4A80_002882"
     CDS             complement(712..912)
                     /locus_tag="U4A80_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976461.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          399 a          182 c          161 g          375 t
ORIGIN      
        1 ctagcacatc agtcacgata tcaaacaaaa aatatacgtc tttcttcatt ctagttttcg
       61 cagcagggat tttaaagtag ttcttattag aatagtagag attaattaat aagctatctt
      121 cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat
      181 tcgaaggatc aaatttaaac cataattgcg tctcattaaa aagcataacc attactccga
      241 tctattttta aacttagtct ctacctctaa tatatatcga gtttttattt tgccttcaga
      301 gaatactgtt tctttttttg cagttacagg ttgtttattt tcggaaaaag ctaatatagc
      361 taaaattgaa acatccatct ggaatttgtc tttttcgttg ctttgctcat aaaaatctgc
      421 atattcatca ctaatatttt ttctaataaa ttcttccatc ataaagatca cctcgaaaag
      481 agtatacgaa caaacgttct ttttgtaaag gtggaattta ttatactaaa taaataggtg
      541 aacaagtact tgtgccaaat tatgtgccaa aaaaaatcga atttaataaa acacaaacaa
      601 aaagaaatcc tattatgata acatttctta taataacaaa cacaacagaa gacgtgtaat
      661 agttagtcag gaacgtacaa ataacccctg tatcctttgc ggtacagggg gttattttgt
      721 ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat
      781 atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat caataaacag
      841 tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt cgacggtgta
      901 gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt ataccagtcc
      961 tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc tttacgtaaa
     1021 gccaatggga atgaactgga gatgactttt tattattttt agtaatagct gatgggttaa
     1081 tacttacaac tttccttttc aaagtaccat ataaaaa
//
LOCUS       DASJRQ010000240         1106 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJRQ010000240 DASJRQ010000000
VERSION     DASJRQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.250"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4A80_002883"
     CDS             <1..1087
                     /locus_tag="U4A80_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1976462.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DASJRQ010000241         1089 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJRQ010000241 DASJRQ010000000
VERSION     DASJRQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.251"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1008)
                     /locus_tag="U4A80_002884"
     CDS             complement(<1..1008)
                     /locus_tag="U4A80_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1976463.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RI"
BASE COUNT          336 a          218 c          167 g          368 t
ORIGIN      
        1 gatacgattt tttttacttt tttcaagaaa ataataattc agaatattct gactatattt
       61 atgcaacact agtgacttta ggagaaacat caatagcctt gctaaagttt ttgctttcgc
      121 taaagagagt attttcgata atttgttttt ttctaaaata cctcgttcct caatatttgg
      181 gatatggata tgtataggga ttcttcgcat aaaggtttcc agaaaatcct ctttgccttc
      241 tgtagtcgca aaaactaaac gtacattgga ggcgtgatgg atagtttcac cgactctaga
      301 ataagttccg ccatccatat aaacaaatag tttctcttgc ccttcaggag gcaatctgtg
      361 tacttcgtct aaaaacagta ctccaccgtc tgcatcttgc aacaatccat ttcgatcttc
      421 atctgcgccg gtaaaagcac ctttaacata gccaaatagt atccccgaca ataattcagg
      481 attattatgg tattgcgcac aattaagtac tttaaaagga gcattctcat gaataacgtt
      541 cacttttttc ataaactgat aagcagtatg aacaaaaaaa ctttttccaa ctccagtatc
      601 tccagtgatc atcataggaa gccctcttcc aggatagaag acagcagttt tcatttgttc
      661 caccgcatcg gataagctca tatcataccc aatcatcttt tgtagaatgg aatctgtaga
      721 attgggctcc aagtctgttt ttaattcaga tatactttcg tatacatctt ttaccggaat
      781 caaaaagtgc tcttcaaagc gctctttgat ggcaaataac actggacgag tattgatttt
      841 aaatagaacg ttctttgcaa ctaagtcatt ggcaagttta ctggcatttg ttcttcccgt
      901 tcttaaaaat tgcgacaggt catttgtgtc aaaaaagcac tcttctaaat tactgatgcc
      961 aatttcttca cattttgtgt ttgcataagt cactatatca tttttcatct tataaacagt
     1021 cctttctaaa cttctatccc ttaaataaat agcaaaaaaa aggaagcaat atcgatatgc
     1081 tgtcctctt
//
LOCUS       DASJRQ010000242         1073 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJRQ010000242 DASJRQ010000000
VERSION     DASJRQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.252"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="U4A80_002885"
     rRNA            <1..>1073
                     /locus_tag="U4A80_002885"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DASJRQ010000243         1064 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJRQ010000243 DASJRQ010000000
VERSION     DASJRQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.253"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..995)
                     /locus_tag="U4A80_002886"
     CDS             complement(39..995)
                     /locus_tag="U4A80_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976464.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DASJRQ010000244         1045 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJRQ010000244 DASJRQ010000000
VERSION     DASJRQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.254"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1018
                     /locus_tag="U4A80_002887"
     CDS             <1..1018
                     /locus_tag="U4A80_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1976465.1"
                     /translation="KEVYREKYYQMPKVFFTNEKYLNLSNDAKITYMLLKARFDYSVQ
                     NNWVDSEDNIYFIYTIEELMRLLNCREGKISKIKKELEAAGLLKQKKGRIKKANGKIE
                     TTPNLLYLGKPEVTSEDVFKINEEERLIPNPVNAKIANTEKDDDIKVIPVNAKIANTE
                     KASDTNSSPVNAKIADNILYSNNSLDTNRHNIDTDKDQLQDQLLLENFVDIMTDSSID
                     TFIPTNVLKLIKTFSNSYSEAQQTVKTIHNAKHKAQESKNTIIVYEEIEYYGVDADKG
                     LYMTLLKAYQKQKTEKVDNLQNLIFIYVKNWFAEKAIPAKLAHEKNAETNSLPAVNLE
                     NWLD"
BASE COUNT          443 a          157 c          163 g          282 t
ORIGIN      
        1 aaaagaagtt tatcgtgaaa aatactatca aatgcctaaa gtatttttta caaacgagaa
       61 atatttaaat ttatcaaatg atgcgaaaat aacctatatg cttttgaaag cacgttttga
      121 ttactcagta caaaacaatt gggtagattc tgaggataat atttatttta tttacaccat
      181 tgaagagtta atgagattac taaactgtcg agaaggtaaa atttctaaaa taaaaaaaga
      241 attagaggca gcaggactgc tgaagcaaaa gaaaggacgt atcaaaaaag caaacggaaa
      301 aattgaaact acgccaaacc ttttgtatct aggtaaacct gaagtaactt cagaggacgt
      361 atttaaaata aacgaagaag aacgactaat cccaaatcct gtaaatgcga aaatcgcaaa
      421 tacagaaaaa gacgatgata tcaaggttat tcctgtaaat gcgaaaatcg caaatacaga
      481 aaaagccagt gataccaata gttctcctgt aaatgcgaaa atcgcagaca atatattata
      541 ttctaataat tctttagaca ctaatagaca caatatagac actgacaaag atcagctaca
      601 agatcaatta ttgctagaaa attttgttga tataatgact gattccagca ttgacacctt
      661 tatcccaact aatgttttga aattgattaa aactttttca aatagttaca gtgaagcaca
      721 acaaacagtc aaaactatac acaatgcaaa gcataaagcc caagaatcaa aaaatacaat
      781 tattgtttac gaagaaattg agtattatgg agttgacgca gataaaggat tgtacatgac
      841 tttactgaag gcatatcaaa aacagaaaac agagaaagtt gataacctac aaaacctaat
      901 ttttatttac gtaaaaaact ggtttgctga gaaagctatt cctgctaaat tggcacatga
      961 aaaaaacgca gaaactaact ctttacctgc tgtaaatctt gaaaattggt tagattagaa
     1021 aaatatcgaa catatgtttg caata
//
LOCUS       DASJRQ010000245         1015 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJRQ010000245 DASJRQ010000000
VERSION     DASJRQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.255"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..831)
                     /locus_tag="U4A80_002888"
                     /pseudo
     CDS             complement(1..831)
                     /locus_tag="U4A80_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319352.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          302 a          230 c          118 g          365 t
ORIGIN      
        1 cataattaat agcttcagtt aagattgcca aactggtacc tccactacta ccaaatacac
       61 gtataggtac catcgtaaca tcccaattta ttccagcaac acctaattcg ttgtttgttt
      121 gagcaccaat aatcccggca atatgtgtcc atgtcccata gtatccatag gatctgtatc
      181 atcgttaaaa aaatcgtatc tcaaactttc atctacattt ccagaaagat cttcatgttg
      241 ataatcaatt cctgaatcaa tgaccgctac cttcacatcc ttagaaccct tggtttcttc
      301 ccaagcggat ggcatgctaa tctctcttaa attagcttgt ttactccaat taggatcgtt
      361 aagaataatt gatcgatttc ctaaagaatc agttgtattt ggctcctcaa catcattcaa
      421 cggctctagt tcagccacat agtttggctc tgcataattg accatatcag aagcattaaa
      481 tgtttttact gcctcttcca cactaatttc ttcgttcaat ttcactaata atacttgatc
      541 tcgcttatta taatcgcttt catttgtcct aagagtcaaa tctttttcca ccataacttt
      601 tacacccaat aaattttgcg gtttattttt ctctgttgaa attctttgtg tagcctttaa
      661 actgacaatt agctcattgg gtacatactc tatttgagag tcatctaatt ggctcataat
      721 cctttctttt tggttagtat ctgcagttac ttgtttagtg tgagccacga acccaaacat
      781 actcacccca acaaccaatc ctaaatatat tttcttttta aactttttca tctacatctc
      841 ctcctaatag ttttatttta gagttagctc tattacttat ttactaactc aacaacagat
      901 tatcatacac ttgttaatga aattacaata tctcattaaa acacacccta aaatcataac
      961 cgttcctatg ctcatttttt ggatttttta atcacgtaac cagaaaaata aatcc
//
LOCUS       DASJRQ010000246         1009 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJRQ010000246 DASJRQ010000000
VERSION     DASJRQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.256"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            577..>1009
                     /locus_tag="U4A80_002889"
     CDS             577..>1009
                     /locus_tag="U4A80_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976466.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYMFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT          357 a          134 c          172 g          346 t
ORIGIN      
        1 atgaaactag gcttttaaca ataattaggc aaaagtcgct catcctctac aggtgggtga
       61 ttaatgccgc tcgatatggt tgttaaccgt cggttactcg aagattgata tataagactt
      121 agtacctttt tattttgctc catgttatgg ccgaaggtct aaagatacaa tcattagtct
      181 agagaaaaat taagaaccgt aggaactacg gggatcgctt ggtgaataag agacactgct
      241 attagtgaaa aaataagata tcaagtacgc tctattccca aaaatttcgt gactttactc
      301 atgagaggtt caagtagtat atttttaatc aaaattgacg taacttttaa taaatactac
      361 ctaaataagg tagtatttat ttttgtgttt taaaagagga agtccaatat atctatggga
      421 aacttcttaa atattatgtt ggtacatgaa aatatacatc cctgctagac tatttacaac
      481 attttacttt gtacgtattc ttgttttaaa aggaatggga taaaatatag gtacaataac
      541 tatttgatgc taaatataat taatggagga gagatagtga acatacttaa tgaaaaaaga
      601 tatgtatggt ttgctggtaa aaaatatatt actactttca aaagattatc cgagttaatg
      661 cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt ttaattttgt tcaatcttca
      721 gtatctactg aaagtatcta tttttcaatt ctttctgaga gtaggctttt tcattttaga
      781 ataagttctc atgtaaagaa aactgatgtt gatatatata tgttccgtac aaataattat
      841 aaaaatttaa atgatttgag tagaagtatc agtgaatatc tcaaggggaa tagctatctc
      901 aagattcgat tttcacatat attaatttta ctaccatatt tgaatgagaa tttcacccta
      961 attcaagtag ataagtcttt cattgcctca ggtaatcaag gaaatatga
//
LOCUS       DASJRQ010000247         1006 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJRQ010000247 DASJRQ010000000
VERSION     DASJRQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.257"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..97)
                     /locus_tag="U4A80_002890"
     tRNA            complement(25..97)
                     /locus_tag="U4A80_002890"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:His,seq:gtg)
     gene            complement(100..173)
                     /locus_tag="U4A80_002891"
     tRNA            complement(100..173)
                     /locus_tag="U4A80_002891"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Trp,seq:cca)
     gene            complement(180..260)
                     /locus_tag="U4A80_002892"
     tRNA            complement(180..260)
                     /locus_tag="U4A80_002892"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Tyr,seq:gta)
     gene            complement(262..337)
                     /locus_tag="U4A80_002893"
     tRNA            complement(262..337)
                     /locus_tag="U4A80_002893"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(302..304),aa:Phe,seq:gaa)
     gene            complement(373..445)
                     /locus_tag="U4A80_002894"
     tRNA            complement(373..445)
                     /locus_tag="U4A80_002894"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Asp,seq:gtc)
     gene            complement(455..527)
                     /locus_tag="U4A80_002895"
     tRNA            complement(455..527)
                     /locus_tag="U4A80_002895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Val,seq:tac)
     gene            complement(543..614)
                     /locus_tag="U4A80_002896"
     tRNA            complement(543..614)
                     /locus_tag="U4A80_002896"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(579..581),aa:Glu,seq:ttc)
     gene            complement(629..718)
                     /locus_tag="U4A80_002897"
     tRNA            complement(629..718)
                     /locus_tag="U4A80_002897"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(682..684),aa:Ser,seq:gga)
     gene            complement(758..831)
                     /locus_tag="U4A80_002898"
     tRNA            complement(758..831)
                     /locus_tag="U4A80_002898"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(795..797),aa:Asn,seq:gtt)
     gene            complement(841..956)
                     /gene="rrf"
                     /locus_tag="U4A80_002899"
     rRNA            complement(841..956)
                     /gene="rrf"
                     /locus_tag="U4A80_002899"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          274 a          273 c          226 g          233 t
ORIGIN      
        1 tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag
       61 ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg
      121 aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat
      181 ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt
      241 agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag
      301 cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa
      361 taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      421 atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg
      481 accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa
      541 aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc
      601 ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag
      661 tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag
      721 agtgaaagaa gctcaatact ctttctcaaa atgagaactc cggcagtagg actcgaacct
      781 acgacatcat gattaacagt catgcgctac taccaactga gctatgccgg aataacatca
      841 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
      901 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
      961 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatt
//
LOCUS       DASJRQ010000248         1002 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJRQ010000248 DASJRQ010000000
VERSION     DASJRQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.258"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1002
                     /locus_tag="U4A80_002900"
                     /pseudo
     CDS             369..1002
                     /locus_tag="U4A80_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DASJRQ010000249          982 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJRQ010000249 DASJRQ010000000
VERSION     DASJRQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.259"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..590
                     /locus_tag="U4A80_002901"
     CDS             39..590
                     /locus_tag="U4A80_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HEN1976467.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          376 a          136 c          188 g          282 t
ORIGIN      
        1 agagcgagca agacaggaac gagagaacaa acatactcat gaaaagacta gaggcttcga
       61 cctcgaactt taataataag ttagatgttt ttgaagaaaa gactaagcat gcaagattaa
      121 actttgaaac aactgcaaag cactatatta aacgacttga tgaagataat ttgaaattag
      181 attttcaaca agctattcaa gatgaattaa gcgatactaa agacgaaata cgtgaagtga
      241 cgaaacaagc gagagaagaa acaaaagagt ataaagaaat cttagaaagt aaaataaaag
      301 accataacaa agtggttgat aaatcgaata cagcgctaaa agtgatgaca aaaggcgtta
      361 caaatatatt ttttgtgcta attatttttg tacttgtaat gcttgtgaca ggtccgatag
      421 gtcatttctt tggtattgaa catttatata gctttattaa tggttttatt gatgaccacg
      481 aaagtgcatg gcgatatttg atgttgatac tttatgcagt accctatgta ttcttcgcta
      541 tcattctgtg gctcatagca ttgtttttta gagtatttaa tgacctttag gtagatttat
      601 atataaaaag ggctaaatac gcaaaatacc cccttaaaat gcaaaataaa tgcaaataag
      661 ggggtaactc cttatgaaaa aacaactaat agtattataa cacgttttta tgccgagaaa
      721 acttattggt tggaatgggc tatgtgttag ctaacttgtt agcgagttgg ttggacttga
      781 attgggatta atcccaagaa agtaccaact caacaacaca taaagccctg taggttccga
      841 ccaataagga aattggaata aagcaacgaa aggagttgag taaaatgagt gagaaaacga
      901 agaaaatcat tcgagtatca ttagcttttt cacaagtagt agtgcaatca ttgatagttt
      961 attatactta ccagcaatat aa
//
LOCUS       DASJRQ010000250          968 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJRQ010000250 DASJRQ010000000
VERSION     DASJRQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.260"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>968
                     /locus_tag="U4A80_002902"
     CDS             <1..>968
                     /locus_tag="U4A80_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1976468.1"
                     /translation="LSWGDKVSISDYAENLSITPDLSNVPLHKENTYTVKELFDSAII
                     QSANASMVALAEKISGSEAKFTERMKEQLKDWGIKDATIVNASGLNNSYLGENRPEGT
                     GENDENQMSAQDVAIVARHLILDFPEILDVSSTTTQMFGENTQSPVEMVNWNWMLPGF
                     VNYKEGVDGLKTGTTELAGACFVGTITKNDQRIITVVLNADGHAENPSARFNETDRLM
                     DYCYDNWSEKELGKANASIPSLKTIDVKDGKESTVPVVLKSPVKVWVRNDMDTGKLTI
                     TPTLDKKQVTDNEIQAPMDKGTKIGTATITLADDKLGYLEDNNAPS"
BASE COUNT          334 a          199 c          211 g          224 t
ORIGIN      
        1 actgtcatgg ggtgataaag tttcgatttc agattatgca gaaaatttga gtatcacccc
       61 agatttatcg aatgttcctt tgcataaaga aaacacctac actgtcaaag aattattcga
      121 ctctgcaatc atccagtcag ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg
      181 cagcgaagct aaattcactg aacgaatgaa agaacaattg aaagattggg gaatcaaaga
      241 tgcaacaatc gttaatgcat caggattgaa caactcttat cttggcgaaa accgaccaga
      301 aggcactgga gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg
      361 ccatttgatc ttggatttcc cagaaatact ggatgtatca agtactacga cccaaatgtt
      421 tggcgaaaat acgcaatcac cagtggaaat ggtcaactgg aactggatgc tcccaggatt
      481 cgttaattac aaggaaggcg tggacggatt aaaaactggt acgactgagc ttgcaggtgc
      541 ttgtttcgtt ggtacgatca ctaaaaacga tcaacggatc atcactgtcg tattgaatgc
      601 ggacggccat gcggaaaacc cgagtgcccg cttcaatgaa acagaccgtt tgatggacta
      661 ctgctatgac aattggtcag aaaaagagct cggaaaagct aacgcaagta ttcctagtct
      721 aaaaaccatt gatgtaaaag acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc
      781 tgtaaaagta tgggtacgaa acgatatgga tactggcaaa ttaacgatca cgccaacact
      841 tgacaagaag caagtcactg acaatgagat ccaagcacct atggataaag gtacaaaaat
      901 cggtaccgct acgatcacct tggcagacga taaattaggc tatttagaag acaacaatgc
      961 accatcaa
//
LOCUS       DASJRQ010000251          959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJRQ010000251 DASJRQ010000000
VERSION     DASJRQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.261"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..511)
                     /locus_tag="U4A80_002903"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="U4A80_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="U4A80_002904"
     CDS             complement(570..>959)
                     /locus_tag="U4A80_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976469.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DASJRQ010000252          957 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJRQ010000252 DASJRQ010000000
VERSION     DASJRQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.262"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..333)
                     /locus_tag="U4A80_002905"
     CDS             complement(<1..333)
                     /locus_tag="U4A80_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1976470.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
     gene            complement(463..>957)
                     /locus_tag="U4A80_002906"
     CDS             complement(463..>957)
                     /locus_tag="U4A80_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEN1976471.1"
                     /translation="GIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGI
                     SKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASS
                     QLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELL
                     IDNN"
BASE COUNT          314 a          178 c          136 g          329 t
ORIGIN      
        1 gatgtcggag acatctcgtt ttctattagg aatcaactca ggttcaaaag aagcctcccg
       61 gtctctggga acatcaatgt ttactttacc aaagctcgtt ttaagcgtct tattgccata
      121 accgtttcgt cgattgtcat gtttctttgg ctctttagaa tgggcgtcat accctaaatg
      181 gttattcaat tctccttgaa gcattttttc aaataaggga ccaaacacat cttttaaaac
      241 atcttgcata tcctctacag attcgggttg ataagtattc aaaatggatt cagctaactt
      301 ctcagcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg atcttatttt
      361 agaaaaataa aaaccgtgaa gcaaccaccc gcctaggatt gatggttact tacacggttt
      421 atattacact ctcatttttg gtgctaaaaa gcaccgaaaa ttttaattat tgtcaattaa
      481 taattctttt ggattccttc ttaatatatt gaatgaagaa ataatcaatg atacaagaag
      541 tactaaactc ataaatataa ctacataaat catgaattta ggtaatacat tgatatctaa
      601 acttgataat gtcttgttaa atccttcagc ttctgcccca ccacctaatt ggctagatgc
      661 agattgttta gctatttgtt tagctatatc accagtaacc ttattcaata tattattccc
      721 aagcttgtca gctgtatatt gagctaagaa ataggaacca acgaatgcgg ggattgatat
      781 aaataccatc tcaattagga attgaccaaa aatttctaat tttgatatac ctaatgatag
      841 taatactgct atttctttct ttctagcatt catccataag aataataata atgaaactac
      901 aactcctgca aatattaatg agccaaaaaa taatttattt gaaattgagt aaatacc
//
LOCUS       DASJRQ010000253          947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJRQ010000253 DASJRQ010000000
VERSION     DASJRQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.263"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            337..678
                     /locus_tag="U4A80_002907"
     CDS             337..678
                     /locus_tag="U4A80_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1976472.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            697..>947
                     /locus_tag="U4A80_002908"
     CDS             697..>947
                     /locus_tag="U4A80_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976473.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIINE"
BASE COUNT          349 a          118 c          210 g          270 t
ORIGIN      
        1 cctagattaa tcatgagcag ctactaaggg atctaggctt tgacgcaaac tcatggaata
       61 aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta
      121 catgtagata cttgcgagga cgttggtcat tggatcaatc gagcatttac cgctgtctac
      181 tagggtttct cgtttcggac gccataaacg agtcgatatt ttttatgtat ttttaagtaa
      241 acgaaaaacg ataaaatatg aacgagtcct gaataaagtg aaaaaattga tataaaacat
      301 agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata
      361 cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa aggttttgtg
      421 acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaaa cgaggatgat
      481 ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat
      541 gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt
      601 gaagatactt ggtcatattt gagtgattat attgtcagcg ttgaaatcat tgaatttgta
      661 gcggataaag aagaataaaa actggaggaa taaattatga aatttttcga agtaaaatca
      721 ccatactatt atgcgttaat cgcagcaaag aatgaagaag aatgtgcaga attgtatgaa
      781 caagtagtaa cagatatcga aaatcgtgaa gaatttattc gatgtttgca tgagttaaat
      841 cgaaatgaag caattcaaga ggatgcgaaa aaaattagtg aagaaacgtt acaaccaatc
      901 ggtatggagg aagctacaaa agacattttt acaattatta atgaaca
//
LOCUS       DASJRQ010000254          942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJRQ010000254 DASJRQ010000000
VERSION     DASJRQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.264"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            208..909
                     /locus_tag="U4A80_002909"
     CDS             208..909
                     /locus_tag="U4A80_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1976474.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          338 a          162 c          150 g          292 t
ORIGIN      
        1 tttactatat gtattattgt tgttttttat acacaaatca ttcgcttcaa aacaaattat
       61 tcaataccat tgtatatttt ttacataata acaaattata tttgtaaagt attaaaactt
      121 gtttctaatg ttggtatgtt aaaactttac aaattcttta aaaaaagtga caataaatat
      181 agtgaacaat aaggagagga ctttttaatg cgtgttaata aaatcgattt tcacttttca
      241 aaactcagat atcaacctga ttttgaacat ttctcggatg aagagttcca acagatcaag
      301 gaacatactg ttcttcgttc ttataaaaaa ggacagatcc ttttcgacga tggtgatccc
      361 agacaacggt tgtatatctt gaccaaagga ctcgttcgtt tagaacgtta tgatgaaagt
      421 gcaacttatt actactatga ctatgtgaat acaaataacc tttttcctat gtcaggaatg
      481 tttggagaag agacctacac ttatacagct caagcgatga ctgatattga aacattctac
      541 cttcctgttt cctactatga aaaattagca cgaaaaaatc ccgaacagtt gatctatatg
      601 atacaaaaaa tttctaaagt agttgaaata catgaattgc gtatccaaat cggattgacc
      661 tctagcgcct ttgaccgggt aaaacaaaat ttattcattt tgaaagaaga attgggcgta
      721 ccaacaaaag atggtcatat caccattcct tatccgatca ccttaaaaga attagcagtg
      781 aatagcggca cgacacgtga aactgctgga caagtaatca aacaactacg tgattccggg
      841 ctgttagatt atgacaaaaa gcgttttact ttctgtaaga cagctactac tccagctatg
      901 agagaataac gcgaacaaaa ataagcagtg accctgataa at
//
LOCUS       DASJRQ010000255          927 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJRQ010000255 DASJRQ010000000
VERSION     DASJRQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.265"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>927
                     /locus_tag="U4A80_002910"
     CDS             <1..>927
                     /locus_tag="U4A80_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEN1976475.1"
                     /translation="NQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLI
                     GFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINS
                     TADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYG
                     NTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGP
                     ILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVG
                     KDIFIKHYHIVVESWGLLFVQAIIEIYFKNYV"
BASE COUNT          296 a          115 c          178 g          338 t
ORIGIN      
        1 gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa gttaaagcta tgggcatagc
       61 aggtattatc agcacaattg taacaataat tgccaatatt ttcttcttgc ttattctgaa
      121 atggggatta attggttttt tcattgcaaa tattttatca cagattattt cagctactta
      181 tctgtcagta ttagtgagaa tatgggaata tatcgatttt gaggctaaag atcctcagct
      241 aaagagagaa atgctaaatt actcgattcc attaattgca actgttgttg gatggtggat
      301 taatagtaca gctgataggt atgtggtttc ctttatagta ggtgtctcgg cgaatggctt
      361 attgtctgtg gcatataaaa tacctcaaat aattaatact ttgcagggaa tatttataca
      421 ggcctggcaa atttctgcaa ttaaagaata tggagataat gagactgcaa aattttatgg
      481 aaatacattt gttatgatta atttgttaat gtgcataacc tgttcaagtc ttatcatatt
      541 aactagaccg ttttcacata ttctttatgc gaaagacttt tatgaagcgt ggaaatatgt
      601 tccattttta ttaatagcta atgttttaaa ttgtgcatca ggtctgttag gacctatctt
      661 atcagcaaaa aaagattcta aatccatgat gtggtctgca atcatagggg cattagttaa
      721 tgtgattatg agtgtagtcc ttgtttataa gataggggtt caaggtgcca caatagccac
      781 tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga gctgtgggaa aagatatttt
      841 tataaagcat tatcatattg ttgtggaatc ttggggatta ttgtttgttc aggcaatcat
      901 agaaatatat tttaaaaatt atgtaat
//
LOCUS       DASJRQ010000256          913 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJRQ010000256 DASJRQ010000000
VERSION     DASJRQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.266"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..490)
                     /locus_tag="U4A80_002911"
     CDS             complement(<1..490)
                     /locus_tag="U4A80_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1976476.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFD"
     gene            complement(566..712)
                     /locus_tag="U4A80_002912"
     CDS             complement(566..712)
                     /locus_tag="U4A80_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1976477.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(816..>913)
                     /locus_tag="U4A80_002913"
     CDS             complement(816..>913)
                     /locus_tag="U4A80_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1976478.1"
                     /translation="QEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          349 a          150 c           96 g          318 t
ORIGIN      
        1 gatcaaatac ttgatattga tgagcaacat aagaaacaaa taaaccaatt gtaagtaaat
       61 aaatcatgaa tatagcagta agtttagaac tggactgttc aacaatccta tttatagcta
      121 attttctata taaccaaata aacatcagca aaaacaaaga atcaagcatt atctggaata
      181 ctacataacc ataccctttt atcccttcaa atgaaaacat atacgtcata accatggaag
      241 taataataga tctgaaatca tcaataagca tacataatag taatttattt aaaagcacta
      301 tattagtttt cttcttttcc tttaatataa aataacttcc gattataggt atagctgcgg
      361 aaacatcagt aaataattca acaaaaaaag tgagtggtat caataaacat acaaaaagat
      421 attttctttt gttaagatca gtattagtca tataaaataa gagaaaaaag aaaaagtttc
      481 ccacgaacat tcttaataga taagcttttg tatcgatagt aatcaagaga tcccctcctg
      541 attttaggat atcataaaaa aaattttagt tacacttttt aaataatgaa aaaacacatt
      601 ccactaaatt tgatgccggt tttccttgag gttttgttcc agcacctcct ttaatttttt
      661 taagctcttg atctgaacat tgtttagcat ttagtctatt tttttcttcc attacgattc
      721 cccctacaaa ctataaatac ttttatgtca ctgattcaat atacctattt ttctaatcaa
      781 aaaattttga gacatattta atattataag tatcattaaa attgagattt atctccataa
      841 tctgctcgat ataaaccatt tattccagca gttcttccaa tttcactcaa atttcttatt
      901 aattcctctt gat
//
LOCUS       DASJRQ010000257          898 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJRQ010000257 DASJRQ010000000
VERSION     DASJRQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.267"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..>898
                     /gene="bsh"
                     /locus_tag="U4A80_002914"
     CDS             5..>898
                     /gene="bsh"
                     /locus_tag="U4A80_002914"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1976479.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAID"
BASE COUNT          305 a          122 c          164 g          307 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaagcta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgacgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gtaggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgat
//
LOCUS       DASJRQ010000258          865 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJRQ010000258 DASJRQ010000000
VERSION     DASJRQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.269"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..109
                     /locus_tag="U4A80_002915"
                     /pseudo
     CDS             1..109
                     /locus_tag="U4A80_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            184..>865
                     /locus_tag="U4A80_002916"
     CDS             184..>865
                     /locus_tag="U4A80_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HEN1976480.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          290 a          157 c          176 g          242 t
ORIGIN      
        1 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
       61 actagaccac agcttacttg gaaaacaaag aaaaacagtt gagactgttt cagactgtag
      121 acaaagtcca ttttatggat gatgtctaca gtcttttctt ttataataga tttatgaaaa
      181 cgaatgcaag gagtgatagc tatgatgtct aaaaacaata cgaatggtag aaatcaattt
      241 gcgatgctta cgattgatga tctagttcct caagatcatc tcgttcgaaa gattgatgcc
      301 gcgcttgatt ttgaatttat ttatccaatc gtcgaagcca cttactccga tttaggtcgc
      361 ccaagtatcg atccagtgat tctgatcaaa ttggtgttta ttcagtattt attcggtatc
      421 cgttccatgc gacaaaccat taaggaagtc gataccaatg tcgcttaccg ctggttttta
      481 ggctactcct tcgaggagaa aatcccccat ttctcaactt ttggaaagaa ttatgttcgc
      541 cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga acaagcagtt
      601 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
      661 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      721 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      781 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      841 ggctactacg tcaaaggcga acgag
//
LOCUS       DASJRQ010000259          850 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJRQ010000259 DASJRQ010000000
VERSION     DASJRQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.271"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          321 a           98 c          110 g          321 t
ORIGIN      
        1 attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg aaaagtgaaa
       61 agagcaaata taaaatttaa taaaaatata gaactaaata ggaaaacgta gacgtaattt
      121 tctacgtttt ccttttcata tatattaaat attacggaaa attatataaa ctaatttagt
      181 gttttgtttg taattcctaa ttttgctcca cttttagata atttttaggt ctatttaact
      241 catcagacat tctatgctca tttttctcca aatttgtttc aactctttcg tacttaattt
      301 tgcgtatttg aaaattcttc ttttagagta cttttattac gatactagta tgatatatat
      361 ctatagcaaa cagttaaaat cttcctaaaa taagcgaata gcattcctaa aacctataaa
      421 atttttagat gaagcgacaa gtggacaatt aataaactac ttgaacatga aattatggat
      481 cacctttcaa atttagaaaa tggaattctt atacttattg cgcaacattt attgattaca
      541 aaattatgtg acaaaaagaa ctagtagaag aaggaataat ggcttgataa taaagtacta
      601 cctattaaag gtagtacttt atttaattta gtataaatta acatactact atattttagg
      661 tttcatatta tataaattaa taataaaata ccatttgttg gtagtataat ttaaaggtaa
      721 taagttattc ttttattact atgtttgggt tttatagcaa ggaaagttta ataagagttt
      781 ggacctagca aacaatatta atgaggtcat taaatcttat tatctttggt tctgttgcaa
      841 agttttaaat
//
LOCUS       DASJRQ010000260          844 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJRQ010000260 DASJRQ010000000
VERSION     DASJRQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.272"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..503
                     /locus_tag="U4A80_002917"
     CDS             <1..503
                     /locus_tag="U4A80_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1976481.1"
                     /translation="LAELYDDDYSKDTHFSNLLNEIKENYDFIIIDVPPQLNKFTNSA
                     LVASDYVIVILQTQERSLKGAEKYIEHLIQLNNDYGTEIDILGLLPVLVQNGNDLDLD
                     IIEDAESLFGKSNIFNTKIKTMQRLKRYDRTGITDNPKDIHDKRVQQVYQSVSDEVIQ
                     RINLLG"
     gene            517..>844
                     /locus_tag="U4A80_002918"
     CDS             517..>844
                     /locus_tag="U4A80_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976482.1"
                     /translation="MTNEKNESIGLNAIKRKNKTQRPENIVPENSEALNNTTDSGKIL
                     EQSKKRLTTKDLPKSIRMPLDTHTAISTIATIEDKKIYETLNKIVEFYINNMNPANKK
                     IVKNSIK"
BASE COUNT          370 a          120 c          122 g          232 t
ORIGIN      
        1 atttagcgga attatatgac gatgattatt ctaaagatac gcatttctca aatttattaa
       61 atgaaattaa agagaactat gattttatta tcattgacgt accaccacaa ttaaataaat
      121 ttactaattc tgctttagtc gcaagtgact atgttatcgt aattctgcaa acacaggaac
      181 gctcgttaaa aggagctgaa aaatatatag aacatttaat tcaattaaac aatgattatg
      241 gaacagaaat agatatttta ggcttgctac cggtactagt tcaaaacggt aatgatttag
      301 acctcgatat tattgaagac gcagagtcat tatttggaaa atcgaatatt ttcaatacaa
      361 aaatcaaaac aatgcagaga ttgaaaaggt atgatagaac tggcataacc gataacccaa
      421 aagacattca tgataaaaga gtccaacaag tttatcaaag tgttagtgac gaagtaatac
      481 aacgtattaa tcttttagga taaggagctt ttaaaaatga ctaatgaaaa aaatgaatca
      541 attggtttaa atgccataaa aagaaaaaat aaaacacaac gacctgaaaa tattgttcca
      601 gaaaattctg aagccttgaa taatacaact gattcaggca aaattctaga acaatccaaa
      661 aaacgactca caactaaaga tttacctaaa tctataagaa tgccactaga tactcacact
      721 gcaatcagca cgattgcgac aattgaagat aaaaaaatat atgaaacgtt aaataaaatt
      781 gtagaatttt atataaacaa catgaatcca gcaaataaaa aaattgtaaa aaatagtata
      841 aaag
//
LOCUS       DASJRQ010000261          842 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJRQ010000261 DASJRQ010000000
VERSION     DASJRQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.273"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>842
                     /locus_tag="U4A80_002919"
     CDS             <1..>842
                     /locus_tag="U4A80_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN1976483.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLK"
BASE COUNT          367 a          108 c          136 g          231 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 tt
//
LOCUS       DASJRQ010000262          839 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJRQ010000262 DASJRQ010000000
VERSION     DASJRQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.274"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..718
                     /locus_tag="U4A80_002920"
     CDS             2..718
                     /locus_tag="U4A80_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1976484.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          292 a          160 c          166 g          221 t
ORIGIN      
        1 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
       61 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
      121 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
      181 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
      241 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
      301 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
      361 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
      421 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
      481 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
      541 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
      601 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
      661 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
      721 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
      781 gtcttttatt ttttggctct atactttttg atgttggtga acttcaccaa catcttttt
//
LOCUS       DASJRQ010000263          834 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJRQ010000263 DASJRQ010000000
VERSION     DASJRQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.275"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..566
                     /locus_tag="U4A80_002921"
     CDS             <1..566
                     /locus_tag="U4A80_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEN1976485.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT          267 a          111 c          178 g          278 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgt
//
LOCUS       DASJRQ010000264          822 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJRQ010000264 DASJRQ010000000
VERSION     DASJRQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.276"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..458)
                     /locus_tag="U4A80_002922"
     CDS             complement(39..458)
                     /locus_tag="U4A80_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976486.1"
                     /translation="MKKSHIVTLATIFLLCVAAFFFFTNSPKNNKHITARTATTESTI
                     KTETVYPSLNELKQSQGSDIEILGKTQITTVLFQNKNMAILTTENNKEIQLPLIVTDK
                     KNNLFEIPSLTYSKKNLKVGEVFGLAKKDNHYLAYKL"
     gene            complement(504..746)
                     /locus_tag="U4A80_002923"
     CDS             complement(504..746)
                     /locus_tag="U4A80_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976487.1"
                     /translation="MNITNIEELKEWIETNLVTKQEAIKITEQSPKGFQQSVRTNVIK
                     PFYESKEGKGPSVVRLYLKSDIETYAKQLKEKKQKP"
BASE COUNT          243 a          116 c          139 g          324 t
ORIGIN      
        1 aatgaggtgc aaaaatagag agacaaatgt ctccctattt ataatttgta tgctagatag
       61 tgattatctt ttttagctaa accgaagact tcaccaactt ttagattttt ctttgagtaa
      121 gtgaggcttg gtatttcaaa caagttgttt ttcttatcag tcacaattaa gggaagctgt
      181 atttctttgt tgttctctgt agttagaata gccatgttct tattttgaaa taaaacagta
      241 gtaatttgag tcttccctag aatttctata tctgaacctt gcgattgttt tagttcattc
      301 aaagaaggat agacagtttc tgtttttatt gtactttctg tggttgctgt cctcgctgtg
      361 atatgtttat tgtttttggg cgagttggta aaaaagaaaa atgcagcaac gcaaagtaag
      421 aaaattgttg ctaatgtaac gatgtgtgat tttttcatag catatctcca ttaatatcaa
      481 tttataaaaa gcagaaactg taattatggt ttctgctttt tctcttttaa ctgtttagca
      541 taggtttcaa tatcgctttt tagatagaga cgaactactg aaggaccttt tccttccttg
      601 ctctcataaa atggtttgat tacatttgtg cgaacagact gctgaaatcc tttaggagat
      661 tgttcagtaa tcttgattgc ttcttgttta gtaactaaat tagtttcaat ccattctttt
      721 agttcttcta tattagtgat gttcatatta atcccacctt tctgtggttt tgattgtcta
      781 cagaaatatt ataaatgaaa aaaagtaaaa aatctaatat aa
//
LOCUS       DASJRQ010000265          816 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJRQ010000265 DASJRQ010000000
VERSION     DASJRQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.277"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>816)
                     /locus_tag="U4A80_002924"
     rRNA            complement(<1..>816)
                     /locus_tag="U4A80_002924"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          165 a          259 c          181 g          211 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DASJRQ010000266          801 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJRQ010000266 DASJRQ010000000
VERSION     DASJRQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.278"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..466
                     /locus_tag="U4A80_002925"
     CDS             44..466
                     /locus_tag="U4A80_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976488.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            476..679
                     /locus_tag="U4A80_002926"
     CDS             476..679
                     /locus_tag="U4A80_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976489.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          308 a          116 c          123 g          254 t
ORIGIN      
        1 ttaagaaaac gttttcttat ttttgatttc gaggtgatag acaatgaaaa ataagtttat
       61 tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt
      121 tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat taactccttt
      181 agataggaac ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta
      241 tttaatagtt tctccattac ttgctcgagc agcttctaaa acgatacgag tgacaggaac
      301 gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga
      361 aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata aatcagctgg
      421 aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc
      481 agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta aaattaattt
      541 accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca
      601 aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac
      661 atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat tctttttttc
      721 taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt gctttatttt
      781 tcaaaaaaac aattcatttc a
//
LOCUS       DASJRQ010000267          792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJRQ010000267 DASJRQ010000000
VERSION     DASJRQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.279"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            67..705
                     /locus_tag="U4A80_002927"
     CDS             67..705
                     /locus_tag="U4A80_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1976490.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          244 a          130 c          127 g          291 t
ORIGIN      
        1 tgcctatttt aactcttcat gtatactgac tctatcttca aaaactgaat agtaggagtt
       61 attatcatga ataacatttt aggaaaaaaa atagaactta aaaaagggat attgggcact
      121 attgtcgttg tactcggttt gttagggttg ctatttgcgc catccattat tagcattttt
      181 tctttaacta tcactggatt tatagcattt acacttgttt ttggtaaaaa agatacgaaa
      241 aaaatgttct cgaaacctgt cgctcctgtc aaaaatatag ccaaatattt ttttatcaat
      301 gtagtaatca gtgccgcagt ctcggtattt ttgcaacaaa ttttgaaatg gggtttaaca
      361 ggcaatccaa taaatgaggc tttcagcccg ttattattta ttattttgcc tatcatgata
      421 cttggagaag aattgttctc agtgtatttt ctcgctattt tttctagtaa gttcagcatt
      481 cctgtcgcta gtttcttgtc agcaatcatc tttggattta tccattacag tacttatgat
      541 aatggaaata ttttacacac tgtagcacac atccttttga tacaaggggt ggcaagattg
      601 ttattcaacc aagctgccat taaaagcaac tcgatcatta caagttgggc agttcatgtg
      661 atatttgatt tcacagctat attgctagtc gttttattct cctaaatttt tatagaaata
      721 atacatgctc tattgctaag aaagcatact ttatttcctt taaacaaaaa gacagatgta
      781 tcaatggatt aa
//
LOCUS       DASJRQ010000268          792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJRQ010000268 DASJRQ010000000
VERSION     DASJRQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.280"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..303
                     /locus_tag="U4A80_002928"
     CDS             <1..303
                     /locus_tag="U4A80_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976491.1"
                     /translation="FLCMSYEPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLK
                     DTGVLIGTNSKVYQEVKEKIENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT          304 a          151 c          126 g          211 t
ORIGIN      
        1 tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa ccaagcaaaa
       61 ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga atttgctgat
      121 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata tcaagaagtg
      181 aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa aaatcgtcaa
      241 aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt tctaaaaaga
      301 tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg gcagtcttcc
      361 agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc ttgtgcgaat
      421 ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat ttaatagcgt
      481 ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta acgagcagcc
      541 aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc gcttatgaag cgctgccaaa
      601 aatttaccat attttatgaa aaataacaag tttctaaaaa ggaatcggtt aagattcaca
      661 aagaatcgta aacagacaat agcggaaaat caagcttccg aacgtacctt ttagctagac
      721 caatgaaaaa tcacaaagtg agcccctgta tcggttgata caagggctac ttggcatact
      781 cgacattatg tc
//
LOCUS       DASJRQ010000269          769 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJRQ010000269 DASJRQ010000000
VERSION     DASJRQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.281"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            40..711
                     /locus_tag="U4A80_002929"
     CDS             40..711
                     /locus_tag="U4A80_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1976492.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          305 a          116 c          120 g          228 t
ORIGIN      
        1 taaaatatca gttaaaataa aagttaagga gcattgtgta tgacaattaa aactagtctc
       61 caaaatcaag cttaccaatc tattcgccga caaattatct atgctgactt agaacctgga
      121 aaaaaagtat cagaaaaaaa attagaagaa acactttcta ttggtcgtac accaatacgt
      181 gaagcactga ttcaactccg acaacaaggt cttgttgata caattcctca aagcggaaat
      241 tatatctcat taattgattt agattcggcc aaaaatgctc gatttgtcag agaacatttg
      301 gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata accaaaaaaa acggatttta
      361 gaaactataa tttcagaaca agaaaaagct gttagtcaaa aaaatgctcg tagttttttt
      421 cataatgata atttattcca taaagcttgt tttgaaattg cagagcgaga agaagtatgg
      481 caatggttag aaaataacaa tacacactta gaacgatttc gatggctacg agtaaaaacg
      541 gaaggattaa agtgggaaaa aattatggaa caacattatc aactatttga ggcattaaca
      601 aaacaagatc cagaagaagc taactttcta acatcgttgc atcttcattt aatgcttaat
      661 gagcaaaata ttgttttagc tactcatcca aaatatttca aaaatatata acttttaact
      721 ctttttaaat taaatggttg ttctgatcag aaaaatatcg cttgttttc
//
LOCUS       DASJRQ010000270          762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJRQ010000270 DASJRQ010000000
VERSION     DASJRQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.282"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>762
                     /locus_tag="U4A80_002930"
     CDS             <1..>762
                     /locus_tag="U4A80_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1976493.1"
                     /translation="SLISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGN
                     VKGEDIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLT
                     GVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFD
                     ADNEKGADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVY
                     QDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSS"
BASE COUNT          329 a           83 c          144 g          206 t
ORIGIN      
        1 agtctcatta gtttatctat taaagatgct acggacagag cttcaaaaga aacatttgct
       61 aatataacaa atagtttttc tatggagata aatagacaag taaatccagg aacacctaga
      121 ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga tagtatagac
      181 tcctatgtaa aaagaataaa cagtgttgca gatttagttg attatgatat tattgaaact
      241 caagagactc ttgcgaatca atcacctgaa agagcaaaga attttaaaag aacagttatg
      301 ttaactgggg ttaacgactc aacaaaagaa acaaaatttg tatcagaagc atataaatta
      361 gtagaaggaa agcatttaga aaatgaagat aaaaataaaa tcttaatgca taaagattta
      421 gctgaaaaaa ataaccttaa agttggagat aagataaaaa taaaatctaa tttatttgat
      481 gctgataatg agaaaggtgc agatgaaaca gtagaagtag aaattaaagg tctattcgat
      541 gggcataata gcggtggagt aagtgcagca caagaactat acgaaaatac attaatcact
      601 gatattcata cagctgctaa agtttatggt aataccgaag atacagctgt ataccaagat
      661 gcaacattct ttgtaaaagg tgataagaat cttgatagtg taataaaaga tcttgggaaa
      721 ttagatataa attggagaga atataatttg attaaaagtt ca
//
LOCUS       DASJRQ010000271          759 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJRQ010000271 DASJRQ010000000
VERSION     DASJRQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.283"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..93
                     /locus_tag="U4A80_002931"
     CDS             <1..93
                     /locus_tag="U4A80_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1976494.1"
                     /translation="AGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            105..>759
                     /locus_tag="U4A80_002932"
     CDS             105..>759
                     /locus_tag="U4A80_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1976495.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     "
BASE COUNT          252 a          178 c          163 g          166 t
ORIGIN      
        1 gctggtatcc gtaaaaaagg aacagctcct gcacgaccaa aagctacaaa cgataaaaca
       61 gccccatcac gcagaagccg ctcaagagtc taggaggaag aaaaatggca cgaacacaga
      121 aacaaaaagc agcacaaact catgcagtca gtttgatcac attaacaaac ccatcttcac
      181 caatcgccga acaatatcgt acgatccgga cgaatatcca gtttgcctcc gcagctggtc
      241 agcaaatcaa aacgattgtc gtgacttcat caggtcctgg agaagggaaa tccaccactg
      301 cagcaaacat tgcagtcgtt tttgctaaat caggtcaacg agttctacta gtcgatgcag
      361 atttacgtaa acccgtcgtc tataagacat tccagctgaa caatgccagt ggattaagta
      421 cagcacttag ttcatcagga agtgtagcag atgtgatcca acgaacgcca gtcgagaact
      481 tgtcgatttt gcctagtgga cctaagccac ccaacccatc agaattgtta agctcgccaa
      541 gaatggatca gatcctatcc gaagcaagac agttatttga cgtggtgata ttcgatatgc
      601 caccagtagt agccgtaaca gatgcacaga tcatgtcttt aaaaacggat ggcactttat
      661 tggtcgtacg tgaaaatatg tcacgtaaag aatccttgac taaagcaaaa gaactgctgg
      721 agatggtaca agcacgagtc ttaggtgttg tgtacaacg
//
LOCUS       DASJRQ010000272          748 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJRQ010000272 DASJRQ010000000
VERSION     DASJRQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.284"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..591
                     /locus_tag="U4A80_002933"
     CDS             <1..591
                     /locus_tag="U4A80_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1976496.1"
                     /translation="FDYLSWAHAWEIMKKNDPQATVTINEYKHYRVVSGTHQDFLVEE
                     YKPFLMDETGTYVSVSVTVKGHTETELFPVLDYRNQPVVKPNAMQINNSLKRCFVKAL
                     ALHGLGLYVFQGEDIPTPPRIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDK
                     LGLLADNVETIEQLSYEQCALMERAIAAKRKELDKK"
     gene            597..>748
                     /locus_tag="U4A80_002934"
     CDS             597..>748
                     /locus_tag="U4A80_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976497.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIR"
BASE COUNT          266 a          119 c          160 g          203 t
ORIGIN      
        1 tttgattatt tatcttgggc tcacgcgtgg gagattatga aaaagaatga tccacaggca
       61 acggtaacta ttaatgagta caaacactac agggttgttt ctggaactca tcaagacttt
      121 cttgttgagg aatataaacc ttttcttatg gatgaaactg ggacttatgt atctgtctca
      181 gtaacggtta aaggacacac ggaaaccgaa ttatttcctg ttttagatta tcgaaaccaa
      241 ccagttgtta aaccaaatgc aatgcaaatc aataactcat tgaagcgatg ctttgtgaaa
      301 gcattggctc tacacggact gggattatat gtatttcaag gggaagatat tccaacacca
      361 cctagaatcg atacaaagaa attaaacatg ctagagacga ttctagaagc tttcaatgag
      421 cagatgggta aagatatgac caaaacctta atcgaatatg ttaatgagca gacagataaa
      481 ttagggctct tagctgataa cgttgaaact attgaacagt taagctatga gcaatgtgcc
      541 ttgatggagc gagcaatagc agctaagaga aaagaattag ataagaagtg atatgagtgt
      601 ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa gttcaaagga aaagacataa
      661 gcgcaaccat caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg
      721 atggtgatcg agtcattgaa attcgttt
//
LOCUS       DASJRQ010000273          739 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJRQ010000273 DASJRQ010000000
VERSION     DASJRQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.285"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>739
                     /locus_tag="U4A80_002935"
     CDS             <1..>739
                     /locus_tag="U4A80_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1976498.1"
                     /translation="GLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILAN
                     SDGNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVG
                     SVNIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSE
                     GLIFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIIT
                     SNNSLLTEVARPRLTSITQPLYDLGAVS"
BASE COUNT          249 a          137 c          156 g          197 t
ORIGIN      
        1 gtggcttggc aagtaagaaa acaacaacag ttggggtaat cattcctgat gtcagcaata
       61 tgttctttgc ctctttagca cgtggtatcg acgatgtggc aacaatgtac aaatacaata
      121 tcatcttagc aaactcagat ggcaacgatc aaaaagaagt caatgtattg aataatttat
      181 tggctaagca agtggacggc gtgatcttta tgggtcacca tatcacagac gaaatccgtg
      241 gagaattttc acgttcaaaa acgcctgttg tactagctgg ttccattgat ccagatgaac
      301 aagtaggtag cgtaaacatt gattatgtgt cagcaacaaa agatgcagta aataaattag
      361 caaaaagcgg caataaaaaa atcgcttttg tcagcggtgc gctgattgat cctatcaatg
      421 gtcaaaaccg tctaaaaggc tataaagaag cattaagcga aaacaatcta ccatatagcg
      481 aaggattgat ttttgaggca caatacaact ttaaagatgg tctgtctcta gttgaccgga
      541 tccataatag cggtgcaaca gctgtctatg tttcagacga tgaattagcc attggtatct
      601 tggatggttt attagaccgc ggcgtaaaag taccagaaga attcgaaatc atcacaagca
      661 acaattcttt actgacagaa gtagctcgtc ctcgtttgac aagtatcaca caaccattat
      721 acgatttagg agcggtatc
//
LOCUS       DASJRQ010000274          738 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJRQ010000274 DASJRQ010000000
VERSION     DASJRQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.286"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            390..>738
                     /locus_tag="U4A80_002936"
     CDS             390..>738
                     /locus_tag="U4A80_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1976499.1"
                     /translation="MKILIVDDEASILDIVEAYLSAKGYQVFRALSGNEALTKVDVVQ
                     PDLIVLDLMLPGLSGLEVCKKIRDSSTVPIIMLTAKTAEKDILEGLSLGADDYITKPF
                     SPKELVVRVETVLR"
BASE COUNT          228 a          135 c          170 g          205 t
ORIGIN      
        1 ctttccttta aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt
       61 atttgatagt agatttaaaa ctttgcaaca gaaccagaaa tgaatattgt aaaaaatgat
      121 taggtagcgt ttattaatcc atgttcataa cttaagactt tagacaacat cccaaaagca
      181 gaagattcta ctacgcctga caaagtgatg acctttagtg gcatgggaaa catggtcgcc
      241 attgacggca aacagttcga catggaccgg atcgatttac gcgctaaggt cggggaaaag
      301 gaagtttggg agattaaaaa cacacgggac atgatgggtc aagttttagt agaatgaaga
      361 taccaacgaa attggaggag aaagattacg tgaagatttt aattgtggac gacgaagcaa
      421 gcattctgga tatcgtggaa gcctatttga gtgcaaaagg ctatcaagtg tttcgcgctt
      481 tgagcggaaa cgaggcttta acaaaggttg atgttgtcca acctgatttg attgtgttgg
      541 atctcatgct gcctggttta tctggcctcg aagtctgtaa aaaaataagg gactcctcca
      601 ctgtaccgat tatcatgttg actgcgaaaa cagccgaaaa agatatcttg gaagggttaa
      661 gcctgggggc tgatgactac atcacaaaac cattcagccc gaaagagctg gttgttcggg
      721 tcgaaaccgt tttacgcc
//
LOCUS       DASJRQ010000275          738 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJRQ010000275 DASJRQ010000000
VERSION     DASJRQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.287"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          222 a          140 c          155 g          221 t
ORIGIN      
        1 tgttttgtag aaagggacct tatagtcact ttcacattac aaacgatacg tcctatggac
       61 gtatcaaaag ggtgggactt atgagcaaat taaagcaaaa tttggctagc aatccttcta
      121 aaaaatacac ctattgatca gagtcaaaat aaatcagttt tcgagtgagc gaatccaaat
      181 ttgcaactct gaagctttgc aaatgagtgt gccggcttcc gtaaaagtct aggtaacatt
      241 agtcgatata tacatagggc tctaatttgc gttttaaggc gttttaaacg ttctggtatg
      301 taattatacc aaagcgactt taaaacgcct taaaacgaat tttagaggtt ctgtagaacg
      361 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gctttaaaaa caagataagg
      421 gattttatct agacttgtct taaaacgccg tagatgagcg ctcagagctt ctaaaaggac
      481 tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga ctgcgcagtt taatgtgaga
      541 gcgtagcgat cacaccttta aagcgaccgt agggagcaga ttcccagttt aggaaatttc
      601 gcgaacctgt ttggagaaaa aaagtaggaa aattttaggc gggaaatttt gcccccctct
      661 tttataatct cttttataac ctctttttaa acctctttac cgtcggctgt atccttactc
      721 tcccaaggtg tgcagaat
//
LOCUS       DASJRQ010000276          737 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJRQ010000276 DASJRQ010000000
VERSION     DASJRQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.288"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..>737)
                     /locus_tag="U4A80_002937"
     CDS             complement(229..>737)
                     /locus_tag="U4A80_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976500.1"
                     /translation="CEKIFTDHMSGAKSNRPGLEMAIDFVRSGDTLVVWRLDRLGRNM
                     EDLISIVNRLNERGVSFHSIQENITMDKSSSTGQLMFHLFAAFAEFERNLILERSAAG
                     RAAARARGRFGGRPEKLSEQDLELLKTLVDSGTPIKTIAERWNVSRTTIYRYLAKMEE
                     KNGKTNSH"
BASE COUNT          242 a          161 c          106 g          228 t
ORIGIN      
        1 ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc
       61 aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc
      121 cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc atactattgt
      181 cacttttcta gataaatggc aaaaattttc agcattaaat gacaaaaact aatgagaatt
      241 tgtctttcca tttttctcct ccatttttgc caaataacga taaatggtgg ttcttgagac
      301 gttccaccgt tccgcaatag tcttaatagg tgtaccgcta tccactaaag tcttcagtaa
      361 ttccaaatct tgttcagaca atttttcagg acgaccacca aaccgtcctc ttgcacgagc
      421 agctgctcgt cctgctgcgg atcgttccag gatcaaatta cgctcaaatt ctgcaaatgc
      481 cgcaaacaaa tgaaacatga gctgtcctgt agaacttgat ttgtccatcg taatattttc
      541 ttgaatacta tgaaaactca caccacgctc attcagtcga ttgacgattg aaatcaaatc
      601 ttccatattt ctacctaaac gatctaagcg ccaaacgaca agtgtatcgc cagagcgaac
      661 aaaatcaatg gccatttcta atcctggtcg attacttttt gccccactca tatgatcagt
      721 aaaaattttt tcacaac
//
LOCUS       DASJRQ010000277          732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJRQ010000277 DASJRQ010000000
VERSION     DASJRQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.289"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>732
                     /locus_tag="U4A80_002938"
     CDS             <1..>732
                     /locus_tag="U4A80_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN1976501.1"
                     /translation="TIETKLKKNKEYERNNNVVVVGGAGSGKTRFYVLPNLLQMHSSY
                     VINDSKGGILSDTAAIMQQNGYRIKVFNLNTMKNSDGYNPFHYIRRTEGDCEIDILKV
                     VNNLMKNTSDPNKKGGDSFWDDAQRSFLAAIFHLLILKGKPEEQNLGIVADLVRRARV
                     KEDDEEYISSIDLVFQELEQEQPSCFAAKQYQNFKMAAGKTAKSILISLGVRLSPFDV
                     PDIRKLTSTDTLELDTIGDRKTVLY"
BASE COUNT          270 a           95 c          148 g          219 t
ORIGIN      
        1 aacgatcgaa actaagttaa aaaagaataa ggaatatgaa cgtaataata acgttgtagt
       61 tgtaggtgga gctggttcag gtaaaactag attttatgta ttgcccaatt tattacaaat
      121 gcactcaagc tatgttatta atgactccaa aggtggaatc ttgagtgata cagctgccat
      181 tatgcaacaa aatgggtatc ggataaaagt atttaatctg aataccatga aaaattctga
      241 tggttataat cccttccatt atattagaag aacagaagga gattgtgaaa ttgatatatt
      301 gaaagtagtt aacaatctaa tgaagaatac ttcagaccct aacaaaaagg gtggagattc
      361 attttgggac gatgcacaaa gatctttttt ggcagctatt ttccatttgt tgattttgaa
      421 aggaaaacct gaagaacaaa atttagggat agtagcagac ttagtaagac gcgcaagagt
      481 aaaagaagat gatgaagaat atatttcttc gattgattta gtatttcaag aattagagca
      541 agaacagcct agttgttttg cagcaaaaca gtatcagaat tttaagatgg cagcaggaaa
      601 gacggctaag agtattttaa ttagtttagg tgttcgtttg tctccttttg acgttccaga
      661 tatcagaaaa ttaacgtcaa cggacacctt agaattagat acaataggag atcgtaaaac
      721 tgtactttat at
//
LOCUS       DASJRQ010000278          722 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJRQ010000278 DASJRQ010000000
VERSION     DASJRQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.290"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..676
                     /locus_tag="U4A80_002939"
     CDS             59..676
                     /locus_tag="U4A80_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976502.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          260 a           96 c          140 g          226 t
ORIGIN      
        1 atttggagtt cagagagggc gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt
       61 gggttttcaa gtgctttttg gttcattaat cttaaccata gtgtttttaa agtggaaaac
      121 aagcaacaaa aaaagtatgt ggagccttgt aggaatagtt cttattattt ttactgtgac
      181 tgcagcaaca gaagcaaatt attcgcataa aattcagtcg tataaaggtc gtttagttat
      241 gaccaaaaaa gatgttacac gttatacatc ctcaagaact ggaggcctat atgactatgc
      301 accattagat gcattgaaat ataaagatca tttaaagaaa catgaagtaa aagttggtga
      361 tcggtgggag aatgcatctc ataaagcata taattccaaa atagtctata cagtagaaga
      421 tgccgatgag caaaatatca cattacctgt attcaattat tggggaacag ttgtgaaagt
      481 aaatgggaaa aagagcacta tagaaaatga caatggttta gtgtctatta aaggtacggg
      541 gaaaagaact atagttgagg tttcatctac gtatccaact acgatgattc tatctttatt
      601 aattagttca ataactttta tattcttatt tacacgttat ttaataagat gctgcggtga
      661 aatgaaaata gattagatag atattataaa tatgttgatt ctattttcat agcaatggag
      721 aa
//
LOCUS       DASJRQ010000279          692 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJRQ010000279 DASJRQ010000000
VERSION     DASJRQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.291"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..>692
                     /locus_tag="U4A80_002940"
     CDS             94..>692
                     /locus_tag="U4A80_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1976503.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT          233 a          125 c          145 g          189 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac at
//
LOCUS       DASJRQ010000280          691 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJRQ010000280 DASJRQ010000000
VERSION     DASJRQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.292"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>691)
                     /locus_tag="U4A80_002941"
     CDS             complement(<1..>691)
                     /locus_tag="U4A80_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976504.1"
                     /translation="IFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSV
                     SVVLMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYD
                     SIGTNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMS
                     FGDATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLL
                     EEAPGTYHHSMM"
BASE COUNT          233 a          141 c          140 g          177 t
ORIGIN      
        1 ccatcatgct atggtgatac gtacctggtg cttcttccag caactgtttc aataaaggat
       61 gattgggatt gctgagttca tttaatacga tcacactatc atcattgacc atcaattcaa
      121 tataggggtg cagacctatc gtcagcaaga atgaaaacag tgcacctgtg aatccgcaga
      181 ctagcgttaa ccaagtcgtt gcatccccaa agctcattcc ttggtagata atcaaaatga
      241 catccatcat cacaggaaaa ataatgaccc acatagcagc ggataaccct tgttctgaga
      301 tccgtttgcg tctgacgatc gttgccatga gaccagaaaa tacataggac atcaaaataa
      361 ctgtcagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcggat actgcttgaa
      421 aaataccagc catgatccct gctcttcgat tcaagaaaaa gcttaaaaca agtggagcaa
      481 aagccgcggg gtaaaacagc ggaatataag cagcttgttc tgtttggaag agttggaaaa
      541 atttcattag tacgacgctt actgacattg ccgtcacgta gaaaagtacg tattttgttc
      601 tttctcctgc attttggaat tgaagtgaat tataaatcag tacaccgatt tgaagcaaaa
      661 cagctaaaac catggcaatg agtgggaaaa t
//
LOCUS       DASJRQ010000281          690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJRQ010000281 DASJRQ010000000
VERSION     DASJRQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.293"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(80..634)
                     /locus_tag="U4A80_002942"
     CDS             complement(80..634)
                     /locus_tag="U4A80_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1976505.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          206 a          143 c           69 g          272 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca
//
LOCUS       DASJRQ010000282          665 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJRQ010000282 DASJRQ010000000
VERSION     DASJRQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.294"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="U4A80_002943"
                     /pseudo
     CDS             172..459
                     /locus_tag="U4A80_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..>665
                     /locus_tag="U4A80_002944"
     CDS             473..>665
                     /locus_tag="U4A80_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976506.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVD"
BASE COUNT          237 a           99 c          144 g          185 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgata
//
LOCUS       DASJRQ010000283          663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJRQ010000283 DASJRQ010000000
VERSION     DASJRQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.295"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>663)
                     /locus_tag="U4A80_002945"
     CDS             complement(<1..>663)
                     /locus_tag="U4A80_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1976507.1"
                     /translation="ANLLTLILAIFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYL
                     FIVFYNFLTVSFLYQFNRPRYNQDFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQS
                     RATLNPPILLMSGGQGADEKVPEAIAMKQYAMEQGIPERDILVETNSTTTLENMLYSK
                     EIMDQQMKGPYRAIFSSNNYHIFRAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLA
                     ILM"
BASE COUNT          209 a          124 c          144 g          186 t
ORIGIN      
        1 catcaagatg gcgaggtatt ctcttaaaaa cgcattaggc aagtagtaga aagctgtttt
       61 tgcaccaata ccatttgctt tcaatttggc ttgccgcgca taaagaccgg ctctaaaaat
      121 gtggtaattg ttggaactaa agatcgcgcg gtatggccct ttcatttgct gatccatgat
      181 ttcctttgaa tagagcatgt tttccaaagt ggttgtcgaa ttggtttcga caagaatgtc
      241 tctttcaggg attccttgtt ccatggcata ttgtttcatc gcaatggcct ctggtacttt
      301 ttcgtctgct ccttgcccgc cagacatcaa taagatgggc ggatttaatg tagcacggct
      361 ttgagcccga taaaaagcaa ttgccttatt gattcgcttg gctagaagag gagaaactgt
      421 ttctccattg atcaatccag cgcctaacac aacgatgaag tcttgattgt atcgaggccg
      481 gttgaactga tacaagaatg agacagtcaa aaagttgtag aatacgataa atagatagat
      541 caagatcagg ggaacgcaga aaaacaaagc atttatccat tgcggcaaat attttaacaa
      601 gaaaaagtca aaaacaagaa acagtgtaag aaaaatggct agtatcaacg ttaaaagatt
      661 agc
//
LOCUS       DASJRQ010000284          555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJRQ010000284 DASJRQ010000000
VERSION     DASJRQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.296"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            59..>555
                     /locus_tag="U4A80_002946"
     CDS             59..>555
                     /locus_tag="U4A80_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN1976508.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          203 a          103 c           91 g          158 t
ORIGIN      
        1 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
       61 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
      121 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
      181 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
      241 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
      301 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
      361 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
      421 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
      481 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
      541 tatggacgct actta
//
LOCUS       DASJRQ010000285          550 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJRQ010000285 DASJRQ010000000
VERSION     DASJRQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.297"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            125..523
                     /gene="spxA"
                     /locus_tag="U4A80_002947"
     CDS             125..523
                     /gene="spxA"
                     /locus_tag="U4A80_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1976509.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          157 t
ORIGIN      
        1 aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat
       61 gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg
      121 cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg
      181 gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag
      241 ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac
      301 acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt
      361 attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa
      421 acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg
      481 acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact
      541 tggaaaagac
//
LOCUS       DASJRQ010000286          546 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJRQ010000286 DASJRQ010000000
VERSION     DASJRQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.298"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(65..514)
                     /locus_tag="U4A80_002948"
                     /pseudo
     CDS             complement(65..514)
                     /locus_tag="U4A80_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          159 a          112 c           68 g          207 t
ORIGIN      
        1 agccataaca ccgcagcatt cacaacacct tataattttt gtcaaaaaaa gtgttgccat
       61 tccacttttt taaatatgcg ttctctgctc ttaaacgaat aacttcttct tcaagagact
      121 catctttaag ttttttagga atgtttagct tggaattcat actaattttt ctgccccttt
      181 tttggctctc aagtgaagaa gcaccgcctt cttcatattg ctctatccat ttacttagag
      241 ttctatttga accgatatta aattttttag cagtttcttg gatagaaaga ccatttgttt
      301 ccatatattc tataacatca agtttaaatt ttgtagtgta gcttttgcca cctccaacca
      361 agccttccca accatgatag ttataaatcc ttacccaatg tctaaccagt gtacgattta
      421 tttgatattt atgtgcaaga tatttgtagc cgccttcgtt atttaaatag tctgaaacta
      481 cttttttctt aaaaacaaat gtatatttcc gcaaaaaaag cacccctttt aattagattt
      541 ctagtc
//
LOCUS       DASJRQ010000287          546 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJRQ010000287 DASJRQ010000000
VERSION     DASJRQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.299"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..528
                     /locus_tag="U4A80_002949"
     CDS             <1..528
                     /locus_tag="U4A80_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEN1976510.1"
                     /translation="YAQWIPHPVCRNTIGGAVVVAAALILQTQRYLGLSLPLLQESFN
                     GQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALAPLLHVSVPFLAGLGF
                     IGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGNSGIYSAQQVAVKKGI
                     LFLPIVKRMKEKNKV"
BASE COUNT          137 a           99 c          131 g          179 t
ORIGIN      
        1 tatgcacaat ggattcctca tcctgtttgt cgaaatacga taggtggagc tgtagttgtg
       61 gcagcagcat tgattttgca aacacaacgt tatctcggat tgagtcttcc gttacttcaa
      121 gaatcattta atggacaggc acatgtctat gattttattg ggaagttatt cttcacagtt
      181 ttgtctttag gcgcgggata tcaaggggga gaagtgacac cattgtttga aattggtgca
      241 acgcttggtg cagcactagc gcctttgctc catgtttctg ttccttttct tgcaggattg
      301 ggctttatcg gagtattttc tggtgcgaca aatacaccga ttgcctgctt tgtgatgggg
      361 atcgaattgt tcggttcaca agcggcagta tacttcttta tgatctgctt gatcagcttt
      421 atgtgttcag gcaacagcgg tatttattct gctcagcaag tagcggtcaa aaaagggata
      481 ctctttttgc caatagttaa gagaatgaaa gaaaaaaaca aggtgtgaca tatgttttgt
      541 cacacc
//
LOCUS       DASJRQ010000288          540 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJRQ010000288 DASJRQ010000000
VERSION     DASJRQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.300"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..>540
                     /locus_tag="U4A80_002950"
     CDS             288..>540
                     /locus_tag="U4A80_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1976511.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQ"
BASE COUNT          210 a           79 c           87 g          164 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
//
LOCUS       DASJRQ010000289          538 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJRQ010000289 DASJRQ010000000
VERSION     DASJRQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.301"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>538
                     /locus_tag="U4A80_002951"
     CDS             <1..>538
                     /locus_tag="U4A80_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1976512.1"
                     /translation="YLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYE
                     EGYIIDSITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSL
                     NGNLGNADWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLA
                     NLINQAYQANPEIFGTDE"
BASE COUNT          181 a          108 c          115 g          134 t
ORIGIN      
        1 actacttggc gaagatgaac tacccagcaa atgataattg gggcgttgaa tcggctcctt
       61 tagggacagt agatgtaacg acgcttcaac agacaaatgg attccaggcc ttagcaaatg
      121 gtggagtcta cgaagaaggc tatatcattg attccatcac agataatgca ggtaatgtga
      181 tttataaaca tgaaagtaat tctgtccgta tttattccga agcaacggca tcgatcatga
      241 atgacatgat gcgtagtgtg atcaatgaaa aaatcactac accattcaaa gatgtcatct
      301 caagcttaaa cggaaatcta ggaaatgcag attgggtcgg aaaaactggt tctaccaatg
      361 aataccgaga ttcatggctg atcgtctcaa ctccatctat cactatcagt agttgggcag
      421 gacacgatga caatactgga atggacagcc aagcaagaat acgttcggct aattatcttg
      481 ccaatctaat caatcaagct taccaagcaa accctgagat tttcggcact gatgagaa
//
LOCUS       DASJRQ010000290          536 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJRQ010000290 DASJRQ010000000
VERSION     DASJRQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.302"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..>536
                     /locus_tag="U4A80_002952"
     CDS             96..>536
                     /locus_tag="U4A80_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN1976513.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYSVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH"
BASE COUNT          210 a           90 c          103 g          133 t
ORIGIN      
        1 gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat
       61 ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag
      121 acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta
      181 accgaatggg acgtgctata caaactattt atagtgtagt caataagttc aagcaaggca
      241 agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt gggagaaaag
      301 tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga
      361 cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt
      421 atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa
      481 aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccat
//
LOCUS       DASJRQ010000291          532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJRQ010000291 DASJRQ010000000
VERSION     DASJRQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.303"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>532
                     /locus_tag="U4A80_002953"
     CDS             <1..>532
                     /locus_tag="U4A80_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976514.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPS"
BASE COUNT          198 a           83 c           91 g          160 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc ta
//
LOCUS       DASJRQ010000292          531 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJRQ010000292 DASJRQ010000000
VERSION     DASJRQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.304"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            109..303
                     /locus_tag="U4A80_002954"
     CDS             109..303
                     /locus_tag="U4A80_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976515.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          224 a           68 c           87 g          152 t
ORIGIN      
        1 actttcaaat tgattctttt tatttatcat aaagatatag atacataatc attttcactt
       61 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
      121 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      181 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      241 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      301 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      361 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      421 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      481 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt g
//
LOCUS       DASJRQ010000293          513 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJRQ010000293 DASJRQ010000000
VERSION     DASJRQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.305"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          151 a           81 c           72 g          209 t
ORIGIN      
        1 atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca
       61 cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt
      121 tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt
      181 tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt atattgttac
      241 gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac
      301 tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct
      361 tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt
      421 tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt
      481 attaatttac actcatgttc tttctgtagc tat
//
LOCUS       DASJRQ010000294          505 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJRQ010000294 DASJRQ010000000
VERSION     DASJRQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.306"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>505
                     /locus_tag="U4A80_002955"
     CDS             <1..>505
                     /locus_tag="U4A80_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1976516.1"
                     /translation="KVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPDASG
                     EIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYL
                     VASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKDQEV
                     EAFPMSVC"
BASE COUNT          192 a           52 c          120 g          141 t
ORIGIN      
        1 aaggtaagtg tgatagtacc aatttacaat gttgaaaaat atttaagaaa atgtgtggat
       61 agtttattga gtcagacatt aaaagagata gaaatcgtat tagttgatga tggttcgcca
      121 gatgcttcgg gggaaatagc agatgagtat caaaaaaaat attctaatgt aaaaacaata
      181 catagagaaa atggggggtt aggaccggca agaaatacag gaattgagaa tgcgacagga
      241 gaatatgtgg cttttcttga ttcagatgat tgggtagaaa gtgatatgta tgaaaaatta
      301 tacttagttg cgtccaaaga aaatgctgat atagtagtta gtggacattg tgattttgcg
      361 aatgacaaag ctatggtgaa aaagcaacat cctttgagtg ggaatgtata tgatacgcct
      421 aatagtatta aaaatgtacg aaaaaattta tttggacata gtccaaaaga tcaagaagta
      481 gaggccttcc cgatgtcagt atgta
//
LOCUS       DASJRQ010000295          505 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJRQ010000295 DASJRQ010000000
VERSION     DASJRQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.307"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..171)
                     /locus_tag="U4A80_002956"
     CDS             complement(<1..171)
                     /locus_tag="U4A80_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1976517.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISI"
     gene            complement(190..351)
                     /locus_tag="U4A80_002957"
     CDS             complement(190..351)
                     /locus_tag="U4A80_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1976518.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            421..>505
                     /locus_tag="U4A80_002958"
     CDS             421..>505
                     /locus_tag="U4A80_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1976519.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
BASE COUNT          172 a           97 c           60 g          176 t
ORIGIN      
        1 tattgatatt gaagaattag aactttcata taaagccttt tctatttcat tgcttgattt
       61 cattattgtt aaacaagaat acaagcaaga aagaactata gttaatatga taaaaattat
      121 taaagttcta tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac
      181 tactcctttc tattttctta ctccataact aattgcatcc actggacaag catttttaca
      241 tcgtccacat ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg
      301 gcaaactttt ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac
      361 tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc
      421 atgccaagac gtcaacgaag aacctattca aaagaattca aacaacaaat cgtcgatctc
      481 tatctcgctg gtaagccttg cgcag
//
LOCUS       DASJRQ010000296          493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJRQ010000296 DASJRQ010000000
VERSION     DASJRQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.308"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..>493)
                     /locus_tag="U4A80_002959"
     CDS             complement(54..>493)
                     /locus_tag="U4A80_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1976520.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DASJRQ010000297          489 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJRQ010000297 DASJRQ010000000
VERSION     DASJRQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.309"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..100)
                     /locus_tag="U4A80_002960"
     CDS             complement(<1..100)
                     /locus_tag="U4A80_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1976521.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNISYREVQE"
     gene            200..>489
                     /locus_tag="U4A80_002961"
     CDS             200..>489
                     /locus_tag="U4A80_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1976522.1"
                     /translation="MNNLLEKKQKELIKLANKGQDYEHLADEIDELRDKRQLLLVEDA
                     SLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSG"
BASE COUNT          167 a           97 c           88 g          137 t
ORIGIN      
        1 tttcttgaac ttcacgatag ctaatgttat aacgaagata gtagcccacg gctacaataa
       61 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct tctgctatct
      121 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aaccaacgac
      181 agaataccaa atttcagaga ttaacaactt actggagaaa aagcaaaaag aactcatcaa
      241 gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg agctgagaga
      301 caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa
      361 tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg atgataagct
      421 tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca tatccttcaa
      481 atctggcat
//
LOCUS       DASJRQ010000298          470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJRQ010000298 DASJRQ010000000
VERSION     DASJRQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.310"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>470
                     /locus_tag="U4A80_002962"
     CDS             <1..>470
                     /locus_tag="U4A80_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEN1976523.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DASJRQ010000299          441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJRQ010000299 DASJRQ010000000
VERSION     DASJRQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.311"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..213
                     /gene="ybeY"
                     /locus_tag="U4A80_002963"
     CDS             <1..213
                     /gene="ybeY"
                     /locus_tag="U4A80_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1976524.1"
                     /translation="LPRVLGDIMISTERAAEQAEAYGHSYDRELGFLAVHGFLHINGY
                     DHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            197..>441
                     /locus_tag="U4A80_002964"
     CDS             197..>441
                     /locus_tag="U4A80_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1976525.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENV"
BASE COUNT          127 a           74 c          108 g          132 t
ORIGIN      
        1 ttgcctcgtg tactgggaga catcatgatc tctactgaac gtgctgcaga gcaagcagaa
       61 gcttacggcc attcgtatga tcgggaactt ggctttttag cggttcatgg attcttgcat
      121 atcaacgggt atgaccatat gacaccagag gatgagaaag aaatgtttgg tttacagaaa
      181 gagatcttag atgcctatgg acttaaaaga taaaaacgta gagaaaaaca aacatttcct
      241 gatgtccgtg gaatttgccg tccaaggagt caaaactgtt tttgaggaag aacgcaacat
      301 gaggaaacat gttgcgttcg gaatcctcgc tttgtttatg ggcgtgattt tccaacttga
      361 tcggatagaa tggttatggc tgcttttagc tgtttttctc gtatggatag tagagatcat
      421 caatactgtt tttgaaaatg t
//
LOCUS       DASJRQ010000300          432 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJRQ010000300 DASJRQ010000000
VERSION     DASJRQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.312"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..137
                     /locus_tag="U4A80_002965"
     CDS             <1..137
                     /locus_tag="U4A80_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1976526.1"
                     /translation="EMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK
                     "
     gene            138..>432
                     /locus_tag="U4A80_002966"
     CDS             138..>432
                     /locus_tag="U4A80_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1976527.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQL"
BASE COUNT          164 a           54 c           89 g          125 t
ORIGIN      
        1 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
       61 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
      121 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
      181 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
      241 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
      301 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
      361 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
      421 tcttacaact ag
//
LOCUS       DASJRQ010000301          421 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJRQ010000301 DASJRQ010000000
VERSION     DASJRQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.313"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..416
                     /locus_tag="U4A80_002967"
     CDS             <1..416
                     /locus_tag="U4A80_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1976528.1"
                     /translation="ISLVFQNYNLIDYLSPIENIRLVNKSADESILFELGLDKKQIKR
                     NVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCV
                     IVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          177 a           40 c           76 g          128 t
ORIGIN      
        1 atatatcttt agtatttcaa aactataatt taatagatta tttatcacca attgaaaata
       61 ttagattagt aaataaatca gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa
      121 aacaaataaa aagaaatgtt atgaaattat ctggtggaca gcaacaaaga gtagctattg
      181 ctagagcatt ggtatcagat gctccaataa tactagctga tgagcctact ggtaatctag
      241 acagtgttac tgctggagaa ataattaata tattaaagac attagctaaa gatagaaata
      301 aatgtgtaat agttgtaaca catagtaagg aagtagcaga ttctgcggat atcattttag
      361 aactaagtgg taaaaagttg aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat
      421 a
//
LOCUS       DASJRQ010000302          420 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJRQ010000302 DASJRQ010000000
VERSION     DASJRQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.314"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..391
                     /locus_tag="U4A80_002968"
     CDS             98..391
                     /locus_tag="U4A80_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976529.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          164 a           55 c           82 g          119 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
//
LOCUS       DASJRQ010000303          406 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJRQ010000303 DASJRQ010000000
VERSION     DASJRQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.315"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          145 a           50 c           70 g          141 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaa
//
LOCUS       DASJRQ010000304          400 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJRQ010000304 DASJRQ010000000
VERSION     DASJRQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.316"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U4A80_002969"
     CDS             <1..390
                     /locus_tag="U4A80_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976530.1"
                     /translation="CRQHHGTTLMRYFYVKAKERNPEVTEEQFRYPGPKPQSREAGIV
                     NIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQLDDSGLTLKEIRKVEKSLISG
                     LSSTFHSRIKYPKMKSEVEKIKEEQEE"
BASE COUNT          148 a           73 c           93 g           86 t
ORIGIN      
        1 tgtagacagc atcatggaac cacgctgatg cgttatttct acgttaaggc aaaggaacgc
       61 aacccggaag ttacggaaga gcaattccgt tatcctggac caaaacctca aagccgtgaa
      121 gcaggaatcg tcaatatagc tgacagttgt gaagcggcag tacgggcaat ggaccatcca
      181 acgagcgaaa aaatcgaaag ttttgtccat aatcttattg aagagcgtat ttccgatgga
      241 caattggacg acagtggatt gacgttaaaa gaaatccgta aagtagaaaa atcattgatc
      301 agcggtttaa gcagtacctt ccattctcgg atcaaatatc ctaaaatgaa atcggaagtt
      361 gagaagataa aagaagaaca agaagaatag caagaagaat
//
LOCUS       DASJRQ010000305          393 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJRQ010000305 DASJRQ010000000
VERSION     DASJRQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.317"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..>393
                     /locus_tag="U4A80_002970"
     CDS             200..>393
                     /locus_tag="U4A80_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976531.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEG"
BASE COUNT          153 a           50 c           71 g          119 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg cat
//
LOCUS       DASJRQ010000306          349 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJRQ010000306 DASJRQ010000000
VERSION     DASJRQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.319"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>349
                     /locus_tag="U4A80_002971"
     CDS             <1..>349
                     /locus_tag="U4A80_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1976532.1"
                     /translation="DVISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVID
                     AVLNAEQKDLTKVFAAAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPEL
                     FENDAEKELHKAVE"
BASE COUNT          126 a           65 c           77 g           81 t
ORIGIN      
        1 gatgttatca gtaaagatac ggatcgtttc ggcattggtt tatcagaagg gcaacaacaa
       61 gtgatcgact ttatcaaagg ccgtctgcgc caattattga caacgaagaa tattcgtcat
      121 gatgtcatcg atgctgtttt gaatgcggaa caaaaagatc tgacaaaagt atttgcagca
      181 gcacaattgt tcaaacaaca tcttgcagat gaagacttca aaccttcaat ggaagctttg
      241 acacgtgtcg tgaacttagc gaaaaaagcg cagcttgaaa aacaatcaga agtagaccct
      301 gagctgttcg aaaacgacgc agagaaagaa ttacacaaag cagtagaag
//
LOCUS       DASJRQ010000307          341 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJRQ010000307 DASJRQ010000000
VERSION     DASJRQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.320"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>341
                     /locus_tag="U4A80_002972"
     CDS             <1..>341
                     /locus_tag="U4A80_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976533.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DASJRQ010000308          335 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJRQ010000308 DASJRQ010000000
VERSION     DASJRQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.321"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          125 a           54 c           59 g           97 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaata
//
LOCUS       DASJRQ010000309          322 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJRQ010000309 DASJRQ010000000
VERSION     DASJRQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.322"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..230
                     /locus_tag="U4A80_002973"
     CDS             27..230
                     /locus_tag="U4A80_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEN1976534.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          124 a           37 c           70 g           91 t
ORIGIN      
        1 tatgaaaaag aaagaaaaag aaagtattga aacattgtgt tattctagga atattaggaa
       61 cttgtctagc tggcattggt acaggaatag acgttgacgc agctacttac tatggaaatg
      121 gtctttattg taacaaagaa aaatgttggg taaattgggg acaatcatgg tcagaagggt
      181 taaaacgttg gggagataat ctttttggta gttttagtgg aggacgataa tgacttcaat
      241 aaacattgat cacacaaaaa aagtcataca tgaattatac aatagtttaa tgacacgacc
      301 taatcagacg aaagcattat ta
//
LOCUS       DASJRQ010000310          321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJRQ010000310 DASJRQ010000000
VERSION     DASJRQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.323"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="U4A80_002974"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="U4A80_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DASJRQ010000311          319 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJRQ010000311 DASJRQ010000000
VERSION     DASJRQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.denovo.324"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>319)
                     /locus_tag="U4A80_002975"
     CDS             complement(<1..>319)
                     /locus_tag="U4A80_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1976535.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKEL"
BASE COUNT           90 a           71 c           42 g          116 t
ORIGIN      
        1 ccaattcttt caaaatactg ataaataatt cgtggctttt gcgatcccat tgttttaaga
       61 gacctttctt tgtcttctcc atcgtaatac ctgatccgtc gtatcgtttt aagcgcgcat
      121 ttttgagtac aacattttta atcaaatgtt ccccataaat ctcttgggct tgtttataca
      181 tttcctgatc tactgcgtca ccgctttcca acatgaaagg aataacacca gcaacatata
      241 aatttgtatc aaattctttc acaaaaaagt ccatatactc aagatattgc atcgcatttc
      301 tcatcgattt tacttgggt
//
LOCUS       DASJRQ010000312        13375 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.075, whole genome shotgun sequence.
ACCESSION   DASJRQ010000312 DASJRQ010000000
VERSION     DASJRQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.075"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..577)
                     /locus_tag="U4A80_002976"
     CDS             complement(134..577)
                     /locus_tag="U4A80_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1976536.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U4A80_002977"
     CDS             complement(795..2135)
                     /locus_tag="U4A80_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1976537.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U4A80_002978"
     CDS             2404..3300
                     /locus_tag="U4A80_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1976538.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U4A80_002979"
     CDS             complement(3450..4391)
                     /locus_tag="U4A80_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1976539.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U4A80_002980"
     CDS             4517..5638
                     /locus_tag="U4A80_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1976540.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U4A80_002981"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U4A80_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1976541.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U4A80_002982"
     CDS             7426..7992
                     /locus_tag="U4A80_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1976542.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="U4A80_002983"
     CDS             8203..9264
                     /locus_tag="U4A80_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1976543.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="U4A80_002984"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="U4A80_002984"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1976544.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="U4A80_002985"
     CDS             10177..12159
                     /locus_tag="U4A80_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1976545.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U4A80_002986"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U4A80_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1976546.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..>13375
                     /locus_tag="U4A80_002987"
     CDS             12881..>13375
                     /locus_tag="U4A80_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1976547.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVP"
BASE COUNT         4368 a         2426 c         2799 g         3782 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgcca
//
LOCUS       DASJRQ010000313         7959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.110, whole genome shotgun sequence.
ACCESSION   DASJRQ010000313 DASJRQ010000000
VERSION     DASJRQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.110"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..1564
                     /locus_tag="U4A80_002988"
     CDS             128..1564
                     /locus_tag="U4A80_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1976548.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="U4A80_002989"
     CDS             1753..4200
                     /locus_tag="U4A80_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1976549.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="U4A80_002990"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="U4A80_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1976550.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="U4A80_002991"
     CDS             complement(5690..6793)
                     /locus_tag="U4A80_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1976551.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="U4A80_002992"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="U4A80_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1976552.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DASJRQ010000314         5879 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.125, whole genome shotgun sequence.
ACCESSION   DASJRQ010000314 DASJRQ010000000
VERSION     DASJRQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.125"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            398..862
                     /locus_tag="U4A80_002993"
     CDS             398..862
                     /locus_tag="U4A80_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1976553.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            846..1475
                     /locus_tag="U4A80_002994"
     CDS             846..1475
                     /locus_tag="U4A80_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1976554.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1829..3307
                     /gene="msr(C)"
                     /locus_tag="U4A80_002995"
     CDS             1829..3307
                     /gene="msr(C)"
                     /locus_tag="U4A80_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1976555.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3354..3800
                     /locus_tag="U4A80_002996"
     CDS             3354..3800
                     /locus_tag="U4A80_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1976556.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3999..4514)
                     /locus_tag="U4A80_002997"
     CDS             complement(3999..4514)
                     /locus_tag="U4A80_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1976557.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4631..5833
                     /locus_tag="U4A80_002998"
     CDS             4631..5833
                     /locus_tag="U4A80_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1976558.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1817 a          980 c         1240 g         1842 t
ORIGIN      
        1 ctatttttct tgcacctttt acttgactaa tcgtaggaaa aaggttgtga caacatcttg
       61 tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt
      121 cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct
      181 atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca
      241 ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt
      301 tttttattac acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc
      361 aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca
      421 ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat
      481 cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct
      541 acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa
      601 ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt
      661 gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa
      721 agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac
      781 acttttagat aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa
      841 taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg
      901 tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga
      961 cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg
     1021 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
     1081 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca
     1141 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
     1201 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
     1261 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
     1321 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
     1381 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
     1441 gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag
     1501 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
     1561 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
     1621 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
     1681 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
     1741 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
     1801 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
     1861 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
     1921 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     1981 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     2041 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     2101 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     2161 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     2221 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     2281 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     2341 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     2401 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     2461 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     2521 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
     2581 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     2641 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     2701 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     2761 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     2821 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     2881 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     2941 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     3001 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     3061 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     3121 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     3181 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     3241 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     3301 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     3361 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     3421 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     3481 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     3541 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     3601 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     3661 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     3721 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     3781 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     3841 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     3901 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     3961 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     4021 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     4081 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     4141 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     4201 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     4261 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     4321 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     4381 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     4441 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     4501 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     4561 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     4621 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     4681 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     4741 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     4801 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     4861 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     4921 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     4981 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     5041 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     5101 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     5161 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     5221 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     5281 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     5341 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     5401 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     5461 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     5521 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     5581 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     5641 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     5701 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     5761 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     5821 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DASJRQ010000315         5851 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.126, whole genome shotgun sequence.
ACCESSION   DASJRQ010000315 DASJRQ010000000
VERSION     DASJRQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.126"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            322..1014
                     /locus_tag="U4A80_002999"
     CDS             322..1014
                     /locus_tag="U4A80_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1976559.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="U4A80_003000"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="U4A80_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1976560.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="U4A80_003001"
     CDS             complement(1910..3862)
                     /locus_tag="U4A80_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1976561.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="U4A80_003002"
     CDS             complement(3869..4105)
                     /locus_tag="U4A80_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976562.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="U4A80_003003"
     CDS             4507..4797
                     /locus_tag="U4A80_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1976563.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="U4A80_003004"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="U4A80_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DASJRQ010000316         3815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.161, whole genome shotgun sequence.
ACCESSION   DASJRQ010000316 DASJRQ010000000
VERSION     DASJRQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.161"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            295..1686
                     /gene="amt"
                     /locus_tag="U4A80_003005"
     CDS             295..1686
                     /gene="amt"
                     /locus_tag="U4A80_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1976564.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            2081..2554
                     /gene="dfrF"
                     /locus_tag="U4A80_003006"
     CDS             2081..2554
                     /gene="dfrF"
                     /locus_tag="U4A80_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HEN1976565.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2681..3337
                     /locus_tag="U4A80_003007"
     CDS             2681..3337
                     /locus_tag="U4A80_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEN1976566.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3519..>3815
                     /locus_tag="U4A80_003008"
     CDS             3519..>3815
                     /locus_tag="U4A80_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEN1976567.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLL"
BASE COUNT         1254 a          549 c          802 g         1210 t
ORIGIN      
        1 caattctgat tcatcagtcc cataaattat agagcctttt atatgaagta ttctttattc
       61 cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt tctataaaaa
      121 gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca caacacatca
      181 agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa aacagaagaa
      241 aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat
      301 acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc aatggtttgc
      361 ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa taatatacta
      421 acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg ggtatttggt
      481 ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc ggcagatttc
      541 tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac gattccattt
      601 ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt gatgacagga
      661 gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt ttggaatctg
      721 ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga tcagatggga
      781 tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg ctctttagca
      841 tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca gcatagcagt
      901 cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg ttttaattcc
      961 ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac attcatcgct
     1021 cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa tggctttgat
     1081 tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc ttgtgccggt
     1141 tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt ctgtcatgct
     1201 gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat
     1261 ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa cagccattta
     1321 ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt catcattact
     1381 gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca ctttacaacg
     1441 atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt tggctttttg
     1501 gctatgacca tctcaatggt cgtttcatta tatgaatatc ccacattcgc gacctcaggc
     1561 ttcagcttag tatttttctt gttattaggc ggtttgctat ggtttatccc tgtggcattg
     1621 tgcgcagcag agatggcaac ggtgaaagga tgggaaaaag gtgtttccca aacaaaaaga
     1681 tattaagaaa cagcccatgt ttacctgctt gcaaacaaaa gaatattgct actagactaa
     1741 gagatactac ataagtgtgt tggtatctct tttttgttgc cgtgaaacag cagactattt
     1801 catttctgct tgattacctt aaaacttatt agtctatggt ttgtcaaggg cttgttgcgt
     1861 gagaaatgct gaatatcacc ctttacaaaa cataggctga taagtaactt ttcaaaagca
     1921 gaaaagaaat ggcaatgctt ataacaatat gatataatgc agatgaagac aaaaaaggaa
     1981 tttgtcgagc tggttagttc gagataattt tgagtttgtt aagatgtggt gcacttgatg
     2041 gaatcacagg tacgacatca tattatggag gatgaaatag atgataggtt tgattgttgc
     2101 gaggtcaaag aataatgtta taggcaagaa tggtaatata ccatggaaaa taaagggaga
     2161 acaaaagcaa tttagagagt taacaacggg taatgtggtt attatggggc gaaagtctta
     2221 tgaagaaatc ggtcatccgt tgcctaatag aatgaatatt gttgtttcca ccacaacaga
     2281 gtatcaagga gataatttag tttcagttaa atcattagaa gatgcattat tattggctaa
     2341 aggacgagat gtatacatat ctggtggata tggactattt aaggaagctt tgcaaatagt
     2401 agataaaatg tatatcacag aagtagattt aaatattgaa gatggagata cattctttcc
     2461 agaatttgat atcaatgatt ttgaagtttt gataggggaa acacttggtg aggaagtgaa
     2521 atatacgaga acattttatg taaggaaaaa atgaattgag taaattttgg atttagggtg
     2581 ttttcataaa tatattgctt tttatgcata tataaattgt tgtaagacaa attccagttt
     2641 gtatggggcg ataaaatcag aatttgagag aggattgaat atgaataagg aatggtctga
     2701 acttaataaa acaatgcagg cacaaataaa aaagaaagat acttataaga ggggtattga
     2761 tactttactt actttacgaa gtcagttaat acaaacctta gtatctttca aagaagaatt
     2821 atgcagagaa gattttaact caatcccctt cataaacgct gatggttatc atagtaagac
     2881 gattgcttat tctatttggc atattttccg cattgaagat atagttgtgc atacagtgat
     2941 aaatgaagat gaacaagtat tttttgcagg caattatcaa gagcgtatca attcacctat
     3001 cattacaaca ggaaatgaac tcatgaaaca gcagattgca gacttttcaa aacagcttaa
     3061 tctggaagaa ttatatttat atattttcga agtatgggaa agcactgaga aaatgcttga
     3121 acggttatct tatgatgaat tgaaaagaaa aataccgaaa gaaagaaaag gatacttaga
     3181 atcgttgaat gtagtaaacg acaatgaaaa agcaatttgg ctgattgatt attggtgtaa
     3241 taaagatatt tgtggactaa ttcaaatgcc gttttcaaga cattggatta tgcacacaga
     3301 agcctgtttg cgtataaaga acaagataca ttcataggca aaaatttcag tttgtagaac
     3361 taaggaaatt ggaatttaag gaggaataaa gtatggttag tgattttata agagcagcat
     3421 ttccatggat tttaatgggt ttatttgtag caatcagttg ttcccttatg agtaaaaaag
     3481 agaaataaat tcctgtttgt atttataaaa gagaaaaaat gagaaaagta gttttattta
     3541 ttgccatgag ccttgacgga tatattgcgg acggtaatgg tggagtagct tggctaaatg
     3601 gtcatggaaa tgacaatgaa aacatcgaca cttatacaga atttaccaaa gatatagata
     3661 ctgtcttaat ggggtggaat acttctcatc aggttgttac tgaactttct ccgcaagaat
     3721 gggtatataa taaatttaca acttatgtat taacacataa agagtgcaat tcttcccaac
     3781 aaattcattt tacaagtgaa aatcctgttt tatta
//
LOCUS       DASJRQ010000317         2790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.183, whole genome shotgun sequence.
ACCESSION   DASJRQ010000317 DASJRQ010000000
VERSION     DASJRQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.183"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1311
                     /locus_tag="U4A80_003009"
     CDS             <1..1311
                     /locus_tag="U4A80_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEN1976568.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1441..2613)
                     /locus_tag="U4A80_003010"
     CDS             complement(1441..2613)
                     /locus_tag="U4A80_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HEN1976569.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          979 a          354 c          490 g          967 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaagtcc agactcctgt gtaaaatgct atacaatgtt tttaccattt
     1441 ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat
     1501 aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt
     1561 ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt
     1621 gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc
     1681 gcaagctttt gaatattttg gttgatcgat ataatcatga atcaatcgat ttttagcctc
     1741 acgcgctaag ttaatatctg tgaacttaaa aattccttta acagcttctc tgaaagattt
     1801 tgaatttttt ttaggaatgg tggtaaagat atttcttagg aagtgaactt ggcatctttg
     1861 ccaacttacg ttggtgaagg attttctaat ggcagagact aatcctttgt gcgcatcaga
     1921 aataacgagt tccgtacctt gtaaaccgcg ttcttttagg tattcaaaaa atgttgtcca
     1981 ggtctcttcg ctttcgccac tttgaatcat gaagccgata atttcacggt cgccatcttt
     2041 ggttattcca atcgctatat gacagctttt tgagagtact cgattttctt ctcgtacttt
     2101 tatatagagt acatcggtca ttaagtaagg ataatttttt tctgataata aacgattctg
     2161 ccactcgtta accataggtt ctagctgttc tgttaagcta gaaacgaagg acttagagac
     2221 ggatttacca caaagttctt ccacaatttt tgatacttta cgagttgaaa cgcctgatac
     2281 atacatttcc aacattgaag ccatgagggc tttttcgttt cgttgataac gttcaaacac
     2341 tgtgggtgaa aaatggccat cacgtgttct gggtactttt aattctagcg tgcctacacg
     2401 tgtcgtaaag ctgcgctcat aatagccatt tcgttgactt tgtcggtttt ctgttcgttc
     2461 atattctttt gcttgaatat attctgttcg ttgattttcc attagttgat taaataccgt
     2521 tgttaaaata tttttagaaa cgtcatcctt tacagaatat tcaataatgc tttgaatctc
     2581 ttcgcttttc agtgtaaaat gtacttgggt catgtaaaag tcctcctggg tatgtttttg
     2641 tcgttaaaaa cattgtaccg taaaaggact gttatatggc ctttttactt ttacacaatt
     2701 atacggactt tatctatata aaggcgctgt cagagttttt tgacttagaa catacattca
     2761 ttcaagagac tttaaacaag ctagcagaac
//
LOCUS       DASJRQ010000318         2533 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.193, whole genome shotgun sequence.
ACCESSION   DASJRQ010000318 DASJRQ010000000
VERSION     DASJRQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.193"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..304
                     /locus_tag="U4A80_003011"
     CDS             <1..304
                     /locus_tag="U4A80_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEN1976570.1"
                     /translation="EDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEY
                     KPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI"
     gene            285..1019
                     /locus_tag="U4A80_003012"
     CDS             285..1019
                     /locus_tag="U4A80_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1976571.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1052..1960
                     /locus_tag="U4A80_003013"
     CDS             1052..1960
                     /locus_tag="U4A80_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEN1976572.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1957..2499
                     /gene="sat4"
                     /locus_tag="U4A80_003014"
     CDS             1957..2499
                     /gene="sat4"
                     /locus_tag="U4A80_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEN1976573.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          889 a          361 c          558 g          725 t
ORIGIN      
        1 agaggataaa tattatattg caggccatgt ttttcgtatt atttcatgct taaatcaagt
       61 actatttgca tgtaataatg cttattgcat taacgaaaag aaagctataa aactgcttga
      121 aacttttgaa tataaacctg aaaaatatgc cgaaagagtt aatcatattt ttgaagtact
      181 cggtctttct ctttttgaat gctacgacat gaccgagaaa ctttataaag aagtgaatga
      241 aattgtatcg gagataaata actttttaaa cgaggagagt tcagatgaaa gaaaacaaat
      301 atgatgataa tatatttttt caaaaataca gtcaaatgag tcgctcgcag aaaggactgg
      361 ctggtgcggg agaatgggag actttgaaaa agatgctacc tgattttaag ggtaagcgtg
      421 tgcttgattt aggatgcggc tatggatggc actgtatata tgcgatggaa aacggtgctt
      481 cctctgtagt aggtgttgat atttctcata aaatgctcga agtagcaaaa ggaaaaaccc
      541 attttccaca gattgaatat gaatgctgtg ccatagaaga tgtggatttc ccagaggaga
      601 gctttgatgt aatactaagt tcgcttgcgt ttcattatgt agcagactat gagaatttaa
      661 taaaaaagat atataggatg ctgaaggctg gtggcaattt agtttttaca gttgaacatc
      721 ctgtttttac tgctcatgga acacaagact ggtattataa cgaaaaagga gaaatactgc
      781 atttcccggt ggacaattat tattatgagg gcaaacggac agctatgttt ttggaagaaa
      841 aggttacaaa atatcataga acactgacca catatctaaa tacactgctt tcaaatagtt
      901 ttataataaa tcagattgtg gagccacagc cgccagagaa catgatggat attccgggga
      961 tggcggatga aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat
     1021 aatatagaaa aaataaacga ggagtatgta aatgagatca gaaaaagaaa tgatggattt
     1081 agtactttct ttagcagaac aggatgaacg tattcgaatt gtgacccttg aggggtcacg
     1141 cgcaaatatt aatataccta aagatgaatt tcaggattat gatattacat attttgtaag
     1201 tgatatagaa ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat
     1261 gatgcaaaag ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct
     1321 tatgctattt gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga
     1381 taattaccta aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa
     1441 aagggacata gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta
     1501 tgatgattgc tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg
     1561 taaggaaatt ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag
     1621 aatgatatca tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta
     1681 taagtttatt gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg
     1741 gatggattcc tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc
     1801 ggtatccggt gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat
     1861 aacaaaatat accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag
     1921 cacatatgcc gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg
     1981 cacctgaaag atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg
     2041 aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa
     2101 agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac
     2161 aatactgata agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa
     2221 ctgcgaaaaa attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc
     2281 agggggcaag gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa
     2341 aacttgcatg gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat
     2401 cataattgtg gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac
     2461 aactttgaaa aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg
     2521 gagttcgtct tgt
//
LOCUS       DASJRQ010000319         2312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.199, whole genome shotgun sequence.
ACCESSION   DASJRQ010000319 DASJRQ010000000
VERSION     DASJRQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.199"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            70..807
                     /gene="erm(B)"
                     /locus_tag="U4A80_003015"
     CDS             70..807
                     /gene="erm(B)"
                     /locus_tag="U4A80_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1976574.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1070..1933)
                     /locus_tag="U4A80_003016"
     CDS             complement(1070..1933)
                     /locus_tag="U4A80_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1976575.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1935..2207)
                     /locus_tag="U4A80_003017"
     CDS             complement(1935..2207)
                     /locus_tag="U4A80_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1976576.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2224..>2312)
                     /locus_tag="U4A80_003018"
     CDS             complement(2224..>2312)
                     /locus_tag="U4A80_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1976577.1"
                     /translation="TAKNGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          705 a          410 c          376 g          821 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct atagatgtaa tcacttcagg
       61 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      121 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      181 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      241 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa cactcgtgtc
      301 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      361 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      421 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
      481 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
      541 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
      601 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
      661 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
      721 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
      781 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
      841 acacgttact aaagggaatg tagataaatt attaggtata gggcacctct aataactacc
      901 aattaattca cctcaaaatg aaattagaaa aaaacagcga aatttttaaa tctatttctt
      961 atcgatacaa attcctcgta ggcgctcggg accccaacat gaggaaatgt ttttataaaa
     1021 aaagagtagt taccgttttt tggtaactac tcttttcctt ttttaaggtt taaatacctg
     1081 gaagtttagg tgttttgggt attggtggtg taggtagctg caagctttcc attttttgtt
     1141 gaattgcttt aaattcaggg gtttcttggt gttgattttg aaccattttt tgctctattc
     1201 tctccaaagt cggttgaatt tctttccctg atactttacg atttaattct ctttctaagg
     1261 tctctttcgg actaatggaa ggcgtttcta agcttgaata gagtttcact ccttctctat
     1321 tgtaaagcct tatatcgcta aataagcccg ttttatgaag ggtttctaaa ttggtcggta
     1381 agtttttgac aacaatatca tgcgcttgtt ttggtgttgc cctagctgtc attggatcat
     1441 ctgcatacat ggtttcatat cgttcaatcg ttcctaagta tgattcaatt ttcggtactg
     1501 ccatgacgta tatttttgtt tcataacctt tagattgaag cattgtggct gtttgaatgg
     1561 gaacgtctgt tgttcgtcct gttccttcaa tcactaaatt atagccttga tcgcttaaac
     1621 ggctaataag tgcttctgtc atgcgattcg aataaggggt aacgtgtttt actacgtctt
     1681 tttcatagag tttcactaat tcgtcaaaat ttgggtgctg ttgtttaaag gtatcattat
     1741 caatgacaat aacattccct tgtgtttctt caaaaattgc tgatcgcaaa ctggttttcc
     1801 ctgaccctgg ttgcccacca agtaaaaaag cggttggcga ttcaaccgct agtctatttt
     1861 tagttagtcg ttcaacattc ttttctaaac gatcttcaaa ttgcttttcg gtgaagtcag
     1921 taatattcgc catgttaagc cactttctct ttattcaaaa tttgttttga atattgattc
     1981 attgaccgcc aatactcatg aatagcttgt aattcttcaa atggttgttg aaataaatca
     2041 acttcttgtg ccacttctaa ttgatttaaa agattcactt ctagtaaacg agtattttta
     2101 gtatctagtt catgattcaa aacataattt aatacccgat tataaacact tgccgataac
     2161 tcattaatga tttcaatttc aaatgttttt tcatacgtaa ctgccatttc attttcactc
     2221 cttttaaagt ttgtcaggta aatatttccg tatatattct tctaaggctt gatccataac
     2281 ttcttttacg tttccgccat tctttgctgt tt
//
LOCUS       DASJRQ010000320         1909 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.213, whole genome shotgun sequence.
ACCESSION   DASJRQ010000320 DASJRQ010000000
VERSION     DASJRQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.213"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..893
                     /locus_tag="U4A80_003019"
                     /note="aph(3')-IIIa"
     CDS             99..893
                     /locus_tag="U4A80_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1976578.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1169..1741
                     /locus_tag="U4A80_003020"
     CDS             1169..1741
                     /locus_tag="U4A80_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1976579.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT          634 a          306 c          455 g          514 t
ORIGIN      
        1 ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt
       61 atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac
      121 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
      181 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
      241 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
      301 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
      361 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
      421 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
      481 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
      541 ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc
      601 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
      661 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
      721 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
      781 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
      841 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag
      901 atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt
      961 acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagataga
     1021 gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa
     1081 ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt
     1141 tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct
     1201 tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat
     1261 aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa taccaattgg
     1321 atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc cggtaatgtt
     1381 tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt gccatggaga
     1441 tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta ttttgaagca
     1501 tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca gcacatctgg
     1561 acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa tctgcaataa
     1621 aagggtaata tcaattcaat atcctgcatc aggagggcaa gaaccggaaa gcagaatgat
     1681 agagcctata tcactggggt tttatgaaca gaactggtac ttaatcggtt ttgcaggcta
     1741 agaaatgaat ataggaattt tcgtgtggat cggattaaaa gcatttgcat agaagaagag
     1801 ctctgtcaaa gccatgatgg ctctttggaa caaatattaa aacaaatgct ttcatataaa
     1861 aaactatatg ccctacaatg ccgttgtaaa aattggggaa aagggatat
//
LOCUS       DASJRQ010000321         1121 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.248, whole genome shotgun sequence.
ACCESSION   DASJRQ010000321 DASJRQ010000000
VERSION     DASJRQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.248"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..939
                     /locus_tag="U4A80_003021"
     CDS             28..939
                     /locus_tag="U4A80_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEN1976580.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          413 a          151 c          227 g          330 t
ORIGIN      
        1 actatatagt taaggaggag actgaaaatg aagaagttgt tttttttatt gttattgtta
       61 ttcttaatat acttaggtta tgactacgtt aatgaagcac tgttttctca ggaaaaagtc
      121 gaatttcaaa attatgatca aaatcccaaa gaacatttag aaaatagtgg gacttctgaa
      181 aatacccaag agaaaacaat tacagaagaa caggtttatc aaggaaatct gctattaatc
      241 aatagtaaat atcctgttcg ccaagaaagt gtgaagtcag atatcgtgaa tttatctaaa
      301 catgacgaat taataaatgg atacgggttg cttgatagta atatttatat gtcaaaagaa
      361 atagcacaaa aattttcaga gatggtcaat gatgctgtaa agggtggcgt tagtcatttt
      421 attattaata gtggctatcg agactttgat gagcaaagtg tgctttacca agaaatgggg
      481 gctgagtatg ccttaccagc aggttatagt gagcataatt caggtttatc actagatgta
      541 ggatcaagct tgacgaaaat ggaacgagcc cctgaaggaa agtggataga agaaaatgct
      601 tggaaatacg ggttcatttt acgttatcca gaggacaaaa cagagttaac aggaattcaa
      661 tatgaaccat ggcatattcg ctatgttggt ttaccacata gtgcgattat gaaagaaaag
      721 aatttcgttc tcgaggaata tatggattac ctaaaagaag aaaaaaccat ttctgttagt
      781 gtaaatgggg aaaaatatga gatcttttat tatcctgtta ctaaaaatac caccattcat
      841 gtgccgacta atcttcgtta tgagatatca ggaaacaata tagacggtgt aattgtgaca
      901 gtgtttcccg gatcaacaca tactaattca aggaggtaag gatggcggaa tgaaaccaac
      961 gaaattaatg aacagcatta ttgtactagc acttttgggg taacgttagc tttttaattt
     1021 aaaacccacg ttaactagga cattgctata ctaatgatac aacttaaaca aaagaattag
     1081 aggaaattat attgggaaaa atattatcta gaggattgct a
//
LOCUS       DASJRQ010000322          875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.268, whole genome shotgun sequence.
ACCESSION   DASJRQ010000322 DASJRQ010000000
VERSION     DASJRQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.268"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..519
                     /gene="aac(6')-I"
                     /locus_tag="U4A80_003022"
                     /pseudo
     CDS             7..519
                     /gene="aac(6')-I"
                     /locus_tag="U4A80_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
     gene            550..>875
                     /locus_tag="U4A80_003023"
     CDS             550..>875
                     /locus_tag="U4A80_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HEN1976581.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQN"
BASE COUNT          371 a           72 c          144 g          288 t
ORIGIN      
        1 aatgaaatat gtataagaac tttaatagat gatgattttc ctttgatgtt aaaatggtta
       61 actgatgaaa gagtattaga attttatgat ggtagagata aaaaatatac attagaatca
      121 ttaaaaaaac attatacaga gccttgggaa gatgaagttt ttagagtaat tattgaatat
      181 aacaatgttc ctattggata tggacaaata tataaaatgt atgatgagtt atatactgat
      241 tatcattatc caaaaactga tgagatagtc tatggtatgg atcaatttat aggagagcca
      301 aattattgga gtaaaggaat tggtacaaga tatattaaat tgatttttga atttttgaaa
      361 aaagaaagaa atgctaatgc agttatttta gaccctcata aaaataatcc aagagcaata
      421 agggcatacc aaaaatctgg ttttagaatt attgaagatt tgccagaaca tgaattacac
      481 gagggcaaaa aagaagattg ttatttaatg gaatatagat atgatgataa tgccacaaat
      541 gttaaggcaa tgaaatattt aattgagcat tactttgata atttcaaagt agatagtatt
      601 gaaataatcg gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt
      661 aaaacaaaat ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat
      721 tttttaaata caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt
      781 agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa
      841 atttattcta ctatgtcaga agaagaacaa aattt
//
LOCUS       DASJRQ010000323          861 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3894
            SAMN38756289-rid20573523.guided.270, whole genome shotgun sequence.
ACCESSION   DASJRQ010000323 DASJRQ010000000
VERSION     DASJRQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756289
            Sequence Read Archive: SRR27168019
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028181.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028181.1
            Genome Coverage       :: 138x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:57:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,024
            CDSs (total)                      :: 2,967
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 26 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 16 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756289-rid20573523.guided.270"
                     /isolate="VRE3894"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2018-08-13"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..406
                     /locus_tag="U4A80_003024"
     CDS             <1..406
                     /locus_tag="U4A80_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1976582.1"
                     /translation="NILSVFNYHQRSLNLTPFTATGNFREMIDNVIIFIPFGLLLNVN
                     FKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATDITDVITNTVGGFLGLKLYGLSNKHM
                     NQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
BASE COUNT          287 a          131 c          149 g          294 t
ORIGIN      
        1 caatatttta tcagtattta attatcatca aagaagtctt aacttgactc catttactgc
       61 tactgggaat ttcagagaga tgatagataa tgttataatc tttattccat ttggcttgct
      121 tttgaatgtc aattttaaag aaatcggatt tttacctaag tttgcttttg tactggtttt
      181 aagtcttact tttgaaataa ttcaatttat cttcgctatt ggagcgacag acataacaga
      241 tgtaattaca aatactgttg gaggctttct tggactgaaa ttatatggtt taagcaataa
      301 gcatatgaat caaaaaaaat tagacagagt tattattttt gtaggtatac ttttgctcgt
      361 attattgctc gtttaccgta cccatttaag aataaattac gtgtaagatg tctaaatcaa
      421 gcaatctgat ctttcataca cataaagata ttgaatgaat tggattagat ggaaaacggg
      481 atgtggggaa actcgcccgt aggtgtgaag tgaggggaaa accggtgata aagtaaaaag
      541 cttacctaac actatagtaa caaagaaagc ccaattatca attttagtgc tgaggaattg
      601 gtctctttaa taaatttcct taacgttgta aatccgcatt ttcctgacgg tacccccact
      661 agcagccatg ctttttttga atctttgtag gacgttttaa agagaattac ccactttaaa
      721 attaaacatg cgcttagaat cgctttagga aacacgatcc agtccaataa tcgtcgataa
      781 aaacttttga aaaaggttgg tgaaattacc tacttttgga ataatcacaa atcacaagtg
      841 attaatcact tgtttattaa g
//
